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ACER3 alkaline ceramidase 3 [Homo sapiens (human)]

Gene Symbol ACER3
Entrez Gene ID 55331
Full Name alkaline ceramidase 3
Synonyms APHC, PHCA
General protein information
Preferred Names
alkaline ceramidase 3
Names
alkaline ceramidase 3
alkCDase 3
alkaline CDase 3
alkaline phytoceramidase
phytoceramidase, alkaline
alkaline dihydroceramidase SB89
NP_060837.3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11q13.5

Summary lac of sum
Disorder MIM:

Disorder Html:





The following ACER3 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACER3 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu69360 XM_011545151 PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu45421 XM_005274087 PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu45421 XM_011545152 PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu45423 XM_005274092 PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99
OHu45423 XM_011545153 PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99
OHu69361 XM_011545154 PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99
OHu02784 NM_018367 Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu45422 NM_001300954 Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99
OHu45422 NM_001300955 Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99
OHu69362 NM_001300953 Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu69360
Accession Version XM_011545151.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 702bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alkaline ceramidase 3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167190.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTCCGG CCGCGGACCG AGAGGGCTAC TGGGGCCCCA CGACCTCCAC GCTGGACTGG 
TGCGAGGAGA ACTACTCCGT GACCTGGTAC ATCGCCGAGT TCTGGAATAC AGTGAGTAAC
CTGATCATGA TTATACCTCC AATGTTCGGT GCAGTTCAGA GTGTTAGAGA CGGTCTGGAA
AAGCGGTACA TTGCTTCTTA TTTAGCACTC ACAGTGGTAG GAATGGGATC CTGGTGCTTC
CACATGACTC TGAAATATGA AATGCAGCTA TTGGATGAAC TCCCAATGAT ATACAGCTGT
TGCATATTTG TGTACTGCAT GTTTGAATGT TTCAAGATCA AGAACTCAGT AAACTACCAT
CTGCTTTTTA CCTTAGTTCT ATTCAGTTTA ATAGTAACCA CAGTTTACCT TAAGGTAAAA
GAGCCGATAT TCCATCAGGT CATGTATGGA ATGTTGGTCT TTACATTAGT ACTTCGATCT
ATTTATATTG TTACATGGAA CTTTCGAAAG AAGGTACCAC CTATCATAGG TATTACCACA
CAATTTCATG CATGGTGGCA TATTTTAACT GGCCTTGGTT CCTATCTTCA CATCCTTTTC
AGTTTGTATA CAAGAACACT TTACCTGAGA TATAGGCCAA AAGTGAAGTT TCTCTTTGGA
ATCTGGCCAG TGATCCTGTT TGAGCCTCTC AGGAAGCATT GA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011543453.1
CDS71..772
Misc Feature(1)92..745(+)
Translation
Position Chain Variation Link
21 21 c, g dbSNP:754875567
26 26 c, t dbSNP:369558733
30 30 c, t dbSNP:778445091
49 49 -, ggc dbSNP:757425734
50 50 -, ggcggcggc dbSNP:765346972
50 50 -, ggc dbSNP:753848905
56 56 a, g dbSNP:747633542
58 58 c, t dbSNP:763435154
59 59 a, g dbSNP:374042980
68 68 a, g dbSNP:376920235
74 74 c, g dbSNP:777604778
80 80 a, g dbSNP:746227737
88 88 c, t dbSNP:770336801
94 94 c, g dbSNP:776004671
97 97 c, t dbSNP:749764911
105 105 a, g dbSNP:370824515
115 115 c, t dbSNP:138662843
150 150 a, t dbSNP:201190641
152 152 a, g dbSNP:774470717
153 153 a, c dbSNP:149293091
195 195 a, t dbSNP:773286511
206 206 c, t dbSNP:760844035
212 212 a, g dbSNP:754019271
217 217 c, t dbSNP:776464379
218 218 a, g, t dbSNP:372314534
224 224 a, g dbSNP:4379869
233 233 c, g dbSNP:762995174
240 240 a, g dbSNP:763660769
241 241 c, t dbSNP:751139978
242 242 a, g dbSNP:756951583
248 248 -, a dbSNP:781710875
248 248 c, g dbSNP:781035647
252 252 a, g dbSNP:555155221
253 253 a, g dbSNP:4479014
255 255 a, g dbSNP:755357196
260 260 a, g dbSNP:142469431
268 268 c, t dbSNP:748538912
272 272 c, t dbSNP:772596848
277 277 a, g dbSNP:777780701
279 279 c, t dbSNP:113648802
295 295 a, g dbSNP:530818806
313 313 c, t dbSNP:774489309
347 347 a, g dbSNP:372226995
358 358 a, g, t dbSNP:201974871
359 359 a, t dbSNP:776323067
363 363 a, g dbSNP:772891253
373 373 c, t dbSNP:748773593
387 387 c, g dbSNP:759196304
390 390 c, t dbSNP:764372246
397 397 a, c dbSNP:777832951
411 411 a, g dbSNP:746486966
428 428 c, t dbSNP:79316237
442 442 c, t dbSNP:370822453
451 451 a, g dbSNP:759145370
460 460 a, g dbSNP:769455875
463 463 a, g dbSNP:774669854
469 469 a, c dbSNP:17417894
472 472 a, g dbSNP:145982324
496 496 a, c, g dbSNP:3740767
504 504 a, g dbSNP:761198154
546 546 a, g dbSNP:200663793
910 910 a, c dbSNP:12786211
926 926 a, t dbSNP:6592695
1604 1604 c, t dbSNP:767636567
1621 1621 a, g dbSNP:752779765
1709 1709 a, g dbSNP:7925774
1716 1716 g, t dbSNP:754024397
1845 1845 a, g dbSNP:113193324
1931 1931 c, t dbSNP:779767253
2147 2147 c, t dbSNP:591043
2185 2185 c, t dbSNP:1062170
2350 2350 c, t dbSNP:7123496
2425 2425 a, g dbSNP:10793231
2714 2714 a, c dbSNP:17135325
3231 3231 c, t dbSNP:144358416
3248 3248 c, t dbSNP:2826
3272 3272 c, t dbSNP:79522926
3323 3323 a, g dbSNP:200178452
3328 3328 a, c, g dbSNP:202111355
3406 3406 c, t dbSNP:4944134
3533 3533 g, t dbSNP:112256825
3652 3652 a, g dbSNP:7127936
3663 3663 a, g dbSNP:58717726
3730 3730 a, g dbSNP:7128038
3823 3823 a, g dbSNP:73491478

Target ORF information:

RefSeq Version XM_011545151
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011545151

