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MKS1 Meckel syndrome, type 1 [Homo sapiens (human)]

Gene Symbol MKS1
Entrez Gene ID 54903
Full Name Meckel syndrome, type 1
Synonyms BBS13, MES, MKS, POC12
General protein information
Preferred Names
Meckel syndrome type 1 protein
Names
Meckel syndrome type 1 protein
FABB proteome-like protein
POC12 centriolar protein homolog
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q22

Summary The protein encoded by this gene localizes to the basal body and is required for formation of the primary cilium in ciliated epithelial cells. Mutations in this gene result in Meckel syndrome type 1 and in Bardet-Biedl syndrome type 13. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]. lac of sum
Disorder MIM:

609883

Disorder Html: Meckel syndrome, type 1, 249000 (3); Bardet-Biedl syndrome 13,
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu68758 XM_011524957 PREDICTED: Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu68759 XM_011524958 PREDICTED: Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu50503 XM_005257483 PREDICTED: Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu68760 XM_011524959 PREDICTED: Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu68761 XM_011524960 PREDICTED: Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu50506 XM_005257485 PREDICTED: Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu50507 XM_006721965 PREDICTED: Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu50508 XM_005257486 PREDICTED: Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu26205 NM_017777 Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu26353 NM_001165927 Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu68758D
Sequence Information ORF Nucleotide Sequence (Length: 1695bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product Meckel syndrome type 1 protein isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA TCTTTGGAGG TGGACACCGC CCAGAGGAAG ACGAAGAGGA GGAGATTGTG
ATTGGGTGGC AGGAGAAGCT CTTTAGCCAG TTTGAAGTAG ATCTGTACCA AAATGAAACA
GCCTGTCAGA GTCCTTTGGA TTATCAGTAC CGTCAGGAGA TCCTGAAGCT GGAGAATTCG
GGTGGCAAGA AAAACCGACG AATCTTTACC TACACTGACT CTGATAGATA CACCAATTTG
GAGGAGCACT GTCAGAGAAT GACCACTGCA GCCAGCGAGG TGCCTTCATT CTTGGTCGAG
CGAATGGCAA ATGTCAGGCG TCGCCGGCAG GACAGGCGAG GGATGGAGGG CGGCATCCTC
AAGTCACGCA TCGTCACCTG GGAGCCCTCA GAAGAGTTTG TCAGGAACAA CCACGTCATT
AACACCCCTC TTCAGACAAT GCACATCATG GCAGACCTGG GGCCCTATAA AAAGCTTGGC
TATAAGAAGT ATGAACATGT CCTGTGTACT CTGAAGGTGG ATAGCAATGG TGTGATCACA
GTAAAGCCTG ACTTCACGGG CCTCAAAGGA CCCTACAGGA TTGAGACGGA GGGGGAGAAG
CAGGAGCTGT GGAAATATAC GATCGACAAT GTTTCCCCCC ACGCACAGCC GGAGGAGGAG
GAGCGGGAAC GGCGAGTGTT CAAGGATCTT TATGGCCGGC ACAAGGAGTA TCTCAGCAGC
CTCGTAGGCA CCGACTTTGA GATGACTGTC CCAGGTGCCC TCCGGCTCTT TGTAAATGGA
GAGGTCGTTT CAGCCCAAGG CTATGAGTAT GACAATCTCT ACGTCCACTT CTTTGTAGAA
TTGCCAACTG CTCACTGGTC AAGCCCAGCA TTCCAGCAGC TCTCAGGAGT AACACAGACC
TGCACCACCA AGTCCCTGGC AATGGACAAG GTGGCTCACT TCTCCTACCC ATTCACGTTT
GAAGCCTTCT TCCTCCATGA GGATGAATCT TCTGATGCAC TCCCGGAGTG GCCTGTGCTC
TACTGTGAGG TCCTCTCGCT GGACTTCTGG CAGAGGTACC GTGTGGAAGG CTATGGGGCT
GTGGTGCTGC CTGCCACTCC AGGCTCACAC ACCCTGACAG TCTCCACGTG GAGACCTGTG
GAGCTTGGCA CGGTGGCTGA GCTGAGGAGG TTTTTCATTG GCGGTTCTCT GGAACTGGAG
GACCTCTCCT ATGTACGGAT ACCAGGATCC TTCAAGGGCC TTCATGGAAT CGAGCTCCCT
TCAGAAAAGG ATGCGGAGTG TGTTGGACCG TCTGGAAGGG TTCAGCCAGC AGAGTTCCAT
TCACAATGTG CTAGAGGCCT TCCGTCGAGC CCGGCGCCGC ATGCAGGAGG CCCGGGAAAG
CCTCCCGCAG GACCTAGTGA GCCCCTCTGG AACCCTGGTC TCCTAGCTCA CAGCAGCCCT
GGCCCACAGT GCAAGAGGAC AAGATGGGGG ATATCTGAGG CCAGTGCTCT CCTGCCTCTT
CGTCTTTCTG ATTAG
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011523259.1
CDS49..1743
Misc Feature(1)991..1473(+)
Translation
Position Chain Variation Link
4 4 a, c dbSNP:777068143
8 8 a, c dbSNP:761615481
9 9 a, g, t dbSNP:760700958
14 14 g, t dbSNP:775593405
15 15 c, t dbSNP:772383483
29 29 c, g, t dbSNP:115507363
31 31 c, g dbSNP:116514023
34 34 c, g dbSNP:541448010
48 48 c, g dbSNP:573940561
53 53 c, t dbSNP:377040370
60 60 c, t dbSNP:372471169
63 63 c, t dbSNP:780539112
71 71 c, t dbSNP:754729482
75 75 c, t dbSNP:369488349
78 78 c, g, t dbSNP:576239597
79 79 a, g dbSNP:762103206
80 80 c, g dbSNP:375223375
100 100 a, c dbSNP:557896607
102 102 -, cccgg dbSNP:730880323
103 103 -, ccggg dbSNP:386834051
118 118 c, t dbSNP:538371478
131 131 c, t dbSNP:201957874
140 140 a, g dbSNP:767646864
149 149 c, t dbSNP:759465861
158 158 a, g, t dbSNP:587779734
162 162 c, t dbSNP:375861391
163 163 c, t dbSNP:11653070
166 166 c, t dbSNP:199832333
183 183 c, g dbSNP:766174298
184 184 a, g dbSNP:183617764
194 194 a, g dbSNP:748133097
196 196 a, g dbSNP:776363123
199 199 c, t dbSNP:368169919
203 203 c, t dbSNP:746450191
212 212 c, t dbSNP:779521346
213 213 c, t dbSNP:375999060
232 232 -, actgcca dbSNP:386834046
232 232 a, g dbSNP:758090787
242 242 g, t dbSNP:748412372
247 247 a, g dbSNP:201362733
248 248 a, g dbSNP:755502660
253 253 c, t dbSNP:756716716
256 256 c, t dbSNP:200340896
257 257 a, g dbSNP:780293222
261 261 a, t dbSNP:557752726
265 265 a, g dbSNP:750702019
267 267 a, g dbSNP:778759498
270 270 c, g dbSNP:142813109
271 271 a, g dbSNP:753620277
280 280 a, g dbSNP:763949111
282 282 c, g, t dbSNP:775351056
290 290 g, t dbSNP:786204222
291 291 c, t dbSNP:771577659
292 292 a, g dbSNP:759008278
293 293 g, t dbSNP:774076532
306 306 a, g dbSNP:62636631
326 326 c, t dbSNP:367668056
333 333 a, g dbSNP:747325433
336 336 c, t dbSNP:187870415
337 337 a, c dbSNP:76838735
339 339 a, g dbSNP:772742739
343 343 a, g dbSNP:76192155
365 365 c, t dbSNP:201574278
370 370 c, t dbSNP:778921113
379 379 c, t dbSNP:201476674
380 380 a, g dbSNP:200331553
402 402 g, t dbSNP:777934536
407 407 c, t dbSNP:755927059
408 408 a, g dbSNP:370781583
416 416 a, g dbSNP:767269565
417 417 a, g, t dbSNP:751455442
422 422 a, c dbSNP:765809969
424 424 -, c dbSNP:775043799
424 424 c, t dbSNP:762482919
425 425 a, g dbSNP:202112856
428 428 a, g dbSNP:765026950
438 438 c, g dbSNP:761521921
439 439 c, t dbSNP:75699185
440 440 a, g dbSNP:776068083
442 442 a, g dbSNP:374506610
449 449 -, ct dbSNP:386834047
456 456 a, g dbSNP:746378310
458 458 a, g dbSNP:747461404
468 468 a, g dbSNP:770948877
471 471 g, t dbSNP:749341221
474 474 a, g dbSNP:386834048
478 478 g, t dbSNP:200332126
481 481 c, t dbSNP:386834049
484 484 a, g dbSNP:185130897
485 485 g, t dbSNP:758424317
504 504 c, t dbSNP:750376903
509 509 c, t dbSNP:778584579
517 517 c, t dbSNP:576624384
518 518 c, t dbSNP:753579811
522 522 a, g dbSNP:763755799
525 525 c, t dbSNP:199684837
526 526 a, g dbSNP:200970148
527 527 a, g dbSNP:766648988
529 529 c, t dbSNP:386834050
530 530 g, t dbSNP:373471917
533 533 c, t dbSNP:370013310
535 535 a, g dbSNP:761830955
538 538 a, g dbSNP:776666925
547 547 c, t dbSNP:370568445
548 548 a, g dbSNP:200149256
550 550 c, t dbSNP:779953982
551 551 a, g dbSNP:771709346
553 553 c, t dbSNP:201845154
555 555 g, t dbSNP:778956664
565 565 c, t dbSNP:756853299
566 566 a, g dbSNP:369388608
579 579 c, t dbSNP:780657802
580 580 a, g dbSNP:754601373
581 581 g, t dbSNP:527350238
590 590 -, a dbSNP:769896408
595 595 c, t dbSNP:559966703
596 596 a, g, t dbSNP:145764409
598 598 a, g dbSNP:764242484
601 601 a, g dbSNP:200185068
605 605 c, t dbSNP:775805558
610 610 a, g dbSNP:373755286
611 611 a, g dbSNP:571769092
637 637 a, g dbSNP:759802849
642 642 c, t dbSNP:200628275
643 643 a, g dbSNP:140677541
675 675 a, c, g dbSNP:772982926
690 690 g, t dbSNP:769368392
691 691 a, c dbSNP:747659750
694 694 g, t dbSNP:780721543
695 695 a, g dbSNP:754689401
696 696 c, t dbSNP:746647549
705 705 g, t dbSNP:769659359
710 710 a, g dbSNP:201758855
724 724 c, t dbSNP:776172667
729 729 c, t dbSNP:200531517
730 730 c, t dbSNP:370632053
750 750 c, t dbSNP:777474025
757 757 g, t dbSNP:746666115
761 761 c, t dbSNP:557691735
770 770 g, t dbSNP:376785849
776 776 a, c dbSNP:771310059
785 785 c, t dbSNP:749668169
786 786 a, g, t dbSNP:373491923
789 789 c, g dbSNP:753234130
793 793 -, aaag dbSNP:781397982
814 814 a, g dbSNP:745531071
815 815 c, t dbSNP:778058178
816 816 a, g dbSNP:770140009
819 819 a, g dbSNP:748670448
820 820 a, c, g, t dbSNP:201237547
823 823 a, g dbSNP:780276924
823 823 -, g dbSNP:751818062
848 848 c, t dbSNP:561482424
852 852 c, t dbSNP:750597104
853 853 a, g dbSNP:764943455
857 857 a, g dbSNP:757032985
858 858 c, t dbSNP:753798549
861 861 c, t dbSNP:764069453
862 862 g, t dbSNP:567771507
866 866 a, c dbSNP:368751106
868 868 a, c, g, t dbSNP:201771125
870 870 a, c, t dbSNP:201961765
878 878 c, t dbSNP:770088105
879 879 a, g dbSNP:375557871
880 880 a, g dbSNP:777103638
891 891 -, gag dbSNP:780100856
892 892 c, t dbSNP:189255737
893 893 a, g dbSNP:747504986
898 898 c, t dbSNP:780007842
902 902 a, g dbSNP:184444815
906 906 a, g dbSNP:746105434
910 910 a, c dbSNP:779149583
912 912 a, g dbSNP:757400825
914 914 a, g dbSNP:151023718
916 916 c, t dbSNP:755916212
925 925 c, t dbSNP:777558323
926 926 a, g dbSNP:755840522
927 927 g, t dbSNP:769489826
931 931 a, g dbSNP:201845569
942 942 c, t dbSNP:781136688
949 949 c, t dbSNP:78774579
951 951 c, t dbSNP:754839039
952 952 a, g dbSNP:751146857
953 953 c, t dbSNP:765793774
960 960 c, t dbSNP:142805406
961 961 a, g dbSNP:750025608
966 966 c, t dbSNP:764581050
972 972 a, g dbSNP:761070122
973 973 a, g dbSNP:761528251
975 975 c, t dbSNP:373269341
991 991 c, t dbSNP:767763740
992 992 c, g dbSNP:369483945
1000 1000 g, t dbSNP:774654581
1001 1001 a, t dbSNP:771030303
1010 1010 a, g dbSNP:762973500
1011 1011 g, t dbSNP:773255938
1014 1014 c, t dbSNP:770067106
1015 1015 a, g dbSNP:386834053
1028 1028 a, g dbSNP:776673480
1034 1034 a, g dbSNP:768515749
1050 1050 c, t dbSNP:552668070
1051 1051 a, g dbSNP:775391594
1059 1059 -, ctt dbSNP:765577615
1064 1064 a, t dbSNP:772205041
1066 1066 a, g dbSNP:745789469
1071 1071 a, g dbSNP:201998680
1085 1085 a, c, g dbSNP:567026924
1088 1088 c, g dbSNP:760971749
1093 1093 c, t dbSNP:538164532
1095 1095 a, g dbSNP:775485640
1103 1103 a, g dbSNP:375559691
1105 1105 c, g, t dbSNP:386834041
1115 1115 a, g dbSNP:771939064
1120 1120 a, g dbSNP:745809472
1123 1123 c, t dbSNP:786205508
1127 1127 c, t dbSNP:774391311
1128 1128 -, c dbSNP:762377424
1135 1135 a, g dbSNP:770497568
1143 1143 c, t dbSNP:748800331
1146 1146 a, g dbSNP:777308590
1147 1147 c, g dbSNP:769389013
1150 1150 a, g dbSNP:750926734
1159 1159 a, g dbSNP:762800635
1169 1169 -, tct dbSNP:587777804
1172 1172 -, cct dbSNP:754279998
1178 1178 c, g dbSNP:773157492
1180 1180 a, t dbSNP:769312467
1184 1184 c, t dbSNP:200350173
1185 1185 a, g dbSNP:369141016
1191 1191 a, g dbSNP:77365082
1194 1194 a, c dbSNP:746657618
1195 1195 g, t dbSNP:774595103
1201 1201 c, t dbSNP:771262017
1223 1223 a, t dbSNP:373004412
1232 1232 c, t dbSNP:763534380
1233 1233 a, g, t dbSNP:775169308
1253 1253 c, g, t dbSNP:144764478
1263 1263 c, t dbSNP:202040080
1265 1265 c, g dbSNP:773684291
1266 1266 a, g dbSNP:770493819
1270 1270 a, g dbSNP:372554696
1274 1274 -, t dbSNP:758918957
1279 1279 c, t dbSNP:781423785
1284 1284 a, g dbSNP:755065589
1286 1286 a, g dbSNP:774267957
1288 1288 c, t dbSNP:201036775
1289 1289 a, c, g dbSNP:200679238
1290 1290 c, t dbSNP:201128765
1301 1301 a, g dbSNP:765002709
1317 1317 a, c, g, t dbSNP:749099410
1324 1324 a, g dbSNP:763627565
1325 1325 c, t dbSNP:760184188
1326 1326 c, t dbSNP:772818256
1330 1330 c, g dbSNP:774840755
1336 1336 c, t dbSNP:141741656
1341 1341 c, t dbSNP:761247194
1344 1344 a, c, g dbSNP:555977738
1351 1351 c, t dbSNP:759662552
1352 1352 c, t dbSNP:774384333
1355 1355 c, t dbSNP:755841031
1356 1356 a, g dbSNP:749503564
1375 1375 a, g dbSNP:773075697
1379 1379 c, t dbSNP:367625961
1380 1380 a, c, g dbSNP:781038098
1382 1382 g, t dbSNP:754930606
1383 1383 a, g dbSNP:569499621
1389 1389 a, g dbSNP:746581223
1397 1397 a, g dbSNP:779697924
1406 1406 c, t dbSNP:200865108
1410 1410 a, c, t dbSNP:764549118
1411 1411 a, g dbSNP:538819956
1412 1412 -, gtg dbSNP:373382151
1413 1413 g, t dbSNP:753085014
1420 1420 c, g dbSNP:199927741
1436 1436 c, t dbSNP:760032525
1439 1439 a, g dbSNP:730882120
1440 1440 c, t dbSNP:774475723
1444 1444 c, t dbSNP:766392300
1445 1445 a, g dbSNP:201619500
1446 1446 g, t dbSNP:773269657
1471 1471 -, c dbSNP:780161503
1472 1472 a, g dbSNP:144635826
1479 1479 c, g, t dbSNP:758838271
1480 1480 a, g dbSNP:368535131
1499 1499 a, g dbSNP:778890248
1502 1502 c, t dbSNP:757310695
1503 1503 a, g dbSNP:373843986
1505 1505 a, g dbSNP:9906421
1508 1508 a, g dbSNP:370130538
1517 1517 c, t dbSNP:775558298
1518 1518 a, g, t dbSNP:200658872
1527 1527 a, g dbSNP:767285168
1545 1545 c, g dbSNP:759400183
1554 1554 a, g dbSNP:774309110
1564 1564 g, t dbSNP:767250536
1566 1566 a, c, g dbSNP:751294519
1571 1571 c, t dbSNP:745946583
1572 1572 a, g, t dbSNP:779093781
1574 1574 a, c dbSNP:772719574
1575 1575 a, g dbSNP:199910690
1576 1576 a, c dbSNP:761483576
1579 1579 c, g dbSNP:35493987
1580 1580 c, t dbSNP:768171144
1581 1581 a, g dbSNP:746283445
1583 1583 c, t dbSNP:35464956
1584 1584 a, g dbSNP:771624307
1586 1586 c, t dbSNP:34631184
1587 1587 a, g, t dbSNP:557678962
1594 1594 a, g dbSNP:267604965
1601 1601 c, t dbSNP:748406509
1603 1603 a, g dbSNP:781530535
1608 1608 a, g dbSNP:755128244
1609 1609 a, c dbSNP:545364645
1613 1613 c, t dbSNP:751425104
1614 1614 c, t dbSNP:771585740
1615 1615 a, g dbSNP:758334200
1627 1627 c, g dbSNP:34092089
1635 1635 c, g dbSNP:764747820
1642 1642 c, t dbSNP:553314050
1645 1645 a, c, g dbSNP:11548967
1648 1648 c, t dbSNP:376925280
1657 1657 c, t dbSNP:774792843
1664 1664 g, t dbSNP:771356715
1665 1665 c, t dbSNP:763587314
1681 1681 a, g dbSNP:566239288
1692 1692 -, a dbSNP:34304018
1696 1696 a, g dbSNP:773809071
1697 1697 a, g dbSNP:369146407
1703 1703 a, c dbSNP:371962780
1712 1712 c, g dbSNP:35384582
1729 1729 c, t dbSNP:35184412
1750 1750 c, t dbSNP:568504276
1751 1751 a, g dbSNP:550885201
1788 1788 c, t dbSNP:34224064
1789 1789 a, g dbSNP:565533857
1806 1806 a, g dbSNP:780840500
1809 1809 c, t dbSNP:754562608
1867 1867 c, t dbSNP:751103496
1976 1976 g, t dbSNP:185405908
1997 1997 c, g dbSNP:759501344
2002 2002 c, g dbSNP:781131831
2005 2005 a, g dbSNP:528253185
2005 2005 -, g dbSNP:35298266
2020 2020 a, g dbSNP:768932890
2025 2025 a, g dbSNP:370149098
2029 2029 c, g, t dbSNP:200682912
2068 2068 c, t dbSNP:2302313
2069 2069 a, g dbSNP:750194631
2076 2076 a, g dbSNP:778861333
2123 2123 c, t dbSNP:386465709
2126 2126 c, g dbSNP:17174788
2160 2160 a, g dbSNP:545361117
2176 2176 -, t dbSNP:777353151
2189 2189 a, g dbSNP:760382239
2200 2200 a, g dbSNP:752254231
2209 2209 c, t dbSNP:1045619
2248 2248 g, t dbSNP:371037402
2257 2257 g, t dbSNP:578200260

