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EGLN1 egl-9 family hypoxia-inducible factor 1 [Homo sapiens (human)]

Gene Symbol EGLN1
Entrez Gene ID 54583
Full Name egl-9 family hypoxia-inducible factor 1
Synonyms C1orf12, ECYT3, HALAH, HIF-PH2, HIFPH2, HPH-2, HPH2, PHD2, SM20, ZMYND6
General protein information
Preferred Names
egl nine homolog 1
Names
egl nine homolog 1
egl nine-like protein 1
HIF prolyl hydroxylase 2
HIF-prolyl hydroxylase 2
zinc finger MYND domain-containing protein 6
hypoxia-inducible factor prolyl hydroxylase 2
prolyl hydroxylase domain-containing protein 2
NP_071334.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q42.1

Summary The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). [provided by RefSeq, Nov 2009]. lac of sum
Disorder MIM:

606425

Disorder Html: Erythrocytosis, familial, 3, 609820 (3)





The following EGLN1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EGLN1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu35042 XM_005273166 PREDICTED: Homo sapiens egl-9 family hypoxia-inducible factor 1 (EGLN1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $319
OHu35043 XM_005273167 PREDICTED: Homo sapiens egl-9 family hypoxia-inducible factor 1 (EGLN1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $319
OHu22997 NM_022051 Homo sapiens egl-9 family hypoxia-inducible factor 1 (EGLN1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $359