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTCCGG CCGCGGACCG AGAGGGCTAC TGGGGCCCCA CGACCTCCAC GCTGGACTGG 
TGCGAGGAGA ACTACTCCGT GACCTGGTAC ATCGCCGAGT TCTGGAATAC AGTGAGTAAC
CTGATCATGA TTATACCTCC AATGTTCGGT GCAGTTCAGA GTGTTAGAGA CGGTCTGGAA
AAGCGGTACA TTGCTTCTTA TTTAGCACTC ACAGTGGTAG GAATGGGATC CTGGTGCTTC
CACATGACTC TGAAATATGA AATGCAGCTA TTGGATGAAC TCCCAATGAT ATACAGCTGT
TGCATATTTG TGTACTGCAT GTTTGAATGT TTCAAGATCA AGAACTCAGT AAACTACCAT
CTGCTTTTTA CCTTAGTTCT ATTCAGTTTA ATAGTAACCA CAGTTTACCT TAAGGTAAAA
GAGCCGATAT TCCATCAGGT CATGTATGGA ATGTTGGTCT TTACATTAGT ACTTCGATCT
ATTTATATTG TTACATGGAA CTTTCGAAAG AAGGTACCAC CTATCATAGG TATTACCACA
CAATTTCATG CATGGTGGCA TATTTTAACT GGCCTTGGTT CCTATCTTCA CATCCTTTTC
AGTTTGTATA CAAGAACACT TTACCTGAGA TATAGGCCAA AAGTGAAGTT TCTCTTTGGA
ATCTGGCCAG TGATCCTGTT TGAGCCTCTC AGGAAGCATT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu45421
Accession Version XM_005274087.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 678bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alkaline ceramidase 3 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167190.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578821606. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGATTATAC CTCCAATGTT CGGTGCAGTT CAGAGTGTTA GAGACGGTCT GGAAAAGCGG 
TACATTGCTT CTTATTTAGC ACTCACAGTG GTAGGAATGG GATCCTGGTG CTTCCACATG
ACTCTGAAAT ATGAAATGCA GCTATTGGAT GAACTCCCAA TGATATACAG CTGTTGCATA
TTTGTGTACT GCATGTTTGA ATGTTTCAAG ATCAAGAACT CAGTAAACTA CCATCTGCTT
TTTACCTTAG TTCTATTCAG TTTAATAGTA ACCACAGTTT ACCTTAAGGT AAAAGAGCCG
ATATTCCATC AGGTCATGTA TGGAATGTTG GTCTTTACAT TAGTACTTCG ATCTATTTAT
ATTGTTACAT GGGTTTATCC ATGGCTTAGA GGACTGGGTT ATACATCATT GGGTATATTT
TTATTGGGAT TTTTATTTTG GAATATAGAT AACATATTTT GTGAGTCACT GAGGAACTTT
CGAAAGAAGG TACCACCTAT CATAGGTATT ACCACACAAT TTCATGCATG GTGGCATATT
TTAACTGGCC TTGGTTCCTA TCTTCACATC CTTTTCAGTT TGTATACAAG AACACTTTAC
CTGAGATATA GGCCAAAAGT GAAGTTTCTC TTTGGAATCT GGCCAGTGAT CCTGTTTGAG
CCTCTCAGGA AGCATTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005274144.1
CDS315..992
Misc Feature(1)315..965(+)
Translation
Position Chain Variation Link
19 19 -, ggc dbSNP:757425734
20 20 -, ggcggcggc dbSNP:765346972
20 20 -, ggc dbSNP:753848905
26 26 a, g dbSNP:747633542
28 28 c, t dbSNP:763435154
29 29 a, g dbSNP:374042980
38 38 a, g dbSNP:376920235
44 44 c, g dbSNP:777604778
50 50 a, g dbSNP:746227737
58 58 c, t dbSNP:770336801
64 64 c, g dbSNP:776004671
67 67 c, t dbSNP:749764911
75 75 a, g dbSNP:370824515
85 85 c, t dbSNP:138662843
120 120 a, t dbSNP:201190641
122 122 a, g dbSNP:774470717
123 123 a, c dbSNP:149293091
151 151 c, t dbSNP:753661922
167 167 -, a dbSNP:762522595
169 169 -, g dbSNP:780430080
172 172 c, t dbSNP:557299109
187 187 a, g dbSNP:754895200
200 200 a, g dbSNP:573982783
230 230 a, g dbSNP:765136007
251 251 a, t dbSNP:758767426
263 263 a, t dbSNP:527865815
268 268 c, t dbSNP:760008274
274 274 -, t dbSNP:751999596
284 284 a, g dbSNP:771570304
313 313 a, t dbSNP:773286511
324 324 c, t dbSNP:760844035
330 330 a, g dbSNP:754019271
335 335 c, t dbSNP:776464379
336 336 a, g, t dbSNP:372314534
342 342 a, g dbSNP:4379869
351 351 c, g dbSNP:762995174
358 358 a, g dbSNP:763660769
359 359 c, t dbSNP:751139978
360 360 a, g dbSNP:756951583
366 366 -, a dbSNP:781710875
366 366 c, g dbSNP:781035647
370 370 a, g dbSNP:555155221
371 371 a, g dbSNP:4479014
373 373 a, g dbSNP:755357196
378 378 a, g dbSNP:142469431
386 386 c, t dbSNP:748538912
390 390 c, t dbSNP:772596848
395 395 a, g dbSNP:777780701
397 397 c, t dbSNP:113648802
413 413 a, g dbSNP:530818806
431 431 c, t dbSNP:774489309
465 465 a, g dbSNP:372226995
476 476 a, g, t dbSNP:201974871
477 477 a, t dbSNP:776323067
481 481 a, g dbSNP:772891253
491 491 c, t dbSNP:748773593
505 505 c, g dbSNP:759196304
508 508 c, t dbSNP:764372246
515 515 a, c dbSNP:777832951
529 529 a, g dbSNP:746486966
546 546 c, t dbSNP:79316237
560 560 c, t dbSNP:370822453
569 569 a, g dbSNP:759145370
578 578 a, g dbSNP:769455875
581 581 a, g dbSNP:774669854
587 587 a, c dbSNP:17417894
590 590 a, g dbSNP:145982324
614 614 a, c, g dbSNP:3740767
622 622 a, g dbSNP:761198154
664 664 a, g dbSNP:200663793
1130 1130 a, c dbSNP:12786211
1146 1146 a, t dbSNP:6592695
1824 1824 c, t dbSNP:767636567
1841 1841 a, g dbSNP:752779765
1929 1929 a, g dbSNP:7925774
1936 1936 g, t dbSNP:754024397
2065 2065 a, g dbSNP:113193324
2151 2151 c, t dbSNP:779767253
2367 2367 c, t dbSNP:591043
2405 2405 c, t dbSNP:1062170
2570 2570 c, t dbSNP:7123496
2645 2645 a, g dbSNP:10793231
2934 2934 a, c dbSNP:17135325
3451 3451 c, t dbSNP:144358416
3468 3468 c, t dbSNP:2826
3492 3492 c, t dbSNP:79522926
3543 3543 a, g dbSNP:200178452
3548 3548 a, c, g dbSNP:202111355
3626 3626 c, t dbSNP:4944134
3753 3753 g, t dbSNP:112256825
3872 3872 a, g dbSNP:7127936
3883 3883 a, g dbSNP:58717726
3950 3950 a, g dbSNP:7128038
4043 4043 a, g dbSNP:73491478
4466 4466 c, t dbSNP:6592696
4519 4519 a, c dbSNP:143377420
4535 4535 a, g dbSNP:61056520
4578 4578 a, c dbSNP:7950001
4593 4593 g, t dbSNP:141293002
4607 4607 c, g dbSNP:150227830
4669 4669 a, g dbSNP:12224200
4672 4672 a, g dbSNP:12786191
4678 4678 g, t dbSNP:12786192
4699 4699 a, c dbSNP:12788575
4705 4705 a, c dbSNP:12787874
4732 4732 c, t dbSNP:12787895
4739 4739 a, c dbSNP:12788596
4766 4766 -, a dbSNP:56406202
4768 4768 a, g dbSNP:12788609
4821 4821 a, g dbSNP:12363416
4837 4837 -, a dbSNP:57808641
4899 4899 a, g dbSNP:751366368
4935 4935 a, c dbSNP:199742172
4936 4936 a, t dbSNP:200335349
4937 4937 a, c dbSNP:201531878
4954 4954 -, aaaaaaaaaaaaa dbSNP:61767069
5099 5099 a, c dbSNP:76524197
5109 5109 a, g dbSNP:11237059
5145 5145 a, g dbSNP:11237060
5150 5150 a, g dbSNP:754778703
5315 5315 a, g dbSNP:78476665
5363 5363 c, t dbSNP:79463399
5384 5384 a, t dbSNP:10899344
6032 6032 g, t dbSNP:748950171
6077 6077 a, t dbSNP:1989463
6081 6081 a, t dbSNP:12801243
6743 6743 c, t dbSNP:111746566
6866 6866 a, g dbSNP:1945085
6927 6927 c, t dbSNP:199847342
6927 6927 -, t dbSNP:559748271
6928 6928 c, t dbSNP:200517484
7126 7126 a, t dbSNP:118100153

Target ORF information:

RefSeq Version XM_005274087
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005274087

