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NOP10 cDNA ORF clone, Homo sapiens (human)

Gene Symbol NOP10
Entrez Gene ID 55505
Full Name NOP10 ribonucleoprotein
Synonyms DKCB1, NOLA3, NOP10P
General protein information
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

15

15q14-q15

Summary This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA1 and NOLA2 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. The four H/ACA snoRNP proteins are also components of the telomerase complex. This gene encodes a protein related to Saccharomyces cerevisiae Nop10p. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

606471

Disorder Html: Dyskeratosis congenita, autosomal recessive, 224230 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_018648 NP_061118 H/ACA ribonucleoprotein complex subunit 3

KEGG
hsa03008 Ribosome biogenesis in eukaryotes
hsa_M00425 H/ACA ribonucleoprotein complex


Homo sapiens (human) NOP10 NP_061118.1
Pan troglodytes (chimpanzee) NOP10 XP_001165824.1
Canis lupus familiaris (dog) NOP10 XP_849017.2
Bos taurus (cattle) NOP10 NP_001107993.1
Mus musculus (house mouse) Nop10 NP_079679.1
Rattus norvegicus (Norway rat) Nop10 NP_001119572.1
Danio rerio (zebrafish) nop10 NP_001003868.1
Drosophila melanogaster (fruit fly) CG7637 NP_610610.1
Xenopus (Silurana) tropicalis (western clawed frog) nop10 NP_001016163.1

Component

ID Name Evidence
GO:0005634 nucleus IEA
GO:0005730 nucleolus IEA
GO:0005732 small nucleolar ribonucleoprotein complex TAS
GO:0015030 Cajal body IEA

Function

ID Name Evidence
GO:0005515 protein binding IPI

Process

ID Name Evidence
GO:0001522 pseudouridine synthesis NAS
GO:0006364 rRNA processing IEA

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NOP10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOP10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19474
NM_018648 Homo sapiens NOP10 ribonucleoprotein (NOP10), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OHu19474
Accession Version NM_018648.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 195bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product H/ACA ribonucleoprotein complex subunit 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ218536.1 and BM554889.1. This sequence is a reference standard in the RefSeqGene project. On Oct 18, 2005 this sequence version replaced gi:15011920. Summary: This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA1 and NOLA2 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. The four H/ACA snoRNP proteins are also components of the telomerase complex. This gene encodes a protein related to Saccharomyces cerevisiae Nop10p. [provided by RefSeq, Jul 2008]. ##Evidence-Data-START## Transcript exon combination :: BU955288.1, AB043104.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
ATGTTTCTCC AGTATTACCT CAACGAGCAG GGAGATCGAG TCTATACGCT GAAGAAATTT 
GACCCGATGG GACAACAGAC CTGCTCAGCC CATCCTGCTC GGTTCTCCCC AGATGACAAA
TACTCTCGAC ACCGAATCAC CATCAAGAAA CGCTTCAAGG TGCTCATGAC CCAGCAACCG
CGCCCTGTCC TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_061118.1
CDS89..283
Misc Feature(1)80..82(+)
Misc Feature(2)95..247(+)
Misc Feature(3)194..196(+)
Exon (1)1..142
Gene:NOP10
Gene Synonym:
Exon (2)143..535
Gene:NOP10
Gene Synonym:
Translation
Position Chain Variation Link
15 15 c, t dbSNP:561915236
24 24 c, t dbSNP:550497412
30 30 c, g dbSNP:752462785
32 32 c, t dbSNP:775092898
38 38 a, g dbSNP:757349587
39 39 c, t dbSNP:554420284
42 42 c, t dbSNP:753733230
51 51 a, c, t dbSNP:112556317
56 56 a, g dbSNP:370148546
59 59 c, g, t dbSNP:201270793
66 66 a, g, t dbSNP:751282990
67 67 a, c, g dbSNP:762560152
68 68 a, c dbSNP:777218307
70 70 c, t dbSNP:201920207
71 71 a, c, g dbSNP:564679077
73 73 a, t dbSNP:764894891
75 75 a, g dbSNP:373689981
76 76 c, t dbSNP:772371219
79 79 a, g dbSNP:746040045
83 83 c, g dbSNP:779135425
84 84 c, t dbSNP:181783900
87 87 a, t dbSNP:749358405
94 94 -, t dbSNP:756134994
109 109 c, g dbSNP:777783319
112 112 c, t dbSNP:756047969
113 113 a, g dbSNP:752630312
114 114 a, g dbSNP:780995453
115 115 a, g dbSNP:373678744
119 119 a, g dbSNP:370894572
122 122 c, g dbSNP:146261631
126 126 c, g dbSNP:762749739
128 128 g, t dbSNP:749945545
129 129 g, t dbSNP:764689942
139 139 c, g dbSNP:761222362
140 140 a, c dbSNP:560253884
158 158 c, g dbSNP:773341436
163 163 a, t dbSNP:368082745
177 177 c, g dbSNP:748193565
180 180 a, c dbSNP:373843596
182 182 c, g dbSNP:768707296
188 188 c, t dbSNP:121908092
190 190 a, g dbSNP:746979721
200 200 a, g dbSNP:780315311
203 203 a, g dbSNP:779969910
210 210 a, g dbSNP:758289839
216 216 a, g dbSNP:745494123
222 222 a, g, t dbSNP:201604296
228 228 c, t dbSNP:756732260
238 238 a, g dbSNP:562412129
239 239 c, t dbSNP:763635922
240 240 a, g dbSNP:199661222
242 242 c, t dbSNP:752083783
246 246 a, g dbSNP:766693193
251 251 c, t dbSNP:763236137
263 263 a, c dbSNP:750603404
267 267 c, t dbSNP:765367178
274 274 c, t dbSNP:762158306
278 278 c, t dbSNP:776538895
285 285 c, g dbSNP:371001053
294 294 a, c dbSNP:768901406
302 302 a, t dbSNP:201041278
308 308 c, t dbSNP:377680031
313 313 ac, gt dbSNP:386782889
313 313 a, g dbSNP:1045194
314 314 c, t dbSNP:1045204
315 315 c, t dbSNP:370853886
316 316 c, t dbSNP:375648407
317 317 c, g dbSNP:770460479
323 323 c, t dbSNP:749018407
324 324 -, c dbSNP:528879821
326 326 c, t dbSNP:777217218
327 327 a, c, g dbSNP:372176417
328 328 c, g dbSNP:1045238
389 389 c, t dbSNP:146086719
402 402 c, t dbSNP:3062
406 406 c, t dbSNP:554752787
412 412 a, g dbSNP:115413508
419 419 a, c, t dbSNP:3063
432 432 a, g dbSNP:7173
433 433 a, g dbSNP:12818
437 437 g, t dbSNP:184964225
440 440 a, t dbSNP:15117
450 450 a, c dbSNP:753526616
452 452 c, t dbSNP:570903769
467 467 a, g, t dbSNP:113289404
474 474 c, t dbSNP:78931560
477 477 c, t dbSNP:766176686
484 484 c, t dbSNP:567004429
489 489 g, t dbSNP:760223677
499 499 c, t dbSNP:749924715
516 516 a, g, t dbSNP:762234908
522 522 c, g dbSNP:774993334
535 535 c, t dbSNP:548783054

