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ETNK2 ethanolamine kinase 2 [Homo sapiens (human)]

Gene Symbol ETNK2
Entrez Gene ID 55224
Full Name ethanolamine kinase 2
Synonyms EKI2, HMFT1716
General protein information
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q32.1

Summary The protein encoded by this gene is a member of choline/ethanolamine kinase family which catalyzes the first step of phosphatidylethanolamine (PtdEtn) biosynthesis via the cytidine diphosphate (CDP) ethanolamine pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]. lac of sum
Disorder MIM:

609859

Disorder Html:





The following ETNK2 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ETNK2 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu34523 XM_005245303 PREDICTED: Homo sapiens ethanolamine kinase 2 (ETNK2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $319
OHu69190 XM_011509714 PREDICTED: Homo sapiens ethanolamine kinase 2 (ETNK2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu69190 XM_011509715 PREDICTED: Homo sapiens ethanolamine kinase 2 (ETNK2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu13684 NM_018208 Homo sapiens ethanolamine kinase 2 (ETNK2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$265.3
$379
OHu34522 NM_001297760 Homo sapiens ethanolamine kinase 2 (ETNK2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $319
OHu69191 NM_001297761 Homo sapiens ethanolamine kinase 2 (ETNK2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $199
OHu34524 NM_001297762 Homo sapiens ethanolamine kinase 2 (ETNK2), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $319

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu34523
Accession Version XM_005245303.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1062bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ethanolamine kinase 2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTGTGC CCCCTTCGGC CCCTCAGCCG CGCGCGTCCT TTCACCTGAG GAGGCACACG 
CCTTGCCCGC AGTGCTCATG GGGCATGGAG GAGAAGGCGG CGGCCAGCGC CAGCTGCCGG
GAGCCGCCGG GCCCCCCGAG GGCCGCCGCC GTCGCGTACT TCGGCATTTC CGTGGACCCG
GACGACATCC TTCCCGGGGC CCTGCGCCTC ATCCAGGAGC TGCGGCCGCA TTGGAAACCC
GAGCAAGTTC GGACCAAGCG CTTCACGGAT GGCATCACCA ACAAGCTGGT GGCCTGCTAT
GTGGAGGAGG ACATGCAGGA CTGCGTGCTG GTCCGGGTGT ATGGGGAGCG GACGGAGCTG
CTGGTGGACC GGGAGAATGA GGTCAGAAAC TTCCAGCTGC TGCGAGCACA CAGCTGTGCC
CCCAAACTCT ACTGCACCTT CCAGAATGGG CTGTGCTATG AGTACATGCA GGGTGTGGCC
CTGGAGCCTG AGCACATCCG TGAGCCCCGG CTTTTCAGCC TTTCTGCAGA TGTCCCTAAG
GTAGAGGTGT TGGAACGGGA GCTGGCCTGG CTGAAGGAGC ATCTGTCCCA GCTGGAGTCC
CCTGTGGTGT TTTGTCACAA TGACCTGCTC TGCAAGAATA TCATCTATGA CAGCATCAAA
GGTCACGTGC GGTTCATTGA CTATGAATAT GCTGGCTACA ACTACCAAGC TTTTGACATT
GGCAACCATT TCAATGAGTT TGCAGGCGTG AATGAGGTGG ATTACTGCCT GTACCCGGCG
CGGGAGACCC AGCTGCAGTG GCTGCACTAC TACCTGCAGG CACAAAAGGG GATGGCCGTG
ACCCCCAGGG AGGTGCAAAG GCTCTACGTG CAAGTCAACA AGTTTGCCCT GGGTCCTAGC
TGTGTGTCTT CCACAATGAC TGCATCCCTC CAGTGCTGTA GAGTCGGAAA CAGGCATGGG
GAGATTGCCA GGCTGACCCT CTCTGGTCTG TTTCCAGGCG TCTCACTTCT TCTGGGCTCT
CTGGGCCCTC ATCCAGAACC AGTACTCCAC CATCGACTTT GA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005245360.1
CDS129..1190
Misc Feature(1)375..1019(+)
Misc Feature(2)393..881(+)
Misc Feature(3)393..809(+)
Misc Feature(4)399..881(+)
Misc Feature(5)657..1019(+)
Translation
Position Chain Variation Link
10 10 a, g dbSNP:550740913
40 40 c, g dbSNP:530942837
47 47 c, t dbSNP:12738682
88 88 a, c dbSNP:568533842
95 95 a, c dbSNP:12738530
111 111 g, t dbSNP:548341006
120 120 a, c dbSNP:776557336
128 128 a, g dbSNP:770609376
141 141 c, g dbSNP:746714627
156 156 a, c dbSNP:772697570
157 157 a, c dbSNP:3737655
159 159 c, t dbSNP:747596168
163 163 c, g dbSNP:755121524
174 174 c, t dbSNP:559449870
210 210 a, g dbSNP:540551989
221 221 -, g dbSNP:751841362
264 264 c, t dbSNP:779395017
266 266 a, g dbSNP:755542077
268 268 g, t dbSNP:749741774
273 273 a, g dbSNP:533238188
278 278 c, t dbSNP:756406936
315 315 a, c dbSNP:564304932
343 343 a, g dbSNP:376162037
388 388 a, g dbSNP:374386002
394 394 c, t dbSNP:760338329
409 409 a, g dbSNP:149143962
416 416 a, g dbSNP:771641428
418 418 c, t dbSNP:747789899
426 426 c, t dbSNP:773906422
427 427 a, g dbSNP:768128714
429 429 a, g dbSNP:370680512
432 432 a, g dbSNP:530687373
452 452 c, t dbSNP:200236107
453 453 a, g dbSNP:746164408
461 461 c, g, t dbSNP:757621574
463 463 a, g dbSNP:751819221
467 467 a, g dbSNP:764309147
478 478 a, g dbSNP:202185285
481 481 c, t dbSNP:528212957
482 482 a, g dbSNP:372992385
487 487 c, t dbSNP:370190989
498 498 c, t dbSNP:761624943
499 499 a, c, g dbSNP:201400955
506 506 g, t dbSNP:761499775
515 515 a, g dbSNP:773965589
522 522 c, t dbSNP:768188076
524 524 c, g dbSNP:762324880
525 525 c, g dbSNP:776081427
531 531 a, c dbSNP:770133377
544 544 a, g dbSNP:745615577
551 551 c, t dbSNP:781729192
558 558 c, t dbSNP:559020342
563 563 c, t dbSNP:370194168
586 586 a, g dbSNP:377403947
590 590 a, g dbSNP:758518203
594 594 a, c, t dbSNP:779042118
599 599 a, g dbSNP:373432247
602 602 c, t dbSNP:200166795
618 618 a, g dbSNP:750335710
623 623 c, t dbSNP:576441238
627 627 c, t dbSNP:141281450
628 628 a, g dbSNP:144504466
635 635 c, t dbSNP:763600102
636 636 c, t dbSNP:762532276
637 637 a, g dbSNP:774930573
655 655 c, t dbSNP:745574189
658 658 a, g dbSNP:373228420
663 663 a, c dbSNP:770497691
669 669 a, g dbSNP:746465912
670 670 c, t dbSNP:777258273
676 676 c, t dbSNP:758961107
677 677 a, g dbSNP:748630621
678 678 c, t dbSNP:779374402
681 681 a, g dbSNP:755254782
684 684 c, t dbSNP:754108955
685 685 a, g, t dbSNP:3737657
686 686 a, g dbSNP:138409813
688 688 -, a dbSNP:35235705
689 689 a, g dbSNP:767695300
700 700 c, t dbSNP:763037814
707 707 c, g dbSNP:775542456
713 713 g, t dbSNP:759163609
720 720 a, c dbSNP:200858943
728 728 c, g dbSNP:776320731
731 731 g, t dbSNP:116285702
740 740 c, t dbSNP:770425571
747 747 a, g dbSNP:746649734
753 753 c, t dbSNP:772756403
773 773 c, t dbSNP:771539071
776 776 c, t dbSNP:748681339
794 794 c, t dbSNP:140913993
795 795 a, g dbSNP:374969247
797 797 a, g dbSNP:771348515
798 798 c, t dbSNP:565275436
799 799 a, g, t dbSNP:554543246
808 808 a, t dbSNP:749201140
809 809 c, t dbSNP:779908230
821 821 c, t dbSNP:200739096
836 836 a, g dbSNP:750284336
846 846 a, g dbSNP:767284095
847 847 c, t dbSNP:756911069
851 851 a, c dbSNP:751194146
862 862 a, g dbSNP:763544137
875 875 c, t dbSNP:752325302
876 876 a, g dbSNP:533254441
881 881 c, t dbSNP:201036824
884 884 a, g dbSNP:777234659
887 887 a, g dbSNP:375907212
898 898 g, t dbSNP:761038426
900 900 c, t dbSNP:750538219
904 904 a, c, t dbSNP:761926179
905 905 a, g, t dbSNP:372782006
906 906 c, g dbSNP:745838701
907 907 a, c, t dbSNP:570954657
908 908 a, g dbSNP:761470297
909 909 -, c dbSNP:770142507
909 909 c, g, t dbSNP:201980742
910 910 a, g dbSNP:200305922
911 911 -, cgcgg dbSNP:748320572
912 912 a, g dbSNP:778594983
914 914 c, g dbSNP:140658518
916 916 c, g dbSNP:754406318
917 917 c, t dbSNP:766839183
918 918 c, t dbSNP:761081739
919 919 a, c dbSNP:750856269
925 925 a, c dbSNP:767749304
933 933 c, g dbSNP:762064107
934 934 a, g dbSNP:764023394
935 935 c, g dbSNP:61825753
937 937 a, g dbSNP:542121001
947 947 a, g dbSNP:776441116
956 956 a, g dbSNP:770971979
957 957 a, g dbSNP:746909963
959 959 a, c, g dbSNP:775624367
960 960 a, t dbSNP:144439568
962 962 a, g dbSNP:747823343
974 974 c, t dbSNP:778646158
983 983 a, g dbSNP:754536920
986 986 a, g dbSNP:149960550
992 992 c, t dbSNP:753465248
995 995 c, t dbSNP:146679279
996 996 a, g dbSNP:756691101
998 998 a, g dbSNP:750862892
1005 1005 a, g dbSNP:767957800
1011 1011 g, t dbSNP:762117921
1023 1023 c, t dbSNP:764433504
1025 1025 c, t dbSNP:758671106
1030 1030 a, g dbSNP:777569920
1034 1034 a, g dbSNP:752834882
1045 1045 c, t dbSNP:765377969
1048 1048 c, t dbSNP:114520188
1060 1060 a, c dbSNP:773202231
1070 1070 a, g dbSNP:767625923
1071 1071 a, g dbSNP:761828449
1073 1073 c, t dbSNP:538887924
1074 1074 a, g dbSNP:374814783
1091 1091 c, g dbSNP:34175169
1104 1104 a, g dbSNP:111991093
1110 1110 a, t dbSNP:774957303
1127 1127 c, t dbSNP:769362056
1128 1128 a, g, t dbSNP:780677236
1136 1136 a, t dbSNP:370655182
1168 1168 c, g dbSNP:747496173
1177 1177 a, c dbSNP:778153843
1182 1182 a, c, t dbSNP:752974385
1183 1183 a, g dbSNP:148772808
1196 1196 a, t dbSNP:755219297
1203 1203 c, t dbSNP:76205451
1205 1205 c, t dbSNP:780309918
1213 1213 c, t dbSNP:756213388
1218 1218 c, g dbSNP:746568489
1242 1242 c, t dbSNP:751557066
1248 1248 a, g dbSNP:764058133
1253 1253 c, t dbSNP:762943597
1260 1260 a, g dbSNP:752493579
1271 1271 a, g dbSNP:371581071
1286 1286 c, t dbSNP:759156287
1292 1292 c, g, t dbSNP:574964789
1293 1293 -, c dbSNP:780402256
1296 1296 c, t dbSNP:368115670
1300 1300 c, t dbSNP:539891992
1306 1306 g, t dbSNP:772509364
1309 1309 a, c dbSNP:748662740
1314 1314 c, g dbSNP:779185057
1340 1340 c, t dbSNP:535590418
1415 1415 a, g dbSNP:775177450
1421 1421 c, g dbSNP:566547978
1429 1429 a, g dbSNP:139047077
1446 1446 c, t dbSNP:539339572
1460 1460 a, g dbSNP:777186551
1470 1470 c, t dbSNP:151131812
1483 1483 c, t dbSNP:763143837
1544 1544 c, t dbSNP:41313900
1548 1548 c, t dbSNP:139891629
1564 1564 a, t dbSNP:759820778
1566 1566 c, t dbSNP:117726554
1612 1612 a, g dbSNP:771663723
1634 1634 a, g dbSNP:77936713
1637 1637 a, c dbSNP:75876713
1638 1638 a, t dbSNP:79403699
1668 1668 a, g dbSNP:549500658
1688 1688 c, g dbSNP:746575421
1749 1749 c, g dbSNP:777135117
1780 1780 g, t dbSNP:529432849
1785 1785 a, g dbSNP:777254623
1792 1792 c, g dbSNP:181336042
1801 1801 a, g dbSNP:771583361
1811 1811 c, g dbSNP:746474734
1835 1835 c, t dbSNP:772816567
1876 1876 c, t dbSNP:76718240
1877 1877 a, g dbSNP:533436486
1896 1896 c, t dbSNP:564460294
1922 1922 c, t dbSNP:543769555
1931 1931 c, g dbSNP:574897142
1983 1983 a, g dbSNP:554992422
2001 2001 g, t dbSNP:541550800
2004 2004 a, g dbSNP:145968121
2018 2018 c, t dbSNP:778495752
2102 2102 c, t dbSNP:754746019
2135 2135 c, t dbSNP:114187399
2193 2193 -, c dbSNP:375255895
2255 2255 c, g dbSNP:539644532
2264 2264 c, g, t dbSNP:557304214
2311 2311 a, g dbSNP:116590745
2330 2330 c, t dbSNP:569270610
2385 2385 c, t dbSNP:780002989