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGATTATAC CTCCAATGTT CGGTGCAGTT CAGAGTGTTA GAGACGGTCT GGAAAAGCGG 
TACATTGCTT CTTATTTAGC ACTCACAGTG GTAGGAATGG GATCCTGGTG CTTCCACATG
ACTCTGAAAT ATGAAATGCA GCTATTGGAT GAACTCCCAA TGATATACAG CTGTTGCATA
TTTGTGTACT GCATGTTTGA ATGTTTCAAG ATCAAGAACT CAGTAAACTA CCATCTGCTT
TTTACCTTAG TTCTATTCAG TTTAATAGTA ACCACAGTTT ACCTTAAGGT AAAAGAGCCG
ATATTCCATC AGGTCATGTA TGGAATGTTG GTCTTTACAT TAGTACTTCG ATCTATTTAT
ATTGTTACAT GGGTTTATCC ATGGCTTAGA GGACTGGGTT ATACATCATT GGGTATATTT
TTATTGGGAT TTTTATTTTG GAATATAGAT AACATATTTT GTGAGTCACT GAGGAACTTT
CGAAAGAAGG TACCACCTAT CATAGGTATT ACCACACAAT TTCATGCATG GTGGCATATT
TTAACTGGCC TTGGTTCCTA TCTTCACATC CTTTTCAGTT TGTATACAAG AACACTTTAC
CTGAGATATA GGCCAAAAGT GAAGTTTCTC TTTGGAATCT GGCCAGTGAT CCTGTTTGAG
CCTCTCAGGA AGCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu45421
Accession Version XM_011545152.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 678bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alkaline ceramidase 3 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167190.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGATTATAC CTCCAATGTT CGGTGCAGTT CAGAGTGTTA GAGACGGTCT GGAAAAGCGG 
TACATTGCTT CTTATTTAGC ACTCACAGTG GTAGGAATGG GATCCTGGTG CTTCCACATG
ACTCTGAAAT ATGAAATGCA GCTATTGGAT GAACTCCCAA TGATATACAG CTGTTGCATA
TTTGTGTACT GCATGTTTGA ATGTTTCAAG ATCAAGAACT CAGTAAACTA CCATCTGCTT
TTTACCTTAG TTCTATTCAG TTTAATAGTA ACCACAGTTT ACCTTAAGGT AAAAGAGCCG
ATATTCCATC AGGTCATGTA TGGAATGTTG GTCTTTACAT TAGTACTTCG ATCTATTTAT
ATTGTTACAT GGGTTTATCC ATGGCTTAGA GGACTGGGTT ATACATCATT GGGTATATTT
TTATTGGGAT TTTTATTTTG GAATATAGAT AACATATTTT GTGAGTCACT GAGGAACTTT
CGAAAGAAGG TACCACCTAT CATAGGTATT ACCACACAAT TTCATGCATG GTGGCATATT
TTAACTGGCC TTGGTTCCTA TCTTCACATC CTTTTCAGTT TGTATACAAG AACACTTTAC
CTGAGATATA GGCCAAAAGT GAAGTTTCTC TTTGGAATCT GGCCAGTGAT CCTGTTTGAG
CCTCTCAGGA AGCATTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011543454.1
CDS125..802
Misc Feature(1)125..775(+)
Translation
Position Chain Variation Link
11 11 c, t dbSNP:527718918
14 14 c, t dbSNP:547888946
82 82 a, g dbSNP:571004012
100 100 a, t dbSNP:10899314
123 123 a, t dbSNP:773286511
134 134 c, t dbSNP:760844035
140 140 a, g dbSNP:754019271
145 145 c, t dbSNP:776464379
146 146 a, g, t dbSNP:372314534
152 152 a, g dbSNP:4379869
161 161 c, g dbSNP:762995174
168 168 a, g dbSNP:763660769
169 169 c, t dbSNP:751139978
170 170 a, g dbSNP:756951583
176 176 -, a dbSNP:781710875
176 176 c, g dbSNP:781035647
180 180 a, g dbSNP:555155221
181 181 a, g dbSNP:4479014
183 183 a, g dbSNP:755357196
188 188 a, g dbSNP:142469431
196 196 c, t dbSNP:748538912
200 200 c, t dbSNP:772596848
205 205 a, g dbSNP:777780701
207 207 c, t dbSNP:113648802
223 223 a, g dbSNP:530818806
241 241 c, t dbSNP:774489309
275 275 a, g dbSNP:372226995
286 286 a, g, t dbSNP:201974871
287 287 a, t dbSNP:776323067
291 291 a, g dbSNP:772891253
301 301 c, t dbSNP:748773593
315 315 c, g dbSNP:759196304
318 318 c, t dbSNP:764372246
325 325 a, c dbSNP:777832951
339 339 a, g dbSNP:746486966
356 356 c, t dbSNP:79316237
370 370 c, t dbSNP:370822453
379 379 a, g dbSNP:759145370
388 388 a, g dbSNP:769455875
391 391 a, g dbSNP:774669854
397 397 a, c dbSNP:17417894
400 400 a, g dbSNP:145982324
424 424 a, c, g dbSNP:3740767
432 432 a, g dbSNP:761198154
474 474 a, g dbSNP:200663793
940 940 a, c dbSNP:12786211
956 956 a, t dbSNP:6592695
1634 1634 c, t dbSNP:767636567
1651 1651 a, g dbSNP:752779765
1739 1739 a, g dbSNP:7925774
1746 1746 g, t dbSNP:754024397
1875 1875 a, g dbSNP:113193324
1961 1961 c, t dbSNP:779767253
2177 2177 c, t dbSNP:591043
2215 2215 c, t dbSNP:1062170
2380 2380 c, t dbSNP:7123496
2455 2455 a, g dbSNP:10793231
2744 2744 a, c dbSNP:17135325
3261 3261 c, t dbSNP:144358416
3278 3278 c, t dbSNP:2826
3302 3302 c, t dbSNP:79522926
3353 3353 a, g dbSNP:200178452
3358 3358 a, c, g dbSNP:202111355
3436 3436 c, t dbSNP:4944134
3563 3563 g, t dbSNP:112256825
3682 3682 a, g dbSNP:7127936
3693 3693 a, g dbSNP:58717726
3760 3760 a, g dbSNP:7128038
3853 3853 a, g dbSNP:73491478
4276 4276 c, t dbSNP:6592696
4329 4329 a, c dbSNP:143377420
4345 4345 a, g dbSNP:61056520
4388 4388 a, c dbSNP:7950001
4403 4403 g, t dbSNP:141293002
4417 4417 c, g dbSNP:150227830
4479 4479 a, g dbSNP:12224200
4482 4482 a, g dbSNP:12786191
4488 4488 g, t dbSNP:12786192
4509 4509 a, c dbSNP:12788575
4515 4515 a, c dbSNP:12787874
4542 4542 c, t dbSNP:12787895
4549 4549 a, c dbSNP:12788596
4576 4576 -, a dbSNP:56406202
4578 4578 a, g dbSNP:12788609
4631 4631 a, g dbSNP:12363416
4647 4647 -, a dbSNP:57808641
4709 4709 a, g dbSNP:751366368
4745 4745 a, c dbSNP:199742172
4746 4746 a, t dbSNP:200335349
4747 4747 a, c dbSNP:201531878
4764 4764 -, aaaaaaaaaaaaa dbSNP:61767069
4909 4909 a, c dbSNP:76524197
4919 4919 a, g dbSNP:11237059
4955 4955 a, g dbSNP:11237060
4960 4960 a, g dbSNP:754778703
5125 5125 a, g dbSNP:78476665
5173 5173 c, t dbSNP:79463399
5194 5194 a, t dbSNP:10899344
5842 5842 g, t dbSNP:748950171
5887 5887 a, t dbSNP:1989463
5891 5891 a, t dbSNP:12801243
6553 6553 c, t dbSNP:111746566
6676 6676 a, g dbSNP:1945085
6737 6737 c, t dbSNP:199847342
6737 6737 -, t dbSNP:559748271
6738 6738 c, t dbSNP:200517484
6936 6936 a, t dbSNP:118100153

Target ORF information:

RefSeq Version XM_011545152
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011545152

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGATTATAC CTCCAATGTT CGGTGCAGTT CAGAGTGTTA GAGACGGTCT GGAAAAGCGG 
TACATTGCTT CTTATTTAGC ACTCACAGTG GTAGGAATGG GATCCTGGTG CTTCCACATG
ACTCTGAAAT ATGAAATGCA GCTATTGGAT GAACTCCCAA TGATATACAG CTGTTGCATA
TTTGTGTACT GCATGTTTGA ATGTTTCAAG ATCAAGAACT CAGTAAACTA CCATCTGCTT
TTTACCTTAG TTCTATTCAG TTTAATAGTA ACCACAGTTT ACCTTAAGGT AAAAGAGCCG
ATATTCCATC AGGTCATGTA TGGAATGTTG GTCTTTACAT TAGTACTTCG ATCTATTTAT
ATTGTTACAT GGGTTTATCC ATGGCTTAGA GGACTGGGTT ATACATCATT GGGTATATTT
TTATTGGGAT TTTTATTTTG GAATATAGAT AACATATTTT GTGAGTCACT GAGGAACTTT
CGAAAGAAGG TACCACCTAT CATAGGTATT ACCACACAAT TTCATGCATG GTGGCATATT
TTAACTGGCC TTGGTTCCTA TCTTCACATC CTTTTCAGTT TGTATACAAG AACACTTTAC
CTGAGATATA GGCCAAAAGT GAAGTTTCTC TTTGGAATCT GGCCAGTGAT CCTGTTTGAG
CCTCTCAGGA AGCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu45423
Accession Version XM_005274092.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 498bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alkaline ceramidase 3 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167190.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530396977. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
ATGGCTCCGG CCGCGGACCG AGAGGGCTAC TGGGGCCCCA CGACCTCCAC GCTGGACTGG 
TGCGAGGAGA ACTACTCCGT GACCTGGTAC ATCGCCGAGT TCTGGAATAC AGTGAGTAAC
CTGATCATGA TTATACCTCC AATGTTCGGT GCAGTTCAGA GTGTTAGAGA CGGTCTGGAA
AAGCGGTACA TTGCTTCTTA TTTAGCACTC ACAGTGGTAG GAATGGGATC CTGGTGCTTC
CACATGACTC TGAAATATGA AATGCAGCTA TTGGATGAAC TCCCAATGAT ATACAGCTGT
TGCATATTTG TGTACTGCAT GTTTGAATGT TTCAAGATCA AGAACTCAGT AAACTACCAT
CTGCTTTTTA CCTTAGTTCT ATTCAGTTTA ATAGTAACCA CAGTTTACCT TAAGGTAAAA
GAGCCGATAT TCCATCAGGT CATGTATGGA ATGTTGGTCT TTACATTAGT ACTTCGATCT
ATTTATATTG TTACATGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005274149.1
CDS71..568
Misc Feature(1)92..>565(+)
Translation
Position Chain Variation Link
21 21 c, g dbSNP:754875567
26 26 c, t dbSNP:369558733
30 30 c, t dbSNP:778445091
49 49 -, ggc dbSNP:757425734
50 50 -, ggcggcggc dbSNP:765346972
50 50 -, ggc dbSNP:753848905
56 56 a, g dbSNP:747633542
58 58 c, t dbSNP:763435154
59 59 a, g dbSNP:374042980
68 68 a, g dbSNP:376920235
74 74 c, g dbSNP:777604778
80 80 a, g dbSNP:746227737
88 88 c, t dbSNP:770336801
94 94 c, g dbSNP:776004671
97 97 c, t dbSNP:749764911
105 105 a, g dbSNP:370824515
115 115 c, t dbSNP:138662843
150 150 a, t dbSNP:201190641
152 152 a, g dbSNP:774470717
153 153 a, c dbSNP:149293091
195 195 a, t dbSNP:773286511
206 206 c, t dbSNP:760844035
212 212 a, g dbSNP:754019271
217 217 c, t dbSNP:776464379
218 218 a, g, t dbSNP:372314534
224 224 a, g dbSNP:4379869
233 233 c, g dbSNP:762995174
240 240 a, g dbSNP:763660769
241 241 c, t dbSNP:751139978
242 242 a, g dbSNP:756951583
248 248 -, a dbSNP:781710875
248 248 c, g dbSNP:781035647
252 252 a, g dbSNP:555155221
253 253 a, g dbSNP:4479014
255 255 a, g dbSNP:755357196
260 260 a, g dbSNP:142469431
268 268 c, t dbSNP:748538912
272 272 c, t dbSNP:772596848
277 277 a, g dbSNP:777780701
279 279 c, t dbSNP:113648802
295 295 a, g dbSNP:530818806
313 313 c, t dbSNP:774489309
347 347 a, g dbSNP:372226995
358 358 a, g, t dbSNP:201974871
359 359 a, t dbSNP:776323067
363 363 a, g dbSNP:772891253
373 373 c, t dbSNP:748773593
387 387 c, g dbSNP:759196304
390 390 c, t dbSNP:764372246
397 397 a, c dbSNP:777832951
411 411 a, g dbSNP:746486966
428 428 c, t dbSNP:79316237
442 442 c, t dbSNP:370822453
451 451 a, g dbSNP:759145370
460 460 a, g dbSNP:769455875
463 463 a, g dbSNP:774669854
469 469 a, c dbSNP:17417894
472 472 a, g dbSNP:145982324
496 496 a, c, g dbSNP:3740767
504 504 a, g dbSNP:761198154
546 546 a, g dbSNP:200663793

