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PPAT cDNA ORF clone, Homo sapiens (human)

Gene Symbol PPAT
Entrez Gene ID 5471
Full Name phosphoribosyl pyrophosphate amidotransferase
Synonyms ATASE, GPAT, PRAT
General protein information
Preferred Names
amidophosphoribosyltransferase
Names
amidophosphoribosyltransferase
glutamine PRPP amidotransferase
glutamine phosphoribosylpyrophosphate amidotransferase
glutamine phosphoribosylpyrophosphatate amidotransferase
NP_002694.3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

4

4q12

Summary The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq, Mar 2011]. lac of sum
Disorder MIM:

172450

Disorder Html:





The following PPAT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPAT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu59843 XM_011534387 PREDICTED: Homo sapiens phosphoribosyl pyrophosphate amidotransferase (PPAT), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu03961 NM_002703 Homo sapiens phosphoribosyl pyrophosphate amidotransferase (PPAT), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$307.30
$439.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu59843
Accession Version XM_011534387.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1311bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product amidophosphoribosyltransferase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022853.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGGAGTTC GGTGCCAACA TTCAAATCAC ACAAGGTATG CCACCACAGG AAAATGTGAA 
CTAGAAAATT GTCAGCCCTT CGTTGTTGAA ACACTTCATG GGAAGATAGC TGTGGCACAT
AATGGCGAAT TGGTAAATGC TGCTCGATTA AGGAAAAAGC TTCTGCGTCA TGGTATTGGT
CTGTCTACAA GTTCTGATAG TGAAATGATT ACCCAGTTAC TGGCGTATAC CCCTCCTCAG
GAACAAGATG ACACCCCAGA CTGGGTAGCC AGGATTAAAA ACTTGATGAA GGAAGCACCC
ACAGCATACT CCCTGCTTAT AATGCACAGA GATGTTATTT ATGCAGTACG AGATCCTTAT
GGAAATCGTC CCTTATGCAT TGGTCGTCTT ATTCCAGTGT CTGATATAAA TGACAAAGAG
AAAAAAACAT CAGAAACAGA AGGATGGGTG GTGTCTTCAG AATCTTGTAG CTTCTTATCT
ATTGGTGCAA GATATTACCG TGAAGTCTTG CCTGGAGAAA TTGTGGAAAT ATCCAGACAC
AATGTCCAAA CTCTTGATAT TATATCAAGG TCTGAAGGAA ACCCAGTGGC TTTTTGTATC
TTTGAATATG TTTATTTTGC AAGACCAGAC AGTATGTTCG AAGACCAAAT GGTTTATACA
GTAAGATACC GTTGTGGCCA GCAGCTAGCG ATTGAAGCAC CTGTGGATGC AGATTTGGTT
AGCACTGTTC CAGAATCTGC TACGCCTGCT GCTCTTGCTT ACGCAGGAAA GTGTGGACTT
CCATATGTGG AGGTGCTGTG TAAAAACCGG TATGTAGGGA GAACCTTCAT TCAGCCAAAC
ATGAGGTTAA GACAACTTGG TGTTGCAAAA AAATTTGGAG TATTGTCAGA CAACTTTAAA
GGCAAAAGAA TTGTTCTTGT AGATGATTCA ATTGTCAGAG GCAATACCAT CTCACCTATA
ATAAAACTGC TCAAAGAATC TGGTGCAAAA GAGGTACACA TTCGAGTAGC TTCACCACCA
ATTAAATATC CATGCTTCAT GGGAATAAAC ATTCCTACAA AAGAAGAGCT CATTGCCAAT
AAACCAGAAT TTGATCACCT TGCAGAATAT CTAGGAGCAA ACAGTGTTGT GTATCTGTCA
GTAGAAGGAC TGGTTTCATC TGTACAAGAA GGGATAAAGT TTAAAAAACA GAAAGAGAAA
AAGCACGATA TTATGATCCA AGAAAATGGA AATGGTCTGG AATGTTTTGA AAAGAGTGGT
CATTGTACAG CTTGTCTCAC TGGAAAATAT CCTGTAGAAT TAGAATGGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011532689.1
CDS180..1490
Misc Feature(1)207..1481(+)
Misc Feature(2)207..821(+)
Misc Feature(3)213..377(+)
Misc Feature(4)852..1262(+)
Misc Feature(5)909..1202(+)
Translation
Position Chain Variation Link
1 1 a, c dbSNP:778956686
2 2 a, c dbSNP:754845652
4 4 g, t dbSNP:753664851
5 5 a, g dbSNP:765989683
9 9 c, t dbSNP:755787806
