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POU3F4 cDNA ORF clone, Homo sapiens (human)

Gene Symbol POU3F4
Entrez Gene ID 5456
Full Name POU class 3 homeobox 4
Synonyms BRAIN-4, BRN-4, BRN4, DFN3, DFNX2, OCT-9, OTF-9, OTF9
General protein information
Preferred Names
POU domain, class 3, transcription factor 4
Names
POU domain, class 3, transcription factor 4
octamer-binding transcription factor 9
brain-specific homeobox/POU domain protein 4
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xq21.1

Summary This gene encodes a member of the POU-III class of neural transcription factors. This family member plays a role in inner ear development. The protein is thought to be involved in the mediation of epigenetic signals which induce striatal neuron-precursor differentiation. Mutations in this gene are associated with X chromosome-linked nonsyndromic mixed deafness. [provided by RefSeq, Dec 2012]. lac of sum
Disorder MIM:

300039

Disorder Html: Deafness, X-linked 2, 304400 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000307 NP_000298 POU domain, class 3, transcription factor 4


Homo sapiens (human) POU3F4 NP_000298.3
Pan troglodytes (chimpanzee) POU3F4 XP_003317585.1
Macaca mulatta (Rhesus monkey) POU3F4 XP_001104059.1
Canis lupus familiaris (dog) POU3F4 XP_549108.1
Bos taurus (cattle) POU3F4 XP_002699986.1
Mus musculus (house mouse) Pou3f4 NP_032927.1
Rattus norvegicus (Norway rat) Pou3f4 NP_058948.1
Xenopus (Silurana) tropicalis (western clawed frog) pou3f4 NP_001090728.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POU3F4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POU3F4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu20520
NM_000307 Homo sapiens POU class 3 homeobox 4 (POU3F4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$149.50-$209.30
$299.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20520
Clone ID Related Accession (Same CDS sequence) NM_000307
Accession Version NM_000307.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1086bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 04-MAY-2014
Organism Homo sapiens (human)
Product POU domain, class 3, transcription factor 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK314967.1, Z82170.3 and X82324.1. This sequence is a reference standard in the RefSeqGene project. On Dec 26, 2012 this sequence version replaced gi:194440735. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCCACAG CTGCCTCGAA TCCCTACAGC ATTCTCAGTT CCACCTCCCT AGTCCATGCG 
GACTCTGCGG GCATGCAGCA GGGGAGTCCT TTCCGCAACC CTCAGAAACT TCTCCAAAGT
GATTACTTGC AGGGAGTTCC CAGCAATGGG CATCCCCTCG GGCATCACTG GGTGACCAGT
CTGAGCGACG GGGGCCCATG GTCCTCCACA CTGGCCACCA GCCCCCTGGA CCAGCAGGAC
GTGAAGCCCG GGCGCGAAGA CCTGCAACTG GGTGCGATCA TCCATCACCG CTCGCCACAC
GTAGCCCACC ACTCACCGCA CACTAACCAC CCCAACGCCT GGGGGGCCAG CCCGGCACCG
AACCCGTCTA TCACGTCAAG CGGCCAACCC CTCAACGTGT ACTCGCAGCC TGGCTTCACC
GTGAGCGGCA TGCTGGAACA CGGGGGACTC ACCCCACCTC CAGCTGCCGC CTCTGCACAG
AGCCTGCACC CGGTGCTCCG AGAGCCCCCG GATCACGGCG AACTGGGCTC GCACCATTGC
CAGGATCACT CCGACGAGGA GACGCCAACC TCTGATGAGT TGGAACAGTT CGCCAAACAA
TTCAAACAAA GAAGAATCAA GTTGGGCTTC ACGCAGGCCG ACGTGGGGTT GGCGCTGGGC
ACACTGTATG GTAACGTGTT CTCGCAGACC ACCATCTGCA GGTTCGAGGC CTTGCAGCTG
AGCTTCAAAA ATATGTGCAA GCTGAAGCCC CTGCTGAACA AGTGGCTGGA GGAGGCGGAT
TCGTCCACAG GGAGCCCGAC CAGCATTGAC AAGATCGCTG CACAGGGCCG CAAGCGCAAG
AAGCGGACCT CCATCGAGGT GAGTGTCAAG GGCGTACTGG AGACGCATTT CCTCAAGTGT
CCCAAGCCTG CCGCGCAGGA GATCTCCTCG CTGGCAGACA GCCTCCAGTT GGAGAAGGAA
GTGGTGCGTG TCTGGTTCTG TAATCGAAGA CAAAAAGAGA AAAGAATGAC TCCGCCAGGG
GATCAGCAGC CGCATGAGGT TTATTCGCAC ACCGTGAAAA CAGACACATC TTGCCATGAT
CTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000298.3
CDS65..1150
Misc Feature(1)14..16(+)
Misc Feature(2)620..844(+)
Misc Feature(3)899..1072(+)
Misc Feature(4)899..1069(+)
Misc Feature(5)905..1057(+)
Exon (1)1..1507
Gene:POU3F4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000307
Organism Homo sapiens (human)
Definition Homo sapiens POU class 3 homeobox 4 (POU3F4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000307