Target ORF information:

RefSeq Version XM_011524957
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011524957

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA TCTTTGGAGG TGGACACCGC CCAGAGGAAG ACGAAGAGGA GGAGATTGTG
ATTGGGTGGC AGGAGAAGCT CTTTAGCCAG TTTGAAGTAG ATCTGTACCA AAATGAAACA
GCCTGTCAGA GTCCTTTGGA TTATCAGTAC CGTCAGGAGA TCCTGAAGCT GGAGAATTCG
GGTGGCAAGA AAAACCGACG AATCTTTACC TACACTGACT CTGATAGATA CACCAATTTG
GAGGAGCACT GTCAGAGAAT GACCACTGCA GCCAGCGAGG TGCCTTCATT CTTGGTCGAG
CGAATGGCAA ATGTCAGGCG TCGCCGGCAG GACAGGCGAG GGATGGAGGG CGGCATCCTC
AAGTCACGCA TCGTCACCTG GGAGCCCTCA GAAGAGTTTG TCAGGAACAA CCACGTCATT
AACACCCCTC TTCAGACAAT GCACATCATG GCAGACCTGG GGCCCTATAA AAAGCTTGGC
TATAAGAAGT ATGAACATGT CCTGTGTACT CTGAAGGTGG ATAGCAATGG TGTGATCACA
GTAAAGCCTG ACTTCACGGG CCTCAAAGGA CCCTACAGGA TTGAGACGGA GGGGGAGAAG
CAGGAGCTGT GGAAATATAC GATCGACAAT GTTTCCCCCC ACGCACAGCC GGAGGAGGAG
GAGCGGGAAC GGCGAGTGTT CAAGGATCTT TATGGCCGGC ACAAGGAGTA TCTCAGCAGC
CTCGTAGGCA CCGACTTTGA GATGACTGTC CCAGGTGCCC TCCGGCTCTT TGTAAATGGA
GAGGTCGTTT CAGCCCAAGG CTATGAGTAT GACAATCTCT ACGTCCACTT CTTTGTAGAA
TTGCCAACTG CTCACTGGTC AAGCCCAGCA TTCCAGCAGC TCTCAGGAGT AACACAGACC
TGCACCACCA AGTCCCTGGC AATGGACAAG GTGGCTCACT TCTCCTACCC ATTCACGTTT
GAAGCCTTCT TCCTCCATGA GGATGAATCT TCTGATGCAC TCCCGGAGTG GCCTGTGCTC
TACTGTGAGG TCCTCTCGCT GGACTTCTGG CAGAGGTACC GTGTGGAAGG CTATGGGGCT
GTGGTGCTGC CTGCCACTCC AGGCTCACAC ACCCTGACAG TCTCCACGTG GAGACCTGTG
GAGCTTGGCA CGGTGGCTGA GCTGAGGAGG TTTTTCATTG GCGGTTCTCT GGAACTGGAG
GACCTCTCCT ATGTACGGAT ACCAGGATCC TTCAAGGGCC TTCATGGAAT CGAGCTCCCT
TCAGAAAAGG ATGCGGAGTG TGTTGGACCG TCTGGAAGGG TTCAGCCAGC AGAGTTCCAT
TCACAATGTG CTAGAGGCCT TCCGTCGAGC CCGGCGCCGC ATGCAGGAGG CCCGGGAAAG
CCTCCCGCAG GACCTAGTGA GCCCCTCTGG AACCCTGGTC TCCTAGCTCA CAGCAGCCCT
GGCCCACAGT GCAAGAGGAC AAGATGGGGG ATATCTGAGG CCAGTGCTCT CCTGCCTCTT
CGTCTTTCTG ATTAG

