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POU5F1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol POU5F1
Entrez Gene ID 5460
Full Name POU class 5 homeobox 1
Synonyms OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4
General protein information
Preferred Names
POU domain, class 5, transcription factor 1
Names
POU domain, class 5, transcription factor 1
octamer-binding protein 3
octamer-binding protein 4
POU domain transcription factor OCT4
octamer-binding transcription factor 3
octamer-binding transcription factor-3
POU-type homeodomain-containing DNA-binding protein
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p21.31

Summary This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]. lac of sum
Disorder MIM:

164177


mRNA and Protein(s)

mRNA Protein Name
NM_001173531 NP_001167002 POU domain, class 5, transcription factor 1 isoform 2
NM_001285987 NP_001272916 POU domain, class 5, transcription factor 1 isoform 3
NM_002701 NP_002692 POU domain, class 5, transcription factor 1 isoform 1
NM_001285986 NP_001272915 POU domain, class 5, transcription factor 1 isoform 4
NM_203289 NP_976034 POU domain, class 5, transcription factor 1 isoform 2



Homo sapiens (human) POU5F1 NP_002692.2
Pan troglodytes (chimpanzee) POU5F1 NP_001238970.1
Macaca mulatta (Rhesus monkey) POU5F1 NP_001108427.1
Canis lupus familiaris (dog) POU5F1 XP_538830.1
Bos taurus (cattle) POU5F1 NP_777005.1
Mus musculus (house mouse) Pou5f1 NP_038661.2
Rattus norvegicus (Norway rat) Pou5f1 NP_001009178.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POU5F1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POU5F1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu20662 NM_001173531 Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
OHu21050 NM_001285987 Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu20361
NM_002701 Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu21117 NM_001285986 Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu20662 NM_203289 Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20662
Clone ID Related Accession (Same CDS sequence) NM_001173531 , NM_203289
Accession Version NM_001173531.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 573bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 10-AUG-2015
Organism Homo sapiens (human)
Product POU domain, class 5, transcription factor 1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ486514.1, DQ486516.1, DQ486515.1 and AI811039.1. On Oct 26, 2013 this sequence version replaced gi:291167756. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame non-AUG (CUG) start codon, compared to variant 1. The resulting isoform (2, also known as OCT4B-190) is shorter at the N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2). This variant may encode an additional isoform through the use of an alternative downstream AUG start codon. Use of alternate start codons and the non-AUG start codon is described in PMID:19489092. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ486516.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2150385, SAMEA2157511 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 19489092 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
CTGGGGGTTC TATTTGGGAA GGTATTCAGC CAAACGACCA TCTGCCGCTT TGAGGCTCTG 
CAGCTTAGCT TCAAGAACAT GTGTAAGCTG CGGCCCTTGC TGCAGAAGTG GGTGGAGGAA
GCTGACAACA ATGAAAATCT TCAGGAGATA TGCAAAGCAG AAACCCTCGT GCAGGCCCGA
AAGAGAAAGC GAACCAGTAT CGAGAACCGA GTGAGAGGCA ACCTGGAGAA TTTGTTCCTG
CAGTGCCCGA AACCCACACT GCAGCAGATC AGCCACATCG CCCAGCAGCT TGGGCTCGAG
AAGGATGTGG TCCGAGTGTG GTTCTGTAAC CGGCGCCAGA AGGGCAAGCG ATCAAGCAGC
GACTATGCAC AACGAGAGGA TTTTGAGGCT GCTGGGTCTC CTTTCTCAGG GGGACCAGTG
TCCTTTCCTC TGGCCCCAGG GCCCCATTTT GGTACCCCAG GCTATGGGAG CCCTCACTTC
ACTGCACTGT ACTCCTCGGT CCCTTTCCCT GAGGGGGAAG CCTTTCCCCC TGTCTCCGTC
ACCACTCTGG GCTCTCCCAT GCATTCAAAC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001167002.1
CDS743..1315
Misc Feature(1)623..625(+)
Misc Feature(2)743..868(+)
Misc Feature(3)821..823(+)
Misc Feature(4)923..1099(+)
Misc Feature(5)923..1093(+)
Misc Feature(6)929..1081(+)
Exon (1)1..637
Gene:POU5F1
Gene Synonym:
Exon (2)638..758
Gene:POU5F1
Gene Synonym:
Exon (3)759..889
Gene:POU5F1
Gene Synonym:
Exon (4)890..1048
Gene:POU5F1
Gene Synonym:
Exon (5)1049..1579
Gene:POU5F1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001173531
Organism Homo sapiens (human)
Definition Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001173531