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu35042
Accession Version XM_005273166.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1152bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product egl nine homolog 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167186.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578802250. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGCCAATG ACAGCGGCGG GCCCGGCGGG CCGAGCCCGA GCGAGCGAGA CCGGCAGTAC 
TGCGAGCTGT GCGGGAAGAT GGAGAACCTG CTGCGCTGCA GCCGCTGCCG CAGCTCCTTC
TACTGCTGCA AGGAGCACCA GCGTCAGGAC TGGAAGAAGC ACAAGCTCGT GTGCCAGGGC
AGCGAGGGCG CCCTCGGCCA CGGAGTGGGC CCACACCAGC ATTCCGGCCC CGCGCCGCCG
GCTGCAGTGC CGCCGCCCAG GGCCGGGGCC CGGGAGCCCA GGAAGGCAGC GGCGCGCCGG
GACAACGCCT CCGGGGACGC GGCCAAGGGA AAAGTAAAGG CCAAGCCCCC GGCCGACCCA
GCGGCGGCCG CGTCGCCGTG TCGTGCGGCC GCCGGCGGCC AGGGCTCGGC GGTGGCTGCC
GAAGCCGAGC CCGGCAAGGA GGAGCCGCCG GCCCGCTCAT CGCTGTTCCA GGAGAAGGCG
AACCTGTACC CCCCAAGCAA CACGCCCGGG GATGCGCTGA GCCCCGGCGG CGGCCTGCGG
CCCAACGGGC AGACGAAGCC CCTGCCGGCG CTGAAGCTGG CGCTCGAGTA CATCGTGCCG
TGCATGAACA AGCACGGCAT CTGTGTGGTG GACGACTTCC TCGGCAAGGA GACCGGACAG
CAGATCGGCG ACGAGGTGCG CGCCCTGCAC GACACCGGGA AGTTCACGGA CGGGCAGCTG
GTCAGCCAGA AGAGTGACTC GTCCAAGGAC ATCCGAGGCG ATAAGATCAC CTGGATCGAG
GGCAAGGAGC CCGGCTGCGA AACCATTGGG CTGCTCATGA GCAGCATGGA CGACCTGATA
CGCCACTGTA ACGGGAAGCT GGGCAGCTAC AAAATCAATG GCCGGACGAA AGCCATGGTT
GCTTGTTATC CGGGCAATGG AACGGGTTAT GTACGTCATG TTGATAATCC AAATGGAGAT
GGAAGATGTG TGACATGTAT ATATTATCTT AATAAAGACT GGGATGCCAA GGTAAGTGGA
GGTATACTTC GAATTTTTCC AGAAGGCAAA GCCCAGTTTG CTGACATTGA ACCCAAATTT
GATAGACTGC TGTTTTTCTG GTCTGACCGT CGCAACCCTC ATGAAGTACA ACCAGCATAT
GCTACAAGGT GA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005273223.1
CDS429..1580
Misc Feature(1)489..602(+)
Misc Feature(2)1086..1568(+)
Translation
Position Chain Variation Link
9 9 c, g dbSNP:533281866
50 50 a, c, g, t dbSNP:533584823
52 52 a, g dbSNP:564650969
55 55 c, t dbSNP:551144196
95 95 c, t dbSNP:531019844
96 96 a, g dbSNP:562125893
121 121 c, t dbSNP:542211420
183 183 c, t dbSNP:573128718
274 274 g, t dbSNP:12049545
368 368 a, g dbSNP:540141256
388 388 a, g dbSNP:772541408
389 389 c, t dbSNP:746436779
392 392 c, g dbSNP:779132675
403 403 c, t dbSNP:757708476
405 405 c, g dbSNP:577443284
406 406 c, t dbSNP:557526823
409 409 c, t dbSNP:756211725
414 414 -, tcgccg dbSNP:759405757
416 416 a, c, t dbSNP:767744500
418 418 c, t dbSNP:754940670
423 423 -, gccgcc dbSNP:776826207
428 428 -, gcc, gccgcc, gccgccgcc dbSNP:574288484
440 440 c, g dbSNP:186996510
452 452 c, t dbSNP:766080154
453 453 c, g dbSNP:762808532
455 455 c, t dbSNP:575002938
464 464 c, g dbSNP:764903617
469 469 a, g dbSNP:761497751
470 470 c, g dbSNP:776101026
495 495 c, t dbSNP:768214321
505 505 a, g dbSNP:746356152
507 507 a, t dbSNP:774928155
515 515 c, t dbSNP:771144835
524 524 c, t dbSNP:749684908
531 531 c, g dbSNP:778100040
548 548 c, t dbSNP:555121182
555 555 a, t dbSNP:748429073
559 559 a, g dbSNP:781405202
568 568 a, g dbSNP:73121547
574 574 a, g dbSNP:755147883
576 576 a, g dbSNP:751382793
583 583 a, g dbSNP:566308354
584 584 a, g dbSNP:766365190
594 594 c, t dbSNP:758090254
596 596 c, t dbSNP:750205790
597 597 a, g dbSNP:765070023
611 611 c, t dbSNP:761442296
618 618 a, g dbSNP:776333127
629 629 c, t dbSNP:377685277
630 630 c, g dbSNP:763530007
632 632 a, g dbSNP:760247413
637 637 a, g dbSNP:774670831
655 655 g, t dbSNP:367712964
673 673 c, t dbSNP:771498926
703 703 a, c dbSNP:553659934
708 708 a, g dbSNP:539743848
715 715 c, t dbSNP:113401862
739 739 c, t dbSNP:551207815
747 747 g, t dbSNP:531081279
808 808 c, g dbSNP:12097901
810 810 c, g dbSNP:756094298
824 824 c, t dbSNP:748371954
833 833 c, t dbSNP:781566823
847 847 c, t dbSNP:768557500
852 852 a, g dbSNP:747146652
859 859 c, t dbSNP:780040550
873 873 a, c dbSNP:374893731
877 877 c, t dbSNP:758337994
879 879 a, g dbSNP:750421640
881 881 c, g dbSNP:778676941
883 883 a, g dbSNP:757050785
891 891 c, t dbSNP:753433323
898 898 a, g dbSNP:748272071
899 899 a, c, g dbSNP:61750991
900 900 -, gag dbSNP:747045922
905 905 a, g dbSNP:766985056
911 911 c, t dbSNP:763502578
917 917 c, t dbSNP:773919305
919 919 c, t dbSNP:770213782
920 920 a, c, g dbSNP:776956500
921 921 a, c, g dbSNP:200502960
922 922 c, g dbSNP:780171999
931 931 c, t dbSNP:772111647
933 933 c, t dbSNP:745738740
935 935 c, g, t dbSNP:756999294
938 938 a, g dbSNP:753663555
943 943 c, t dbSNP:777466821
944 944 a, g dbSNP:755769412
950 950 a, c, t dbSNP:767045832
952 952 c, g dbSNP:759117988
953 953 c, t dbSNP:750900131
961 961 g, t dbSNP:765865681
966 966 c, t dbSNP:762452073
967 967 a, c, g dbSNP:528520765
968 968 c, g dbSNP:760951591
970 970 c, g dbSNP:775579171
973 973 a, g dbSNP:772133023
977 977 g, t dbSNP:559466164
986 986 c, g dbSNP:201365992
988 988 c, t dbSNP:770821731
989 989 c, g, t dbSNP:777610133
990 990 c, t dbSNP:755928135
995 995 a, g dbSNP:747764937
996 996 g, t dbSNP:780528085
997 997 c, t dbSNP:769931960
1003 1003 a, g dbSNP:75538505
1016 1016 a, g dbSNP:766038717
1025 1025 a, g dbSNP:563998083
1040 1040 a, g dbSNP:144363716
1046 1046 a, c dbSNP:200220557
1047 1047 a, g dbSNP:760962608
1050 1050 c, t dbSNP:575065229
1055 1055 a, g dbSNP:775733855
1058 1058 a, g dbSNP:767760072
1061 1061 c, t dbSNP:759792964
1062 1062 a, g dbSNP:376247953
1064 1064 c, t dbSNP:1057822
1067 1067 c, t dbSNP:771056515
1070 1070 a, c, t dbSNP:145743493
1086 1086 a, c dbSNP:769570694
1090 1090 a, g dbSNP:747914994
1094 1094 c, t dbSNP:780854735
1109 1109 c, t dbSNP:745954500
1120 1120 a, g dbSNP:754657721
1121 1121 c, t dbSNP:373054624
1124 1124 c, t dbSNP:779710286
1146 1146 c, t dbSNP:369251331
1151 1151 c, g dbSNP:754313230
1163 1163 g, t dbSNP:764531356
1166 1166 a, c dbSNP:376001204
1172 1172 c, t dbSNP:756463256
1174 1174 a, g dbSNP:752975669
1191 1191 a, g dbSNP:767916069
1199 1199 c, t dbSNP:139707934
1207 1207 a, g dbSNP:774622668
1208 1208 a, g dbSNP:766379743
1220 1220 c, t dbSNP:200166023
1226 1226 c, t dbSNP:773149231
1227 1227 a, g dbSNP:769647331
1234 1234 c, t dbSNP:541542280
1235 1235 c, t dbSNP:776556262
1239 1239 c, t dbSNP:768643446
1241 1241 a, g dbSNP:201220243
1250 1250 c, t dbSNP:541462988
1251 1251 a, g dbSNP:779865918
1253 1253 a, c dbSNP:771660894
1259 1259 c, t dbSNP:369149201
1260 1260 a, g, t dbSNP:371536347
1261 1261 a, g dbSNP:753303580
1271 1271 c, t dbSNP:143108243
1273 1273 a, g dbSNP:755340236
1276 1276 c, g dbSNP:751719158
1292 1292 c, t dbSNP:766607213
1295 1295 c, g dbSNP:763045676
1298 1298 c, t dbSNP:750435465
1316 1316 c, g dbSNP:765418238
1331 1331 c, t dbSNP:767401448
1345 1345 a, g dbSNP:560996946
1352 1352 a, g dbSNP:201523464
1353 1353 g, t dbSNP:774056264
1360 1360 a, t dbSNP:770288705
1362 1362 c, t dbSNP:748814440
1363 1363 a, g dbSNP:542924332
1367 1367 c, t dbSNP:777082260
1374 1374 a, c dbSNP:769118636
1378 1378 c, g dbSNP:80358193
1400 1400 a, g dbSNP:747561523
1403 1403 a, g dbSNP:374733352
1421 1421 c, t dbSNP:758796600
1427 1427 c, g dbSNP:746020646
1448 1448 a, t dbSNP:368903308
1458 1458 c, t dbSNP:752961498
1484 1484 a, g dbSNP:772277193
1493 1493 c, t dbSNP:746279366
1494 1494 a, g dbSNP:376342020
1504 1504 a, g dbSNP:146367075
1507 1507 c, t dbSNP:749582767
1511 1511 c, t dbSNP:534713982
1527 1527 -, t dbSNP:754085768
1540 1540 a, g dbSNP:119476044
1541 1541 c, t dbSNP:143991968
1547 1547 c, t dbSNP:756264599
1549 1549 a, g dbSNP:119476045
1578 1578 g, t dbSNP:773524524
1587 1587 g, t dbSNP:770109705
1588 1588 g, t dbSNP:137949094
1593 1593 a, g dbSNP:781317846
1594 1594 a, g dbSNP:768347513
1600 1600 c, t dbSNP:768683806
1604 1604 a, g dbSNP:748926816
1613 1613 c, t dbSNP:779990034
1619 1619 a, c, t dbSNP:147839743
1620 1620 a, g dbSNP:149179666
1623 1623 c, t dbSNP:756924512
1624 1624 a, g dbSNP:753505082
1625 1625 g, t dbSNP:763596301
1632 1632 c, t dbSNP:61734647
1635 1635 c, g dbSNP:752061561
1639 1639 g, t dbSNP:767020788
1654 1654 a, g dbSNP:763591260
1662 1662 c, g dbSNP:199612416
1664 1664 c, t dbSNP:765754091
1665 1665 a, c dbSNP:762088768
1670 1670 a, g dbSNP:200402260
1674 1674 -, acaa dbSNP:758224125
1677 1677 a, g dbSNP:768734691
1678 1678 c, t dbSNP:770063599
1680 1680 c, t dbSNP:747091749
1681 1681 c, t dbSNP:775588229
1689 1689 a, g dbSNP:369952046
1709 1709 g, t dbSNP:571961024
1784 1784 a, g dbSNP:55825888
1814 1814 g, t dbSNP:781574571
1872 1872 a, c dbSNP:552048121
1931 1931 a, c dbSNP:757801410
1953 1953 a, g dbSNP:183940450
1957 1957 c, t dbSNP:569310146
1990 1990 a, g dbSNP:533283653
1994 1994 c, t dbSNP:747131134
2021 2021 a, g dbSNP:549470454
2028 2028 a, g dbSNP:192117490
2057 2057 a, g dbSNP:560150547
2093 2093 a, g dbSNP:186972462
2095 2095 -, g dbSNP:373302655
2110 2110 c, g dbSNP:547609538
2135 2135 a, g dbSNP:144067360
2136 2136 a, g dbSNP:529087573
2165 2165 c, t dbSNP:564839436
2169 2169 a, g dbSNP:561398603
2204 2204 c, g dbSNP:755084960
2216 2216 a, t dbSNP:373743992
2229 2229 a, g dbSNP:755882450
2238 2238 c, g dbSNP:370227263
2263 2263 a, g dbSNP:183632912
2280 2280 -, t dbSNP:200787148
2286 2286 -, tttt dbSNP:781630598
2286 2286 -, t dbSNP:755626247
2289 2289 -, t dbSNP:149920332
2300 2300 a, g dbSNP:556055863
2304 2304 c, t dbSNP:375652885
2309 2309 a, c dbSNP:765174309
2341 2341 a, g dbSNP:146191273
2348 2348 g, t dbSNP:575617458
2404 2404 a, g dbSNP:190383315
2409 2409 -, gtttt dbSNP:576306593
2416 2416 -, t dbSNP:756990766
2422 2422 a, c dbSNP:563768747
2428 2428 g, t dbSNP:553844006
2449 2449 a, g dbSNP:753734936
2454 2454 -, aagagt dbSNP:758020025
2466 2466 a, g dbSNP:533764183
2489 2489 c, t dbSNP:185618019
2509 2509 a, c dbSNP:558495599
2513 2513 c, t dbSNP:767396009
2544 2544 a, c dbSNP:181172492
2576 2576 c, t dbSNP:761718628
2581 2581 a, g dbSNP:373111284
2606 2606 a, g dbSNP:773908732
2630 2630 c, t dbSNP:7550833
2632 2632 a, g dbSNP:762387297
2679 2679 -, t dbSNP:34699489
2729 2729 c, t dbSNP:763620691
2745 2745 c, t dbSNP:188357088
2765 2765 c, t dbSNP:374107175
2826 2826 c, t dbSNP:775022659
2840 2840 a, g dbSNP:185930120
2874 2874 a, g dbSNP:566624356
2877 2877 -, tg dbSNP:564384828
2900 2900 a, c dbSNP:746253526
2929 2929 c, t dbSNP:578226800
2930 2930 a, g dbSNP:771622080
2986 2986 a, g dbSNP:560764370
2998 2998 g, t dbSNP:747509481
3073 3073 -, ttc dbSNP:376965249
3075 3075 -, ctt dbSNP:201012894
3104 3104 a, t dbSNP:778353952
3128 3128 c, t dbSNP:574739784
3133 3133 a, g dbSNP:758564357
3158 3158 c, t dbSNP:368862498
3224 3224 c, t dbSNP:553402212
3225 3225 a, g dbSNP:533165406
3232 3232 a, g dbSNP:542483126
3249 3249 c, g dbSNP:182146084
3265 3265 a, c dbSNP:767096160
3295 3295 a, g dbSNP:748209019
3350 3350 a, g dbSNP:551676309
3355 3355 a, t dbSNP:531484702
3372 3372 c, t dbSNP:759117979
3390 3390 c, t dbSNP:562736932
3421 3421 a, g dbSNP:190617118
3425 3425 a, c dbSNP:755194746
3428 3428 g, t dbSNP:143135847
3437 3437 -, a, g dbSNP:3215625
3443 3443 -, a dbSNP:11386615
3444 3444 -, a, aaaaaaaa, gaaaaaaa dbSNP:541569859
3492 3492 c, g dbSNP:368824788
3495 3495 g, t dbSNP:774937283
3510 3510 a, g dbSNP:560290409
3521 3521 a, t dbSNP:13239
3531 3531 c, g dbSNP:139634340
3560 3560 c, t dbSNP:41303095
3606 3606 a, g dbSNP:185322052
3638 3638 a, g dbSNP:757252799
3680 3680 a, g dbSNP:538566469
3684 3684 c, t dbSNP:193073371
3686 3686 c, t dbSNP:150577852
3702 3702 c, g dbSNP:116262857
3712 3712 g, t dbSNP:566684988
3722 3722 a, t dbSNP:764841215
3737 3737 c, t dbSNP:141735901
3746 3746 c, g dbSNP:539519943
3762 3762 -, tttg dbSNP:143718156
3771 3771 a, c dbSNP:201470690
3793 3793 c, t dbSNP:759209798
3841 3841 -, a dbSNP:35245607
3849 3849 a, c dbSNP:570539035
3862 3862 a, c dbSNP:575966303
3876 3876 c, g dbSNP:191867
3892 3892 c, t dbSNP:770080187
3913 3913 a, g dbSNP:367869687
3923 3923 a, g dbSNP:113305916
4003 4003 a, g dbSNP:531672374
4033 4033 a, g dbSNP:570041962
4046 4046 c, t dbSNP:112836324
4057 4057 -, ttgt dbSNP:574848765
4104 4104 c, t dbSNP:549210917
4150 4150 c, t dbSNP:188644101
4239 4239 a, g dbSNP:77496832
4294 4294 a, c dbSNP:771422163

