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CTSA cDNA ORF clone, Homo sapiens (human)

Gene Symbol CTSA
Entrez Gene ID 5476
Full Name cathepsin A
Synonyms GLB2, GSL, NGBE, PPCA, PPGB
General protein information
Preferred Names
lysosomal protective protein
Names
lysosomal protective protein
deamidase
urinary kininase
carboxypeptidase C
carboxypeptidase L
carboxypeptidase-L
beta-galactosidase 2
lysosomal carboxypeptidase A
protective protein cathepsin A
carboxypeptidase Y-like kininase
beta-galactosidase protective protein
NP_000299.2
NP_001121167.1
NP_001161066.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

20

20q13.1

Summary This gene encodes a glycoprotein which associates with lysosomal enzymes beta-galactosidase and neuraminidase to form a complex of high molecular weight multimers. The formation of this complex provides a protective role for stability and activity. Deficiencies in this gene are linked to multiple forms of galactosialidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

613111

Disorder Html: Galactosialidosis, 256540 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000308 NP_000299 lysosomal protective protein isoform a precursor
NM_001127695 NP_001121167 lysosomal protective protein isoform b precursor
NM_001167594 NP_001161066 lysosomal protective protein isoform c precursor



Homo sapiens (human) CTSA NP_000299.2
Pan troglodytes (chimpanzee) CTSA XP_001159669.2
Macaca mulatta (Rhesus monkey) CTSA XP_001106032.1
Canis lupus familiaris (dog) CTSA NP_001103385.1
Bos taurus (cattle) CTSA NP_001030403.1
Mus musculus (house mouse) Ctsa NP_032932.2
Rattus norvegicus (Norway rat) Ctsa NP_001011959.1
Gallus gallus (chicken) CTSA NP_001026662.1
Danio rerio (zebrafish) ctsa NP_956844.1
Caenorhabditis elegans F13D12.6 NP_496507.1
Caenorhabditis elegans F41C3.5 NP_494846.1
Arabidopsis thaliana (thale cress) scpl20 NP_193027.5
Arabidopsis thaliana (thale cress) scpl21 NP_189169.1
Xenopus (Silurana) tropicalis (western clawed frog) ctsa NP_001039207.1