15 15 g, t dbSNP:375080631
24 24 a, g dbSNP:573844517
43 43 c, t dbSNP:761246399
64 64 c, t dbSNP:774868196
73 73 a, c dbSNP:370765017
76 76 c, t dbSNP:142572514
78 78 c, t dbSNP:775798687
81 81 g, t dbSNP:769973255
91 91 c, t dbSNP:569325646
96 96 a, c dbSNP:776654985
114 114 c, g, t dbSNP:555078164
121 121 c, t dbSNP:779046474
130 130 c, g dbSNP:754936603
131 131 g, t dbSNP:749162002
145 145 c, g dbSNP:779886575
174 174 c, g, t dbSNP:757843468
175 175 c, t dbSNP:559129421
180 180 a, g dbSNP:144550932
183 183 c, g dbSNP:142893232
186 186 a, g dbSNP:754125477
189 189 c, t dbSNP:200714501
190 190 a, c, g dbSNP:11538098
194 194 a, c dbSNP:147241535
207 207 c, t dbSNP:143863963
221 221 a, c dbSNP:776490775
226 226 a, c dbSNP:150840139
244 244 a, g dbSNP:557676402
260 260 c, t dbSNP:777453865
261 261 a, g dbSNP:141469911
276 276 c, t dbSNP:747764685
281 281 a, g dbSNP:778274879
289 289 c, t dbSNP:754454024
302 302 c, t dbSNP:749766197
305 305 c, t dbSNP:780665582
312 312 a, g dbSNP:756567329
315 315 a, t dbSNP:750794128
338 338 a, g dbSNP:767891813
346 346 a, g dbSNP:554702793
377 377 c, t dbSNP:748847830
385 385 c, t dbSNP:775135491
392 392 c, t dbSNP:368701517
404 404 a, g dbSNP:746387194
412 412 -, ctcc dbSNP:776294140
418 418 a, g dbSNP:781673559
426 426 c, g dbSNP:570611173
433 433 c, t dbSNP:771228885
434 434 c, t dbSNP:747379632
454 454 c, t dbSNP:775221186
478 478 c, g dbSNP:148624264
485 485 a, g dbSNP:759073359
488 488 c, t dbSNP:777023644
491 491 a, c dbSNP:771439982
506 506 c, t dbSNP:372357103
507 507 a, g dbSNP:538938687
529 529 a, g dbSNP:772368910
539 539 c, t dbSNP:748374814
564 564 c, t dbSNP:368322366
565 565 a, c, g dbSNP:374359651
573 573 c, t dbSNP:781029801
578 578 g, t dbSNP:757213321
584 584 c, t dbSNP:751368413
587 587 a, c dbSNP:148350001
593 593 c, t dbSNP:762766172
602 602 a, g dbSNP:575435588
607 607 c, t dbSNP:558853131
617 617 a, g, t dbSNP:2271924
635 635 c, t dbSNP:773791679
642 642 a, t dbSNP:767991634
660 660 a, c dbSNP:762207633
670 670 a, g dbSNP:758557747
674 674 c, t dbSNP:768077442
704 704 a, g dbSNP:762293406
716 716 a, t dbSNP:774780538
725 725 c, t dbSNP:764524347
751 751 c, g dbSNP:763183558
766 766 c, t dbSNP:113135347
815 815 a, g dbSNP:201692437
818 818 c, t dbSNP:769882717
823 823 a, g dbSNP:759853363
836 836 c, t dbSNP:776928042
837 837 a, g dbSNP:771166556
839 839 a, g dbSNP:551848333
847 847 a, t dbSNP:774483320
849 849 c, t dbSNP:768450806
852 852 g, t dbSNP:749176231
869 869 a, g dbSNP:779698922
871 871 c, t dbSNP:115979484
880 880 c, g, t dbSNP:780779350
890 890 a, g dbSNP:138492190
905 905 c, t dbSNP:145906060
923 923 a, g dbSNP:149024503
941 941 c, t dbSNP:77101789
951 951 c, t dbSNP:779428104
954 954 a, g dbSNP:369370347
962 962 a, g dbSNP:35420817
971 971 a, g dbSNP:766776716
972 972 a, g dbSNP:756393602
988 988 a, g dbSNP:750597436
993 993 a, g dbSNP:767496025
1019 1019 c, t dbSNP:112406471
1020 1020 a, g dbSNP:761909147
1024 1024 g, t dbSNP:775307283
1025 1025 a, g dbSNP:765137503
1028 1028 a, g dbSNP:759366007
1047 1047 a, g dbSNP:776405602
1048 1048 a, g dbSNP:770625597
1072 1072 a, g dbSNP:746648214
1076 1076 a, t dbSNP:772882442
1079 1079 a, g dbSNP:114731967
1097 1097 c, t dbSNP:375281372
1110 1110 a, g dbSNP:550124638
1137 1137 a, g dbSNP:755540789
