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POU5F1 POU class 5 homeobox 1 [Homo sapiens (human)]

Gene Symbol POU5F1
Entrez Gene ID 5460
Full Name POU class 5 homeobox 1
Synonyms OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4
General protein information
Preferred Names
POU domain, class 5, transcription factor 1
Names
POU domain, class 5, transcription factor 1
octamer-binding protein 3
octamer-binding protein 4
POU domain transcription factor OCT4
octamer-binding transcription factor 3
octamer-binding transcription factor-3
POU-type homeodomain-containing DNA-binding protein
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p21.31

Summary This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]. lac of sum
Disorder MIM:

164177

Disorder Html:





The following POU5F1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POU5F1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu20662 NM_001173531 Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $99
OHu21050 NM_001285987 Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu20361 NM_002701 Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$379
OHu21117 NM_001285986 Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99
OHu20662 NM_203289 Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $269

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu20662
Accession Version NM_001173531.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 573bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 10-AUG-2015
Organism Homo sapiens (human)
Product POU domain, class 5, transcription factor 1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ486514.1, DQ486516.1, DQ486515.1 and AI811039.1. On Oct 26, 2013 this sequence version replaced gi:291167756. Summary: This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame non-AUG (CUG) start codon, compared to variant 1. The resulting isoform (2, also known as OCT4B-190) is shorter at the N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2). This variant may encode an additional isoform through the use of an alternative downstream AUG start codon. Use of alternate start codons and the non-AUG start codon is described in PMID:19489092. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ486516.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2150385, SAMEA2157511 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 19489092 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
CTGGGGGTTC TATTTGGGAA GGTATTCAGC CAAACGACCA TCTGCCGCTT TGAGGCTCTG 
CAGCTTAGCT TCAAGAACAT GTGTAAGCTG CGGCCCTTGC TGCAGAAGTG GGTGGAGGAA
GCTGACAACA ATGAAAATCT TCAGGAGATA TGCAAAGCAG AAACCCTCGT GCAGGCCCGA
AAGAGAAAGC GAACCAGTAT CGAGAACCGA GTGAGAGGCA ACCTGGAGAA TTTGTTCCTG
CAGTGCCCGA AACCCACACT GCAGCAGATC AGCCACATCG CCCAGCAGCT TGGGCTCGAG
AAGGATGTGG TCCGAGTGTG GTTCTGTAAC CGGCGCCAGA AGGGCAAGCG ATCAAGCAGC
GACTATGCAC AACGAGAGGA TTTTGAGGCT GCTGGGTCTC CTTTCTCAGG GGGACCAGTG
TCCTTTCCTC TGGCCCCAGG GCCCCATTTT GGTACCCCAG GCTATGGGAG CCCTCACTTC
ACTGCACTGT ACTCCTCGGT CCCTTTCCCT GAGGGGGAAG CCTTTCCCCC TGTCTCCGTC
ACCACTCTGG GCTCTCCCAT GCATTCAAAC TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001167002.1
CDS743..1315
Misc Feature(1)623..625(+)
Misc Feature(2)743..868(+)
Misc Feature(3)821..823(+)
Misc Feature(4)923..1099(+)
Misc Feature(5)923..1093(+)
Misc Feature(6)929..1081(+)
Exon (1)1..637
Gene:POU5F1
Gene Synonym:
Exon (2)638..758
Gene:POU5F1
Gene Synonym:
Exon (3)759..889
Gene:POU5F1
Gene Synonym:
Exon (4)890..1048
Gene:POU5F1
Gene Synonym:
Exon (5)1049..1579
Gene:POU5F1
Gene Synonym:
Translation
Position Chain Variation Link
1 1 a, g dbSNP:756575303
15 15 c, t dbSNP:556183524
38 38 a, g dbSNP:767061672
60 60 a, g dbSNP:3130931
91 91 a, g dbSNP:750002991
106 106 a, t dbSNP:573550776
115 115 a, g dbSNP:76465289
135 135 c, g dbSNP:536581051
151 151 g, t dbSNP:769146675
162 162 a, g dbSNP:566021008
179 179 a, g dbSNP:749630858
206 206 a, g dbSNP:776087600
221 221 a, g dbSNP:200340326
223 223 -, gag dbSNP:9281235
223 223 g, t dbSNP:77085553
224 224 -, gag dbSNP:72545985
225 225 -, gag, tga dbSNP:67207605
226 226 -, aag, gag dbSNP:141270342
227 227 g, t dbSNP:201576321
229 229 a, c dbSNP:553951373
283 283 -, gt dbSNP:571828146
323 323 c, t dbSNP:72856737
331 331 a, c dbSNP:571668450
333 333 c, g dbSNP:759635638
348 348 a, t dbSNP:545437201
349 349 c, t dbSNP:9263800
357 357 a, g dbSNP:11965454
407 407 c, g dbSNP:567697919
416 416 c, t dbSNP:3757349
450 450 a, t dbSNP:775390135
466 466 g, t dbSNP:73401031
496 496 -, cttt dbSNP:550996676
497 497 -, t dbSNP:533341709
507 507 -, tt dbSNP:67692102
507 507 -, t dbSNP:764189579
508 508 c, t dbSNP:201876558
508 508 -, t dbSNP:9279005
508 508 -, t dbSNP:757541562
509 509 c, t dbSNP:200941459
520 520 -, ag dbSNP:776463063
546 546 a, g dbSNP:574609806
587 587 a, c dbSNP:141017974
625 625 a, g dbSNP:151095308
639 639 a, c dbSNP:768359010
649 649 c, t dbSNP:749071230
654 654 c, t dbSNP:149025884
657 657 c, t dbSNP:137936040
670 670 c, t dbSNP:751745427
671 671 a, g dbSNP:777993058
676 676 a, g dbSNP:150320288
736 736 a, g dbSNP:752871883
760 760 a, g dbSNP:754269967
778 778 a, g dbSNP:373808092
816 816 a, g dbSNP:756413624
832 832 g, t dbSNP:115234511
833 833 c, t dbSNP:768029325
878 878 a, t dbSNP:572480837
887 887 a, g dbSNP:773889469
889 889 a, g dbSNP:763710527
908 908 c, t dbSNP:1150767
910 910 c, t dbSNP:780201728
911 911 a, c, g dbSNP:750755680
916 916 a, g dbSNP:767958442
943 943 c, t dbSNP:757769384
951 951 a, g dbSNP:751096375
952 952 a, t dbSNP:368434272
961 961 c, t dbSNP:578180451
976 976 g, t dbSNP:775301501
991 991 a, g dbSNP:764939136
997 997 c, g dbSNP:17851818
1003 1003 a, g dbSNP:776478147
1014 1014 a, g dbSNP:770844532
1021 1021 c, t dbSNP:746974556
1039 1039 c, t dbSNP:772966815
1040 1040 a, g dbSNP:768746587
1043 1043 a, g dbSNP:749326531
1050 1050 g, t dbSNP:770067555
1056 1056 a, g dbSNP:746104715
1066 1066 c, t dbSNP:781212227
1100 1100 a, g dbSNP:771345851
1102 1102 c, t dbSNP:141766253
1103 1103 a, g dbSNP:778460021
1110 1110 c, t dbSNP:546320542
1113 1113 -, aac dbSNP:765569430
1127 1127 a, g dbSNP:758948344
1140 1140 c, t dbSNP:752325450
1151 1151 a, g dbSNP:778574549
1183 1183 a, g dbSNP:754481951
1198 1198 c, g dbSNP:753553081
1205 1205 g, t dbSNP:765910375
1233 1233 a, c dbSNP:760558360
1239 1239 c, t dbSNP:750163518
1240 1240 a, g dbSNP:767500519
1252 1252 g, t dbSNP:761734933
1270 1270 a, c dbSNP:774206292
1271 1271 c, t dbSNP:769875118
1279 1279 c, t dbSNP:1061118
1284 1284 c, t dbSNP:1061120
1287 1287 c, g dbSNP:776940833
1294 1294 c, t dbSNP:771024256
1302 1302 a, t dbSNP:747446765
1326 1326 c, t dbSNP:564148752
1344 1344 -, a dbSNP:759833918
1344 1344 a, g dbSNP:772503540
1346 1346 a, g dbSNP:748680648
1356 1356 a, g dbSNP:779517517
1359 1359 a, g dbSNP:754549702
1371 1371 a, g dbSNP:1061126
1390 1390 a, g dbSNP:3734864
1441 1441 -, aa dbSNP:60004964
1441 1441 a, c dbSNP:200275741
1462 1462 -, t dbSNP:746543478
1517 1517 c, t dbSNP:537327593
1551 1551 -, gcc dbSNP:750617181
1553 1553 c, t dbSNP:13409