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu68759D
Sequence Information ORF Nucleotide Sequence (Length: 1689bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product Meckel syndrome type 1 protein isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA TCTTTGGAGG TGGACACCGC CCAGAGGAAG ACGAAGAGGA GGAGATTGTG
ATTGGGTGGC AGGAGAAGCT CTTTAGCCAG TTTGAAGTAG ATCTGTACCA AAATGAAACA
GCCTGTCAGA GTCCTTTGGA TTATCAGTAC CGTCAGGAGA TCCTGAAGCT GGAGAATTCG
GGTGGCAAGA AAAACCGACG AATCTTTACC TACACTGACT CTGATAGATA CACCAATTTG
GAGGAGCACT GTCAGAGAAT GACCACTGCA GCCAGCGAGG TGCCTTCATT CTTGGTCGAG
CGAATGGCAA ATGTCAGGCG TCGCCGGCAG GACAGGCGAG GGATGGAGGG CGGCATCCTC
AAGTCACGCA TCGTCACCTG GGAGCCCTCA GAAGAGTTTG TCAGGAACAA CCACGTCATT
AACACCCCTC TTCAGACAAT GCACATCATG GCAGACCTGG GGCCCTATAA AAAGCTTGGC
TATAAGAAGT ATGAACATGT CCTGTGTACT CTGAAGGTGG ATAGCAATGG TGTGATCACA
GTAAAGCCTG ACTTCACGGG CCTCAAAGGA CCCTACAGGA TTGAGACGGA GGGGGAGAAG
CAGGAGCTGT GGAAATATAC GATCGACAAT GTTTCCCCCC ACGCACAGCC GGAGGAGGAG
GAGCGGGAAC GGCGAGTGTT CAAGGATCTT TATGGCCGGC ACAAGGAGTA TCTCAGCAGC
CTCGTAGGCA CCGACTTTGA GATGACTGTC CCAGGTGCCC TCCGGCTCTT TGTAAATGGA
GAGGTCGTTT CAGCCCAAGG CTATGAGTAT GACAATCTCT ACGTCCACTT CTTTGTAGAA
TTGCCAACTG CTCACTGGTC AAGCCCAGCA TTCCAGCAGC TCTCAGGAGT AACACAGACC
TGCACCACCA AGTCCCTGGC AATGGACAAG GTGGCTCACT TCTCCTACCC ATTCACGTTT
GAAGCCTTCT TCCTCCATGA GGATGAATCT TCTGATGCAC TCCCGGAGTG GCCTGTGCTC
TACTGTGAGG TCCTCTCGCT GGACTTCTGG CAGAGGTACC GTGTGGAAGG CTATGGGGCT
GTGGTGCTGC CTGCCACTCC AGGCTCACAC ACCCTGACAG TCTCCACGTG GAGACCTGTG
GAGCTTGGCA CGGTGGCTGA GCTGAGGAGG TTTTTCATTG GCGGTTCTCT GGAACTGGAG
GACCTCTCCT ATGTACGGAT ACCAGGATCC TTCAAGGGGG AACGCCTGAG CCGCTTTGGA
CTCCGCACAG AGACCACAGG CACTGTCACC TTCCGCTTGC ACTGTCTGCA GCAGTCCAGG
GCCTTCATGG AATCGAGCTC CCTTCAGAAA AGGATGCGGA GTGTGTTGGA CCGTCTGGAA
GGGTTCAGCC AGCAGAGTTC CATTCACAAT GTGCTAGAGG CCTTCCGTCG AGCCCGGCGC
CGCATGCAGG AGGCCCGGGA AAGCCTCCCG CAGGACCTAG TGAGCCCCTC TGGAACCCTG
GTCTCCTAG
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011523260.1
CDS49..1737
Misc Feature(1)991..1539(+)
Translation
Position Chain Variation Link
4 4 a, c dbSNP:777068143
8 8 a, c dbSNP:761615481
9 9 a, g, t dbSNP:760700958
14 14 g, t dbSNP:775593405
15 15 c, t dbSNP:772383483
29 29 c, g, t dbSNP:115507363
31 31 c, g dbSNP:116514023
34 34 c, g dbSNP:541448010
48 48 c, g dbSNP:573940561
53 53 c, t dbSNP:377040370
60 60 c, t dbSNP:372471169
63 63 c, t dbSNP:780539112
71 71 c, t dbSNP:754729482
75 75 c, t dbSNP:369488349
78 78 c, g, t dbSNP:576239597
79 79 a, g dbSNP:762103206
80 80 c, g dbSNP:375223375
100 100 a, c dbSNP:557896607
102 102 -, cccgg dbSNP:730880323
103 103 -, ccggg dbSNP:386834051
118 118 c, t dbSNP:538371478
131 131 c, t dbSNP:201957874
140 140 a, g dbSNP:767646864
149 149 c, t dbSNP:759465861
158 158 a, g, t dbSNP:587779734
162 162 c, t dbSNP:375861391
163 163 c, t dbSNP:11653070
166 166 c, t dbSNP:199832333
183 183 c, g dbSNP:766174298
184 184 a, g dbSNP:183617764
194 194 a, g dbSNP:748133097
196 196 a, g dbSNP:776363123
199 199 c, t dbSNP:368169919
203 203 c, t dbSNP:746450191
212 212 c, t dbSNP:779521346
213 213 c, t dbSNP:375999060
232 232 -, actgcca dbSNP:386834046
232 232 a, g dbSNP:758090787
242 242 g, t dbSNP:748412372
247 247 a, g dbSNP:201362733
248 248 a, g dbSNP:755502660
253 253 c, t dbSNP:756716716
256 256 c, t dbSNP:200340896
257 257 a, g dbSNP:780293222
261 261 a, t dbSNP:557752726
265 265 a, g dbSNP:750702019
267 267 a, g dbSNP:778759498
270 270 c, g dbSNP:142813109
271 271 a, g dbSNP:753620277
280 280 a, g dbSNP:763949111
282 282 c, g, t dbSNP:775351056
290 290 g, t dbSNP:786204222
291 291 c, t dbSNP:771577659
292 292 a, g dbSNP:759008278
293 293 g, t dbSNP:774076532
306 306 a, g dbSNP:62636631
326 326 c, t dbSNP:367668056
333 333 a, g dbSNP:747325433
336 336 c, t dbSNP:187870415
337 337 a, c dbSNP:76838735
339 339 a, g dbSNP:772742739
343 343 a, g dbSNP:76192155
365 365 c, t dbSNP:201574278
370 370 c, t dbSNP:778921113
379 379 c, t dbSNP:201476674
380 380 a, g dbSNP:200331553
402 402 g, t dbSNP:777934536
407 407 c, t dbSNP:755927059
408 408 a, g dbSNP:370781583
416 416 a, g dbSNP:767269565
417 417 a, g, t dbSNP:751455442
422 422 a, c dbSNP:765809969
424 424 -, c dbSNP:775043799
424 424 c, t dbSNP:762482919
425 425 a, g dbSNP:202112856
428 428 a, g dbSNP:765026950
438 438 c, g dbSNP:761521921
439 439 c, t dbSNP:75699185
440 440 a, g dbSNP:776068083
442 442 a, g dbSNP:374506610
449 449 -, ct dbSNP:386834047
456 456 a, g dbSNP:746378310
458 458 a, g dbSNP:747461404
468 468 a, g dbSNP:770948877
471 471 g, t dbSNP:749341221
474 474 a, g dbSNP:386834048
478 478 g, t dbSNP:200332126
481 481 c, t dbSNP:386834049
484 484 a, g dbSNP:185130897
485 485 g, t dbSNP:758424317
504 504 c, t dbSNP:750376903
509 509 c, t dbSNP:778584579
517 517 c, t dbSNP:576624384
518 518 c, t dbSNP:753579811
522 522 a, g dbSNP:763755799
525 525 c, t dbSNP:199684837
526 526 a, g dbSNP:200970148
527 527 a, g dbSNP:766648988
529 529 c, t dbSNP:386834050
530 530 g, t dbSNP:373471917
533 533 c, t dbSNP:370013310
535 535 a, g dbSNP:761830955
538 538 a, g dbSNP:776666925
547 547 c, t dbSNP:370568445
548 548 a, g dbSNP:200149256
550 550 c, t dbSNP:779953982
551 551 a, g dbSNP:771709346
553 553 c, t dbSNP:201845154
555 555 g, t dbSNP:778956664
565 565 c, t dbSNP:756853299
566 566 a, g dbSNP:369388608
579 579 c, t dbSNP:780657802
580 580 a, g dbSNP:754601373
581 581 g, t dbSNP:527350238
590 590 -, a dbSNP:769896408
595 595 c, t dbSNP:559966703
596 596 a, g, t dbSNP:145764409
598 598 a, g dbSNP:764242484
601 601 a, g dbSNP:200185068
605 605 c, t dbSNP:775805558
610 610 a, g dbSNP:373755286
611 611 a, g dbSNP:571769092
637 637 a, g dbSNP:759802849
642 642 c, t dbSNP:200628275
643 643 a, g dbSNP:140677541
675 675 a, c, g dbSNP:772982926
690 690 g, t dbSNP:769368392
691 691 a, c dbSNP:747659750
694 694 g, t dbSNP:780721543
695 695 a, g dbSNP:754689401
696 696 c, t dbSNP:746647549
705 705 g, t dbSNP:769659359
710 710 a, g dbSNP:201758855
724 724 c, t dbSNP:776172667
729 729 c, t dbSNP:200531517
730 730 c, t dbSNP:370632053
750 750 c, t dbSNP:777474025
757 757 g, t dbSNP:746666115
761 761 c, t dbSNP:557691735
770 770 g, t dbSNP:376785849
776 776 a, c dbSNP:771310059
785 785 c, t dbSNP:749668169
786 786 a, g, t dbSNP:373491923
789 789 c, g dbSNP:753234130
793 793 -, aaag dbSNP:781397982
814 814 a, g dbSNP:745531071
815 815 c, t dbSNP:778058178
816 816 a, g dbSNP:770140009
819 819 a, g dbSNP:748670448
820 820 a, c, g, t dbSNP:201237547
823 823 a, g dbSNP:780276924
823 823 -, g dbSNP:751818062
848 848 c, t dbSNP:561482424
852 852 c, t dbSNP:750597104
853 853 a, g dbSNP:764943455
857 857 a, g dbSNP:757032985
858 858 c, t dbSNP:753798549
861 861 c, t dbSNP:764069453
862 862 g, t dbSNP:567771507
866 866 a, c dbSNP:368751106
868 868 a, c, g, t dbSNP:201771125
870 870 a, c, t dbSNP:201961765
878 878 c, t dbSNP:770088105
879 879 a, g dbSNP:375557871
880 880 a, g dbSNP:777103638
891 891 -, gag dbSNP:780100856
892 892 c, t dbSNP:189255737
893 893 a, g dbSNP:747504986
898 898 c, t dbSNP:780007842
902 902 a, g dbSNP:184444815
906 906 a, g dbSNP:746105434
910 910 a, c dbSNP:779149583
912 912 a, g dbSNP:757400825
914 914 a, g dbSNP:151023718
916 916 c, t dbSNP:755916212
925 925 c, t dbSNP:777558323
926 926 a, g dbSNP:755840522
927 927 g, t dbSNP:769489826
931 931 a, g dbSNP:201845569
942 942 c, t dbSNP:781136688
949 949 c, t dbSNP:78774579
951 951 c, t dbSNP:754839039
952 952 a, g dbSNP:751146857
953 953 c, t dbSNP:765793774
960 960 c, t dbSNP:142805406
961 961 a, g dbSNP:750025608
966 966 c, t dbSNP:764581050
972 972 a, g dbSNP:761070122
973 973 a, g dbSNP:761528251
975 975 c, t dbSNP:373269341
991 991 c, t dbSNP:767763740
992 992 c, g dbSNP:369483945
1000 1000 g, t dbSNP:774654581
1001 1001 a, t dbSNP:771030303
1010 1010 a, g dbSNP:762973500
1011 1011 g, t dbSNP:773255938
1014 1014 c, t dbSNP:770067106
1015 1015 a, g dbSNP:386834053
1028 1028 a, g dbSNP:776673480
1034 1034 a, g dbSNP:768515749
1050 1050 c, t dbSNP:552668070
1051 1051 a, g dbSNP:775391594
1059 1059 -, ctt dbSNP:765577615
1064 1064 a, t dbSNP:772205041
1066 1066 a, g dbSNP:745789469
1071 1071 a, g dbSNP:201998680
1085 1085 a, c, g dbSNP:567026924
1088 1088 c, g dbSNP:760971749
1093 1093 c, t dbSNP:538164532
1095 1095 a, g dbSNP:775485640
1103 1103 a, g dbSNP:375559691
1105 1105 c, g, t dbSNP:386834041
1115 1115 a, g dbSNP:771939064
1120 1120 a, g dbSNP:745809472
1123 1123 c, t dbSNP:786205508
1127 1127 c, t dbSNP:774391311
1128 1128 -, c dbSNP:762377424
1135 1135 a, g dbSNP:770497568
1143 1143 c, t dbSNP:748800331
1146 1146 a, g dbSNP:777308590
1147 1147 c, g dbSNP:769389013
1150 1150 a, g dbSNP:750926734
1159 1159 a, g dbSNP:762800635
1169 1169 -, tct dbSNP:587777804
1172 1172 -, cct dbSNP:754279998
1178 1178 c, g dbSNP:773157492
1180 1180 a, t dbSNP:769312467
1184 1184 c, t dbSNP:200350173
1185 1185 a, g dbSNP:369141016
1191 1191 a, g dbSNP:77365082
1194 1194 a, c dbSNP:746657618
1195 1195 g, t dbSNP:774595103
1201 1201 c, t dbSNP:771262017
1223 1223 a, t dbSNP:373004412
1232 1232 c, t dbSNP:763534380
1233 1233 a, g, t dbSNP:775169308
1253 1253 c, g, t dbSNP:144764478
1263 1263 c, t dbSNP:202040080
1265 1265 c, g dbSNP:773684291
1266 1266 a, g dbSNP:770493819
1270 1270 a, g dbSNP:372554696
1274 1274 -, t dbSNP:758918957
1279 1279 c, t dbSNP:781423785
1284 1284 a, g dbSNP:755065589
1286 1286 a, g dbSNP:774267957
1288 1288 c, t dbSNP:201036775
1289 1289 a, c, g dbSNP:200679238
1290 1290 c, t dbSNP:201128765
1301 1301 a, g dbSNP:765002709
1317 1317 a, c, g, t dbSNP:749099410
1324 1324 a, g dbSNP:763627565
1325 1325 c, t dbSNP:760184188
1326 1326 c, t dbSNP:772818256
1330 1330 c, g dbSNP:774840755
1336 1336 c, t dbSNP:141741656
1341 1341 c, t dbSNP:761247194
1344 1344 a, c, g dbSNP:555977738
1351 1351 c, t dbSNP:759662552
1352 1352 c, t dbSNP:774384333
1355 1355 c, t dbSNP:755841031
1356 1356 a, g dbSNP:749503564
1375 1375 a, g dbSNP:773075697
1379 1379 c, t dbSNP:367625961
1380 1380 a, c, g dbSNP:781038098
1382 1382 g, t dbSNP:754930606
1383 1383 a, g dbSNP:569499621
1389 1389 a, g dbSNP:746581223
1397 1397 a, g dbSNP:779697924
1406 1406 c, t dbSNP:200865108
1410 1410 a, c, t dbSNP:764549118
1411 1411 a, g dbSNP:538819956
1412 1412 -, gtg dbSNP:373382151
1413 1413 g, t dbSNP:753085014
1420 1420 c, g dbSNP:199927741
1436 1436 c, t dbSNP:760032525
1439 1439 a, g dbSNP:730882120
1440 1440 c, t dbSNP:774475723
1444 1444 c, t dbSNP:766392300
1445 1445 a, g dbSNP:201619500
1446 1446 g, t dbSNP:773269657
1465 1465 g, t dbSNP:566204379
1468 1468 -, g dbSNP:762668200
1468 1468 c, g dbSNP:776303055
1471 1471 c, g, t dbSNP:181513926
1472 1472 a, g, t dbSNP:772114886
1480 1480 c, t dbSNP:529604036
1481 1481 a, g dbSNP:200026560
1487 1487 g, t dbSNP:770548274
1492 1492 c, t dbSNP:769442220
1493 1493 a, g dbSNP:111315726
1494 1494 a, c dbSNP:755297587
1504 1504 a, c dbSNP:369380403
1510 1510 -, ggca dbSNP:386834044
1515 1515 c, t dbSNP:780576848
1521 1521 c, g dbSNP:758937277
1532 1532 a, g dbSNP:750522668
1533 1533 g, t dbSNP:137853105
1534 1534 a, c dbSNP:761944624
1536 1536 a, g dbSNP:376833168
1544 1544 a, c dbSNP:764000969
1547 1547 a, g dbSNP:386834045
1554 1554 -, c dbSNP:780161503
1555 1555 a, g dbSNP:144635826
1562 1562 c, g, t dbSNP:758838271
1563 1563 a, g dbSNP:368535131
1582 1582 a, g dbSNP:778890248
1585 1585 c, t dbSNP:757310695
1586 1586 a, g dbSNP:373843986
1588 1588 a, g dbSNP:9906421
1591 1591 a, g dbSNP:370130538
1600 1600 c, t dbSNP:775558298
1601 1601 a, g, t dbSNP:200658872
1610 1610 a, g dbSNP:767285168
1628 1628 c, g dbSNP:759400183
1637 1637 a, g dbSNP:774309110
1647 1647 g, t dbSNP:767250536
1649 1649 a, c, g dbSNP:751294519
1654 1654 c, t dbSNP:745946583
1655 1655 a, g, t dbSNP:779093781
1657 1657 a, c dbSNP:772719574
1658 1658 a, g dbSNP:199910690
1659 1659 a, c dbSNP:761483576
1662 1662 c, g dbSNP:35493987
1663 1663 c, t dbSNP:768171144
1664 1664 a, g dbSNP:746283445
1666 1666 c, t dbSNP:35464956
1667 1667 a, g dbSNP:771624307
1669 1669 c, t dbSNP:34631184
1670 1670 a, g, t dbSNP:557678962
1677 1677 a, g dbSNP:267604965
1684 1684 c, t dbSNP:748406509
1686 1686 a, g dbSNP:781530535
1691 1691 a, g dbSNP:755128244
1692 1692 a, c dbSNP:545364645
1696 1696 c, t dbSNP:751425104
1697 1697 c, t dbSNP:771585740
1698 1698 a, g dbSNP:758334200
1710 1710 c, g dbSNP:34092089
1718 1718 c, g dbSNP:764747820
1725 1725 c, t dbSNP:553314050
1728 1728 a, c, g dbSNP:11548967
1731 1731 c, t dbSNP:376925280
1740 1740 c, t dbSNP:774792843
1747 1747 g, t dbSNP:771356715
1748 1748 c, t dbSNP:763587314
1764 1764 a, g dbSNP:566239288
1775 1775 -, a dbSNP:34304018
1779 1779 a, g dbSNP:773809071
1780 1780 a, g dbSNP:369146407
1786 1786 a, c dbSNP:371962780
1795 1795 c, g dbSNP:35384582
1812 1812 c, t dbSNP:35184412
1833 1833 c, t dbSNP:568504276
1834 1834 a, g dbSNP:550885201
1871 1871 c, t dbSNP:34224064
1872 1872 a, g dbSNP:565533857
1889 1889 a, g dbSNP:780840500
1892 1892 c, t dbSNP:754562608
1950 1950 c, t dbSNP:751103496
2059 2059 g, t dbSNP:185405908
2080 2080 c, g dbSNP:759501344
2085 2085 c, g dbSNP:781131831
2088 2088 a, g dbSNP:528253185
2088 2088 -, g dbSNP:35298266
2103 2103 a, g dbSNP:768932890
2108 2108 a, g dbSNP:370149098
2112 2112 c, g, t dbSNP:200682912
2151 2151 c, t dbSNP:2302313
2152 2152 a, g dbSNP:750194631
2159 2159 a, g dbSNP:778861333
2206 2206 c, t dbSNP:386465709
2209 2209 c, g dbSNP:17174788
2243 2243 a, g dbSNP:545361117
2259 2259 -, t dbSNP:777353151
2272 2272 a, g dbSNP:760382239
2283 2283 a, g dbSNP:752254231
2292 2292 c, t dbSNP:1045619
2331 2331 g, t dbSNP:371037402
2340 2340 g, t dbSNP:578200260

Target ORF information:

RefSeq Version XM_011524958
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011524958

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA TCTTTGGAGG TGGACACCGC CCAGAGGAAG ACGAAGAGGA GGAGATTGTG
ATTGGGTGGC AGGAGAAGCT CTTTAGCCAG TTTGAAGTAG ATCTGTACCA AAATGAAACA
GCCTGTCAGA GTCCTTTGGA TTATCAGTAC CGTCAGGAGA TCCTGAAGCT GGAGAATTCG
GGTGGCAAGA AAAACCGACG AATCTTTACC TACACTGACT CTGATAGATA CACCAATTTG
GAGGAGCACT GTCAGAGAAT GACCACTGCA GCCAGCGAGG TGCCTTCATT CTTGGTCGAG
CGAATGGCAA ATGTCAGGCG TCGCCGGCAG GACAGGCGAG GGATGGAGGG CGGCATCCTC
AAGTCACGCA TCGTCACCTG GGAGCCCTCA GAAGAGTTTG TCAGGAACAA CCACGTCATT
AACACCCCTC TTCAGACAAT GCACATCATG GCAGACCTGG GGCCCTATAA AAAGCTTGGC
TATAAGAAGT ATGAACATGT CCTGTGTACT CTGAAGGTGG ATAGCAATGG TGTGATCACA
GTAAAGCCTG ACTTCACGGG CCTCAAAGGA CCCTACAGGA TTGAGACGGA GGGGGAGAAG
CAGGAGCTGT GGAAATATAC GATCGACAAT GTTTCCCCCC ACGCACAGCC GGAGGAGGAG
GAGCGGGAAC GGCGAGTGTT CAAGGATCTT TATGGCCGGC ACAAGGAGTA TCTCAGCAGC
CTCGTAGGCA CCGACTTTGA GATGACTGTC CCAGGTGCCC TCCGGCTCTT TGTAAATGGA
GAGGTCGTTT CAGCCCAAGG CTATGAGTAT GACAATCTCT ACGTCCACTT CTTTGTAGAA
TTGCCAACTG CTCACTGGTC AAGCCCAGCA TTCCAGCAGC TCTCAGGAGT AACACAGACC
TGCACCACCA AGTCCCTGGC AATGGACAAG GTGGCTCACT TCTCCTACCC ATTCACGTTT
GAAGCCTTCT TCCTCCATGA GGATGAATCT TCTGATGCAC TCCCGGAGTG GCCTGTGCTC
TACTGTGAGG TCCTCTCGCT GGACTTCTGG CAGAGGTACC GTGTGGAAGG CTATGGGGCT
GTGGTGCTGC CTGCCACTCC AGGCTCACAC ACCCTGACAG TCTCCACGTG GAGACCTGTG
GAGCTTGGCA CGGTGGCTGA GCTGAGGAGG TTTTTCATTG GCGGTTCTCT GGAACTGGAG
GACCTCTCCT ATGTACGGAT ACCAGGATCC TTCAAGGGGG AACGCCTGAG CCGCTTTGGA
CTCCGCACAG AGACCACAGG CACTGTCACC TTCCGCTTGC ACTGTCTGCA GCAGTCCAGG
GCCTTCATGG AATCGAGCTC CCTTCAGAAA AGGATGCGGA GTGTGTTGGA CCGTCTGGAA
GGGTTCAGCC AGCAGAGTTC CATTCACAAT GTGCTAGAGG CCTTCCGTCG AGCCCGGCGC
CGCATGCAGG AGGCCCGGGA AAGCCTCCCG CAGGACCTAG TGAGCCCCTC TGGAACCCTG
GTCTCCTAG