Target ORF information:

RefSeq Version XM_005273166
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens egl-9 family hypoxia-inducible factor 1 (EGLN1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005273166

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGCCAATG ACAGCGGCGG GCCCGGCGGG CCGAGCCCGA GCGAGCGAGA CCGGCAGTAC 
TGCGAGCTGT GCGGGAAGAT GGAGAACCTG CTGCGCTGCA GCCGCTGCCG CAGCTCCTTC
TACTGCTGCA AGGAGCACCA GCGTCAGGAC TGGAAGAAGC ACAAGCTCGT GTGCCAGGGC
AGCGAGGGCG CCCTCGGCCA CGGAGTGGGC CCACACCAGC ATTCCGGCCC CGCGCCGCCG
GCTGCAGTGC CGCCGCCCAG GGCCGGGGCC CGGGAGCCCA GGAAGGCAGC GGCGCGCCGG
GACAACGCCT CCGGGGACGC GGCCAAGGGA AAAGTAAAGG CCAAGCCCCC GGCCGACCCA
GCGGCGGCCG CGTCGCCGTG TCGTGCGGCC GCCGGCGGCC AGGGCTCGGC GGTGGCTGCC
GAAGCCGAGC CCGGCAAGGA GGAGCCGCCG GCCCGCTCAT CGCTGTTCCA GGAGAAGGCG
AACCTGTACC CCCCAAGCAA CACGCCCGGG GATGCGCTGA GCCCCGGCGG CGGCCTGCGG
CCCAACGGGC AGACGAAGCC CCTGCCGGCG CTGAAGCTGG CGCTCGAGTA CATCGTGCCG
TGCATGAACA AGCACGGCAT CTGTGTGGTG GACGACTTCC TCGGCAAGGA GACCGGACAG
CAGATCGGCG ACGAGGTGCG CGCCCTGCAC GACACCGGGA AGTTCACGGA CGGGCAGCTG
GTCAGCCAGA AGAGTGACTC GTCCAAGGAC ATCCGAGGCG ATAAGATCAC CTGGATCGAG
GGCAAGGAGC CCGGCTGCGA AACCATTGGG CTGCTCATGA GCAGCATGGA CGACCTGATA
CGCCACTGTA ACGGGAAGCT GGGCAGCTAC AAAATCAATG GCCGGACGAA AGCCATGGTT
GCTTGTTATC CGGGCAATGG AACGGGTTAT GTACGTCATG TTGATAATCC AAATGGAGAT
GGAAGATGTG TGACATGTAT ATATTATCTT AATAAAGACT GGGATGCCAA GGTAAGTGGA
GGTATACTTC GAATTTTTCC AGAAGGCAAA GCCCAGTTTG CTGACATTGA ACCCAAATTT
GATAGACTGC TGTTTTTCTG GTCTGACCGT CGCAACCCTC ATGAAGTACA ACCAGCATAT
GCTACAAGGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu35043
Accession Version XM_005273167.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1038bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product egl nine homolog 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167186.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578802251. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCCAATG ACAGCGGCGG GCCCGGCGGG CCGAGCCCGA GCGAGCGAGA CCGGCAGTAC 
TGCGAGCTGT GCGGGAAGAT GGAGAACCTG CTGCGCTGCA GCCGCTGCCG CAGCTCCTTC
TACTGCTGCA AGGAGCACCA GCGTCAGGAC TGGAAGAAGC ACAAGCTCGT GTGCCAGGGC
AGCGAGGGCG CCCTCGGCCA CGGAGTGGGC CCACACCAGC ATTCCGGCCC CGCGCCGCCG
GCTGCAGTGC CGCCGCCCAG GGCCGGGGCC CGGGAGCCCA GGAAGGCAGC GGCGCGCCGG
GACAACGCCT CCGGGGACGC GGCCAAGGGA AAAGTAAAGG CCAAGCCCCC GGCCGACCCA
GCGGCGGCCG CGTCGCCGTG TCGTGCGGCC GCCGGCGGCC AGGGCTCGGC GGTGGCTGCC
GAAGCCGAGC CCGGCAAGGA GGAGCCGCCG GCCCGCTCAT CGCTGTTCCA GGAGAAGGCG
AACCTGTACC CCCCAAGCAA CACGCCCGGG GATGCGCTGA GCCCCGGCGG CGGCCTGCGG
CCCAACGGGC AGACGAAGCC CCTGCCGGCG CTGAAGCTGG CGCTCGAGTA CATCGTGCCG
TGCATGAACA AGCACGGCAT CTGTGTGGTG GACGACTTCC TCGGCAAGGA GACCGGACAG
CAGATCGGCG ACGAGGTGCG CGCCCTGCAC GACACCGGGA AGTTCACGGA CGGGCAGCTG
GTCAGCCAGA AGAGTGACTC GTCCAAGGAC ATCCGAGGCG ATAAGATCAC CTGGATCGAG
GGCAAGGAGC CCGGCTGCGA AACCATTGGG CTGCTCATGA GCAGCATGGA CGACCTGATA
CGCCACTGTA ACGGGAAGCT GGGCAGCTAC AAAATCAATG GCCGGACGAA AGCCATGGTT
GCTTGTTATC CGGGCAATGG AACGGGTTAT GTACGTCATG TTGATAATCC AAATGGAGAT
GGAAGATGTG TGACATGTAT ATATTATCTT AATAAAGACT GGGATGCCAA GGTACGCAAT
AACTGTTTGG TATTTTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005273224.1
CDS429..1466
Misc Feature(1)489..602(+)
Misc Feature(2)1086..>1427(+)
Translation
Position Chain Variation Link
9 9 c, g dbSNP:533281866
50 50 a, c, g, t dbSNP:533584823
52 52 a, g dbSNP:564650969
55 55 c, t dbSNP:551144196
95 95 c, t dbSNP:531019844
96 96 a, g dbSNP:562125893
121 121 c, t dbSNP:542211420
183 183 c, t dbSNP:573128718
274 274 g, t dbSNP:12049545
368 368 a, g dbSNP:540141256
388 388 a, g dbSNP:772541408
389 389 c, t dbSNP:746436779
392 392 c, g dbSNP:779132675
403 403 c, t dbSNP:757708476
405 405 c, g dbSNP:577443284
406 406 c, t dbSNP:557526823
409 409 c, t dbSNP:756211725
414 414 -, tcgccg dbSNP:759405757
416 416 a, c, t dbSNP:767744500
418 418 c, t dbSNP:754940670
423 423 -, gccgcc dbSNP:776826207
428 428 -, gcc, gccgcc, gccgccgcc dbSNP:574288484
440 440 c, g dbSNP:186996510
452 452 c, t dbSNP:766080154
453 453 c, g dbSNP:762808532
455 455 c, t dbSNP:575002938
464 464 c, g dbSNP:764903617
469 469 a, g dbSNP:761497751
470 470 c, g dbSNP:776101026
495 495 c, t dbSNP:768214321
505 505 a, g dbSNP:746356152
507 507 a, t dbSNP:774928155
515 515 c, t dbSNP:771144835
524 524 c, t dbSNP:749684908
531 531 c, g dbSNP:778100040
548 548 c, t dbSNP:555121182
555 555 a, t dbSNP:748429073
559 559 a, g dbSNP:781405202
568 568 a, g dbSNP:73121547
574 574 a, g dbSNP:755147883
576 576 a, g dbSNP:751382793
583 583 a, g dbSNP:566308354
584 584 a, g dbSNP:766365190
594 594 c, t dbSNP:758090254
596 596 c, t dbSNP:750205790
597 597 a, g dbSNP:765070023
611 611 c, t dbSNP:761442296
618 618 a, g dbSNP:776333127
629 629 c, t dbSNP:377685277
630 630 c, g dbSNP:763530007
632 632 a, g dbSNP:760247413
637 637 a, g dbSNP:774670831
655 655 g, t dbSNP:367712964
673 673 c, t dbSNP:771498926
703 703 a, c dbSNP:553659934
708 708 a, g dbSNP:539743848
715 715 c, t dbSNP:113401862
739 739 c, t dbSNP:551207815
747 747 g, t dbSNP:531081279
808 808 c, g dbSNP:12097901
810 810 c, g dbSNP:756094298
824 824 c, t dbSNP:748371954
833 833 c, t dbSNP:781566823
847 847 c, t dbSNP:768557500
852 852 a, g dbSNP:747146652
859 859 c, t dbSNP:780040550
873 873 a, c dbSNP:374893731
877 877 c, t dbSNP:758337994
879 879 a, g dbSNP:750421640
881 881 c, g dbSNP:778676941
883 883 a, g dbSNP:757050785
891 891 c, t dbSNP:753433323
898 898 a, g dbSNP:748272071
899 899 a, c, g dbSNP:61750991
900 900 -, gag dbSNP:747045922
905 905 a, g dbSNP:766985056
911 911 c, t dbSNP:763502578
917 917 c, t dbSNP:773919305
919 919 c, t dbSNP:770213782
920 920 a, c, g dbSNP:776956500
921 921 a, c, g dbSNP:200502960
922 922 c, g dbSNP:780171999
931 931 c, t dbSNP:772111647
933 933 c, t dbSNP:745738740
935 935 c, g, t dbSNP:756999294
938 938 a, g dbSNP:753663555
943 943 c, t dbSNP:777466821
944 944 a, g dbSNP:755769412
950 950 a, c, t dbSNP:767045832
952 952 c, g dbSNP:759117988
953 953 c, t dbSNP:750900131
961 961 g, t dbSNP:765865681
966 966 c, t dbSNP:762452073
967 967 a, c, g dbSNP:528520765
968 968 c, g dbSNP:760951591
970 970 c, g dbSNP:775579171
973 973 a, g dbSNP:772133023
977 977 g, t dbSNP:559466164
986 986 c, g dbSNP:201365992
988 988 c, t dbSNP:770821731
989 989 c, g, t dbSNP:777610133
990 990 c, t dbSNP:755928135
995 995 a, g dbSNP:747764937
996 996 g, t dbSNP:780528085
997 997 c, t dbSNP:769931960
1003 1003 a, g dbSNP:75538505
1016 1016 a, g dbSNP:766038717
1025 1025 a, g dbSNP:563998083
1040 1040 a, g dbSNP:144363716
1046 1046 a, c dbSNP:200220557
1047 1047 a, g dbSNP:760962608
1050 1050 c, t dbSNP:575065229
1055 1055 a, g dbSNP:775733855