Target ORF information:

RefSeq Version NM_001173531
Organism Homo sapiens (human)
Definition Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001173531

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
CTGGGGGTTC TATTTGGGAA GGTATTCAGC CAAACGACCA TCTGCCGCTT TGAGGCTCTG 
CAGCTTAGCT TCAAGAACAT GTGTAAGCTG CGGCCCTTGC TGCAGAAGTG GGTGGAGGAA
GCTGACAACA ATGAAAATCT TCAGGAGATA TGCAAAGCAG AAACCCTCGT GCAGGCCCGA
AAGAGAAAGC GAACCAGTAT CGAGAACCGA GTGAGAGGCA ACCTGGAGAA TTTGTTCCTG
CAGTGCCCGA AACCCACACT GCAGCAGATC AGCCACATCG CCCAGCAGCT TGGGCTCGAG
AAGGATGTGG TCCGAGTGTG GTTCTGTAAC CGGCGCCAGA AGGGCAAGCG ATCAAGCAGC
GACTATGCAC AACGAGAGGA TTTTGAGGCT GCTGGGTCTC CTTTCTCAGG GGGACCAGTG
TCCTTTCCTC TGGCCCCAGG GCCCCATTTT GGTACCCCAG GCTATGGGAG CCCTCACTTC
ACTGCACTGT ACTCCTCGGT CCCTTTCCCT GAGGGGGAAG CCTTTCCCCC TGTCTCCGTC
ACCACTCTGG GCTCTCCCAT GCATTCAAAC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21050
Accession Version NM_001285987.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 798bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 10-AUG-2015
Organism Homo sapiens (human)
Product POU domain, class 5, transcription factor 1 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ486514.1, DQ486515.1, Z11899.1 and AI811039.1. Summary: This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]. Transcript Variant: This variant (5, also known as OCT4B) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate AUG start codon, compared to variant 1. The resulting isoform (3, also known as OCT4B-265) is shorter and has a distinct N-terminus, compared to isoform 1. This variant represents an allele of variant 2 that contains an AUG start codon that is polymorphic in human populations (see rs3130932). This variant may encode additional isoforms through the use of alternative downstream AUG and non-AUG start codons, as described in PMID:19489092. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ486515.1, BC020712.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCACTTCT ACAGACTATT CCTTGGGGCC ACACGTAGGT TCTTGAATCC CGAATGGAAA 
GGGGAGATTG ATAACTGGTG TGTTTATGTT CTTACAAGTC TTCTGCCTTT TAAAATCCAG
TCCCAGGACA TCAAAGCTCT GCAGAAAGAA CTCGAGCAAT TTGCCAAGCT CCTGAAGCAG
AAGAGGATCA CCCTGGGATA TACACAGGCC GATGTGGGGC TCACCCTGGG GGTTCTATTT
GGGAAGGTAT TCAGCCAAAC GACCATCTGC CGCTTTGAGG CTCTGCAGCT TAGCTTCAAG
AACATGTGTA AGCTGCGGCC CTTGCTGCAG AAGTGGGTGG AGGAAGCTGA CAACAATGAA
AATCTTCAGG AGATATGCAA AGCAGAAACC CTCGTGCAGG CCCGAAAGAG AAAGCGAACC
AGTATCGAGA ACCGAGTGAG AGGCAACCTG GAGAATTTGT TCCTGCAGTG CCCGAAACCC
ACACTGCAGC AGATCAGCCA CATCGCCCAG CAGCTTGGGC TCGAGAAGGA TGTGGTCCGA
GTGTGGTTCT GTAACCGGCG CCAGAAGGGC AAGCGATCAA GCAGCGACTA TGCACAACGA
GAGGATTTTG AGGCTGCTGG GTCTCCTTTC TCAGGGGGAC CAGTGTCCTT TCCTCTGGCC
CCAGGGCCCC ATTTTGGTAC CCCAGGCTAT GGGAGCCCTC ACTTCACTGC ACTGTACTCC
TCGGTCCCTT TCCCTGAGGG GGAAGCCTTT CCCCCTGTCT CCGTCACCAC TCTGGGCTCT
CCCATGCATT CAAACTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001272916.1
CDS1004..1801
Misc Feature(1)974..976(+)
Misc Feature(2)1130..1354(+)
Misc Feature(3)1229..1231(+)
Misc Feature(4)1307..1309(+)
Misc Feature(5)1409..1585(+)
Misc Feature(6)1409..1579(+)
Misc Feature(7)1415..1567(+)
Exon (1)1..1244
Gene:POU5F1
Gene Synonym:
Exon (2)1245..1375
Gene:POU5F1
Gene Synonym:
Exon (3)1376..1534
Gene:POU5F1
Gene Synonym:
Exon (4)1535..2065
Gene:POU5F1
Gene Synonym:
Translation
Position Chain Variation Link
1 1 a, g dbSNP:756575303
15 15 c, t dbSNP:556183524
38 38 a, g dbSNP:767061672
60 60 a, g dbSNP:3130931
91 91 a, g dbSNP:750002991
106 106 a, t dbSNP:573550776
115 115 a, g dbSNP:76465289
135 135 c, g dbSNP:536581051
151 151 g, t dbSNP:769146675
162 162 a, g dbSNP:566021008
179 179 a, g dbSNP:749630858
206 206 a, g dbSNP:776087600
221 221 a, g dbSNP:200340326
223 223 -, gag dbSNP:9281235
223 223 g, t dbSNP:77085553
224 224 -, gag dbSNP:72545985
225 225 -, gag, tga dbSNP:67207605
226 226 -, aag, gag dbSNP:141270342
227 227 g, t dbSNP:201576321
229 229 a, c dbSNP:553951373
283 283 -, gt dbSNP:571828146
323 323 c, t dbSNP:72856737
331 331 a, c dbSNP:571668450
333 333 c, g dbSNP:759635638
348 348 a, t dbSNP:545437201
349 349 c, t dbSNP:9263800
357 357 a, g dbSNP:11965454
407 407 c, g dbSNP:567697919
416 416 c, t dbSNP:3757349
450 450 a, t dbSNP:775390135
466 466 g, t dbSNP:73401031
496 496 -, cttt dbSNP:550996676
497 497 -, t dbSNP:533341709
507 507 -, tt dbSNP:67692102
507 507 -, t dbSNP:764189579
508 508 c, t dbSNP:201876558
508 508 -, t dbSNP:9279005
508 508 -, t dbSNP:757541562
509 509 c, t dbSNP:200941459
520 520 -, ag dbSNP:776463063
546 546 a, g dbSNP:574609806
587 587 a, c dbSNP:141017974
625 625 a, g dbSNP:151095308
659 659 c, t dbSNP:533115866
683 683 c, t dbSNP:779561772
744 744 c, t dbSNP:562465318
759 759 c, t dbSNP:770180344
773 773 a, g dbSNP:3132526
779 779 c, t dbSNP:573636729
784 784 c, t dbSNP:756964786
824 824 c, g dbSNP:746682206
825 825 -, gc dbSNP:28728473
825 825 -, gc dbSNP:745327767
825 825 c, t dbSNP:777344152
826 826 -, c, ctc dbSNP:9281234
826 826 c, g, t dbSNP:71563310
827 827 -, c, ctc dbSNP:72545983
828 828 -, ctc dbSNP:67257409
828 828 -, tct dbSNP:766283576
829 829 -, ctc dbSNP:79966288
830 830 a, g dbSNP:752409659
839 839 g, t dbSNP:561385932
844 844 c, t dbSNP:765257293
860 860 c, t dbSNP:754865454
861 861 c, g dbSNP:1265163
908 908 c, t dbSNP:572416729
909 909 c, t dbSNP:761916552
915 915 a, t dbSNP:115513668
941 941 a, t dbSNP:58535985
942 942 g, t dbSNP:58132172
966 966 a, g dbSNP:76364340
983 983 a, g dbSNP:763461206
1001 1001 c, t dbSNP:775689149
1005 1005 g, t dbSNP:3130932
1007 1007 a, c dbSNP:746349953
1014 1014 a, g dbSNP:374060997
1021 1021 a, g dbSNP:770437114
1026 1026 c, t dbSNP:751818073
1028 1028 a, g dbSNP:777447714
1038 1038 a, g, t dbSNP:748039347
1054 1054 c, g, t dbSNP:9501063
1058 1058 c, t dbSNP:753723388
1062 1062 a, g dbSNP:780099361
1064 1064 c, g dbSNP:374746302
1068 1068 a, t dbSNP:751667986
1069 1069 a, g dbSNP:764458539
1078 1078 a, c, t dbSNP:72856736
1088 1088 g, t dbSNP:765521397
1091 1091 a, g dbSNP:759883550
1094 1094 a, c dbSNP:777021724
1096 1096 a, c, t dbSNP:760230615
1099 1099 a, g dbSNP:772698923
1103 1103 a, c dbSNP:771780451
1106 1106 c, t dbSNP:747761780
1121 1121 c, t dbSNP:778571821
1125 1125 a, c dbSNP:768359010
1135 1135 c, t dbSNP:749071230
1140 1140 c, t dbSNP:149025884
1143 1143 c, t dbSNP:137936040
1156 1156 c, t dbSNP:751745427
1157 1157 a, g dbSNP:777993058
1162 1162 a, g dbSNP:150320288
1222 1222 a, g dbSNP:752871883
1246 1246 a, g dbSNP:754269967
1264 1264 a, g dbSNP:373808092
1302 1302 a, g dbSNP:756413624
1318 1318 g, t dbSNP:115234511
1319 1319 c, t dbSNP:768029325
1364 1364 a, t dbSNP:572480837
1373 1373 a, g dbSNP:773889469
1375 1375 a, g dbSNP:763710527
1394 1394 c, t dbSNP:1150767
1396 1396 c, t dbSNP:780201728
1397 1397 a, c, g dbSNP:750755680
1402 1402 a, g dbSNP:767958442
1429 1429 c, t dbSNP:757769384
1437 1437 a, g dbSNP:751096375
1438 1438 a, t dbSNP:368434272
1447 1447 c, t dbSNP:578180451
1462 1462 g, t dbSNP:775301501
1477 1477 a, g dbSNP:764939136
1483 1483 c, g dbSNP:17851818
1489 1489 a, g dbSNP:776478147
1500 1500 a, g dbSNP:770844532
1507 1507 c, t dbSNP:746974556
1525 1525 c, t dbSNP:772966815
1526 1526 a, g dbSNP:768746587
1529 1529 a, g dbSNP:749326531
1536 1536 g, t dbSNP:770067555
1542 1542 a, g dbSNP:746104715
1552 1552 c, t dbSNP:781212227
1586 1586 a, g dbSNP:771345851
1588 1588 c, t dbSNP:141766253
1589 1589 a, g dbSNP:778460021
1596 1596 c, t dbSNP:546320542
1599 1599 -, aac dbSNP:765569430
1613 1613 a, g dbSNP:758948344
1626 1626 c, t dbSNP:752325450
1637 1637 a, g dbSNP:778574549
1669 1669 a, g dbSNP:754481951
1684 1684 c, g dbSNP:753553081
1691 1691 g, t dbSNP:765910375
1719 1719 a, c dbSNP:760558360
1725 1725 c, t dbSNP:750163518
1726 1726 a, g dbSNP:767500519
1738 1738 g, t dbSNP:761734933
1756 1756 a, c dbSNP:774206292
1757 1757 c, t dbSNP:769875118
1765 1765 c, t dbSNP:1061118
1770 1770 c, t dbSNP:1061120
1773 1773 c, g dbSNP:776940833
1780 1780 c, t dbSNP:771024256
1788 1788 a, t dbSNP:747446765
1812 1812 c, t dbSNP:564148752
1830 1830 -, a dbSNP:759833918
1830 1830 a, g dbSNP:772503540
1832 1832 a, g dbSNP:748680648
1842 1842 a, g dbSNP:779517517
1845 1845 a, g dbSNP:754549702
1857 1857 a, g dbSNP:1061126
1876 1876 a, g dbSNP:3734864
1927 1927 -, aa dbSNP:60004964
1927 1927 a, c dbSNP:200275741
1948 1948 -, t dbSNP:746543478
2003 2003 c, t dbSNP:537327593
2037 2037 -, gcc dbSNP:750617181
2039 2039 c, t dbSNP:13409