Target ORF information:

RefSeq Version XM_005274092
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005274092

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGCTCCGG CCGCGGACCG AGAGGGCTAC TGGGGCCCCA CGACCTCCAC GCTGGACTGG 
TGCGAGGAGA ACTACTCCGT GACCTGGTAC ATCGCCGAGT TCTGGAATAC AGTGAGTAAC
CTGATCATGA TTATACCTCC AATGTTCGGT GCAGTTCAGA GTGTTAGAGA CGGTCTGGAA
AAGCGGTACA TTGCTTCTTA TTTAGCACTC ACAGTGGTAG GAATGGGATC CTGGTGCTTC
CACATGACTC TGAAATATGA AATGCAGCTA TTGGATGAAC TCCCAATGAT ATACAGCTGT
TGCATATTTG TGTACTGCAT GTTTGAATGT TTCAAGATCA AGAACTCAGT AAACTACCAT
CTGCTTTTTA CCTTAGTTCT ATTCAGTTTA ATAGTAACCA CAGTTTACCT TAAGGTAAAA
GAGCCGATAT TCCATCAGGT CATGTATGGA ATGTTGGTCT TTACATTAGT ACTTCGATCT
ATTTATATTG TTACATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu45423
Accession Version XM_011545153.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 498bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alkaline ceramidase 3 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167190.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
ATGGCTCCGG CCGCGGACCG AGAGGGCTAC TGGGGCCCCA CGACCTCCAC GCTGGACTGG 
TGCGAGGAGA ACTACTCCGT GACCTGGTAC ATCGCCGAGT TCTGGAATAC AGTGAGTAAC
CTGATCATGA TTATACCTCC AATGTTCGGT GCAGTTCAGA GTGTTAGAGA CGGTCTGGAA
AAGCGGTACA TTGCTTCTTA TTTAGCACTC ACAGTGGTAG GAATGGGATC CTGGTGCTTC
CACATGACTC TGAAATATGA AATGCAGCTA TTGGATGAAC TCCCAATGAT ATACAGCTGT
TGCATATTTG TGTACTGCAT GTTTGAATGT TTCAAGATCA AGAACTCAGT AAACTACCAT
CTGCTTTTTA CCTTAGTTCT ATTCAGTTTA ATAGTAACCA CAGTTTACCT TAAGGTAAAA
GAGCCGATAT TCCATCAGGT CATGTATGGA ATGTTGGTCT TTACATTAGT ACTTCGATCT
ATTTATATTG TTACATGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011543455.1
CDS71..568
Misc Feature(1)92..>565(+)
Translation
Position Chain Variation Link
21 21 c, g dbSNP:754875567
26 26 c, t dbSNP:369558733
30 30 c, t dbSNP:778445091
49 49 -, ggc dbSNP:757425734
50 50 -, ggcggcggc dbSNP:765346972
50 50 -, ggc dbSNP:753848905
56 56 a, g dbSNP:747633542
58 58 c, t dbSNP:763435154
59 59 a, g dbSNP:374042980
68 68 a, g dbSNP:376920235
74 74 c, g dbSNP:777604778
80 80 a, g dbSNP:746227737
88 88 c, t dbSNP:770336801
94 94 c, g dbSNP:776004671
97 97 c, t dbSNP:749764911
105 105 a, g dbSNP:370824515
115 115 c, t dbSNP:138662843
150 150 a, t dbSNP:201190641
152 152 a, g dbSNP:774470717
153 153 a, c dbSNP:149293091
195 195 a, t dbSNP:773286511
206 206 c, t dbSNP:760844035
212 212 a, g dbSNP:754019271
217 217 c, t dbSNP:776464379
218 218 a, g, t dbSNP:372314534
224 224 a, g dbSNP:4379869
233 233 c, g dbSNP:762995174
240 240 a, g dbSNP:763660769
241 241 c, t dbSNP:751139978
242 242 a, g dbSNP:756951583
248 248 -, a dbSNP:781710875
248 248 c, g dbSNP:781035647
252 252 a, g dbSNP:555155221
253 253 a, g dbSNP:4479014
255 255 a, g dbSNP:755357196
260 260 a, g dbSNP:142469431
268 268 c, t dbSNP:748538912
272 272 c, t dbSNP:772596848
277 277 a, g dbSNP:777780701
279 279 c, t dbSNP:113648802
295 295 a, g dbSNP:530818806
313 313 c, t dbSNP:774489309
347 347 a, g dbSNP:372226995
358 358 a, g, t dbSNP:201974871
359 359 a, t dbSNP:776323067
363 363 a, g dbSNP:772891253
373 373 c, t dbSNP:748773593
387 387 c, g dbSNP:759196304
390 390 c, t dbSNP:764372246
397 397 a, c dbSNP:777832951
411 411 a, g dbSNP:746486966
428 428 c, t dbSNP:79316237
442 442 c, t dbSNP:370822453
451 451 a, g dbSNP:759145370
460 460 a, g dbSNP:769455875
463 463 a, g dbSNP:774669854
469 469 a, c dbSNP:17417894
472 472 a, g dbSNP:145982324
496 496 a, c, g dbSNP:3740767
504 504 a, g dbSNP:761198154
546 546 a, g dbSNP:200663793

Target ORF information:

RefSeq Version XM_011545153
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011545153

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGCTCCGG CCGCGGACCG AGAGGGCTAC TGGGGCCCCA CGACCTCCAC GCTGGACTGG 
TGCGAGGAGA ACTACTCCGT GACCTGGTAC ATCGCCGAGT TCTGGAATAC AGTGAGTAAC
CTGATCATGA TTATACCTCC AATGTTCGGT GCAGTTCAGA GTGTTAGAGA CGGTCTGGAA
AAGCGGTACA TTGCTTCTTA TTTAGCACTC ACAGTGGTAG GAATGGGATC CTGGTGCTTC
CACATGACTC TGAAATATGA AATGCAGCTA TTGGATGAAC TCCCAATGAT ATACAGCTGT
TGCATATTTG TGTACTGCAT GTTTGAATGT TTCAAGATCA AGAACTCAGT AAACTACCAT
CTGCTTTTTA CCTTAGTTCT ATTCAGTTTA ATAGTAACCA CAGTTTACCT TAAGGTAAAA
GAGCCGATAT TCCATCAGGT CATGTATGGA ATGTTGGTCT TTACATTAGT ACTTCGATCT
ATTTATATTG TTACATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu69361
Accession Version XM_011545154.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 438bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alkaline ceramidase 3 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167190.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
ATGAGAGTAC TACAAAAAGG ACGAGTGCGT GGGATACAAG ACAGGAGATC CAATGAAAGA 
GAGAGACATT CATTTTACCC CACAGAAGAG TATTGCTTTG AACTGCCCCT GAGAGCTGAA
GATTGGCCAT GGGTTTATCC ATGGCTTAGA GGACTGGGTT ATACATCATT GGGTATATTT
TTATTGGGAT TTTTATTTTG GAATATAGAT AACATATTTT GTGAGTCACT GAGGAACTTT
CGAAAGAAGG TACCACCTAT CATAGGTATT ACCACACAAT TTCATGCATG GTGGCATATT
TTAACTGGCC TTGGTTCCTA TCTTCACATC CTTTTCAGTT TGTATACAAG AACACTTTAC
CTGAGATATA GGCCAAAAGT GAAGTTTCTC TTTGGAATCT GGCCAGTGAT CCTGTTTGAG
CCTCTCAGGA AGCATTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011543456.1
CDS653..1090
Misc Feature(1)779..1063(+)
Translation
Position Chain Variation Link
27 27 a, g dbSNP:113151166
105 105 -, a dbSNP:34446950
404 404 c, g dbSNP:763651407
581 581 c, g dbSNP:4016126
600 600 g, t dbSNP:3851191
666 666 a, g dbSNP:746321183
689 689 c, t dbSNP:758575480
1228 1228 a, c dbSNP:12786211
1244 1244 a, t dbSNP:6592695
1922 1922 c, t dbSNP:767636567
1939 1939 a, g dbSNP:752779765
2027 2027 a, g dbSNP:7925774
2034 2034 g, t dbSNP:754024397
2163 2163 a, g dbSNP:113193324
2249 2249 c, t dbSNP:779767253
2465 2465 c, t dbSNP:591043
2503 2503 c, t dbSNP:1062170
2668 2668 c, t dbSNP:7123496
2743 2743 a, g dbSNP:10793231
3032 3032 a, c dbSNP:17135325
3549 3549 c, t dbSNP:144358416
3566 3566 c, t dbSNP:2826
3590 3590 c, t dbSNP:79522926
3641 3641 a, g dbSNP:200178452
3646 3646 a, c, g dbSNP:202111355
3724 3724 c, t dbSNP:4944134
3851 3851 g, t dbSNP:112256825
3970 3970 a, g dbSNP:7127936
3981 3981 a, g dbSNP:58717726
4048 4048 a, g dbSNP:7128038
4141 4141 a, g dbSNP:73491478

Target ORF information:

RefSeq Version XM_011545154
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011545154