Related articles in PubMed

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CTSA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CTSA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu17877
NM_000308 Homo sapiens cathepsin A (CTSA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$209.50-$293.30
$419.00
OHu06757 NM_001127695 Homo sapiens cathepsin A (CTSA), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00
OHu04054 NM_001167594 Homo sapiens cathepsin A (CTSA), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu17877
Clone ID Related Accession (Same CDS sequence) NM_000308
Accession Version NM_000308.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1497bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product lysosomal protective protein isoform a precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA029446.1 and BC093009.1. This sequence is a reference standard in the RefSeqGene project. On Dec 14, 2006 this sequence version replaced gi:4505988. Transcript Variant: This variant (1) encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC093009.1, AB209705.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACTTCCA GTCCCCGGGC GCCTCCTGGA GAGCAAGGAC GCGGGGGAGC AGAGATGATC 
CGAGCCGCGC CGCCGCCGCT GTTCCTGCTG CTGCTGCTGC TGCTGCTGCT AGTGTCCTGG
GCGTCCCGAG GCGAGGCAGC CCCCGACCAG GACGAGATCC AGCGCCTCCC CGGGCTGGCC
AAGCAGCCGT CTTTCCGCCA GTACTCCGGC TACCTCAAAG GCTCCGGCTC CAAGCACCTC
CACTACTGGT TTGTGGAGTC CCAGAAGGAT CCCGAGAACA GCCCTGTGGT GCTTTGGCTC
AATGGGGGTC CCGGCTGCAG CTCACTAGAT GGGCTCCTCA CAGAGCATGG CCCCTTCCTG
GTCCAGCCAG ATGGTGTCAC CCTGGAGTAC AACCCCTATT CTTGGAATCT GATTGCCAAT
GTGTTATACC TGGAGTCCCC AGCTGGGGTG GGCTTCTCCT ACTCCGATGA CAAGTTTTAT
GCAACTAATG ACACTGAGGT CGCCCAGAGC AATTTTGAGG CCCTTCAAGA TTTCTTCCGC
CTCTTTCCGG AGTACAAGAA CAACAAACTT TTCCTGACCG GGGAGAGCTA TGCTGGCATC
TACATCCCCA CCCTGGCCGT GCTGGTCATG CAGGATCCCA GCATGAACCT TCAGGGGCTG
GCTGTGGGCA ATGGACTCTC CTCCTATGAG CAGAATGACA ACTCCCTGGT CTACTTTGCC
TACTACCATG GCCTTCTGGG GAACAGGCTT TGGTCTTCTC TCCAGACCCA CTGCTGCTCT
CAAAACAAGT GTAACTTCTA TGACAACAAA GACCTGGAAT GCGTGACCAA TCTTCAGGAA
GTGGCCCGCA TCGTGGGCAA CTCTGGCCTC AACATCTACA ATCTCTATGC CCCGTGTGCT
GGAGGGGTGC CCAGCCATTT TAGGTATGAG AAGGACACTG TTGTGGTCCA GGATTTGGGC
AACATCTTCA CTCGCCTGCC ACTCAAGCGG ATGTGGCATC AGGCACTGCT GCGCTCAGGG
GATAAAGTGC GCATGGACCC CCCCTGCACC AACACAACAG CTGCTTCCAC CTACCTCAAC
AACCCGTACG TGCGGAAGGC CCTCAACATC CCGGAGCAGC TGCCACAATG GGACATGTGC
AACTTTCTGG TAAACTTACA GTACCGCCGT CTCTACCGAA GCATGAACTC CCAGTATCTG
AAGCTGCTTA GCTCACAGAA ATACCAGATC CTATTATATA ATGGAGATGT AGACATGGCC
TGCAATTTCA TGGGGGATGA GTGGTTTGTG GATTCCCTCA ACCAGAAGAT GGAGGTGCAG
CGCCGGCCCT GGTTAGTGAA GTACGGGGAC AGCGGGGAGC AGATTGCCGG CTTCGTGAAG
GAGTTCTCCC ACATCGCCTT TCTCACGATC AAGGGCGCCG GCCACATGGT TCCCACCGAC
AAGCCCCTCG CTGCCTTCAC CATGTTCTCC CGCTTCCTGA ACAAGCAGCC ATACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000299.2
CDS375..1871
Misc Feature(1)363..365(+)
Misc Feature(2)543..1856(+)
Exon (1)1..428
Gene:CTSA
Gene Synonym:
Exon (2)429..622
Gene:CTSA
Gene Synonym:
Exon (3)623..734
Gene:CTSA
Gene Synonym:
Exon (4)735..785
Gene:CTSA
Gene Synonym:
Exon (5)786..872
Gene:CTSA
Gene Synonym:
Exon (6)873..1028
Gene:CTSA
Gene Synonym:
Exon (7)1029..1120
Gene:CTSA
Gene Synonym:
Exon (8)1121..1205
Gene:CTSA
Gene Synonym:
Exon (9)1206..1297
Gene:CTSA
Gene Synonym:
Exon (10)1298..1376
Gene:CTSA
Gene Synonym:
Exon (11)1377..1516
Gene:CTSA
Gene Synonym:
Exon (12)1517..1592
Gene:CTSA
Gene Synonym:
Exon (13)1593..1682
Gene:CTSA
Gene Synonym:
Exon (14)1683..1787
Gene:CTSA
Gene Synonym:
Exon (15)1788..2241
Gene:CTSA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000308
Organism Homo sapiens (human)
Definition Homo sapiens cathepsin A (CTSA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000308

