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PPP2R1A cDNA ORF clone, Homo sapiens (human)

Gene Symbol PPP2R1A
Entrez Gene ID 5518
Full Name protein phosphatase 2, regulatory subunit A, alpha
Synonyms MRD36, PP2A-Aalpha, PP2AAALPHA, PR65A
General protein information
Preferred Names
serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
Names
serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
PP2A subunit A isoform R1-alpha
PP2A subunit A isoform PR65-alpha
medium tumor antigen-associated 61 KDA protein
protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform
serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alpha isoform
NP_055040.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19q13.41

Summary This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]. lac of sum
Disorder MIM:

605983

Disorder Html:

mRNA and Protein(s)

mRNA Protein Name
NM_014225 NP_055040 serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform

KEGG
hsa04350 TGF-beta signaling pathway
hsa04530 Tight junction
hsa04730 Long-term depression
hsa04114 Oocyte meiosis
hsa05142 Chagas disease (American trypanosomiasis)
hsa05160 Hepatitis C
hsa03015 mRNA surveillance pathway
hsa04728 Dopaminergic synapse
hsa04151 PI3K-Akt signaling pathway
hsa04390 Hippo signaling pathway
hsa04261 Adrenergic signaling in cardiomyocytes
hsa04152 AMPK signaling pathway
hsa04071 Sphingolipid signaling pathway
Reactome
R-HSA-1852241 Organelle biogenesis and maintenance
R-HSA-5617833 Assembly of the primary cilium
R-HSA-5663202 Diseases of signal transduction
R-HSA-5620912 Anchoring of the basal body to the plasma membrane
R-HSA-1643685 Disease
R-HSA-4839735 AXIN mutants destabilize the destruction complex, activating WNT signaling
R-HSA-5467348 Truncations of AMER1 destabilize the destruction complex
R-HSA-4839743 phosphorylation site mutants of CTNNB1 are not targeted to the proteasome by the destruction complex
R-HSA-5358749 S37 mutants of beta-catenin aren't phosphorylated
R-HSA-4791275 Signaling by WNT in cancer
R-HSA-4839744 truncated APC mutants destabilize the destruction complex
R-HSA-5467337 APC truncation mutants have impaired AXIN binding
R-HSA-5339716 Misspliced GSK3beta mutants stabilize beta-catenin
R-HSA-5467340 AXIN missense mutants destabilize the destruction complex
R-HSA-1280218 Adaptive Immune System
R-HSA-168256 Immune System
R-HSA-4839748 AMER1 mutants destabilize the destruction complex
R-HSA-5358747 S33 mutants of beta-catenin aren't phosphorylated
R-HSA-5358751 S45 mutants of beta-catenin aren't phosphorylated
R-HSA-5358752 T41 mutants of beta-catenin aren't phosphorylated
R-HSA-388841 Costimulation by the CD28 family
R-HSA-389513 CTLA4 inhibitory signaling
R-HSA-202670 ERKs are inactivated
R-HSA-168164 Toll Like Receptor 3 (TLR3) Cascade
R-HSA-198753 ERK/MAPK targets
R-HSA-166166 MyD88-independent TLR3/TLR4 cascade
R-HSA-450294 MAP kinase activation in TLR cascade
R-HSA-975871 MyD88 cascade initiated on plasma membrane
R-HSA-450282 MAPK targets/ Nuclear events mediated by MAP kinases
R-HSA-168176 Toll Like Receptor 5 (TLR5) Cascade
R-HSA-168898 Toll-Like Receptors Cascades
R-HSA-168142 Toll Like Receptor 10 (TLR10) Cascade
R-HSA-975138 TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation
R-HSA-168249 Innate Immune System
R-HSA-937061 TRIF-mediated TLR3/TLR4 signaling
R-HSA-168179 Toll Like Receptor TLR1:TLR2 Cascade
R-HSA-168138 Toll Like Receptor 9 (TLR9) Cascade
R-HSA-181438 Toll Like Receptor 2 (TLR2) Cascade
R-HSA-166016 Toll Like Receptor 4 (TLR4) Cascade
R-HSA-168188 Toll Like Receptor TLR6:TLR2 Cascade
R-HSA-2172127 DAP12 interactions
R-HSA-166058 MyD88:Mal cascade initiated on plasma membrane
R-HSA-168181 Toll Like Receptor 7/8 (TLR7/8) Cascade
R-HSA-975155 MyD88 dependent cascade initiated on endosome
R-HSA-2454202 Fc epsilon receptor (FCERI) signaling
R-HSA-2871796 FCERI mediated MAPK activation
R-HSA-166054 Activated TLR4 signalling
R-HSA-2424491 DAP12 signaling
R-HSA-1280215 Cytokine Signaling in Immune system
R-HSA-5673001 RAF/MAP kinase cascade
R-HSA-5673000 RAF activation
R-HSA-512988 Interleukin-3, 5 and GM-CSF signaling
R-HSA-109582 Hemostasis
R-HSA-5675221 Negative regulation of MAPK pathway
R-HSA-912526 Interleukin receptor SHC signaling
R-HSA-449147 Signaling by Interleukins
R-HSA-451927 Interleukin-2 signaling
R-HSA-418346 Platelet homeostasis
R-HSA-432142 Platelet sensitization by LDL
R-HSA-162582 Signal Transduction
R-HSA-179812 GRB2 events in EGFR signaling
R-HSA-180336 SHC1 events in EGFR signaling
R-HSA-5654693 FRS-mediated FGFR1 signaling