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGAGAGTAC TACAAAAAGG ACGAGTGCGT GGGATACAAG ACAGGAGATC CAATGAAAGA 
GAGAGACATT CATTTTACCC CACAGAAGAG TATTGCTTTG AACTGCCCCT GAGAGCTGAA
GATTGGCCAT GGGTTTATCC ATGGCTTAGA GGACTGGGTT ATACATCATT GGGTATATTT
TTATTGGGAT TTTTATTTTG GAATATAGAT AACATATTTT GTGAGTCACT GAGGAACTTT
CGAAAGAAGG TACCACCTAT CATAGGTATT ACCACACAAT TTCATGCATG GTGGCATATT
TTAACTGGCC TTGGTTCCTA TCTTCACATC CTTTTCAGTT TGTATACAAG AACACTTTAC
CTGAGATATA GGCCAAAAGT GAAGTTTCTC TTTGGAATCT GGCCAGTGAT CCTGTTTGAG
CCTCTCAGGA AGCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02784
Accession Version NM_018367.6 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 804bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alkaline ceramidase 3 isoform a
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG702017.1, AK315000.1, DB461413.1, AP002498.3, AP000752.4 and AI885537.1. On Jul 17, 2014 this sequence version replaced gi:170932466. Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AF327353.1, AF214454.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCTCCGG CCGCGGACCG AGAGGGCTAC TGGGGCCCCA CGACCTCCAC GCTGGACTGG 
TGCGAGGAGA ACTACTCCGT GACCTGGTAC ATCGCCGAGT TCTGGAATAC AGTGAGTAAC
CTGATCATGA TTATACCTCC AATGTTCGGT GCAGTTCAGA GTGTTAGAGA CGGTCTGGAA
AAGCGGTACA TTGCTTCTTA TTTAGCACTC ACAGTGGTAG GAATGGGATC CTGGTGCTTC
CACATGACTC TGAAATATGA AATGCAGCTA TTGGATGAAC TCCCAATGAT ATACAGCTGT
TGCATATTTG TGTACTGCAT GTTTGAATGT TTCAAGATCA AGAACTCAGT AAACTACCAT
CTGCTTTTTA CCTTAGTTCT ATTCAGTTTA ATAGTAACCA CAGTTTACCT TAAGGTAAAA
GAGCCGATAT TCCATCAGGT CATGTATGGA ATGTTGGTCT TTACATTAGT ACTTCGATCT
ATTTATATTG TTACATGGGT TTATCCATGG CTTAGAGGAC TGGGTTATAC ATCATTGGGT
ATATTTTTAT TGGGATTTTT ATTTTGGAAT ATAGATAACA TATTTTGTGA GTCACTGAGG
AACTTTCGAA AGAAGGTACC ACCTATCATA GGTATTACCA CACAATTTCA TGCATGGTGG
CATATTTTAA CTGGCCTTGG TTCCTATCTT CACATCCTTT TCAGTTTGTA TACAAGAACA
CTTTACCTGA GATATAGGCC AAAAGTGAAG TTTCTCTTTG GAATCTGGCC AGTGATCCTG
TTTGAGCCTC TCAGGAAGCA TTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_060837.3
CDS105..908
Misc Feature(1)69..71(+)
Misc Feature(2)126..881(+)
Misc Feature(3)189..257(+)
Misc Feature(4)291..356(+)
Misc Feature(5)384..437(+)
Misc Feature(6)456..515(+)
Misc Feature(7)543..611(+)
Misc Feature(8)624..692(+)
Misc Feature(9)750..818(+)
Exon (1)1..207
Gene:ACER3
Gene Synonym:
Exon (2)208..318
Gene:ACER3
Gene Synonym:
Exon (3)319..371
Gene:ACER3
Gene Synonym:
Exon (4)372..424
Gene:ACER3
Gene Synonym:
Exon (5)425..506
Gene:ACER3
Gene Synonym:
Exon (6)507..542
Gene:ACER3
Gene Synonym:
Exon (7)543..601
Gene:ACER3
Gene Synonym:
Exon (8)602..703
Gene:ACER3
Gene Synonym:
Exon (9)704..808
Gene:ACER3
Gene Synonym:
Exon (10)809..854
Gene:ACER3
Gene Synonym:
Exon (11)855..7378
Gene:ACER3
Gene Synonym:
Translation
Position Chain Variation Link
13 13 a, g dbSNP:528589871
55 55 c, g dbSNP:754875567
60 60 c, t dbSNP:369558733
64 64 c, t dbSNP:778445091
83 83 -, ggc dbSNP:757425734
84 84 -, ggcggcggc dbSNP:765346972
84 84 -, ggc dbSNP:753848905
90 90 a, g dbSNP:747633542
92 92 c, t dbSNP:763435154
93 93 a, g dbSNP:374042980
102 102 a, g dbSNP:376920235
108 108 c, g dbSNP:777604778
114 114 a, g dbSNP:746227737
122 122 c, t dbSNP:770336801
128 128 c, g dbSNP:776004671
131 131 c, t dbSNP:749764911
139 139 a, g dbSNP:370824515
149 149 c, t dbSNP:138662843
184 184 a, t dbSNP:201190641
186 186 a, g dbSNP:774470717
187 187 a, c dbSNP:149293091
229 229 a, t dbSNP:773286511
240 240 c, t dbSNP:760844035
246 246 a, g dbSNP:754019271
251 251 c, t dbSNP:776464379
252 252 a, g, t dbSNP:372314534
258 258 a, g dbSNP:4379869
267 267 c, g dbSNP:762995174
274 274 a, g dbSNP:763660769
275 275 c, t dbSNP:751139978
276 276 a, g dbSNP:756951583
282 282 -, a dbSNP:781710875
282 282 c, g dbSNP:781035647
286 286 a, g dbSNP:555155221
287 287 a, g dbSNP:4479014
289 289 a, g dbSNP:755357196
294 294 a, g dbSNP:142469431
302 302 c, t dbSNP:748538912
306 306 c, t dbSNP:772596848
311 311 a, g dbSNP:777780701
313 313 c, t dbSNP:113648802
329 329 a, g dbSNP:530818806
347 347 c, t dbSNP:774489309
381 381 a, g dbSNP:372226995
392 392 a, g, t dbSNP:201974871
393 393 a, t dbSNP:776323067
397 397 a, g dbSNP:772891253
407 407 c, t dbSNP:748773593
421 421 c, g dbSNP:759196304
424 424 c, t dbSNP:764372246
431 431 a, c dbSNP:777832951
445 445 a, g dbSNP:746486966
462 462 c, t dbSNP:79316237
476 476 c, t dbSNP:370822453
485 485 a, g dbSNP:759145370
494 494 a, g dbSNP:769455875
497 497 a, g dbSNP:774669854
503 503 a, c dbSNP:17417894
506 506 a, g dbSNP:145982324
530 530 a, c, g dbSNP:3740767
538 538 a, g dbSNP:761198154
580 580 a, g dbSNP:200663793
1046 1046 a, c dbSNP:12786211
1062 1062 a, t dbSNP:6592695
1740 1740 c, t dbSNP:767636567
1757 1757 a, g dbSNP:752779765
1845 1845 a, g dbSNP:7925774
1852 1852 g, t dbSNP:754024397
1981 1981 a, g dbSNP:113193324
2067 2067 c, t dbSNP:779767253
2283 2283 c, t dbSNP:591043
2321 2321 c, t dbSNP:1062170
2486 2486 c, t dbSNP:7123496
2561 2561 a, g dbSNP:10793231
2850 2850 a, c dbSNP:17135325
3367 3367 c, t dbSNP:144358416
3384 3384 c, t dbSNP:2826
3408 3408 c, t dbSNP:79522926
3459 3459 a, g dbSNP:200178452
3464 3464 a, c, g dbSNP:202111355
3542 3542 c, t dbSNP:4944134
3669 3669 g, t dbSNP:112256825
3788 3788 a, g dbSNP:7127936
3799 3799 a, g dbSNP:58717726
3866 3866 a, g dbSNP:7128038
3959 3959 a, g dbSNP:73491478
4382 4382 c, t dbSNP:6592696
4435 4435 a, c dbSNP:143377420
4451 4451 a, g dbSNP:61056520
4494 4494 a, c dbSNP:7950001
4509 4509 g, t dbSNP:141293002
4523 4523 c, g dbSNP:150227830
4585 4585 a, g dbSNP:12224200
4588 4588 a, g dbSNP:12786191
4594 4594 g, t dbSNP:12786192
4615 4615 a, c dbSNP:12788575
4621 4621 a, c dbSNP:12787874
4648 4648 c, t dbSNP:12787895
4655 4655 a, c dbSNP:12788596
4682 4682 -, a dbSNP:56406202
4684 4684 a, g dbSNP:12788609
4737 4737 a, g dbSNP:12363416
4753 4753 -, a dbSNP:57808641
4815 4815 a, g dbSNP:751366368
4851 4851 a, c dbSNP:199742172
4852 4852 a, t dbSNP:200335349
4853 4853 a, c dbSNP:201531878
4870 4870 -, aaaaaaaaaaaaa dbSNP:61767069
5015 5015 a, c dbSNP:76524197
5025 5025 a, g dbSNP:11237059
5061 5061 a, g dbSNP:11237060
5066 5066 a, g dbSNP:754778703
5231 5231 a, g dbSNP:78476665
5279 5279 c, t dbSNP:79463399
5300 5300 a, t dbSNP:10899344
5948 5948 g, t dbSNP:748950171
5993 5993 a, t dbSNP:1989463
5997 5997 a, t dbSNP:12801243
6659 6659 c, t dbSNP:111746566
6782 6782 a, g dbSNP:1945085
6843 6843 c, t dbSNP:199847342
6843 6843 -, t dbSNP:559748271
6844 6844 c, t dbSNP:200517484
7042 7042 a, t dbSNP:118100153

Target ORF information:

RefSeq Version NM_018367
Organism Homo sapiens (human)
Definition Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_018367