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACTTCCA GTCCCCGGGC GCCTCCTGGA GAGCAAGGAC GCGGGGGAGC AGAGATGATC 
CGAGCCGCGC CGCCGCCGCT GTTCCTGCTG CTGCTGCTGC TGCTGCTGCT AGTGTCCTGG
GCGTCCCGAG GCGAGGCAGC CCCCGACCAG GACGAGATCC AGCGCCTCCC CGGGCTGGCC
AAGCAGCCGT CTTTCCGCCA GTACTCCGGC TACCTCAAAG GCTCCGGCTC CAAGCACCTC
CACTACTGGT TTGTGGAGTC CCAGAAGGAT CCCGAGAACA GCCCTGTGGT GCTTTGGCTC
AATGGGGGTC CCGGCTGCAG CTCACTAGAT GGGCTCCTCA CAGAGCATGG CCCCTTCCTG
GTCCAGCCAG ATGGTGTCAC CCTGGAGTAC AACCCCTATT CTTGGAATCT GATTGCCAAT
GTGTTATACC TGGAGTCCCC AGCTGGGGTG GGCTTCTCCT ACTCCGATGA CAAGTTTTAT
GCAACTAATG ACACTGAGGT CGCCCAGAGC AATTTTGAGG CCCTTCAAGA TTTCTTCCGC
CTCTTTCCGG AGTACAAGAA CAACAAACTT TTCCTGACCG GGGAGAGCTA TGCTGGCATC
TACATCCCCA CCCTGGCCGT GCTGGTCATG CAGGATCCCA GCATGAACCT TCAGGGGCTG
GCTGTGGGCA ATGGACTCTC CTCCTATGAG CAGAATGACA ACTCCCTGGT CTACTTTGCC
TACTACCATG GCCTTCTGGG GAACAGGCTT TGGTCTTCTC TCCAGACCCA CTGCTGCTCT
CAAAACAAGT GTAACTTCTA TGACAACAAA GACCTGGAAT GCGTGACCAA TCTTCAGGAA
GTGGCCCGCA TCGTGGGCAA CTCTGGCCTC AACATCTACA ATCTCTATGC CCCGTGTGCT
GGAGGGGTGC CCAGCCATTT TAGGTATGAG AAGGACACTG TTGTGGTCCA GGATTTGGGC
AACATCTTCA CTCGCCTGCC ACTCAAGCGG ATGTGGCATC AGGCACTGCT GCGCTCAGGG
GATAAAGTGC GCATGGACCC CCCCTGCACC AACACAACAG CTGCTTCCAC CTACCTCAAC
AACCCGTACG TGCGGAAGGC CCTCAACATC CCGGAGCAGC TGCCACAATG GGACATGTGC
AACTTTCTGG TAAACTTACA GTACCGCCGT CTCTACCGAA GCATGAACTC CCAGTATCTG
AAGCTGCTTA GCTCACAGAA ATACCAGATC CTATTATATA ATGGAGATGT AGACATGGCC
TGCAATTTCA TGGGGGATGA GTGGTTTGTG GATTCCCTCA ACCAGAAGAT GGAGGTGCAG
CGCCGGCCCT GGTTAGTGAA GTACGGGGAC AGCGGGGAGC AGATTGCCGG CTTCGTGAAG
GAGTTCTCCC ACATCGCCTT TCTCACGATC AAGGGCGCCG GCCACATGGT TCCCACCGAC
AAGCCCCTCG CTGCCTTCAC CATGTTCTCC CGCTTCCTGA ACAAGCAGCC ATACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu06757
Clone ID Related Accession (Same CDS sequence) NM_001127695
Accession Version NM_001127695.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1443bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product lysosomal protective protein isoform b precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA029446.1, AK307701.1 and BC093009.1. Transcript Variant: This variant (2) has an alternate splice site in the 5' region, which results in a downstream AUG start codon, as compared to variant 1. The resulting isoform (b) has a shorter N-terminus, as compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000597.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGATCCGAG CCGCGCCGCC GCCGCTGTTC CTGCTGCTGC TGCTGCTGCT GCTGCTAGTG 