Target ORF information:

RefSeq Version NM_001285987
Organism Homo sapiens (human)
Definition Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001285987

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCACTTCT ACAGACTATT CCTTGGGGCC ACACGTAGGT TCTTGAATCC CGAATGGAAA 
GGGGAGATTG ATAACTGGTG TGTTTATGTT CTTACAAGTC TTCTGCCTTT TAAAATCCAG
TCCCAGGACA TCAAAGCTCT GCAGAAAGAA CTCGAGCAAT TTGCCAAGCT CCTGAAGCAG
AAGAGGATCA CCCTGGGATA TACACAGGCC GATGTGGGGC TCACCCTGGG GGTTCTATTT
GGGAAGGTAT TCAGCCAAAC GACCATCTGC CGCTTTGAGG CTCTGCAGCT TAGCTTCAAG
AACATGTGTA AGCTGCGGCC CTTGCTGCAG AAGTGGGTGG AGGAAGCTGA CAACAATGAA
AATCTTCAGG AGATATGCAA AGCAGAAACC CTCGTGCAGG CCCGAAAGAG AAAGCGAACC
AGTATCGAGA ACCGAGTGAG AGGCAACCTG GAGAATTTGT TCCTGCAGTG CCCGAAACCC
ACACTGCAGC AGATCAGCCA CATCGCCCAG CAGCTTGGGC TCGAGAAGGA TGTGGTCCGA
GTGTGGTTCT GTAACCGGCG CCAGAAGGGC AAGCGATCAA GCAGCGACTA TGCACAACGA
GAGGATTTTG AGGCTGCTGG GTCTCCTTTC TCAGGGGGAC CAGTGTCCTT TCCTCTGGCC
CCAGGGCCCC ATTTTGGTAC CCCAGGCTAT GGGAGCCCTC ACTTCACTGC ACTGTACTCC
TCGGTCCCTT TCCCTGAGGG GGAAGCCTTT CCCCCTGTCT CCGTCACCAC TCTGGGCTCT
CCCATGCATT CAAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20361
Accession Version NM_002701.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1083bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu20361D_COA.pdf (pdf)
Update Date 10-AUG-2015
Organism Homo sapiens (human)
Product POU domain, class 5, transcription factor 1 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD247724.1, BC117435.1, DQ486517.1 and AI811039.1. On Oct 26, 2013 this sequence version replaced gi:116235483. Summary: This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]. Transcript Variant: This variant (1, also known as OCT4A) represents the shortest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z11898.1, BC069246.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCGGGAC ACCTGGCTTC GGATTTCGCC TTCTCGCCCC CTCCAGGTGG TGGAGGTGAT 
GGGCCAGGGG GGCCGGAGCC GGGCTGGGTT GATCCTCGGA CCTGGCTAAG CTTCCAAGGC
CCTCCTGGAG GGCCAGGAAT CGGGCCGGGG GTTGGGCCAG GCTCTGAGGT GTGGGGGATT
CCCCCATGCC CCCCGCCGTA TGAGTTCTGT GGGGGGATGG CGTACTGTGG GCCCCAGGTT
GGAGTGGGGC TAGTGCCCCA AGGCGGCTTG GAGACCTCTC AGCCTGAGGG CGAAGCAGGA
GTCGGGGTGG AGAGCAACTC CGATGGGGCC TCCCCGGAGC CCTGCACCGT CACCCCTGGT
GCCGTGAAGC TGGAGAAGGA GAAGCTGGAG CAAAACCCGG AGGAGTCCCA GGACATCAAA
GCTCTGCAGA AAGAACTCGA GCAATTTGCC AAGCTCCTGA AGCAGAAGAG GATCACCCTG
GGATATACAC AGGCCGATGT GGGGCTCACC CTGGGGGTTC TATTTGGGAA GGTATTCAGC
CAAACGACCA TCTGCCGCTT TGAGGCTCTG CAGCTTAGCT TCAAGAACAT GTGTAAGCTG
CGGCCCTTGC TGCAGAAGTG GGTGGAGGAA GCTGACAACA ATGAAAATCT TCAGGAGATA
TGCAAAGCAG AAACCCTCGT GCAGGCCCGA AAGAGAAAGC GAACCAGTAT CGAGAACCGA
GTGAGAGGCA ACCTGGAGAA TTTGTTCCTG CAGTGCCCGA AACCCACACT GCAGCAGATC
AGCCACATCG CCCAGCAGCT TGGGCTCGAG AAGGATGTGG TCCGAGTGTG GTTCTGTAAC
CGGCGCCAGA AGGGCAAGCG ATCAAGCAGC GACTATGCAC AACGAGAGGA TTTTGAGGCT
GCTGGGTCTC CTTTCTCAGG GGGACCAGTG TCCTTTCCTC TGGCCCCAGG GCCCCATTTT
GGTACCCCAG GCTATGGGAG CCCTCACTTC ACTGCACTGT ACTCCTCGGT CCCTTTCCCT
GAGGGGGAAG CCTTTCCCCC TGTCTCCGTC ACCACTCTGG GCTCTCCCAT GCATTCAAAC
TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_002692.2
CDS74..1156
Misc Feature(1)11..13(+)
Misc Feature(2)404..406(+)
Misc Feature(3)485..709(+)
Misc Feature(4)764..940(+)
Misc Feature(5)764..934(+)
Misc Feature(6)770..922(+)
Misc Feature(7)776..778(+)
Misc Feature(8)779..781(+)
Misc Feature(9)938..940(+)
Misc Feature(10)941..943(+)
Misc Feature(11)1136..1138(+)
Exon (1)1..478
Gene:POU5F1
Gene Synonym:
Exon (2)479..599
Gene:POU5F1
Gene Synonym:
Exon (3)600..730
Gene:POU5F1
Gene Synonym:
Exon (4)731..889
Gene:POU5F1
Gene Synonym:
Exon (5)890..1420
Gene:POU5F1
Gene Synonym:
Translation
Position Chain Variation Link
24 24 c, t dbSNP:546382046
28 28 a, c dbSNP:528219311
29 29 g, t dbSNP:762460472
34 34 c, g dbSNP:775039003
35 35 a, g dbSNP:746492273
43 43 a, g dbSNP:745711427
47 47 c, g dbSNP:781107051
50 50 c, t dbSNP:563793031
51 51 a, g dbSNP:545405466
52 52 a, g dbSNP:774824120
60 60 c, t dbSNP:192146552
61 61 a, g, t dbSNP:753905289
67 67 c, t dbSNP:780186724
72 72 c, t dbSNP:563060750
78 78 c, t dbSNP:750853618
79 79 a, g, t dbSNP:762107693
84 84 a, c dbSNP:752015029
86 86 c, t dbSNP:764622871
94 94 a, g dbSNP:2077010
100 100 c, t dbSNP:1265160
104 104 g, t dbSNP:769613294
109 109 a, g dbSNP:759247300
110 110 c, t dbSNP:555966532
114 114 c, t dbSNP:770851738
126 126 a, c, g dbSNP:773044360