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCTCCGG CCGCGGACCG AGAGGGCTAC TGGGGCCCCA CGACCTCCAC GCTGGACTGG 
TGCGAGGAGA ACTACTCCGT GACCTGGTAC ATCGCCGAGT TCTGGAATAC AGTGAGTAAC
CTGATCATGA TTATACCTCC AATGTTCGGT GCAGTTCAGA GTGTTAGAGA CGGTCTGGAA
AAGCGGTACA TTGCTTCTTA TTTAGCACTC ACAGTGGTAG GAATGGGATC CTGGTGCTTC
CACATGACTC TGAAATATGA AATGCAGCTA TTGGATGAAC TCCCAATGAT ATACAGCTGT
TGCATATTTG TGTACTGCAT GTTTGAATGT TTCAAGATCA AGAACTCAGT AAACTACCAT
CTGCTTTTTA CCTTAGTTCT ATTCAGTTTA ATAGTAACCA CAGTTTACCT TAAGGTAAAA
GAGCCGATAT TCCATCAGGT CATGTATGGA ATGTTGGTCT TTACATTAGT ACTTCGATCT
ATTTATATTG TTACATGGGT TTATCCATGG CTTAGAGGAC TGGGTTATAC ATCATTGGGT
ATATTTTTAT TGGGATTTTT ATTTTGGAAT ATAGATAACA TATTTTGTGA GTCACTGAGG
AACTTTCGAA AGAAGGTACC ACCTATCATA GGTATTACCA CACAATTTCA TGCATGGTGG
CATATTTTAA CTGGCCTTGG TTCCTATCTT CACATCCTTT TCAGTTTGTA TACAAGAACA
CTTTACCTGA GATATAGGCC AAAAGTGAAG TTTCTCTTTG GAATCTGGCC AGTGATCCTG
TTTGAGCCTC TCAGGAAGCA TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu45422
Accession Version NM_001300954.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alkaline ceramidase 3 isoform c
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG702017.1, BC049837.1, AP000752.4 and AI885537.1. On Jul 17, 2014 this sequence version replaced gi:578821607. Transcript Variant: This variant (3) lacks an internal exon and has another exon in its place compared to variant 1. The resulting isoform (c) is shorter at the N-terminus compared to isoform a. Variants 3 and 4 both encode the same isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC049837.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGATATACA GCTGTTGCAT ATTTGTGTAC TGCATGTTTG AATGTTTCAA GATCAAGAAC 
TCAGTAAACT ACCATCTGCT TTTTACCTTA GTTCTATTCA GTTTAATAGT AACCACAGTT
TACCTTAAGG TAAAAGAGCC GATATTCCAT CAGGTCATGT ATGGAATGTT GGTCTTTACA
TTAGTACTTC GATCTATTTA TATTGTTACA TGGGTTTATC CATGGCTTAG AGGACTGGGT
TATACATCAT TGGGTATATT TTTATTGGGA TTTTTATTTT GGAATATAGA TAACATATTT
TGTGAGTCAC TGAGGAACTT TCGAAAGAAG GTACCACCTA TCATAGGTAT TACCACACAA
TTTCATGCAT GGTGGCATAT TTTAACTGGC CTTGGTTCCT ATCTTCACAT CCTTTTCAGT
TTGTATACAA GAACACTTTA CCTGAGATAT AGGCCAAAAG TGAAGTTTCT CTTTGGAATC
TGGCCAGTGA TCCTGTTTGA GCCTCTCAGG AAGCATTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001287883.1
CDS461..979
Misc Feature(1)428..430(+)
Misc Feature(2)461..952(+)
Exon (1)1..207
Gene:ACER3
Gene Synonym:
Exon (2)208..318
Gene:ACER3
Gene Synonym:
Exon (3)319..442
Gene:ACER3
Gene Synonym:
Exon (4)443..495
Gene:ACER3
Gene Synonym:
Exon (5)496..577
Gene:ACER3
Gene Synonym:
Exon (6)578..613
Gene:ACER3
Gene Synonym:
Exon (7)614..672
Gene:ACER3
Gene Synonym:
Exon (8)673..774
Gene:ACER3
Gene Synonym:
Exon (9)775..879
Gene:ACER3
Gene Synonym:
Exon (10)880..925
Gene:ACER3
Gene Synonym:
Exon (11)926..7449
Gene:ACER3
Gene Synonym:
Translation
Position Chain Variation Link
13 13 a, g dbSNP:528589871
55 55 c, g dbSNP:754875567
60 60 c, t dbSNP:369558733
64 64 c, t dbSNP:778445091
83 83 -, ggc dbSNP:757425734
84 84 -, ggcggcggc dbSNP:765346972
84 84 -, ggc dbSNP:753848905
90 90 a, g dbSNP:747633542
92 92 c, t dbSNP:763435154
93 93 a, g dbSNP:374042980
102 102 a, g dbSNP:376920235
108 108 c, g dbSNP:777604778
114 114 a, g dbSNP:746227737
122 122 c, t dbSNP:770336801
128 128 c, g dbSNP:776004671
131 131 c, t dbSNP:749764911
139 139 a, g dbSNP:370824515
149 149 c, t dbSNP:138662843
184 184 a, t dbSNP:201190641
186 186 a, g dbSNP:774470717
187 187 a, c dbSNP:149293091
229 229 a, t dbSNP:773286511
240 240 c, t dbSNP:760844035
246 246 a, g dbSNP:754019271
251 251 c, t dbSNP:776464379
252 252 a, g, t dbSNP:372314534
258 258 a, g dbSNP:4379869
267 267 c, g dbSNP:762995174
274 274 a, g dbSNP:763660769
275 275 c, t dbSNP:751139978
276 276 a, g dbSNP:756951583
282 282 -, a dbSNP:781710875
282 282 c, g dbSNP:781035647
286 286 a, g dbSNP:555155221
287 287 a, g dbSNP:4479014
289 289 a, g dbSNP:755357196
294 294 a, g dbSNP:142469431
302 302 c, t dbSNP:748538912
306 306 c, t dbSNP:772596848
311 311 a, g dbSNP:777780701
313 313 c, t dbSNP:113648802
327 327 c, t dbSNP:548977140
334 334 a, g dbSNP:758828041
343 343 g, t dbSNP:764566631
344 344 c, t dbSNP:752001543
351 351 c, t dbSNP:757783921
375 375 a, g dbSNP:781089214
377 377 -, tc dbSNP:745564093
381 381 g, t dbSNP:746011232
452 452 a, g dbSNP:372226995
463 463 a, g, t dbSNP:201974871
464 464 a, t dbSNP:776323067
468 468 a, g dbSNP:772891253
478 478 c, t dbSNP:748773593
492 492 c, g dbSNP:759196304
495 495 c, t dbSNP:764372246
502 502 a, c dbSNP:777832951
516 516 a, g dbSNP:746486966
533 533 c, t dbSNP:79316237
547 547 c, t dbSNP:370822453
556 556 a, g dbSNP:759145370
565 565 a, g dbSNP:769455875
568 568 a, g dbSNP:774669854
574 574 a, c dbSNP:17417894
577 577 a, g dbSNP:145982324
601 601 a, c, g dbSNP:3740767
609 609 a, g dbSNP:761198154
651 651 a, g dbSNP:200663793
1117 1117 a, c dbSNP:12786211
1133 1133 a, t dbSNP:6592695
1811 1811 c, t dbSNP:767636567
1828 1828 a, g dbSNP:752779765
1916 1916 a, g dbSNP:7925774
1923 1923 g, t dbSNP:754024397
2052 2052 a, g dbSNP:113193324
2138 2138 c, t dbSNP:779767253
2354 2354 c, t dbSNP:591043
2392 2392 c, t dbSNP:1062170
2557 2557 c, t dbSNP:7123496
2632 2632 a, g dbSNP:10793231
2921 2921 a, c dbSNP:17135325
3438 3438 c, t dbSNP:144358416
3455 3455 c, t dbSNP:2826
3479 3479 c, t dbSNP:79522926
3530 3530 a, g dbSNP:200178452
3535 3535 a, c, g dbSNP:202111355
3613 3613 c, t dbSNP:4944134
3740 3740 g, t dbSNP:112256825
3859 3859 a, g dbSNP:7127936
3870 3870 a, g dbSNP:58717726
3937 3937 a, g dbSNP:7128038
4030 4030 a, g dbSNP:73491478
4453 4453 c, t dbSNP:6592696
4506 4506 a, c dbSNP:143377420
4522 4522 a, g dbSNP:61056520
4565 4565 a, c dbSNP:7950001
4580 4580 g, t dbSNP:141293002
4594 4594 c, g dbSNP:150227830
4656 4656 a, g dbSNP:12224200
4659 4659 a, g dbSNP:12786191
4665 4665 g, t dbSNP:12786192
4686 4686 a, c dbSNP:12788575
4692 4692 a, c dbSNP:12787874
4719 4719 c, t dbSNP:12787895
4726 4726 a, c dbSNP:12788596
4753 4753 -, a dbSNP:56406202
4755 4755 a, g dbSNP:12788609
4808 4808 a, g dbSNP:12363416
4824 4824 -, a dbSNP:57808641
4886 4886 a, g dbSNP:751366368
4922 4922 a, c dbSNP:199742172
4923 4923 a, t dbSNP:200335349
4924 4924 a, c dbSNP:201531878
4941 4941 -, aaaaaaaaaaaaa dbSNP:61767069
5086 5086 a, c dbSNP:76524197
5096 5096 a, g dbSNP:11237059
5132 5132 a, g dbSNP:11237060
5137 5137 a, g dbSNP:754778703
5302 5302 a, g dbSNP:78476665
5350 5350 c, t dbSNP:79463399
5371 5371 a, t dbSNP:10899344
6019 6019 g, t dbSNP:748950171
6064 6064 a, t dbSNP:1989463
6068 6068 a, t dbSNP:12801243
6730 6730 c, t dbSNP:111746566
6853 6853 a, g dbSNP:1945085
6914 6914 c, t dbSNP:199847342
6914 6914 -, t dbSNP:559748271
6915 6915 c, t dbSNP:200517484
7113 7113 a, t dbSNP:118100153