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCCACAG CTGCCTCGAA TCCCTACAGC ATTCTCAGTT CCACCTCCCT AGTCCATGCG 
GACTCTGCGG GCATGCAGCA GGGGAGTCCT TTCCGCAACC CTCAGAAACT TCTCCAAAGT
GATTACTTGC AGGGAGTTCC CAGCAATGGG CATCCCCTCG GGCATCACTG GGTGACCAGT
CTGAGCGACG GGGGCCCATG GTCCTCCACA CTGGCCACCA GCCCCCTGGA CCAGCAGGAC
GTGAAGCCCG GGCGCGAAGA CCTGCAACTG GGTGCGATCA TCCATCACCG CTCGCCACAC
GTAGCCCACC ACTCACCGCA CACTAACCAC CCCAACGCCT GGGGGGCCAG CCCGGCACCG
AACCCGTCTA TCACGTCAAG CGGCCAACCC CTCAACGTGT ACTCGCAGCC TGGCTTCACC
GTGAGCGGCA TGCTGGAACA CGGGGGACTC ACCCCACCTC CAGCTGCCGC CTCTGCACAG
AGCCTGCACC CGGTGCTCCG AGAGCCCCCG GATCACGGCG AACTGGGCTC GCACCATTGC
CAGGATCACT CCGACGAGGA GACGCCAACC TCTGATGAGT TGGAACAGTT CGCCAAACAA
TTCAAACAAA GAAGAATCAA GTTGGGCTTC ACGCAGGCCG ACGTGGGGTT GGCGCTGGGC
ACACTGTATG GTAACGTGTT CTCGCAGACC ACCATCTGCA GGTTCGAGGC CTTGCAGCTG
AGCTTCAAAA ATATGTGCAA GCTGAAGCCC CTGCTGAACA AGTGGCTGGA GGAGGCGGAT
TCGTCCACAG GGAGCCCGAC CAGCATTGAC AAGATCGCTG CACAGGGCCG CAAGCGCAAG
AAGCGGACCT CCATCGAGGT GAGTGTCAAG GGCGTACTGG AGACGCATTT CCTCAAGTGT
CCCAAGCCTG CCGCGCAGGA GATCTCCTCG CTGGCAGACA GCCTCCAGTT GGAGAAGGAA
GTGGTGCGTG TCTGGTTCTG TAATCGAAGA CAAAAAGAGA AAAGAATGAC TCCGCCAGGG
GATCAGCAGC CGCATGAGGT TTATTCGCAC ACCGTGAAAA CAGACACATC TTGCCATGAT
CTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Cytoplasmic mislocalization of POU3F4 due to novel mutations leads to deafness in humans and mice
Hum. Mutat. 34 (8), 1102-1110 (2013)
Parzefall T, Shivatzki S, Lenz DR, Rathkolb B, Ushakov K, Karfunkel D, Shapira Y, Wolf M, Mohr M, Wolf E, Sabrautzki S, de Angelis MH, Frydman M, Brownstein Z and Avraham KB.


book

Destabilization and mislocalization of POU3F4 by C-terminal frameshift truncation and extension mutation
Hum. Mutat. 34 (2), 309-316 (2013)
Choi BY, Kim DH, Chung T, Chang M, Kim EH, Kim AR, Seok J, Chang SO, Bok J, Kim D, Oh SH and Park WY.


book

Common molecular etiologies are rare in nonsyndromic Tibetan Chinese patients with hearing impairment
PLoS ONE 7 (2), E30720 (2012)
Yuan Y, Zhang X, Huang S, Zuo L, Zhang G, Song Y, Wang G, Wang H, Huang D, Han D and Dai P.


book

Nonsyndromic X-linked hearing loss
Front Biosci (Elite Ed) 4, 924-933 (2012)
Song MH, Lee KY, Choi JY, Bok J and Kim UK.


book

The class III POU factor Brn-4 interacts with other class III POU factors and the heterogeneous nuclear ribonucleoprotein U
Brain Res. Mol. Brain Res. 45 (1), 99-107 (1997)
Malik KF, Jaffe H, Brady J and Young WS 3rd.


book

Transcribed sequences encoded in the region involved in contiguous deletion syndrome that comprises X-linked stapes fixation and deafness
Somat. Cell Mol. Genet. 22 (6), 511-517 (1996)
Kandpal G, Jacob AN and Kandpal RP.


book

Transcription factors in disease
Curr. Opin. Genet. Dev. 6 (3), 334-342 (1996)
Engelkamp D and van Heyningen V.


book

Further mutations in Brain 4 (POU3F4) clarify the phenotype in the X-linked deafness, DFN3
Hum. Mol. Genet. 4 (8), 1467-1469 (1995)
Bitner-Glindzicz M, Turnpenny P, Hoglund P, Kaariainen H, Sankila EM, van der Maarel SM, de Kok YJ, Ropers HH, Cremers FP and Pembrey M.


book

Association between X-linked mixed deafness and mutations in the POU domain gene POU3F4
Science 267 (5198), 685-688 (1995)
de Kok YJ, van der Maarel SM, Bitner-Glindzicz M, Huber I, Monaco AP, Malcolm S, Pembrey ME, Ropers HH and Cremers FP.


book

The brain-specific POU-box gene Brn4 is a sex-linked transcription factor located on the human and mouse X chromosomes
Mamm. Genome 5 (3), 180-182 (1994)
Douville PJ, Atanasoski S, Tobler A, Fontana A and Schwab ME.


 
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