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SDHAF2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol SDHAF2
Entrez Gene ID 54949
Full Name succinate dehydrogenase complex assembly factor 2
Synonyms C11orf79, PGL2, SDH5
General protein information
Preferred Names
succinate dehydrogenase assembly factor 2, mitochondrial
Names
succinate dehydrogenase assembly factor 2, mitochondrial
hSDH5
SDH assembly factor 2
succinate dehydrogenase subunit 5, mitochondrial
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11q12.2

Summary This gene encodes a mitochondrial protein needed for the flavination of a succinate dehydrogenase complex subunit required for activity of the complex. Mutations in this gene are associated with paraganglioma.[provided by RefSeq, Jul 2010]. lac of sum
Disorder MIM:

613019

Disorder Html: Paragangliomas 2, 601650 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_017841 NP_060311 succinate dehydrogenase assembly factor 2, mitochondrial


Homo sapiens (human) SDHAF2 NP_060311.1
Pan troglodytes (chimpanzee) PPP1R32 NP_001238783.1
Macaca mulatta (Rhesus monkey) SDHAF2 XP_001082359.2
Canis lupus familiaris (dog) SDHAF2 XP_533273.2
Bos taurus (cattle) SDHAF2 NP_001070513.1
Mus musculus (house mouse) Sdhaf2 NP_079609.2
Rattus norvegicus (Norway rat) Sdhaf2 NP_001008372.1
Gallus gallus (chicken) LOC426015 NP_001264818.1
Danio rerio (zebrafish) sdhaf2 NP_001076333.1
Drosophila melanogaster (fruit fly) CG14757 NP_610363.1
Drosophila melanogaster (fruit fly) CG12895 NP_001260868.1
Xenopus (Silurana) tropicalis (western clawed frog) sdhaf2 XP_002937676.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SDHAF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SDHAF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu22591
NM_017841 Homo sapiens succinate dehydrogenase complex assembly factor 2 (SDHAF2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22591
Clone ID Related Accession (Same CDS sequence) NM_017841
Accession Version NM_017841.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 501bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product succinate dehydrogenase assembly factor 2, mitochondrial
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK000494.1, AI039966.1 and AI660205.1. This sequence is a reference standard in the RefSeqGene project. On Jul 17, 2010 this sequence version replaced gi:8923449. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK000494.1, BC002331.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGGCGGTGT CTACAGTGTT CTCGACTTCG TCGCTGATGC TTGCTCTGTC AAGGCACAGC 
CTATTGTCTC CTTTGCTCAG TGTGACATCA TTCAGACGCT TCTACAGAGG TGACAGCCCA
ACAGATTCCC AAAAGGACAT GATTGAAATC CCTTTGCCTC CATGGCAGGA GAGAACTGAT
GAATCCATAG AAACCAAAAG AGCCCGCCTG CTCTATGAGA GCAGAAAGAG GGGAATGTTG
GAAAACTGCA TTCTTCTTAG TCTTTTTGCT AAAGAACATC TGCAGCACAT GACAGAAAAG
CAGCTGAACC TCTATGACCG CCTGATTAAC GAGCCTAGTA ATGACTGGGA TATTTACTAC
TGGGCCACAG AAGCTAAACC AGCCCCAGAA ATATTTGAAA ATGAAGTCAT GGCCCTGCTG
AGAGACTTTG CTAAAAACAA AAACAAAGAG CAGAGACTGC GTGCCCCAGA TCTTGAGTAC
CTCTTTGAAA AGCCACGTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_060311.1
CDS23..523
Misc Feature(1)218..445(+)
Exon (1)1..58
Gene:SDHAF2
Gene Synonym:
Exon (2)59..282
Gene:SDHAF2
Gene Synonym:
Exon (3)283..392
Gene:SDHAF2
Gene Synonym:
Exon (4)393..1219
Gene:SDHAF2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_017841
Organism Homo sapiens (human)
Definition Homo sapiens succinate dehydrogenase complex assembly factor 2 (SDHAF2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_017841

