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PRF1 perforin 1 (pore forming protein) [Homo sapiens (human)]

Gene Symbol PRF1
Entrez Gene ID 5551
Full Name perforin 1 (pore forming protein)
Synonyms FLH2, HPLH2, P1, PFN1, PFP
General protein information
Preferred Names
perforin-1
Names
perforin-1
cytolysin
lymphocyte pore forming protein
lymphocyte pore-forming protein
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

10

10q22

Summary The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

170280

Disorder Html: Hemophagocytic lymphohistiocytosis, familial, 2, 603553 (3);
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu23295 NM_005041 Homo sapiens perforin 1 (pore forming protein) (PRF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu23295 NM_001083116 Homo sapiens perforin 1 (pore forming protein) (PRF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu23295D
Sequence Information ORF Nucleotide Sequence (Length: 1668bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product perforin-1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA816891.1, X13224.1, BC047695.1 and BQ654088.1. On Mar 21, 2007 this sequence version replaced gi:45935369. Summary: The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1) represents the shorter transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ555237.1, AK312754.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2150385, SAMEA2155751 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCAGCCC GTCTGCTCCT CCTGGGCATC CTTCTCCTGC TGCTGCCCCT GCCCGTCCCT 
GCCCCGTGCC ACACAGCCGC ACGCTCAGAG TGCAAGCGCA GCCACAAGTT CGTGCCTGGT
GCATGGCTGG CCGGGGAGGG TGTGGACGTG ACCAGCCTCC GCCGCTCGGG CTCCTTCCCA
GTGGACACAC AAAGGTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGTGA AAATGCCCTA
CAGGAGGGCA CCCTCCAGCG CCTGCCTCTG GCGCTCACCA ACTGGCGGGC CCAGGGCTCT
GGCTGCCAGC GCCATGTAAC CAGGGCCAAA GTCAGCTCCA CTGAAGCTGT GGCCCGGGAT
GCGGCTCGTA GCATCCGCAA CGACTGGAAG GTCGGGCTGG ACGTGACTCC TAAGCCCACC
AGCAATGTGC ATGTGTCTGT GGCCGGCTCA CACTCACAGG CAGCCAACTT TGCAGCCCAG
AAGACCCACC AGGACCAGTA CAGCTTCAGC ACTGACACGG TGGAGTGCCG CTTCTACAGT
TTCCATGTGG TACACACTCC CCCGCTGCAC CCTGACTTCA AGAGGGCCCT CGGGGACCTG
CCCCACCACT TCAACGCCTC CACCCAGCCC GCCTACCTCA GGCTTATCTC CAACTACGGC
ACCCACTTCA TCCGGGCTGT GGAGCTGGGT GGCCGCATAT CGGCCCTCAC TGCCCTGCGC
ACCTGCGAGC TGGCCCTGGA AGGGCTCACG GACAACGAGG TGGAGGACTG CCTGACTGTC
GAGGCCCAGG TCAACATAGG CATCCACGGC AGCATCTCTG CCGAAGCCAA GGCCTGTGAG
GAGAAGAAGA AGAAGCACAA GATGACGGCC TCCTTCCACC AAACCTACCG GGAGCGCCAC
TCGGAAGTGG TTGGCGGCCA TCACACCTCC ATTAACGACC TGCTGTTCGG GATCCAGGCC
GGGCCCGAGC AGTACTCAGC CTGGGTAAAC TCGCTGCCCG GCAGCCCTGG CCTGGTGGAC
TACACCCTGG AACCCCTGCA CGTGCTGCTG GACAGCCAGG ACCCGCGGCG GGAGGCACTG
AGGAGGGCCC TGAGTCAGTA CCTGACGGAC AGGGCTCGCT GGAGGGACTG CAGCCGGCCG
TGCCCACCAG GGCGGCAGAA GAGCCCCCGA GACCCATGCC AGTGTGTGTG CCATGGCTCA
GCGGTCACCA CCCAGGACTG CTGCCCTCGG CAGAGGGGCC TGGCCCAGCT GGAGGTGACC
TTCATCCAAG CATGGGGCCT GTGGGGGGAC TGGTTCACTG CCACGGATGC CTATGTGAAG
CTCTTCTTTG GTGGCCAGGA GCTGAGGACG AGCACCGTGT GGGACAATAA CAACCCCATC
TGGTCAGTGC GGCTGGATTT TGGGGATGTG CTCCTGGCCA CAGGGGGGCC CCTGAGGTTG
CAGGTCTGGG ATCAGGACTC TGGCAGGGAC GATGACCTCC TTGGCACCTG TGATCAGGCT
CCCAAGTCTG GTTCCCATGA GGTGAGATGC AACCTGAATC ATGGCCACCT AAAATTCCGC
TATCATGCCA GGTGCTTGCC CCACCTGGGA GGAGGCACCT GCCTGGACTA TGTCCCCCAA
ATGCTTCTGG GGGAGCCTCC AGGAAACCGG AGTGGGGCCG TGTGGTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_005032.2
CDS136..1803
Misc Feature(1)82..84(+)
Misc Feature(2)634..1242(+)
Misc Feature(3)775..777(+)
Misc Feature(4)1165..1167(+)
Misc Feature(5)1297..1677(+)
Misc Feature(6)1423..1611(+)
Misc Feature(7)748..756(+)
Exon (1)1..131
Gene:PRF1
Gene Synonym:
Exon (2)132..674
Gene:PRF1
Gene Synonym:
Exon (3)675..2508
Gene:PRF1
Gene Synonym:
Translation
Position Chain Variation Link
53 53 a, g dbSNP:114178404
83 83 a, g dbSNP:552823471
95 95 g, t dbSNP:749451125
110 110 a, g dbSNP:537825221
119 119 -, c dbSNP:35444577
138 138 -, t dbSNP:756032276
138 138 a, g dbSNP:758728749
141 141 a, g dbSNP:750631860
145 145 c, t dbSNP:12161733
146 146 a, g dbSNP:35418374
150 150 a, g dbSNP:752644970
154 154 c, t dbSNP:767449445
168 168 c, t dbSNP:759411132
171 171 -, cct dbSNP:750426581
182 182 c, t dbSNP:751379475
185 185 -, t dbSNP:147035858
187 187 a, c dbSNP:766121836
189 189 c, t dbSNP:762553478
190 190 a, g dbSNP:772896823
200 200 -, c dbSNP:761651233
200 200 c, t dbSNP:528937278
204 204 c, t dbSNP:761379626
209 209 c, g dbSNP:775934637
211 211 a, g dbSNP:769295756
212 212 c, t dbSNP:747466309
214 214 a, g dbSNP:147051158
215 215 c, t dbSNP:772544808
217 217 c, t dbSNP:141660796
224 224 a, c dbSNP:779192397
231 231 a, g dbSNP:2228018
232 232 a, c, t dbSNP:777782785
233 233 a, g dbSNP:531407289