Target ORF information:

RefSeq Version XM_005245303
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ethanolamine kinase 2 (ETNK2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005245303

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTGTGC CCCCTTCGGC CCCTCAGCCG CGCGCGTCCT TTCACCTGAG GAGGCACACG 
CCTTGCCCGC AGTGCTCATG GGGCATGGAG GAGAAGGCGG CGGCCAGCGC CAGCTGCCGG
GAGCCGCCGG GCCCCCCGAG GGCCGCCGCC GTCGCGTACT TCGGCATTTC CGTGGACCCG
GACGACATCC TTCCCGGGGC CCTGCGCCTC ATCCAGGAGC TGCGGCCGCA TTGGAAACCC
GAGCAAGTTC GGACCAAGCG CTTCACGGAT GGCATCACCA ACAAGCTGGT GGCCTGCTAT
GTGGAGGAGG ACATGCAGGA CTGCGTGCTG GTCCGGGTGT ATGGGGAGCG GACGGAGCTG
CTGGTGGACC GGGAGAATGA GGTCAGAAAC TTCCAGCTGC TGCGAGCACA CAGCTGTGCC
CCCAAACTCT ACTGCACCTT CCAGAATGGG CTGTGCTATG AGTACATGCA GGGTGTGGCC
CTGGAGCCTG AGCACATCCG TGAGCCCCGG CTTTTCAGCC TTTCTGCAGA TGTCCCTAAG
GTAGAGGTGT TGGAACGGGA GCTGGCCTGG CTGAAGGAGC ATCTGTCCCA GCTGGAGTCC
CCTGTGGTGT TTTGTCACAA TGACCTGCTC TGCAAGAATA TCATCTATGA CAGCATCAAA
GGTCACGTGC GGTTCATTGA CTATGAATAT GCTGGCTACA ACTACCAAGC TTTTGACATT
GGCAACCATT TCAATGAGTT TGCAGGCGTG AATGAGGTGG ATTACTGCCT GTACCCGGCG
CGGGAGACCC AGCTGCAGTG GCTGCACTAC TACCTGCAGG CACAAAAGGG GATGGCCGTG
ACCCCCAGGG AGGTGCAAAG GCTCTACGTG CAAGTCAACA AGTTTGCCCT GGGTCCTAGC
TGTGTGTCTT CCACAATGAC TGCATCCCTC CAGTGCTGTA GAGTCGGAAA CAGGCATGGG
GAGATTGCCA GGCTGACCCT CTCTGGTCTG TTTCCAGGCG TCTCACTTCT TCTGGGCTCT
CTGGGCCCTC ATCCAGAACC AGTACTCCAC CATCGACTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu69190
Accession Version XM_011509714.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 651bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ethanolamine kinase 2 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGGCAAAGA TTCATACTAT CCACGCCAAC GGCAGCCTGC CCAAGCCCAT CCTCTGGCAC 
AAGATGCACA ATTATTTCAC GCTTGTGAAG AACGAGATCA ACCCCAGCCT TTCTGCAGAT
GTCCCTAAGG TAGAGGTGTT GGAACGGGAG CTGGCCTGGC TGAAGGAGCA TCTGTCCCAG
CTGGAGTCCC CTGTGGTGTT TTGTCACAAT GACCTGCTCT GCAAGAATAT CATCTATGAC
AGCATCAAAG GTCACGTGCG GTTCATTGAC TATGAATATG CTGGCTACAA CTACCAAGCT
TTTGACATTG GCAACCATTT CAATGAGTTT GCAGGCGTGA ATGAGGTGGA TTACTGCCTG
TACCCGGCGC GGGAGACCCA GCTGCAGTGG CTGCACTACT ACCTGCAGGC ACAAAAGGGG
ATGGCCGTGA CCCCCAGGGA GGTGCAAAGG CTCTACGTGC AAGTCAACAA GTTTGCCCTG
GGTCCTAGCT GTGTGTCTTC CACAATGACT GCATCCCTCC AGTGCTGTAG AGTCGGAAAC
AGGCATGGGG AGATTGCCAG GCTGACCCTC TCTGGTCTGT TTCCAGGCGT CTCACTTCTT
CTGGGCTCTC TGGGCCCTCA TCCAGAACCA GTACTCCACC ATCGACTTTG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011508016.1
CDS101..751
Misc Feature(1)101..580(+)
Translation
Position Chain Variation Link
18 18 c, t dbSNP:769842555
31 31 c, t dbSNP:148669211
35 35 a, g dbSNP:777728553
49 49 a, t dbSNP:758139182
57 57 c, t dbSNP:747909621
59 59 a, g dbSNP:778567351
65 65 a, t dbSNP:754735254
89 89 a, g dbSNP:778813214
91 91 c, t dbSNP:768537260
92 92 a, g dbSNP:748976903
104 104 a, g dbSNP:370629139
114 114 a, c dbSNP:755701277
115 115 c, t dbSNP:749884971
122 122 c, t dbSNP:780567647
124 124 c, t dbSNP:139650333
125 125 a, g dbSNP:751988639
130 130 c, g dbSNP:1880534
130 130 c, t dbSNP:764482873
131 131 g, t dbSNP:763265470
163 163 c, g dbSNP:752940406
169 169 c, t dbSNP:765374787
180 180 c, t dbSNP:759671962
181 181 a, g, t dbSNP:758154824
193 193 c, t dbSNP:143999368
194 194 a, c, g dbSNP:768590671
199 199 c, t dbSNP:549075990
216 216 c, t dbSNP:745574189
219 219 a, g dbSNP:373228420
224 224 a, c dbSNP:770497691
230 230 a, g dbSNP:746465912
231 231 c, t dbSNP:777258273
237 237 c, t dbSNP:758961107
238 238 a, g dbSNP:748630621
239 239 c, t dbSNP:779374402
242 242 a, g dbSNP:755254782
245 245 c, t dbSNP:754108955
246 246 a, g, t dbSNP:3737657
247 247 a, g dbSNP:138409813
249 249 -, a dbSNP:35235705
250 250 a, g dbSNP:767695300
261 261 c, t dbSNP:763037814
268 268 c, g dbSNP:775542456
274 274 g, t dbSNP:759163609
281 281 a, c dbSNP:200858943
289 289 c, g dbSNP:776320731
292 292 g, t dbSNP:116285702
301 301 c, t dbSNP:770425571
308 308 a, g dbSNP:746649734
314 314 c, t dbSNP:772756403
334 334 c, t dbSNP:771539071
337 337 c, t dbSNP:748681339
355 355 c, t dbSNP:140913993
356 356 a, g dbSNP:374969247
358 358 a, g dbSNP:771348515
359 359 c, t dbSNP:565275436
360 360 a, g, t dbSNP:554543246
369 369 a, t dbSNP:749201140
370 370 c, t dbSNP:779908230
382 382 c, t dbSNP:200739096
397 397 a, g dbSNP:750284336
407 407 a, g dbSNP:767284095
408 408 c, t dbSNP:756911069
412 412 a, c dbSNP:751194146
423 423 a, g dbSNP:763544137
436 436 c, t dbSNP:752325302
437 437 a, g dbSNP:533254441
442 442 c, t dbSNP:201036824
445 445 a, g dbSNP:777234659
448 448 a, g dbSNP:375907212
459 459 g, t dbSNP:761038426
461 461 c, t dbSNP:750538219
465 465 a, c, t dbSNP:761926179
466 466 a, g, t dbSNP:372782006
467 467 c, g dbSNP:745838701
468 468 a, c, t dbSNP:570954657
469 469 a, g dbSNP:761470297
470 470 -, c dbSNP:770142507
470 470 c, g, t dbSNP:201980742
471 471 a, g dbSNP:200305922
472 472 -, cgcgg dbSNP:748320572
473 473 a, g dbSNP:778594983
475 475 c, g dbSNP:140658518
477 477 c, g dbSNP:754406318
478 478 c, t dbSNP:766839183
479 479 c, t dbSNP:761081739
480 480 a, c dbSNP:750856269
486 486 a, c dbSNP:767749304
494 494 c, g dbSNP:762064107
495 495 a, g dbSNP:764023394
496 496 c, g dbSNP:61825753
498 498 a, g dbSNP:542121001
508 508 a, g dbSNP:776441116
517 517 a, g dbSNP:770971979
518 518 a, g dbSNP:746909963
520 520 a, c, g dbSNP:775624367
521 521 a, t dbSNP:144439568
523 523 a, g dbSNP:747823343
535 535 c, t dbSNP:778646158
544 544 a, g dbSNP:754536920
547 547 a, g dbSNP:149960550
553 553 c, t dbSNP:753465248
556 556 c, t dbSNP:146679279
557 557 a, g dbSNP:756691101
559 559 a, g dbSNP:750862892
566 566 a, g dbSNP:767957800
572 572 g, t dbSNP:762117921
584 584 c, t dbSNP:764433504
586 586 c, t dbSNP:758671106
591 591 a, g dbSNP:777569920
595 595 a, g dbSNP:752834882
606 606 c, t dbSNP:765377969
609 609 c, t dbSNP:114520188
621 621 a, c dbSNP:773202231
631 631 a, g dbSNP:767625923
632 632 a, g dbSNP:761828449
634 634 c, t dbSNP:538887924
635 635 a, g dbSNP:374814783
652 652 c, g dbSNP:34175169
665 665 a, g dbSNP:111991093
671 671 a, t dbSNP:774957303
688 688 c, t dbSNP:769362056
689 689 a, g, t dbSNP:780677236
697 697 a, t dbSNP:370655182
729 729 c, g dbSNP:747496173
738 738 a, c dbSNP:778153843
743 743 a, c, t dbSNP:752974385
744 744 a, g dbSNP:148772808
757 757 a, t dbSNP:755219297
764 764 c, t dbSNP:76205451
766 766 c, t dbSNP:780309918
774 774 c, t dbSNP:756213388
779 779 c, g dbSNP:746568489
803 803 c, t dbSNP:751557066
809 809 a, g dbSNP:764058133
814 814 c, t dbSNP:762943597
821 821 a, g dbSNP:752493579
832 832 a, g dbSNP:371581071
847 847 c, t dbSNP:759156287
853 853 c, g, t dbSNP:574964789
854 854 -, c dbSNP:780402256
857 857 c, t dbSNP:368115670
861 861 c, t dbSNP:539891992
867 867 g, t dbSNP:772509364
870 870 a, c dbSNP:748662740
875 875 c, g dbSNP:779185057
901 901 c, t dbSNP:535590418
976 976 a, g dbSNP:775177450
982 982 c, g dbSNP:566547978
990 990 a, g dbSNP:139047077
1007 1007 c, t dbSNP:539339572
1021 1021 a, g dbSNP:777186551
1031 1031 c, t dbSNP:151131812
1044 1044 c, t dbSNP:763143837
1105 1105 c, t dbSNP:41313900
1109 1109 c, t dbSNP:139891629
1125 1125 a, t dbSNP:759820778
1127 1127 c, t dbSNP:117726554
1173 1173 a, g dbSNP:771663723
1195 1195 a, g dbSNP:77936713
1198 1198 a, c dbSNP:75876713
1199 1199 a, t dbSNP:79403699
1229 1229 a, g dbSNP:549500658
1249 1249 c, g dbSNP:746575421
1310 1310 c, g dbSNP:777135117
1341 1341 g, t dbSNP:529432849
1346 1346 a, g dbSNP:777254623
1353 1353 c, g dbSNP:181336042
1362 1362 a, g dbSNP:771583361
1372 1372 c, g dbSNP:746474734
1396 1396 c, t dbSNP:772816567
1437 1437 c, t dbSNP:76718240
1438 1438 a, g dbSNP:533436486
1457 1457 c, t dbSNP:564460294
1483 1483 c, t dbSNP:543769555
1492 1492 c, g dbSNP:574897142
1544 1544 a, g dbSNP:554992422
1562 1562 g, t dbSNP:541550800
1565 1565 a, g dbSNP:145968121
1579 1579 c, t dbSNP:778495752
1663 1663 c, t dbSNP:754746019
1696 1696 c, t dbSNP:114187399
1754 1754 -, c dbSNP:375255895
1816 1816 c, g dbSNP:539644532
1825 1825 c, g, t dbSNP:557304214
1872 1872 a, g dbSNP:116590745
1891 1891 c, t dbSNP:569270610
1946 1946 c, t dbSNP:780002989