136 136 a, g dbSNP:117585298
140 140 c, g dbSNP:749367658
148 148 a, g dbSNP:780177765
149 149 a, g dbSNP:267600952
150 150 -, agccgg dbSNP:747042206
153 153 c, t dbSNP:555977384
154 154 a, g dbSNP:756386879
160 160 g, t dbSNP:200769740
161 161 a, g, t dbSNP:41257954
168 168 c, t dbSNP:751905954
170 170 c, t dbSNP:558239925
171 171 a, g dbSNP:758911777
184 184 c, t dbSNP:752219633
192 192 a, g dbSNP:764849092
196 196 a, t dbSNP:759046399
203 203 a, g dbSNP:776451971
214 214 c, t dbSNP:766077489
215 215 a, g dbSNP:760434609
219 219 c, t dbSNP:773026654
220 220 a, g dbSNP:539854667
222 222 a, g dbSNP:771970606
223 223 a, g dbSNP:569265492
231 231 c, t dbSNP:748102586
238 238 c, t dbSNP:774216881
250 250 a, g dbSNP:550687611
261 261 a, g dbSNP:745910390
267 267 -, c dbSNP:758644856
267 267 c, t dbSNP:77172244
268 268 a, c, g dbSNP:747375993
270 270 c, t dbSNP:374685641
271 271 a, g dbSNP:758629425
284 284 g, t dbSNP:753248478
286 286 a, g dbSNP:546394200
287 287 c, g dbSNP:754555422
295 295 a, g dbSNP:528231599
300 300 g, t dbSNP:766174312
301 301 c, t dbSNP:760370244
310 310 c, g dbSNP:750243037
312 312 g, t dbSNP:767358536
314 314 a, g dbSNP:370690490
335 335 a, g dbSNP:774304660
337 337 a, c, t dbSNP:201790772
338 338 a, g dbSNP:776664777
341 341 c, t dbSNP:771128794
350 350 c, t dbSNP:747246352
364 364 c, t dbSNP:1062630
370 370 a, g, t dbSNP:141749005
372 372 g, t dbSNP:138735965
373 373 a, t dbSNP:755508064
376 376 c, t dbSNP:753463537
377 377 a, g dbSNP:779696279
380 380 a, g dbSNP:755521189
385 385 c, g dbSNP:750095390
390 390 a, g dbSNP:767038169
394 394 c, t dbSNP:187901814
395 395 g, t dbSNP:751334783
399 399 a, g dbSNP:764061072
408 408 c, t dbSNP:762875743
409 409 a, g dbSNP:776752950
416 416 c, t dbSNP:771043396
421 421 c, t dbSNP:530499672
422 422 a, g dbSNP:182726472
426 426 c, t dbSNP:144901157
427 427 c, g dbSNP:772160653
432 432 a, g dbSNP:748561847
436 436 c, t dbSNP:779414190
438 438 a, t dbSNP:769344602
441 441 a, g dbSNP:749826964
446 446 a, g dbSNP:780359280
447 447 a, g dbSNP:755681827
448 448 g, t dbSNP:749854246
449 449 -, aag dbSNP:748004013
457 457 a, g dbSNP:541450349
471 471 c, t dbSNP:574320983
472 472 a, g dbSNP:562319329
480 480 a, c dbSNP:768359010
490 490 c, t dbSNP:749071230
495 495 c, t dbSNP:149025884
498 498 c, t dbSNP:137936040
511 511 c, t dbSNP:751745427
512 512 a, g dbSNP:777993058
517 517 a, g dbSNP:150320288
577 577 a, g dbSNP:752871883
601 601 a, g dbSNP:754269967
619 619 a, g dbSNP:373808092
657 657 a, g dbSNP:756413624
673 673 g, t dbSNP:115234511
674 674 c, t dbSNP:768029325
719 719 a, t dbSNP:572480837
728 728 a, g dbSNP:773889469
730 730 a, g dbSNP:763710527
749 749 c, t dbSNP:1150767
751 751 c, t dbSNP:780201728
752 752 a, c, g dbSNP:750755680
757 757 a, g dbSNP:767958442
784 784 c, t dbSNP:757769384
792 792 a, g dbSNP:751096375
793 793 a, t dbSNP:368434272
802 802 c, t dbSNP:578180451
817 817 g, t dbSNP:775301501
832 832 a, g dbSNP:764939136
838 838 c, g dbSNP:17851818
844 844 a, g dbSNP:776478147
855 855 a, g dbSNP:770844532
862 862 c, t dbSNP:746974556
880 880 c, t dbSNP:772966815
881 881 a, g dbSNP:768746587
884 884 a, g dbSNP:749326531
891 891 g, t dbSNP:770067555
897 897 a, g dbSNP:746104715
907 907 c, t dbSNP:781212227
941 941 a, g dbSNP:771345851
943 943 c, t dbSNP:141766253
944 944 a, g dbSNP:778460021
951 951 c, t dbSNP:546320542
954 954 -, aac dbSNP:765569430
968 968 a, g dbSNP:758948344
981 981 c, t dbSNP:752325450
992 992 a, g dbSNP:778574549
1024 1024 a, g dbSNP:754481951
1039 1039 c, g dbSNP:753553081
1046 1046 g, t dbSNP:765910375
1074 1074 a, c dbSNP:760558360
1080 1080 c, t dbSNP:750163518
1081 1081 a, g dbSNP:767500519
1093 1093 g, t dbSNP:761734933
1111 1111 a, c dbSNP:774206292
1112 1112 c, t dbSNP:769875118
1120 1120 c, t dbSNP:1061118
1125 1125 c, t dbSNP:1061120
1128 1128 c, g dbSNP:776940833
1135 1135 c, t dbSNP:771024256
1143 1143 a, t dbSNP:747446765
1167 1167 c, t dbSNP:564148752
1185 1185 -, a dbSNP:759833918
1185 1185 a, g dbSNP:772503540
1187 1187 a, g dbSNP:748680648
1197 1197 a, g dbSNP:779517517
1200 1200 a, g dbSNP:754549702
1212 1212 a, g dbSNP:1061126
1231 1231 a, g dbSNP:3734864
1282 1282 -, aa dbSNP:60004964
1282 1282 a, c dbSNP:200275741
1303 1303 -, t dbSNP:746543478
1358 1358 c, t dbSNP:537327593
1392 1392 -, gcc dbSNP:750617181
1394 1394 c, t dbSNP:13409