240 240 a, c dbSNP:751467331
243 243 a, g dbSNP:766208450
246 246 c, t dbSNP:547321451
247 247 a, g dbSNP:201909472
267 267 a, c dbSNP:181323749
268 268 a, g dbSNP:578092914
282 282 a, c dbSNP:761310644
283 283 c, g dbSNP:776299562
285 285 a, g dbSNP:768253776
292 292 a, c dbSNP:761317587
293 293 a, t dbSNP:776129535
295 295 c, t dbSNP:200430442
296 296 a, g dbSNP:150357196
297 297 a, c dbSNP:779090799
298 298 c, t dbSNP:201032696
299 299 a, g dbSNP:140385034
303 303 a, g dbSNP:376059674
314 314 c, g dbSNP:756164148
315 315 a, g dbSNP:747004967
317 317 c, t dbSNP:779961315
320 320 -, acacacaaagg dbSNP:774071705
324 324 a, c dbSNP:759983785
325 325 c, t dbSNP:104894180
329 329 a, g dbSNP:750244443
337 337 a, c, t dbSNP:151232299
338 338 a, g dbSNP:753522684
341 341 c, t dbSNP:763759163
342 342 -, c dbSNP:786205093
343 343 a, g dbSNP:555317258
345 345 c, t dbSNP:202134601
346 346 a, g dbSNP:142059134
353 353 a, g dbSNP:759913385
356 356 c, t dbSNP:537165894
362 362 a, g dbSNP:776657932
375 375 a, t dbSNP:140752768
382 382 a, g dbSNP:773409738
383 383 a, g dbSNP:769928225
385 385 a, c dbSNP:748227721
394 394 c, t dbSNP:781070813
395 395 a, g dbSNP:557951406
407 407 c, t dbSNP:35947132
408 408 a, g dbSNP:778821122
409 409 c, t dbSNP:756992644
412 412 a, g dbSNP:753616524
416 416 a, c dbSNP:763847274
421 421 a, c, t dbSNP:144024245
422 422 a, g dbSNP:766899179
440 440 g, t dbSNP:760130891
444 444 c, g dbSNP:771525923
445 445 c, t dbSNP:547723649
446 446 a, g dbSNP:139225004
450 450 c, t dbSNP:763303548
452 452 g, t dbSNP:773497825
464 464 a, c dbSNP:770013555
476 476 c, t dbSNP:748315806
477 477 g, t dbSNP:776801201
484 484 a, g dbSNP:768584055
485 485 a, t dbSNP:745878717
486 486 g, t dbSNP:778714397
489 489 -, ccg dbSNP:764300792
490 490 c, g, t dbSNP:749110507
491 491 a, g dbSNP:777662073
495 495 c, t dbSNP:755864894
497 497 c, t dbSNP:752368313
498 498 a, g dbSNP:146358730
502 502 c, t dbSNP:370935432
503 503 a, c, g dbSNP:139336186
509 509 c, t dbSNP:763389567
511 511 c, t dbSNP:34279237
512 512 a, g dbSNP:765543157
515 515 a, g dbSNP:202091142
516 516 c, t dbSNP:531190813
517 517 a, g dbSNP:776708052
521 521 c, g dbSNP:768849283
525 525 c, g dbSNP:760679593
526 526 a, g, t dbSNP:761941942
528 528 a, c, t dbSNP:374948681
529 529 a, g dbSNP:201382038
533 533 c, t dbSNP:769690910
537 537 a, c, t dbSNP:564234285
538 538 a, g, t dbSNP:12263572
539 539 g, t dbSNP:200912683
543 543 a, t dbSNP:779486687
547 547 a, g dbSNP:201804583
554 554 a, c dbSNP:199885522
561 561 a, t dbSNP:750896540
566 566 a, g dbSNP:765636779
569 569 c, t dbSNP:535296791
570 570 a, g dbSNP:115281140
574 574 a, g dbSNP:764240753
578 578 a, c dbSNP:142012387
579 579 c, t dbSNP:61737403
580 580 a, g dbSNP:147462227
584 584 a, c, t dbSNP:773267292
587 587 a, t dbSNP:769775807
597 597 a, g dbSNP:116554195
600 600 c, t dbSNP:372962147
610 610 a, g dbSNP:768211924
611 611 c, g dbSNP:576356569
612 612 c, g dbSNP:139175805
622 622 c, t dbSNP:200712365
624 624 c, t dbSNP:143546173
634 634 g, t dbSNP:746443408
638 638 a, g dbSNP:779399414
642 642 c, t dbSNP:757728832
653 653 c, t dbSNP:754180700
654 654 a, c, g dbSNP:112883709
655 655 a, g dbSNP:752946887
663 663 c, t dbSNP:138588977
664 664 c, t dbSNP:201468340
665 665 a, g dbSNP:774503938
677 677 c, t dbSNP:770131011
680 680 a, g dbSNP:200383720
681 681 c, t dbSNP:781648443
683 683 g, t dbSNP:104894183
687 687 a, g dbSNP:755282048
691 691 a, g dbSNP:780802505
692 692 c, t dbSNP:751796432
694 694 c, t dbSNP:780042742
698 698 a, c, t dbSNP:150156593
705 705 a, c dbSNP:764108625
707 707 a, c dbSNP:778604519
717 717 c, g dbSNP:760559764
718 718 a, c dbSNP:752523306
726 726 c, t dbSNP:767273601
727 727 a, g dbSNP:759235360
731 731 a, t dbSNP:774041731
732 732 c, t dbSNP:770417198
739 739 c, t dbSNP:200332545
744 744 a, c dbSNP:772537976
749 749 a, g dbSNP:770354747
750 750 c, t dbSNP:141130900
751 751 a, g dbSNP:781717112
757 757 a, t dbSNP:769116034
759 759 c, t dbSNP:375086595
761 761 a, c dbSNP:146414576
765 765 c, t dbSNP:144004164
766 766 a, g dbSNP:762026835
767 767 c, t dbSNP:368524364
778 778 c, t dbSNP:778822630
792 792 a, c, t dbSNP:752608972
793 793 a, c, g dbSNP:776571416
794 794 a, g dbSNP:202217604
801 801 a, c dbSNP:751247865
805 805 a, g dbSNP:766068012
808 808 c, t dbSNP:28933973
809 809 a, g dbSNP:140281371
812 812 c, t dbSNP:764808366
817 817 a, g dbSNP:762455764
824 824 a, g dbSNP:753604724
828 828 c, t dbSNP:777261222
829 829 c, t dbSNP:550073023
830 830 a, g dbSNP:747380397
836 836 c, t dbSNP:775670991
837 837 a, g, t dbSNP:138508223
839 839 c, t dbSNP:142403457
844 844 a, g dbSNP:757315927
846 846 c, t dbSNP:748151937
852 852 g, t dbSNP:781114464
853 853 c, t dbSNP:754850577