Target ORF information:

RefSeq Version XM_011509714
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ethanolamine kinase 2 (ETNK2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509714

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCAAAGA TTCATACTAT CCACGCCAAC GGCAGCCTGC CCAAGCCCAT CCTCTGGCAC 
AAGATGCACA ATTATTTCAC GCTTGTGAAG AACGAGATCA ACCCCAGCCT TTCTGCAGAT
GTCCCTAAGG TAGAGGTGTT GGAACGGGAG CTGGCCTGGC TGAAGGAGCA TCTGTCCCAG
CTGGAGTCCC CTGTGGTGTT TTGTCACAAT GACCTGCTCT GCAAGAATAT CATCTATGAC
AGCATCAAAG GTCACGTGCG GTTCATTGAC TATGAATATG CTGGCTACAA CTACCAAGCT
TTTGACATTG GCAACCATTT CAATGAGTTT GCAGGCGTGA ATGAGGTGGA TTACTGCCTG
TACCCGGCGC GGGAGACCCA GCTGCAGTGG CTGCACTACT ACCTGCAGGC ACAAAAGGGG
ATGGCCGTGA CCCCCAGGGA GGTGCAAAGG CTCTACGTGC AAGTCAACAA GTTTGCCCTG
GGTCCTAGCT GTGTGTCTTC CACAATGACT GCATCCCTCC AGTGCTGTAG AGTCGGAAAC
AGGCATGGGG AGATTGCCAG GCTGACCCTC TCTGGTCTGT TTCCAGGCGT CTCACTTCTT
CTGGGCTCTC TGGGCCCTCA TCCAGAACCA GTACTCCACC ATCGACTTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu69190
Accession Version XM_011509715.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 651bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ethanolamine kinase 2 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGGCAAAGA TTCATACTAT CCACGCCAAC GGCAGCCTGC CCAAGCCCAT CCTCTGGCAC 
AAGATGCACA ATTATTTCAC GCTTGTGAAG AACGAGATCA ACCCCAGCCT TTCTGCAGAT
GTCCCTAAGG TAGAGGTGTT GGAACGGGAG CTGGCCTGGC TGAAGGAGCA TCTGTCCCAG
CTGGAGTCCC CTGTGGTGTT TTGTCACAAT GACCTGCTCT GCAAGAATAT CATCTATGAC
AGCATCAAAG GTCACGTGCG GTTCATTGAC TATGAATATG CTGGCTACAA CTACCAAGCT
TTTGACATTG GCAACCATTT CAATGAGTTT GCAGGCGTGA ATGAGGTGGA TTACTGCCTG
TACCCGGCGC GGGAGACCCA GCTGCAGTGG CTGCACTACT ACCTGCAGGC ACAAAAGGGG
ATGGCCGTGA CCCCCAGGGA GGTGCAAAGG CTCTACGTGC AAGTCAACAA GTTTGCCCTG
GGTCCTAGCT GTGTGTCTTC CACAATGACT GCATCCCTCC AGTGCTGTAG AGTCGGAAAC
AGGCATGGGG AGATTGCCAG GCTGACCCTC TCTGGTCTGT TTCCAGGCGT CTCACTTCTT
CTGGGCTCTC TGGGCCCTCA TCCAGAACCA GTACTCCACC ATCGACTTTG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011508017.1
CDS410..1060
Misc Feature(1)410..889(+)
Translation
Position Chain Variation Link
17 17 a, g dbSNP:550740913
47 47 c, g dbSNP:530942837
54 54 c, t dbSNP:12738682
95 95 a, c dbSNP:568533842
102 102 a, c dbSNP:12738530
118 118 g, t dbSNP:548341006
127 127 a, c dbSNP:776557336
135 135 a, g dbSNP:770609376
148 148 c, g dbSNP:746714627
163 163 a, c dbSNP:772697570
164 164 a, c dbSNP:3737655
166 166 c, t dbSNP:747596168
170 170 c, g dbSNP:755121524
181 181 c, t dbSNP:559449870
217 217 a, g dbSNP:540551989
228 228 -, g dbSNP:751841362
271 271 c, t dbSNP:779395017
273 273 a, g dbSNP:755542077
275 275 g, t dbSNP:749741774
280 280 a, g dbSNP:533238188
285 285 c, t dbSNP:756406936
322 322 a, c dbSNP:564304932
350 350 a, g dbSNP:376162037
398 398 a, g dbSNP:778813214
400 400 c, t dbSNP:768537260
401 401 a, g dbSNP:748976903
413 413 a, g dbSNP:370629139
423 423 a, c dbSNP:755701277
424 424 c, t dbSNP:749884971
431 431 c, t dbSNP:780567647
433 433 c, t dbSNP:139650333
434 434 a, g dbSNP:751988639
439 439 c, g dbSNP:1880534
439 439 c, t dbSNP:764482873
440 440 g, t dbSNP:763265470
472 472 c, g dbSNP:752940406
478 478 c, t dbSNP:765374787
489 489 c, t dbSNP:759671962
490 490 a, g, t dbSNP:758154824
502 502 c, t dbSNP:143999368
503 503 a, c, g dbSNP:768590671
508 508 c, t dbSNP:549075990
525 525 c, t dbSNP:745574189
528 528 a, g dbSNP:373228420
533 533 a, c dbSNP:770497691
539 539 a, g dbSNP:746465912
540 540 c, t dbSNP:777258273
546 546 c, t dbSNP:758961107
547 547 a, g dbSNP:748630621
548 548 c, t dbSNP:779374402
551 551 a, g dbSNP:755254782
554 554 c, t dbSNP:754108955
555 555 a, g, t dbSNP:3737657
556 556 a, g dbSNP:138409813
558 558 -, a dbSNP:35235705
559 559 a, g dbSNP:767695300
570 570 c, t dbSNP:763037814
577 577 c, g dbSNP:775542456
583 583 g, t dbSNP:759163609
590 590 a, c dbSNP:200858943
598 598 c, g dbSNP:776320731
601 601 g, t dbSNP:116285702
610 610 c, t dbSNP:770425571
617 617 a, g dbSNP:746649734
623 623 c, t dbSNP:772756403
643 643 c, t dbSNP:771539071
646 646 c, t dbSNP:748681339
664 664 c, t dbSNP:140913993
665 665 a, g dbSNP:374969247
667 667 a, g dbSNP:771348515
668 668 c, t dbSNP:565275436
669 669 a, g, t dbSNP:554543246
678 678 a, t dbSNP:749201140
679 679 c, t dbSNP:779908230
691 691 c, t dbSNP:200739096
706 706 a, g dbSNP:750284336
716 716 a, g dbSNP:767284095
717 717 c, t dbSNP:756911069
721 721 a, c dbSNP:751194146
732 732 a, g dbSNP:763544137
745 745 c, t dbSNP:752325302
746 746 a, g dbSNP:533254441
751 751 c, t dbSNP:201036824
754 754 a, g dbSNP:777234659
757 757 a, g dbSNP:375907212
768 768 g, t dbSNP:761038426
770 770 c, t dbSNP:750538219
774 774 a, c, t dbSNP:761926179
775 775 a, g, t dbSNP:372782006
776 776 c, g dbSNP:745838701
777 777 a, c, t dbSNP:570954657
778 778 a, g dbSNP:761470297
779 779 -, c dbSNP:770142507
779 779 c, g, t dbSNP:201980742
780 780 a, g dbSNP:200305922
781 781 -, cgcgg dbSNP:748320572
782 782 a, g dbSNP:778594983
784 784 c, g dbSNP:140658518
786 786 c, g dbSNP:754406318
787 787 c, t dbSNP:766839183
788 788 c, t dbSNP:761081739
789 789 a, c dbSNP:750856269
795 795 a, c dbSNP:767749304
803 803 c, g dbSNP:762064107
804 804 a, g dbSNP:764023394
805 805 c, g dbSNP:61825753
807 807 a, g dbSNP:542121001
817 817 a, g dbSNP:776441116
826 826 a, g dbSNP:770971979
827 827 a, g dbSNP:746909963
829 829 a, c, g dbSNP:775624367
830 830 a, t dbSNP:144439568
832 832 a, g dbSNP:747823343
844 844 c, t dbSNP:778646158
853 853 a, g dbSNP:754536920
856 856 a, g dbSNP:149960550
862 862 c, t dbSNP:753465248
865 865 c, t dbSNP:146679279
866 866 a, g dbSNP:756691101
868 868 a, g dbSNP:750862892
875 875 a, g dbSNP:767957800
881 881 g, t dbSNP:762117921
893 893 c, t dbSNP:764433504
895 895 c, t dbSNP:758671106
900 900 a, g dbSNP:777569920
904 904 a, g dbSNP:752834882
915 915 c, t dbSNP:765377969
918 918 c, t dbSNP:114520188
930 930 a, c dbSNP:773202231
940 940 a, g dbSNP:767625923
941 941 a, g dbSNP:761828449
943 943 c, t dbSNP:538887924
944 944 a, g dbSNP:374814783
961 961 c, g dbSNP:34175169
974 974 a, g dbSNP:111991093
980 980 a, t dbSNP:774957303
997 997 c, t dbSNP:769362056
998 998 a, g, t dbSNP:780677236
1006 1006 a, t dbSNP:370655182
1038 1038 c, g dbSNP:747496173
1047 1047 a, c dbSNP:778153843
1052 1052 a, c, t dbSNP:752974385
1053 1053 a, g dbSNP:148772808
1066 1066 a, t dbSNP:755219297
1073 1073 c, t dbSNP:76205451
1075 1075 c, t dbSNP:780309918
1083 1083 c, t dbSNP:756213388
1088 1088 c, g dbSNP:746568489
1112 1112 c, t dbSNP:751557066
1118 1118 a, g dbSNP:764058133
1123 1123 c, t dbSNP:762943597
1130 1130 a, g dbSNP:752493579
1141 1141 a, g dbSNP:371581071
1156 1156 c, t dbSNP:759156287
1162 1162 c, g, t dbSNP:574964789
1163 1163 -, c dbSNP:780402256
1166 1166 c, t dbSNP:368115670
1170 1170 c, t dbSNP:539891992
1176 1176 g, t dbSNP:772509364
1179 1179 a, c dbSNP:748662740
1184 1184 c, g dbSNP:779185057
1210 1210 c, t dbSNP:535590418
1285 1285 a, g dbSNP:775177450
1291 1291 c, g dbSNP:566547978
1299 1299 a, g dbSNP:139047077
1316 1316 c, t dbSNP:539339572
1330 1330 a, g dbSNP:777186551
1340 1340 c, t dbSNP:151131812
1353 1353 c, t dbSNP:763143837
1414 1414 c, t dbSNP:41313900
1418 1418 c, t dbSNP:139891629
1434 1434 a, t dbSNP:759820778
1436 1436 c, t dbSNP:117726554
1482 1482 a, g dbSNP:771663723
1504 1504 a, g dbSNP:77936713
1507 1507 a, c dbSNP:75876713
1508 1508 a, t dbSNP:79403699
1538 1538 a, g dbSNP:549500658
1558 1558 c, g dbSNP:746575421
1619 1619 c, g dbSNP:777135117
1650 1650 g, t dbSNP:529432849
1655 1655 a, g dbSNP:777254623
1662 1662 c, g dbSNP:181336042
1671 1671 a, g dbSNP:771583361
1681 1681 c, g dbSNP:746474734
1705 1705 c, t dbSNP:772816567
1746 1746 c, t dbSNP:76718240
1747 1747 a, g dbSNP:533436486
1766 1766 c, t dbSNP:564460294
1792 1792 c, t dbSNP:543769555
1801 1801 c, g dbSNP:574897142
1853 1853 a, g dbSNP:554992422
1871 1871 g, t dbSNP:541550800
1874 1874 a, g dbSNP:145968121
1888 1888 c, t dbSNP:778495752
1972 1972 c, t dbSNP:754746019
2005 2005 c, t dbSNP:114187399
2063 2063 -, c dbSNP:375255895
2125 2125 c, g dbSNP:539644532
2134 2134 c, g, t dbSNP:557304214
2181 2181 a, g dbSNP:116590745
2200 2200 c, t dbSNP:569270610
2255 2255 c, t dbSNP:780002989

Target ORF information:

RefSeq Version XM_011509715
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ethanolamine kinase 2 (ETNK2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509715