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu50503D
Sequence Information ORF Nucleotide Sequence (Length: 1686bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product Meckel syndrome type 1 protein isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578831107. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA GTGGACACCG CCCAGAGGAA GACGAAGAGG AGGAGATTGT GATTGGGTGG
CAGGAGAAGC TCTTTAGCCA GTTTGAAGTA GATCTGTACC AAAATGAAAC AGCCTGTCAG
AGTCCTTTGG ATTATCAGTA CCGTCAGGAG ATCCTGAAGC TGGAGAATTC GGGTGGCAAG
AAAAACCGAC GAATCTTTAC CTACACTGAC TCTGATAGAT ACACCAATTT GGAGGAGCAC
TGTCAGAGAA TGACCACTGC AGCCAGCGAG GTGCCTTCAT TCTTGGTCGA GCGAATGGCA
AATGTCAGGC GTCGCCGGCA GGACAGGCGA GGGATGGAGG GCGGCATCCT CAAGTCACGC
ATCGTCACCT GGGAGCCCTC AGAAGAGTTT GTCAGGAACA ACCACGTCAT TAACACCCCT
CTTCAGACAA TGCACATCAT GGCAGACCTG GGGCCCTATA AAAAGCTTGG CTATAAGAAG
TATGAACATG TCCTGTGTAC TCTGAAGGTG GATAGCAATG GTGTGATCAC AGTAAAGCCT
GACTTCACGG GCCTCAAAGG ACCCTACAGG ATTGAGACGG AGGGGGAGAA GCAGGAGCTG
TGGAAATATA CGATCGACAA TGTTTCCCCC CACGCACAGC CGGAGGAGGA GGAGCGGGAA
CGGCGAGTGT TCAAGGATCT TTATGGCCGG CACAAGGAGT ATCTCAGCAG CCTCGTAGGC
ACCGACTTTG AGATGACTGT CCCAGGTGCC CTCCGGCTCT TTGTAAATGG AGAGGTCGTT
TCAGCCCAAG GCTATGAGTA TGACAATCTC TACGTCCACT TCTTTGTAGA ATTGCCAACT
GCTCACTGGT CAAGCCCAGC ATTCCAGCAG CTCTCAGGAG TAACACAGAC CTGCACCACC
AAGTCCCTGG CAATGGACAA GGTGGCTCAC TTCTCCTACC CATTCACGTT TGAAGCCTTC
TTCCTCCATG AGGATGAATC TTCTGATGCA CTCCCGGAGT GGCCTGTGCT CTACTGTGAG
GTCCTCTCGC TGGACTTCTG GCAGAGGTAC CGTGTGGAAG GCTATGGGGC TGTGGTGCTG
CCTGCCACTC CAGGCTCACA CACCCTGACA GTCTCCACGT GGAGACCTGT GGAGCTTGGC
ACGGTGGCTG AGCTGAGGAG GTTTTTCATT GGCGGTTCTC TGGAACTGGA GGACCTCTCC
TATGTACGGA TACCAGGATC CTTCAAGGGC CTTCATGGAA TCGAGCTCCC TTCAGAAAAG
GATGCGGAGT GTGTTGGACC GTCTGGAAGG GTTCAGCCAG CAGAGTTCCA TTCACAATGT
GCTAGAGGCC TTCCGTCGAG CCCGGCGCCG CATGCAGGAG GCCCGGGAAA GCCTCCCGCA
GGACCTAGTG AGCCCCTCTG GAACCCTGGT CTCCTAGCTC ACAGCAGCCC TGGCCCACAG
TGCAAGAGGA CAAGATGGGG GATATCTGAG GCCAGTGCTC TCCTGCCTCT TCGTCTTTCT
GATTAG
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005257540.1
CDS62..1747
Misc Feature(1)995..1477(+)
Translation
Position Chain Variation Link
17 17 a, c dbSNP:777068143
21 21 a, c dbSNP:761615481
22 22 a, g, t dbSNP:760700958
27 27 g, t dbSNP:775593405
28 28 c, t dbSNP:772383483
42 42 c, g, t dbSNP:115507363
44 44 c, g dbSNP:116514023
47 47 c, g dbSNP:541448010
61 61 c, g dbSNP:573940561
66 66 c, t dbSNP:377040370
73 73 c, t dbSNP:372471169
76 76 c, t dbSNP:780539112
84 84 c, t dbSNP:754729482
88 88 c, t dbSNP:369488349
91 91 c, g, t dbSNP:576239597
92 92 a, g dbSNP:762103206
93 93 c, g dbSNP:375223375
113 113 a, c dbSNP:557896607
115 115 -, cccgg dbSNP:730880323
116 116 -, ccggg dbSNP:386834051
131 131 c, t dbSNP:538371478
144 144 c, t dbSNP:201957874
153 153 a, g dbSNP:767646864
162 162 c, t dbSNP:759465861
171 171 a, g, t dbSNP:587779734
175 175 c, t dbSNP:375861391
176 176 c, t dbSNP:11653070
179 179 c, t dbSNP:199832333
196 196 c, g dbSNP:766174298
197 197 a, g dbSNP:183617764
207 207 a, g dbSNP:748133097
209 209 a, g dbSNP:776363123
212 212 c, t dbSNP:368169919
216 216 c, t dbSNP:746450191
225 225 c, t dbSNP:779521346
226 226 c, t dbSNP:375999060
245 245 -, actgcca dbSNP:386834046
245 245 a, g dbSNP:758090787
252 252 a, g dbSNP:755502660
257 257 c, t dbSNP:756716716
260 260 c, t dbSNP:200340896
261 261 a, g dbSNP:780293222
265 265 a, t dbSNP:557752726
269 269 a, g dbSNP:750702019
271 271 a, g dbSNP:778759498
274 274 c, g dbSNP:142813109
275 275 a, g dbSNP:753620277
284 284 a, g dbSNP:763949111
286 286 c, g, t dbSNP:775351056
294 294 g, t dbSNP:786204222
295 295 c, t dbSNP:771577659
296 296 a, g dbSNP:759008278
297 297 g, t dbSNP:774076532
310 310 a, g dbSNP:62636631
330 330 c, t dbSNP:367668056
337 337 a, g dbSNP:747325433
340 340 c, t dbSNP:187870415
341 341 a, c dbSNP:76838735
343 343 a, g dbSNP:772742739
347 347 a, g dbSNP:76192155
369 369 c, t dbSNP:201574278
374 374 c, t dbSNP:778921113
383 383 c, t dbSNP:201476674
384 384 a, g dbSNP:200331553
406 406 g, t dbSNP:777934536
411 411 c, t dbSNP:755927059
412 412 a, g dbSNP:370781583
420 420 a, g dbSNP:767269565
421 421 a, g, t dbSNP:751455442
426 426 a, c dbSNP:765809969
428 428 -, c dbSNP:775043799
428 428 c, t dbSNP:762482919
429 429 a, g dbSNP:202112856
432 432 a, g dbSNP:765026950
442 442 c, g dbSNP:761521921
443 443 c, t dbSNP:75699185
444 444 a, g dbSNP:776068083
446 446 a, g dbSNP:374506610
453 453 -, ct dbSNP:386834047
460 460 a, g dbSNP:746378310
462 462 a, g dbSNP:747461404
472 472 a, g dbSNP:770948877
475 475 g, t dbSNP:749341221
478 478 a, g dbSNP:386834048
482 482 g, t dbSNP:200332126
485 485 c, t dbSNP:386834049
488 488 a, g dbSNP:185130897
489 489 g, t dbSNP:758424317
508 508 c, t dbSNP:750376903
513 513 c, t dbSNP:778584579
521 521 c, t dbSNP:576624384
522 522 c, t dbSNP:753579811
526 526 a, g dbSNP:763755799
529 529 c, t dbSNP:199684837
530 530 a, g dbSNP:200970148
531 531 a, g dbSNP:766648988
533 533 c, t dbSNP:386834050
534 534 g, t dbSNP:373471917
537 537 c, t dbSNP:370013310
539 539 a, g dbSNP:761830955
542 542 a, g dbSNP:776666925
551 551 c, t dbSNP:370568445
552 552 a, g dbSNP:200149256
554 554 c, t dbSNP:779953982
555 555 a, g dbSNP:771709346
557 557 c, t dbSNP:201845154
559 559 g, t dbSNP:778956664
569 569 c, t dbSNP:756853299
570 570 a, g dbSNP:369388608
583 583 c, t dbSNP:780657802
584 584 a, g dbSNP:754601373
585 585 g, t dbSNP:527350238
594 594 -, a dbSNP:769896408
599 599 c, t dbSNP:559966703
600 600 a, g, t dbSNP:145764409
602 602 a, g dbSNP:764242484
605 605 a, g dbSNP:200185068
609 609 c, t dbSNP:775805558
614 614 a, g dbSNP:373755286
615 615 a, g dbSNP:571769092
641 641 a, g dbSNP:759802849
646 646 c, t dbSNP:200628275
647 647 a, g dbSNP:140677541
679 679 a, c, g dbSNP:772982926
694 694 g, t dbSNP:769368392
695 695 a, c dbSNP:747659750
698 698 g, t dbSNP:780721543
699 699 a, g dbSNP:754689401
700 700 c, t dbSNP:746647549
709 709 g, t dbSNP:769659359
714 714 a, g dbSNP:201758855
728 728 c, t dbSNP:776172667
733 733 c, t dbSNP:200531517
734 734 c, t dbSNP:370632053
754 754 c, t dbSNP:777474025
761 761 g, t dbSNP:746666115
765 765 c, t dbSNP:557691735
774 774 g, t dbSNP:376785849
780 780 a, c dbSNP:771310059
789 789 c, t dbSNP:749668169
790 790 a, g, t dbSNP:373491923
793 793 c, g dbSNP:753234130
797 797 -, aaag dbSNP:781397982
818 818 a, g dbSNP:745531071
819 819 c, t dbSNP:778058178
820 820 a, g dbSNP:770140009
823 823 a, g dbSNP:748670448
824 824 a, c, g, t dbSNP:201237547
827 827 a, g dbSNP:780276924
827 827 -, g dbSNP:751818062
852 852 c, t dbSNP:561482424
856 856 c, t dbSNP:750597104
857 857 a, g dbSNP:764943455
861 861 a, g dbSNP:757032985
862 862 c, t dbSNP:753798549
865 865 c, t dbSNP:764069453
866 866 g, t dbSNP:567771507
870 870 a, c dbSNP:368751106
872 872 a, c, g, t dbSNP:201771125
874 874 a, c, t dbSNP:201961765
882 882 c, t dbSNP:770088105
883 883 a, g dbSNP:375557871
884 884 a, g dbSNP:777103638
895 895 -, gag dbSNP:780100856
896 896 c, t dbSNP:189255737
897 897 a, g dbSNP:747504986
902 902 c, t dbSNP:780007842
906 906 a, g dbSNP:184444815
910 910 a, g dbSNP:746105434
914 914 a, c dbSNP:779149583
916 916 a, g dbSNP:757400825
918 918 a, g dbSNP:151023718
920 920 c, t dbSNP:755916212
929 929 c, t dbSNP:777558323
930 930 a, g dbSNP:755840522
931 931 g, t dbSNP:769489826
935 935 a, g dbSNP:201845569
946 946 c, t dbSNP:781136688
953 953 c, t dbSNP:78774579
955 955 c, t dbSNP:754839039
956 956 a, g dbSNP:751146857
957 957 c, t dbSNP:765793774
964 964 c, t dbSNP:142805406
965 965 a, g dbSNP:750025608
970 970 c, t dbSNP:764581050
976 976 a, g dbSNP:761070122
977 977 a, g dbSNP:761528251
979 979 c, t dbSNP:373269341
995 995 c, t dbSNP:767763740
996 996 c, g dbSNP:369483945
1004 1004 g, t dbSNP:774654581
1005 1005 a, t dbSNP:771030303
1014 1014 a, g dbSNP:762973500
1015 1015 g, t dbSNP:773255938
1018 1018 c, t dbSNP:770067106
1019 1019 a, g dbSNP:386834053
1032 1032 a, g dbSNP:776673480
1038 1038 a, g dbSNP:768515749
1054 1054 c, t dbSNP:552668070
1055 1055 a, g dbSNP:775391594
1063 1063 -, ctt dbSNP:765577615
1068 1068 a, t dbSNP:772205041
1070 1070 a, g dbSNP:745789469
1075 1075 a, g dbSNP:201998680
1089 1089 a, c, g dbSNP:567026924
1092 1092 c, g dbSNP:760971749
1097 1097 c, t dbSNP:538164532
1099 1099 a, g dbSNP:775485640
1107 1107 a, g dbSNP:375559691
1109 1109 c, g, t dbSNP:386834041
1119 1119 a, g dbSNP:771939064
1124 1124 a, g dbSNP:745809472
1127 1127 c, t dbSNP:786205508
1131 1131 c, t dbSNP:774391311
1132 1132 -, c dbSNP:762377424
1139 1139 a, g dbSNP:770497568
1147 1147 c, t dbSNP:748800331
1150 1150 a, g dbSNP:777308590
1151 1151 c, g dbSNP:769389013
1154 1154 a, g dbSNP:750926734
1163 1163 a, g dbSNP:762800635
1173 1173 -, tct dbSNP:587777804
1176 1176 -, cct dbSNP:754279998
1182 1182 c, g dbSNP:773157492
1184 1184 a, t dbSNP:769312467
1188 1188 c, t dbSNP:200350173
1189 1189 a, g dbSNP:369141016
1195 1195 a, g dbSNP:77365082
1198 1198 a, c dbSNP:746657618
1199 1199 g, t dbSNP:774595103
1205 1205 c, t dbSNP:771262017
1227 1227 a, t dbSNP:373004412
1236 1236 c, t dbSNP:763534380
1237 1237 a, g, t dbSNP:775169308
1257 1257 c, g, t dbSNP:144764478
1267 1267 c, t dbSNP:202040080
1269 1269 c, g dbSNP:773684291
1270 1270 a, g dbSNP:770493819
1274 1274 a, g dbSNP:372554696
1278 1278 -, t dbSNP:758918957
1283 1283 c, t dbSNP:781423785
1288 1288 a, g dbSNP:755065589
1290 1290 a, g dbSNP:774267957
1292 1292 c, t dbSNP:201036775
1293 1293 a, c, g dbSNP:200679238
1294 1294 c, t dbSNP:201128765
1305 1305 a, g dbSNP:765002709
1321 1321 a, c, g, t dbSNP:749099410
1328 1328 a, g dbSNP:763627565
1329 1329 c, t dbSNP:760184188
1330 1330 c, t dbSNP:772818256
1334 1334 c, g dbSNP:774840755
1340 1340 c, t dbSNP:141741656
1345 1345 c, t dbSNP:761247194
1348 1348 a, c, g dbSNP:555977738
1355 1355 c, t dbSNP:759662552
1356 1356 c, t dbSNP:774384333
1359 1359 c, t dbSNP:755841031
1360 1360 a, g dbSNP:749503564
1379 1379 a, g dbSNP:773075697
1383 1383 c, t dbSNP:367625961
1384 1384 a, c, g dbSNP:781038098
1386 1386 g, t dbSNP:754930606
1387 1387 a, g dbSNP:569499621
1393 1393 a, g dbSNP:746581223
1401 1401 a, g dbSNP:779697924
1410 1410 c, t dbSNP:200865108
1414 1414 a, c, t dbSNP:764549118
1415 1415 a, g dbSNP:538819956
1416 1416 -, gtg dbSNP:373382151
1417 1417 g, t dbSNP:753085014
1424 1424 c, g dbSNP:199927741
1440 1440 c, t dbSNP:760032525
1443 1443 a, g dbSNP:730882120
1444 1444 c, t dbSNP:774475723
1448 1448 c, t dbSNP:766392300
1449 1449 a, g dbSNP:201619500
1450 1450 g, t dbSNP:773269657
1475 1475 -, c dbSNP:780161503
1476 1476 a, g dbSNP:144635826
1483 1483 c, g, t dbSNP:758838271
1484 1484 a, g dbSNP:368535131
1503 1503 a, g dbSNP:778890248
1506 1506 c, t dbSNP:757310695
1507 1507 a, g dbSNP:373843986
1509 1509 a, g dbSNP:9906421
1512 1512 a, g dbSNP:370130538
1521 1521 c, t dbSNP:775558298
1522 1522 a, g, t dbSNP:200658872
1531 1531 a, g dbSNP:767285168
1549 1549 c, g dbSNP:759400183
1558 1558 a, g dbSNP:774309110
1568 1568 g, t dbSNP:767250536
1570 1570 a, c, g dbSNP:751294519
1575 1575 c, t dbSNP:745946583
1576 1576 a, g, t dbSNP:779093781
1578 1578 a, c dbSNP:772719574
1579 1579 a, g dbSNP:199910690
1580 1580 a, c dbSNP:761483576
1583 1583 c, g dbSNP:35493987
1584 1584 c, t dbSNP:768171144
1585 1585 a, g dbSNP:746283445
1587 1587 c, t dbSNP:35464956
1588 1588 a, g dbSNP:771624307
1590 1590 c, t dbSNP:34631184
1591 1591 a, g, t dbSNP:557678962
1598 1598 a, g dbSNP:267604965
1605 1605 c, t dbSNP:748406509
1607 1607 a, g dbSNP:781530535
1612 1612 a, g dbSNP:755128244
1613 1613 a, c dbSNP:545364645
1617 1617 c, t dbSNP:751425104
1618 1618 c, t dbSNP:771585740
1619 1619 a, g dbSNP:758334200
1631 1631 c, g dbSNP:34092089
1639 1639 c, g dbSNP:764747820
1646 1646 c, t dbSNP:553314050
1649 1649 a, c, g dbSNP:11548967
1652 1652 c, t dbSNP:376925280
1661 1661 c, t dbSNP:774792843
1668 1668 g, t dbSNP:771356715
1669 1669 c, t dbSNP:763587314
1685 1685 a, g dbSNP:566239288
1696 1696 -, a dbSNP:34304018
1700 1700 a, g dbSNP:773809071
1701 1701 a, g dbSNP:369146407
1707 1707 a, c dbSNP:371962780
1716 1716 c, g dbSNP:35384582
1733 1733 c, t dbSNP:35184412
1754 1754 c, t dbSNP:568504276
1755 1755 a, g dbSNP:550885201
1792 1792 c, t dbSNP:34224064
1793 1793 a, g dbSNP:565533857
1810 1810 a, g dbSNP:780840500
1813 1813 c, t dbSNP:754562608
1871 1871 c, t dbSNP:751103496
1980 1980 g, t dbSNP:185405908
2001 2001 c, g dbSNP:759501344
2006 2006 c, g dbSNP:781131831
2009 2009 a, g dbSNP:528253185
2009 2009 -, g dbSNP:35298266
2024 2024 a, g dbSNP:768932890
2029 2029 a, g dbSNP:370149098
2033 2033 c, g, t dbSNP:200682912
2072 2072 c, t dbSNP:2302313
2073 2073 a, g dbSNP:750194631
2080 2080 a, g dbSNP:778861333
2127 2127 c, t dbSNP:386465709
2130 2130 c, g dbSNP:17174788
2164 2164 a, g dbSNP:545361117
2180 2180 -, t dbSNP:777353151
2193 2193 a, g dbSNP:760382239
2204 2204 a, g dbSNP:752254231
2213 2213 c, t dbSNP:1045619
2252 2252 g, t dbSNP:371037402
2261 2261 g, t dbSNP:578200260