854 854 a, g dbSNP:751390468
858 858 c, t dbSNP:766047301
861 861 c, t dbSNP:35329429
862 862 a, g dbSNP:766102404
870 870 c, g dbSNP:764894048
871 871 a, c dbSNP:552830871
873 873 a, g dbSNP:776211669
877 877 a, g dbSNP:764722375
878 878 g, t dbSNP:761049393
884 884 c, t dbSNP:775966864
885 885 a, g dbSNP:772306087
890 890 a, g dbSNP:28933375
891 891 c, t dbSNP:150053969
892 892 a, g dbSNP:771076819
900 900 g, t dbSNP:749385286
902 902 a, g dbSNP:777779022
910 910 a, g dbSNP:200368788
913 913 c, g dbSNP:746889280
915 915 c, t dbSNP:779948057
916 916 a, g dbSNP:758110629
925 925 a, g dbSNP:140218388
926 926 c, t dbSNP:764984064
931 931 a, g dbSNP:200824018
932 932 a, c, t dbSNP:763667216
941 941 a, g dbSNP:761293997
942 942 c, t dbSNP:147453010
943 943 a, g dbSNP:143043887
956 956 a, c, t dbSNP:747145070
957 957 c, t dbSNP:885821
959 959 a, c dbSNP:763119215
966 966 c, g dbSNP:773317614
971 971 a, g dbSNP:104894182
985 985 a, g dbSNP:199962500
986 986 a, g dbSNP:200716028
987 987 g, t dbSNP:747025790
988 988 -, aag dbSNP:745902829
988 988 a, c dbSNP:753226704
1001 1001 c, t dbSNP:150628656
1002 1002 a, g dbSNP:745670464
1005 1005 c, g, t dbSNP:376506267
1008 1008 c, t dbSNP:149008090
1020 1020 c, t dbSNP:757011029
1021 1021 c, t dbSNP:148237800
1023 1023 c, t dbSNP:777483971
1024 1024 c, t dbSNP:755578676
1025 1025 a, g dbSNP:752189303
1026 1026 a, g dbSNP:576966880
1029 1029 a, g dbSNP:760042830
1034 1034 a, g dbSNP:751950203
1035 1035 c, t dbSNP:885822
1038 1038 a, g dbSNP:763207236
1043 1043 a, t dbSNP:773405626
1050 1050 c, t dbSNP:769982593
1051 1051 a, g dbSNP:763002067
1056 1056 -, t dbSNP:777345151
1058 1058 a, t dbSNP:776713460
1059 1059 c, t dbSNP:771923778
1060 1060 a, g dbSNP:745770923
1061 1061 c, t dbSNP:754196056
1062 1062 c, t dbSNP:142643493
1065 1065 c, t dbSNP:770837333
1071 1071 c, t dbSNP:749015711
1072 1072 a, g dbSNP:777299577
1073 1073 a, t dbSNP:755737064
1074 1074 c, t dbSNP:752174520
1080 1080 a, g dbSNP:138159445
1083 1083 a, c, g, t dbSNP:201235771
1084 1084 a, g dbSNP:766783280
1086 1086 a, g dbSNP:201565901
1089 1089 c, t dbSNP:750718842
1092 1092 a, g dbSNP:554116546
1095 1095 c, t dbSNP:762044141
1096 1096 a, g dbSNP:145234214
1101 1101 c, t dbSNP:535958677
1102 1102 a, g dbSNP:375522165
1104 1104 a, g dbSNP:774426204
1107 1107 g, t dbSNP:770929453
1115 1115 c, t dbSNP:371021342
1120 1120 a, g dbSNP:749112632
1125 1125 c, t dbSNP:368769599
1127 1127 c, t dbSNP:769364825
1128 1128 a, g dbSNP:747803356
1134 1134 c, t dbSNP:140260618
1135 1135 a, c, g dbSNP:145463632
1136 1136 a, g dbSNP:780544163
1137 1137 c, g dbSNP:564568406
1140 1140 a, c dbSNP:758844674
1146 1146 a, c dbSNP:750774864
1149 1149 a, g dbSNP:765594736
1152 1152 a, g dbSNP:757472177
1153 1153 a, g dbSNP:754079962
1168 1168 a, c, t dbSNP:200641752
1169 1169 a, c, t dbSNP:28933374
1171 1171 c, t dbSNP:374261838
1173 1173 c, g dbSNP:762946719
1174 1174 c, t dbSNP:556996220
1176 1176 c, t dbSNP:150104693
1177 1177 a, c, g dbSNP:776338709
1182 1182 a, g, t dbSNP:771218500
1199 1199 c, t dbSNP:779488735
1200 1200 a, g dbSNP:758932748
1201 1201 c, t dbSNP:746365230
1202 1202 a, g dbSNP:571015630
1204 1204 c, t dbSNP:757636984
1205 1205 a, g dbSNP:140787739
1213 1213 c, t dbSNP:151083232
1215 1215 a, g dbSNP:756358874
1216 1216 a, t dbSNP:752858869
1225 1225 -, ct dbSNP:771552960
1233 1233 a, g dbSNP:767676771
1241 1241 c, t dbSNP:201584464
1242 1242 a, g dbSNP:145589960
1246 1246 a, g dbSNP:566891566
1247 1247 a, g dbSNP:199522390
1252 1252 c, g, t dbSNP:374588624
1253 1253 a, g dbSNP:140179771
1254 1254 a, c dbSNP:370384696
1257 1257 a, g dbSNP:104894176
1269 1269 a, c dbSNP:771559242
1271 1271 a, g dbSNP:746431351
1274 1274 c, g, t dbSNP:530097547
1275 1275 a, g dbSNP:144926997
1279 1279 c, t dbSNP:377367451
1280 1280 c, t dbSNP:756446764
1288 1288 c, t dbSNP:551046401
1289 1289 a, g dbSNP:781476866
1290 1290 a, g, t dbSNP:751523638
1291 1291 c, t dbSNP:765232909
1298 1298 g, t dbSNP:193302875
1299 1299 c, t dbSNP:761607073
1300 1300 c, t dbSNP:376289273
1302 1302 a, c dbSNP:763844396
1303 1303 c, t dbSNP:760379846
1304 1304 a, g dbSNP:775121659
1307 1307 a, t dbSNP:771647131
1309 1309 a, c dbSNP:759100720
1323 1323 a, g dbSNP:773688959
1329 1329 g, t dbSNP:148706255
1331 1331 g, t dbSNP:749729753
1334 1334 c, t dbSNP:778335116
1337 1337 c, t dbSNP:144242324
1338 1338 a, g dbSNP:748445504
1341 1341 c, t dbSNP:149581266
1342 1342 a, g dbSNP:371232368
1343 1343 c, t dbSNP:755093665
1346 1346 a, c, t dbSNP:779956870
1347 1347 c, t dbSNP:757256300
1354 1354 g, t dbSNP:753751595
1363 1363 c, t dbSNP:139322149
1364 1364 a, g dbSNP:150558419
1375 1375 c, g dbSNP:752431340
1379 1379 a, c, g dbSNP:759074628
1381 1381 c, t dbSNP:193302876