R-HSA-5654687 Downstream signaling of activated FGFR1
R-HSA-177929 Signaling by EGFR
R-HSA-5654726 Negative regulation of FGFR1 signaling
R-HSA-5654738 Signaling by FGFR2
R-HSA-190236 Signaling by FGFR
R-HSA-5654741 Signaling by FGFR3
R-HSA-5654700 FRS-mediated FGFR2 signaling
R-HSA-5654736 Signaling by FGFR1
R-HSA-5654743 Signaling by FGFR4
R-HSA-1295596 Spry regulation of FGF signaling
R-HSA-74751 Insulin receptor signalling cascade
R-HSA-112399 IRS-mediated signalling
R-HSA-112412 SOS-mediated signalling
R-HSA-5654706 FRS-mediated FGFR3 signaling
R-HSA-5654708 Downstream signaling of activated FGFR3
R-HSA-74752 Signaling by Insulin receptor
R-HSA-166520 Signalling by NGF
R-HSA-187037 NGF signalling via TRKA from the plasma membrane
R-HSA-187687 Signalling to ERKs
R-HSA-5654716 Downstream signaling of activated FGFR4
R-HSA-5654696 Downstream signaling of activated FGFR2
R-HSA-5654727 Negative regulation of FGFR2 signaling
R-HSA-5654733 Negative regulation of FGFR4 signaling
R-HSA-5654712 FRS-mediated FGFR4 signaling
R-HSA-186763 Downstream signal transduction
R-HSA-186797 Signaling by PDGF
R-HSA-4420097 VEGFA-VEGFR2 Pathway
R-HSA-169893 Prolonged ERK activation events
R-HSA-170984 ARMS-mediated activation
R-HSA-194138 Signaling by VEGF
R-HSA-170968 Frs2-mediated activation
R-HSA-5654732 Negative regulation of FGFR3 signaling
R-HSA-1250196 SHC1 events in ERBB2 signaling
R-HSA-1433557 Signaling by SCF-KIT
R-HSA-167044 Signalling to RAS
R-HSA-1250347 SHC1 events in ERBB4 signaling
R-HSA-1963640 GRB2 events in ERBB2 signaling
R-HSA-1227986 Signaling by ERBB2
R-HSA-198725 Nuclear Events (kinase and transcription factor activation)
R-HSA-5683057 MAPK family signaling cascades
R-HSA-187706 Signalling to p38 via RIT and RIN
R-HSA-1236394 Signaling by ERBB4
R-HSA-194315 Signaling by Rho GTPases
R-HSA-195258 RHO GTPase Effectors
R-HSA-5218921 VEGFR2 mediated cell proliferation
R-HSA-5684996 MAPK1/MAPK3 signaling
R-HSA-372790 Signaling by GPCR
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-196299 Beta-catenin phosphorylation cascade
R-HSA-201681 TCF dependent signaling in response to WNT
R-HSA-881907 Gastrin-CREB signalling pathway via PKC and MAPK
R-HSA-111885 Opioid Signalling
R-HSA-4641262 Disassembly of the destruction complex and recruitment of AXIN to the membrane
R-HSA-195721 Signaling by Wnt
R-HSA-180024 DARPP-32 events
R-HSA-74160 Gene Expression
R-HSA-195253 Degradation of beta-catenin by the destruction complex
R-HSA-2586552 Signaling by Leptin
R-HSA-2428928 IRS-related events triggered by IGF1R
R-HSA-2404192 Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
R-HSA-927802 Nonsense-Mediated Decay (NMD)
R-HSA-1640170 Cell Cycle
R-HSA-2428924 IGF1R signaling cascade
R-HSA-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)
R-HSA-69278 Cell Cycle, Mitotic
R-HSA-69231 Cyclin D associated events in G1
R-HSA-113510 E2F mediated regulation of DNA replication
R-HSA-453274 Mitotic G2-G2/M phases
R-HSA-69236 G1 Phase
R-HSA-69275 G2/M Transition
R-HSA-380270 Recruitment of mitotic centrosome proteins and complexes
R-HSA-68875 Mitotic Prophase
R-HSA-68886 M Phase
R-HSA-380287 Centrosome maturation
R-HSA-380259 Loss of Nlp from mitotic centrosomes
R-HSA-2555396 Mitotic Metaphase and Anaphase
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-380284 Loss of proteins required for interphase microtubule organizationfrom the centrosome
R-HSA-2995410 Nuclear Envelope Reassembly
R-HSA-68877 Mitotic Prometaphase
R-HSA-453279 Mitotic G1-G1/S phases
R-HSA-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
R-HSA-69273 Cyclin A/B1 associated events during G2/M transition
R-HSA-2465910 MASTL Facilitates Mitotic Progression
R-HSA-68882 Mitotic Anaphase
R-HSA-69206 G1/S Transition
R-HSA-2995383 Initiation of Nuclear Envelope Reformation
R-HSA-2565942 Regulation of PLK1 Activity at G2/M Transition
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-71387 Metabolism of carbohydrates
R-HSA-1430728 Metabolism
R-HSA-70326 Glucose metabolism
R-HSA-70171 Glycolysis
R-HSA-163685 Integration of energy metabolism
R-HSA-163767 PP2A-mediated dephosphorylation of key metabolic factors
R-HSA-1266738 Developmental Biology
R-HSA-375165 NCAM signaling for neurite out-growth
R-HSA-422475 Axon guidance
Pathway Interaction Database
plk1_pathway PLK1 signaling events
wnt_canonical_pathway Canonical Wnt signaling pathway
erbb1_downstream_pathway ErbB1 downstream signaling
WikiPathways
WP399 Wnt Signaling Pathway and Pluripotency
WP500 Glycogen Metabolism
WP364 IL-6 Signaling Pathway