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCAAAGA TTCATACTAT CCACGCCAAC GGCAGCCTGC CCAAGCCCAT CCTCTGGCAC 
AAGATGCACA ATTATTTCAC GCTTGTGAAG AACGAGATCA ACCCCAGCCT TTCTGCAGAT
GTCCCTAAGG TAGAGGTGTT GGAACGGGAG CTGGCCTGGC TGAAGGAGCA TCTGTCCCAG
CTGGAGTCCC CTGTGGTGTT TTGTCACAAT GACCTGCTCT GCAAGAATAT CATCTATGAC
AGCATCAAAG GTCACGTGCG GTTCATTGAC TATGAATATG CTGGCTACAA CTACCAAGCT
TTTGACATTG GCAACCATTT CAATGAGTTT GCAGGCGTGA ATGAGGTGGA TTACTGCCTG
TACCCGGCGC GGGAGACCCA GCTGCAGTGG CTGCACTACT ACCTGCAGGC ACAAAAGGGG
ATGGCCGTGA CCCCCAGGGA GGTGCAAAGG CTCTACGTGC AAGTCAACAA GTTTGCCCTG
GGTCCTAGCT GTGTGTCTTC CACAATGACT GCATCCCTCC AGTGCTGTAG AGTCGGAAAC
AGGCATGGGG AGATTGCCAG GCTGACCCTC TCTGGTCTGT TTCCAGGCGT CTCACTTCTT
CTGGGCTCTC TGGGCCCTCA TCCAGAACCA GTACTCCACC ATCGACTTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu13684
Accession Version NM_018208.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1161bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ethanolamine kinase 2 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL592146.8, DB454429.1, BC010082.2 and AA854978.1. On Jul 10, 2014 this sequence version replaced gi:186659524. Summary: The protein encoded by this gene is a member of choline/ethanolamine kinase family which catalyzes the first step of phosphatidylethanolamine (PtdEtn) biosynthesis via the cytidine diphosphate (CDP) ethanolamine pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]. Transcript Variant: This variant (1) encodes isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC010082.2, AB073608.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962343 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGCTGTGC CCCCTTCGGC CCCTCAGCCG CGCGCGTCCT TTCACCTGAG GAGGCACACG 
CCTTGCCCGC AGTGCTCATG GGGCATGGAG GAGAAGGCGG CGGCCAGCGC CAGCTGCCGG
GAGCCGCCGG GCCCCCCGAG GGCCGCCGCC GTCGCGTACT TCGGCATTTC CGTGGACCCG
GACGACATCC TTCCCGGGGC CCTGCGCCTC ATCCAGGAGC TGCGGCCGCA TTGGAAACCC
GAGCAAGTTC GGACCAAGCG CTTCACGGAT GGCATCACCA ACAAGCTGGT GGCCTGCTAT
GTGGAGGAGG ACATGCAGGA CTGCGTGCTG GTCCGGGTGT ATGGGGAGCG GACGGAGCTG
CTGGTGGACC GGGAGAATGA GGTCAGAAAC TTCCAGCTGC TGCGAGCACA CAGCTGTGCC
CCCAAACTCT ACTGCACCTT CCAGAATGGG CTGTGCTATG AGTACATGCA GGGTGTGGCC
CTGGAGCCTG AGCACATCCG TGAGCCCCGG CTTTTCAGGT TAATCGCCTT AGAAATGGCA
AAGATTCATA CTATCCACGC CAACGGCAGC CTGCCCAAGC CCATCCTCTG GCACAAGATG
CACAATTATT TCACGCTTGT GAAGAACGAG ATCAACCCCA GCCTTTCTGC AGATGTCCCT
AAGGTAGAGG TGTTGGAACG GGAGCTGGCC TGGCTGAAGG AGCATCTGTC CCAGCTGGAG
TCCCCTGTGG TGTTTTGTCA CAATGACCTG CTCTGCAAGA ATATCATCTA TGACAGCATC
AAAGGTCACG TGCGGTTCAT TGACTATGAA TATGCTGGCT ACAACTACCA AGCTTTTGAC
ATTGGCAACC ATTTCAATGA GTTTGCAGGC GTGAATGAGG TGGATTACTG CCTGTACCCG
GCGCGGGAGA CCCAGCTGCA GTGGCTGCAC TACTACCTGC AGGCACAAAA GGGGATGGCC
GTGACCCCCA GGGAGGTGCA AAGGCTCTAC GTGCAAGTCA ACAAGTTTGC CCTGGCGTCT
CACTTCTTCT GGGCTCTCTG GGCCCTCATC CAGAACCAGT ACTCCACCAT CGACTTTGAT
TTCCTCAGGT ACGCAGTGAT CCGATTCAAC CAGTACTTCA AGGTGAAGCC TCAAGCGTCA
GCCTTGGAGA TGCCAAAGTG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_060678.2
CDS331..1491
Misc Feature(1)64..66(+)
Misc Feature(2)508..1464(+)
Misc Feature(3)577..1455(+)
Misc Feature(4)595..1206(+)
Misc Feature(5)595..1134(+)
Misc Feature(6)601..1206(+)
Misc Feature(7)982..1455(+)
Exon (1)1..588
Gene:ETNK2
Gene Synonym:
Exon (2)589..848
Gene:ETNK2
Gene Synonym:
Exon (3)849..971
Gene:ETNK2
Gene Synonym:
Exon (4)972..1114
Gene:ETNK2
Gene Synonym:
Exon (5)1115..1198
Gene:ETNK2
Gene Synonym:
Exon (6)1199..1344
Gene:ETNK2
Gene Synonym:
Exon (7)1345..1418
Gene:ETNK2
Gene Synonym:
Exon (8)1419..2614
Gene:ETNK2
Gene Synonym:
Translation
Position Chain Variation Link
74 74 a, g dbSNP:191312273
77 77 c, g dbSNP:553260192
105 105 a, g dbSNP:539709927
154 154 c, t dbSNP:187440692
212 212 a, g dbSNP:550740913
242 242 c, g dbSNP:530942837
249 249 c, t dbSNP:12738682
290 290 a, c dbSNP:568533842
297 297 a, c dbSNP:12738530
313 313 g, t dbSNP:548341006
322 322 a, c dbSNP:776557336
330 330 a, g dbSNP:770609376
343 343 c, g dbSNP:746714627
358 358 a, c dbSNP:772697570
359 359 a, c dbSNP:3737655
361 361 c, t dbSNP:747596168
365 365 c, g dbSNP:755121524
376 376 c, t dbSNP:559449870
412 412 a, g dbSNP:540551989
423 423 -, g dbSNP:751841362
466 466 c, t dbSNP:779395017
468 468 a, g dbSNP:755542077
470 470 g, t dbSNP:749741774
475 475 a, g dbSNP:533238188
480 480 c, t dbSNP:756406936
517 517 a, c dbSNP:564304932
545 545 a, g dbSNP:376162037
590 590 a, g dbSNP:374386002
596 596 c, t dbSNP:760338329
611 611 a, g dbSNP:149143962
618 618 a, g dbSNP:771641428
620 620 c, t dbSNP:747789899
628 628 c, t dbSNP:773906422
629 629 a, g dbSNP:768128714
631 631 a, g dbSNP:370680512
634 634 a, g dbSNP:530687373
654 654 c, t dbSNP:200236107
655 655 a, g dbSNP:746164408
663 663 c, g, t dbSNP:757621574
665 665 a, g dbSNP:751819221
669 669 a, g dbSNP:764309147
680 680 a, g dbSNP:202185285
683 683 c, t dbSNP:528212957
684 684 a, g dbSNP:372992385
689 689 c, t dbSNP:370190989
700 700 c, t dbSNP:761624943
701 701 a, c, g dbSNP:201400955
708 708 g, t dbSNP:761499775
717 717 a, g dbSNP:773965589
724 724 c, t dbSNP:768188076
726 726 c, g dbSNP:762324880
727 727 c, g dbSNP:776081427
733 733 a, c dbSNP:770133377
746 746 a, g dbSNP:745615577
753 753 c, t dbSNP:781729192
760 760 c, t dbSNP:559020342
765 765 c, t dbSNP:370194168
788 788 a, g dbSNP:377403947
792 792 a, g dbSNP:758518203
796 796 a, c, t dbSNP:779042118
801 801 a, g dbSNP:373432247
804 804 c, t dbSNP:200166795
820 820 a, g dbSNP:750335710
825 825 c, t dbSNP:576441238
829 829 c, t dbSNP:141281450
830 830 a, g dbSNP:144504466
837 837 c, t dbSNP:763600102
838 838 c, t dbSNP:762532276
839 839 a, g dbSNP:774930573
853 853 a, g dbSNP:778813214
855 855 c, t dbSNP:768537260
856 856 a, g dbSNP:748976903
868 868 a, g dbSNP:370629139
878 878 a, c dbSNP:755701277
879 879 c, t dbSNP:749884971
886 886 c, t dbSNP:780567647
888 888 c, t dbSNP:139650333
889 889 a, g dbSNP:751988639
894 894 c, g dbSNP:1880534
894 894 c, t dbSNP:764482873
895 895 g, t dbSNP:763265470
927 927 c, g dbSNP:752940406
933 933 c, t dbSNP:765374787
944 944 c, t dbSNP:759671962
945 945 a, g, t dbSNP:758154824
957 957 c, t dbSNP:143999368
958 958 a, c, g dbSNP:768590671
963 963 c, t dbSNP:549075990
980 980 c, t dbSNP:745574189
983 983 a, g dbSNP:373228420
988 988 a, c dbSNP:770497691
994 994 a, g dbSNP:746465912
995 995 c, t dbSNP:777258273
1001 1001 c, t dbSNP:758961107
1002 1002 a, g dbSNP:748630621
1003 1003 c, t dbSNP:779374402
1006 1006 a, g dbSNP:755254782
1009 1009 c, t dbSNP:754108955
1010 1010 a, g, t dbSNP:3737657
1011 1011 a, g dbSNP:138409813
1013 1013 -, a dbSNP:35235705
1014 1014 a, g dbSNP:767695300
1025 1025 c, t dbSNP:763037814
1032 1032 c, g dbSNP:775542456
1038 1038 g, t dbSNP:759163609
1045 1045 a, c dbSNP:200858943
1053 1053 c, g dbSNP:776320731
1056 1056 g, t dbSNP:116285702
1065 1065 c, t dbSNP:770425571
1072 1072 a, g dbSNP:746649734
1078 1078 c, t dbSNP:772756403
1098 1098 c, t dbSNP:771539071
1101 1101 c, t dbSNP:748681339
1119 1119 c, t dbSNP:140913993
1120 1120 a, g dbSNP:374969247
1122 1122 a, g dbSNP:771348515
1123 1123 c, t dbSNP:565275436
1124 1124 a, g, t dbSNP:554543246
1133 1133 a, t dbSNP:749201140
1134 1134 c, t dbSNP:779908230
1146 1146 c, t dbSNP:200739096
1161 1161 a, g dbSNP:750284336
1171 1171 a, g dbSNP:767284095
1172 1172 c, t dbSNP:756911069
1176 1176 a, c dbSNP:751194146
1187 1187 a, g dbSNP:763544137
1200 1200 c, t dbSNP:752325302
1201 1201 a, g dbSNP:533254441
1206 1206 c, t dbSNP:201036824
1209 1209 a, g dbSNP:777234659
1212 1212 a, g dbSNP:375907212
1223 1223 g, t dbSNP:761038426
1225 1225 c, t dbSNP:750538219
1229 1229 a, c, t dbSNP:761926179
1230 1230 a, g, t dbSNP:372782006
1231 1231 c, g dbSNP:745838701
1232 1232 a, c, t dbSNP:570954657
1233 1233 a, g dbSNP:761470297
1234 1234 -, c dbSNP:770142507
1234 1234 c, g, t dbSNP:201980742
1235 1235 a, g dbSNP:200305922
1236 1236 -, cgcgg dbSNP:748320572
1237 1237 a, g dbSNP:778594983
1239 1239 c, g dbSNP:140658518
1241 1241 c, g dbSNP:754406318
1242 1242 c, t dbSNP:766839183
1243 1243 c, t dbSNP:761081739
1244 1244 a, c dbSNP:750856269
1250 1250 a, c dbSNP:767749304
1258 1258 c, g dbSNP:762064107
1259 1259 a, g dbSNP:764023394
1260 1260 c, g dbSNP:61825753
1262 1262 a, g dbSNP:542121001
1272 1272 a, g dbSNP:776441116
1281 1281 a, g dbSNP:770971979
1282 1282 a, g dbSNP:746909963
1284 1284 a, c, g dbSNP:775624367
1285 1285 a, t dbSNP:144439568
1287 1287 a, g dbSNP:747823343
1299 1299 c, t dbSNP:778646158
1308 1308 a, g dbSNP:754536920
1311 1311 a, g dbSNP:149960550
1317 1317 c, t dbSNP:753465248
1320 1320 c, t dbSNP:146679279
1321 1321 a, g dbSNP:756691101
1323 1323 a, g dbSNP:750862892
1330 1330 a, g dbSNP:767957800
1336 1336 g, t dbSNP:762117921
1346 1346 c, t dbSNP:769362056
1347 1347 a, g, t dbSNP:780677236
1355 1355 a, t dbSNP:370655182
1387 1387 c, g dbSNP:747496173
1396 1396 a, c dbSNP:778153843
1401 1401 a, c, t dbSNP:752974385
1402 1402 a, g dbSNP:148772808
1415 1415 a, t dbSNP:755219297
1422 1422 c, t dbSNP:76205451
1424 1424 c, t dbSNP:780309918
1432 1432 c, t dbSNP:756213388
1437 1437 c, g dbSNP:746568489
1461 1461 c, t dbSNP:751557066
1467 1467 a, g dbSNP:764058133
1472 1472 c, t dbSNP:762943597
1479 1479 a, g dbSNP:752493579
1490 1490 a, g dbSNP:371581071
1505 1505 c, t dbSNP:759156287
1511 1511 c, g, t dbSNP:574964789
1512 1512 -, c dbSNP:780402256
1515 1515 c, t dbSNP:368115670
1519 1519 c, t dbSNP:539891992
1525 1525 g, t dbSNP:772509364
1528 1528 a, c dbSNP:748662740
1533 1533 c, g dbSNP:779185057
1559 1559 c, t dbSNP:535590418
1634 1634 a, g dbSNP:775177450
1640 1640 c, g dbSNP:566547978
1648 1648 a, g dbSNP:139047077
1665 1665 c, t dbSNP:539339572
1679 1679 a, g dbSNP:777186551
1689 1689 c, t dbSNP:151131812
1702 1702 c, t dbSNP:763143837
1763 1763 c, t dbSNP:41313900
1767 1767 c, t dbSNP:139891629
1783 1783 a, t dbSNP:759820778
1785 1785 c, t dbSNP:117726554
1831 1831 a, g dbSNP:771663723
1853 1853 a, g dbSNP:77936713
1856 1856 a, c dbSNP:75876713
1857 1857 a, t dbSNP:79403699
1887 1887 a, g dbSNP:549500658
1907 1907 c, g dbSNP:746575421
1968 1968 c, g dbSNP:777135117
1999 1999 g, t dbSNP:529432849
2004 2004 a, g dbSNP:777254623
2011 2011 c, g dbSNP:181336042
2020 2020 a, g dbSNP:771583361
2030 2030 c, g dbSNP:746474734
2054 2054 c, t dbSNP:772816567
2095 2095 c, t dbSNP:76718240
2096 2096 a, g dbSNP:533436486
2115 2115 c, t dbSNP:564460294
2141 2141 c, t dbSNP:543769555
2150 2150 c, g dbSNP:574897142
2202 2202 a, g dbSNP:554992422
2220 2220 g, t dbSNP:541550800
2223 2223 a, g dbSNP:145968121
2237 2237 c, t dbSNP:778495752
2321 2321 c, t dbSNP:754746019
2354 2354 c, t dbSNP:114187399
2412 2412 -, c dbSNP:375255895
2474 2474 c, g dbSNP:539644532
2483 2483 c, g, t dbSNP:557304214
2530 2530 a, g dbSNP:116590745
2549 2549 c, t dbSNP:569270610
2604 2604 c, t dbSNP:780002989