1058 1058 a, g dbSNP:767760072
1061 1061 c, t dbSNP:759792964
1062 1062 a, g dbSNP:376247953
1064 1064 c, t dbSNP:1057822
1067 1067 c, t dbSNP:771056515
1070 1070 a, c, t dbSNP:145743493
1086 1086 a, c dbSNP:769570694
1090 1090 a, g dbSNP:747914994
1094 1094 c, t dbSNP:780854735
1109 1109 c, t dbSNP:745954500
1120 1120 a, g dbSNP:754657721
1121 1121 c, t dbSNP:373054624
1124 1124 c, t dbSNP:779710286
1146 1146 c, t dbSNP:369251331
1151 1151 c, g dbSNP:754313230
1163 1163 g, t dbSNP:764531356
1166 1166 a, c dbSNP:376001204
1172 1172 c, t dbSNP:756463256
1174 1174 a, g dbSNP:752975669
1191 1191 a, g dbSNP:767916069
1199 1199 c, t dbSNP:139707934
1207 1207 a, g dbSNP:774622668
1208 1208 a, g dbSNP:766379743
1220 1220 c, t dbSNP:200166023
1226 1226 c, t dbSNP:773149231
1227 1227 a, g dbSNP:769647331
1234 1234 c, t dbSNP:541542280
1235 1235 c, t dbSNP:776556262
1239 1239 c, t dbSNP:768643446
1241 1241 a, g dbSNP:201220243
1250 1250 c, t dbSNP:541462988
1251 1251 a, g dbSNP:779865918
1253 1253 a, c dbSNP:771660894
1259 1259 c, t dbSNP:369149201
1260 1260 a, g, t dbSNP:371536347
1261 1261 a, g dbSNP:753303580
1271 1271 c, t dbSNP:143108243
1273 1273 a, g dbSNP:755340236
1276 1276 c, g dbSNP:751719158
1292 1292 c, t dbSNP:766607213
1295 1295 c, g dbSNP:763045676
1298 1298 c, t dbSNP:750435465
1316 1316 c, g dbSNP:765418238
1331 1331 c, t dbSNP:767401448
1345 1345 a, g dbSNP:560996946
1352 1352 a, g dbSNP:201523464
1353 1353 g, t dbSNP:774056264
1360 1360 a, t dbSNP:770288705
1362 1362 c, t dbSNP:748814440
1363 1363 a, g dbSNP:542924332
1367 1367 c, t dbSNP:777082260
1374 1374 a, c dbSNP:769118636
1378 1378 c, g dbSNP:80358193
1400 1400 a, g dbSNP:747561523
1403 1403 a, g dbSNP:374733352
1421 1421 c, t dbSNP:758796600
1427 1427 c, g dbSNP:746020646
1442 1442 a, g dbSNP:761336320
1447 1447 a, g dbSNP:775821997
1482 1482 a, g dbSNP:772604485
1484 1484 a, g dbSNP:760013506
1485 1485 a, c dbSNP:774766704
1492 1492 a, g dbSNP:771491532
1493 1493 g, t dbSNP:149479360
1500 1500 c, t dbSNP:778151367
1509 1509 g, t dbSNP:773524524
1518 1518 g, t dbSNP:770109705
1519 1519 g, t dbSNP:137949094
1524 1524 a, g dbSNP:781317846
1525 1525 a, g dbSNP:768347513
1531 1531 c, t dbSNP:768683806
1535 1535 a, g dbSNP:748926816
1544 1544 c, t dbSNP:779990034
1550 1550 a, c, t dbSNP:147839743
1551 1551 a, g dbSNP:149179666
1554 1554 c, t dbSNP:756924512
1555 1555 a, g dbSNP:753505082
1556 1556 g, t dbSNP:763596301
1563 1563 c, t dbSNP:61734647
1566 1566 c, g dbSNP:752061561
1570 1570 g, t dbSNP:767020788
1585 1585 a, g dbSNP:763591260
1593 1593 c, g dbSNP:199612416
1595 1595 c, t dbSNP:765754091
1596 1596 a, c dbSNP:762088768
1601 1601 a, g dbSNP:200402260
1605 1605 -, acaa dbSNP:758224125
1608 1608 a, g dbSNP:768734691
1609 1609 c, t dbSNP:770063599
1611 1611 c, t dbSNP:747091749
1612 1612 c, t dbSNP:775588229
1620 1620 a, g dbSNP:369952046
1640 1640 g, t dbSNP:571961024
1715 1715 a, g dbSNP:55825888
1745 1745 g, t dbSNP:781574571
1803 1803 a, c dbSNP:552048121
1862 1862 a, c dbSNP:757801410
1884 1884 a, g dbSNP:183940450
1888 1888 c, t dbSNP:569310146
1921 1921 a, g dbSNP:533283653
1925 1925 c, t dbSNP:747131134
1952 1952 a, g dbSNP:549470454
1959 1959 a, g dbSNP:192117490
1988 1988 a, g dbSNP:560150547
2024 2024 a, g dbSNP:186972462
2026 2026 -, g dbSNP:373302655
2041 2041 c, g dbSNP:547609538
2066 2066 a, g dbSNP:144067360
2067 2067 a, g dbSNP:529087573
2096 2096 c, t dbSNP:564839436
2100 2100 a, g dbSNP:561398603
2135 2135 c, g dbSNP:755084960
2147 2147 a, t dbSNP:373743992
2160 2160 a, g dbSNP:755882450
2169 2169 c, g dbSNP:370227263
2194 2194 a, g dbSNP:183632912
2211 2211 -, t dbSNP:200787148
2217 2217 -, tttt dbSNP:781630598
2217 2217 -, t dbSNP:755626247
2220 2220 -, t dbSNP:149920332
2231 2231 a, g dbSNP:556055863
2235 2235 c, t dbSNP:375652885
2240 2240 a, c dbSNP:765174309
2272 2272 a, g dbSNP:146191273
2279 2279 g, t dbSNP:575617458
2335 2335 a, g dbSNP:190383315
2340 2340 -, gtttt dbSNP:576306593
2347 2347 -, t dbSNP:756990766
2353 2353 a, c dbSNP:563768747
2359 2359 g, t dbSNP:553844006
2380 2380 a, g dbSNP:753734936
2385 2385 -, aagagt dbSNP:758020025
2397 2397 a, g dbSNP:533764183
2420 2420 c, t dbSNP:185618019
2440 2440 a, c dbSNP:558495599
2444 2444 c, t dbSNP:767396009
2475 2475 a, c dbSNP:181172492
2507 2507 c, t dbSNP:761718628
2512 2512 a, g dbSNP:373111284
2537 2537 a, g dbSNP:773908732
2561 2561 c, t dbSNP:7550833
2563 2563 a, g dbSNP:762387297
2610 2610 -, t dbSNP:34699489
2660 2660 c, t dbSNP:763620691
2676 2676 c, t dbSNP:188357088
2696 2696 c, t dbSNP:374107175
2757 2757 c, t dbSNP:775022659
2771 2771 a, g dbSNP:185930120
2805 2805 a, g dbSNP:566624356
2808 2808 -, tg dbSNP:564384828
2831 2831 a, c dbSNP:746253526
2860 2860 c, t dbSNP:578226800
2861 2861 a, g dbSNP:771622080
2917 2917 a, g dbSNP:560764370
2929 2929 g, t dbSNP:747509481
3004 3004 -, ttc dbSNP:376965249
3006 3006 -, ctt dbSNP:201012894
3035 3035 a, t dbSNP:778353952
3059 3059 c, t dbSNP:574739784
3064 3064 a, g dbSNP:758564357
3089 3089 c, t dbSNP:368862498
3155 3155 c, t dbSNP:553402212
3156 3156 a, g dbSNP:533165406
3163 3163 a, g dbSNP:542483126
3180 3180 c, g dbSNP:182146084
3196 3196 a, c dbSNP:767096160
3226 3226 a, g dbSNP:748209019
3281 3281 a, g dbSNP:551676309
3286 3286 a, t dbSNP:531484702
3303 3303 c, t dbSNP:759117979
3321 3321 c, t dbSNP:562736932
3352 3352 a, g dbSNP:190617118
3356 3356 a, c dbSNP:755194746
3359 3359 g, t dbSNP:143135847
3368 3368 -, a, g dbSNP:3215625
3374 3374 -, a dbSNP:11386615
3375 3375 -, a, aaaaaaaa, gaaaaaaa dbSNP:541569859
3423 3423 c, g dbSNP:368824788
3426 3426 g, t dbSNP:774937283
3441 3441 a, g dbSNP:560290409
3452 3452 a, t dbSNP:13239
3462 3462 c, g dbSNP:139634340
3491 3491 c, t dbSNP:41303095
3537 3537 a, g dbSNP:185322052
3569 3569 a, g dbSNP:757252799
3611 3611 a, g dbSNP:538566469
3615 3615 c, t dbSNP:193073371
3617 3617 c, t dbSNP:150577852
3633 3633 c, g dbSNP:116262857
3643 3643 g, t dbSNP:566684988
3653 3653 a, t dbSNP:764841215
3668 3668 c, t dbSNP:141735901
3677 3677 c, g dbSNP:539519943
3693 3693 -, tttg dbSNP:143718156
3702 3702 a, c dbSNP:201470690
3724 3724 c, t dbSNP:759209798
3772 3772 -, a dbSNP:35245607
3780 3780 a, c dbSNP:570539035
3793 3793 a, c dbSNP:575966303
3807 3807 c, g dbSNP:191867
3823 3823 c, t dbSNP:770080187
3844 3844 a, g dbSNP:367869687
3854 3854 a, g dbSNP:113305916
3934 3934 a, g dbSNP:531672374
3964 3964 a, g dbSNP:570041962
3977 3977 c, t dbSNP:112836324
3988 3988 -, ttgt dbSNP:574848765
4035 4035 c, t dbSNP:549210917
4081 4081 c, t dbSNP:188644101
4170 4170 a, g dbSNP:77496832
4225 4225 a, c dbSNP:771422163