Target ORF information:

RefSeq Version NM_002701
Organism Homo sapiens (human)
Definition Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002701

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCGGGAC ACCTGGCTTC GGATTTCGCC TTCTCGCCCC CTCCAGGTGG TGGAGGTGAT 
GGGCCAGGGG GGCCGGAGCC GGGCTGGGTT GATCCTCGGA CCTGGCTAAG CTTCCAAGGC
CCTCCTGGAG GGCCAGGAAT CGGGCCGGGG GTTGGGCCAG GCTCTGAGGT GTGGGGGATT
CCCCCATGCC CCCCGCCGTA TGAGTTCTGT GGGGGGATGG CGTACTGTGG GCCCCAGGTT
GGAGTGGGGC TAGTGCCCCA AGGCGGCTTG GAGACCTCTC AGCCTGAGGG CGAAGCAGGA
GTCGGGGTGG AGAGCAACTC CGATGGGGCC TCCCCGGAGC CCTGCACCGT CACCCCTGGT
GCCGTGAAGC TGGAGAAGGA GAAGCTGGAG CAAAACCCGG AGGAGTCCCA GGACATCAAA
GCTCTGCAGA AAGAACTCGA GCAATTTGCC AAGCTCCTGA AGCAGAAGAG GATCACCCTG
GGATATACAC AGGCCGATGT GGGGCTCACC CTGGGGGTTC TATTTGGGAA GGTATTCAGC
CAAACGACCA TCTGCCGCTT TGAGGCTCTG CAGCTTAGCT TCAAGAACAT GTGTAAGCTG
CGGCCCTTGC TGCAGAAGTG GGTGGAGGAA GCTGACAACA ATGAAAATCT TCAGGAGATA
TGCAAAGCAG AAACCCTCGT GCAGGCCCGA AAGAGAAAGC GAACCAGTAT CGAGAACCGA
GTGAGAGGCA ACCTGGAGAA TTTGTTCCTG CAGTGCCCGA AACCCACACT GCAGCAGATC
AGCCACATCG CCCAGCAGCT TGGGCTCGAG AAGGATGTGG TCCGAGTGTG GTTCTGTAAC
CGGCGCCAGA AGGGCAAGCG ATCAAGCAGC GACTATGCAC AACGAGAGGA TTTTGAGGCT
GCTGGGTCTC CTTTCTCAGG GGGACCAGTG TCCTTTCCTC TGGCCCCAGG GCCCCATTTT
GGTACCCCAG GCTATGGGAG CCCTCACTTC ACTGCACTGT ACTCCTCGGT CCCTTTCCCT
GAGGGGGAAG CCTTTCCCCC TGTCTCCGTC ACCACTCTGG GCTCTCCCAT GCATTCAAAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21117
Accession Version NM_001285986.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 495bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 10-AUG-2015
Organism Homo sapiens (human)
Product POU domain, class 5, transcription factor 1 isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ486514.1, DQ486515.1 and AI811039.1. Summary: This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]. Transcript Variant: This variant (4, also known as OCT4B1) contains multiple differences in the 5' UTR and the 5' coding region, compared to variant 1, and initiates translation at a downstream in-frame AUG start codon. The resulting isoform (4, also known as OCT4B-164) is shorter at the N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: DQ486515.1, BC020712.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGTGTAAGC TGCGGCCCTT GCTGCAGAAG TGGGTGGAGG AAGCTGACAA CAATGAAAAT 
CTTCAGGAGA TATGCAAAGC AGAAACCCTC GTGCAGGCCC GAAAGAGAAA GCGAACCAGT
ATCGAGAACC GAGTGAGAGG CAACCTGGAG AATTTGTTCC TGCAGTGCCC GAAACCCACA
CTGCAGCAGA TCAGCCACAT CGCCCAGCAG CTTGGGCTCG AGAAGGATGT GGTCCGAGTG
TGGTTCTGTA ACCGGCGCCA GAAGGGCAAG CGATCAAGCA GCGACTATGC ACAACGAGAG
GATTTTGAGG CTGCTGGGTC TCCTTTCTCA GGGGGACCAG TGTCCTTTCC TCTGGCCCCA
GGGCCCCATT TTGGTACCCC AGGCTATGGG AGCCCTCACT TCACTGCACT GTACTCCTCG
GTCCCTTTCC CTGAGGGGGA AGCCTTTCCC CCTGTCTCCG TCACCACTCT GGGCTCTCCC
ATGCATTCAA ACTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001272915.1
CDS1532..2026
Misc Feature(1)1349..1351(+)
Misc Feature(2)1532..1579(+)
Misc Feature(3)1634..1810(+)
Misc Feature(4)1634..1804(+)
Misc Feature(5)1640..1792(+)
Exon (1)1..1600
Gene:POU5F1
Gene Synonym:
Exon (2)1601..1759
Gene:POU5F1
Gene Synonym:
Exon (3)1760..2290
Gene:POU5F1
Gene Synonym:
Translation
Position Chain Variation Link
1 1 a, g dbSNP:756575303
15 15 c, t dbSNP:556183524
38 38 a, g dbSNP:767061672
60 60 a, g dbSNP:3130931
91 91 a, g dbSNP:750002991
106 106 a, t dbSNP:573550776
115 115 a, g dbSNP:76465289
135 135 c, g dbSNP:536581051
151 151 g, t dbSNP:769146675
162 162 a, g dbSNP:566021008
179 179 a, g dbSNP:749630858
206 206 a, g dbSNP:776087600
221 221 a, g dbSNP:200340326
223 223 -, gag dbSNP:9281235
223 223 g, t dbSNP:77085553
224 224 -, gag dbSNP:72545985
225 225 -, gag, tga dbSNP:67207605
226 226 -, aag, gag dbSNP:141270342
227 227 g, t dbSNP:201576321
229 229 a, c dbSNP:553951373
283 283 -, gt dbSNP:571828146
323 323 c, t dbSNP:72856737
331 331 a, c dbSNP:571668450
333 333 c, g dbSNP:759635638
348 348 a, t dbSNP:545437201
349 349 c, t dbSNP:9263800
357 357 a, g dbSNP:11965454
407 407 c, g dbSNP:567697919
416 416 c, t dbSNP:3757349
450 450 a, t dbSNP:775390135
466 466 g, t dbSNP:73401031
496 496 -, cttt dbSNP:550996676
497 497 -, t dbSNP:533341709
507 507 -, tt dbSNP:67692102
507 507 -, t dbSNP:764189579
508 508 c, t dbSNP:201876558
508 508 -, t dbSNP:9279005
508 508 -, t dbSNP:757541562
509 509 c, t dbSNP:200941459
520 520 -, ag dbSNP:776463063
546 546 a, g dbSNP:574609806
587 587 a, c dbSNP:141017974
625 625 a, g dbSNP:151095308
659 659 c, t dbSNP:533115866
683 683 c, t dbSNP:779561772
744 744 c, t dbSNP:562465318
759 759 c, t dbSNP:770180344
773 773 a, g dbSNP:3132526
779 779 c, t dbSNP:573636729
784 784 c, t dbSNP:756964786
824 824 c, g dbSNP:746682206
825 825 -, gc dbSNP:28728473
825 825 -, gc dbSNP:745327767