1384 1384 c, t dbSNP:765787505
1389 1389 a, c, g dbSNP:200789852
1394 1394 a, c dbSNP:770355235
1396 1396 c, t dbSNP:202216158
1397 1397 g, t dbSNP:200534908
1402 1402 c, t dbSNP:768953378
1409 1409 a, g dbSNP:142606828
1411 1411 a, g dbSNP:1042652
1418 1418 -, tgg dbSNP:747392486
1418 1418 g, t dbSNP:747646127
1421 1421 a, g dbSNP:104894181
1422 1422 a, g dbSNP:780229927
1431 1431 c, g dbSNP:752261431
1439 1439 c, t dbSNP:28933376
1440 1440 a, g dbSNP:200167080
1445 1445 c, t dbSNP:138126912
1447 1447 c, t dbSNP:755998254
1448 1448 a, g dbSNP:752523287
1452 1452 a, g dbSNP:767189454
1465 1465 -, ggtggc dbSNP:778216917
1469 1469 a, g dbSNP:754711256
1472 1472 a, c dbSNP:751161742
1474 1474 c, g dbSNP:145513232
1484 1484 c, t dbSNP:189650890
1485 1485 a, g dbSNP:200312496
1488 1488 c, t dbSNP:772785941
1491 1491 c, t dbSNP:765858178
1492 1492 a, g dbSNP:141864315
1500 1500 a, c dbSNP:777094015
1501 1501 a, c dbSNP:768878385
1508 1508 a, c dbSNP:528511999
1512 1512 a, c, t dbSNP:2228019
1513 1513 a, g dbSNP:772281903
1524 1524 a, g dbSNP:745970558
1525 1525 a, c, t dbSNP:754602851
1526 1526 a, g dbSNP:748060067
1541 1541 a, g dbSNP:577367079
1546 1546 c, t dbSNP:143040535
1555 1555 a, g dbSNP:751298400
1559 1559 a, g dbSNP:779584225
1562 1562 a, g, t dbSNP:377665096
1563 1563 -, g dbSNP:754622171
1563 1563 a, g dbSNP:145695221
1563 1563 -, g dbSNP:748926167
1565 1565 c, t dbSNP:762457806
1566 1566 c, t dbSNP:754285204
1567 1567 -, c dbSNP:779553489
1577 1577 a, c dbSNP:764596094
1579 1579 g, t dbSNP:760962796
1585 1585 a, g dbSNP:775875013
1586 1586 -, a dbSNP:755693770
1592 1592 a, c dbSNP:772313460
1605 1605 c, t dbSNP:759727585
1608 1608 c, t dbSNP:144968603
1613 1613 c, t dbSNP:774467483
1622 1622 c, t dbSNP:770987257
1634 1634 c, t dbSNP:375473178
1635 1635 a, t dbSNP:748186368
1636 1636 c, t dbSNP:200589152
1645 1645 c, g dbSNP:768577508
1653 1653 a, t dbSNP:746797570
1654 1654 g, t dbSNP:779855980
1656 1656 g, t dbSNP:200015777
1673 1673 -, aa dbSNP:749918235
1676 1676 a, g dbSNP:758163148
1693 1693 c, t dbSNP:377162454
1694 1694 a, g dbSNP:567204132
1695 1695 c, t dbSNP:147087107
1709 1709 a, g dbSNP:141702424
1718 1718 a, c dbSNP:753332095
1745 1745 a, g dbSNP:536292356
1751 1751 a, c dbSNP:764531237
1753 1753 a, c dbSNP:761206162
1755 1755 a, g dbSNP:149776121
1764 1764 g, t dbSNP:767848717
1771 1771 c, g dbSNP:550799905
1774 1774 a, c, g, t dbSNP:372713247
1778 1778 a, g dbSNP:144892262
1794 1794 c, t dbSNP:773289332
1795 1795 g, t dbSNP:768505698
1797 1797 a, g dbSNP:746931159
1812 1812 a, g dbSNP:779858353
1829 1829 c, t dbSNP:755876007
1834 1834 a, t dbSNP:376512084
1840 1840 a, t dbSNP:532610868
1841 1841 a, g dbSNP:571369275
1847 1847 a, g dbSNP:745582641
1849 1849 c, t dbSNP:140956842
1892 1892 c, t dbSNP:6480459
1898 1898 c, t dbSNP:560894722
1899 1899 a, g dbSNP:1889490
1907 1907 c, t dbSNP:752753581
1920 1920 c, t dbSNP:146571282
1926 1926 a, g dbSNP:563246193
1940 1940 g, t dbSNP:184146097
1942 1942 a, c, t dbSNP:56743633
1977 1977 c, t dbSNP:74513535
1979 1979 a, g dbSNP:573468737
1997 1997 -, ttt dbSNP:367570230
1998 1998 a, t dbSNP:79084177
2011 2011 -, t dbSNP:527442892
2012 2012 -, t dbSNP:34914326
2076 2076 a, g dbSNP:762398949
2169 2169 c, t dbSNP:139298157
2170 2170 c, g dbSNP:146245380
2179 2179 a, g dbSNP:191816002
2196 2196 c, t dbSNP:557290848
2211 2211 a, g dbSNP:143433002
2244 2244 a, g dbSNP:375066000
2269 2269 a, g dbSNP:546684854
2283 2283 a, g dbSNP:766663557
2284 2284 a, g dbSNP:187368446
2285 2285 c, t dbSNP:183113813
2308 2308 a, g dbSNP:368777270
2321 2321 g, t dbSNP:377317822
2324 2324 a, c dbSNP:193221010
2362 2362 g, t dbSNP:530803679
2408 2408 g, t dbSNP:563151587
2488 2488 a, g dbSNP:551388704

Target ORF information:

RefSeq Version NM_005041
Organism Homo sapiens (human)
Definition Homo sapiens perforin 1 (pore forming protein) (PRF1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_005041

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCAGCCC GTCTGCTCCT CCTGGGCATC CTTCTCCTGC TGCTGCCCCT GCCCGTCCCT 
GCCCCGTGCC ACACAGCCGC ACGCTCAGAG TGCAAGCGCA GCCACAAGTT CGTGCCTGGT
GCATGGCTGG CCGGGGAGGG TGTGGACGTG ACCAGCCTCC GCCGCTCGGG CTCCTTCCCA
GTGGACACAC AAAGGTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGTGA AAATGCCCTA
CAGGAGGGCA CCCTCCAGCG CCTGCCTCTG GCGCTCACCA ACTGGCGGGC CCAGGGCTCT
GGCTGCCAGC GCCATGTAAC CAGGGCCAAA GTCAGCTCCA CTGAAGCTGT GGCCCGGGAT
GCGGCTCGTA GCATCCGCAA CGACTGGAAG GTCGGGCTGG ACGTGACTCC TAAGCCCACC
AGCAATGTGC ATGTGTCTGT GGCCGGCTCA CACTCACAGG CAGCCAACTT TGCAGCCCAG
AAGACCCACC AGGACCAGTA CAGCTTCAGC ACTGACACGG TGGAGTGCCG CTTCTACAGT
TTCCATGTGG TACACACTCC CCCGCTGCAC CCTGACTTCA AGAGGGCCCT CGGGGACCTG
CCCCACCACT TCAACGCCTC CACCCAGCCC GCCTACCTCA GGCTTATCTC CAACTACGGC