Target ORF information:

RefSeq Version XM_005273167
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens egl-9 family hypoxia-inducible factor 1 (EGLN1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005273167

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCCAATG ACAGCGGCGG GCCCGGCGGG CCGAGCCCGA GCGAGCGAGA CCGGCAGTAC 
TGCGAGCTGT GCGGGAAGAT GGAGAACCTG CTGCGCTGCA GCCGCTGCCG CAGCTCCTTC
TACTGCTGCA AGGAGCACCA GCGTCAGGAC TGGAAGAAGC ACAAGCTCGT GTGCCAGGGC
AGCGAGGGCG CCCTCGGCCA CGGAGTGGGC CCACACCAGC ATTCCGGCCC CGCGCCGCCG
GCTGCAGTGC CGCCGCCCAG GGCCGGGGCC CGGGAGCCCA GGAAGGCAGC GGCGCGCCGG
GACAACGCCT CCGGGGACGC GGCCAAGGGA AAAGTAAAGG CCAAGCCCCC GGCCGACCCA
GCGGCGGCCG CGTCGCCGTG TCGTGCGGCC GCCGGCGGCC AGGGCTCGGC GGTGGCTGCC
GAAGCCGAGC CCGGCAAGGA GGAGCCGCCG GCCCGCTCAT CGCTGTTCCA GGAGAAGGCG
AACCTGTACC CCCCAAGCAA CACGCCCGGG GATGCGCTGA GCCCCGGCGG CGGCCTGCGG
CCCAACGGGC AGACGAAGCC CCTGCCGGCG CTGAAGCTGG CGCTCGAGTA CATCGTGCCG
TGCATGAACA AGCACGGCAT CTGTGTGGTG GACGACTTCC TCGGCAAGGA GACCGGACAG
CAGATCGGCG ACGAGGTGCG CGCCCTGCAC GACACCGGGA AGTTCACGGA CGGGCAGCTG
GTCAGCCAGA AGAGTGACTC GTCCAAGGAC ATCCGAGGCG ATAAGATCAC CTGGATCGAG
GGCAAGGAGC CCGGCTGCGA AACCATTGGG CTGCTCATGA GCAGCATGGA CGACCTGATA
CGCCACTGTA ACGGGAAGCT GGGCAGCTAC AAAATCAATG GCCGGACGAA AGCCATGGTT
GCTTGTTATC CGGGCAATGG AACGGGTTAT GTACGTCATG TTGATAATCC AAATGGAGAT
GGAAGATGTG TGACATGTAT ATATTATCTT AATAAAGACT GGGATGCCAA GGTACGCAAT
AACTGTTTGG TATTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22997
Accession Version NM_022051.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1281bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product egl nine homolog 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF229245.1, AL117352.12 and AI017372.1. This sequence is a reference standard in the RefSeqGene project. On May 15, 2009 this sequence version replaced gi:13489072. Summary: The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). [provided by RefSeq, Nov 2009]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF229245.1, AJ310543.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCCAATG ACAGCGGCGG GCCCGGCGGG CCGAGCCCGA GCGAGCGAGA CCGGCAGTAC 
TGCGAGCTGT GCGGGAAGAT GGAGAACCTG CTGCGCTGCA GCCGCTGCCG CAGCTCCTTC
TACTGCTGCA AGGAGCACCA GCGTCAGGAC TGGAAGAAGC ACAAGCTCGT GTGCCAGGGC
AGCGAGGGCG CCCTCGGCCA CGGAGTGGGC CCACACCAGC ATTCCGGCCC CGCGCCGCCG
GCTGCAGTGC CGCCGCCCAG GGCCGGGGCC CGGGAGCCCA GGAAGGCAGC GGCGCGCCGG
GACAACGCCT CCGGGGACGC GGCCAAGGGA AAAGTAAAGG CCAAGCCCCC GGCCGACCCA
GCGGCGGCCG CGTCGCCGTG TCGTGCGGCC GCCGGCGGCC AGGGCTCGGC GGTGGCTGCC
GAAGCCGAGC CCGGCAAGGA GGAGCCGCCG GCCCGCTCAT CGCTGTTCCA GGAGAAGGCG
AACCTGTACC CCCCAAGCAA CACGCCCGGG GATGCGCTGA GCCCCGGCGG CGGCCTGCGG
CCCAACGGGC AGACGAAGCC CCTGCCGGCG CTGAAGCTGG CGCTCGAGTA CATCGTGCCG
TGCATGAACA AGCACGGCAT CTGTGTGGTG GACGACTTCC TCGGCAAGGA GACCGGACAG
CAGATCGGCG ACGAGGTGCG CGCCCTGCAC GACACCGGGA AGTTCACGGA CGGGCAGCTG
GTCAGCCAGA AGAGTGACTC GTCCAAGGAC ATCCGAGGCG ATAAGATCAC CTGGATCGAG
GGCAAGGAGC CCGGCTGCGA AACCATTGGG CTGCTCATGA GCAGCATGGA CGACCTGATA
CGCCACTGTA ACGGGAAGCT GGGCAGCTAC AAAATCAATG GCCGGACGAA AGCCATGGTT
GCTTGTTATC CGGGCAATGG AACGGGTTAT GTACGTCATG TTGATAATCC AAATGGAGAT
GGAAGATGTG TGACATGTAT ATATTATCTT AATAAAGACT GGGATGCCAA GGTAAGTGGA
GGTATACTTC GAATTTTTCC AGAAGGCAAA GCCCAGTTTG CTGACATTGA ACCCAAATTT
GATAGACTGC TGTTTTTCTG GTCTGACCGT CGCAACCCTC ATGAAGTACA ACCAGCATAT
GCTACAAGGT ACGCAATAAC TGTTTGGTAT TTTGATGCAG ATGAGAGAGC ACGAGCTAAA
GTAAAATATC TAACAGGTGA AAAAGGTGTG AGGGTTGAAC TCAATAAACC TTCAGATTCG
GTCGGTAAAG ACGTCTTCTA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_071334.1
CDS3157..4437
Misc Feature(1)3031..3033(+)
Misc Feature(2)3160..3162(+)
Misc Feature(3)3172..3216(+)
Misc Feature(4)3217..3330(+)
Misc Feature(5)3529..3531(+)
Misc Feature(6)3757..3759(+)
Misc Feature(7)3778..3780(+)
Misc Feature(8)3814..4329(+)
Misc Feature(9)3877..3909(+)
Misc Feature(10)4060..4062(+)
Misc Feature(11)4123..4125(+)
Misc Feature(12)4132..4134(+)
Exon (1)1..4047
Gene:EGLN1
Gene Synonym:
Exon (2)4048..4167
Gene:EGLN1
Gene Synonym:
Exon (3)4168..4304
Gene:EGLN1
Gene Synonym:
Exon (4)4305..4372
Gene:EGLN1
Gene Synonym:
Exon (5)4373..7097
Gene:EGLN1
Gene Synonym:
Translation
Position Chain Variation Link
10 10 -, a dbSNP:397803034
15 15 -, a dbSNP:111995599
79 79 a, c dbSNP:778312678
107 107 g, t dbSNP:369610366
112 112 a, g dbSNP:758802437
130 130 c, t dbSNP:538933164
140 140 -, ctct dbSNP:533127616
158 158 c, g dbSNP:748651424
160 160 a, g dbSNP:183970490
165 165 a, g dbSNP:549987684
234 234 c, t dbSNP:1339894
270 270 a, g dbSNP:755076898
272 272 -, at dbSNP:754995326
308 308 c, t dbSNP:567274755
329 329 a, g dbSNP:180851300
336 336 a, g dbSNP:527333812
340 340 -, aata dbSNP:765518625
348 348 c, t dbSNP:564892845
407 407 c, t dbSNP:545320696
413 413 c, t dbSNP:531866773
422 422 a, g dbSNP:12094665
433 433 a, t dbSNP:545180346
448 448 c, t dbSNP:373140957
461 461 a, g dbSNP:147783454
468 468 -, cact dbSNP:577920148
491 491 a, t dbSNP:553989069
527 527 c, g dbSNP:766385981
543 543 c, t dbSNP:190531118
556 556 -, tttat dbSNP:540095357
558 558 c, t dbSNP:1361384
565 565 g, t dbSNP:1361383
571 571 c, t dbSNP:2153364
589 589 g, t dbSNP:185603039
590 590 c, t dbSNP:193048016
701 701 a, c, g dbSNP:541100189
707 707 g, t dbSNP:143021749
709 709 a, c dbSNP:114449367
738 738 c, g dbSNP:188721782
788 788 c, t dbSNP:149120358
808 808 c, g dbSNP:776305403
812 812 c, g dbSNP:145066596
816 816 c, g dbSNP:750246100
828 828 a, g dbSNP:71636390
858 858 c, t dbSNP:576315616
881 881 a, g dbSNP:531162628
909 909 g, t dbSNP:372348941
954 954 c, t dbSNP:184200552
1005 1005 c, g dbSNP:746697731
1158 1158 c, g dbSNP:368402713
1187 1187 a, g dbSNP:772976578
1219 1219 a, t dbSNP:112414693
1256 1256 a, t dbSNP:539183020
1268 1268 -, aat dbSNP:767155519
1316 1316 a, g dbSNP:565638198
1348 1348 a, c dbSNP:748537901
1367 1367 a, g dbSNP:779076327
1396 1396 c, t dbSNP:560714492
1408 1408 c, g dbSNP:540415895
1424 1424 c, t dbSNP:578041241
1451 1451 c, t dbSNP:7544596
1461 1461 a, g dbSNP:191690250
1469 1469 a, g dbSNP:749744627
1525 1525 -, ag dbSNP:145140638
1548 1548 a, g dbSNP:41303113
1565 1565 c, t dbSNP:12406290