Target ORF information:

RefSeq Version NM_018208
Organism Homo sapiens (human)
Definition Homo sapiens ethanolamine kinase 2 (ETNK2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_018208

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGCTGTGC CCCCTTCGGC CCCTCAGCCG CGCGCGTCCT TTCACCTGAG GAGGCACACG 
CCTTGCCCGC AGTGCTCATG GGGCATGGAG GAGAAGGCGG CGGCCAGCGC CAGCTGCCGG
GAGCCGCCGG GCCCCCCGAG GGCCGCCGCC GTCGCGTACT TCGGCATTTC CGTGGACCCG
GACGACATCC TTCCCGGGGC CCTGCGCCTC ATCCAGGAGC TGCGGCCGCA TTGGAAACCC
GAGCAAGTTC GGACCAAGCG CTTCACGGAT GGCATCACCA ACAAGCTGGT GGCCTGCTAT
GTGGAGGAGG ACATGCAGGA CTGCGTGCTG GTCCGGGTGT ATGGGGAGCG GACGGAGCTG
CTGGTGGACC GGGAGAATGA GGTCAGAAAC TTCCAGCTGC TGCGAGCACA CAGCTGTGCC
CCCAAACTCT ACTGCACCTT CCAGAATGGG CTGTGCTATG AGTACATGCA GGGTGTGGCC
CTGGAGCCTG AGCACATCCG TGAGCCCCGG CTTTTCAGGT TAATCGCCTT AGAAATGGCA
AAGATTCATA CTATCCACGC CAACGGCAGC CTGCCCAAGC CCATCCTCTG GCACAAGATG
CACAATTATT TCACGCTTGT GAAGAACGAG ATCAACCCCA GCCTTTCTGC AGATGTCCCT
AAGGTAGAGG TGTTGGAACG GGAGCTGGCC TGGCTGAAGG AGCATCTGTC CCAGCTGGAG
TCCCCTGTGG TGTTTTGTCA CAATGACCTG CTCTGCAAGA ATATCATCTA TGACAGCATC
AAAGGTCACG TGCGGTTCAT TGACTATGAA TATGCTGGCT ACAACTACCA AGCTTTTGAC
ATTGGCAACC ATTTCAATGA GTTTGCAGGC GTGAATGAGG TGGATTACTG CCTGTACCCG
GCGCGGGAGA CCCAGCTGCA GTGGCTGCAC TACTACCTGC AGGCACAAAA GGGGATGGCC
GTGACCCCCA GGGAGGTGCA AAGGCTCTAC GTGCAAGTCA ACAAGTTTGC CCTGGCGTCT
CACTTCTTCT GGGCTCTCTG GGCCCTCATC CAGAACCAGT ACTCCACCAT CGACTTTGAT
TTCCTCAGGT ACGCAGTGAT CCGATTCAAC CAGTACTTCA AGGTGAAGCC TCAAGCGTCA
GCCTTGGAGA TGCCAAAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu34522
Accession Version NM_001297760.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1185bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ethanolamine kinase 2 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL592146.8, DB454429.1, AK001623.1 and AA854978.1. On Jul 10, 2014 this sequence version replaced gi:530365001. Summary: The protein encoded by this gene is a member of choline/ethanolamine kinase family which catalyzes the first step of phosphatidylethanolamine (PtdEtn) biosynthesis via the cytidine diphosphate (CDP) ethanolamine pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is longer than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK001623.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962343 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGCTGTGC CCCCTTCGGC CCCTCAGCCG CGCGCGTCCT TTCACCTGAG GAGGCACACG 
CCTTGCCCGC AGTGCTCATG GGGCATGGAG GAGAAGGCGG CGGCCAGCGC CAGCTGCCGG
GAGCCGCCGG GCCCCCCGAG GGCCGCCGCC GTCGCGTACT TCGGCATTTC CGTGGACCCG
GACGACATCC TTCCCGGGGC CCTGCGCCTC ATCCAGGAGC TGCGGCCGCA TTGGAAACCC
GAGCAAGTTC GGACCAAGCG CTTCACGGAT GGCATCACCA ACAAGCTGGT GGCCTGCTAT
GTGGAGGAGG ACATGCAGGA CTGCGTGCTG GTCCGGGTGT ATGGGGAGCG GACGGAGCTG
CTGGTGGACC GGGAGAATGA GGTCAGAAAC TTCCAGCTGC TGCGAGCACA CAGCTGTGCC
CCCAAACTCT ACTGCACCTT CCAGAATGGG CTGTGCTATG AGTACATGCA GGGTGTGGCC
CTGGAGCCTG AGCACATCCG TGAGCCCCGG CTTTTCAGGT TAATCGCCTT AGAAATGGCA
AAGATTCATA CTATCCACGC CAACGGCAGC CTGCCCAAGC CCATCCTCTG GCACAAGATG
CACAATTATT TCACGCTTGT GAAGAACGAG ATCAACCCCA GCCTTTCTGC AGATGTCCCT
AAGGTAGAGG TGTTGGAACG GGAGCTGGCC TGGCTGAAGG AGCATCTGTC CCAGCTGGAG
TCCCCTGTGG TGTTTTGTCA CAATGACCTG CTCTGCAAGA ATATCATCTA TGACAGCATC
AAAGGTCACG TGCGGTTCAT TGACTATGAA TATGCTGGCT ACAACTACCA AGCTTTTGAC
ATTGGCAACC ATTTCAATGA GTTTGCAGGC GTGAATGAGG TGGATTACTG CCTGTACCCG
GCGCGGGAGA CCCAGCTGCA GTGGCTGCAC TACTACCTGC AGGCACAAAA GGGGATGGCC
GTGACCCCCA GGGAGGTGCA AAGGCTCTAC GTGCAAGTCA ACAAGTTTGC CCTGGGTCCT
AGCTGTGTGT CTTCCACAAT GACTGCATCC CTCCAGTGCT GTAGAGTCGG AAACAGGCAT
GGGGAGATTG CCAGGCTGAC CCTCTCTGGT CTGTTTCCAG GCGTCTCACT TCTTCTGGGC
TCTCTGGGCC CTCATCCAGA ACCAGTACTC CACCATCGAC TTTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001284689.1
CDS331..1515
Misc Feature(1)64..66(+)
Misc Feature(2)577..1344(+)
Misc Feature(3)595..1206(+)
Misc Feature(4)595..1134(+)
Misc Feature(5)601..1206(+)
Misc Feature(6)982..1344(+)
Exon (1)1..588
Gene:ETNK2
Gene Synonym:
Exon (2)589..848
Gene:ETNK2
Gene Synonym:
Exon (3)849..971
Gene:ETNK2
Gene Synonym:
Exon (4)972..1114
Gene:ETNK2
Gene Synonym:
Exon (5)1115..1198
Gene:ETNK2
Gene Synonym:
Exon (6)1199..1344
Gene:ETNK2
Gene Synonym:
Exon (7)1345..1524
Gene:ETNK2
Gene Synonym:
Exon (8)1525..2720
Gene:ETNK2
Gene Synonym:
Translation
Position Chain Variation Link
74 74 a, g dbSNP:191312273
77 77 c, g dbSNP:553260192
105 105 a, g dbSNP:539709927
154 154 c, t dbSNP:187440692
212 212 a, g dbSNP:550740913
242 242 c, g dbSNP:530942837
249 249 c, t dbSNP:12738682
290 290 a, c dbSNP:568533842
297 297 a, c dbSNP:12738530
313 313 g, t dbSNP:548341006
322 322 a, c dbSNP:776557336
330 330 a, g dbSNP:770609376
343 343 c, g dbSNP:746714627
358 358 a, c dbSNP:772697570
359 359 a, c dbSNP:3737655
361 361 c, t dbSNP:747596168
365 365 c, g dbSNP:755121524
376 376 c, t dbSNP:559449870
412 412 a, g dbSNP:540551989
423 423 -, g dbSNP:751841362
466 466 c, t dbSNP:779395017
468 468 a, g dbSNP:755542077
470 470 g, t dbSNP:749741774
475 475 a, g dbSNP:533238188
480 480 c, t dbSNP:756406936
517 517 a, c dbSNP:564304932
545 545 a, g dbSNP:376162037
590 590 a, g dbSNP:374386002
596 596 c, t dbSNP:760338329
611 611 a, g dbSNP:149143962
618 618 a, g dbSNP:771641428
620 620 c, t dbSNP:747789899
628 628 c, t dbSNP:773906422
629 629 a, g dbSNP:768128714
631 631 a, g dbSNP:370680512
634 634 a, g dbSNP:530687373
654 654 c, t dbSNP:200236107
655 655 a, g dbSNP:746164408
663 663 c, g, t dbSNP:757621574
665 665 a, g dbSNP:751819221
669 669 a, g dbSNP:764309147
680 680 a, g dbSNP:202185285
683 683 c, t dbSNP:528212957
684 684 a, g dbSNP:372992385
689 689 c, t dbSNP:370190989
700 700 c, t dbSNP:761624943
701 701 a, c, g dbSNP:201400955
708 708 g, t dbSNP:761499775
717 717 a, g dbSNP:773965589
724 724 c, t dbSNP:768188076
726 726 c, g dbSNP:762324880
727 727 c, g dbSNP:776081427
733 733 a, c dbSNP:770133377
746 746 a, g dbSNP:745615577
753 753 c, t dbSNP:781729192
760 760 c, t dbSNP:559020342