825 825 c, t dbSNP:777344152
826 826 -, c, ctc dbSNP:9281234
826 826 c, g, t dbSNP:71563310
827 827 -, c, ctc dbSNP:72545983
828 828 -, ctc dbSNP:67257409
828 828 -, tct dbSNP:766283576
829 829 -, ctc dbSNP:79966288
830 830 a, g dbSNP:752409659
839 839 g, t dbSNP:561385932
844 844 c, t dbSNP:765257293
860 860 c, t dbSNP:754865454
861 861 c, g dbSNP:1265163
908 908 c, t dbSNP:572416729
909 909 c, t dbSNP:761916552
915 915 a, t dbSNP:115513668
941 941 a, t dbSNP:58535985
942 942 g, t dbSNP:58132172
966 966 a, g dbSNP:76364340
983 983 a, g dbSNP:763461206
1001 1001 c, t dbSNP:775689149
1005 1005 g, t dbSNP:3130932
1007 1007 a, c dbSNP:746349953
1014 1014 a, g dbSNP:374060997
1021 1021 a, g dbSNP:770437114
1026 1026 c, t dbSNP:751818073
1028 1028 a, g dbSNP:777447714
1038 1038 a, g, t dbSNP:748039347
1054 1054 c, g, t dbSNP:9501063
1058 1058 c, t dbSNP:753723388
1062 1062 a, g dbSNP:780099361
1064 1064 c, g dbSNP:374746302
1068 1068 a, t dbSNP:751667986
1069 1069 a, g dbSNP:764458539
1078 1078 a, c, t dbSNP:72856736
1088 1088 g, t dbSNP:765521397
1091 1091 a, g dbSNP:759883550
1094 1094 a, c dbSNP:777021724
1096 1096 a, c, t dbSNP:760230615
1099 1099 a, g dbSNP:772698923
1103 1103 a, c dbSNP:771780451
1106 1106 c, t dbSNP:747761780
1121 1121 c, t dbSNP:778571821
1125 1125 a, c dbSNP:768359010
1135 1135 c, t dbSNP:749071230
1140 1140 c, t dbSNP:149025884
1143 1143 c, t dbSNP:137936040
1156 1156 c, t dbSNP:751745427
1157 1157 a, g dbSNP:777993058
1162 1162 a, g dbSNP:150320288
1222 1222 a, g dbSNP:752871883
1253 1253 c, t dbSNP:765446871
1255 1255 c, t dbSNP:368865757
1260 1260 a, c dbSNP:372561846
1265 1265 -, t dbSNP:200370996
1271 1271 c, t dbSNP:45506394
1272 1272 a, g, t dbSNP:773644692
1279 1279 c, t dbSNP:771496772
1281 1281 -, c dbSNP:767101644
1281 1281 c, t dbSNP:761338834
1285 1285 a, g dbSNP:45438402
1305 1305 a, g dbSNP:534141870
1314 1314 g, t dbSNP:566787524
1317 1317 a, c dbSNP:761782197
1323 1323 a, c dbSNP:776548442
1325 1325 -, ct dbSNP:9281233
1326 1326 -, ct, tc dbSNP:3064871
1327 1327 -, ct, cta dbSNP:34631505
1327 1327 c, t dbSNP:764010389
1366 1366 -, gcttgg dbSNP:74525213
1367 1367 -, gg dbSNP:200714691
1368 1368 -, ttgggc dbSNP:111545081
1369 1369 -, gggctt dbSNP:143097483
1370 1370 -, gggctt dbSNP:145919884
1371 1371 -, gcttgg dbSNP:149056926
1371 1371 -, ggcttt dbSNP:367730301
1371 1371 g, t dbSNP:755546995
1384 1384 a, g dbSNP:551624054
1387 1387 a, c dbSNP:533052915
1420 1420 a, g dbSNP:768323673
1427 1427 c, t dbSNP:187046732
1428 1428 a, g dbSNP:756724092
1434 1434 c, t dbSNP:769916722
1435 1435 c, g dbSNP:745803283
1439 1439 c, t dbSNP:2106074
1449 1449 -, ctttt dbSNP:761437827
1449 1449 c, t dbSNP:200784165
1457 1457 c, t dbSNP:748214914
1460 1460 c, t dbSNP:779057746
1464 1464 c, t dbSNP:367652843
1471 1471 a, g dbSNP:754269967
1489 1489 a, g dbSNP:373808092
1527 1527 a, g dbSNP:756413624
1543 1543 g, t dbSNP:115234511
1544 1544 c, t dbSNP:768029325
1589 1589 a, t dbSNP:572480837
1598 1598 a, g dbSNP:773889469
1600 1600 a, g dbSNP:763710527
1619 1619 c, t dbSNP:1150767
1621 1621 c, t dbSNP:780201728
1622 1622 a, c, g dbSNP:750755680
1627 1627 a, g dbSNP:767958442
1654 1654 c, t dbSNP:757769384
1662 1662 a, g dbSNP:751096375
1663 1663 a, t dbSNP:368434272
1672 1672 c, t dbSNP:578180451
1687 1687 g, t dbSNP:775301501
1702 1702 a, g dbSNP:764939136
1708 1708 c, g dbSNP:17851818
1714 1714 a, g dbSNP:776478147
1725 1725 a, g dbSNP:770844532
1732 1732 c, t dbSNP:746974556
1750 1750 c, t dbSNP:772966815
1751 1751 a, g dbSNP:768746587
1754 1754 a, g dbSNP:749326531
1761 1761 g, t dbSNP:770067555
1767 1767 a, g dbSNP:746104715
1777 1777 c, t dbSNP:781212227
1811 1811 a, g dbSNP:771345851
1813 1813 c, t dbSNP:141766253
1814 1814 a, g dbSNP:778460021
1821 1821 c, t dbSNP:546320542
1824 1824 -, aac dbSNP:765569430
1838 1838 a, g dbSNP:758948344
1851 1851 c, t dbSNP:752325450
1862 1862 a, g dbSNP:778574549
1894 1894 a, g dbSNP:754481951
1909 1909 c, g dbSNP:753553081
1916 1916 g, t dbSNP:765910375
1944 1944 a, c dbSNP:760558360
1950 1950 c, t dbSNP:750163518
1951 1951 a, g dbSNP:767500519
1963 1963 g, t dbSNP:761734933
1981 1981 a, c dbSNP:774206292
1982 1982 c, t dbSNP:769875118
1990 1990 c, t dbSNP:1061118
1995 1995 c, t dbSNP:1061120
1998 1998 c, g dbSNP:776940833
2005 2005 c, t dbSNP:771024256
2013 2013 a, t dbSNP:747446765
2037 2037 c, t dbSNP:564148752
2055 2055 -, a dbSNP:759833918
2055 2055 a, g dbSNP:772503540
2057 2057 a, g dbSNP:748680648
2067 2067 a, g dbSNP:779517517
2070 2070 a, g dbSNP:754549702
2082 2082 a, g dbSNP:1061126
2101 2101 a, g dbSNP:3734864
2152 2152 -, aa dbSNP:60004964
2152 2152 a, c dbSNP:200275741
2173 2173 -, t dbSNP:746543478
2228 2228 c, t dbSNP:537327593
2262 2262 -, gcc dbSNP:750617181
2264 2264 c, t dbSNP:13409