1138 1138 c, t dbSNP:749731199
1140 1140 a, c dbSNP:780581720
1155 1155 g, t dbSNP:756477441
1166 1166 a, c dbSNP:372969235
1173 1173 a, g dbSNP:770028075
1186 1186 c, t dbSNP:761300467
1211 1211 a, t dbSNP:773988670
1236 1236 a, g dbSNP:768225373
1242 1242 a, g dbSNP:749814871
1275 1275 c, g dbSNP:780663607
1277 1277 c, t dbSNP:770073845
1295 1295 a, t dbSNP:560199458
1310 1310 a, g dbSNP:768309189
1316 1316 a, g dbSNP:762500968
1323 1323 a, g dbSNP:776183713
1329 1329 c, t dbSNP:527367886
1337 1337 a, t dbSNP:746284278
1343 1343 a, g dbSNP:2412738
1347 1347 c, g dbSNP:781606392
1352 1352 g, t dbSNP:76674380
1356 1356 a, c dbSNP:3908150
1362 1362 a, g dbSNP:147822472
1376 1376 c, g dbSNP:777924628
1384 1384 a, g dbSNP:758522806
1385 1385 c, t dbSNP:748196939
1386 1386 a, g dbSNP:779926163
1398 1398 c, t dbSNP:2660744
1402 1402 a, g dbSNP:756070370
1403 1403 a, c dbSNP:375085596
1414 1414 c, g dbSNP:767352428
1432 1432 a, c dbSNP:756959976
1433 1433 c, g dbSNP:751276884
1435 1435 a, g dbSNP:763812529
1440 1440 a, c dbSNP:144451574
1481 1481 a, g dbSNP:371980582
1484 1484 a, t dbSNP:200268394
1496 1496 a, c dbSNP:765722203
1501 1501 c, t dbSNP:760100296
1508 1508 c, g dbSNP:776933893
1516 1516 g, t dbSNP:771350079
1522 1522 c, t dbSNP:747354320
1524 1524 -, g dbSNP:771504957
1527 1527 a, g dbSNP:773463064
1538 1538 -, aag dbSNP:745778686
1559 1559 a, g dbSNP:113509123
1600 1600 c, t dbSNP:186916603
1607 1607 -, tttacc dbSNP:3036860
1611 1611 -, ccttta dbSNP:199711867
1634 1634 -, t dbSNP:34605681
1635 1635 -, t dbSNP:554346726
1638 1638 g, t dbSNP:199983276
1652 1652 a, c dbSNP:184074077
1657 1657 a, g dbSNP:777605224
1662 1662 c, t dbSNP:527429338
1768 1768 a, g dbSNP:559984575
1774 1774 c, g dbSNP:3733326
1782 1782 c, t dbSNP:566483988
1792 1792 a, g dbSNP:138098736
1806 1806 a, g dbSNP:536315832
1832 1832 a, g dbSNP:567402783
1884 1884 a, g dbSNP:550686805
1943 1943 a, c dbSNP:147000512
1961 1961 a, c dbSNP:562579960
1974 1974 a, t dbSNP:552405873
1977 1977 -, taataat dbSNP:746744741
1977 1977 -, taat dbSNP:761788458
1977 1977 a, t dbSNP:1042037
1977 1977 -, t dbSNP:200162900
1980 1980 -, taat dbSNP:376877956
1980 1980 a, t dbSNP:75801826
1980 1980 -, t dbSNP:34737334
1981 1981 -, aataa dbSNP:777889397
1983 1983 -, t, ta dbSNP:371730196
1983 1983 a, t dbSNP:78397688
1983 1983 -, t dbSNP:35057235
1984 1984 -, aat dbSNP:201291539
1984 1984 -, aa dbSNP:763765080
1985 1985 -, a dbSNP:200547738
2027 2027 -, attat dbSNP:199954490
2031 2031 -, ataa dbSNP:527733534
2036 2036 -, taat dbSNP:77344473
2036 2036 g, t dbSNP:191026688
2039 2039 -, taa dbSNP:200020195
2047 2047 c, t dbSNP:10000147
2057 2057 c, t dbSNP:551544425
2092 2092 a, t dbSNP:752854174
2146 2146 c, t dbSNP:143039832
2168 2168 a, c dbSNP:560962914
2207 2207 a, g dbSNP:111673999
2277 2277 a, c dbSNP:781428819
2280 2280 a, t dbSNP:746969202
2386 2386 -, c dbSNP:34557332
2400 2400 -, taatt dbSNP:562715054
2462 2462 -, t dbSNP:544520605
2473 2473 a, g dbSNP:751640671
2483 2483 a, g dbSNP:766880174
2525 2525 a, g dbSNP:758015770
2661 2661 g, t dbSNP:185325670
2688 2688 c, t dbSNP:180706331
2710 2710 c, g dbSNP:1042040
2729 2729 a, g dbSNP:539293873
2742 2742 a, t dbSNP:1062466
2752 2752 a, t dbSNP:146657767
2820 2820 c, t dbSNP:553282789
2841 2841 a, c dbSNP:188743398