1567 1567 c, t dbSNP:368110035
1581 1581 a, g dbSNP:373684198
1588 1588 -, ctta dbSNP:749160420
1620 1620 c, g dbSNP:536531125
1640 1640 a, g dbSNP:750706558
1670 1670 c, t dbSNP:573803226
1673 1673 a, g dbSNP:553925003
1722 1722 c, t dbSNP:533709398
1731 1731 c, t dbSNP:767725559
1776 1776 a, g dbSNP:571322622
1800 1800 c, g dbSNP:111387099
1801 1801 c, t dbSNP:551035044
1825 1825 a, g dbSNP:537464394
1830 1830 a, c dbSNP:113772572
1856 1856 a, t dbSNP:757355756
1861 1861 g, t dbSNP:568496391
1868 1868 -, aaat dbSNP:567666242
1899 1899 a, t dbSNP:60445751
1901 1901 -, ttaccat dbSNP:200197385
1904 1904 c, t dbSNP:752549410
1925 1925 a, g dbSNP:772649596
1928 1928 g, t dbSNP:149936012
1933 1933 a, c dbSNP:374347516
1945 1945 a, g dbSNP:41310557
1962 1962 a, g dbSNP:547178976
2064 2064 a, c dbSNP:368061513
2080 2080 -, a dbSNP:561182924
2089 2089 -, ct dbSNP:542437602
2106 2106 c, g dbSNP:139265156
2134 2134 c, t dbSNP:759550832
2136 2136 c, t dbSNP:530968830
2152 2152 c, t dbSNP:776677944
2172 2172 a, g dbSNP:564590591
2178 2178 c, g dbSNP:187007708
2188 2188 a, g dbSNP:766034321
2229 2229 a, t dbSNP:182333595
2238 2238 c, t dbSNP:562063028
2318 2318 c, t dbSNP:773064533
2320 2320 a, g dbSNP:375381135
2339 2339 g, t dbSNP:146578729
2371 2371 a, g dbSNP:190966487
2380 2380 c, g dbSNP:563733267
2458 2458 c, t dbSNP:111816383
2459 2459 a, g dbSNP:80010432
2478 2478 c, t dbSNP:771116014
2486 2486 c, g dbSNP:749548516
2538 2538 c, g dbSNP:114235192
2605 2605 a, g dbSNP:577015305
2613 2613 c, t dbSNP:188879504
2617 2617 a, g dbSNP:557463888
2621 2621 c, t dbSNP:184124593
2626 2626 a, c, g dbSNP:113172460
2737 2737 c, g dbSNP:533281866
2778 2778 a, c, g, t dbSNP:533584823
2780 2780 a, g dbSNP:564650969
2783 2783 c, t dbSNP:551144196
2823 2823 c, t dbSNP:531019844
2824 2824 a, g dbSNP:562125893
2849 2849 c, t dbSNP:542211420
2911 2911 c, t dbSNP:573128718
3002 3002 g, t dbSNP:12049545
3096 3096 a, g dbSNP:540141256
3116 3116 a, g dbSNP:772541408
3117 3117 c, t dbSNP:746436779
3120 3120 c, g dbSNP:779132675
3131 3131 c, t dbSNP:757708476
3133 3133 c, g dbSNP:577443284
3134 3134 c, t dbSNP:557526823
3137 3137 c, t dbSNP:756211725
3142 3142 -, tcgccg dbSNP:759405757
3144 3144 a, c, t dbSNP:767744500
3146 3146 c, t dbSNP:754940670
3151 3151 -, gccgcc dbSNP:776826207
3156 3156 -, gcc, gccgcc, gccgccgcc dbSNP:574288484
3168 3168 c, g dbSNP:186996510
3180 3180 c, t dbSNP:766080154
3181 3181 c, g dbSNP:762808532
3183 3183 c, t dbSNP:575002938
3192 3192 c, g dbSNP:764903617
3197 3197 a, g dbSNP:761497751
3198 3198 c, g dbSNP:776101026
3223 3223 c, t dbSNP:768214321
3233 3233 a, g dbSNP:746356152
3235 3235 a, t dbSNP:774928155
3243 3243 c, t dbSNP:771144835
3252 3252 c, t dbSNP:749684908
3259 3259 c, g dbSNP:778100040
3276 3276 c, t dbSNP:555121182
3283 3283 a, t dbSNP:748429073
3287 3287 a, g dbSNP:781405202
3296 3296 a, g dbSNP:73121547
3302 3302 a, g dbSNP:755147883
3304 3304 a, g dbSNP:751382793
3311 3311 a, g dbSNP:566308354
3312 3312 a, g dbSNP:766365190
3322 3322 c, t dbSNP:758090254
3324 3324 c, t dbSNP:750205790
3325 3325 a, g dbSNP:765070023
3339 3339 c, t dbSNP:761442296
3346 3346 a, g dbSNP:776333127
3357 3357 c, t dbSNP:377685277
3358 3358 c, g dbSNP:763530007
3360 3360 a, g dbSNP:760247413
3365 3365 a, g dbSNP:774670831
3383 3383 g, t dbSNP:367712964
3401 3401 c, t dbSNP:771498926
3431 3431 a, c dbSNP:553659934
3436 3436 a, g dbSNP:539743848
3443 3443 c, t dbSNP:113401862
3467 3467 c, t dbSNP:551207815
3475 3475 g, t dbSNP:531081279
3536 3536 c, g dbSNP:12097901
3538 3538 c, g dbSNP:756094298
3552 3552 c, t dbSNP:748371954
3561 3561 c, t dbSNP:781566823
3575 3575 c, t dbSNP:768557500
3580 3580 a, g dbSNP:747146652
3587 3587 c, t dbSNP:780040550
3601 3601 a, c dbSNP:374893731
3605 3605 c, t dbSNP:758337994
3607 3607 a, g dbSNP:750421640
3609 3609 c, g dbSNP:778676941
3611 3611 a, g dbSNP:757050785
3619 3619 c, t dbSNP:753433323
3626 3626 a, g dbSNP:748272071
3627 3627 a, c, g dbSNP:61750991
3628 3628 -, gag dbSNP:747045922
3633 3633 a, g dbSNP:766985056
3639 3639 c, t dbSNP:763502578
3645 3645 c, t dbSNP:773919305
3647 3647 c, t dbSNP:770213782
3648 3648 a, c, g dbSNP:776956500
3649 3649 a, c, g dbSNP:200502960
3650 3650 c, g dbSNP:780171999
3659 3659 c, t dbSNP:772111647
3661 3661 c, t dbSNP:745738740
3663 3663 c, g, t dbSNP:756999294
3666 3666 a, g dbSNP:753663555
3671 3671 c, t dbSNP:777466821
3672 3672 a, g dbSNP:755769412
3678 3678 a, c, t dbSNP:767045832
3680 3680 c, g dbSNP:759117988
3681 3681 c, t dbSNP:750900131
3689 3689 g, t dbSNP:765865681
3694 3694 c, t dbSNP:762452073
3695 3695 a, c, g dbSNP:528520765
3696 3696 c, g dbSNP:760951591
3698 3698 c, g dbSNP:775579171
3701 3701 a, g dbSNP:772133023
3705 3705 g, t dbSNP:559466164
3714 3714 c, g dbSNP:201365992
3716 3716 c, t dbSNP:770821731
3717 3717 c, g, t dbSNP:777610133
3718 3718 c, t dbSNP:755928135
3723 3723 a, g dbSNP:747764937
3724 3724 g, t dbSNP:780528085
3725 3725 c, t dbSNP:769931960
3731 3731 a, g dbSNP:75538505
3744 3744 a, g dbSNP:766038717
3753 3753 a, g dbSNP:563998083
3768 3768 a, g dbSNP:144363716
3774 3774 a, c dbSNP:200220557
3775 3775 a, g dbSNP:760962608
3778 3778 c, t dbSNP:575065229
3783 3783 a, g dbSNP:775733855
3786 3786 a, g dbSNP:767760072
3789 3789 c, t dbSNP:759792964
3790 3790 a, g dbSNP:376247953
3792 3792 c, t dbSNP:1057822
3795 3795 c, t dbSNP:771056515
3798 3798 a, c, t dbSNP:145743493
3814 3814 a, c dbSNP:769570694
3818 3818 a, g dbSNP:747914994
3822 3822 c, t dbSNP:780854735
3837 3837 c, t dbSNP:745954500
3848 3848 a, g dbSNP:754657721
3849 3849 c, t dbSNP:373054624
3852 3852 c, t dbSNP:779710286
3874 3874 c, t dbSNP:369251331
3879 3879 c, g dbSNP:754313230
3891 3891 g, t dbSNP:764531356
3894 3894 a, c dbSNP:376001204
3900 3900 c, t dbSNP:756463256
3902 3902 a, g dbSNP:752975669
3919 3919 a, g dbSNP:767916069
3927 3927 c, t dbSNP:139707934
3935 3935 a, g dbSNP:774622668
3936 3936 a, g dbSNP:766379743
3948 3948 c, t dbSNP:200166023
3954 3954 c, t dbSNP:773149231
3955 3955 a, g dbSNP:769647331
3962 3962 c, t dbSNP:541542280
3963 3963 c, t dbSNP:776556262
3967 3967 c, t dbSNP:768643446