ACCCACTTCA TCCGGGCTGT GGAGCTGGGT GGCCGCATAT CGGCCCTCAC TGCCCTGCGC
ACCTGCGAGC TGGCCCTGGA AGGGCTCACG GACAACGAGG TGGAGGACTG CCTGACTGTC
GAGGCCCAGG TCAACATAGG CATCCACGGC AGCATCTCTG CCGAAGCCAA GGCCTGTGAG
GAGAAGAAGA AGAAGCACAA GATGACGGCC TCCTTCCACC AAACCTACCG GGAGCGCCAC
TCGGAAGTGG TTGGCGGCCA TCACACCTCC ATTAACGACC TGCTGTTCGG GATCCAGGCC
GGGCCCGAGC AGTACTCAGC CTGGGTAAAC TCGCTGCCCG GCAGCCCTGG CCTGGTGGAC
TACACCCTGG AACCCCTGCA CGTGCTGCTG GACAGCCAGG ACCCGCGGCG GGAGGCACTG
AGGAGGGCCC TGAGTCAGTA CCTGACGGAC AGGGCTCGCT GGAGGGACTG CAGCCGGCCG
TGCCCACCAG GGCGGCAGAA GAGCCCCCGA GACCCATGCC AGTGTGTGTG CCATGGCTCA
GCGGTCACCA CCCAGGACTG CTGCCCTCGG CAGAGGGGCC TGGCCCAGCT GGAGGTGACC
TTCATCCAAG CATGGGGCCT GTGGGGGGAC TGGTTCACTG CCACGGATGC CTATGTGAAG
CTCTTCTTTG GTGGCCAGGA GCTGAGGACG AGCACCGTGT GGGACAATAA CAACCCCATC
TGGTCAGTGC GGCTGGATTT TGGGGATGTG CTCCTGGCCA CAGGGGGGCC CCTGAGGTTG
CAGGTCTGGG ATCAGGACTC TGGCAGGGAC GATGACCTCC TTGGCACCTG TGATCAGGCT
CCCAAGTCTG GTTCCCATGA GGTGAGATGC AACCTGAATC ATGGCCACCT AAAATTCCGC
TATCATGCCA GGTGCTTGCC CCACCTGGGA GGAGGCACCT GCCTGGACTA TGTCCCCCAA
ATGCTTCTGG GGGAGCCTCC AGGAAACCGG AGTGGGGCCG TGTGGTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu23295D
Sequence Information ORF Nucleotide Sequence (Length: 1668bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product perforin-1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA941244.1, X13224.1, BC047695.1 and BQ654088.1. This sequence is a reference standard in the RefSeqGene project. Summary: The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: FJ555237.1, AK312754.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2155751 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCAGCCC GTCTGCTCCT CCTGGGCATC CTTCTCCTGC TGCTGCCCCT GCCCGTCCCT 
GCCCCGTGCC ACACAGCCGC ACGCTCAGAG TGCAAGCGCA GCCACAAGTT CGTGCCTGGT
GCATGGCTGG CCGGGGAGGG TGTGGACGTG ACCAGCCTCC GCCGCTCGGG CTCCTTCCCA
GTGGACACAC AAAGGTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGTGA AAATGCCCTA
CAGGAGGGCA CCCTCCAGCG CCTGCCTCTG GCGCTCACCA ACTGGCGGGC CCAGGGCTCT
GGCTGCCAGC GCCATGTAAC CAGGGCCAAA GTCAGCTCCA CTGAAGCTGT GGCCCGGGAT
GCGGCTCGTA GCATCCGCAA CGACTGGAAG GTCGGGCTGG ACGTGACTCC TAAGCCCACC
AGCAATGTGC ATGTGTCTGT GGCCGGCTCA CACTCACAGG CAGCCAACTT TGCAGCCCAG
AAGACCCACC AGGACCAGTA CAGCTTCAGC ACTGACACGG TGGAGTGCCG CTTCTACAGT
TTCCATGTGG TACACACTCC CCCGCTGCAC CCTGACTTCA AGAGGGCCCT CGGGGACCTG
CCCCACCACT TCAACGCCTC CACCCAGCCC GCCTACCTCA GGCTTATCTC CAACTACGGC
ACCCACTTCA TCCGGGCTGT GGAGCTGGGT GGCCGCATAT CGGCCCTCAC TGCCCTGCGC
ACCTGCGAGC TGGCCCTGGA AGGGCTCACG GACAACGAGG TGGAGGACTG CCTGACTGTC
GAGGCCCAGG TCAACATAGG CATCCACGGC AGCATCTCTG CCGAAGCCAA GGCCTGTGAG
GAGAAGAAGA AGAAGCACAA GATGACGGCC TCCTTCCACC AAACCTACCG GGAGCGCCAC
TCGGAAGTGG TTGGCGGCCA TCACACCTCC ATTAACGACC TGCTGTTCGG GATCCAGGCC
GGGCCCGAGC AGTACTCAGC CTGGGTAAAC TCGCTGCCCG GCAGCCCTGG CCTGGTGGAC
TACACCCTGG AACCCCTGCA CGTGCTGCTG GACAGCCAGG ACCCGCGGCG GGAGGCACTG
AGGAGGGCCC TGAGTCAGTA CCTGACGGAC AGGGCTCGCT GGAGGGACTG CAGCCGGCCG
TGCCCACCAG GGCGGCAGAA GAGCCCCCGA GACCCATGCC AGTGTGTGTG CCATGGCTCA
GCGGTCACCA CCCAGGACTG CTGCCCTCGG CAGAGGGGCC TGGCCCAGCT GGAGGTGACC
TTCATCCAAG CATGGGGCCT GTGGGGGGAC TGGTTCACTG CCACGGATGC CTATGTGAAG
CTCTTCTTTG GTGGCCAGGA GCTGAGGACG AGCACCGTGT GGGACAATAA CAACCCCATC
TGGTCAGTGC GGCTGGATTT TGGGGATGTG CTCCTGGCCA CAGGGGGGCC CCTGAGGTTG
CAGGTCTGGG ATCAGGACTC TGGCAGGGAC GATGACCTCC TTGGCACCTG TGATCAGGCT
CCCAAGTCTG GTTCCCATGA GGTGAGATGC AACCTGAATC ATGGCCACCT AAAATTCCGC
TATCATGCCA GGTGCTTGCC CCACCTGGGA GGAGGCACCT GCCTGGACTA TGTCCCCCAA
ATGCTTCTGG GGGAGCCTCC AGGAAACCGG AGTGGGGCCG TGTGGTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001076585.1
CDS162..1829
Misc Feature(1)105..107(+)
Misc Feature(2)660..1268(+)
Misc Feature(3)801..803(+)
Misc Feature(4)1191..1193(+)
Misc Feature(5)1323..1703(+)
Misc Feature(6)1449..1637(+)
Misc Feature(7)774..782(+)
Exon (1)1..131
Gene:PRF1
Gene Synonym:
Exon (2)132..700
Gene:PRF1
Gene Synonym:
Exon (3)701..2534
Gene:PRF1
Gene Synonym:
Translation
Position Chain Variation Link
53 53 a, g dbSNP:114178404
83 83 a, g dbSNP:552823471
95 95 g, t dbSNP:749451125
110 110 a, g dbSNP:537825221
119 119 -, c dbSNP:35444577
146 146 -, ct dbSNP:765877069
164 164 -, t dbSNP:756032276
164 164 a, g dbSNP:758728749
167 167 a, g dbSNP:750631860
171 171 c, t dbSNP:12161733
172 172 a, g dbSNP:35418374
176 176 a, g dbSNP:752644970
180 180 c, t dbSNP:767449445
194 194 c, t dbSNP:759411132
197 197 -, cct dbSNP:750426581
208 208 c, t dbSNP:751379475
211 211 -, t dbSNP:147035858
213 213 a, c dbSNP:766121836
215 215 c, t dbSNP:762553478
216 216 a, g dbSNP:772896823
226 226 -, c dbSNP:761651233
226 226 c, t dbSNP:528937278
230 230 c, t dbSNP:761379626
235 235 c, g dbSNP:775934637
237 237 a, g dbSNP:769295756