Homo sapiens (human) PPP2R1A NP_055040.2
Pan troglodytes (chimpanzee) PPP2R1A XP_001174546.1
Macaca mulatta (Rhesus monkey) PPP2R1A XP_001116604.1
Canis lupus familiaris (dog) LOC484335 XP_850993.1
Bos taurus (cattle) PPP2R1A NP_001032554.1
Mus musculus (house mouse) Ppp2r1a NP_058587.1
Rattus norvegicus (Norway rat) Ppp2r1a NP_476481.1
Drosophila melanogaster (fruit fly) Pp2A-29B NP_001027225.1
Caenorhabditis elegans paa-1 NP_498162.2
Arabidopsis thaliana (thale cress) RCN1 NP_173920.1
Xenopus (Silurana) tropicalis (western clawed frog) ppp2r1a NP_989405.1

Component

ID Name Evidence
GO:0000159 protein phosphatase type 2A complex IDA
GO:0000159 protein phosphatase type 2A complex TAS
GO:0000775 chromosome, centromeric region IDA
GO:0005625 soluble fraction NAS
GO:0005634 nucleus NAS
GO:0005694 chromosome IEA
GO:0005737 cytoplasm IEA
GO:0005739 mitochondrion NAS
GO:0005829 cytosol EXP
GO:0005829 cytosol TAS
GO:0015630 microtubule cytoskeleton NAS
GO:0016020 membrane NAS

Function

ID Name Evidence
GO:0003823 antigen binding IPI
GO:0005515 protein binding IPI
GO:0008601 protein phosphatase type 2A regulator activity TAS
GO:0046982 protein heterodimerization activity IPI

Process

ID Name Evidence
GO:0000086 G2/M transition of mitotic cell cycle TAS
GO:0000188 inactivation of MAPK activity NAS
GO:0000278 mitotic cell cycle TAS
GO:0006275 regulation of DNA replication NAS
GO:0006355 regulation of transcription, DNA-dependent NAS
GO:0006461 protein complex assembly TAS
GO:0006470 protein dephosphorylation TAS
GO:0006672 ceramide metabolic process NAS
GO:0006917 induction of apoptosis TAS
GO:0007059 chromosome segregation IDA
GO:0008380 RNA splicing NAS
GO:0010033 response to organic substance NAS
GO:0019932 second-messenger-mediated signaling NAS
GO:0030111 regulation of Wnt receptor signaling pathway NAS
GO:0030155 regulation of cell adhesion NAS
GO:0030308 negative regulation of cell growth NAS
GO:0040008 regulation of growth NAS
GO:0042518 negative regulation of tyrosine phosphorylation of Stat3 protein NAS
GO:0045595 regulation of cell differentiation NAS