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
CTGGGGGTTC TATTTGGGAA GGTATTCAGC CAAACGACCA TCTGCCGCTT TGAGGCTCTG 
CAGCTTAGCT TCAAGAACAT GTGTAAGCTG CGGCCCTTGC TGCAGAAGTG GGTGGAGGAA
GCTGACAACA ATGAAAATCT TCAGGAGATA TGCAAAGCAG AAACCCTCGT GCAGGCCCGA
AAGAGAAAGC GAACCAGTAT CGAGAACCGA GTGAGAGGCA ACCTGGAGAA TTTGTTCCTG
CAGTGCCCGA AACCCACACT GCAGCAGATC AGCCACATCG CCCAGCAGCT TGGGCTCGAG
AAGGATGTGG TCCGAGTGTG GTTCTGTAAC CGGCGCCAGA AGGGCAAGCG ATCAAGCAGC
GACTATGCAC AACGAGAGGA TTTTGAGGCT GCTGGGTCTC CTTTCTCAGG GGGACCAGTG
TCCTTTCCTC TGGCCCCAGG GCCCCATTTT GGTACCCCAG GCTATGGGAG CCCTCACTTC
ACTGCACTGT ACTCCTCGGT CCCTTTCCCT GAGGGGGAAG CCTTTCCCCC TGTCTCCGTC
ACCACTCTGG GCTCTCCCAT GCATTCAAAC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21050
Clone ID Related Accession (Same CDS sequence) NM_001285987
Accession Version NM_001285987.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 798bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 10-AUG-2015
Organism Homo sapiens (human)
Product POU domain, class 5, transcription factor 1 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ486514.1, DQ486515.1, Z11899.1 and AI811039.1. Transcript Variant: This variant (5, also known as OCT4B) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate AUG start codon, compared to variant 1. The resulting isoform (3, also known as OCT4B-265) is shorter and has a distinct N-terminus, compared to isoform 1. This variant represents an allele of variant 2 that contains an AUG start codon that is polymorphic in human populations (see rs3130932). This variant may encode additional isoforms through the use of alternative downstream AUG and non-AUG start codons, as described in PMID:19489092. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ486515.1, BC020712.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCACTTCT ACAGACTATT CCTTGGGGCC ACACGTAGGT TCTTGAATCC CGAATGGAAA 
GGGGAGATTG ATAACTGGTG TGTTTATGTT CTTACAAGTC TTCTGCCTTT TAAAATCCAG
TCCCAGGACA TCAAAGCTCT GCAGAAAGAA CTCGAGCAAT TTGCCAAGCT CCTGAAGCAG
AAGAGGATCA CCCTGGGATA TACACAGGCC GATGTGGGGC TCACCCTGGG GGTTCTATTT
GGGAAGGTAT TCAGCCAAAC GACCATCTGC CGCTTTGAGG CTCTGCAGCT TAGCTTCAAG
AACATGTGTA AGCTGCGGCC CTTGCTGCAG AAGTGGGTGG AGGAAGCTGA CAACAATGAA
AATCTTCAGG AGATATGCAA AGCAGAAACC CTCGTGCAGG CCCGAAAGAG AAAGCGAACC
AGTATCGAGA ACCGAGTGAG AGGCAACCTG GAGAATTTGT TCCTGCAGTG CCCGAAACCC
ACACTGCAGC AGATCAGCCA CATCGCCCAG CAGCTTGGGC TCGAGAAGGA TGTGGTCCGA
GTGTGGTTCT GTAACCGGCG CCAGAAGGGC AAGCGATCAA GCAGCGACTA TGCACAACGA
GAGGATTTTG AGGCTGCTGG GTCTCCTTTC TCAGGGGGAC CAGTGTCCTT TCCTCTGGCC
CCAGGGCCCC ATTTTGGTAC CCCAGGCTAT GGGAGCCCTC ACTTCACTGC ACTGTACTCC
TCGGTCCCTT TCCCTGAGGG GGAAGCCTTT CCCCCTGTCT CCGTCACCAC TCTGGGCTCT
CCCATGCATT CAAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001272916.1
CDS1004..1801
Misc Feature(1)974..976(+)
Misc Feature(2)1130..1354(+)
Misc Feature(3)1229..1231(+)
Misc Feature(4)1307..1309(+)
Misc Feature(5)1409..1585(+)
Misc Feature(6)1409..1579(+)
Misc Feature(7)1415..1567(+)
Exon (1)1..1244
Gene:POU5F1
Gene Synonym:
Exon (2)1245..1375
Gene:POU5F1
Gene Synonym:
Exon (3)1376..1534
Gene:POU5F1
Gene Synonym:
Exon (4)1535..2065
Gene:POU5F1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001285987
Organism Homo sapiens (human)
Definition Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001285987