TCCTGGGCGT CCCGAGGCGA GGCAGCCCCC GACCAGGACG AGATCCAGCG CCTCCCCGGG
CTGGCCAAGC AGCCGTCTTT CCGCCAGTAC TCCGGCTACC TCAAAGGCTC CGGCTCCAAG
CACCTCCACT ACTGGTTTGT GGAGTCCCAG AAGGATCCCG AGAACAGCCC TGTGGTGCTT
TGGCTCAATG GGGGTCCCGG CTGCAGCTCA CTAGATGGGC TCCTCACAGA GCATGGCCCC
TTCCTGGTCC AGCCAGATGG TGTCACCCTG GAGTACAACC CCTATTCTTG GAATCTGATT
GCCAATGTGT TATACCTGGA GTCCCCAGCT GGGGTGGGCT TCTCCTACTC CGATGACAAG
TTTTATGCAA CTAATGACAC TGAGGTCGCC CAGAGCAATT TTGAGGCCCT TCAAGATTTC
TTCCGCCTCT TTCCGGAGTA CAAGAACAAC AAACTTTTCC TGACCGGGGA GAGCTATGCT
GGCATCTACA TCCCCACCCT GGCCGTGCTG GTCATGCAGG ATCCCAGCAT GAACCTTCAG
GGGCTGGCTG TGGGCAATGG ACTCTCCTCC TATGAGCAGA ATGACAACTC CCTGGTCTAC
TTTGCCTACT ACCATGGCCT TCTGGGGAAC AGGCTTTGGT CTTCTCTCCA GACCCACTGC
TGCTCTCAAA ACAAGTGTAA CTTCTATGAC AACAAAGACC TGGAATGCGT GACCAATCTT
CAGGAAGTGG CCCGCATCGT GGGCAACTCT GGCCTCAACA TCTACAATCT CTATGCCCCG
TGTGCTGGAG GGGTGCCCAG CCATTTTAGG TATGAGAAGG ACACTGTTGT GGTCCAGGAT
TTGGGCAACA TCTTCACTCG CCTGCCACTC AAGCGGATGT GGCATCAGGC ACTGCTGCGC
TCAGGGGATA AAGTGCGCAT GGACCCCCCC TGCACCAACA CAACAGCTGC TTCCACCTAC
CTCAACAACC CGTACGTGCG GAAGGCCCTC AACATCCCGG AGCAGCTGCC ACAATGGGAC
ATGTGCAACT TTCTGGTAAA CTTACAGTAC CGCCGTCTCT ACCGAAGCAT GAACTCCCAG
TATCTGAAGC TGCTTAGCTC ACAGAAATAC CAGATCCTAT TATATAATGG AGATGTAGAC
ATGGCCTGCA ATTTCATGGG GGATGAGTGG TTTGTGGATT CCCTCAACCA GAAGATGGAG
GTGCAGCGCC GGCCCTGGTT AGTGAAGTAC GGGGACAGCG GGGAGCAGAT TGCCGGCTTC
GTGAAGGAGT TCTCCCACAT CGCCTTTCTC ACGATCAAGG GCGCCGGCCA CATGGTTCCC
ACCGACAAGC CCCTCGCTGC CTTCACCATG TTCTCCCGCT TCCTGAACAA GCAGCCATAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001121167.1
CDS475..1917
Misc Feature(1)430..432(+)
Misc Feature(2)589..1902(+)
Exon (1)1..474
Gene:CTSA
Gene Synonym:
Exon (2)475..668
Gene:CTSA
Gene Synonym:
Exon (3)669..780
Gene:CTSA
Gene Synonym:
Exon (4)781..831
Gene:CTSA
Gene Synonym:
Exon (5)832..918
Gene:CTSA
Gene Synonym:
Exon (6)919..1074
Gene:CTSA
Gene Synonym:
Exon (7)1075..1166
Gene:CTSA
Gene Synonym:
Exon (8)1167..1251
Gene:CTSA
Gene Synonym:
Exon (9)1252..1343
Gene:CTSA
Gene Synonym:
Exon (10)1344..1422
Gene:CTSA
Gene Synonym:
Exon (11)1423..1562
Gene:CTSA
Gene Synonym:
Exon (12)1563..1638
Gene:CTSA
Gene Synonym:
Exon (13)1639..1728
Gene:CTSA
Gene Synonym:
Exon (14)1729..1833
Gene:CTSA
Gene Synonym:
Exon (15)1834..2287
Gene:CTSA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001127695
Organism Homo sapiens (human)
Definition Homo sapiens cathepsin A (CTSA), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001127695