2846 2846 -, ttgt dbSNP:571739574
2853 2853 c, t dbSNP:186476270
2866 2866 c, g dbSNP:140985493
2889 2889 a, g dbSNP:750952191
2892 2892 c, g dbSNP:557415803
2918 2918 a, g dbSNP:558955793
2920 2920 c, t dbSNP:537157831
2932 2932 a, g dbSNP:181233999
2962 2962 c, g, t dbSNP:754518266
3008 3008 c, t dbSNP:115400038
3009 3009 a, g dbSNP:17086746
3036 3036 c, t dbSNP:376970568
3055 3055 c, t dbSNP:566750737
3100 3100 a, c dbSNP:372821097
3130 3130 a, g dbSNP:546962801
3152 3152 a, g dbSNP:3796548
3201 3201 c, t dbSNP:773744962
3217 3217 a, t dbSNP:767969352
3231 3231 c, g dbSNP:560877385
3241 3241 a, c dbSNP:544251014
3287 3287 a, t dbSNP:771896194
3293 3293 -, t dbSNP:397739301
3301 3301 -, t dbSNP:3839138
3302 3302 -, t, tt dbSNP:554743177
3307 3307 a, g dbSNP:530543316
3343 3343 a, g dbSNP:565445785
3360 3360 a, t dbSNP:773797257
3401 3401 c, t dbSNP:545259724
3419 3419 c, g dbSNP:573127103

Target ORF information:

RefSeq Version XM_011534387
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphoribosyl pyrophosphate amidotransferase (PPAT), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011534387

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGGAGTTC GGTGCCAACA TTCAAATCAC ACAAGGTATG CCACCACAGG AAAATGTGAA 
CTAGAAAATT GTCAGCCCTT CGTTGTTGAA ACACTTCATG GGAAGATAGC TGTGGCACAT
AATGGCGAAT TGGTAAATGC TGCTCGATTA AGGAAAAAGC TTCTGCGTCA TGGTATTGGT
CTGTCTACAA GTTCTGATAG TGAAATGATT ACCCAGTTAC TGGCGTATAC CCCTCCTCAG
GAACAAGATG ACACCCCAGA CTGGGTAGCC AGGATTAAAA ACTTGATGAA GGAAGCACCC
ACAGCATACT CCCTGCTTAT AATGCACAGA GATGTTATTT ATGCAGTACG AGATCCTTAT
GGAAATCGTC CCTTATGCAT TGGTCGTCTT ATTCCAGTGT CTGATATAAA TGACAAAGAG
AAAAAAACAT CAGAAACAGA AGGATGGGTG GTGTCTTCAG AATCTTGTAG CTTCTTATCT
ATTGGTGCAA GATATTACCG TGAAGTCTTG CCTGGAGAAA TTGTGGAAAT ATCCAGACAC
AATGTCCAAA CTCTTGATAT TATATCAAGG TCTGAAGGAA ACCCAGTGGC TTTTTGTATC
TTTGAATATG TTTATTTTGC AAGACCAGAC AGTATGTTCG AAGACCAAAT GGTTTATACA
GTAAGATACC GTTGTGGCCA GCAGCTAGCG ATTGAAGCAC CTGTGGATGC AGATTTGGTT
AGCACTGTTC CAGAATCTGC TACGCCTGCT GCTCTTGCTT ACGCAGGAAA GTGTGGACTT
CCATATGTGG AGGTGCTGTG TAAAAACCGG TATGTAGGGA GAACCTTCAT TCAGCCAAAC
ATGAGGTTAA GACAACTTGG TGTTGCAAAA AAATTTGGAG TATTGTCAGA CAACTTTAAA
GGCAAAAGAA TTGTTCTTGT AGATGATTCA ATTGTCAGAG GCAATACCAT CTCACCTATA
ATAAAACTGC TCAAAGAATC TGGTGCAAAA GAGGTACACA TTCGAGTAGC TTCACCACCA
ATTAAATATC CATGCTTCAT GGGAATAAAC ATTCCTACAA AAGAAGAGCT CATTGCCAAT
AAACCAGAAT TTGATCACCT TGCAGAATAT CTAGGAGCAA ACAGTGTTGT GTATCTGTCA
GTAGAAGGAC TGGTTTCATC TGTACAAGAA GGGATAAAGT TTAAAAAACA GAAAGAGAAA
AAGCACGATA TTATGATCCA AGAAAATGGA AATGGTCTGG AATGTTTTGA AAAGAGTGGT
CATTGTACAG CTTGTCTCAC TGGAAAATAT CCTGTAGAAT TAGAATGGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03961
Accession Version NM_002703.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1554bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product amidophosphoribosyltransferase proprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB039655.1, BC004200.2, AB209246.1 and U00238.1. On Mar 4, 2011 this sequence version replaced gi:29570797. Summary: The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq, Mar 2011]. ##Evidence-Data-START## Transcript exon combination :: U00238.1, D13757.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGAGCTGG AGGAGTTGGG GATCCGAGAG GAATGTGGCG TGTTCGGGTG CATCGCCTCA 