Target ORF information:

RefSeq Version XM_005257483
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005257483

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA GTGGACACCG CCCAGAGGAA GACGAAGAGG AGGAGATTGT GATTGGGTGG
CAGGAGAAGC TCTTTAGCCA GTTTGAAGTA GATCTGTACC AAAATGAAAC AGCCTGTCAG
AGTCCTTTGG ATTATCAGTA CCGTCAGGAG ATCCTGAAGC TGGAGAATTC GGGTGGCAAG
AAAAACCGAC GAATCTTTAC CTACACTGAC TCTGATAGAT ACACCAATTT GGAGGAGCAC
TGTCAGAGAA TGACCACTGC AGCCAGCGAG GTGCCTTCAT TCTTGGTCGA GCGAATGGCA
AATGTCAGGC GTCGCCGGCA GGACAGGCGA GGGATGGAGG GCGGCATCCT CAAGTCACGC
ATCGTCACCT GGGAGCCCTC AGAAGAGTTT GTCAGGAACA ACCACGTCAT TAACACCCCT
CTTCAGACAA TGCACATCAT GGCAGACCTG GGGCCCTATA AAAAGCTTGG CTATAAGAAG
TATGAACATG TCCTGTGTAC TCTGAAGGTG GATAGCAATG GTGTGATCAC AGTAAAGCCT
GACTTCACGG GCCTCAAAGG ACCCTACAGG ATTGAGACGG AGGGGGAGAA GCAGGAGCTG
TGGAAATATA CGATCGACAA TGTTTCCCCC CACGCACAGC CGGAGGAGGA GGAGCGGGAA
CGGCGAGTGT TCAAGGATCT TTATGGCCGG CACAAGGAGT ATCTCAGCAG CCTCGTAGGC
ACCGACTTTG AGATGACTGT CCCAGGTGCC CTCCGGCTCT TTGTAAATGG AGAGGTCGTT
TCAGCCCAAG GCTATGAGTA TGACAATCTC TACGTCCACT TCTTTGTAGA ATTGCCAACT
GCTCACTGGT CAAGCCCAGC ATTCCAGCAG CTCTCAGGAG TAACACAGAC CTGCACCACC
AAGTCCCTGG CAATGGACAA GGTGGCTCAC TTCTCCTACC CATTCACGTT TGAAGCCTTC
TTCCTCCATG AGGATGAATC TTCTGATGCA CTCCCGGAGT GGCCTGTGCT CTACTGTGAG
GTCCTCTCGC TGGACTTCTG GCAGAGGTAC CGTGTGGAAG GCTATGGGGC TGTGGTGCTG
CCTGCCACTC CAGGCTCACA CACCCTGACA GTCTCCACGT GGAGACCTGT GGAGCTTGGC
ACGGTGGCTG AGCTGAGGAG GTTTTTCATT GGCGGTTCTC TGGAACTGGA GGACCTCTCC
TATGTACGGA TACCAGGATC CTTCAAGGGC CTTCATGGAA TCGAGCTCCC TTCAGAAAAG
GATGCGGAGT GTGTTGGACC GTCTGGAAGG GTTCAGCCAG CAGAGTTCCA TTCACAATGT
GCTAGAGGCC TTCCGTCGAG CCCGGCGCCG CATGCAGGAG GCCCGGGAAA GCCTCCCGCA
GGACCTAGTG AGCCCCTCTG GAACCCTGGT CTCCTAGCTC ACAGCAGCCC TGGCCCACAG
TGCAAGAGGA CAAGATGGGG GATATCTGAG GCCAGTGCTC TCCTGCCTCT TCGTCTTTCT
GATTAG

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu68760D
Sequence Information ORF Nucleotide Sequence (Length: 1380bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product Meckel syndrome type 1 protein isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA TCTTTGGAGG TGGACACCGC CCAGAGGAAG ACGAAGAGGA GGAGATTGTG
ATTGGGTGGC AGGAGAAGCT CTTTAGCCAG TTTGAAGTAG ATCTGTACCA AAATGAAACA
GCCTGTCAGA GTCCTTTGGA TTATCAGTAC CGTCAGGAGA TCCTGAAGCT GGAGAATTCG
GGTGGCAAGA AAAACCGACG AATCTTTACC TACACTGACT CTGATAGATA CACCAATTTG
GAGGAGCACT GTCAGAGAAT GACCACTGCA GCCAGCGAGG TGCCTTCATT CTTGGTCGAG
CGAATGGCAA ATGTCAGGCG TCGCCGGCAG GACAGGCGAG GGATGGAGGG CGGCATCCTC
AAGTCACGCA TCGTCACCTG GGAGCCCTCA GAAGAGTTTG TCAGGAACAA CCACGTCATT
AACACCCCTC TTCAGACAAT GCACATCATG GCAGACCTGG GGCCCTATAA AAAGCTTGGC
TATAAGAAGT ATGAACATGT CCTGTGTACT CTGAAGGTGG ATAGCAATGG TGTGATCACA
GTAAAGCCTG ACTTCACGGG CCTCAAAGGA CCCTACAGGA TTGAGACGGA GGGGGAGAAG
CAGGAGCTGT GGAAATATAC GATCGACAAT GTTTCCCCCC ACGCACAGCC GGAGGAGGAG
GAGCGGGAAC GGCGAGTGTT CAAGGATCTT TATGGCCGGC ACAAGGAGTA TCTCAGCAGC
CTCGTAGGCA CCGACTTTGA GATGACTGTC CCAGGTGCCC TCCGGCTCTT TGTAAATGGA
GAGGTCGTTT CAGCCCAAGG CTATGAGTAT GACAATCTCT ACGTCCACTT CTTTGTAGAA
TTGCCAACTG CTCACTGGTC AAGCCCAGCA TTCCAGCAGC TCTCAGGAGT AACACAGACC
TGCACCACCA AGTCCCTGGC AATGGACAAG GTGGCTCACT TCTCCTACCC ATTCACGTTT
GAAGCCTTCT TCCTCCATGA GGATGAATCT TCTGATGCAC TCCCGGAGTG GCCTGTGCTC
TACTGTGAGG TCCTCTCGCT GGACTTCTGG CAGAGGTACC GTGTGGAAGG CTATGGGGCT
GTGGTGCTGC CTGCCACTCC AGGGCCTTCA TGGAATCGAG CTCCCTTCAG AAAAGGATGC
GGAGTGTGTT GGACCGTCTG GAAGGGTTCA GCCAGCAGAG TTCCATTCAC AATGTGCTAG
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011523261.1
CDS49..1428
Misc Feature(1)991..>1332(+)
Translation
Position Chain Variation Link
4 4 a, c dbSNP:777068143
8 8 a, c dbSNP:761615481
9 9 a, g, t dbSNP:760700958
14 14 g, t dbSNP:775593405
15 15 c, t dbSNP:772383483
29 29 c, g, t dbSNP:115507363
31 31 c, g dbSNP:116514023
34 34 c, g dbSNP:541448010
48 48 c, g dbSNP:573940561
53 53 c, t dbSNP:377040370
60 60 c, t dbSNP:372471169
63 63 c, t dbSNP:780539112
71 71 c, t dbSNP:754729482
75 75 c, t dbSNP:369488349
78 78 c, g, t dbSNP:576239597
79 79 a, g dbSNP:762103206
80 80 c, g dbSNP:375223375
100 100 a, c dbSNP:557896607
102 102 -, cccgg dbSNP:730880323
103 103 -, ccggg dbSNP:386834051
118 118 c, t dbSNP:538371478
131 131 c, t dbSNP:201957874
140 140 a, g dbSNP:767646864
149 149 c, t dbSNP:759465861
158 158 a, g, t dbSNP:587779734
162 162 c, t dbSNP:375861391
163 163 c, t dbSNP:11653070
166 166 c, t dbSNP:199832333
183 183 c, g dbSNP:766174298
184 184 a, g dbSNP:183617764
194 194 a, g dbSNP:748133097
196 196 a, g dbSNP:776363123
199 199 c, t dbSNP:368169919
203 203 c, t dbSNP:746450191
212 212 c, t dbSNP:779521346
213 213 c, t dbSNP:375999060
232 232 -, actgcca dbSNP:386834046
232 232 a, g dbSNP:758090787
242 242 g, t dbSNP:748412372
247 247 a, g dbSNP:201362733
248 248 a, g dbSNP:755502660
253 253 c, t dbSNP:756716716
256 256 c, t dbSNP:200340896
257 257 a, g dbSNP:780293222
261 261 a, t dbSNP:557752726
265 265 a, g dbSNP:750702019
267 267 a, g dbSNP:778759498
270 270 c, g dbSNP:142813109
271 271 a, g dbSNP:753620277
280 280 a, g dbSNP:763949111
282 282 c, g, t dbSNP:775351056
290 290 g, t dbSNP:786204222
291 291 c, t dbSNP:771577659
292 292 a, g dbSNP:759008278
293 293 g, t dbSNP:774076532
306 306 a, g dbSNP:62636631
326 326 c, t dbSNP:367668056
333 333 a, g dbSNP:747325433
336 336 c, t dbSNP:187870415
337 337 a, c dbSNP:76838735
339 339 a, g dbSNP:772742739
343 343 a, g dbSNP:76192155
365 365 c, t dbSNP:201574278
370 370 c, t dbSNP:778921113
379 379 c, t dbSNP:201476674
380 380 a, g dbSNP:200331553
402 402 g, t dbSNP:777934536
407 407 c, t dbSNP:755927059
408 408 a, g dbSNP:370781583
416 416 a, g dbSNP:767269565
417 417 a, g, t dbSNP:751455442
422 422 a, c dbSNP:765809969
424 424 -, c dbSNP:775043799
424 424 c, t dbSNP:762482919
425 425 a, g dbSNP:202112856
428 428 a, g dbSNP:765026950
438 438 c, g dbSNP:761521921
439 439 c, t dbSNP:75699185
440 440 a, g dbSNP:776068083
442 442 a, g dbSNP:374506610
449 449 -, ct dbSNP:386834047
456 456 a, g dbSNP:746378310
458 458 a, g dbSNP:747461404
468 468 a, g dbSNP:770948877
471 471 g, t dbSNP:749341221
474 474 a, g dbSNP:386834048
478 478 g, t dbSNP:200332126
481 481 c, t dbSNP:386834049
484 484 a, g dbSNP:185130897
485 485 g, t dbSNP:758424317
504 504 c, t dbSNP:750376903
509 509 c, t dbSNP:778584579
517 517 c, t dbSNP:576624384
518 518 c, t dbSNP:753579811
522 522 a, g dbSNP:763755799
525 525 c, t dbSNP:199684837
526 526 a, g dbSNP:200970148
527 527 a, g dbSNP:766648988
529 529 c, t dbSNP:386834050
530 530 g, t dbSNP:373471917
533 533 c, t dbSNP:370013310
535 535 a, g dbSNP:761830955
538 538 a, g dbSNP:776666925
547 547 c, t dbSNP:370568445
548 548 a, g dbSNP:200149256
550 550 c, t dbSNP:779953982
551 551 a, g dbSNP:771709346
553 553 c, t dbSNP:201845154
555 555 g, t dbSNP:778956664
565 565 c, t dbSNP:756853299
566 566 a, g dbSNP:369388608
579 579 c, t dbSNP:780657802
580 580 a, g dbSNP:754601373
581 581 g, t dbSNP:527350238
590 590 -, a dbSNP:769896408
595 595 c, t dbSNP:559966703
596 596 a, g, t dbSNP:145764409
598 598 a, g dbSNP:764242484
601 601 a, g dbSNP:200185068
605 605 c, t dbSNP:775805558
610 610 a, g dbSNP:373755286
611 611 a, g dbSNP:571769092
637 637 a, g dbSNP:759802849
642 642 c, t dbSNP:200628275
643 643 a, g dbSNP:140677541
675 675 a, c, g dbSNP:772982926
690 690 g, t dbSNP:769368392
691 691 a, c dbSNP:747659750
694 694 g, t dbSNP:780721543
695 695 a, g dbSNP:754689401
696 696 c, t dbSNP:746647549
705 705 g, t dbSNP:769659359
710 710 a, g dbSNP:201758855
724 724 c, t dbSNP:776172667
729 729 c, t dbSNP:200531517
730 730 c, t dbSNP:370632053
750 750 c, t dbSNP:777474025
757 757 g, t dbSNP:746666115
761 761 c, t dbSNP:557691735
770 770 g, t dbSNP:376785849
776 776 a, c dbSNP:771310059
785 785 c, t dbSNP:749668169
786 786 a, g, t dbSNP:373491923
789 789 c, g dbSNP:753234130
793 793 -, aaag dbSNP:781397982
814 814 a, g dbSNP:745531071
815 815 c, t dbSNP:778058178
816 816 a, g dbSNP:770140009
819 819 a, g dbSNP:748670448
820 820 a, c, g, t dbSNP:201237547
823 823 a, g dbSNP:780276924
823 823 -, g dbSNP:751818062
848 848 c, t dbSNP:561482424
852 852 c, t dbSNP:750597104
853 853 a, g dbSNP:764943455
857 857 a, g dbSNP:757032985
858 858 c, t dbSNP:753798549
861 861 c, t dbSNP:764069453
862 862 g, t dbSNP:567771507
866 866 a, c dbSNP:368751106
868 868 a, c, g, t dbSNP:201771125
870 870 a, c, t dbSNP:201961765
878 878 c, t dbSNP:770088105
879 879 a, g dbSNP:375557871
880 880 a, g dbSNP:777103638
891 891 -, gag dbSNP:780100856
892 892 c, t dbSNP:189255737
893 893 a, g dbSNP:747504986
898 898 c, t dbSNP:780007842
902 902 a, g dbSNP:184444815
906 906 a, g dbSNP:746105434
910 910 a, c dbSNP:779149583
912 912 a, g dbSNP:757400825
914 914 a, g dbSNP:151023718
916 916 c, t dbSNP:755916212
925 925 c, t dbSNP:777558323
926 926 a, g dbSNP:755840522
927 927 g, t dbSNP:769489826
931 931 a, g dbSNP:201845569
942 942 c, t dbSNP:781136688
949 949 c, t dbSNP:78774579
951 951 c, t dbSNP:754839039
952 952 a, g dbSNP:751146857
953 953 c, t dbSNP:765793774
960 960 c, t dbSNP:142805406
961 961 a, g dbSNP:750025608
966 966 c, t dbSNP:764581050
972 972 a, g dbSNP:761070122
973 973 a, g dbSNP:761528251
975 975 c, t dbSNP:373269341
991 991 c, t dbSNP:767763740
992 992 c, g dbSNP:369483945
1000 1000 g, t dbSNP:774654581
1001 1001 a, t dbSNP:771030303
1010 1010 a, g dbSNP:762973500
1011 1011 g, t dbSNP:773255938
1014 1014 c, t dbSNP:770067106
1015 1015 a, g dbSNP:386834053
1028 1028 a, g dbSNP:776673480
1034 1034 a, g dbSNP:768515749
1050 1050 c, t dbSNP:552668070
1051 1051 a, g dbSNP:775391594
1059 1059 -, ctt dbSNP:765577615
1064 1064 a, t dbSNP:772205041
1066 1066 a, g dbSNP:745789469
1071 1071 a, g dbSNP:201998680
1085 1085 a, c, g dbSNP:567026924
1088 1088 c, g dbSNP:760971749
1093 1093 c, t dbSNP:538164532
1095 1095 a, g dbSNP:775485640
1103 1103 a, g dbSNP:375559691
1105 1105 c, g, t dbSNP:386834041
1115 1115 a, g dbSNP:771939064
1120 1120 a, g dbSNP:745809472
1123 1123 c, t dbSNP:786205508
1127 1127 c, t dbSNP:774391311
1128 1128 -, c dbSNP:762377424
1135 1135 a, g dbSNP:770497568
1143 1143 c, t dbSNP:748800331
1146 1146 a, g dbSNP:777308590
1147 1147 c, g dbSNP:769389013
1150 1150 a, g dbSNP:750926734
1159 1159 a, g dbSNP:762800635
1169 1169 -, tct dbSNP:587777804
1172 1172 -, cct dbSNP:754279998
1178 1178 c, g dbSNP:773157492
1180 1180 a, t dbSNP:769312467
1184 1184 c, t dbSNP:200350173
1185 1185 a, g dbSNP:369141016
1191 1191 a, g dbSNP:77365082
1194 1194 a, c dbSNP:746657618
1195 1195 g, t dbSNP:774595103
1201 1201 c, t dbSNP:771262017
1223 1223 a, t dbSNP:373004412
1232 1232 c, t dbSNP:763534380
1233 1233 a, g, t dbSNP:775169308
1253 1253 c, g, t dbSNP:144764478
1263 1263 c, t dbSNP:202040080
1265 1265 c, g dbSNP:773684291
1266 1266 a, g dbSNP:770493819
1270 1270 a, g dbSNP:372554696
1274 1274 -, t dbSNP:758918957
1279 1279 c, t dbSNP:781423785
1284 1284 a, g dbSNP:755065589
1286 1286 a, g dbSNP:774267957
1288 1288 c, t dbSNP:201036775
1289 1289 a, c, g dbSNP:200679238
1290 1290 c, t dbSNP:201128765
1301 1301 a, g dbSNP:765002709
1317 1317 a, c, g, t dbSNP:749099410
1324 1324 a, g dbSNP:763627565
1325 1325 c, t dbSNP:760184188
1326 1326 c, t dbSNP:772818256
1330 1330 c, g dbSNP:774840755
1337 1337 -, c dbSNP:780161503
1338 1338 a, g dbSNP:144635826
1345 1345 c, g, t dbSNP:758838271
1346 1346 a, g dbSNP:368535131
1365 1365 a, g dbSNP:778890248
1368 1368 c, t dbSNP:757310695
1369 1369 a, g dbSNP:373843986
1371 1371 a, g dbSNP:9906421
1374 1374 a, g dbSNP:370130538
1383 1383 c, t dbSNP:775558298
1384 1384 a, g, t dbSNP:200658872
1393 1393 a, g dbSNP:767285168
1411 1411 c, g dbSNP:759400183
1420 1420 a, g dbSNP:774309110
1430 1430 g, t dbSNP:767250536
1432 1432 a, c, g dbSNP:751294519
1437 1437 c, t dbSNP:745946583
1438 1438 a, g, t dbSNP:779093781
1440 1440 a, c dbSNP:772719574
1441 1441 a, g dbSNP:199910690
1442 1442 a, c dbSNP:761483576
1445 1445 c, g dbSNP:35493987
1446 1446 c, t dbSNP:768171144
1447 1447 a, g dbSNP:746283445
1449 1449 c, t dbSNP:35464956
1450 1450 a, g dbSNP:771624307
1452 1452 c, t dbSNP:34631184
1453 1453 a, g, t dbSNP:557678962
1460 1460 a, g dbSNP:267604965
1467 1467 c, t dbSNP:748406509
1469 1469 a, g dbSNP:781530535
1474 1474 a, g dbSNP:755128244
1475 1475 a, c dbSNP:545364645
1479 1479 c, t dbSNP:751425104
1480 1480 c, t dbSNP:771585740
1481 1481 a, g dbSNP:758334200
1493 1493 c, g dbSNP:34092089
1501 1501 c, g dbSNP:764747820
1508 1508 c, t dbSNP:553314050
1511 1511 a, c, g dbSNP:11548967
1514 1514 c, t dbSNP:376925280
1523 1523 c, t dbSNP:774792843
1530 1530 g, t dbSNP:771356715
1531 1531 c, t dbSNP:763587314
1547 1547 a, g dbSNP:566239288
1558 1558 -, a dbSNP:34304018
1562 1562 a, g dbSNP:773809071
1563 1563 a, g dbSNP:369146407
1569 1569 a, c dbSNP:371962780
1578 1578 c, g dbSNP:35384582
1595 1595 c, t dbSNP:35184412
1616 1616 c, t dbSNP:568504276
1617 1617 a, g dbSNP:550885201
1654 1654 c, t dbSNP:34224064
1655 1655 a, g dbSNP:565533857
1672 1672 a, g dbSNP:780840500
1675 1675 c, t dbSNP:754562608
1733 1733 c, t dbSNP:751103496
1842 1842 g, t dbSNP:185405908
1863 1863 c, g dbSNP:759501344
1868 1868 c, g dbSNP:781131831
1871 1871 a, g dbSNP:528253185
1871 1871 -, g dbSNP:35298266
1886 1886 a, g dbSNP:768932890
1891 1891 a, g dbSNP:370149098
1895 1895 c, g, t dbSNP:200682912
1934 1934 c, t dbSNP:2302313
1935 1935 a, g dbSNP:750194631
1942 1942 a, g dbSNP:778861333
1989 1989 c, t dbSNP:386465709
1992 1992 c, g dbSNP:17174788
2026 2026 a, g dbSNP:545361117
2042 2042 -, t dbSNP:777353151
2055 2055 a, g dbSNP:760382239
2066 2066 a, g dbSNP:752254231
2075 2075 c, t dbSNP:1045619
2114 2114 g, t dbSNP:371037402
2123 2123 g, t dbSNP:578200260