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGATCCGAG CCGCGCCGCC GCCGCTGTTC CTGCTGCTGC TGCTGCTGCT GCTGCTAGTG 
TCCTGGGCGT CCCGAGGCGA GGCAGCCCCC GACCAGGACG AGATCCAGCG CCTCCCCGGG
CTGGCCAAGC AGCCGTCTTT CCGCCAGTAC TCCGGCTACC TCAAAGGCTC CGGCTCCAAG
CACCTCCACT ACTGGTTTGT GGAGTCCCAG AAGGATCCCG AGAACAGCCC TGTGGTGCTT
TGGCTCAATG GGGGTCCCGG CTGCAGCTCA CTAGATGGGC TCCTCACAGA GCATGGCCCC
TTCCTGGTCC AGCCAGATGG TGTCACCCTG GAGTACAACC CCTATTCTTG GAATCTGATT
GCCAATGTGT TATACCTGGA GTCCCCAGCT GGGGTGGGCT TCTCCTACTC CGATGACAAG
TTTTATGCAA CTAATGACAC TGAGGTCGCC CAGAGCAATT TTGAGGCCCT TCAAGATTTC
TTCCGCCTCT TTCCGGAGTA CAAGAACAAC AAACTTTTCC TGACCGGGGA GAGCTATGCT
GGCATCTACA TCCCCACCCT GGCCGTGCTG GTCATGCAGG ATCCCAGCAT GAACCTTCAG
GGGCTGGCTG TGGGCAATGG ACTCTCCTCC TATGAGCAGA ATGACAACTC CCTGGTCTAC
TTTGCCTACT ACCATGGCCT TCTGGGGAAC AGGCTTTGGT CTTCTCTCCA GACCCACTGC
TGCTCTCAAA ACAAGTGTAA CTTCTATGAC AACAAAGACC TGGAATGCGT GACCAATCTT
CAGGAAGTGG CCCGCATCGT GGGCAACTCT GGCCTCAACA TCTACAATCT CTATGCCCCG
TGTGCTGGAG GGGTGCCCAG CCATTTTAGG TATGAGAAGG ACACTGTTGT GGTCCAGGAT
TTGGGCAACA TCTTCACTCG CCTGCCACTC AAGCGGATGT GGCATCAGGC ACTGCTGCGC
TCAGGGGATA AAGTGCGCAT GGACCCCCCC TGCACCAACA CAACAGCTGC TTCCACCTAC
CTCAACAACC CGTACGTGCG GAAGGCCCTC AACATCCCGG AGCAGCTGCC ACAATGGGAC
ATGTGCAACT TTCTGGTAAA CTTACAGTAC CGCCGTCTCT ACCGAAGCAT GAACTCCCAG
TATCTGAAGC TGCTTAGCTC ACAGAAATAC CAGATCCTAT TATATAATGG AGATGTAGAC
ATGGCCTGCA ATTTCATGGG GGATGAGTGG TTTGTGGATT CCCTCAACCA GAAGATGGAG
GTGCAGCGCC GGCCCTGGTT AGTGAAGTAC GGGGACAGCG GGGAGCAGAT TGCCGGCTTC
GTGAAGGAGT TCTCCCACAT CGCCTTTCTC ACGATCAAGG GCGCCGGCCA CATGGTTCCC
ACCGACAAGC CCCTCGCTGC CTTCACCATG TTCTCCCGCT TCCTGAACAA GCAGCCATAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu04054
Clone ID Related Accession (Same CDS sequence) NM_001167594
Accession Version NM_001167594.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1446bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product lysosomal protective protein isoform c precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA029446.1, AK304536.1, AK172808.1 and BC000597.2. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The resulting protein is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304536.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACTTCCA GTCCCCGGGC GCCTCCTGGA GAGCAAGGAC GCGGGGGAGC AGAGATGATC 
CGAGCCGCGC CGCCGCCGCT GTTCCTGCTG CTGCTGCTGC TGCTGCTGCT AGTGTCCTGG