GGAGAGTGGC CCACGCAGCT GGATGTACCG CATGTGATCA CTCTGGGACT CGTGGGGCTG
CAGCACCGGG GTCAGGAGAG TGCTGGTATT GTGACTAGTG ATGGGAGTTC GGTGCCAACA
TTCAAATCAC ACAAGGGAAT GGGTCTTGTA AATCACGTCT TTACTGAAGA CAATTTGAAA
AAATTATATG TTTCAAATCT TGGAATTGGA CACACCAGGT ATGCCACCAC AGGAAAATGT
GAACTAGAAA ATTGTCAGCC CTTCGTTGTT GAAACACTTC ATGGGAAGAT AGCTGTGGCA
CATAATGGCG AATTGGTAAA TGCTGCTCGA TTAAGGAAAA AGCTTCTGCG TCATGGTATT
GGTCTGTCTA CAAGTTCTGA TAGTGAAATG ATTACCCAGT TACTGGCGTA TACCCCTCCT
CAGGAACAAG ATGACACCCC AGACTGGGTA GCCAGGATTA AAAACTTGAT GAAGGAAGCA
CCCACAGCAT ACTCCCTGCT TATAATGCAC AGAGATGTTA TTTATGCAGT ACGAGATCCT
TATGGAAATC GTCCCTTATG CATTGGTCGT CTTATTCCAG TGTCTGATAT AAATGACAAA
GAGAAAAAAA CATCAGAAAC AGAAGGATGG GTGGTGTCTT CAGAATCTTG TAGCTTCTTA
TCTATTGGTG CAAGATATTA CCGTGAAGTC TTGCCTGGAG AAATTGTGGA AATATCCAGA
CACAATGTCC AAACTCTTGA TATTATATCA AGGTCTGAAG GAAACCCAGT GGCTTTTTGT
ATCTTTGAAT ATGTTTATTT TGCAAGACCA GACAGTATGT TCGAAGACCA AATGGTTTAT
ACAGTAAGAT ACCGTTGTGG CCAGCAGCTA GCGATTGAAG CACCTGTGGA TGCAGATTTG
GTTAGCACTG TTCCAGAATC TGCTACGCCT GCTGCTCTTG CTTACGCAGG AAAGTGTGGA
CTTCCATATG TGGAGGTGCT GTGTAAAAAC CGGTATGTAG GGAGAACCTT CATTCAGCCA
AACATGAGGT TAAGACAACT TGGTGTTGCA AAAAAATTTG GAGTATTGTC AGACAACTTT
AAAGGCAAAA GAATTGTTCT TGTAGATGAT TCAATTGTCA GAGGCAATAC CATCTCACCT
ATAATAAAAC TGCTCAAAGA ATCTGGTGCA AAAGAGGTAC ACATTCGAGT AGCTTCACCA
CCAATTAAAT ATCCATGCTT CATGGGAATA AACATTCCTA CAAAAGAAGA GCTCATTGCC
AATAAACCAG AATTTGATCA CCTTGCAGAA TATCTAGGAG CAAACAGTGT TGTGTATCTG
TCAGTAGAAG GACTGGTTTC ATCTGTACAA GAAGGGATAA AGTTTAAAAA ACAGAAAGAG
AAAAAGCACG ATATTATGAT CCAAGAAAAT GGAAATGGTC TGGAATGTTT TGAAAAGAGT
GGTCATTGTA CAGCTTGTCT CACTGGAAAA TATCCTGTAG AATTAGAATG GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_002694.3
CDS160..1713
Misc Feature(1)160..162(+)
Misc Feature(2)181..1704(+)
Misc Feature(3)193..1044(+)
Misc Feature(4)193..600(+)
Misc Feature(5)259..630(+)
Misc Feature(6)1075..1485(+)
Misc Feature(7)1132..1425(+)
Exon (1)1..287
Gene:PPAT
Gene Synonym:
Exon (2)288..354
Gene:PPAT
Gene Synonym:
Exon (3)355..561
Gene:PPAT
Gene Synonym:
Exon (4)562..674
Gene:PPAT
Gene Synonym:
Exon (5)675..820
Gene:PPAT
Gene Synonym:
Exon (6)821..893
Gene:PPAT
Gene Synonym:
Exon (7)894..1045
Gene:PPAT
Gene Synonym:
Exon (8)1046..1173
Gene:PPAT
Gene Synonym:
Exon (9)1174..1395
Gene:PPAT
Gene Synonym:
Exon (10)1396..1516
Gene:PPAT
Gene Synonym:
Exon (11)1517..3703
Gene:PPAT
Gene Synonym:
Translation
Position Chain Variation Link
11 11 c, t dbSNP:572353430
15 15 c, t dbSNP:774980557
27 27 c, g dbSNP:758097056
35 35 a, g dbSNP:60112184
43 43 c, g dbSNP:541461683
52 52 a, g dbSNP:575699835
53 53 c, t dbSNP:3822001
67 67 c, g dbSNP:59010837
70 70 c, t dbSNP:60097571
84 84 c, g dbSNP:554389201
88 88 a, c dbSNP:189152163
89 89 a, g dbSNP:375418740
108 108 g, t dbSNP:772747599
109 109 c, g dbSNP:201978310
118 118 g, t dbSNP:58548914
122 122 c, t dbSNP:6843073
123 123 g, t dbSNP:769011905
127 127 c, t dbSNP:540988880
128 128 g, t dbSNP:749612783
133 133 a, g dbSNP:755376801
134 134 g, t dbSNP:202181751
135 135 a, c, g dbSNP:745970882
138 138 g, t dbSNP:781324117
139 139 c, t dbSNP:757230006
140 140 c, t dbSNP:752689448
141 141 a, c dbSNP:778956686
142 142 a, c dbSNP:754845652
144 144 g, t dbSNP:753664851
145 145 a, g dbSNP:765989683
149 149 c, t dbSNP:755787806
155 155 g, t dbSNP:375080631