238 238 c, t dbSNP:747466309
240 240 a, g dbSNP:147051158
241 241 c, t dbSNP:772544808
243 243 c, t dbSNP:141660796
250 250 a, c dbSNP:779192397
257 257 a, g dbSNP:2228018
258 258 a, c, t dbSNP:777782785
259 259 a, g dbSNP:531407289
266 266 a, c dbSNP:751467331
269 269 a, g dbSNP:766208450
272 272 c, t dbSNP:547321451
273 273 a, g dbSNP:201909472
293 293 a, c dbSNP:181323749
294 294 a, g dbSNP:578092914
308 308 a, c dbSNP:761310644
309 309 c, g dbSNP:776299562
311 311 a, g dbSNP:768253776
318 318 a, c dbSNP:761317587
319 319 a, t dbSNP:776129535
321 321 c, t dbSNP:200430442
322 322 a, g dbSNP:150357196
323 323 a, c dbSNP:779090799
324 324 c, t dbSNP:201032696
325 325 a, g dbSNP:140385034
329 329 a, g dbSNP:376059674
340 340 c, g dbSNP:756164148
341 341 a, g dbSNP:747004967
343 343 c, t dbSNP:779961315
346 346 -, acacacaaagg dbSNP:774071705
350 350 a, c dbSNP:759983785
351 351 c, t dbSNP:104894180
355 355 a, g dbSNP:750244443
363 363 a, c, t dbSNP:151232299
364 364 a, g dbSNP:753522684
367 367 c, t dbSNP:763759163
368 368 -, c dbSNP:786205093
369 369 a, g dbSNP:555317258
371 371 c, t dbSNP:202134601
372 372 a, g dbSNP:142059134
379 379 a, g dbSNP:759913385
382 382 c, t dbSNP:537165894
388 388 a, g dbSNP:776657932
401 401 a, t dbSNP:140752768
408 408 a, g dbSNP:773409738
409 409 a, g dbSNP:769928225
411 411 a, c dbSNP:748227721
420 420 c, t dbSNP:781070813
421 421 a, g dbSNP:557951406
433 433 c, t dbSNP:35947132
434 434 a, g dbSNP:778821122
435 435 c, t dbSNP:756992644
438 438 a, g dbSNP:753616524
442 442 a, c dbSNP:763847274
447 447 a, c, t dbSNP:144024245
448 448 a, g dbSNP:766899179
466 466 g, t dbSNP:760130891
470 470 c, g dbSNP:771525923
471 471 c, t dbSNP:547723649
472 472 a, g dbSNP:139225004
476 476 c, t dbSNP:763303548
478 478 g, t dbSNP:773497825
490 490 a, c dbSNP:770013555
502 502 c, t dbSNP:748315806
503 503 g, t dbSNP:776801201
510 510 a, g dbSNP:768584055
511 511 a, t dbSNP:745878717
512 512 g, t dbSNP:778714397
515 515 -, ccg dbSNP:764300792
516 516 c, g, t dbSNP:749110507
517 517 a, g dbSNP:777662073
521 521 c, t dbSNP:755864894
523 523 c, t dbSNP:752368313
524 524 a, g dbSNP:146358730
528 528 c, t dbSNP:370935432
529 529 a, c, g dbSNP:139336186
535 535 c, t dbSNP:763389567
537 537 c, t dbSNP:34279237
538 538 a, g dbSNP:765543157
541 541 a, g dbSNP:202091142
542 542 c, t dbSNP:531190813
543 543 a, g dbSNP:776708052
547 547 c, g dbSNP:768849283
551 551 c, g dbSNP:760679593
552 552 a, g, t dbSNP:761941942
554 554 a, c, t dbSNP:374948681
555 555 a, g dbSNP:201382038
559 559 c, t dbSNP:769690910
563 563 a, c, t dbSNP:564234285
564 564 a, g, t dbSNP:12263572
565 565 g, t dbSNP:200912683
569 569 a, t dbSNP:779486687
573 573 a, g dbSNP:201804583
580 580 a, c dbSNP:199885522
587 587 a, t dbSNP:750896540
592 592 a, g dbSNP:765636779
595 595 c, t dbSNP:535296791
596 596 a, g dbSNP:115281140
600 600 a, g dbSNP:764240753
604 604 a, c dbSNP:142012387
605 605 c, t dbSNP:61737403
606 606 a, g dbSNP:147462227
610 610 a, c, t dbSNP:773267292
613 613 a, t dbSNP:769775807
623 623 a, g dbSNP:116554195
626 626 c, t dbSNP:372962147
636 636 a, g dbSNP:768211924
637 637 c, g dbSNP:576356569
638 638 c, g dbSNP:139175805
648 648 c, t dbSNP:200712365
650 650 c, t dbSNP:143546173
660 660 g, t dbSNP:746443408
664 664 a, g dbSNP:779399414
668 668 c, t dbSNP:757728832
679 679 c, t dbSNP:754180700
680 680 a, c, g dbSNP:112883709
681 681 a, g dbSNP:752946887
689 689 c, t dbSNP:138588977
690 690 c, t dbSNP:201468340
691 691 a, g dbSNP:774503938
703 703 c, t dbSNP:770131011
706 706 a, g dbSNP:200383720
707 707 c, t dbSNP:781648443
709 709 g, t dbSNP:104894183
713 713 a, g dbSNP:755282048
717 717 a, g dbSNP:780802505
718 718 c, t dbSNP:751796432
720 720 c, t dbSNP:780042742
724 724 a, c, t dbSNP:150156593
731 731 a, c dbSNP:764108625
733 733 a, c dbSNP:778604519
743 743 c, g dbSNP:760559764
744 744 a, c dbSNP:752523306
752 752 c, t dbSNP:767273601
753 753 a, g dbSNP:759235360
757 757 a, t dbSNP:774041731
758 758 c, t dbSNP:770417198
765 765 c, t dbSNP:200332545
770 770 a, c dbSNP:772537976
775 775 a, g dbSNP:770354747
776 776 c, t dbSNP:141130900
777 777 a, g dbSNP:781717112
783 783 a, t dbSNP:769116034
785 785 c, t dbSNP:375086595
787 787 a, c dbSNP:146414576
791 791 c, t dbSNP:144004164
792 792 a, g dbSNP:762026835
793 793 c, t dbSNP:368524364
804 804 c, t dbSNP:778822630
818 818 a, c, t dbSNP:752608972
819 819 a, c, g dbSNP:776571416
820 820 a, g dbSNP:202217604
827 827 a, c dbSNP:751247865
831 831 a, g dbSNP:766068012
834 834 c, t dbSNP:28933973
835 835 a, g dbSNP:140281371
838 838 c, t dbSNP:764808366
843 843 a, g dbSNP:762455764
850 850 a, g dbSNP:753604724
854 854 c, t dbSNP:777261222
855 855 c, t dbSNP:550073023
856 856 a, g dbSNP:747380397