Target ORF information:

RefSeq Version NM_001300954
Organism Homo sapiens (human)
Definition Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001300954

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGATATACA GCTGTTGCAT ATTTGTGTAC TGCATGTTTG AATGTTTCAA GATCAAGAAC 
TCAGTAAACT ACCATCTGCT TTTTACCTTA GTTCTATTCA GTTTAATAGT AACCACAGTT
TACCTTAAGG TAAAAGAGCC GATATTCCAT CAGGTCATGT ATGGAATGTT GGTCTTTACA
TTAGTACTTC GATCTATTTA TATTGTTACA TGGGTTTATC CATGGCTTAG AGGACTGGGT
TATACATCAT TGGGTATATT TTTATTGGGA TTTTTATTTT GGAATATAGA TAACATATTT
TGTGAGTCAC TGAGGAACTT TCGAAAGAAG GTACCACCTA TCATAGGTAT TACCACACAA
TTTCATGCAT GGTGGCATAT TTTAACTGGC CTTGGTTCCT ATCTTCACAT CCTTTTCAGT
TTGTATACAA GAACACTTTA CCTGAGATAT AGGCCAAAAG TGAAGTTTCT CTTTGGAATC
TGGCCAGTGA TCCTGTTTGA GCCTCTCAGG AAGCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu45422
Accession Version NM_001300955.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product alkaline ceramidase 3 isoform c
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG702017.1, AK316120.1, AP000752.4 and AI885537.1. On Jul 17, 2014 this sequence version replaced gi:530396973. Transcript Variant: This variant (4) lacks an alternate exon and uses a downstream translation start codon compared to variant 1. The resulting isoform (c) is shorter at the N-terminus compared to isoform a. Variants 3 and 4 both encode the same isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK316120.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGATATACA GCTGTTGCAT ATTTGTGTAC TGCATGTTTG AATGTTTCAA GATCAAGAAC 
TCAGTAAACT ACCATCTGCT TTTTACCTTA GTTCTATTCA GTTTAATAGT AACCACAGTT
TACCTTAAGG TAAAAGAGCC GATATTCCAT CAGGTCATGT ATGGAATGTT GGTCTTTACA
TTAGTACTTC GATCTATTTA TATTGTTACA TGGGTTTATC CATGGCTTAG AGGACTGGGT
TATACATCAT TGGGTATATT TTTATTGGGA TTTTTATTTT GGAATATAGA TAACATATTT
TGTGAGTCAC TGAGGAACTT TCGAAAGAAG GTACCACCTA TCATAGGTAT TACCACACAA
TTTCATGCAT GGTGGCATAT TTTAACTGGC CTTGGTTCCT ATCTTCACAT CCTTTTCAGT
TTGTATACAA GAACACTTTA CCTGAGATAT AGGCCAAAAG TGAAGTTTCT CTTTGGAATC
TGGCCAGTGA TCCTGTTTGA GCCTCTCAGG AAGCATTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001287884.1
CDS337..855
Misc Feature(1)307..309(+)
Misc Feature(2)337..828(+)
Exon (1)1..207
Gene:ACER3
Gene Synonym:
Exon (2)208..318
Gene:ACER3
Gene Synonym:
Exon (3)319..371
Gene:ACER3
Gene Synonym:
Exon (4)372..453
Gene:ACER3
Gene Synonym:
Exon (5)454..489
Gene:ACER3
Gene Synonym:
Exon (6)490..548
Gene:ACER3
Gene Synonym:
Exon (7)549..650
Gene:ACER3
Gene Synonym:
Exon (8)651..755
Gene:ACER3
Gene Synonym:
Exon (9)756..801
Gene:ACER3
Gene Synonym:
Exon (10)802..7325
Gene:ACER3
Gene Synonym:
Translation
Position Chain Variation Link
13 13 a, g dbSNP:528589871
55 55 c, g dbSNP:754875567
60 60 c, t dbSNP:369558733
64 64 c, t dbSNP:778445091
83 83 -, ggc dbSNP:757425734
84 84 -, ggcggcggc dbSNP:765346972
84 84 -, ggc dbSNP:753848905
90 90 a, g dbSNP:747633542
92 92 c, t dbSNP:763435154
93 93 a, g dbSNP:374042980
102 102 a, g dbSNP:376920235
108 108 c, g dbSNP:777604778
114 114 a, g dbSNP:746227737
122 122 c, t dbSNP:770336801
128 128 c, g dbSNP:776004671
131 131 c, t dbSNP:749764911
139 139 a, g dbSNP:370824515
149 149 c, t dbSNP:138662843
184 184 a, t dbSNP:201190641
186 186 a, g dbSNP:774470717
187 187 a, c dbSNP:149293091
229 229 a, t dbSNP:773286511
240 240 c, t dbSNP:760844035
246 246 a, g dbSNP:754019271
251 251 c, t dbSNP:776464379
252 252 a, g, t dbSNP:372314534
258 258 a, g dbSNP:4379869
267 267 c, g dbSNP:762995174
274 274 a, g dbSNP:763660769
275 275 c, t dbSNP:751139978
276 276 a, g dbSNP:756951583
282 282 -, a dbSNP:781710875
282 282 c, g dbSNP:781035647
286 286 a, g dbSNP:555155221
287 287 a, g dbSNP:4479014
289 289 a, g dbSNP:755357196
294 294 a, g dbSNP:142469431
302 302 c, t dbSNP:748538912
306 306 c, t dbSNP:772596848
311 311 a, g dbSNP:777780701
313 313 c, t dbSNP:113648802
328 328 a, g dbSNP:372226995
339 339 a, g, t dbSNP:201974871
340 340 a, t dbSNP:776323067
344 344 a, g dbSNP:772891253
354 354 c, t dbSNP:748773593
368 368 c, g dbSNP:759196304
371 371 c, t dbSNP:764372246
378 378 a, c dbSNP:777832951
392 392 a, g dbSNP:746486966
409 409 c, t dbSNP:79316237
423 423 c, t dbSNP:370822453
432 432 a, g dbSNP:759145370
441 441 a, g dbSNP:769455875
444 444 a, g dbSNP:774669854
450 450 a, c dbSNP:17417894
453 453 a, g dbSNP:145982324
477 477 a, c, g dbSNP:3740767
485 485 a, g dbSNP:761198154
527 527 a, g dbSNP:200663793
993 993 a, c dbSNP:12786211
1009 1009 a, t dbSNP:6592695
1687 1687 c, t dbSNP:767636567
1704 1704 a, g dbSNP:752779765
1792 1792 a, g dbSNP:7925774
1799 1799 g, t dbSNP:754024397
1928 1928 a, g dbSNP:113193324
2014 2014 c, t dbSNP:779767253
2230 2230 c, t dbSNP:591043
2268 2268 c, t dbSNP:1062170
2433 2433 c, t dbSNP:7123496
2508 2508 a, g dbSNP:10793231
2797 2797 a, c dbSNP:17135325
3314 3314 c, t dbSNP:144358416
3331 3331 c, t dbSNP:2826
3355 3355 c, t dbSNP:79522926
3406 3406 a, g dbSNP:200178452
3411 3411 a, c, g dbSNP:202111355
3489 3489 c, t dbSNP:4944134
3616 3616 g, t dbSNP:112256825
3735 3735 a, g dbSNP:7127936
3746 3746 a, g dbSNP:58717726
3813 3813 a, g dbSNP:7128038
3906 3906 a, g dbSNP:73491478
4329 4329 c, t dbSNP:6592696
4382 4382 a, c dbSNP:143377420
4398 4398 a, g dbSNP:61056520
4441 4441 a, c dbSNP:7950001
4456 4456 g, t dbSNP:141293002
4470 4470 c, g dbSNP:150227830
4532 4532 a, g dbSNP:12224200
4535 4535 a, g dbSNP:12786191
4541 4541 g, t dbSNP:12786192
4562 4562 a, c dbSNP:12788575
4568 4568 a, c dbSNP:12787874
4595 4595 c, t dbSNP:12787895
4602 4602 a, c dbSNP:12788596
4629 4629 -, a dbSNP:56406202
4631 4631 a, g dbSNP:12788609
4684 4684 a, g dbSNP:12363416
4700 4700 -, a dbSNP:57808641
4762 4762 a, g dbSNP:751366368
4798 4798 a, c dbSNP:199742172
4799 4799 a, t dbSNP:200335349
4800 4800 a, c dbSNP:201531878
4817 4817 -, aaaaaaaaaaaaa dbSNP:61767069
4962 4962 a, c dbSNP:76524197
4972 4972 a, g dbSNP:11237059
5008 5008 a, g dbSNP:11237060
5013 5013 a, g dbSNP:754778703
5178 5178 a, g dbSNP:78476665
5226 5226 c, t dbSNP:79463399
5247 5247 a, t dbSNP:10899344
5895 5895 g, t dbSNP:748950171
5940 5940 a, t dbSNP:1989463
5944 5944 a, t dbSNP:12801243
6606 6606 c, t dbSNP:111746566
6729 6729 a, g dbSNP:1945085
6790 6790 c, t dbSNP:199847342
6790 6790 -, t dbSNP:559748271
6791 6791 c, t dbSNP:200517484
6989 6989 a, t dbSNP:118100153

Target ORF information:

RefSeq Version NM_001300955
Organism Homo sapiens (human)
Definition Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001300955