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCACTTCT ACAGACTATT CCTTGGGGCC ACACGTAGGT TCTTGAATCC CGAATGGAAA 
GGGGAGATTG ATAACTGGTG TGTTTATGTT CTTACAAGTC TTCTGCCTTT TAAAATCCAG
TCCCAGGACA TCAAAGCTCT GCAGAAAGAA CTCGAGCAAT TTGCCAAGCT CCTGAAGCAG
AAGAGGATCA CCCTGGGATA TACACAGGCC GATGTGGGGC TCACCCTGGG GGTTCTATTT
GGGAAGGTAT TCAGCCAAAC GACCATCTGC CGCTTTGAGG CTCTGCAGCT TAGCTTCAAG
AACATGTGTA AGCTGCGGCC CTTGCTGCAG AAGTGGGTGG AGGAAGCTGA CAACAATGAA
AATCTTCAGG AGATATGCAA AGCAGAAACC CTCGTGCAGG CCCGAAAGAG AAAGCGAACC
AGTATCGAGA ACCGAGTGAG AGGCAACCTG GAGAATTTGT TCCTGCAGTG CCCGAAACCC
ACACTGCAGC AGATCAGCCA CATCGCCCAG CAGCTTGGGC TCGAGAAGGA TGTGGTCCGA
GTGTGGTTCT GTAACCGGCG CCAGAAGGGC AAGCGATCAA GCAGCGACTA TGCACAACGA
GAGGATTTTG AGGCTGCTGG GTCTCCTTTC TCAGGGGGAC CAGTGTCCTT TCCTCTGGCC
CCAGGGCCCC ATTTTGGTAC CCCAGGCTAT GGGAGCCCTC ACTTCACTGC ACTGTACTCC
TCGGTCCCTT TCCCTGAGGG GGAAGCCTTT CCCCCTGTCT CCGTCACCAC TCTGGGCTCT
CCCATGCATT CAAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20361
Clone ID Related Accession (Same CDS sequence) NM_002701
Accession Version NM_002701.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1083bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 10-AUG-2015
Organism Homo sapiens (human)
Product POU domain, class 5, transcription factor 1 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD247724.1, BC117435.1, DQ486517.1 and AI811039.1. On Oct 26, 2013 this sequence version replaced gi:116235483. Transcript Variant: This variant (1, also known as OCT4A) represents the shortest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z11898.1, BC069246.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCGGGAC ACCTGGCTTC GGATTTCGCC TTCTCGCCCC CTCCAGGTGG TGGAGGTGAT 
GGGCCAGGGG GGCCGGAGCC GGGCTGGGTT GATCCTCGGA CCTGGCTAAG CTTCCAAGGC
CCTCCTGGAG GGCCAGGAAT CGGGCCGGGG GTTGGGCCAG GCTCTGAGGT GTGGGGGATT
CCCCCATGCC CCCCGCCGTA TGAGTTCTGT GGGGGGATGG CGTACTGTGG GCCCCAGGTT
GGAGTGGGGC TAGTGCCCCA AGGCGGCTTG GAGACCTCTC AGCCTGAGGG CGAAGCAGGA
GTCGGGGTGG AGAGCAACTC CGATGGGGCC TCCCCGGAGC CCTGCACCGT CACCCCTGGT
GCCGTGAAGC TGGAGAAGGA GAAGCTGGAG CAAAACCCGG AGGAGTCCCA GGACATCAAA
GCTCTGCAGA AAGAACTCGA GCAATTTGCC AAGCTCCTGA AGCAGAAGAG GATCACCCTG
GGATATACAC AGGCCGATGT GGGGCTCACC CTGGGGGTTC TATTTGGGAA GGTATTCAGC
CAAACGACCA TCTGCCGCTT TGAGGCTCTG CAGCTTAGCT TCAAGAACAT GTGTAAGCTG
CGGCCCTTGC TGCAGAAGTG GGTGGAGGAA GCTGACAACA ATGAAAATCT TCAGGAGATA
TGCAAAGCAG AAACCCTCGT GCAGGCCCGA AAGAGAAAGC GAACCAGTAT CGAGAACCGA
GTGAGAGGCA ACCTGGAGAA TTTGTTCCTG CAGTGCCCGA AACCCACACT GCAGCAGATC
AGCCACATCG CCCAGCAGCT TGGGCTCGAG AAGGATGTGG TCCGAGTGTG GTTCTGTAAC
CGGCGCCAGA AGGGCAAGCG ATCAAGCAGC GACTATGCAC AACGAGAGGA TTTTGAGGCT
GCTGGGTCTC CTTTCTCAGG GGGACCAGTG TCCTTTCCTC TGGCCCCAGG GCCCCATTTT
GGTACCCCAG GCTATGGGAG CCCTCACTTC ACTGCACTGT ACTCCTCGGT CCCTTTCCCT
GAGGGGGAAG CCTTTCCCCC TGTCTCCGTC ACCACTCTGG GCTCTCCCAT GCATTCAAAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002692.2
CDS74..1156
Misc Feature(1)11..13(+)
Misc Feature(2)404..406(+)
Misc Feature(3)485..709(+)
Misc Feature(4)764..940(+)
Misc Feature(5)764..934(+)
Misc Feature(6)770..922(+)
Misc Feature(7)776..778(+)
Misc Feature(8)779..781(+)
Misc Feature(9)938..940(+)
Misc Feature(10)941..943(+)
Misc Feature(11)1136..1138(+)
Exon (1)1..478
Gene:POU5F1
Gene Synonym:
Exon (2)479..599
Gene:POU5F1
Gene Synonym:
Exon (3)600..730
Gene:POU5F1
Gene Synonym:
Exon (4)731..889
Gene:POU5F1
Gene Synonym:
Exon (5)890..1420
Gene:POU5F1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002701
Organism Homo sapiens (human)
Definition Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002701