862 862 c, t dbSNP:775670991
863 863 a, g, t dbSNP:138508223
865 865 c, t dbSNP:142403457
870 870 a, g dbSNP:757315927
872 872 c, t dbSNP:748151937
878 878 g, t dbSNP:781114464
879 879 c, t dbSNP:754850577
880 880 a, g dbSNP:751390468
884 884 c, t dbSNP:766047301
887 887 c, t dbSNP:35329429
888 888 a, g dbSNP:766102404
896 896 c, g dbSNP:764894048
897 897 a, c dbSNP:552830871
899 899 a, g dbSNP:776211669
903 903 a, g dbSNP:764722375
904 904 g, t dbSNP:761049393
910 910 c, t dbSNP:775966864
911 911 a, g dbSNP:772306087
916 916 a, g dbSNP:28933375
917 917 c, t dbSNP:150053969
918 918 a, g dbSNP:771076819
926 926 g, t dbSNP:749385286
928 928 a, g dbSNP:777779022
936 936 a, g dbSNP:200368788
939 939 c, g dbSNP:746889280
941 941 c, t dbSNP:779948057
942 942 a, g dbSNP:758110629
951 951 a, g dbSNP:140218388
952 952 c, t dbSNP:764984064
957 957 a, g dbSNP:200824018
958 958 a, c, t dbSNP:763667216
967 967 a, g dbSNP:761293997
968 968 c, t dbSNP:147453010
969 969 a, g dbSNP:143043887
982 982 a, c, t dbSNP:747145070
983 983 c, t dbSNP:885821
985 985 a, c dbSNP:763119215
992 992 c, g dbSNP:773317614
997 997 a, g dbSNP:104894182
1011 1011 a, g dbSNP:199962500
1012 1012 a, g dbSNP:200716028
1013 1013 g, t dbSNP:747025790
1014 1014 -, aag dbSNP:745902829
1014 1014 a, c dbSNP:753226704
1027 1027 c, t dbSNP:150628656
1028 1028 a, g dbSNP:745670464
1031 1031 c, g, t dbSNP:376506267
1034 1034 c, t dbSNP:149008090
1046 1046 c, t dbSNP:757011029
1047 1047 c, t dbSNP:148237800
1049 1049 c, t dbSNP:777483971
1050 1050 c, t dbSNP:755578676
1051 1051 a, g dbSNP:752189303
1052 1052 a, g dbSNP:576966880
1055 1055 a, g dbSNP:760042830
1060 1060 a, g dbSNP:751950203
1061 1061 c, t dbSNP:885822
1064 1064 a, g dbSNP:763207236
1069 1069 a, t dbSNP:773405626
1076 1076 c, t dbSNP:769982593
1077 1077 a, g dbSNP:763002067
1082 1082 -, t dbSNP:777345151
1084 1084 a, t dbSNP:776713460
1085 1085 c, t dbSNP:771923778
1086 1086 a, g dbSNP:745770923
1087 1087 c, t dbSNP:754196056
1088 1088 c, t dbSNP:142643493
1091 1091 c, t dbSNP:770837333
1097 1097 c, t dbSNP:749015711
1098 1098 a, g dbSNP:777299577
1099 1099 a, t dbSNP:755737064
1100 1100 c, t dbSNP:752174520
1106 1106 a, g dbSNP:138159445
1109 1109 a, c, g, t dbSNP:201235771
1110 1110 a, g dbSNP:766783280
1112 1112 a, g dbSNP:201565901
1115 1115 c, t dbSNP:750718842
1118 1118 a, g dbSNP:554116546
1121 1121 c, t dbSNP:762044141
1122 1122 a, g dbSNP:145234214
1127 1127 c, t dbSNP:535958677
1128 1128 a, g dbSNP:375522165
1130 1130 a, g dbSNP:774426204
1133 1133 g, t dbSNP:770929453
1141 1141 c, t dbSNP:371021342
1146 1146 a, g dbSNP:749112632
1151 1151 c, t dbSNP:368769599
1153 1153 c, t dbSNP:769364825
1154 1154 a, g dbSNP:747803356
1160 1160 c, t dbSNP:140260618
1161 1161 a, c, g dbSNP:145463632
1162 1162 a, g dbSNP:780544163
1163 1163 c, g dbSNP:564568406
1166 1166 a, c dbSNP:758844674
1172 1172 a, c dbSNP:750774864
1175 1175 a, g dbSNP:765594736
1178 1178 a, g dbSNP:757472177
1179 1179 a, g dbSNP:754079962
1194 1194 a, c, t dbSNP:200641752
1195 1195 a, c, t dbSNP:28933374
1197 1197 c, t dbSNP:374261838
1199 1199 c, g dbSNP:762946719
1200 1200 c, t dbSNP:556996220
1202 1202 c, t dbSNP:150104693
1203 1203 a, c, g dbSNP:776338709
1208 1208 a, g, t dbSNP:771218500
1225 1225 c, t dbSNP:779488735
1226 1226 a, g dbSNP:758932748
1227 1227 c, t dbSNP:746365230
1228 1228 a, g dbSNP:571015630
1230 1230 c, t dbSNP:757636984
1231 1231 a, g dbSNP:140787739
1239 1239 c, t dbSNP:151083232
1241 1241 a, g dbSNP:756358874
1242 1242 a, t dbSNP:752858869
1251 1251 -, ct dbSNP:771552960
1259 1259 a, g dbSNP:767676771
1267 1267 c, t dbSNP:201584464
1268 1268 a, g dbSNP:145589960
1272 1272 a, g dbSNP:566891566
1273 1273 a, g dbSNP:199522390
1278 1278 c, g, t dbSNP:374588624
1279 1279 a, g dbSNP:140179771
1280 1280 a, c dbSNP:370384696
1283 1283 a, g dbSNP:104894176
1295 1295 a, c dbSNP:771559242
1297 1297 a, g dbSNP:746431351
1300 1300 c, g, t dbSNP:530097547
1301 1301 a, g dbSNP:144926997
1305 1305 c, t dbSNP:377367451
1306 1306 c, t dbSNP:756446764
1314 1314 c, t dbSNP:551046401
1315 1315 a, g dbSNP:781476866
1316 1316 a, g, t dbSNP:751523638
1317 1317 c, t dbSNP:765232909
1324 1324 g, t dbSNP:193302875
1325 1325 c, t dbSNP:761607073
1326 1326 c, t dbSNP:376289273
1328 1328 a, c dbSNP:763844396
1329 1329 c, t dbSNP:760379846
1330 1330 a, g dbSNP:775121659
1333 1333 a, t dbSNP:771647131
1335 1335 a, c dbSNP:759100720
1349 1349 a, g dbSNP:773688959
1355 1355 g, t dbSNP:148706255
1357 1357 g, t dbSNP:749729753
1360 1360 c, t dbSNP:778335116
1363 1363 c, t dbSNP:144242324
1364 1364 a, g dbSNP:748445504
1367 1367 c, t dbSNP:149581266
1368 1368 a, g dbSNP:371232368
1369 1369 c, t dbSNP:755093665
1372 1372 a, c, t dbSNP:779956870