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGATATACA GCTGTTGCAT ATTTGTGTAC TGCATGTTTG AATGTTTCAA GATCAAGAAC 
TCAGTAAACT ACCATCTGCT TTTTACCTTA GTTCTATTCA GTTTAATAGT AACCACAGTT
TACCTTAAGG TAAAAGAGCC GATATTCCAT CAGGTCATGT ATGGAATGTT GGTCTTTACA
TTAGTACTTC GATCTATTTA TATTGTTACA TGGGTTTATC CATGGCTTAG AGGACTGGGT
TATACATCAT TGGGTATATT TTTATTGGGA TTTTTATTTT GGAATATAGA TAACATATTT
TGTGAGTCAC TGAGGAACTT TCGAAAGAAG GTACCACCTA TCATAGGTAT TACCACACAA
TTTCATGCAT GGTGGCATAT TTTAACTGGC CTTGGTTCCT ATCTTCACAT CCTTTTCAGT
TTGTATACAA GAACACTTTA CCTGAGATAT AGGCCAAAAG TGAAGTTTCT CTTTGGAATC
TGGCCAGTGA TCCTGTTTGA GCCTCTCAGG AAGCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu69362
Accession Version NM_001300953.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 693bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product alkaline ceramidase 3 isoform b
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG702017.1, AK294978.1, AP002498.3, AP000752.4 and AI885537.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK294978.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTCCGG CCGCGGACCG AGAGGGCTAC TGGGGCCCCA CGACCTCCAC GCTGGACTGG 
TGCGAGGAGA ACTACTCCGT GACCTGGTAC ATCGCCGAGT TCTTGGTAGG AATGGGATCC
TGGTGCTTCC ACATGACTCT GAAATATGAA ATGCAGCTAT TGGATGAACT CCCAATGATA
TACAGCTGTT GCATATTTGT GTACTGCATG TTTGAATGTT TCAAGATCAA GAACTCAGTA
AACTACCATC TGCTTTTTAC CTTAGTTCTA TTCAGTTTAA TAGTAACCAC AGTTTACCTT
AAGGTAAAAG AGCCGATATT CCATCAGGTC ATGTATGGAA TGTTGGTCTT TACATTAGTA
CTTCGATCTA TTTATATTGT TACATGGGTT TATCCATGGC TTAGAGGACT GGGTTATACA
TCATTGGGTA TATTTTTATT GGGATTTTTA TTTTGGAATA TAGATAACAT ATTTTGTGAG
TCACTGAGGA ACTTTCGAAA GAAGGTACCA CCTATCATAG GTATTACCAC ACAATTTCAT
GCATGGTGGC ATATTTTAAC TGGCCTTGGT TCCTATCTTC ACATCCTTTT CAGTTTGTAT
ACAAGAACAC TTTACCTGAG ATATAGGCCA AAAGTGAAGT TTCTCTTTGG AATCTGGCCA
GTGATCCTGT TTGAGCCTCT CAGGAAGCAT TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001287882.1
CDS105..797
Misc Feature(1)69..71(+)
Misc Feature(2)126..770(+)
Exon (1)1..207
Gene:ACER3
Gene Synonym:
Exon (2)208..260
Gene:ACER3
Gene Synonym:
Exon (3)261..313
Gene:ACER3
Gene Synonym:
Exon (4)314..395
Gene:ACER3
Gene Synonym:
Exon (5)396..431
Gene:ACER3
Gene Synonym:
Exon (6)432..490
Gene:ACER3
Gene Synonym:
Exon (7)491..592
Gene:ACER3
Gene Synonym:
Exon (8)593..697
Gene:ACER3
Gene Synonym:
Exon (9)698..743
Gene:ACER3
Gene Synonym:
Exon (10)744..7267
Gene:ACER3
Gene Synonym:
Translation
Position Chain Variation Link
13 13 a, g dbSNP:528589871
55 55 c, g dbSNP:754875567
60 60 c, t dbSNP:369558733
64 64 c, t dbSNP:778445091
83 83 -, ggc dbSNP:757425734
84 84 -, ggcggcggc dbSNP:765346972
84 84 -, ggc dbSNP:753848905
90 90 a, g dbSNP:747633542
92 92 c, t dbSNP:763435154
93 93 a, g dbSNP:374042980
102 102 a, g dbSNP:376920235
108 108 c, g dbSNP:777604778
114 114 a, g dbSNP:746227737
122 122 c, t dbSNP:770336801
128 128 c, g dbSNP:776004671
131 131 c, t dbSNP:749764911
139 139 a, g dbSNP:370824515
149 149 c, t dbSNP:138662843
184 184 a, t dbSNP:201190641
186 186 a, g dbSNP:774470717
187 187 a, c dbSNP:149293091
218 218 a, g dbSNP:530818806
236 236 c, t dbSNP:774489309
270 270 a, g dbSNP:372226995
281 281 a, g, t dbSNP:201974871
282 282 a, t dbSNP:776323067
286 286 a, g dbSNP:772891253
296 296 c, t dbSNP:748773593
310 310 c, g dbSNP:759196304
313 313 c, t dbSNP:764372246
320 320 a, c dbSNP:777832951
334 334 a, g dbSNP:746486966
351 351 c, t dbSNP:79316237
365 365 c, t dbSNP:370822453
374 374 a, g dbSNP:759145370
383 383 a, g dbSNP:769455875
386 386 a, g dbSNP:774669854
392 392 a, c dbSNP:17417894
395 395 a, g dbSNP:145982324
419 419 a, c, g dbSNP:3740767
427 427 a, g dbSNP:761198154
469 469 a, g dbSNP:200663793
935 935 a, c dbSNP:12786211
951 951 a, t dbSNP:6592695
1629 1629 c, t dbSNP:767636567
1646 1646 a, g dbSNP:752779765
1734 1734 a, g dbSNP:7925774
1741 1741 g, t dbSNP:754024397
1870 1870 a, g dbSNP:113193324
1956 1956 c, t dbSNP:779767253
2172 2172 c, t dbSNP:591043
2210 2210 c, t dbSNP:1062170
2375 2375 c, t dbSNP:7123496
2450 2450 a, g dbSNP:10793231
2739 2739 a, c dbSNP:17135325
3256 3256 c, t dbSNP:144358416
3273 3273 c, t dbSNP:2826
3297 3297 c, t dbSNP:79522926
3348 3348 a, g dbSNP:200178452
3353 3353 a, c, g dbSNP:202111355
3431 3431 c, t dbSNP:4944134
3558 3558 g, t dbSNP:112256825
3677 3677 a, g dbSNP:7127936
3688 3688 a, g dbSNP:58717726
3755 3755 a, g dbSNP:7128038
3848 3848 a, g dbSNP:73491478
4271 4271 c, t dbSNP:6592696
4324 4324 a, c dbSNP:143377420
4340 4340 a, g dbSNP:61056520
4383 4383 a, c dbSNP:7950001
4398 4398 g, t dbSNP:141293002
4412 4412 c, g dbSNP:150227830
4474 4474 a, g dbSNP:12224200
4477 4477 a, g dbSNP:12786191
4483 4483 g, t dbSNP:12786192
4504 4504 a, c dbSNP:12788575
4510 4510 a, c dbSNP:12787874
4537 4537 c, t dbSNP:12787895
4544 4544 a, c dbSNP:12788596
4571 4571 -, a dbSNP:56406202
4573 4573 a, g dbSNP:12788609
4626 4626 a, g dbSNP:12363416
4642 4642 -, a dbSNP:57808641
4704 4704 a, g dbSNP:751366368
4740 4740 a, c dbSNP:199742172
4741 4741 a, t dbSNP:200335349
4742 4742 a, c dbSNP:201531878
4759 4759 -, aaaaaaaaaaaaa dbSNP:61767069
4904 4904 a, c dbSNP:76524197
4914 4914 a, g dbSNP:11237059
4950 4950 a, g dbSNP:11237060
4955 4955 a, g dbSNP:754778703
5120 5120 a, g dbSNP:78476665
5168 5168 c, t dbSNP:79463399
5189 5189 a, t dbSNP:10899344
5837 5837 g, t dbSNP:748950171
5882 5882 a, t dbSNP:1989463
5886 5886 a, t dbSNP:12801243
6548 6548 c, t dbSNP:111746566
6671 6671 a, g dbSNP:1945085
6732 6732 c, t dbSNP:199847342
6732 6732 -, t dbSNP:559748271
6733 6733 c, t dbSNP:200517484
6931 6931 a, t dbSNP:118100153

Target ORF information:

RefSeq Version NM_001300953
Organism Homo sapiens (human)
Definition Homo sapiens alkaline ceramidase 3 (ACER3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001300953

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTCCGG CCGCGGACCG AGAGGGCTAC TGGGGCCCCA CGACCTCCAC GCTGGACTGG 
TGCGAGGAGA ACTACTCCGT GACCTGGTAC ATCGCCGAGT TCTTGGTAGG AATGGGATCC
TGGTGCTTCC ACATGACTCT GAAATATGAA ATGCAGCTAT TGGATGAACT CCCAATGATA
TACAGCTGTT GCATATTTGT GTACTGCATG TTTGAATGTT TCAAGATCAA GAACTCAGTA
AACTACCATC TGCTTTTTAC CTTAGTTCTA TTCAGTTTAA TAGTAACCAC AGTTTACCTT
AAGGTAAAAG AGCCGATATT CCATCAGGTC ATGTATGGAA TGTTGGTCTT TACATTAGTA
CTTCGATCTA TTTATATTGT TACATGGGTT TATCCATGGC TTAGAGGACT GGGTTATACA
TCATTGGGTA TATTTTTATT GGGATTTTTA TTTTGGAATA TAGATAACAT ATTTTGTGAG
TCACTGAGGA ACTTTCGAAA GAAGGTACCA CCTATCATAG GTATTACCAC ACAATTTCAT
GCATGGTGGC ATATTTTAAC TGGCCTTGGT TCCTATCTTC ACATCCTTTT CAGTTTGTAT
ACAAGAACAC TTTACCTGAG ATATAGGCCA AAAGTGAAGT TTCTCTTTGG AATCTGGCCA
GTGATCCTGT TTGAGCCTCT CAGGAAGCAT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.