Target ORF information:

RefSeq Version XM_011524959
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011524959

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA TCTTTGGAGG TGGACACCGC CCAGAGGAAG ACGAAGAGGA GGAGATTGTG
ATTGGGTGGC AGGAGAAGCT CTTTAGCCAG TTTGAAGTAG ATCTGTACCA AAATGAAACA
GCCTGTCAGA GTCCTTTGGA TTATCAGTAC CGTCAGGAGA TCCTGAAGCT GGAGAATTCG
GGTGGCAAGA AAAACCGACG AATCTTTACC TACACTGACT CTGATAGATA CACCAATTTG
GAGGAGCACT GTCAGAGAAT GACCACTGCA GCCAGCGAGG TGCCTTCATT CTTGGTCGAG
CGAATGGCAA ATGTCAGGCG TCGCCGGCAG GACAGGCGAG GGATGGAGGG CGGCATCCTC
AAGTCACGCA TCGTCACCTG GGAGCCCTCA GAAGAGTTTG TCAGGAACAA CCACGTCATT
AACACCCCTC TTCAGACAAT GCACATCATG GCAGACCTGG GGCCCTATAA AAAGCTTGGC
TATAAGAAGT ATGAACATGT CCTGTGTACT CTGAAGGTGG ATAGCAATGG TGTGATCACA
GTAAAGCCTG ACTTCACGGG CCTCAAAGGA CCCTACAGGA TTGAGACGGA GGGGGAGAAG
CAGGAGCTGT GGAAATATAC GATCGACAAT GTTTCCCCCC ACGCACAGCC GGAGGAGGAG
GAGCGGGAAC GGCGAGTGTT CAAGGATCTT TATGGCCGGC ACAAGGAGTA TCTCAGCAGC
CTCGTAGGCA CCGACTTTGA GATGACTGTC CCAGGTGCCC TCCGGCTCTT TGTAAATGGA
GAGGTCGTTT CAGCCCAAGG CTATGAGTAT GACAATCTCT ACGTCCACTT CTTTGTAGAA
TTGCCAACTG CTCACTGGTC AAGCCCAGCA TTCCAGCAGC TCTCAGGAGT AACACAGACC
TGCACCACCA AGTCCCTGGC AATGGACAAG GTGGCTCACT TCTCCTACCC ATTCACGTTT
GAAGCCTTCT TCCTCCATGA GGATGAATCT TCTGATGCAC TCCCGGAGTG GCCTGTGCTC
TACTGTGAGG TCCTCTCGCT GGACTTCTGG CAGAGGTACC GTGTGGAAGG CTATGGGGCT
GTGGTGCTGC CTGCCACTCC AGGGCCTTCA TGGAATCGAG CTCCCTTCAG AAAAGGATGC
GGAGTGTGTT GGACCGTCTG GAAGGGTTCA GCCAGCAGAG TTCCATTCAC AATGTGCTAG