765 765 c, t dbSNP:370194168
788 788 a, g dbSNP:377403947
792 792 a, g dbSNP:758518203
796 796 a, c, t dbSNP:779042118
801 801 a, g dbSNP:373432247
804 804 c, t dbSNP:200166795
820 820 a, g dbSNP:750335710
825 825 c, t dbSNP:576441238
829 829 c, t dbSNP:141281450
830 830 a, g dbSNP:144504466
837 837 c, t dbSNP:763600102
838 838 c, t dbSNP:762532276
839 839 a, g dbSNP:774930573
853 853 a, g dbSNP:778813214
855 855 c, t dbSNP:768537260
856 856 a, g dbSNP:748976903
868 868 a, g dbSNP:370629139
878 878 a, c dbSNP:755701277
879 879 c, t dbSNP:749884971
886 886 c, t dbSNP:780567647
888 888 c, t dbSNP:139650333
889 889 a, g dbSNP:751988639
894 894 c, g dbSNP:1880534
894 894 c, t dbSNP:764482873
895 895 g, t dbSNP:763265470
927 927 c, g dbSNP:752940406
933 933 c, t dbSNP:765374787
944 944 c, t dbSNP:759671962
945 945 a, g, t dbSNP:758154824
957 957 c, t dbSNP:143999368
958 958 a, c, g dbSNP:768590671
963 963 c, t dbSNP:549075990
980 980 c, t dbSNP:745574189
983 983 a, g dbSNP:373228420
988 988 a, c dbSNP:770497691
994 994 a, g dbSNP:746465912
995 995 c, t dbSNP:777258273
1001 1001 c, t dbSNP:758961107
1002 1002 a, g dbSNP:748630621
1003 1003 c, t dbSNP:779374402
1006 1006 a, g dbSNP:755254782
1009 1009 c, t dbSNP:754108955
1010 1010 a, g, t dbSNP:3737657
1011 1011 a, g dbSNP:138409813
1013 1013 -, a dbSNP:35235705
1014 1014 a, g dbSNP:767695300
1025 1025 c, t dbSNP:763037814
1032 1032 c, g dbSNP:775542456
1038 1038 g, t dbSNP:759163609
1045 1045 a, c dbSNP:200858943
1053 1053 c, g dbSNP:776320731
1056 1056 g, t dbSNP:116285702
1065 1065 c, t dbSNP:770425571
1072 1072 a, g dbSNP:746649734
1078 1078 c, t dbSNP:772756403
1098 1098 c, t dbSNP:771539071
1101 1101 c, t dbSNP:748681339
1119 1119 c, t dbSNP:140913993
1120 1120 a, g dbSNP:374969247
1122 1122 a, g dbSNP:771348515
1123 1123 c, t dbSNP:565275436
1124 1124 a, g, t dbSNP:554543246
1133 1133 a, t dbSNP:749201140
1134 1134 c, t dbSNP:779908230
1146 1146 c, t dbSNP:200739096
1161 1161 a, g dbSNP:750284336
1171 1171 a, g dbSNP:767284095
1172 1172 c, t dbSNP:756911069
1176 1176 a, c dbSNP:751194146
1187 1187 a, g dbSNP:763544137
1200 1200 c, t dbSNP:752325302
1201 1201 a, g dbSNP:533254441
1206 1206 c, t dbSNP:201036824
1209 1209 a, g dbSNP:777234659
1212 1212 a, g dbSNP:375907212
1223 1223 g, t dbSNP:761038426
1225 1225 c, t dbSNP:750538219
1229 1229 a, c, t dbSNP:761926179
1230 1230 a, g, t dbSNP:372782006
1231 1231 c, g dbSNP:745838701
1232 1232 a, c, t dbSNP:570954657
1233 1233 a, g dbSNP:761470297
1234 1234 -, c dbSNP:770142507
1234 1234 c, g, t dbSNP:201980742
1235 1235 a, g dbSNP:200305922
1236 1236 -, cgcgg dbSNP:748320572
1237 1237 a, g dbSNP:778594983
1239 1239 c, g dbSNP:140658518
1241 1241 c, g dbSNP:754406318
1242 1242 c, t dbSNP:766839183
1243 1243 c, t dbSNP:761081739
1244 1244 a, c dbSNP:750856269
1250 1250 a, c dbSNP:767749304
1258 1258 c, g dbSNP:762064107
1259 1259 a, g dbSNP:764023394
1260 1260 c, g dbSNP:61825753
1262 1262 a, g dbSNP:542121001
1272 1272 a, g dbSNP:776441116
1281 1281 a, g dbSNP:770971979
1282 1282 a, g dbSNP:746909963
1284 1284 a, c, g dbSNP:775624367
1285 1285 a, t dbSNP:144439568
1287 1287 a, g dbSNP:747823343
1299 1299 c, t dbSNP:778646158
1308 1308 a, g dbSNP:754536920
1311 1311 a, g dbSNP:149960550
1317 1317 c, t dbSNP:753465248
1320 1320 c, t dbSNP:146679279
1321 1321 a, g dbSNP:756691101
1323 1323 a, g dbSNP:750862892
1330 1330 a, g dbSNP:767957800
1336 1336 g, t dbSNP:762117921
1348 1348 c, t dbSNP:764433504
1350 1350 c, t dbSNP:758671106
1355 1355 a, g dbSNP:777569920
1359 1359 a, g dbSNP:752834882
1370 1370 c, t dbSNP:765377969
1373 1373 c, t dbSNP:114520188
1385 1385 a, c dbSNP:773202231
1395 1395 a, g dbSNP:767625923
1396 1396 a, g dbSNP:761828449
1398 1398 c, t dbSNP:538887924
1399 1399 a, g dbSNP:374814783
1416 1416 c, g dbSNP:34175169
1429 1429 a, g dbSNP:111991093
1435 1435 a, t dbSNP:774957303
1452 1452 c, t dbSNP:769362056
1453 1453 a, g, t dbSNP:780677236
1461 1461 a, t dbSNP:370655182
1493 1493 c, g dbSNP:747496173
1502 1502 a, c dbSNP:778153843
1507 1507 a, c, t dbSNP:752974385
1508 1508 a, g dbSNP:148772808
1521 1521 a, t dbSNP:755219297
1528 1528 c, t dbSNP:76205451
1530 1530 c, t dbSNP:780309918
1538 1538 c, t dbSNP:756213388
1543 1543 c, g dbSNP:746568489
1567 1567 c, t dbSNP:751557066
1573 1573 a, g dbSNP:764058133
1578 1578 c, t dbSNP:762943597
1585 1585 a, g dbSNP:752493579
1596 1596 a, g dbSNP:371581071
1611 1611 c, t dbSNP:759156287
1617 1617 c, g, t dbSNP:574964789
1618 1618 -, c dbSNP:780402256
1621 1621 c, t dbSNP:368115670
1625 1625 c, t dbSNP:539891992
1631 1631 g, t dbSNP:772509364
1634 1634 a, c dbSNP:748662740
1639 1639 c, g dbSNP:779185057
1665 1665 c, t dbSNP:535590418
1740 1740 a, g dbSNP:775177450
1746 1746 c, g dbSNP:566547978
1754 1754 a, g dbSNP:139047077
1771 1771 c, t dbSNP:539339572
1785 1785 a, g dbSNP:777186551
1795 1795 c, t dbSNP:151131812
1808 1808 c, t dbSNP:763143837
1869 1869 c, t dbSNP:41313900
1873 1873 c, t dbSNP:139891629
1889 1889 a, t dbSNP:759820778
1891 1891 c, t dbSNP:117726554
1937 1937 a, g dbSNP:771663723
1959 1959 a, g dbSNP:77936713
1962 1962 a, c dbSNP:75876713
1963 1963 a, t dbSNP:79403699
1993 1993 a, g dbSNP:549500658
2013 2013 c, g dbSNP:746575421
2074 2074 c, g dbSNP:777135117
2105 2105 g, t dbSNP:529432849
2110 2110 a, g dbSNP:777254623
2117 2117 c, g dbSNP:181336042
2126 2126 a, g dbSNP:771583361
2136 2136 c, g dbSNP:746474734
2160 2160 c, t dbSNP:772816567
2201 2201 c, t dbSNP:76718240
2202 2202 a, g dbSNP:533436486
2221 2221 c, t dbSNP:564460294
2247 2247 c, t dbSNP:543769555
2256 2256 c, g dbSNP:574897142
2308 2308 a, g dbSNP:554992422
2326 2326 g, t dbSNP:541550800
2329 2329 a, g dbSNP:145968121
2343 2343 c, t dbSNP:778495752
2427 2427 c, t dbSNP:754746019
2460 2460 c, t dbSNP:114187399
2518 2518 -, c dbSNP:375255895
2580 2580 c, g dbSNP:539644532
2589 2589 c, g, t dbSNP:557304214
2636 2636 a, g dbSNP:116590745
2655 2655 c, t dbSNP:569270610
2710 2710 c, t dbSNP:780002989