GCGTCCCGAG GCGAGGCAGC CCCCGACCAG GACGAGATCC AGCGCCTCCC CGGGCTGGCC
AAGCAGCCGT CTTTCCGCCA GTACTCCGGC TACCTCAAAG GCTCCGGCTC CAAGCACCTC
CACTACTGGT TTGTGGAGTC CCAGAAGGAT CCCGAGAACA GCCCTGTGGT GCTTTGGCTC
AATGGGGGTC CCGGCTGCAG CTCACTAGAT GGGCTCCTCA CAGAGCATGG CCCCTTCCTG
ATTGCCAATG TGTTATACCT GGAGTCCCCA GCTGGGGTGG GCTTCTCCTA CTCCGATGAC
AAGTTTTATG CAACTAATGA CACTGAGGTC GCCCAGAGCA ATTTTGAGGC CCTTCAAGAT
TTCTTCCGCC TCTTTCCGGA GTACAAGAAC AACAAACTTT TCCTGACCGG GGAGAGCTAT
GCTGGCATCT ACATCCCCAC CCTGGCCGTG CTGGTCATGC AGGATCCCAG CATGAACCTT
CAGGGGCTGG CTGTGGGCAA TGGACTCTCC TCCTATGAGC AGAATGACAA CTCCCTGGTC
TACTTTGCCT ACTACCATGG CCTTCTGGGG AACAGGCTTT GGTCTTCTCT CCAGACCCAC
TGCTGCTCTC AAAACAAGTG TAACTTCTAT GACAACAAAG ACCTGGAATG CGTGACCAAT
CTTCAGGAAG TGGCCCGCAT CGTGGGCAAC TCTGGCCTCA ACATCTACAA TCTCTATGCC
CCGTGTGCTG GAGGGGTGCC CAGCCATTTT AGGTATGAGA AGGACACTGT TGTGGTCCAG
GATTTGGGCA ACATCTTCAC TCGCCTGCCA CTCAAGCGGA TGTGGCATCA GGCACTGCTG
CGCTCAGGGG ATAAAGTGCG CATGGACCCC CCCTGCACCA ACACAACAGC TGCTTCCACC
TACCTCAACA ACCCGTACGT GCGGAAGGCC CTCAACATCC CGGAGCAGCT GCCACAATGG
GACATGTGCA ACTTTCTGGT AAACTTACAG TACCGCCGTC TCTACCGAAG CATGAACTCC
CAGTATCTGA AGCTGCTTAG CTCACAGAAA TACCAGATCC TATTATATAA TGGAGATGTA
GACATGGCCT GCAATTTCAT GGGGGATGAG TGGTTTGTGG ATTCCCTCAA CCAGAAGATG
GAGGTGCAGC GCCGGCCCTG GTTAGTGAAG TACGGGGACA GCGGGGAGCA GATTGCCGGC
TTCGTGAAGG AGTTCTCCCA CATCGCCTTT CTCACGATCA AGGGCGCCGG CCACATGGTT
CCCACCGACA AGCCCCTCGC TGCCTTCACC ATGTTCTCCC GCTTCCTGAA CAAGCAGCCA
TACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001161066.1
CDS375..1820
Misc Feature(1)363..365(+)
Misc Feature(2)543..1805(+)
Exon (1)1..428
Gene:CTSA
Gene Synonym:
Exon (2)429..622
Gene:CTSA
Gene Synonym:
Exon (3)623..734
Gene:CTSA
Gene Synonym:
Exon (4)735..821
Gene:CTSA
Gene Synonym:
Exon (5)822..977
Gene:CTSA
Gene Synonym:
Exon (6)978..1069
Gene:CTSA
Gene Synonym:
Exon (7)1070..1154
Gene:CTSA
Gene Synonym:
Exon (8)1155..1246
Gene:CTSA
Gene Synonym:
Exon (9)1247..1325
Gene:CTSA
Gene Synonym:
Exon (10)1326..1465
Gene:CTSA
Gene Synonym:
Exon (11)1466..1541
Gene:CTSA
Gene Synonym:
Exon (12)1542..1631
Gene:CTSA
Gene Synonym:
Exon (13)1632..1736
Gene:CTSA
Gene Synonym:
Exon (14)1737..2190
Gene:CTSA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001167594
Organism Homo sapiens (human)
Definition Homo sapiens cathepsin A (CTSA), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001167594