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGCGGTGT CTACAGTGTT CTCGACTTCG TCGCTGATGC TTGCTCTGTC AAGGCACAGC 
CTATTGTCTC CTTTGCTCAG TGTGACATCA TTCAGACGCT TCTACAGAGG TGACAGCCCA
ACAGATTCCC AAAAGGACAT GATTGAAATC CCTTTGCCTC CATGGCAGGA GAGAACTGAT
GAATCCATAG AAACCAAAAG AGCCCGCCTG CTCTATGAGA GCAGAAAGAG GGGAATGTTG
GAAAACTGCA TTCTTCTTAG TCTTTTTGCT AAAGAACATC TGCAGCACAT GACAGAAAAG
CAGCTGAACC TCTATGACCG CCTGATTAAC GAGCCTAGTA ATGACTGGGA TATTTACTAC
TGGGCCACAG AAGCTAAACC AGCCCCAGAA ATATTTGAAA ATGAAGTCAT GGCCCTGCTG
AGAGACTTTG CTAAAAACAA AAACAAAGAG CAGAGACTGC GTGCCCCAGA TCTTGAGTAC
CTCTTTGAAA AGCCACGTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Universal genetic screening uncovers a novel presentation of an SDHAF2 mutation
J. Clin. Endocrinol. Metab. 99 (7), E1392-E1396 (2014)
Casey R, Garrahy A, Tuthill A, O'Halloran D, Joyce C, Casey MB, O'Shea P and Bell M.


book

Mitochondrial matrix proteostasis is linked to hereditary paraganglioma: LON-mediated turnover of the human flavinylation factor SDH5 is regulated by its interaction with SDHA
FASEB J. 28 (4), 1794-1804 (2014)
Bezawork-Geleta A, Saiyed T, Dougan DA and Truscott KN.


book

Role of SDHAF2 and SDHD in von Hippel-Lindau associated pheochromocytomas
World J Surg 38 (3), 724-732 (2014)
Kugelberg J, Welander J, Schiavi F, Fassina A, Backdahl M, Larsson C, Opocher G, Soderkvist P, Dahia PL, Neumann HP and Gimm O.


book

Succinate dehydrogenase 5 (SDH5) regulates glycogen synthase kinase 3beta-beta-catenin-mediated lung cancer metastasis
J. Biol. Chem. 288 (41), 29965-29973 (2013)
Liu J, Gao L, Zhang H, Wang D, Wang M, Zhu J, Pang C and Wang C.


book

Emerging concepts in the flavinylation of succinate dehydrogenase
Biochim. Biophys. Acta 1827 (5), 627-636 (2013)
Kim HJ and Winge DR.


book

SDH5, a gene required for flavination of succinate dehydrogenase, is mutated in paraganglioma
Science 325 (5944), 1139-1142 (2009)
Hao HX, Khalimonchuk O, Schraders M, Dephoure N, Bayley JP, Kunst H, Devilee P, Cremers CW, Schiffman JD, Bentz BG, Gygi SP, Winge DR, Kremer H and Rutter J.


book

A human protein-protein interaction network: a resource for annotating the proteome
Cell 122 (6), 957-968 (2005)
Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksoz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H and Wanker EE.


book

Fine mapping of an imprinted gene for familial nonchromaffin paragangliomas, on chromosome 11q23
Am. J. Hum. Genet. 60 (1), 121-132 (1997)
Baysal BE, Farr JE, Rubinstein WS, Galus RA, Johnson KA, Aston CE, Myers EN, Johnson JT, Carrau R, Kirkpatrick SJ, Myssiorek D, Singh D, Saha S, Gollin SM, Evans GA, James MR and Richard CW 3rd.


book

Fine mapping of a putatively imprinted gene for familial non-chromaffin paragangliomas to chromosome 11q13.1: evidence for genetic heterogeneity
Hum. Genet. 95 (1), 56-62 (1995)
Mariman EC, van Beersum SE, Cremers CW, Struycken PM and Ropers HH.


book

Hereditary Paraganglioma-Pheochromocytoma Syndromes
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Kirmani,S. and Young,W.F.


 
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