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCGGGAC ACCTGGCTTC GGATTTCGCC TTCTCGCCCC CTCCAGGTGG TGGAGGTGAT 
GGGCCAGGGG GGCCGGAGCC GGGCTGGGTT GATCCTCGGA CCTGGCTAAG CTTCCAAGGC
CCTCCTGGAG GGCCAGGAAT CGGGCCGGGG GTTGGGCCAG GCTCTGAGGT GTGGGGGATT
CCCCCATGCC CCCCGCCGTA TGAGTTCTGT GGGGGGATGG CGTACTGTGG GCCCCAGGTT
GGAGTGGGGC TAGTGCCCCA AGGCGGCTTG GAGACCTCTC AGCCTGAGGG CGAAGCAGGA
GTCGGGGTGG AGAGCAACTC CGATGGGGCC TCCCCGGAGC CCTGCACCGT CACCCCTGGT
GCCGTGAAGC TGGAGAAGGA GAAGCTGGAG CAAAACCCGG AGGAGTCCCA GGACATCAAA
GCTCTGCAGA AAGAACTCGA GCAATTTGCC AAGCTCCTGA AGCAGAAGAG GATCACCCTG
GGATATACAC AGGCCGATGT GGGGCTCACC CTGGGGGTTC TATTTGGGAA GGTATTCAGC
CAAACGACCA TCTGCCGCTT TGAGGCTCTG CAGCTTAGCT TCAAGAACAT GTGTAAGCTG
CGGCCCTTGC TGCAGAAGTG GGTGGAGGAA GCTGACAACA ATGAAAATCT TCAGGAGATA
TGCAAAGCAG AAACCCTCGT GCAGGCCCGA AAGAGAAAGC GAACCAGTAT CGAGAACCGA
GTGAGAGGCA ACCTGGAGAA TTTGTTCCTG CAGTGCCCGA AACCCACACT GCAGCAGATC
AGCCACATCG CCCAGCAGCT TGGGCTCGAG AAGGATGTGG TCCGAGTGTG GTTCTGTAAC
CGGCGCCAGA AGGGCAAGCG ATCAAGCAGC GACTATGCAC AACGAGAGGA TTTTGAGGCT
GCTGGGTCTC CTTTCTCAGG GGGACCAGTG TCCTTTCCTC TGGCCCCAGG GCCCCATTTT
GGTACCCCAG GCTATGGGAG CCCTCACTTC ACTGCACTGT ACTCCTCGGT CCCTTTCCCT
GAGGGGGAAG CCTTTCCCCC TGTCTCCGTC ACCACTCTGG GCTCTCCCAT GCATTCAAAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21117
Clone ID Related Accession (Same CDS sequence) NM_001285986
Accession Version NM_001285986.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 495bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 10-AUG-2015
Organism Homo sapiens (human)
Product POU domain, class 5, transcription factor 1 isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ486514.1, DQ486515.1 and AI811039.1. Transcript Variant: This variant (4, also known as OCT4B1) contains multiple differences in the 5' UTR and the 5' coding region, compared to variant 1, and initiates translation at a downstream in-frame AUG start codon. The resulting isoform (4, also known as OCT4B-164) is shorter at the N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: DQ486515.1, BC020712.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGTGTAAGC TGCGGCCCTT GCTGCAGAAG TGGGTGGAGG AAGCTGACAA CAATGAAAAT 
CTTCAGGAGA TATGCAAAGC AGAAACCCTC GTGCAGGCCC GAAAGAGAAA GCGAACCAGT
ATCGAGAACC GAGTGAGAGG CAACCTGGAG AATTTGTTCC TGCAGTGCCC GAAACCCACA
CTGCAGCAGA TCAGCCACAT CGCCCAGCAG CTTGGGCTCG AGAAGGATGT GGTCCGAGTG
TGGTTCTGTA ACCGGCGCCA GAAGGGCAAG CGATCAAGCA GCGACTATGC ACAACGAGAG
GATTTTGAGG CTGCTGGGTC TCCTTTCTCA GGGGGACCAG TGTCCTTTCC TCTGGCCCCA
GGGCCCCATT TTGGTACCCC AGGCTATGGG AGCCCTCACT TCACTGCACT GTACTCCTCG
GTCCCTTTCC CTGAGGGGGA AGCCTTTCCC CCTGTCTCCG TCACCACTCT GGGCTCTCCC
ATGCATTCAA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001272915.1
CDS1532..2026
Misc Feature(1)1349..1351(+)
Misc Feature(2)1532..1579(+)
Misc Feature(3)1634..1810(+)
Misc Feature(4)1634..1804(+)
Misc Feature(5)1640..1792(+)
Exon (1)1..1600
Gene:POU5F1
Gene Synonym:
Exon (2)1601..1759
Gene:POU5F1
Gene Synonym:
Exon (3)1760..2290
Gene:POU5F1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001285986
Organism Homo sapiens (human)
Definition Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001285986