Target ORF information:

RefSeq Version NM_001297760
Organism Homo sapiens (human)
Definition Homo sapiens ethanolamine kinase 2 (ETNK2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001297760

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGCTGTGC CCCCTTCGGC CCCTCAGCCG CGCGCGTCCT TTCACCTGAG GAGGCACACG 
CCTTGCCCGC AGTGCTCATG GGGCATGGAG GAGAAGGCGG CGGCCAGCGC CAGCTGCCGG
GAGCCGCCGG GCCCCCCGAG GGCCGCCGCC GTCGCGTACT TCGGCATTTC CGTGGACCCG
GACGACATCC TTCCCGGGGC CCTGCGCCTC ATCCAGGAGC TGCGGCCGCA TTGGAAACCC
GAGCAAGTTC GGACCAAGCG CTTCACGGAT GGCATCACCA ACAAGCTGGT GGCCTGCTAT
GTGGAGGAGG ACATGCAGGA CTGCGTGCTG GTCCGGGTGT ATGGGGAGCG GACGGAGCTG
CTGGTGGACC GGGAGAATGA GGTCAGAAAC TTCCAGCTGC TGCGAGCACA CAGCTGTGCC
CCCAAACTCT ACTGCACCTT CCAGAATGGG CTGTGCTATG AGTACATGCA GGGTGTGGCC
CTGGAGCCTG AGCACATCCG TGAGCCCCGG CTTTTCAGGT TAATCGCCTT AGAAATGGCA
AAGATTCATA CTATCCACGC CAACGGCAGC CTGCCCAAGC CCATCCTCTG GCACAAGATG
CACAATTATT TCACGCTTGT GAAGAACGAG ATCAACCCCA GCCTTTCTGC AGATGTCCCT
AAGGTAGAGG TGTTGGAACG GGAGCTGGCC TGGCTGAAGG AGCATCTGTC CCAGCTGGAG
TCCCCTGTGG TGTTTTGTCA CAATGACCTG CTCTGCAAGA ATATCATCTA TGACAGCATC
AAAGGTCACG TGCGGTTCAT TGACTATGAA TATGCTGGCT ACAACTACCA AGCTTTTGAC
ATTGGCAACC ATTTCAATGA GTTTGCAGGC GTGAATGAGG TGGATTACTG CCTGTACCCG
GCGCGGGAGA CCCAGCTGCA GTGGCTGCAC TACTACCTGC AGGCACAAAA GGGGATGGCC
GTGACCCCCA GGGAGGTGCA AAGGCTCTAC GTGCAAGTCA ACAAGTTTGC CCTGGGTCCT
AGCTGTGTGT CTTCCACAAT GACTGCATCC CTCCAGTGCT GTAGAGTCGG AAACAGGCAT
GGGGAGATTG CCAGGCTGAC CCTCTCTGGT CTGTTTCCAG GCGTCTCACT TCTTCTGGGC
TCTCTGGGCC CTCATCCAGA ACCAGTACTC CACCATCGAC TTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu69191
Accession Version NM_001297761.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 627bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ethanolamine kinase 2 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL592146.8, DB454429.1, AK293426.1, AK001623.1 and AA854978.1. Summary: The protein encoded by this gene is a member of choline/ethanolamine kinase family which catalyzes the first step of phosphatidylethanolamine (PtdEtn) biosynthesis via the cytidine diphosphate (CDP) ethanolamine pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]. Transcript Variant: This variant (3) lacks an alternate exon in the 5' region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK293426.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGGCAAAGA TTCATACTAT CCACGCCAAC GGCAGCCTGC CCAAGCCCAT CCTCTGGCAC 
AAGATGCACA ATTATTTCAC GCTTGTGAAG AACGAGATCA ACCCCAGCCT TTCTGCAGAT
GTCCCTAAGG TAGAGGTGTT GGAACGGGAG CTGGCCTGGC TGAAGGAGCA TCTGTCCCAG
CTGGAGTCCC CTGTGGTGTT TTGTCACAAT GACCTGCTCT GCAAGAATAT CATCTATGAC
AGCATCAAAG GTCACGTGCG GTTCATTGAC TATGAATATG CTGGCTACAA CTACCAAGCT
TTTGACATTG GCAACCATTT CAATGAGTTT GCAGGCGTGA ATGAGGTGGA TTACTGCCTG
TACCCGGCGC GGGAGACCCA GCTGCAGTGG CTGCACTACT ACCTGCAGGC ACAAAAGGGG
ATGGCCGTGA CCCCCAGGGA GGTGCAAAGG CTCTACGTGC AAGTCAACAA GTTTGCCCTG
GCGTCTCACT TCTTCTGGGC TCTCTGGGCC CTCATCCAGA ACCAGTACTC CACCATCGAC
TTTGATTTCC TCAGGTACGC AGTGATCCGA TTCAACCAGT ACTTCAAGGT GAAGCCTCAA
GCGTCAGCCT TGGAGATGCC AAAGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001284690.1
CDS605..1231
Misc Feature(1)377..379(+)
Misc Feature(2)605..1195(+)
Misc Feature(3)722..1195(+)
Misc Feature(4)815..946(+)
Exon (1)1..588
Gene:ETNK2
Gene Synonym:
Exon (2)589..711
Gene:ETNK2
Gene Synonym:
Exon (3)712..854
Gene:ETNK2
Gene Synonym:
Exon (4)855..938
Gene:ETNK2
Gene Synonym:
Exon (5)939..1084
Gene:ETNK2
Gene Synonym:
Exon (6)1085..1158
Gene:ETNK2
Gene Synonym:
Exon (7)1159..2354
Gene:ETNK2
Gene Synonym:
Translation
Position Chain Variation Link
74 74 a, g dbSNP:191312273
77 77 c, g dbSNP:553260192
105 105 a, g dbSNP:539709927
154 154 c, t dbSNP:187440692
212 212 a, g dbSNP:550740913
242 242 c, g dbSNP:530942837
249 249 c, t dbSNP:12738682
290 290 a, c dbSNP:568533842
297 297 a, c dbSNP:12738530
313 313 g, t dbSNP:548341006
322 322 a, c dbSNP:776557336
330 330 a, g dbSNP:770609376
343 343 c, g dbSNP:746714627
358 358 a, c dbSNP:772697570
359 359 a, c dbSNP:3737655
361 361 c, t dbSNP:747596168
365 365 c, g dbSNP:755121524
376 376 c, t dbSNP:559449870
412 412 a, g dbSNP:540551989
423 423 -, g dbSNP:751841362
466 466 c, t dbSNP:779395017
468 468 a, g dbSNP:755542077
470 470 g, t dbSNP:749741774
475 475 a, g dbSNP:533238188
480 480 c, t dbSNP:756406936
517 517 a, c dbSNP:564304932
545 545 a, g dbSNP:376162037
593 593 a, g dbSNP:778813214
595 595 c, t dbSNP:768537260
596 596 a, g dbSNP:748976903
608 608 a, g dbSNP:370629139
618 618 a, c dbSNP:755701277
619 619 c, t dbSNP:749884971
626 626 c, t dbSNP:780567647
628 628 c, t dbSNP:139650333
629 629 a, g dbSNP:751988639
634 634 c, g dbSNP:1880534
634 634 c, t dbSNP:764482873
635 635 g, t dbSNP:763265470
667 667 c, g dbSNP:752940406
673 673 c, t dbSNP:765374787
684 684 c, t dbSNP:759671962
685 685 a, g, t dbSNP:758154824
697 697 c, t dbSNP:143999368
698 698 a, c, g dbSNP:768590671
703 703 c, t dbSNP:549075990
720 720 c, t dbSNP:745574189
723 723 a, g dbSNP:373228420
728 728 a, c dbSNP:770497691
734 734 a, g dbSNP:746465912
735 735 c, t dbSNP:777258273
741 741 c, t dbSNP:758961107
742 742 a, g dbSNP:748630621
743 743 c, t dbSNP:779374402
746 746 a, g dbSNP:755254782
749 749 c, t dbSNP:754108955
750 750 a, g, t dbSNP:3737657
751 751 a, g dbSNP:138409813
753 753 -, a dbSNP:35235705
754 754 a, g dbSNP:767695300
765 765 c, t dbSNP:763037814
772 772 c, g dbSNP:775542456
778 778 g, t dbSNP:759163609
785 785 a, c dbSNP:200858943
793 793 c, g dbSNP:776320731
796 796 g, t dbSNP:116285702
805 805 c, t dbSNP:770425571
812 812 a, g dbSNP:746649734
818 818 c, t dbSNP:772756403
838 838 c, t dbSNP:771539071
841 841 c, t dbSNP:748681339
859 859 c, t dbSNP:140913993
860 860 a, g dbSNP:374969247
862 862 a, g dbSNP:771348515
863 863 c, t dbSNP:565275436
864 864 a, g, t dbSNP:554543246
873 873 a, t dbSNP:749201140
874 874 c, t dbSNP:779908230
886 886 c, t dbSNP:200739096
901 901 a, g dbSNP:750284336
911 911 a, g dbSNP:767284095
912 912 c, t dbSNP:756911069
916 916 a, c dbSNP:751194146
927 927 a, g dbSNP:763544137
940 940 c, t dbSNP:752325302
941 941 a, g dbSNP:533254441
946 946 c, t dbSNP:201036824
949 949 a, g dbSNP:777234659
952 952 a, g dbSNP:375907212
963 963 g, t dbSNP:761038426
965 965 c, t dbSNP:750538219
969 969 a, c, t dbSNP:761926179
970 970 a, g, t dbSNP:372782006
971 971 c, g dbSNP:745838701
972 972 a, c, t dbSNP:570954657
973 973 a, g dbSNP:761470297
974 974 -, c dbSNP:770142507
974 974 c, g, t dbSNP:201980742
975 975 a, g dbSNP:200305922
976 976 -, cgcgg dbSNP:748320572
977 977 a, g dbSNP:778594983
979 979 c, g dbSNP:140658518
981 981 c, g dbSNP:754406318
982 982 c, t dbSNP:766839183
983 983 c, t dbSNP:761081739
984 984 a, c dbSNP:750856269
990 990 a, c dbSNP:767749304
998 998 c, g dbSNP:762064107
999 999 a, g dbSNP:764023394
1000 1000 c, g dbSNP:61825753
1002 1002 a, g dbSNP:542121001
1012 1012 a, g dbSNP:776441116
1021 1021 a, g dbSNP:770971979
1022 1022 a, g dbSNP:746909963
1024 1024 a, c, g dbSNP:775624367
1025 1025 a, t dbSNP:144439568
1027 1027 a, g dbSNP:747823343
1039 1039 c, t dbSNP:778646158
1048 1048 a, g dbSNP:754536920
1051 1051 a, g dbSNP:149960550
1057 1057 c, t dbSNP:753465248
1060 1060 c, t dbSNP:146679279
1061 1061 a, g dbSNP:756691101
1063 1063 a, g dbSNP:750862892
1070 1070 a, g dbSNP:767957800
1076 1076 g, t dbSNP:762117921
1086 1086 c, t dbSNP:769362056
1087 1087 a, g, t dbSNP:780677236
1095 1095 a, t dbSNP:370655182
1127 1127 c, g dbSNP:747496173
1136 1136 a, c dbSNP:778153843
1141 1141 a, c, t dbSNP:752974385
1142 1142 a, g dbSNP:148772808
1155 1155 a, t dbSNP:755219297
1162 1162 c, t dbSNP:76205451
1164 1164 c, t dbSNP:780309918
1172 1172 c, t dbSNP:756213388
1177 1177 c, g dbSNP:746568489
1201 1201 c, t dbSNP:751557066
1207 1207 a, g dbSNP:764058133
1212 1212 c, t dbSNP:762943597
1219 1219 a, g dbSNP:752493579
1230 1230 a, g dbSNP:371581071
1245 1245 c, t dbSNP:759156287
1251 1251 c, g, t dbSNP:574964789
1252 1252 -, c dbSNP:780402256
1255 1255 c, t dbSNP:368115670
1259 1259 c, t dbSNP:539891992
1265 1265 g, t dbSNP:772509364
1268 1268 a, c dbSNP:748662740
1273 1273 c, g dbSNP:779185057
1299 1299 c, t dbSNP:535590418
1374 1374 a, g dbSNP:775177450
1380 1380 c, g dbSNP:566547978
1388 1388 a, g dbSNP:139047077
1405 1405 c, t dbSNP:539339572
1419 1419 a, g dbSNP:777186551
1429 1429 c, t dbSNP:151131812
1442 1442 c, t dbSNP:763143837
1503 1503 c, t dbSNP:41313900
1507 1507 c, t dbSNP:139891629
1523 1523 a, t dbSNP:759820778
1525 1525 c, t dbSNP:117726554
1571 1571 a, g dbSNP:771663723
1593 1593 a, g dbSNP:77936713
1596 1596 a, c dbSNP:75876713
1597 1597 a, t dbSNP:79403699
1627 1627 a, g dbSNP:549500658
1647 1647 c, g dbSNP:746575421
1708 1708 c, g dbSNP:777135117
1739 1739 g, t dbSNP:529432849
1744 1744 a, g dbSNP:777254623
1751 1751 c, g dbSNP:181336042
1760 1760 a, g dbSNP:771583361
1770 1770 c, g dbSNP:746474734
1794 1794 c, t dbSNP:772816567
1835 1835 c, t dbSNP:76718240
1836 1836 a, g dbSNP:533436486
1855 1855 c, t dbSNP:564460294
1881 1881 c, t dbSNP:543769555
1890 1890 c, g dbSNP:574897142
1942 1942 a, g dbSNP:554992422
1960 1960 g, t dbSNP:541550800
1963 1963 a, g dbSNP:145968121
1977 1977 c, t dbSNP:778495752
2061 2061 c, t dbSNP:754746019
2094 2094 c, t dbSNP:114187399
2152 2152 -, c dbSNP:375255895
2214 2214 c, g dbSNP:539644532
2223 2223 c, g, t dbSNP:557304214
2270 2270 a, g dbSNP:116590745
2289 2289 c, t dbSNP:569270610
2344 2344 c, t dbSNP:780002989

Target ORF information:

RefSeq Version NM_001297761
Organism Homo sapiens (human)
Definition Homo sapiens ethanolamine kinase 2 (ETNK2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001297761