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PPP2R1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP2R1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu32300
NM_014225 Homo sapiens protein phosphatase 2, regulatory subunit A, alpha (PPP2R1A), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.30
$99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu32300
Accession Version NM_014225.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1770bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-MAY-2015
Organism Homo sapiens (human)
Product serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC398468.1, BC026733.1 and CA426613.1. On Apr 16, 2010 this sequence version replaced gi:206725413. Summary: This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290593.1, BC026733.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCGGCGG CCGACGGCGA CGACTCGCTG TACCCCATCG CGGTGCTCAT AGACGAACTC 
CGCAATGAGG ACGTTCAGCT TCGCCTCAAC AGCATCAAGA AGCTGTCCAC CATCGCCTTG
GCCCTTGGGG TTGAAAGGAC CCGAAGTGAG CTTCTGCCTT TCCTTACAGA TACCATCTAT
GATGAAGATG AGGTCCTCCT GGCCCTGGCA GAACAGCTGG GAACCTTCAC TACCCTGGTG
GGAGGCCCAG AGTACGTGCA CTGCCTGCTG CCACCGCTGG AGTCGCTGGC CACAGTGGAG
GAGACAGTGG TGCGGGACAA GGCAGTGGAG TCCTTACGGG CCATCTCACA CGAGCACTCG
CCCTCTGACC TGGAGGCGCA CTTTGTGCCG CTAGTGAAGC GGCTGGCGGG CGGCGACTGG
TTCACCTCCC GCACCTCGGC CTGCGGCCTC TTCTCCGTCT GCTACCCCCG AGTGTCCAGT
GCTGTGAAGG CGGAACTTCG ACAGTACTTC CGGAACCTGT GCTCAGATGA CACCCCCATG
GTGCGGCGGG CCGCAGCCTC CAAGCTGGGG GAGTTTGCCA AGGTGCTGGA GCTGGACAAC
GTCAAGAGTG AGATCATCCC CATGTTCTCC AACCTGGCCT CTGACGAGCA GGACTCGGTG
CGGCTGCTGG CGGTGGAGGC GTGCGTGAAC ATCGCCCAGC TTCTGCCCCA GGAGGATCTG
GAGGCCCTGG TGATGCCCAC TCTGCGCCAG GCCGCTGAAG ACAAGTCCTG GCGCGTCCGC
TACATGGTGG CTGACAAGTT CACAGAGCTC CAGAAAGCAG TGGGGCCTGA GATCACCAAG
ACAGACCTGG TCCCTGCCTT CCAGAACCTG ATGAAAGACT GTGAGGCCGA GGTGAGGGCC
GCAGCCTCCC ACAAGGTCAA AGAGTTCTGT GAAAACCTCT CAGCTGACTG TCGGGAGAAT
GTGATCATGT CCCAGATCTT GCCCTGCATC AAGGAGCTGG TGTCCGATGC CAACCAACAT
GTCAAGTCTG CCCTGGCCTC AGTCATCATG GGTCTCTCTC CCATCTTGGG CAAAGACAAC
ACCATCGAGC ACCTCTTGCC CCTCTTCCTG GCTCAGCTGA AGGATGAGTG CCCTGAGGTA
CGGCTGAACA TCATCTCTAA CCTGGACTGT GTGAACGAGG TGATTGGCAT CCGGCAGCTG
TCCCAGTCCC TGCTCCCTGC CATTGTGGAG CTGGCTGAGG ACGCCAAGTG GCGGGTGCGG
CTGGCCATCA TTGAGTACAT GCCCCTCCTG GCTGGACAGC TGGGAGTGGA GTTCTTTGAT
GAGAAACTTA ACTCCTTGTG CATGGCCTGG CTTGTGGATC ATGTATATGC CATCCGCGAG
GCAGCCACCA GCAACCTGAA GAAGCTAGTG GAAAAGTTTG GGAAGGAGTG GGCCCATGCC
ACAATCATCC CCAAGGTCTT GGCCATGTCC GGAGACCCCA ACTACCTGCA CCGCATGACT
ACGCTCTTCT GCATCAATGT GCTGTCTGAG GTCTGTGGGC AGGACATCAC CACCAAGCAC
ATGCTACCCA CGGTTCTGCG CATGGCTGGG GACCCGGTTG CCAATGTCCG CTTCAATGTG
GCCAAGTCTC TGCAGAAGAT AGGGCCCATC CTGGACAACA GCACCTTGCA GAGTGAAGTC
AAGCCCATCC TAGAGAAGCT GACCCAGGAC CAGGATGTGG ACGTCAAATA CTTTGCCCAG
GAGGCTCTGA CTGTTCTGTC TCTCGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_055040.2
CDS296..2065
Misc Feature(1)272..274(+)
Misc Feature(2)299..301(+)
Misc Feature(3)317..1492(+)
Misc Feature(4)434..1258(+)
Misc Feature(5)548..1012(+)
Misc Feature(6)830..988(+)
Misc Feature(7)854..>1681(+)
Misc Feature(8)1133..1135(+)
Misc Feature(9)1481..>1657(+)
Misc Feature(10)1493..2062(+)
Misc Feature(11)1757..2044(+)
Exon (1)1..373
Gene:PPP2R1A
Gene Synonym:
Exon (2)374..464
Gene:PPP2R1A
Gene Synonym:
Exon (3)465..565
Gene:PPP2R1A
Gene Synonym:
Exon (4)566..798
Gene:PPP2R1A
Gene Synonym:
Exon (5)799..946
Gene:PPP2R1A
Gene Synonym:
Exon (6)947..1102
Gene:PPP2R1A
Gene Synonym:
Exon (7)1103..1217
Gene:PPP2R1A
Gene Synonym:
Exon (8)1218..1288
Gene:PPP2R1A
Gene Synonym:
Exon (9)1289..1423
Gene:PPP2R1A
Gene Synonym:
Exon (10)1424..1597
Gene:PPP2R1A
Gene Synonym:
Exon (11)1598..1658
Gene:PPP2R1A
Gene Synonym:
Exon (12)1659..1813
Gene:PPP2R1A
Gene Synonym:
Exon (13)1814..1956
Gene:PPP2R1A
Gene Synonym:
Exon (14)1957..2048
Gene:PPP2R1A
Gene Synonym:
Exon (15)2049..2509
Gene:PPP2R1A
Gene Synonym:
Translation
Position Chain Variation Link
23 23 c, g dbSNP:112009029
31 31 c, t dbSNP:530932890
57 57 a, t dbSNP:549762963
102 102 a, g dbSNP:374414470
106 106 a, g dbSNP:184163465