164 164 a, g dbSNP:573844517
183 183 c, t dbSNP:761246399
204 204 c, t dbSNP:774868196
213 213 a, c dbSNP:370765017
216 216 c, t dbSNP:142572514
218 218 c, t dbSNP:775798687
221 221 g, t dbSNP:769973255
231 231 c, t dbSNP:569325646
236 236 a, c dbSNP:776654985
254 254 c, g, t dbSNP:555078164
261 261 c, t dbSNP:779046474
270 270 c, g dbSNP:754936603
271 271 g, t dbSNP:749162002
285 285 c, g dbSNP:779886575
314 314 c, g, t dbSNP:757843468
315 315 c, t dbSNP:559129421
320 320 a, g dbSNP:144550932
323 323 c, g dbSNP:142893232
326 326 a, g dbSNP:754125477
329 329 c, t dbSNP:200714501
330 330 a, c, g dbSNP:11538098
334 334 a, c dbSNP:147241535
347 347 c, t dbSNP:143863963
375 375 c, t dbSNP:761055747
381 381 -, t dbSNP:765350710
392 392 a, g dbSNP:750703999
405 405 a, g dbSNP:200923724
407 407 -, at dbSNP:757548852
408 408 c, t dbSNP:138162667
414 414 a, c, g dbSNP:774263224
433 433 a, g dbSNP:769833390
435 435 c, g dbSNP:759373880
444 444 a, c dbSNP:776490775
449 449 a, c dbSNP:150840139
467 467 a, g dbSNP:557676402
483 483 c, t dbSNP:777453865
484 484 a, g dbSNP:141469911
499 499 c, t dbSNP:747764685
504 504 a, g dbSNP:778274879
512 512 c, t dbSNP:754454024
525 525 c, t dbSNP:749766197
528 528 c, t dbSNP:780665582
535 535 a, g dbSNP:756567329
538 538 a, t dbSNP:750794128
561 561 a, g dbSNP:767891813
569 569 a, g dbSNP:554702793
600 600 c, t dbSNP:748847830
608 608 c, t dbSNP:775135491
615 615 c, t dbSNP:368701517
627 627 a, g dbSNP:746387194
635 635 -, ctcc dbSNP:776294140
641 641 a, g dbSNP:781673559
649 649 c, g dbSNP:570611173
656 656 c, t dbSNP:771228885
657 657 c, t dbSNP:747379632
677 677 c, t dbSNP:775221186
701 701 c, g dbSNP:148624264
708 708 a, g dbSNP:759073359
711 711 c, t dbSNP:777023644
714 714 a, c dbSNP:771439982
729 729 c, t dbSNP:372357103
730 730 a, g dbSNP:538938687
752 752 a, g dbSNP:772368910
762 762 c, t dbSNP:748374814
787 787 c, t dbSNP:368322366
788 788 a, c, g dbSNP:374359651
796 796 c, t dbSNP:781029801
801 801 g, t dbSNP:757213321
807 807 c, t dbSNP:751368413
810 810 a, c dbSNP:148350001
816 816 c, t dbSNP:762766172
825 825 a, g dbSNP:575435588
830 830 c, t dbSNP:558853131
840 840 a, g, t dbSNP:2271924
858 858 c, t dbSNP:773791679
865 865 a, t dbSNP:767991634
883 883 a, c dbSNP:762207633
893 893 a, g dbSNP:758557747
897 897 c, t dbSNP:768077442
927 927 a, g dbSNP:762293406
939 939 a, t dbSNP:774780538
948 948 c, t dbSNP:764524347
974 974 c, g dbSNP:763183558
989 989 c, t dbSNP:113135347
1038 1038 a, g dbSNP:201692437
1041 1041 c, t dbSNP:769882717
1046 1046 a, g dbSNP:759853363
1059 1059 c, t dbSNP:776928042
1060 1060 a, g dbSNP:771166556
1062 1062 a, g dbSNP:551848333
1070 1070 a, t dbSNP:774483320
1072 1072 c, t dbSNP:768450806
1075 1075 g, t dbSNP:749176231
1092 1092 a, g dbSNP:779698922
1094 1094 c, t dbSNP:115979484
1103 1103 c, g, t dbSNP:780779350
1113 1113 a, g dbSNP:138492190
1128 1128 c, t dbSNP:145906060
1146 1146 a, g dbSNP:149024503
1164 1164 c, t dbSNP:77101789
1174 1174 c, t dbSNP:779428104
1177 1177 a, g dbSNP:369370347
1185 1185 a, g dbSNP:35420817
1194 1194 a, g dbSNP:766776716
1195 1195 a, g dbSNP:756393602
1211 1211 a, g dbSNP:750597436
1216 1216 a, g dbSNP:767496025
1242 1242 c, t dbSNP:112406471
1243 1243 a, g dbSNP:761909147
1247 1247 g, t dbSNP:775307283
1248 1248 a, g dbSNP:765137503