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCAAAGA TTCATACTAT CCACGCCAAC GGCAGCCTGC CCAAGCCCAT CCTCTGGCAC 
AAGATGCACA ATTATTTCAC GCTTGTGAAG AACGAGATCA ACCCCAGCCT TTCTGCAGAT
GTCCCTAAGG TAGAGGTGTT GGAACGGGAG CTGGCCTGGC TGAAGGAGCA TCTGTCCCAG
CTGGAGTCCC CTGTGGTGTT TTGTCACAAT GACCTGCTCT GCAAGAATAT CATCTATGAC
AGCATCAAAG GTCACGTGCG GTTCATTGAC TATGAATATG CTGGCTACAA CTACCAAGCT
TTTGACATTG GCAACCATTT CAATGAGTTT GCAGGCGTGA ATGAGGTGGA TTACTGCCTG
TACCCGGCGC GGGAGACCCA GCTGCAGTGG CTGCACTACT ACCTGCAGGC ACAAAAGGGG
ATGGCCGTGA CCCCCAGGGA GGTGCAAAGG CTCTACGTGC AAGTCAACAA GTTTGCCCTG
GCGTCTCACT TCTTCTGGGC TCTCTGGGCC CTCATCCAGA ACCAGTACTC CACCATCGAC
TTTGATTTCC TCAGGTACGC AGTGATCCGA TTCAACCAGT ACTTCAAGGT GAAGCCTCAA
GCGTCAGCCT TGGAGATGCC AAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu34524
Accession Version NM_001297762.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1038bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ethanolamine kinase 2 isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL592146.8, DB454429.1, AK001623.1, AK302145.1 and AA854978.1. On Jul 10, 2014 this sequence version replaced gi:530365005. Summary: The protein encoded by this gene is a member of choline/ethanolamine kinase family which catalyzes the first step of phosphatidylethanolamine (PtdEtn) biosynthesis via the cytidine diphosphate (CDP) ethanolamine pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]. Transcript Variant: This variant (4) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (4) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK302145.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962343 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTGTGC CCCCTTCGGC CCCTCAGCCG CGCGCGTCCT TTCACCTGAG GAGGCACACG 
CCTTGCCCGC AGTGCTCATG GGGCATGGAG GAGAAGGCGG CGGCCAGCGC CAGCTGCCGG
GAGCCGCCGG GCCCCCCGAG GGCCGCCGCC GTCGCGTACT TCGGCATTTC CGTGGACCCG
GACGACATCC TTCCCGGGGC CCTGCGCCTC ATCCAGGAGC TGCGGCCGCA TTGGAAACCC
GAGCAAGTTC GGACCAAGCG CTTCACGGAT GGCATCACCA ACAAGCTGGT GGCCTGCTAT
GTGGAGGAGG ACATGCAGGA CTGCGTGCTG GTCCGGGTGT ATGGGGAGCG GACGGAGCTG
CTGGTGGACC GGGAGAATGA GGTCAGAAAC TTCCAGCTGC TGCGAGCACA CAGCTGTGCC
CCCAAACTCT ACTGCACCTT CCAGAATGGG CTGTGCTATG AGTACATGCA GGGTGTGGCC
CTGGAGCCTG AGCACATCCG TGAGCCCCGG CTTTTCAGCC TTTCTGCAGA TGTCCCTAAG
GTAGAGGTGT TGGAACGGGA GCTGGCCTGG CTGAAGGAGC ATCTGTCCCA GCTGGAGTCC
CCTGTGGTGT TTTGTCACAA TGACCTGCTC TGCAAGAATA TCATCTATGA CAGCATCAAA
GGTCACGTGC GGTTCATTGA CTATGAATAT GCTGGCTACA ACTACCAAGC TTTTGACATT
GGCAACCATT TCAATGAGTT TGCAGGCGTG AATGAGGTGG ATTACTGCCT GTACCCGGCG
CGGGAGACCC AGCTGCAGTG GCTGCACTAC TACCTGCAGG CACAAAAGGG GATGGCCGTG
ACCCCCAGGG AGGTGCAAAG GCTCTACGTG CAAGTCAACA AGTTTGCCCT GGCGTCTCAC
TTCTTCTGGG CTCTCTGGGC CCTCATCCAG AACCAGTACT CCACCATCGA CTTTGATTTC
CTCAGGTACG CAGTGATCCG ATTCAACCAG TACTTCAAGG TGAAGCCTCA AGCGTCAGCC
TTGGAGATGC CAAAGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001284691.1
CDS331..1368
Misc Feature(1)64..66(+)
Misc Feature(2)508..1341(+)
Misc Feature(3)577..1332(+)
Misc Feature(4)595..1083(+)
Misc Feature(5)595..1011(+)
Misc Feature(6)601..1083(+)
Misc Feature(7)859..1332(+)
Exon (1)1..588
Gene:ETNK2
Gene Synonym:
Exon (2)589..848
Gene:ETNK2
Gene Synonym:
Exon (3)849..991
Gene:ETNK2
Gene Synonym:
Exon (4)992..1075
Gene:ETNK2
Gene Synonym:
Exon (5)1076..1221
Gene:ETNK2
Gene Synonym:
Exon (6)1222..1295
Gene:ETNK2
Gene Synonym:
Exon (7)1296..2491
Gene:ETNK2
Gene Synonym:
Translation
Position Chain Variation Link
74 74 a, g dbSNP:191312273
77 77 c, g dbSNP:553260192
105 105 a, g dbSNP:539709927
154 154 c, t dbSNP:187440692
212 212 a, g dbSNP:550740913
242 242 c, g dbSNP:530942837
249 249 c, t dbSNP:12738682
290 290 a, c dbSNP:568533842
297 297 a, c dbSNP:12738530
313 313 g, t dbSNP:548341006
322 322 a, c dbSNP:776557336
330 330 a, g dbSNP:770609376
343 343 c, g dbSNP:746714627
358 358 a, c dbSNP:772697570
359 359 a, c dbSNP:3737655
361 361 c, t dbSNP:747596168
365 365 c, g dbSNP:755121524
376 376 c, t dbSNP:559449870
412 412 a, g dbSNP:540551989
423 423 -, g dbSNP:751841362
466 466 c, t dbSNP:779395017
468 468 a, g dbSNP:755542077
470 470 g, t dbSNP:749741774
475 475 a, g dbSNP:533238188
480 480 c, t dbSNP:756406936
517 517 a, c dbSNP:564304932
545 545 a, g dbSNP:376162037
590 590 a, g dbSNP:374386002
596 596 c, t dbSNP:760338329
611 611 a, g dbSNP:149143962
618 618 a, g dbSNP:771641428
620 620 c, t dbSNP:747789899
628 628 c, t dbSNP:773906422
629 629 a, g dbSNP:768128714
631 631 a, g dbSNP:370680512
634 634 a, g dbSNP:530687373
654 654 c, t dbSNP:200236107
655 655 a, g dbSNP:746164408
663 663 c, g, t dbSNP:757621574
665 665 a, g dbSNP:751819221
669 669 a, g dbSNP:764309147
680 680 a, g dbSNP:202185285
683 683 c, t dbSNP:528212957
684 684 a, g dbSNP:372992385
689 689 c, t dbSNP:370190989
700 700 c, t dbSNP:761624943
701 701 a, c, g dbSNP:201400955
708 708 g, t dbSNP:761499775
717 717 a, g dbSNP:773965589
724 724 c, t dbSNP:768188076
726 726 c, g dbSNP:762324880
727 727 c, g dbSNP:776081427
733 733 a, c dbSNP:770133377
746 746 a, g dbSNP:745615577
753 753 c, t dbSNP:781729192
760 760 c, t dbSNP:559020342
765 765 c, t dbSNP:370194168
788 788 a, g dbSNP:377403947
792 792 a, g dbSNP:758518203
796 796 a, c, t dbSNP:779042118
801 801 a, g dbSNP:373432247
804 804 c, t dbSNP:200166795
820 820 a, g dbSNP:750335710
825 825 c, t dbSNP:576441238
829 829 c, t dbSNP:141281450
830 830 a, g dbSNP:144504466
837 837 c, t dbSNP:763600102
838 838 c, t dbSNP:762532276
839 839 a, g dbSNP:774930573
857 857 c, t dbSNP:745574189
860 860 a, g dbSNP:373228420
865 865 a, c dbSNP:770497691
871 871 a, g dbSNP:746465912
872 872 c, t dbSNP:777258273
878 878 c, t dbSNP:758961107
879 879 a, g dbSNP:748630621
880 880 c, t dbSNP:779374402
883 883 a, g dbSNP:755254782
886 886 c, t dbSNP:754108955
887 887 a, g, t dbSNP:3737657
888 888 a, g dbSNP:138409813
890 890 -, a dbSNP:35235705
891 891 a, g dbSNP:767695300
902 902 c, t dbSNP:763037814
909 909 c, g dbSNP:775542456
915 915 g, t dbSNP:759163609
922 922 a, c dbSNP:200858943
930 930 c, g dbSNP:776320731
933 933 g, t dbSNP:116285702
942 942 c, t dbSNP:770425571
949 949 a, g dbSNP:746649734
955 955 c, t dbSNP:772756403
975 975 c, t dbSNP:771539071
978 978 c, t dbSNP:748681339
996 996 c, t dbSNP:140913993
997 997 a, g dbSNP:374969247
999 999 a, g dbSNP:771348515
1000 1000 c, t dbSNP:565275436
1001 1001 a, g, t dbSNP:554543246
1010 1010 a, t dbSNP:749201140
1011 1011 c, t dbSNP:779908230
1023 1023 c, t dbSNP:200739096
1038 1038 a, g dbSNP:750284336
1048 1048 a, g dbSNP:767284095
1049 1049 c, t dbSNP:756911069
1053 1053 a, c dbSNP:751194146
1064 1064 a, g dbSNP:763544137
1077 1077 c, t dbSNP:752325302
1078 1078 a, g dbSNP:533254441
1083 1083 c, t dbSNP:201036824
1086 1086 a, g dbSNP:777234659
1089 1089 a, g dbSNP:375907212
1100 1100 g, t dbSNP:761038426
1102 1102 c, t dbSNP:750538219
1106 1106 a, c, t dbSNP:761926179
1107 1107 a, g, t dbSNP:372782006
1108 1108 c, g dbSNP:745838701
1109 1109 a, c, t dbSNP:570954657
1110 1110 a, g dbSNP:761470297
1111 1111 -, c dbSNP:770142507
1111 1111 c, g, t dbSNP:201980742
1112 1112 a, g dbSNP:200305922
1113 1113 -, cgcgg dbSNP:748320572
1114 1114 a, g dbSNP:778594983
1116 1116 c, g dbSNP:140658518
1118 1118 c, g dbSNP:754406318
1119 1119 c, t dbSNP:766839183
1120 1120 c, t dbSNP:761081739
1121 1121 a, c dbSNP:750856269
1127 1127 a, c dbSNP:767749304
1135 1135 c, g dbSNP:762064107
1136 1136 a, g dbSNP:764023394
1137 1137 c, g dbSNP:61825753
1139 1139 a, g dbSNP:542121001
1149 1149 a, g dbSNP:776441116
1158 1158 a, g dbSNP:770971979
1159 1159 a, g dbSNP:746909963
1161 1161 a, c, g dbSNP:775624367
1162 1162 a, t dbSNP:144439568
1164 1164 a, g dbSNP:747823343
1176 1176 c, t dbSNP:778646158
1185 1185 a, g dbSNP:754536920
1188 1188 a, g dbSNP:149960550
1194 1194 c, t dbSNP:753465248
1197 1197 c, t dbSNP:146679279
1198 1198 a, g dbSNP:756691101
1200 1200 a, g dbSNP:750862892
1207 1207 a, g dbSNP:767957800
1213 1213 g, t dbSNP:762117921
1223 1223 c, t dbSNP:769362056
1224 1224 a, g, t dbSNP:780677236
1232 1232 a, t dbSNP:370655182
1264 1264 c, g dbSNP:747496173
1273 1273 a, c dbSNP:778153843
1278 1278 a, c, t dbSNP:752974385
1279 1279 a, g dbSNP:148772808
1292 1292 a, t dbSNP:755219297
1299 1299 c, t dbSNP:76205451
1301 1301 c, t dbSNP:780309918
1309 1309 c, t dbSNP:756213388
1314 1314 c, g dbSNP:746568489
1338 1338 c, t dbSNP:751557066
1344 1344 a, g dbSNP:764058133
1349 1349 c, t dbSNP:762943597
1356 1356 a, g dbSNP:752493579
1367 1367 a, g dbSNP:371581071
1382 1382 c, t dbSNP:759156287
1388 1388 c, g, t dbSNP:574964789
1389 1389 -, c dbSNP:780402256
1392 1392 c, t dbSNP:368115670
1396 1396 c, t dbSNP:539891992
1402 1402 g, t dbSNP:772509364
1405 1405 a, c dbSNP:748662740
1410 1410 c, g dbSNP:779185057
1436 1436 c, t dbSNP:535590418
1511 1511 a, g dbSNP:775177450
1517 1517 c, g dbSNP:566547978
1525 1525 a, g dbSNP:139047077
1542 1542 c, t dbSNP:539339572
1556 1556 a, g dbSNP:777186551
1566 1566 c, t dbSNP:151131812
1579 1579 c, t dbSNP:763143837
1640 1640 c, t dbSNP:41313900
1644 1644 c, t dbSNP:139891629
1660 1660 a, t dbSNP:759820778
1662 1662 c, t dbSNP:117726554
1708 1708 a, g dbSNP:771663723
1730 1730 a, g dbSNP:77936713
1733 1733 a, c dbSNP:75876713
1734 1734 a, t dbSNP:79403699
1764 1764 a, g dbSNP:549500658
1784 1784 c, g dbSNP:746575421
1845 1845 c, g dbSNP:777135117
1876 1876 g, t dbSNP:529432849
1881 1881 a, g dbSNP:777254623
1888 1888 c, g dbSNP:181336042
1897 1897 a, g dbSNP:771583361
1907 1907 c, g dbSNP:746474734
1931 1931 c, t dbSNP:772816567
1972 1972 c, t dbSNP:76718240
1973 1973 a, g dbSNP:533436486
1992 1992 c, t dbSNP:564460294
2018 2018 c, t dbSNP:543769555
2027 2027 c, g dbSNP:574897142
2079 2079 a, g dbSNP:554992422
2097 2097 g, t dbSNP:541550800
2100 2100 a, g dbSNP:145968121
2114 2114 c, t dbSNP:778495752
2198 2198 c, t dbSNP:754746019
2231 2231 c, t dbSNP:114187399
2289 2289 -, c dbSNP:375255895
2351 2351 c, g dbSNP:539644532
2360 2360 c, g, t dbSNP:557304214
2407 2407 a, g dbSNP:116590745
2426 2426 c, t dbSNP:569270610
2481 2481 c, t dbSNP:780002989

Target ORF information:

RefSeq Version NM_001297762
Organism Homo sapiens (human)
Definition Homo sapiens ethanolamine kinase 2 (ETNK2), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001297762

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTGTGC CCCCTTCGGC CCCTCAGCCG CGCGCGTCCT TTCACCTGAG GAGGCACACG 
CCTTGCCCGC AGTGCTCATG GGGCATGGAG GAGAAGGCGG CGGCCAGCGC CAGCTGCCGG
GAGCCGCCGG GCCCCCCGAG GGCCGCCGCC GTCGCGTACT TCGGCATTTC CGTGGACCCG
GACGACATCC TTCCCGGGGC CCTGCGCCTC ATCCAGGAGC TGCGGCCGCA TTGGAAACCC
GAGCAAGTTC GGACCAAGCG CTTCACGGAT GGCATCACCA ACAAGCTGGT GGCCTGCTAT
GTGGAGGAGG ACATGCAGGA CTGCGTGCTG GTCCGGGTGT ATGGGGAGCG GACGGAGCTG
CTGGTGGACC GGGAGAATGA GGTCAGAAAC TTCCAGCTGC TGCGAGCACA CAGCTGTGCC
CCCAAACTCT ACTGCACCTT CCAGAATGGG CTGTGCTATG AGTACATGCA GGGTGTGGCC
CTGGAGCCTG AGCACATCCG TGAGCCCCGG CTTTTCAGCC TTTCTGCAGA TGTCCCTAAG
GTAGAGGTGT TGGAACGGGA GCTGGCCTGG CTGAAGGAGC ATCTGTCCCA GCTGGAGTCC
CCTGTGGTGT TTTGTCACAA TGACCTGCTC TGCAAGAATA TCATCTATGA CAGCATCAAA
GGTCACGTGC GGTTCATTGA CTATGAATAT GCTGGCTACA ACTACCAAGC TTTTGACATT
GGCAACCATT TCAATGAGTT TGCAGGCGTG AATGAGGTGG ATTACTGCCT GTACCCGGCG
CGGGAGACCC AGCTGCAGTG GCTGCACTAC TACCTGCAGG CACAAAAGGG GATGGCCGTG
ACCCCCAGGG AGGTGCAAAG GCTCTACGTG CAAGTCAACA AGTTTGCCCT GGCGTCTCAC
TTCTTCTGGG CTCTCTGGGC CCTCATCCAG AACCAGTACT CCACCATCGA CTTTGATTTC
CTCAGGTACG CAGTGATCCG ATTCAACCAG TACTTCAAGG TGAAGCCTCA AGCGTCAGCC
TTGGAGATGC CAAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.