3969 3969 a, g dbSNP:201220243
3978 3978 c, t dbSNP:541462988
3979 3979 a, g dbSNP:779865918
3981 3981 a, c dbSNP:771660894
3987 3987 c, t dbSNP:369149201
3988 3988 a, g, t dbSNP:371536347
3989 3989 a, g dbSNP:753303580
3999 3999 c, t dbSNP:143108243
4001 4001 a, g dbSNP:755340236
4004 4004 c, g dbSNP:751719158
4020 4020 c, t dbSNP:766607213
4023 4023 c, g dbSNP:763045676
4026 4026 c, t dbSNP:750435465
4044 4044 c, g dbSNP:765418238
4059 4059 c, t dbSNP:767401448
4073 4073 a, g dbSNP:560996946
4080 4080 a, g dbSNP:201523464
4081 4081 g, t dbSNP:774056264
4088 4088 a, t dbSNP:770288705
4090 4090 c, t dbSNP:748814440
4091 4091 a, g dbSNP:542924332
4095 4095 c, t dbSNP:777082260
4102 4102 a, c dbSNP:769118636
4106 4106 c, g dbSNP:80358193
4128 4128 a, g dbSNP:747561523
4131 4131 a, g dbSNP:374733352
4149 4149 c, t dbSNP:758796600
4155 4155 c, g dbSNP:746020646
4176 4176 a, t dbSNP:368903308
4186 4186 c, t dbSNP:752961498
4212 4212 a, g dbSNP:772277193
4221 4221 c, t dbSNP:746279366
4222 4222 a, g dbSNP:376342020
4232 4232 a, g dbSNP:146367075
4235 4235 c, t dbSNP:749582767
4239 4239 c, t dbSNP:534713982
4255 4255 -, t dbSNP:754085768
4268 4268 a, g dbSNP:119476044
4269 4269 c, t dbSNP:143991968
4275 4275 c, t dbSNP:756264599
4277 4277 a, g dbSNP:119476045
4307 4307 a, g dbSNP:761336320
4312 4312 a, g dbSNP:775821997
4347 4347 a, g dbSNP:772604485
4349 4349 a, g dbSNP:760013506
4350 4350 a, c dbSNP:774766704
4357 4357 a, g dbSNP:771491532
4358 4358 g, t dbSNP:149479360
4365 4365 c, t dbSNP:778151367
4374 4374 g, t dbSNP:773524524
4383 4383 g, t dbSNP:770109705
4384 4384 g, t dbSNP:137949094
4389 4389 a, g dbSNP:781317846
4390 4390 a, g dbSNP:768347513
4396 4396 c, t dbSNP:768683806
4400 4400 a, g dbSNP:748926816
4409 4409 c, t dbSNP:779990034
4415 4415 a, c, t dbSNP:147839743
4416 4416 a, g dbSNP:149179666
4419 4419 c, t dbSNP:756924512
4420 4420 a, g dbSNP:753505082
4421 4421 g, t dbSNP:763596301
4428 4428 c, t dbSNP:61734647
4431 4431 c, g dbSNP:752061561
4435 4435 g, t dbSNP:767020788
4450 4450 a, g dbSNP:763591260
4458 4458 c, g dbSNP:199612416
4460 4460 c, t dbSNP:765754091
4461 4461 a, c dbSNP:762088768
4466 4466 a, g dbSNP:200402260
4470 4470 -, acaa dbSNP:758224125
4473 4473 a, g dbSNP:768734691
4474 4474 c, t dbSNP:770063599
4476 4476 c, t dbSNP:747091749
4477 4477 c, t dbSNP:775588229
4485 4485 a, g dbSNP:369952046
4505 4505 g, t dbSNP:571961024
4580 4580 a, g dbSNP:55825888
4610 4610 g, t dbSNP:781574571
4668 4668 a, c dbSNP:552048121
4727 4727 a, c dbSNP:757801410
4749 4749 a, g dbSNP:183940450
4753 4753 c, t dbSNP:569310146
4786 4786 a, g dbSNP:533283653
4790 4790 c, t dbSNP:747131134
4817 4817 a, g dbSNP:549470454
4824 4824 a, g dbSNP:192117490
4853 4853 a, g dbSNP:560150547
4889 4889 a, g dbSNP:186972462
4891 4891 -, g dbSNP:373302655
4906 4906 c, g dbSNP:547609538
4931 4931 a, g dbSNP:144067360
4932 4932 a, g dbSNP:529087573
4961 4961 c, t dbSNP:564839436
4965 4965 a, g dbSNP:561398603
5000 5000 c, g dbSNP:755084960
5012 5012 a, t dbSNP:373743992
5025 5025 a, g dbSNP:755882450
5034 5034 c, g dbSNP:370227263
5059 5059 a, g dbSNP:183632912
5076 5076 -, t dbSNP:200787148
5082 5082 -, tttt dbSNP:781630598
5082 5082 -, t dbSNP:755626247
5085 5085 -, t dbSNP:149920332
5096 5096 a, g dbSNP:556055863
5100 5100 c, t dbSNP:375652885
5105 5105 a, c dbSNP:765174309
5137 5137 a, g dbSNP:146191273
5144 5144 g, t dbSNP:575617458
5200 5200 a, g dbSNP:190383315
5205 5205 -, gtttt dbSNP:576306593
5212 5212 -, t dbSNP:756990766
5218 5218 a, c dbSNP:563768747
5224 5224 g, t dbSNP:553844006
5245 5245 a, g dbSNP:753734936
5250 5250 -, aagagt dbSNP:758020025
5262 5262 a, g dbSNP:533764183
5285 5285 c, t dbSNP:185618019
5305 5305 a, c dbSNP:558495599
5309 5309 c, t dbSNP:767396009
5340 5340 a, c dbSNP:181172492
5372 5372 c, t dbSNP:761718628
5377 5377 a, g dbSNP:373111284
5402 5402 a, g dbSNP:773908732
5426 5426 c, t dbSNP:7550833
5428 5428 a, g dbSNP:762387297
5475 5475 -, t dbSNP:34699489
5525 5525 c, t dbSNP:763620691
5541 5541 c, t dbSNP:188357088
5561 5561 c, t dbSNP:374107175
5622 5622 c, t dbSNP:775022659
5636 5636 a, g dbSNP:185930120
5670 5670 a, g dbSNP:566624356
5673 5673 -, tg dbSNP:564384828
5696 5696 a, c dbSNP:746253526
5725 5725 c, t dbSNP:578226800
5726 5726 a, g dbSNP:771622080
5782 5782 a, g dbSNP:560764370
5794 5794 g, t dbSNP:747509481
5869 5869 -, ttc dbSNP:376965249
5871 5871 -, ctt dbSNP:201012894
5900 5900 a, t dbSNP:778353952
5924 5924 c, t dbSNP:574739784
5929 5929 a, g dbSNP:758564357
5954 5954 c, t dbSNP:368862498
6020 6020 c, t dbSNP:553402212
6021 6021 a, g dbSNP:533165406
6028 6028 a, g dbSNP:542483126
6045 6045 c, g dbSNP:182146084
6061 6061 a, c dbSNP:767096160
6091 6091 a, g dbSNP:748209019
6146 6146 a, g dbSNP:551676309
6151 6151 a, t dbSNP:531484702
6168 6168 c, t dbSNP:759117979
6186 6186 c, t dbSNP:562736932
6217 6217 a, g dbSNP:190617118
6221 6221 a, c dbSNP:755194746
6224 6224 g, t dbSNP:143135847
6233 6233 -, a, g dbSNP:3215625
6239 6239 -, a dbSNP:11386615
6240 6240 -, a, aaaaaaaa, gaaaaaaa dbSNP:541569859
6288 6288 c, g dbSNP:368824788
6291 6291 g, t dbSNP:774937283
6306 6306 a, g dbSNP:560290409
6317 6317 a, t dbSNP:13239
6327 6327 c, g dbSNP:139634340
6356 6356 c, t dbSNP:41303095
6402 6402 a, g dbSNP:185322052
6434 6434 a, g dbSNP:757252799
6476 6476 a, g dbSNP:538566469
6480 6480 c, t dbSNP:193073371
6482 6482 c, t dbSNP:150577852
6498 6498 c, g dbSNP:116262857
6508 6508 g, t dbSNP:566684988
6518 6518 a, t dbSNP:764841215
6533 6533 c, t dbSNP:141735901
6542 6542 c, g dbSNP:539519943
6558 6558 -, tttg dbSNP:143718156
6567 6567 a, c dbSNP:201470690
6589 6589 c, t dbSNP:759209798
6637 6637 -, a dbSNP:35245607
6645 6645 a, c dbSNP:570539035
6658 6658 a, c dbSNP:575966303
6672 6672 c, g dbSNP:191867
6688 6688 c, t dbSNP:770080187
6709 6709 a, g dbSNP:367869687
6719 6719 a, g dbSNP:113305916
6799 6799 a, g dbSNP:531672374
6829 6829 a, g dbSNP:570041962
6842 6842 c, t dbSNP:112836324
6853 6853 -, ttgt dbSNP:574848765
6900 6900 c, t dbSNP:549210917
6946 6946 c, t dbSNP:188644101
7035 7035 a, g dbSNP:77496832
7090 7090 a, c dbSNP:771422163