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGTGTAAGC TGCGGCCCTT GCTGCAGAAG TGGGTGGAGG AAGCTGACAA CAATGAAAAT 
CTTCAGGAGA TATGCAAAGC AGAAACCCTC GTGCAGGCCC GAAAGAGAAA GCGAACCAGT
ATCGAGAACC GAGTGAGAGG CAACCTGGAG AATTTGTTCC TGCAGTGCCC GAAACCCACA
CTGCAGCAGA TCAGCCACAT CGCCCAGCAG CTTGGGCTCG AGAAGGATGT GGTCCGAGTG
TGGTTCTGTA ACCGGCGCCA GAAGGGCAAG CGATCAAGCA GCGACTATGC ACAACGAGAG
GATTTTGAGG CTGCTGGGTC TCCTTTCTCA GGGGGACCAG TGTCCTTTCC TCTGGCCCCA
GGGCCCCATT TTGGTACCCC AGGCTATGGG AGCCCTCACT TCACTGCACT GTACTCCTCG
GTCCCTTTCC CTGAGGGGGA AGCCTTTCCC CCTGTCTCCG TCACCACTCT GGGCTCTCCC
ATGCATTCAA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20662
Clone ID Related Accession (Same CDS sequence) NM_001173531 , NM_203289
Accession Version NM_203289.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 573bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 10-AUG-2015
Organism Homo sapiens (human)
Product POU domain, class 5, transcription factor 1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ486514.1, DQ486515.1 and AI811039.1. On Oct 26, 2013 this sequence version replaced gi:291167754. Transcript Variant: This variant (2, also known as OCT4B) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame non-AUG (CUG) start codon, compared to variant 1. The resulting isoform (2, also known as OCT4B-190) is shorter at the N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2). This variant may encode additional isoforms through the use of an alternative downstream AUG start codon, as well as an alternative upstream AUG start codon, which is polymorphic in human populations (AGG allele represented in this RefSeq; see rs3130932). Use of alternate start codons and the non-AUG start codon is described in PMID:19489092. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ486515.1, BC020712.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 19489092 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
CTGGGGGTTC TATTTGGGAA GGTATTCAGC CAAACGACCA TCTGCCGCTT TGAGGCTCTG 
CAGCTTAGCT TCAAGAACAT GTGTAAGCTG CGGCCCTTGC TGCAGAAGTG GGTGGAGGAA
GCTGACAACA ATGAAAATCT TCAGGAGATA TGCAAAGCAG AAACCCTCGT GCAGGCCCGA
AAGAGAAAGC GAACCAGTAT CGAGAACCGA GTGAGAGGCA ACCTGGAGAA TTTGTTCCTG
CAGTGCCCGA AACCCACACT GCAGCAGATC AGCCACATCG CCCAGCAGCT TGGGCTCGAG
AAGGATGTGG TCCGAGTGTG GTTCTGTAAC CGGCGCCAGA AGGGCAAGCG ATCAAGCAGC
GACTATGCAC AACGAGAGGA TTTTGAGGCT GCTGGGTCTC CTTTCTCAGG GGGACCAGTG
TCCTTTCCTC TGGCCCCAGG GCCCCATTTT GGTACCCCAG GCTATGGGAG CCCTCACTTC
ACTGCACTGT ACTCCTCGGT CCCTTTCCCT GAGGGGGAAG CCTTTCCCCC TGTCTCCGTC
ACCACTCTGG GCTCTCCCAT GCATTCAAAC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_976034.4
CDS1229..1801
Misc Feature(1)974..976(+)
Misc Feature(2)1004..1006(+)
Misc Feature(3)1229..1354(+)
Misc Feature(4)1307..1309(+)
Misc Feature(5)1409..1585(+)
Misc Feature(6)1409..1579(+)
Misc Feature(7)1415..1567(+)
Exon (1)1..1244
Gene:POU5F1
Gene Synonym:
Exon (2)1245..1375
Gene:POU5F1
Gene Synonym:
Exon (3)1376..1534
Gene:POU5F1
Gene Synonym:
Exon (4)1535..2065
Gene:POU5F1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_203289
Organism Homo sapiens (human)
Definition Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_203289