120 120 c, t dbSNP:561277255
122 122 g, t dbSNP:528535682
123 123 g, t dbSNP:547310883
124 124 a, c dbSNP:571874809
126 126 -, a dbSNP:34945683
130 130 g, t dbSNP:764923113
150 150 c, g, t dbSNP:112621280
218 218 c, t dbSNP:550908149
223 223 c, t dbSNP:13344984
235 235 -, c, cc dbSNP:761513244
236 236 -, c dbSNP:67495004
236 236 c, t dbSNP:771857960
239 239 -, tc dbSNP:112482978
239 239 a, c dbSNP:535966011
240 240 -, a dbSNP:200079168
243 243 -, t dbSNP:201332370
243 243 c, g, t dbSNP:111861947
244 244 a, cg dbSNP:3833207
244 244 a, c, g, t dbSNP:17554825
245 245 a, c dbSNP:62109200
246 246 a, g dbSNP:200899936
258 258 c, t dbSNP:779419476
267 267 c, g dbSNP:750734856
276 276 a, g dbSNP:199921290
295 295 a, g dbSNP:780026299
307 307 c, t dbSNP:140334526
331 331 c, t dbSNP:768487870
334 334 c, t dbSNP:201882921
338 338 a, g dbSNP:781037034
358 358 c, t dbSNP:748072590
364 364 a, g dbSNP:770677329
370 370 g, t dbSNP:587778622
409 409 c, t dbSNP:775159886
425 425 a, g dbSNP:746459436
434 434 -, c dbSNP:758577364
438 438 a, g dbSNP:770424653
448 448 g, t dbSNP:773832415
451 451 g, t dbSNP:763355705
463 463 a, c dbSNP:766816615
487 487 a, g, t dbSNP:11537696
493 493 a, c dbSNP:771300541
500 500 c, t dbSNP:774799372
508 508 a, g dbSNP:759838097
525 525 c, t dbSNP:763692367
526 526 c, t dbSNP:371261424
527 527 a, c dbSNP:559725266
531 531 c, t dbSNP:772287376
532 532 g, t dbSNP:775755972
550 550 c, t dbSNP:760874499
554 554 c, t dbSNP:144146832
568 568 a, g dbSNP:749324986
571 571 a, g dbSNP:765353178
579 579 c, t dbSNP:148604195
580 580 a, g dbSNP:142069409
598 598 a, g dbSNP:766315805
626 626 g, t dbSNP:751360442
632 632 a, c dbSNP:113545781
644 644 c, g dbSNP:767017930
646 646 c, t dbSNP:752257198
654 654 a, c dbSNP:755649324
655 655 a, g dbSNP:11537700
660 660 c, t dbSNP:11537698
667 667 g, t dbSNP:746424804
672 672 c, t dbSNP:527266620
673 673 a, g, t dbSNP:199586303
685 685 a, g dbSNP:768939238
695 695 c, t dbSNP:546812521
702 702 -, g dbSNP:367620507
702 702 c, g dbSNP:776781710
703 703 a, g dbSNP:748386684
706 706 c, g, t dbSNP:571582129
709 709 c, g, t dbSNP:763013618
721 721 c, t dbSNP:774301300
723 723 c, t dbSNP:112759633
727 727 c, t dbSNP:759187339
733 733 a, g dbSNP:767340233
739 739 c, t dbSNP:752352459
750 750 c, t dbSNP:267605629
751 751 c, t dbSNP:139229358
757 757 c, t dbSNP:763637780
761 761 a, c dbSNP:753308060
763 763 c, g dbSNP:758930597
764 764 c, t dbSNP:780485104
765 765 a, c, g dbSNP:111525410
772 772 a, c, t dbSNP:11537695
775 775 c, t dbSNP:113939958
776 776 g, t dbSNP:763936696
777 777 c, t dbSNP:557584612
778 778 c, t dbSNP:770017081
779 779 a, g dbSNP:146859239
786 786 c, t dbSNP:749322788
787 787 a, g dbSNP:200540971
790 790 a, g dbSNP:200123377
831 831 c, t dbSNP:786205228
832 832 c, g, t dbSNP:201816987
839 839 c, t dbSNP:786205227
841 841 a, g dbSNP:745835001
847 847 c, t dbSNP:772009912
862 862 c, g dbSNP:140667985
865 865 a, g dbSNP:760464308
881 881 c, t dbSNP:768304617
886 886 a, g dbSNP:776319121
894 894 a, g dbSNP:761428039
895 895 c, t dbSNP:764764277
896 896 a, g dbSNP:749905469
899 899 a, c dbSNP:759982626
901 901 a, g dbSNP:768008940
902 902 a, t dbSNP:372716916
904 904 c, t dbSNP:756612403
920 920 c, t dbSNP:778270058
927 927 a, g dbSNP:201263415
929 929 c, t dbSNP:757523472
937 937 c, t dbSNP:778892281
940 940 c, t dbSNP:150469675
952 952 a, g dbSNP:750477691
967 967 a, g dbSNP:371185672
972 972 a, g dbSNP:199784281
973 973 a, g dbSNP:11537697
976 976 a, g dbSNP:145417094
979 979 c, t dbSNP:201531933
986 986 a, g dbSNP:780984008
988 988 c, t dbSNP:149134744
989 989 a, g dbSNP:769334090
991 991 c, t dbSNP:772983821
996 996 g, t dbSNP:748878328
998 998 c, t dbSNP:770524297
1012 1012 g, t dbSNP:773968295
1022 1022 c, g dbSNP:761297008
1023 1023 a, t dbSNP:766491619
1036 1036 c, t dbSNP:764648771
1046 1046 g, t dbSNP:777150923
1048 1048 c, t dbSNP:756508136
1054 1054 a, g dbSNP:765519305
1069 1069 c, t dbSNP:750719391
1084 1084 a, g dbSNP:758513581
1097 1097 a, g dbSNP:766508104
1099 1099 a, g dbSNP:751586524
1120 1120 g, t dbSNP:755974835