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGACTTCCA GTCCCCGGGC GCCTCCTGGA GAGCAAGGAC GCGGGGGAGC AGAGATGATC 
CGAGCCGCGC CGCCGCCGCT GTTCCTGCTG CTGCTGCTGC TGCTGCTGCT AGTGTCCTGG
GCGTCCCGAG GCGAGGCAGC CCCCGACCAG GACGAGATCC AGCGCCTCCC CGGGCTGGCC
AAGCAGCCGT CTTTCCGCCA GTACTCCGGC TACCTCAAAG GCTCCGGCTC CAAGCACCTC
CACTACTGGT TTGTGGAGTC CCAGAAGGAT CCCGAGAACA GCCCTGTGGT GCTTTGGCTC
AATGGGGGTC CCGGCTGCAG CTCACTAGAT GGGCTCCTCA CAGAGCATGG CCCCTTCCTG
ATTGCCAATG TGTTATACCT GGAGTCCCCA GCTGGGGTGG GCTTCTCCTA CTCCGATGAC
AAGTTTTATG CAACTAATGA CACTGAGGTC GCCCAGAGCA ATTTTGAGGC CCTTCAAGAT
TTCTTCCGCC TCTTTCCGGA GTACAAGAAC AACAAACTTT TCCTGACCGG GGAGAGCTAT
GCTGGCATCT ACATCCCCAC CCTGGCCGTG CTGGTCATGC AGGATCCCAG CATGAACCTT
CAGGGGCTGG CTGTGGGCAA TGGACTCTCC TCCTATGAGC AGAATGACAA CTCCCTGGTC
TACTTTGCCT ACTACCATGG CCTTCTGGGG AACAGGCTTT GGTCTTCTCT CCAGACCCAC
TGCTGCTCTC AAAACAAGTG TAACTTCTAT GACAACAAAG ACCTGGAATG CGTGACCAAT
CTTCAGGAAG TGGCCCGCAT CGTGGGCAAC TCTGGCCTCA ACATCTACAA TCTCTATGCC
CCGTGTGCTG GAGGGGTGCC CAGCCATTTT AGGTATGAGA AGGACACTGT TGTGGTCCAG
GATTTGGGCA ACATCTTCAC TCGCCTGCCA CTCAAGCGGA TGTGGCATCA GGCACTGCTG
CGCTCAGGGG ATAAAGTGCG CATGGACCCC CCCTGCACCA ACACAACAGC TGCTTCCACC
TACCTCAACA ACCCGTACGT GCGGAAGGCC CTCAACATCC CGGAGCAGCT GCCACAATGG
GACATGTGCA ACTTTCTGGT AAACTTACAG TACCGCCGTC TCTACCGAAG CATGAACTCC
CAGTATCTGA AGCTGCTTAG CTCACAGAAA TACCAGATCC TATTATATAA TGGAGATGTA
GACATGGCCT GCAATTTCAT GGGGGATGAG TGGTTTGTGG ATTCCCTCAA CCAGAAGATG
GAGGTGCAGC GCCGGCCCTG GTTAGTGAAG TACGGGGACA GCGGGGAGCA GATTGCCGGC
TTCGTGAAGG AGTTCTCCCA CATCGCCTTT CTCACGATCA AGGGCGCCGG CCACATGGTT
CCCACCGACA AGCCCCTCGC TGCCTTCACC ATGTTCTCCC GCTTCCTGAA CAAGCAGCCA
TACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Inhibition of CatA: an emerging strategy for the treatment of heart failure
Future Med Chem 5 (4), 399-409 (2013)
Ruf S, Buning C, Schreuder H, Linz W, Hubschle T, Linz D, Ruetten H, Wirth K and Sadowski T.