1251 1251 a, g dbSNP:759366007
1270 1270 a, g dbSNP:776405602
1271 1271 a, g dbSNP:770625597
1295 1295 a, g dbSNP:746648214
1299 1299 a, t dbSNP:772882442
1302 1302 a, g dbSNP:114731967
1320 1320 c, t dbSNP:375281372
1333 1333 a, g dbSNP:550124638
1360 1360 a, g dbSNP:755540789
1361 1361 c, t dbSNP:749731199
1363 1363 a, c dbSNP:780581720
1378 1378 g, t dbSNP:756477441
1389 1389 a, c dbSNP:372969235
1396 1396 a, g dbSNP:770028075
1409 1409 c, t dbSNP:761300467
1434 1434 a, t dbSNP:773988670
1459 1459 a, g dbSNP:768225373
1465 1465 a, g dbSNP:749814871
1498 1498 c, g dbSNP:780663607
1500 1500 c, t dbSNP:770073845
1518 1518 a, t dbSNP:560199458
1533 1533 a, g dbSNP:768309189
1539 1539 a, g dbSNP:762500968
1546 1546 a, g dbSNP:776183713
1552 1552 c, t dbSNP:527367886
1560 1560 a, t dbSNP:746284278
1566 1566 a, g dbSNP:2412738
1570 1570 c, g dbSNP:781606392
1575 1575 g, t dbSNP:76674380
1579 1579 a, c dbSNP:3908150
1585 1585 a, g dbSNP:147822472
1599 1599 c, g dbSNP:777924628
1607 1607 a, g dbSNP:758522806
1608 1608 c, t dbSNP:748196939
1609 1609 a, g dbSNP:779926163
1621 1621 c, t dbSNP:2660744
1625 1625 a, g dbSNP:756070370
1626 1626 a, c dbSNP:375085596
1637 1637 c, g dbSNP:767352428
1655 1655 a, c dbSNP:756959976
1656 1656 c, g dbSNP:751276884
1658 1658 a, g dbSNP:763812529
1663 1663 a, c dbSNP:144451574
1704 1704 a, g dbSNP:371980582
1707 1707 a, t dbSNP:200268394
1719 1719 a, c dbSNP:765722203
1724 1724 c, t dbSNP:760100296
1731 1731 c, g dbSNP:776933893
1739 1739 g, t dbSNP:771350079
1745 1745 c, t dbSNP:747354320
1747 1747 -, g dbSNP:771504957
1750 1750 a, g dbSNP:773463064
1761 1761 -, aag dbSNP:745778686
1782 1782 a, g dbSNP:113509123
1823 1823 c, t dbSNP:186916603
1830 1830 -, tttacc dbSNP:3036860
1834 1834 -, ccttta dbSNP:199711867
1857 1857 -, t dbSNP:34605681
1858 1858 -, t dbSNP:554346726
1861 1861 g, t dbSNP:199983276
1875 1875 a, c dbSNP:184074077
1880 1880 a, g dbSNP:777605224
1885 1885 c, t dbSNP:527429338
1991 1991 a, g dbSNP:559984575
1997 1997 c, g dbSNP:3733326
2005 2005 c, t dbSNP:566483988
2015 2015 a, g dbSNP:138098736
2029 2029 a, g dbSNP:536315832
2055 2055 a, g dbSNP:567402783
2107 2107 a, g dbSNP:550686805
2166 2166 a, c dbSNP:147000512
2184 2184 a, c dbSNP:562579960
2197 2197 a, t dbSNP:552405873
2200 2200 -, taataat dbSNP:746744741
2200 2200 -, taat dbSNP:761788458
2200 2200 a, t dbSNP:1042037
2200 2200 -, t dbSNP:200162900
2203 2203 -, taat dbSNP:376877956
2203 2203 a, t dbSNP:75801826
2203 2203 -, t dbSNP:34737334
2204 2204 -, aataa dbSNP:777889397
2206 2206 -, t, ta dbSNP:371730196
2206 2206 a, t dbSNP:78397688
2206 2206 -, t dbSNP:35057235
2207 2207 -, aat dbSNP:201291539
2207 2207 -, aa dbSNP:763765080
2208 2208 -, a dbSNP:200547738
2250 2250 -, attat dbSNP:199954490
2254 2254 -, ataa dbSNP:527733534
2259 2259 -, taat dbSNP:77344473
2259 2259 g, t dbSNP:191026688
2262 2262 -, taa dbSNP:200020195
2270 2270 c, t dbSNP:10000147
2280 2280 c, t dbSNP:551544425
2315 2315 a, t dbSNP:752854174
2369 2369 c, t dbSNP:143039832
2391 2391 a, c dbSNP:560962914
2430 2430 a, g dbSNP:111673999
2500 2500 a, c dbSNP:781428819
2503 2503 a, t dbSNP:746969202
2609 2609 -, c dbSNP:34557332
2623 2623 -, taatt dbSNP:562715054
2685 2685 -, t dbSNP:544520605
2696 2696 a, g dbSNP:751640671