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu68761D
Sequence Information ORF Nucleotide Sequence (Length: 1308bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product Meckel syndrome type 1 protein isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA TCTTTGGAGG TGGACACCGC CCAGAGGAAG ACGAAGAGGA GGAGATTGTG
ATTGGGTGGC AGGAGAAGCT CTTTAGCCAG TTTGAAGTAG ATCTGTACCA AAATGAAACA
GCCTGTCAGA GTCCTTTGGA TTATCAGTAC CGTCAGGAGA TCCTGAAGCT GGAGAATTCG
GGTGGCAAGA AAAACCGACG AATCTTTACC TACACTGACT CTGATAGATA CACCAATTTG
GAGGAGCACT GTCAGAGAAT GACCACTGCA GCCAGCGAGG TGCCTTCATT CTTGGTCGAG
CGAATGGCAA ATGTCAGGCG TCGCCGGCAG GACAGGCGAG GGATGGAGGG CGGCATCCTC
AAGTCACGCA TCGTCACCTG GGAGCCCTCA GAAGAGTTTG TCAGGAACAA CCACGTCATT
AACACCCCTC TTCAGACAAT GCACATCATG GCAGACCTGG GGCCCTATAA AAAGCTTGGC
TATAAGAAGT ATGAACATGT CCTGTGTACT CTGAAGGTGG ATAGCAATGG TGTGATCACA
GTAAAGCCTG ACTTCACGGG CCTCAAAGGA CCCTACAGGA TTGAGACGGA GGGGGAGAAG
CAGGAGCTGT GGAAATATAC GATCGACAAT GTTTCCCCCC ACGCACAGCC GGAGGAGGAG
GAGCGGGAAC GGCGAGTGTT CAAGGATCTT TATGGCCGGC ACAAGGAGTA TCTCAGCAGC
CTCGTAGGCA CCGACTTTGA GATGACTGTC CCAGGTGCCC TCCGGCTCTT TGTAAATGGA
GAGGTCGTTT CAGCCCAAGG CTATGAGTAT GACAATCTCT ACGTCCACTT CTTTGTAGAA
TTGCCAACTG CTCACTGGTC AAGCCCAGCA TTCCAGCAGC TCTCAGGAGT AACACAGACC
TGCACCACCA AGTCCCTGGC AATGGACAAG GTGGCTCACT TCTCCTACCC ATTCACGTTT
GAAGCCTTCT TCCTCCATGA GGATGAATCT TCTGATGCAC TCCCGGAGTG GCCTGTGCTC
TACTGTGAGG TCCTCTCGCT GGACTTCTGG CAGAGGTACC GTGTGGAAGG CTATGGGGCT
GTGGTGCTGC CTGCCACTCC AGCGACAATG ACGTGGCCTT GGCACTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011523262.1
CDS49..1356
Misc Feature(1)991..>1329(+)
Translation
Position Chain Variation Link
4 4 a, c dbSNP:777068143
8 8 a, c dbSNP:761615481
9 9 a, g, t dbSNP:760700958
14 14 g, t dbSNP:775593405
15 15 c, t dbSNP:772383483
29 29 c, g, t dbSNP:115507363
31 31 c, g dbSNP:116514023
34 34 c, g dbSNP:541448010
48 48 c, g dbSNP:573940561
53 53 c, t dbSNP:377040370
60 60 c, t dbSNP:372471169
63 63 c, t dbSNP:780539112
71 71 c, t dbSNP:754729482
75 75 c, t dbSNP:369488349
78 78 c, g, t dbSNP:576239597
79 79 a, g dbSNP:762103206
80 80 c, g dbSNP:375223375
100 100 a, c dbSNP:557896607
102 102 -, cccgg dbSNP:730880323
103 103 -, ccggg dbSNP:386834051
118 118 c, t dbSNP:538371478
131 131 c, t dbSNP:201957874
140 140 a, g dbSNP:767646864
149 149 c, t dbSNP:759465861
158 158 a, g, t dbSNP:587779734
162 162 c, t dbSNP:375861391
163 163 c, t dbSNP:11653070
166 166 c, t dbSNP:199832333
183 183 c, g dbSNP:766174298
184 184 a, g dbSNP:183617764
194 194 a, g dbSNP:748133097
196 196 a, g dbSNP:776363123
199 199 c, t dbSNP:368169919
203 203 c, t dbSNP:746450191
212 212 c, t dbSNP:779521346
213 213 c, t dbSNP:375999060
232 232 -, actgcca dbSNP:386834046
232 232 a, g dbSNP:758090787
242 242 g, t dbSNP:748412372
247 247 a, g dbSNP:201362733
248 248 a, g dbSNP:755502660
253 253 c, t dbSNP:756716716
256 256 c, t dbSNP:200340896
257 257 a, g dbSNP:780293222
261 261 a, t dbSNP:557752726
265 265 a, g dbSNP:750702019
267 267 a, g dbSNP:778759498
270 270 c, g dbSNP:142813109
271 271 a, g dbSNP:753620277
280 280 a, g dbSNP:763949111
282 282 c, g, t dbSNP:775351056
290 290 g, t dbSNP:786204222
291 291 c, t dbSNP:771577659
292 292 a, g dbSNP:759008278
293 293 g, t dbSNP:774076532
306 306 a, g dbSNP:62636631
326 326 c, t dbSNP:367668056
333 333 a, g dbSNP:747325433
336 336 c, t dbSNP:187870415
337 337 a, c dbSNP:76838735
339 339 a, g dbSNP:772742739
343 343 a, g dbSNP:76192155
365 365 c, t dbSNP:201574278
370 370 c, t dbSNP:778921113
379 379 c, t dbSNP:201476674
380 380 a, g dbSNP:200331553
402 402 g, t dbSNP:777934536
407 407 c, t dbSNP:755927059
408 408 a, g dbSNP:370781583
416 416 a, g dbSNP:767269565
417 417 a, g, t dbSNP:751455442
422 422 a, c dbSNP:765809969
424 424 -, c dbSNP:775043799
424 424 c, t dbSNP:762482919
425 425 a, g dbSNP:202112856
428 428 a, g dbSNP:765026950
438 438 c, g dbSNP:761521921
439 439 c, t dbSNP:75699185
440 440 a, g dbSNP:776068083
442 442 a, g dbSNP:374506610
449 449 -, ct dbSNP:386834047
456 456 a, g dbSNP:746378310
458 458 a, g dbSNP:747461404
468 468 a, g dbSNP:770948877
471 471 g, t dbSNP:749341221
474 474 a, g dbSNP:386834048
478 478 g, t dbSNP:200332126
481 481 c, t dbSNP:386834049
484 484 a, g dbSNP:185130897
485 485 g, t dbSNP:758424317
504 504 c, t dbSNP:750376903
509 509 c, t dbSNP:778584579
517 517 c, t dbSNP:576624384
518 518 c, t dbSNP:753579811
522 522 a, g dbSNP:763755799
525 525 c, t dbSNP:199684837
526 526 a, g dbSNP:200970148
527 527 a, g dbSNP:766648988
529 529 c, t dbSNP:386834050
530 530 g, t dbSNP:373471917
533 533 c, t dbSNP:370013310
535 535 a, g dbSNP:761830955
538 538 a, g dbSNP:776666925
547 547 c, t dbSNP:370568445
548 548 a, g dbSNP:200149256
550 550 c, t dbSNP:779953982
551 551 a, g dbSNP:771709346
553 553 c, t dbSNP:201845154
555 555 g, t dbSNP:778956664
565 565 c, t dbSNP:756853299
566 566 a, g dbSNP:369388608
579 579 c, t dbSNP:780657802
580 580 a, g dbSNP:754601373
581 581 g, t dbSNP:527350238
590 590 -, a dbSNP:769896408
595 595 c, t dbSNP:559966703
596 596 a, g, t dbSNP:145764409
598 598 a, g dbSNP:764242484
601 601 a, g dbSNP:200185068
605 605 c, t dbSNP:775805558
610 610 a, g dbSNP:373755286
611 611 a, g dbSNP:571769092
637 637 a, g dbSNP:759802849
642 642 c, t dbSNP:200628275
643 643 a, g dbSNP:140677541
675 675 a, c, g dbSNP:772982926
690 690 g, t dbSNP:769368392
691 691 a, c dbSNP:747659750
694 694 g, t dbSNP:780721543
695 695 a, g dbSNP:754689401
696 696 c, t dbSNP:746647549
705 705 g, t dbSNP:769659359
710 710 a, g dbSNP:201758855
724 724 c, t dbSNP:776172667
729 729 c, t dbSNP:200531517
730 730 c, t dbSNP:370632053
750 750 c, t dbSNP:777474025
757 757 g, t dbSNP:746666115
761 761 c, t dbSNP:557691735
770 770 g, t dbSNP:376785849
776 776 a, c dbSNP:771310059
785 785 c, t dbSNP:749668169
786 786 a, g, t dbSNP:373491923
789 789 c, g dbSNP:753234130
793 793 -, aaag dbSNP:781397982
814 814 a, g dbSNP:745531071
815 815 c, t dbSNP:778058178
816 816 a, g dbSNP:770140009
819 819 a, g dbSNP:748670448
820 820 a, c, g, t dbSNP:201237547
823 823 a, g dbSNP:780276924
823 823 -, g dbSNP:751818062
848 848 c, t dbSNP:561482424
852 852 c, t dbSNP:750597104
853 853 a, g dbSNP:764943455
857 857 a, g dbSNP:757032985
858 858 c, t dbSNP:753798549
861 861 c, t dbSNP:764069453
862 862 g, t dbSNP:567771507
866 866 a, c dbSNP:368751106
868 868 a, c, g, t dbSNP:201771125
870 870 a, c, t dbSNP:201961765
878 878 c, t dbSNP:770088105
879 879 a, g dbSNP:375557871
880 880 a, g dbSNP:777103638
891 891 -, gag dbSNP:780100856
892 892 c, t dbSNP:189255737
893 893 a, g dbSNP:747504986
898 898 c, t dbSNP:780007842
902 902 a, g dbSNP:184444815
906 906 a, g dbSNP:746105434
910 910 a, c dbSNP:779149583
912 912 a, g dbSNP:757400825
914 914 a, g dbSNP:151023718
916 916 c, t dbSNP:755916212
925 925 c, t dbSNP:777558323
926 926 a, g dbSNP:755840522
927 927 g, t dbSNP:769489826
931 931 a, g dbSNP:201845569
942 942 c, t dbSNP:781136688
949 949 c, t dbSNP:78774579
951 951 c, t dbSNP:754839039
952 952 a, g dbSNP:751146857
953 953 c, t dbSNP:765793774
960 960 c, t dbSNP:142805406
961 961 a, g dbSNP:750025608
966 966 c, t dbSNP:764581050
972 972 a, g dbSNP:761070122
973 973 a, g dbSNP:761528251
975 975 c, t dbSNP:373269341
991 991 c, t dbSNP:767763740
992 992 c, g dbSNP:369483945
1000 1000 g, t dbSNP:774654581
1001 1001 a, t dbSNP:771030303
1010 1010 a, g dbSNP:762973500
1011 1011 g, t dbSNP:773255938
1014 1014 c, t dbSNP:770067106
1015 1015 a, g dbSNP:386834053
1028 1028 a, g dbSNP:776673480
1034 1034 a, g dbSNP:768515749
1050 1050 c, t dbSNP:552668070
1051 1051 a, g dbSNP:775391594
1059 1059 -, ctt dbSNP:765577615
1064 1064 a, t dbSNP:772205041
1066 1066 a, g dbSNP:745789469
1071 1071 a, g dbSNP:201998680
1085 1085 a, c, g dbSNP:567026924
1088 1088 c, g dbSNP:760971749
1093 1093 c, t dbSNP:538164532
1095 1095 a, g dbSNP:775485640
1103 1103 a, g dbSNP:375559691
1105 1105 c, g, t dbSNP:386834041
1115 1115 a, g dbSNP:771939064
1120 1120 a, g dbSNP:745809472
1123 1123 c, t dbSNP:786205508
1127 1127 c, t dbSNP:774391311
1128 1128 -, c dbSNP:762377424
1135 1135 a, g dbSNP:770497568
1143 1143 c, t dbSNP:748800331
1146 1146 a, g dbSNP:777308590
1147 1147 c, g dbSNP:769389013
1150 1150 a, g dbSNP:750926734
1159 1159 a, g dbSNP:762800635
1169 1169 -, tct dbSNP:587777804
1172 1172 -, cct dbSNP:754279998
1178 1178 c, g dbSNP:773157492
1180 1180 a, t dbSNP:769312467
1184 1184 c, t dbSNP:200350173
1185 1185 a, g dbSNP:369141016
1191 1191 a, g dbSNP:77365082
1194 1194 a, c dbSNP:746657618
1195 1195 g, t dbSNP:774595103
1201 1201 c, t dbSNP:771262017
1223 1223 a, t dbSNP:373004412
1232 1232 c, t dbSNP:763534380
1233 1233 a, g, t dbSNP:775169308
1253 1253 c, g, t dbSNP:144764478
1263 1263 c, t dbSNP:202040080
1265 1265 c, g dbSNP:773684291
1266 1266 a, g dbSNP:770493819
1270 1270 a, g dbSNP:372554696
1274 1274 -, t dbSNP:758918957
1279 1279 c, t dbSNP:781423785
1284 1284 a, g dbSNP:755065589
1286 1286 a, g dbSNP:774267957
1288 1288 c, t dbSNP:201036775
1289 1289 a, c, g dbSNP:200679238
1290 1290 c, t dbSNP:201128765
1301 1301 a, g dbSNP:765002709
1317 1317 a, c, g, t dbSNP:749099410
1324 1324 a, g dbSNP:763627565
1325 1325 c, t dbSNP:760184188
1326 1326 c, t dbSNP:772818256
1330 1330 c, g dbSNP:774840755
1331 1331 c, t dbSNP:775651935
1347 1347 g, t dbSNP:771976997
1404 1404 -, g dbSNP:34696633
1431 1431 a, g dbSNP:543976818
1455 1455 a, g dbSNP:755454547
1487 1487 c, g dbSNP:758543840

Target ORF information:

RefSeq Version XM_011524960
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011524960