book

Galactosialidosis: review and analysis of CTSA gene mutations
Orphanet J Rare Dis 8, 114 (2013)
Caciotti A, Catarzi S, Tonin R, Lugli L, Perez CR, Michelakakis H, Mavridou I, Donati MA, Guerrini R, d'Azzo A and Morrone A.


book

Role of cathepsin A and cathepsin C in the regulation of glycosidase activity
Folia Histochem. Cytobiol. 50 (1), 20-24 (2012)
Minarowska A, Minarowski L, Karwowska A, Milewska AJ and Gacko M.


book

Cathepsin A is expressed in primary human antigen-presenting cells
Immunol. Lett. 128 (2), 143-147 (2010)
Reich M, Spindler KD, Burret M, Kalbacher H, Boehm BO and Burster T.


book

Heterodimerization of the sialidase NEU1 with the chaperone protective protein/cathepsin A prevents its premature oligomerization
J. Biol. Chem. 284 (41), 28430-28441 (2009)
Bonten EJ, Campos Y, Zaitsev V, Nourse A, Waddell B, Lewis W, Taylor G and d'Azzo A.


book

Human lysosomal protective protein. Glycosylation, intracellular transport, and association with beta-galactosidase in the endoplasmic reticulum
J. Biol. Chem. 267 (25), 17949-17956 (1992)
Morreau H, Galjart NJ, Willemsen R, Gillemans N, Zhou XY and d'Azzo A.


book

Ring chromosome 20 and possible assignment of the structural gene encoding human carboxypeptidase-L to the distal segment of the long arm of chromosome 20
Am. J. Med. Genet. 43 (3), 576-579 (1992)
Halal F, Chitayat D, Parikh H, Rosenblatt B, Tranchemontagne J, Vekemans M and Potier M.


book

A mutation in a mild form of galactosialidosis impairs dimerization of the protective protein and renders it unstable
EMBO J. 10 (13), 4041-4048 (1991)
Zhou XY, Galjart NJ, Willemsen R, Gillemans N, Galjaard H and d'Azzo A.


book

Human lysosomal protective protein has cathepsin A-like activity distinct from its protective function
J. Biol. Chem. 266 (22), 14754-14762 (1991)
Galjart NJ, Morreau H, Willemsen R, Gillemans N, Bonten EJ and d'Azzo A.


book

A peptidase in human platelets that deamidates tachykinins. Probable identity with the lysosomal 'protective protein'
J. Biol. Chem. 265 (19), 11265-11272 (1990)
Jackman HL, Tan FL, Tamei H, Beurling-Harbury C, Li XY, Skidgel RA and Erdos EG.


 
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