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
CTGGGGGTTC TATTTGGGAA GGTATTCAGC CAAACGACCA TCTGCCGCTT TGAGGCTCTG 
CAGCTTAGCT TCAAGAACAT GTGTAAGCTG CGGCCCTTGC TGCAGAAGTG GGTGGAGGAA
GCTGACAACA ATGAAAATCT TCAGGAGATA TGCAAAGCAG AAACCCTCGT GCAGGCCCGA
AAGAGAAAGC GAACCAGTAT CGAGAACCGA GTGAGAGGCA ACCTGGAGAA TTTGTTCCTG
CAGTGCCCGA AACCCACACT GCAGCAGATC AGCCACATCG CCCAGCAGCT TGGGCTCGAG
AAGGATGTGG TCCGAGTGTG GTTCTGTAAC CGGCGCCAGA AGGGCAAGCG ATCAAGCAGC
GACTATGCAC AACGAGAGGA TTTTGAGGCT GCTGGGTCTC CTTTCTCAGG GGGACCAGTG
TCCTTTCCTC TGGCCCCAGG GCCCCATTTT GGTACCCCAG GCTATGGGAG CCCTCACTTC
ACTGCACTGT ACTCCTCGGT CCCTTTCCCT GAGGGGGAAG CCTTTCCCCC TGTCTCCGTC
ACCACTCTGG GCTCTCCCAT GCATTCAAAC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Prolonged proteasome inhibition cyclically upregulates Oct3/4 and Nanog gene expression, but reduces induced pluripotent stem cell colony formation
Cell Reprogram 17 (2), 95-105 (2015)
Floyd EZ, Staszkiewicz J, Power RA, Kilroy G, Kirk-Ballard H, Barnes CW, Strickler KL, Rim JS, Harkins LL, Gao R, Kim J and Eilertsen KJ.