1121 1121 a, c dbSNP:777655260
1141 1141 c, t dbSNP:11537699
1166 1166 a, t dbSNP:753529647
1183 1183 c, t dbSNP:756949044
1193 1193 g, t dbSNP:531304664
1194 1194 c, t dbSNP:778644015
1195 1195 c, t dbSNP:745513453
1196 1196 a, g dbSNP:771769470
1204 1204 c, t dbSNP:371666912
1213 1213 c, t dbSNP:762227086
1215 1215 a, g dbSNP:376742581
1234 1234 c, t dbSNP:771332782
1239 1239 c, t dbSNP:553406548
1240 1240 c, t dbSNP:746120438
1245 1245 c, g dbSNP:772234016
1247 1247 a, c, t dbSNP:775654171
1247 1247 -, c dbSNP:770783394
1248 1248 a, g dbSNP:760606403
1275 1275 c, t dbSNP:561750024
1285 1285 c, t dbSNP:529026677
1294 1294 c, g dbSNP:780753812
1300 1300 c, t dbSNP:569121300
1331 1331 a, g dbSNP:777641879
1339 1339 c, t dbSNP:773142135
1351 1351 c, t dbSNP:749256759
1381 1381 c, t dbSNP:370489148
1382 1382 a, g dbSNP:751218909
1387 1387 c, t dbSNP:759195182
1388 1388 c, t dbSNP:373680053
1394 1394 c, g, t dbSNP:377119375
1414 1414 c, g dbSNP:757765834
1425 1425 a, g dbSNP:780300274
1457 1457 c, t dbSNP:370377898
1463 1463 c, t dbSNP:755173131
1471 1471 c, t dbSNP:143253734
1495 1495 a, g dbSNP:748379874
1519 1519 c, t dbSNP:770036949
1531 1531 a, t dbSNP:777978846
1537 1537 c, t dbSNP:139139629
1547 1547 c, t dbSNP:770861676
1551 1551 g, t dbSNP:774020151
1553 1553 c, g dbSNP:759316091
1557 1557 -, g dbSNP:138256580
1579 1579 c, t dbSNP:771749848
1600 1600 a, c dbSNP:201487228
1631 1631 c, t dbSNP:569487159
1661 1661 c, t dbSNP:746735616
1666 1666 c, t dbSNP:768431073
1672 1672 c, t dbSNP:776415548
1673 1673 a, g dbSNP:747728763
1684 1684 c, t dbSNP:144544128
1702 1702 a, t dbSNP:550955633
1719 1719 a, g dbSNP:760153767
1722 1722 a, c dbSNP:772640939
1725 1725 g, t dbSNP:776010903
1727 1727 g, t dbSNP:200975975
1731 1731 a, g dbSNP:141690261
1741 1741 c, t dbSNP:754127404
1746 1746 c, g dbSNP:761915943
1765 1765 a, c, t dbSNP:187141962
1767 1767 c, g dbSNP:746181547
1786 1786 c, t dbSNP:750426851
1798 1798 a, g dbSNP:758332210
1799 1799 c, t dbSNP:780028635
1801 1801 c, t dbSNP:751497921
1808 1808 a, g dbSNP:754810094
1810 1810 c, g dbSNP:780829641
1812 1812 a, g dbSNP:747860320
1819 1819 a, g dbSNP:80165164
1825 1825 a, g dbSNP:777348773
1828 1828 c, g dbSNP:748943984
1832 1832 a, g dbSNP:770604606
1842 1842 c, t dbSNP:778543586
1866 1866 c, t dbSNP:747537922
1867 1867 a, g, t dbSNP:147064842
1873 1873 a, g dbSNP:748399975
1874 1874 c, t dbSNP:770194852
1875 1875 a, g, t dbSNP:758740333
1876 1876 c, t dbSNP:551593407
1883 1883 a, g dbSNP:766471656
1891 1891 a, g dbSNP:141304480
1903 1903 c, t dbSNP:759524433
1904 1904 c, t dbSNP:767299944
1912 1912 c, t dbSNP:752667767
1937 1937 a, g dbSNP:376352060
1962 1962 g, t dbSNP:759592470
1966 1966 c, g dbSNP:767632211
1976 1976 a, c dbSNP:368375470
1977 1977 a, t dbSNP:760638097
1979 1979 a, c dbSNP:199937493
1987 1987 a, g dbSNP:138066943
1994 1994 c, t dbSNP:756971999
1999 1999 a, c dbSNP:764920637
2001 2001 a, g dbSNP:750082862
2002 2002 g, t dbSNP:757889759
2007 2007 a, c dbSNP:779598008
2013 2013 c, t dbSNP:748626983
2015 2015 a, g dbSNP:756606764
2017 2017 c, t dbSNP:10413435
2021 2021 a, g dbSNP:749597917
2060 2060 a, g, t dbSNP:780001633
2068 2068 c, t dbSNP:768839698
2071 2071 c, g dbSNP:776772246
2082 2082 a, t dbSNP:761824188
2085 2085 a, g dbSNP:769738573
2105 2105 c, g dbSNP:773022362
2114 2114 c, t dbSNP:762520744
2117 2117 c, g dbSNP:766000282
2140 2140 c, t dbSNP:369880957
2150 2150 g, t dbSNP:539393879
2163 2163 c, t dbSNP:551139740
2178 2178 a, g dbSNP:111741898
2195 2195 c, g dbSNP:569551189
2201 2201 c, t dbSNP:41275804
2212 2212 c, t dbSNP:779105435
2267 2267 c, t dbSNP:112316360
2310 2310 c, t dbSNP:137872459
2311 2311 a, c, g dbSNP:113187868
2399 2399 c, t dbSNP:554250483
2400 2400 c, t dbSNP:549791917
2401 2401 a, g dbSNP:546552152
2407 2407 a, c dbSNP:564632679
2416 2416 c, g dbSNP:369518763
2425 2425 g, t dbSNP:80257415
2438 2438 a, g dbSNP:149542408
2447 2447 g, t dbSNP:374801536
2449 2449 a, g dbSNP:561638786
2454 2454 a, t dbSNP:369013078
2463 2463 a, t dbSNP:372430948
2465 2465 a, t dbSNP:781116871