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA TCTTTGGAGG TGGACACCGC CCAGAGGAAG ACGAAGAGGA GGAGATTGTG
ATTGGGTGGC AGGAGAAGCT CTTTAGCCAG TTTGAAGTAG ATCTGTACCA AAATGAAACA
GCCTGTCAGA GTCCTTTGGA TTATCAGTAC CGTCAGGAGA TCCTGAAGCT GGAGAATTCG
GGTGGCAAGA AAAACCGACG AATCTTTACC TACACTGACT CTGATAGATA CACCAATTTG
GAGGAGCACT GTCAGAGAAT GACCACTGCA GCCAGCGAGG TGCCTTCATT CTTGGTCGAG
CGAATGGCAA ATGTCAGGCG TCGCCGGCAG GACAGGCGAG GGATGGAGGG CGGCATCCTC
AAGTCACGCA TCGTCACCTG GGAGCCCTCA GAAGAGTTTG TCAGGAACAA CCACGTCATT
AACACCCCTC TTCAGACAAT GCACATCATG GCAGACCTGG GGCCCTATAA AAAGCTTGGC
TATAAGAAGT ATGAACATGT CCTGTGTACT CTGAAGGTGG ATAGCAATGG TGTGATCACA
GTAAAGCCTG ACTTCACGGG CCTCAAAGGA CCCTACAGGA TTGAGACGGA GGGGGAGAAG
CAGGAGCTGT GGAAATATAC GATCGACAAT GTTTCCCCCC ACGCACAGCC GGAGGAGGAG
GAGCGGGAAC GGCGAGTGTT CAAGGATCTT TATGGCCGGC ACAAGGAGTA TCTCAGCAGC
CTCGTAGGCA CCGACTTTGA GATGACTGTC CCAGGTGCCC TCCGGCTCTT TGTAAATGGA
GAGGTCGTTT CAGCCCAAGG CTATGAGTAT GACAATCTCT ACGTCCACTT CTTTGTAGAA
TTGCCAACTG CTCACTGGTC AAGCCCAGCA TTCCAGCAGC TCTCAGGAGT AACACAGACC
TGCACCACCA AGTCCCTGGC AATGGACAAG GTGGCTCACT TCTCCTACCC ATTCACGTTT
GAAGCCTTCT TCCTCCATGA GGATGAATCT TCTGATGCAC TCCCGGAGTG GCCTGTGCTC
TACTGTGAGG TCCTCTCGCT GGACTTCTGG CAGAGGTACC GTGTGGAAGG CTATGGGGCT
GTGGTGCTGC CTGCCACTCC AGCGACAATG ACGTGGCCTT GGCACTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu50506D
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product Meckel syndrome type 1 protein isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578831111. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGACCACTG CAGCCAGCGA GGTGCCTTCA TTCTTGGTCG AGCGAATGGC AAATGTCAGG 
CGTCGCCGGC AGGACAGGCG AGGGATGGAG GGCGGCATCC TCAAGTCACG CATCGTCACC
TGGGAGCCCT CAGAAGAGTT TGTCAGGAAC AACCACGTCA TTAACACCCC TCTTCAGACA
ATGCACATCA TGGCAGACCT GGGGCCCTAT AAAAAGCTTG GCTATAAGAA GTATGAACAT
GTCCTGTGTA CTCTGAAGGT GGATAGCAAT GGTGTGATCA CAGTAAAGCC TGACTTCACG
GGCCTCAAAG GACCCTACAG GATTGAGACG GAGGGGGAGA AGCAGGAGCT GTGGAAATAT
ACGATCGACA ATGTTTCCCC CCACGCACAG CCGGAGGAGG AGGAGCGGGA ACGGCGAGTG
TTCAAGGATC TTTATGGCCG GCACAAGGAG TATCTCAGCA GCCTCGTAGG CACCGACTTT
GAGATGACTG TCCCAGGTGC CCTCCGGCTC TTTGTAAATG GAGAGGTCGT TTCAGCCCAA
GGCTATGAGT ATGACAATCT CTACGTCCAC TTCTTTGTAG AATTGCCAAC TGCTCACTGG
TCAAGCCCAG CATTCCAGCA GCTCTCAGGA GTAACACAGA CCTGCACCAC CAAGTCCCTG
GCAATGGACA AGGTGGCTCA CTTCTCCTAC CCATTCACGT TTGAAGCCTT CTTCCTCCAT
GAGGATGAAT CTTCTGATGC ACTCCCGGAG TGGCCTGTGC TCTACTGTGA GGTCCTCTCG
CTGGACTTCT GGCAGAGGTA CCGTGTGGAA GGCTATGGGG CTGTGGTGCT GCCTGCCACT
CCAGGCTCAC ACACCCTGAC AGTCTCCACG TGGAGACCTG TGGAGCTTGG CACGGTGGCT
GAGCTGAGGA GGTTTTTCAT TGGCGGTTCT CTGGAACTGG AGGACCTCTC CTATGTACGG
ATACCAGGAT CCTTCAAGGG CCTTCATGGA ATCGAGCTCC CTTCAGAAAA GGATGCGGAG
TGTGTTGGAC CGTCTGGAAG GGTTCAGCCA GCAGAGTTCC ATTCACAATG TGCTAGAGGC
CTTCCGTCGA GCCCGGCGCC GCATGCAGGA GGCCCGGGAA AGCCTCCCGC AGGACCTAGT
GAGCCCCTCT GGAACCCTGG TCTCCTAGCT CACAGCAGCC CTGGCCCACA GTGCAAGAGG
ACAAGATGGG GGATATCTGA GGCCAGTGCT CTCCTGCCTC TTCGTCTTTC TGATTAG
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005257542.1
CDS486..1742
Misc Feature(1)990..1472(+)
Translation
Position Chain Variation Link
1 1 c, t dbSNP:377040370
8 8 c, t dbSNP:372471169
11 11 c, t dbSNP:780539112
19 19 c, t dbSNP:754729482
23 23 c, t dbSNP:369488349
26 26 c, g, t dbSNP:576239597
27 27 a, g dbSNP:762103206
28 28 c, g dbSNP:375223375
48 48 a, c dbSNP:557896607
50 50 -, cccgg dbSNP:730880323
51 51 -, ccggg dbSNP:386834051
66 66 c, t dbSNP:538371478
79 79 c, t dbSNP:201957874
88 88 a, g dbSNP:767646864
97 97 c, t dbSNP:759465861
106 106 a, g, t dbSNP:587779734
110 110 c, t dbSNP:375861391
111 111 c, t dbSNP:11653070
114 114 c, t dbSNP:199832333
131 131 c, g dbSNP:766174298
132 132 a, g dbSNP:183617764
142 142 a, g dbSNP:748133097
144 144 a, g dbSNP:776363123
147 147 c, t dbSNP:368169919
151 151 c, t dbSNP:746450191
160 160 c, t dbSNP:779521346
161 161 c, t dbSNP:375999060
180 180 -, actgcca dbSNP:386834046
180 180 a, g dbSNP:758090787
187 187 a, g dbSNP:755502660
192 192 c, t dbSNP:756716716
195 195 c, t dbSNP:200340896
196 196 a, g dbSNP:780293222
200 200 a, t dbSNP:557752726
204 204 a, g dbSNP:750702019
206 206 a, g dbSNP:778759498
209 209 c, g dbSNP:142813109
210 210 a, g dbSNP:753620277
219 219 a, g dbSNP:763949111
221 221 c, g, t dbSNP:775351056
229 229 g, t dbSNP:786204222
230 230 c, t dbSNP:771577659
231 231 a, g dbSNP:759008278
232 232 g, t dbSNP:774076532
245 245 a, g dbSNP:62636631
265 265 c, t dbSNP:367668056
272 272 a, g dbSNP:747325433
275 275 c, t dbSNP:187870415
276 276 a, c dbSNP:76838735
278 278 a, g dbSNP:772742739
282 282 a, g dbSNP:76192155
304 304 c, t dbSNP:201574278
309 309 c, t dbSNP:778921113
318 318 c, t dbSNP:201476674
319 319 a, g dbSNP:200331553
341 341 g, t dbSNP:777934536
346 346 c, t dbSNP:755927059
347 347 a, g dbSNP:370781583
355 355 a, g dbSNP:767269565
356 356 a, g, t dbSNP:751455442
361 361 a, c dbSNP:765809969
363 363 -, c dbSNP:775043799
363 363 c, t dbSNP:762482919
364 364 a, g dbSNP:202112856
367 367 a, g dbSNP:765026950
377 377 c, g dbSNP:761521921
378 378 c, t dbSNP:75699185
379 379 a, g dbSNP:776068083
381 381 a, g dbSNP:374506610
388 388 -, ct dbSNP:386834047
395 395 a, g dbSNP:746378310
397 397 a, g dbSNP:747461404
407 407 a, g dbSNP:770948877
410 410 g, t dbSNP:749341221
413 413 a, g dbSNP:386834048
423 423 c, t dbSNP:771519600
424 424 c, t dbSNP:375727823
427 427 c, t dbSNP:201966188
428 428 a, g dbSNP:769805866
446 446 c, t dbSNP:370458403
447 447 a, g, t dbSNP:375851052
449 449 -, a dbSNP:773819704
459 459 c, g dbSNP:750031301
464 464 -, c dbSNP:770779646
472 472 a, g dbSNP:746867953
477 477 g, t dbSNP:200332126
480 480 c, t dbSNP:386834049
483 483 a, g dbSNP:185130897
484 484 g, t dbSNP:758424317
503 503 c, t dbSNP:750376903
508 508 c, t dbSNP:778584579
516 516 c, t dbSNP:576624384
517 517 c, t dbSNP:753579811
521 521 a, g dbSNP:763755799
524 524 c, t dbSNP:199684837
525 525 a, g dbSNP:200970148
526 526 a, g dbSNP:766648988
528 528 c, t dbSNP:386834050
529 529 g, t dbSNP:373471917
532 532 c, t dbSNP:370013310
534 534 a, g dbSNP:761830955
537 537 a, g dbSNP:776666925
546 546 c, t dbSNP:370568445
547 547 a, g dbSNP:200149256
549 549 c, t dbSNP:779953982
550 550 a, g dbSNP:771709346
552 552 c, t dbSNP:201845154
554 554 g, t dbSNP:778956664
564 564 c, t dbSNP:756853299
565 565 a, g dbSNP:369388608
578 578 c, t dbSNP:780657802
579 579 a, g dbSNP:754601373
580 580 g, t dbSNP:527350238
589 589 -, a dbSNP:769896408
594 594 c, t dbSNP:559966703
595 595 a, g, t dbSNP:145764409
597 597 a, g dbSNP:764242484
600 600 a, g dbSNP:200185068
604 604 c, t dbSNP:775805558
609 609 a, g dbSNP:373755286
610 610 a, g dbSNP:571769092
636 636 a, g dbSNP:759802849
641 641 c, t dbSNP:200628275
642 642 a, g dbSNP:140677541
674 674 a, c, g dbSNP:772982926
689 689 g, t dbSNP:769368392
690 690 a, c dbSNP:747659750
693 693 g, t dbSNP:780721543
694 694 a, g dbSNP:754689401
695 695 c, t dbSNP:746647549
704 704 g, t dbSNP:769659359
709 709 a, g dbSNP:201758855
723 723 c, t dbSNP:776172667
728 728 c, t dbSNP:200531517
729 729 c, t dbSNP:370632053
749 749 c, t dbSNP:777474025
756 756 g, t dbSNP:746666115
760 760 c, t dbSNP:557691735
769 769 g, t dbSNP:376785849
775 775 a, c dbSNP:771310059
784 784 c, t dbSNP:749668169
785 785 a, g, t dbSNP:373491923
788 788 c, g dbSNP:753234130
792 792 -, aaag dbSNP:781397982
813 813 a, g dbSNP:745531071
814 814 c, t dbSNP:778058178
815 815 a, g dbSNP:770140009
818 818 a, g dbSNP:748670448
819 819 a, c, g, t dbSNP:201237547
822 822 a, g dbSNP:780276924
822 822 -, g dbSNP:751818062
847 847 c, t dbSNP:561482424
851 851 c, t dbSNP:750597104
852 852 a, g dbSNP:764943455
856 856 a, g dbSNP:757032985
857 857 c, t dbSNP:753798549
860 860 c, t dbSNP:764069453
861 861 g, t dbSNP:567771507
865 865 a, c dbSNP:368751106
867 867 a, c, g, t dbSNP:201771125
869 869 a, c, t dbSNP:201961765
877 877 c, t dbSNP:770088105
878 878 a, g dbSNP:375557871
879 879 a, g dbSNP:777103638
890 890 -, gag dbSNP:780100856
891 891 c, t dbSNP:189255737
892 892 a, g dbSNP:747504986
897 897 c, t dbSNP:780007842
901 901 a, g dbSNP:184444815
905 905 a, g dbSNP:746105434
909 909 a, c dbSNP:779149583
911 911 a, g dbSNP:757400825
913 913 a, g dbSNP:151023718
915 915 c, t dbSNP:755916212
924 924 c, t dbSNP:777558323
925 925 a, g dbSNP:755840522
926 926 g, t dbSNP:769489826
930 930 a, g dbSNP:201845569
941 941 c, t dbSNP:781136688
948 948 c, t dbSNP:78774579
950 950 c, t dbSNP:754839039
951 951 a, g dbSNP:751146857
952 952 c, t dbSNP:765793774
959 959 c, t dbSNP:142805406
960 960 a, g dbSNP:750025608
965 965 c, t dbSNP:764581050
971 971 a, g dbSNP:761070122
972 972 a, g dbSNP:761528251
974 974 c, t dbSNP:373269341
990 990 c, t dbSNP:767763740
991 991 c, g dbSNP:369483945
999 999 g, t dbSNP:774654581
1000 1000 a, t dbSNP:771030303
1009 1009 a, g dbSNP:762973500
1010 1010 g, t dbSNP:773255938
1013 1013 c, t dbSNP:770067106
1014 1014 a, g dbSNP:386834053
1027 1027 a, g dbSNP:776673480
1033 1033 a, g dbSNP:768515749
1049 1049 c, t dbSNP:552668070
1050 1050 a, g dbSNP:775391594
1058 1058 -, ctt dbSNP:765577615
1063 1063 a, t dbSNP:772205041
1065 1065 a, g dbSNP:745789469
1070 1070 a, g dbSNP:201998680
1084 1084 a, c, g dbSNP:567026924
1087 1087 c, g dbSNP:760971749
1092 1092 c, t dbSNP:538164532
1094 1094 a, g dbSNP:775485640
1102 1102 a, g dbSNP:375559691
1104 1104 c, g, t dbSNP:386834041
1114 1114 a, g dbSNP:771939064
1119 1119 a, g dbSNP:745809472
1122 1122 c, t dbSNP:786205508
1126 1126 c, t dbSNP:774391311
1127 1127 -, c dbSNP:762377424
1134 1134 a, g dbSNP:770497568
1142 1142 c, t dbSNP:748800331
1145 1145 a, g dbSNP:777308590
1146 1146 c, g dbSNP:769389013
1149 1149 a, g dbSNP:750926734
1158 1158 a, g dbSNP:762800635
1168 1168 -, tct dbSNP:587777804
1171 1171 -, cct dbSNP:754279998
1177 1177 c, g dbSNP:773157492
1179 1179 a, t dbSNP:769312467
1183 1183 c, t dbSNP:200350173
1184 1184 a, g dbSNP:369141016
1190 1190 a, g dbSNP:77365082
1193 1193 a, c dbSNP:746657618
1194 1194 g, t dbSNP:774595103
1200 1200 c, t dbSNP:771262017
1222 1222 a, t dbSNP:373004412
1231 1231 c, t dbSNP:763534380
1232 1232 a, g, t dbSNP:775169308
1252 1252 c, g, t dbSNP:144764478
1262 1262 c, t dbSNP:202040080
1264 1264 c, g dbSNP:773684291
1265 1265 a, g dbSNP:770493819
1269 1269 a, g dbSNP:372554696
1273 1273 -, t dbSNP:758918957
1278 1278 c, t dbSNP:781423785
1283 1283 a, g dbSNP:755065589
1285 1285 a, g dbSNP:774267957
1287 1287 c, t dbSNP:201036775
1288 1288 a, c, g dbSNP:200679238
1289 1289 c, t dbSNP:201128765
1300 1300 a, g dbSNP:765002709
1316 1316 a, c, g, t dbSNP:749099410
1323 1323 a, g dbSNP:763627565
1324 1324 c, t dbSNP:760184188
1325 1325 c, t dbSNP:772818256
1329 1329 c, g dbSNP:774840755
1335 1335 c, t dbSNP:141741656
1340 1340 c, t dbSNP:761247194
1343 1343 a, c, g dbSNP:555977738
1350 1350 c, t dbSNP:759662552
1351 1351 c, t dbSNP:774384333
1354 1354 c, t dbSNP:755841031
1355 1355 a, g dbSNP:749503564
1374 1374 a, g dbSNP:773075697
1378 1378 c, t dbSNP:367625961
1379 1379 a, c, g dbSNP:781038098
1381 1381 g, t dbSNP:754930606
1382 1382 a, g dbSNP:569499621
1388 1388 a, g dbSNP:746581223
1396 1396 a, g dbSNP:779697924
1405 1405 c, t dbSNP:200865108
1409 1409 a, c, t dbSNP:764549118
1410 1410 a, g dbSNP:538819956
1411 1411 -, gtg dbSNP:373382151
1412 1412 g, t dbSNP:753085014
1419 1419 c, g dbSNP:199927741
1435 1435 c, t dbSNP:760032525
1438 1438 a, g dbSNP:730882120
1439 1439 c, t dbSNP:774475723
1443 1443 c, t dbSNP:766392300
1444 1444 a, g dbSNP:201619500
1445 1445 g, t dbSNP:773269657
1470 1470 -, c dbSNP:780161503
1471 1471 a, g dbSNP:144635826
1478 1478 c, g, t dbSNP:758838271
1479 1479 a, g dbSNP:368535131
1498 1498 a, g dbSNP:778890248
1501 1501 c, t dbSNP:757310695
1502 1502 a, g dbSNP:373843986
1504 1504 a, g dbSNP:9906421
1507 1507 a, g dbSNP:370130538
1516 1516 c, t dbSNP:775558298
1517 1517 a, g, t dbSNP:200658872
1526 1526 a, g dbSNP:767285168
1544 1544 c, g dbSNP:759400183
1553 1553 a, g dbSNP:774309110
1563 1563 g, t dbSNP:767250536
1565 1565 a, c, g dbSNP:751294519
1570 1570 c, t dbSNP:745946583
1571 1571 a, g, t dbSNP:779093781
1573 1573 a, c dbSNP:772719574
1574 1574 a, g dbSNP:199910690
1575 1575 a, c dbSNP:761483576
1578 1578 c, g dbSNP:35493987
1579 1579 c, t dbSNP:768171144
1580 1580 a, g dbSNP:746283445
1582 1582 c, t dbSNP:35464956
1583 1583 a, g dbSNP:771624307
1585 1585 c, t dbSNP:34631184
1586 1586 a, g, t dbSNP:557678962
1593 1593 a, g dbSNP:267604965
1600 1600 c, t dbSNP:748406509
1602 1602 a, g dbSNP:781530535
1607 1607 a, g dbSNP:755128244
1608 1608 a, c dbSNP:545364645
1612 1612 c, t dbSNP:751425104
1613 1613 c, t dbSNP:771585740
1614 1614 a, g dbSNP:758334200
1626 1626 c, g dbSNP:34092089
1634 1634 c, g dbSNP:764747820
1641 1641 c, t dbSNP:553314050
1644 1644 a, c, g dbSNP:11548967
1647 1647 c, t dbSNP:376925280
1656 1656 c, t dbSNP:774792843
1663 1663 g, t dbSNP:771356715
1664 1664 c, t dbSNP:763587314
1680 1680 a, g dbSNP:566239288
1691 1691 -, a dbSNP:34304018
1695 1695 a, g dbSNP:773809071
1696 1696 a, g dbSNP:369146407
1702 1702 a, c dbSNP:371962780
1711 1711 c, g dbSNP:35384582
1728 1728 c, t dbSNP:35184412
1749 1749 c, t dbSNP:568504276
1750 1750 a, g dbSNP:550885201
1787 1787 c, t dbSNP:34224064
1788 1788 a, g dbSNP:565533857
1805 1805 a, g dbSNP:780840500
1808 1808 c, t dbSNP:754562608
1866 1866 c, t dbSNP:751103496
1975 1975 g, t dbSNP:185405908
1996 1996 c, g dbSNP:759501344
2001 2001 c, g dbSNP:781131831
2004 2004 a, g dbSNP:528253185
2004 2004 -, g dbSNP:35298266
2019 2019 a, g dbSNP:768932890
2024 2024 a, g dbSNP:370149098
2028 2028 c, g, t dbSNP:200682912
2067 2067 c, t dbSNP:2302313
2068 2068 a, g dbSNP:750194631
2075 2075 a, g dbSNP:778861333
2122 2122 c, t dbSNP:386465709
2125 2125 c, g dbSNP:17174788
2159 2159 a, g dbSNP:545361117
2175 2175 -, t dbSNP:777353151
2188 2188 a, g dbSNP:760382239
2199 2199 a, g dbSNP:752254231
2208 2208 c, t dbSNP:1045619
2247 2247 g, t dbSNP:371037402
2256 2256 g, t dbSNP:578200260

Target ORF information:

RefSeq Version XM_005257485
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005257485

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGACCACTG CAGCCAGCGA GGTGCCTTCA TTCTTGGTCG AGCGAATGGC AAATGTCAGG 
CGTCGCCGGC AGGACAGGCG AGGGATGGAG GGCGGCATCC TCAAGTCACG CATCGTCACC
TGGGAGCCCT CAGAAGAGTT TGTCAGGAAC AACCACGTCA TTAACACCCC TCTTCAGACA
ATGCACATCA TGGCAGACCT GGGGCCCTAT AAAAAGCTTG GCTATAAGAA GTATGAACAT
GTCCTGTGTA CTCTGAAGGT GGATAGCAAT GGTGTGATCA CAGTAAAGCC TGACTTCACG
GGCCTCAAAG GACCCTACAG GATTGAGACG GAGGGGGAGA AGCAGGAGCT GTGGAAATAT
ACGATCGACA ATGTTTCCCC CCACGCACAG CCGGAGGAGG AGGAGCGGGA ACGGCGAGTG
TTCAAGGATC TTTATGGCCG GCACAAGGAG TATCTCAGCA GCCTCGTAGG CACCGACTTT
GAGATGACTG TCCCAGGTGC CCTCCGGCTC TTTGTAAATG GAGAGGTCGT TTCAGCCCAA
GGCTATGAGT ATGACAATCT CTACGTCCAC TTCTTTGTAG AATTGCCAAC TGCTCACTGG
TCAAGCCCAG CATTCCAGCA GCTCTCAGGA GTAACACAGA CCTGCACCAC CAAGTCCCTG
GCAATGGACA AGGTGGCTCA CTTCTCCTAC CCATTCACGT TTGAAGCCTT CTTCCTCCAT
GAGGATGAAT CTTCTGATGC ACTCCCGGAG TGGCCTGTGC TCTACTGTGA GGTCCTCTCG
CTGGACTTCT GGCAGAGGTA CCGTGTGGAA GGCTATGGGG CTGTGGTGCT GCCTGCCACT
CCAGGCTCAC ACACCCTGAC AGTCTCCACG TGGAGACCTG TGGAGCTTGG CACGGTGGCT
GAGCTGAGGA GGTTTTTCAT TGGCGGTTCT CTGGAACTGG AGGACCTCTC CTATGTACGG
ATACCAGGAT CCTTCAAGGG CCTTCATGGA ATCGAGCTCC CTTCAGAAAA GGATGCGGAG
TGTGTTGGAC CGTCTGGAAG GGTTCAGCCA GCAGAGTTCC ATTCACAATG TGCTAGAGGC
CTTCCGTCGA GCCCGGCGCC GCATGCAGGA GGCCCGGGAA AGCCTCCCGC AGGACCTAGT
GAGCCCCTCT GGAACCCTGG TCTCCTAGCT CACAGCAGCC CTGGCCCACA GTGCAAGAGG
ACAAGATGGG GGATATCTGA GGCCAGTGCT CTCCTGCCTC TTCGTCTTTC TGATTAG

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu50507D
Sequence Information ORF Nucleotide Sequence (Length: 1077bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015