book

Genetic determinants associated with early age of diagnosis of IBD
Dis. Colon Rectum 58 (3), 321-327 (2015)
Connelly TM, Berg AS, Harris L 3rd, Brinton D, Deiling S and Koltun WA.


book

Concise review: isoforms of OCT4 contribute to the confusing diversity in stem cell biology
Stem Cells 28 (5), 885-893 (2010)
Wang X and Dai J.


book

Mapping of the minimal internal ribosome entry site element in the human embryonic stem cell gene OCT4B mRNA
Biochem. Biophys. Res. Commun. 394 (3), 750-754 (2010)
Zhang W, Wang X, Xiao Z, Liu W, Chen B and Dai J.


book

Alternative translation of OCT4 by an internal ribosome entry site and its novel function in stress response
Stem Cells 27 (6), 1265-1275 (2009)
Wang X, Zhao Y, Xiao Z, Chen B, Wei Z, Wang B, Zhang J, Han J, Gao Y, Li L, Zhao H, Zhao W, Lin H and Dai J.


book

OCT4 spliced variants are differentially expressed in human pluripotent and nonpluripotent cells
Stem Cells 26 (12), 3068-3074 (2008)
Atlasi Y, Mowla SJ, Ziaee SA, Gokhale PJ and Andrews PW.


book

The human OCT-4 isoforms differ in their ability to confer self-renewal
J. Biol. Chem. 281 (44), 33554-33565 (2006)
Lee J, Kim HK, Rho JY, Han YM and Kim J.


book

A human POU domain gene, mPOU, is expressed in developing brain and specific adult tissues
Eur. J. Biochem. 220 (3), 753-762 (1994)
Wey E, Lyons GE and Schafer BW.


book

Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissues
Nucleic Acids Res. 20 (17), 4613-4620 (1992)
Takeda J, Seino S and Bell GI.


book

Octamer-dependent regulation of the kFGF gene in embryonal carcinoma and embryonic stem cells
Mech. Dev. 36 (1-2), 75-86 (1991)
Schoorlemmer J and Kruijer W.


 
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