Target ORF information:

RefSeq Version NM_014225
Organism Homo sapiens (human)
Definition Homo sapiens protein phosphatase 2, regulatory subunit A, alpha (PPP2R1A), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_014225

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCGGCGG CCGACGGCGA CGACTCGCTG TACCCCATCG CGGTGCTCAT AGACGAACTC 
CGCAATGAGG ACGTTCAGCT TCGCCTCAAC AGCATCAAGA AGCTGTCCAC CATCGCCTTG
GCCCTTGGGG TTGAAAGGAC CCGAAGTGAG CTTCTGCCTT TCCTTACAGA TACCATCTAT
GATGAAGATG AGGTCCTCCT GGCCCTGGCA GAACAGCTGG GAACCTTCAC TACCCTGGTG
GGAGGCCCAG AGTACGTGCA CTGCCTGCTG CCACCGCTGG AGTCGCTGGC CACAGTGGAG
GAGACAGTGG TGCGGGACAA GGCAGTGGAG TCCTTACGGG CCATCTCACA CGAGCACTCG
CCCTCTGACC TGGAGGCGCA CTTTGTGCCG CTAGTGAAGC GGCTGGCGGG CGGCGACTGG
TTCACCTCCC GCACCTCGGC CTGCGGCCTC TTCTCCGTCT GCTACCCCCG AGTGTCCAGT
GCTGTGAAGG CGGAACTTCG ACAGTACTTC CGGAACCTGT GCTCAGATGA CACCCCCATG
GTGCGGCGGG CCGCAGCCTC CAAGCTGGGG GAGTTTGCCA AGGTGCTGGA GCTGGACAAC
GTCAAGAGTG AGATCATCCC CATGTTCTCC AACCTGGCCT CTGACGAGCA GGACTCGGTG
CGGCTGCTGG CGGTGGAGGC GTGCGTGAAC ATCGCCCAGC TTCTGCCCCA GGAGGATCTG
GAGGCCCTGG TGATGCCCAC TCTGCGCCAG GCCGCTGAAG ACAAGTCCTG GCGCGTCCGC
TACATGGTGG CTGACAAGTT CACAGAGCTC CAGAAAGCAG TGGGGCCTGA GATCACCAAG
ACAGACCTGG TCCCTGCCTT CCAGAACCTG ATGAAAGACT GTGAGGCCGA GGTGAGGGCC
GCAGCCTCCC ACAAGGTCAA AGAGTTCTGT GAAAACCTCT CAGCTGACTG TCGGGAGAAT
GTGATCATGT CCCAGATCTT GCCCTGCATC AAGGAGCTGG TGTCCGATGC CAACCAACAT
GTCAAGTCTG CCCTGGCCTC AGTCATCATG GGTCTCTCTC CCATCTTGGG CAAAGACAAC
ACCATCGAGC ACCTCTTGCC CCTCTTCCTG GCTCAGCTGA AGGATGAGTG CCCTGAGGTA
CGGCTGAACA TCATCTCTAA CCTGGACTGT GTGAACGAGG TGATTGGCAT CCGGCAGCTG
TCCCAGTCCC TGCTCCCTGC CATTGTGGAG CTGGCTGAGG ACGCCAAGTG GCGGGTGCGG
CTGGCCATCA TTGAGTACAT GCCCCTCCTG GCTGGACAGC TGGGAGTGGA GTTCTTTGAT
GAGAAACTTA ACTCCTTGTG CATGGCCTGG CTTGTGGATC ATGTATATGC CATCCGCGAG
GCAGCCACCA GCAACCTGAA GAAGCTAGTG GAAAAGTTTG GGAAGGAGTG GGCCCATGCC
ACAATCATCC CCAAGGTCTT GGCCATGTCC GGAGACCCCA ACTACCTGCA CCGCATGACT
ACGCTCTTCT GCATCAATGT GCTGTCTGAG GTCTGTGGGC AGGACATCAC CACCAAGCAC
ATGCTACCCA CGGTTCTGCG CATGGCTGGG GACCCGGTTG CCAATGTCCG CTTCAATGTG
GCCAAGTCTC TGCAGAAGAT AGGGCCCATC CTGGACAACA GCACCTTGCA GAGTGAAGTC
AAGCCCATCC TAGAGAAGCT GACCCAGGAC CAGGATGTGG ACGTCAAATA CTTTGCCCAG
GAGGCTCTGA CTGTTCTGTC TCTCGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