Target ORF information:

RefSeq Version NM_001285986
Organism Homo sapiens (human)
Definition Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001285986

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGTGTAAGC TGCGGCCCTT GCTGCAGAAG TGGGTGGAGG AAGCTGACAA CAATGAAAAT 
CTTCAGGAGA TATGCAAAGC AGAAACCCTC GTGCAGGCCC GAAAGAGAAA GCGAACCAGT
ATCGAGAACC GAGTGAGAGG CAACCTGGAG AATTTGTTCC TGCAGTGCCC GAAACCCACA
CTGCAGCAGA TCAGCCACAT CGCCCAGCAG CTTGGGCTCG AGAAGGATGT GGTCCGAGTG
TGGTTCTGTA ACCGGCGCCA GAAGGGCAAG CGATCAAGCA GCGACTATGC ACAACGAGAG
GATTTTGAGG CTGCTGGGTC TCCTTTCTCA GGGGGACCAG TGTCCTTTCC TCTGGCCCCA
GGGCCCCATT TTGGTACCCC AGGCTATGGG AGCCCTCACT TCACTGCACT GTACTCCTCG
GTCCCTTTCC CTGAGGGGGA AGCCTTTCCC CCTGTCTCCG TCACCACTCT GGGCTCTCCC
ATGCATTCAA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20662
Accession Version NM_203289.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 573bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 10-AUG-2015
Organism Homo sapiens (human)
Product POU domain, class 5, transcription factor 1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ486514.1, DQ486515.1 and AI811039.1. On Oct 26, 2013 this sequence version replaced gi:291167754. Summary: This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]. Transcript Variant: This variant (2, also known as OCT4B) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame non-AUG (CUG) start codon, compared to variant 1. The resulting isoform (2, also known as OCT4B-190) is shorter at the N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2). This variant may encode additional isoforms through the use of an alternative downstream AUG start codon, as well as an alternative upstream AUG start codon, which is polymorphic in human populations (AGG allele represented in this RefSeq; see rs3130932). Use of alternate start codons and the non-AUG start codon is described in PMID:19489092. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ486515.1, BC020712.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 19489092 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
CTGGGGGTTC TATTTGGGAA GGTATTCAGC CAAACGACCA TCTGCCGCTT TGAGGCTCTG 
CAGCTTAGCT TCAAGAACAT GTGTAAGCTG CGGCCCTTGC TGCAGAAGTG GGTGGAGGAA
GCTGACAACA ATGAAAATCT TCAGGAGATA TGCAAAGCAG AAACCCTCGT GCAGGCCCGA
AAGAGAAAGC GAACCAGTAT CGAGAACCGA GTGAGAGGCA ACCTGGAGAA TTTGTTCCTG
CAGTGCCCGA AACCCACACT GCAGCAGATC AGCCACATCG CCCAGCAGCT TGGGCTCGAG
AAGGATGTGG TCCGAGTGTG GTTCTGTAAC CGGCGCCAGA AGGGCAAGCG ATCAAGCAGC
GACTATGCAC AACGAGAGGA TTTTGAGGCT GCTGGGTCTC CTTTCTCAGG GGGACCAGTG
TCCTTTCCTC TGGCCCCAGG GCCCCATTTT GGTACCCCAG GCTATGGGAG CCCTCACTTC
ACTGCACTGT ACTCCTCGGT CCCTTTCCCT GAGGGGGAAG CCTTTCCCCC TGTCTCCGTC
ACCACTCTGG GCTCTCCCAT GCATTCAAAC TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_976034.4
CDS1229..1801
Misc Feature(1)974..976(+)
Misc Feature(2)1004..1006(+)
Misc Feature(3)1229..1354(+)
Misc Feature(4)1307..1309(+)
Misc Feature(5)1409..1585(+)
Misc Feature(6)1409..1579(+)
Misc Feature(7)1415..1567(+)
Exon (1)1..1244
Gene:POU5F1
Gene Synonym:
Exon (2)1245..1375
Gene:POU5F1
Gene Synonym:
Exon (3)1376..1534
Gene:POU5F1
Gene Synonym:
Exon (4)1535..2065
Gene:POU5F1
Gene Synonym:
Translation
Position Chain Variation Link
1 1 a, g dbSNP:756575303
15 15 c, t dbSNP:556183524
38 38 a, g dbSNP:767061672
60 60 a, g dbSNP:3130931
91 91 a, g dbSNP:750002991
106 106 a, t dbSNP:573550776
115 115 a, g dbSNP:76465289
135 135 c, g dbSNP:536581051
151 151 g, t dbSNP:769146675
162 162 a, g dbSNP:566021008
179 179 a, g dbSNP:749630858
206 206 a, g dbSNP:776087600
221 221 a, g dbSNP:200340326
223 223 -, gag dbSNP:9281235
223 223 g, t dbSNP:77085553
224 224 -, gag dbSNP:72545985
225 225 -, gag, tga dbSNP:67207605
226 226 -, aag, gag dbSNP:141270342
227 227 g, t dbSNP:201576321
229 229 a, c dbSNP:553951373
283 283 -, gt dbSNP:571828146
323 323 c, t dbSNP:72856737
331 331 a, c dbSNP:571668450
333 333 c, g dbSNP:759635638
348 348 a, t dbSNP:545437201
349 349 c, t dbSNP:9263800
357 357 a, g dbSNP:11965454
407 407 c, g dbSNP:567697919
416 416 c, t dbSNP:3757349
450 450 a, t dbSNP:775390135
466 466 g, t dbSNP:73401031
496 496 -, cttt dbSNP:550996676
497 497 -, t dbSNP:533341709
507 507 -, tt dbSNP:67692102
507 507 -, t dbSNP:764189579
508 508 c, t dbSNP:201876558
508 508 -, t dbSNP:9279005
508 508 -, t dbSNP:757541562
509 509 c, t dbSNP:200941459
520 520 -, ag dbSNP:776463063
546 546 a, g dbSNP:574609806
587 587 a, c dbSNP:141017974
625 625 a, g dbSNP:151095308
659 659 c, t dbSNP:533115866
683 683 c, t dbSNP:779561772
744 744 c, t dbSNP:562465318
759 759 c, t dbSNP:770180344