2706 2706 a, g dbSNP:766880174
2748 2748 a, g dbSNP:758015770
2884 2884 g, t dbSNP:185325670
2911 2911 c, t dbSNP:180706331
2933 2933 c, g dbSNP:1042040
2952 2952 a, g dbSNP:539293873
2965 2965 a, t dbSNP:1062466
2975 2975 a, t dbSNP:146657767
3043 3043 c, t dbSNP:553282789
3064 3064 a, c dbSNP:188743398
3069 3069 -, ttgt dbSNP:571739574
3076 3076 c, t dbSNP:186476270
3089 3089 c, g dbSNP:140985493
3112 3112 a, g dbSNP:750952191
3115 3115 c, g dbSNP:557415803
3141 3141 a, g dbSNP:558955793
3143 3143 c, t dbSNP:537157831
3155 3155 a, g dbSNP:181233999
3185 3185 c, g, t dbSNP:754518266
3231 3231 c, t dbSNP:115400038
3232 3232 a, g dbSNP:17086746
3259 3259 c, t dbSNP:376970568
3278 3278 c, t dbSNP:566750737
3323 3323 a, c dbSNP:372821097
3353 3353 a, g dbSNP:546962801
3375 3375 a, g dbSNP:3796548
3424 3424 c, t dbSNP:773744962
3440 3440 a, t dbSNP:767969352
3454 3454 c, g dbSNP:560877385
3464 3464 a, c dbSNP:544251014
3510 3510 a, t dbSNP:771896194
3516 3516 -, t dbSNP:397739301
3524 3524 -, t dbSNP:3839138
3525 3525 -, t, tt dbSNP:554743177
3530 3530 a, g dbSNP:530543316
3566 3566 a, g dbSNP:565445785
3583 3583 a, t dbSNP:773797257
3624 3624 c, t dbSNP:545259724
3642 3642 c, g dbSNP:573127103

Target ORF information:

RefSeq Version NM_002703
Organism Homo sapiens (human)
Definition Homo sapiens phosphoribosyl pyrophosphate amidotransferase (PPAT), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002703

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGAGCTGG AGGAGTTGGG GATCCGAGAG GAATGTGGCG TGTTCGGGTG CATCGCCTCA 
GGAGAGTGGC CCACGCAGCT GGATGTACCG CATGTGATCA CTCTGGGACT CGTGGGGCTG
CAGCACCGGG GTCAGGAGAG TGCTGGTATT GTGACTAGTG ATGGGAGTTC GGTGCCAACA
TTCAAATCAC ACAAGGGAAT GGGTCTTGTA AATCACGTCT TTACTGAAGA CAATTTGAAA
AAATTATATG TTTCAAATCT TGGAATTGGA CACACCAGGT ATGCCACCAC AGGAAAATGT
GAACTAGAAA ATTGTCAGCC CTTCGTTGTT GAAACACTTC ATGGGAAGAT AGCTGTGGCA
CATAATGGCG AATTGGTAAA TGCTGCTCGA TTAAGGAAAA AGCTTCTGCG TCATGGTATT
GGTCTGTCTA CAAGTTCTGA TAGTGAAATG ATTACCCAGT TACTGGCGTA TACCCCTCCT
CAGGAACAAG ATGACACCCC AGACTGGGTA GCCAGGATTA AAAACTTGAT GAAGGAAGCA
CCCACAGCAT ACTCCCTGCT TATAATGCAC AGAGATGTTA TTTATGCAGT ACGAGATCCT
TATGGAAATC GTCCCTTATG CATTGGTCGT CTTATTCCAG TGTCTGATAT AAATGACAAA
GAGAAAAAAA CATCAGAAAC AGAAGGATGG GTGGTGTCTT CAGAATCTTG TAGCTTCTTA
TCTATTGGTG CAAGATATTA CCGTGAAGTC TTGCCTGGAG AAATTGTGGA AATATCCAGA
CACAATGTCC AAACTCTTGA TATTATATCA AGGTCTGAAG GAAACCCAGT GGCTTTTTGT
ATCTTTGAAT ATGTTTATTT TGCAAGACCA GACAGTATGT TCGAAGACCA AATGGTTTAT
ACAGTAAGAT ACCGTTGTGG CCAGCAGCTA GCGATTGAAG CACCTGTGGA TGCAGATTTG
GTTAGCACTG TTCCAGAATC TGCTACGCCT GCTGCTCTTG CTTACGCAGG AAAGTGTGGA
CTTCCATATG TGGAGGTGCT GTGTAAAAAC CGGTATGTAG GGAGAACCTT CATTCAGCCA
AACATGAGGT TAAGACAACT TGGTGTTGCA AAAAAATTTG GAGTATTGTC AGACAACTTT
AAAGGCAAAA GAATTGTTCT TGTAGATGAT TCAATTGTCA GAGGCAATAC CATCTCACCT
ATAATAAAAC TGCTCAAAGA ATCTGGTGCA AAAGAGGTAC ACATTCGAGT AGCTTCACCA
CCAATTAAAT ATCCATGCTT CATGGGAATA AACATTCCTA CAAAAGAAGA GCTCATTGCC
AATAAACCAG AATTTGATCA CCTTGCAGAA TATCTAGGAG CAAACAGTGT TGTGTATCTG
TCAGTAGAAG GACTGGTTTC ATCTGTACAA GAAGGGATAA AGTTTAAAAA ACAGAAAGAG
AAAAAGCACG ATATTATGAT CCAAGAAAAT GGAAATGGTC TGGAATGTTT TGAAAAGAGT
GGTCATTGTA CAGCTTGTCT CACTGGAAAA TATCCTGTAG AATTAGAATG GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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