PR65A phosphorylation regulates PP2A complex signaling
PLoS ONE 9 (1), E85000 (2014)
Kotlo K, Xing Y, Lather S, Grillon JM, Johnson K, Skidgel RA, Solaro RJ and Danziger RS.


book

Intermittent hypoxia-induced protein phosphatase 2A activation reduces PC12 cell proliferation and differentiation
J. Biomed. Sci. 21, 46 (2014)
Chen TI, Chiu HW, Pan YC, Hsu ST, Lin JH and Yang KT.


book

Complex energy landscape of a giant repeat protein
Structure 21 (11), 1954-1965 (2013)
Tsytlonok M, Craig PO, Sivertsson E, Serquera D, Perrett S, Best RB, Wolynes PG and Itzhaki LS.


book

Functional genetic polymorphisms in PP2A subunit genes confer increased risks of lung cancer in southern and eastern Chinese
PLoS ONE 8 (10), E77285 (2013)
Yang R, Yang L, Qiu F, Zhang L, Wang H, Yang X, Deng J, Fang W, Zhou Y and Lu J.


book

Infrequent mutations of the PPP2R1A and PPP2R1B genes in patients with ovarian cancer
Mol Med Rep 7 (6), 1826-1830 (2013)
Wang F, Zou Y, Liu FY, Yu XH, Huang H, Zhang N, Qi YY, Liu RF, Liu XY, Chen J, Huang OP and He M.


book

Identification of a new family of protein phosphatase 2A regulatory subunits
J. Biol. Chem. 270 (44), 26123-26128 (1995)
McCright B and Virshup DM.


book

Cell cycle regulation of the activity and subcellular localization of Plk1, a human protein kinase implicated in mitotic spindle function
J. Cell Biol. 129 (6), 1617-1628 (1995)
Golsteyn RM, Mundt KE, Fry AM and Nigg EA.


book

alpha- and beta-forms of the 65-kDa subunit of protein phosphatase 2A have a similar 39 amino acid repeating structure
Biochemistry 29 (13), 3166-3173 (1990)
Hemmings BA, Adams-Pearson C, Maurer F, Muller P, Goris J, Merlevede W, Hofsteenge J and Stone SR.


book

Association of protein phosphatase 2A with polyoma virus medium tumor antigen
Proc. Natl. Acad. Sci. U.S.A. 87 (7), 2521-2525 (1990)
Walter G, Ruediger R, Slaughter C and Mumby M.


book

Molecular cloning and sequence of cDNA encoding polyoma medium tumor antigen-associated 61-kDa protein
Proc. Natl. Acad. Sci. U.S.A. 86 (22), 8669-8672 (1989)
Walter G, Ferre F, Espiritu O and Carbone-Wiley A.


 
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