773 773 a, g dbSNP:3132526
779 779 c, t dbSNP:573636729
784 784 c, t dbSNP:756964786
824 824 c, g dbSNP:746682206
825 825 -, gc dbSNP:28728473
825 825 -, gc dbSNP:745327767
825 825 c, t dbSNP:777344152
826 826 -, c, ctc dbSNP:9281234
826 826 c, g, t dbSNP:71563310
827 827 -, c, ctc dbSNP:72545983
828 828 -, ctc dbSNP:67257409
828 828 -, tct dbSNP:766283576
829 829 -, ctc dbSNP:79966288
830 830 a, g dbSNP:752409659
839 839 g, t dbSNP:561385932
844 844 c, t dbSNP:765257293
860 860 c, t dbSNP:754865454
861 861 c, g dbSNP:1265163
908 908 c, t dbSNP:572416729
909 909 c, t dbSNP:761916552
915 915 a, t dbSNP:115513668
941 941 a, t dbSNP:58535985
942 942 g, t dbSNP:58132172
966 966 a, g dbSNP:76364340
983 983 a, g dbSNP:763461206
1001 1001 c, t dbSNP:775689149
1005 1005 g, t dbSNP:3130932
1007 1007 a, c dbSNP:746349953
1014 1014 a, g dbSNP:374060997
1021 1021 a, g dbSNP:770437114
1026 1026 c, t dbSNP:751818073
1028 1028 a, g dbSNP:777447714
1038 1038 a, g, t dbSNP:748039347
1054 1054 c, g, t dbSNP:9501063
1058 1058 c, t dbSNP:753723388
1062 1062 a, g dbSNP:780099361
1064 1064 c, g dbSNP:374746302
1068 1068 a, t dbSNP:751667986
1069 1069 a, g dbSNP:764458539
1078 1078 a, c, t dbSNP:72856736
1088 1088 g, t dbSNP:765521397
1091 1091 a, g dbSNP:759883550
1094 1094 a, c dbSNP:777021724
1096 1096 a, c, t dbSNP:760230615
1099 1099 a, g dbSNP:772698923
1103 1103 a, c dbSNP:771780451
1106 1106 c, t dbSNP:747761780
1121 1121 c, t dbSNP:778571821
1125 1125 a, c dbSNP:768359010
1135 1135 c, t dbSNP:749071230
1140 1140 c, t dbSNP:149025884
1143 1143 c, t dbSNP:137936040
1156 1156 c, t dbSNP:751745427
1157 1157 a, g dbSNP:777993058
1162 1162 a, g dbSNP:150320288
1222 1222 a, g dbSNP:752871883
1246 1246 a, g dbSNP:754269967
1264 1264 a, g dbSNP:373808092
1302 1302 a, g dbSNP:756413624
1318 1318 g, t dbSNP:115234511
1319 1319 c, t dbSNP:768029325
1364 1364 a, t dbSNP:572480837
1373 1373 a, g dbSNP:773889469
1375 1375 a, g dbSNP:763710527
1394 1394 c, t dbSNP:1150767
1396 1396 c, t dbSNP:780201728
1397 1397 a, c, g dbSNP:750755680
1402 1402 a, g dbSNP:767958442
1429 1429 c, t dbSNP:757769384
1437 1437 a, g dbSNP:751096375
1438 1438 a, t dbSNP:368434272
1447 1447 c, t dbSNP:578180451
1462 1462 g, t dbSNP:775301501
1477 1477 a, g dbSNP:764939136
1483 1483 c, g dbSNP:17851818
1489 1489 a, g dbSNP:776478147
1500 1500 a, g dbSNP:770844532
1507 1507 c, t dbSNP:746974556
1525 1525 c, t dbSNP:772966815
1526 1526 a, g dbSNP:768746587
1529 1529 a, g dbSNP:749326531
1536 1536 g, t dbSNP:770067555
1542 1542 a, g dbSNP:746104715
1552 1552 c, t dbSNP:781212227
1586 1586 a, g dbSNP:771345851
1588 1588 c, t dbSNP:141766253
1589 1589 a, g dbSNP:778460021
1596 1596 c, t dbSNP:546320542
1599 1599 -, aac dbSNP:765569430
1613 1613 a, g dbSNP:758948344
1626 1626 c, t dbSNP:752325450
1637 1637 a, g dbSNP:778574549
1669 1669 a, g dbSNP:754481951
1684 1684 c, g dbSNP:753553081
1691 1691 g, t dbSNP:765910375
1719 1719 a, c dbSNP:760558360
1725 1725 c, t dbSNP:750163518
1726 1726 a, g dbSNP:767500519
1738 1738 g, t dbSNP:761734933
1756 1756 a, c dbSNP:774206292
1757 1757 c, t dbSNP:769875118
1765 1765 c, t dbSNP:1061118
1770 1770 c, t dbSNP:1061120
1773 1773 c, g dbSNP:776940833
1780 1780 c, t dbSNP:771024256
1788 1788 a, t dbSNP:747446765
1812 1812 c, t dbSNP:564148752
1830 1830 -, a dbSNP:759833918
1830 1830 a, g dbSNP:772503540
1832 1832 a, g dbSNP:748680648
1842 1842 a, g dbSNP:779517517
1845 1845 a, g dbSNP:754549702
1857 1857 a, g dbSNP:1061126
1876 1876 a, g dbSNP:3734864
1927 1927 -, aa dbSNP:60004964
1927 1927 a, c dbSNP:200275741
1948 1948 -, t dbSNP:746543478
2003 2003 c, t dbSNP:537327593
2037 2037 -, gcc dbSNP:750617181
2039 2039 c, t dbSNP:13409

Target ORF information:

RefSeq Version NM_203289
Organism Homo sapiens (human)
Definition Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_203289

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
CTGGGGGTTC TATTTGGGAA GGTATTCAGC CAAACGACCA TCTGCCGCTT TGAGGCTCTG 
CAGCTTAGCT TCAAGAACAT GTGTAAGCTG CGGCCCTTGC TGCAGAAGTG GGTGGAGGAA
GCTGACAACA ATGAAAATCT TCAGGAGATA TGCAAAGCAG AAACCCTCGT GCAGGCCCGA
AAGAGAAAGC GAACCAGTAT CGAGAACCGA GTGAGAGGCA ACCTGGAGAA TTTGTTCCTG
CAGTGCCCGA AACCCACACT GCAGCAGATC AGCCACATCG CCCAGCAGCT TGGGCTCGAG
AAGGATGTGG TCCGAGTGTG GTTCTGTAAC CGGCGCCAGA AGGGCAAGCG ATCAAGCAGC
GACTATGCAC AACGAGAGGA TTTTGAGGCT GCTGGGTCTC CTTTCTCAGG GGGACCAGTG
TCCTTTCCTC TGGCCCCAGG GCCCCATTTT GGTACCCCAG GCTATGGGAG CCCTCACTTC
ACTGCACTGT ACTCCTCGGT CCCTTTCCCT GAGGGGGAAG CCTTTCCCCC TGTCTCCGTC
ACCACTCTGG GCTCTCCCAT GCATTCAAAC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.