Target ORF information:

RefSeq Version NM_018648
Organism Homo sapiens (human)
Definition Homo sapiens NOP10 ribonucleoprotein (NOP10), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_018648

ORF Insert Sequence:

1
61
121
181
ATGTTTCTCC AGTATTACCT CAACGAGCAG GGAGATCGAG TCTATACGCT GAAGAAATTT 
GACCCGATGG GACAACAGAC CTGCTCAGCC CATCCTGCTC GGTTCTCCCC AGATGACAAA
TACTCTCGAC ACCGAATCAC CATCAAGAAA CGCTTCAAGG TGCTCATGAC CCAGCAACCG
CGCCCTGTCC TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Effects of dyskeratosis congenita mutations in dyskerin, NHP2 and NOP10 on assembly of H/ACA pre-RNPs
Hum. Mol. Genet. 19 (5), 825-836 (2010)
Trahan C, Martel C and Dragon F.


book

Genetic heterogeneity in autosomal recessive dyskeratosis congenita with one subtype due to mutations in the telomerase-associated protein NOP10
Hum. Mol. Genet. 16 (13), 1619-1629 (2007)
Walne AJ, Vulliamy T, Marrone A, Beswick R, Kirwan M, Masunari Y, Al-Qurashi FH, Aljurf M and Dokal I.


book

hNaf1 is required for accumulation of human box H/ACA snoRNPs, scaRNPs, and telomerase
RNA 12 (5), 832-840 (2006)
Hoareau-Aveilla C, Bonoli M, Caizergues-Ferrer M and Henry Y.


book

Mutations in TERT, the gene for telomerase reverse transcriptase, in aplastic anemia
N. Engl. J. Med. 352 (14), 1413-1424 (2005)
Yamaguchi H, Calado RT, Ly H, Kajigaya S, Baerlocher GM, Chanock SJ, Lansdorp PM and Young NS.


book

Nucleolar proteome dynamics
Nature 433 (7021), 77-83 (2005)
Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI and Mann M.


book

Architecture and assembly of mammalian H/ACA small nucleolar and telomerase ribonucleoproteins
EMBO J. 23 (8), 1857-1867 (2004)
Wang C and Meier UT.


book

Human H/ACA small nucleolar RNPs and telomerase share evolutionarily conserved proteins NHP2 and NOP10
Mol. Cell. Biol. 20 (23), 9028-9040 (2000)
Pogacic V, Dragon F and Filipowicz W.


book

Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs
EMBO J. 17 (23), 7078-7090 (1998)
Henras A, Henry Y, Bousquet-Antonelli C, Noaillac-Depeyre J, Gelugne JP and Caizergues-Ferrer M.


book

Dyskeratosis Congenita
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Savage,S.A.


 
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