Target ORF information:

RefSeq Version NM_022051
Organism Homo sapiens (human)
Definition Homo sapiens egl-9 family hypoxia-inducible factor 1 (EGLN1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_022051

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCCAATG ACAGCGGCGG GCCCGGCGGG CCGAGCCCGA GCGAGCGAGA CCGGCAGTAC 
TGCGAGCTGT GCGGGAAGAT GGAGAACCTG CTGCGCTGCA GCCGCTGCCG CAGCTCCTTC
TACTGCTGCA AGGAGCACCA GCGTCAGGAC TGGAAGAAGC ACAAGCTCGT GTGCCAGGGC
AGCGAGGGCG CCCTCGGCCA CGGAGTGGGC CCACACCAGC ATTCCGGCCC CGCGCCGCCG
GCTGCAGTGC CGCCGCCCAG GGCCGGGGCC CGGGAGCCCA GGAAGGCAGC GGCGCGCCGG
GACAACGCCT CCGGGGACGC GGCCAAGGGA AAAGTAAAGG CCAAGCCCCC GGCCGACCCA
GCGGCGGCCG CGTCGCCGTG TCGTGCGGCC GCCGGCGGCC AGGGCTCGGC GGTGGCTGCC
GAAGCCGAGC CCGGCAAGGA GGAGCCGCCG GCCCGCTCAT CGCTGTTCCA GGAGAAGGCG
AACCTGTACC CCCCAAGCAA CACGCCCGGG GATGCGCTGA GCCCCGGCGG CGGCCTGCGG
CCCAACGGGC AGACGAAGCC CCTGCCGGCG CTGAAGCTGG CGCTCGAGTA CATCGTGCCG
TGCATGAACA AGCACGGCAT CTGTGTGGTG GACGACTTCC TCGGCAAGGA GACCGGACAG
CAGATCGGCG ACGAGGTGCG CGCCCTGCAC GACACCGGGA AGTTCACGGA CGGGCAGCTG
GTCAGCCAGA AGAGTGACTC GTCCAAGGAC ATCCGAGGCG ATAAGATCAC CTGGATCGAG
GGCAAGGAGC CCGGCTGCGA AACCATTGGG CTGCTCATGA GCAGCATGGA CGACCTGATA
CGCCACTGTA ACGGGAAGCT GGGCAGCTAC AAAATCAATG GCCGGACGAA AGCCATGGTT
GCTTGTTATC CGGGCAATGG AACGGGTTAT GTACGTCATG TTGATAATCC AAATGGAGAT
GGAAGATGTG TGACATGTAT ATATTATCTT AATAAAGACT GGGATGCCAA GGTAAGTGGA
GGTATACTTC GAATTTTTCC AGAAGGCAAA GCCCAGTTTG CTGACATTGA ACCCAAATTT
GATAGACTGC TGTTTTTCTG GTCTGACCGT CGCAACCCTC ATGAAGTACA ACCAGCATAT
GCTACAAGGT ACGCAATAAC TGTTTGGTAT TTTGATGCAG ATGAGAGAGC ACGAGCTAAA
GTAAAATATC TAACAGGTGA AAAAGGTGTG AGGGTTGAAC TCAATAAACC TTCAGATTCG
GTCGGTAAAG ACGTCTTCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.