1373 1373 c, t dbSNP:757256300
1380 1380 g, t dbSNP:753751595
1389 1389 c, t dbSNP:139322149
1390 1390 a, g dbSNP:150558419
1401 1401 c, g dbSNP:752431340
1405 1405 a, c, g dbSNP:759074628
1407 1407 c, t dbSNP:193302876
1410 1410 c, t dbSNP:765787505
1415 1415 a, c, g dbSNP:200789852
1420 1420 a, c dbSNP:770355235
1422 1422 c, t dbSNP:202216158
1423 1423 g, t dbSNP:200534908
1428 1428 c, t dbSNP:768953378
1435 1435 a, g dbSNP:142606828
1437 1437 a, g dbSNP:1042652
1444 1444 -, tgg dbSNP:747392486
1444 1444 g, t dbSNP:747646127
1447 1447 a, g dbSNP:104894181
1448 1448 a, g dbSNP:780229927
1457 1457 c, g dbSNP:752261431
1465 1465 c, t dbSNP:28933376
1466 1466 a, g dbSNP:200167080
1471 1471 c, t dbSNP:138126912
1473 1473 c, t dbSNP:755998254
1474 1474 a, g dbSNP:752523287
1478 1478 a, g dbSNP:767189454
1491 1491 -, ggtggc dbSNP:778216917
1495 1495 a, g dbSNP:754711256
1498 1498 a, c dbSNP:751161742
1500 1500 c, g dbSNP:145513232
1510 1510 c, t dbSNP:189650890
1511 1511 a, g dbSNP:200312496
1514 1514 c, t dbSNP:772785941
1517 1517 c, t dbSNP:765858178
1518 1518 a, g dbSNP:141864315
1526 1526 a, c dbSNP:777094015
1527 1527 a, c dbSNP:768878385
1534 1534 a, c dbSNP:528511999
1538 1538 a, c, t dbSNP:2228019
1539 1539 a, g dbSNP:772281903
1550 1550 a, g dbSNP:745970558
1551 1551 a, c, t dbSNP:754602851
1552 1552 a, g dbSNP:748060067
1567 1567 a, g dbSNP:577367079
1572 1572 c, t dbSNP:143040535
1581 1581 a, g dbSNP:751298400
1585 1585 a, g dbSNP:779584225
1588 1588 a, g, t dbSNP:377665096
1589 1589 -, g dbSNP:754622171
1589 1589 a, g dbSNP:145695221
1589 1589 -, g dbSNP:748926167
1591 1591 c, t dbSNP:762457806
1592 1592 c, t dbSNP:754285204
1593 1593 -, c dbSNP:779553489
1603 1603 a, c dbSNP:764596094
1605 1605 g, t dbSNP:760962796
1611 1611 a, g dbSNP:775875013
1612 1612 -, a dbSNP:755693770
1618 1618 a, c dbSNP:772313460
1631 1631 c, t dbSNP:759727585
1634 1634 c, t dbSNP:144968603
1639 1639 c, t dbSNP:774467483
1648 1648 c, t dbSNP:770987257
1660 1660 c, t dbSNP:375473178
1661 1661 a, t dbSNP:748186368
1662 1662 c, t dbSNP:200589152
1671 1671 c, g dbSNP:768577508
1679 1679 a, t dbSNP:746797570
1680 1680 g, t dbSNP:779855980
1682 1682 g, t dbSNP:200015777
1699 1699 -, aa dbSNP:749918235
1702 1702 a, g dbSNP:758163148
1719 1719 c, t dbSNP:377162454
1720 1720 a, g dbSNP:567204132
1721 1721 c, t dbSNP:147087107
1735 1735 a, g dbSNP:141702424
1744 1744 a, c dbSNP:753332095
1771 1771 a, g dbSNP:536292356
1777 1777 a, c dbSNP:764531237
1779 1779 a, c dbSNP:761206162
1781 1781 a, g dbSNP:149776121
1790 1790 g, t dbSNP:767848717
1797 1797 c, g dbSNP:550799905
1800 1800 a, c, g, t dbSNP:372713247
1804 1804 a, g dbSNP:144892262
1820 1820 c, t dbSNP:773289332
1821 1821 g, t dbSNP:768505698
1823 1823 a, g dbSNP:746931159
1838 1838 a, g dbSNP:779858353
1855 1855 c, t dbSNP:755876007
1860 1860 a, t dbSNP:376512084
1866 1866 a, t dbSNP:532610868
1867 1867 a, g dbSNP:571369275
1873 1873 a, g dbSNP:745582641
1875 1875 c, t dbSNP:140956842
1918 1918 c, t dbSNP:6480459
1924 1924 c, t dbSNP:560894722
1925 1925 a, g dbSNP:1889490
1933 1933 c, t dbSNP:752753581
1946 1946 c, t dbSNP:146571282
1952 1952 a, g dbSNP:563246193
1966 1966 g, t dbSNP:184146097
1968 1968 a, c, t dbSNP:56743633
2003 2003 c, t dbSNP:74513535
2005 2005 a, g dbSNP:573468737
2023 2023 -, ttt dbSNP:367570230
2024 2024 a, t dbSNP:79084177
2037 2037 -, t dbSNP:527442892
2038 2038 -, t dbSNP:34914326
2102 2102 a, g dbSNP:762398949
2195 2195 c, t dbSNP:139298157
2196 2196 c, g dbSNP:146245380
2205 2205 a, g dbSNP:191816002
2222 2222 c, t dbSNP:557290848
2237 2237 a, g dbSNP:143433002
2270 2270 a, g dbSNP:375066000
2295 2295 a, g dbSNP:546684854
2309 2309 a, g dbSNP:766663557
2310 2310 a, g dbSNP:187368446
2311 2311 c, t dbSNP:183113813
2334 2334 a, g dbSNP:368777270
2347 2347 g, t dbSNP:377317822
2350 2350 a, c dbSNP:193221010
2388 2388 g, t dbSNP:530803679
2434 2434 g, t dbSNP:563151587
2514 2514 a, g dbSNP:551388704

Target ORF information:

RefSeq Version NM_001083116
Organism Homo sapiens (human)
Definition Homo sapiens perforin 1 (pore forming protein) (PRF1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001083116

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCAGCCC GTCTGCTCCT CCTGGGCATC CTTCTCCTGC TGCTGCCCCT GCCCGTCCCT 
GCCCCGTGCC ACACAGCCGC ACGCTCAGAG TGCAAGCGCA GCCACAAGTT CGTGCCTGGT
GCATGGCTGG CCGGGGAGGG TGTGGACGTG ACCAGCCTCC GCCGCTCGGG CTCCTTCCCA
GTGGACACAC AAAGGTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGTGA AAATGCCCTA
CAGGAGGGCA CCCTCCAGCG CCTGCCTCTG GCGCTCACCA ACTGGCGGGC CCAGGGCTCT
GGCTGCCAGC GCCATGTAAC CAGGGCCAAA GTCAGCTCCA CTGAAGCTGT GGCCCGGGAT
GCGGCTCGTA GCATCCGCAA CGACTGGAAG GTCGGGCTGG ACGTGACTCC TAAGCCCACC
AGCAATGTGC ATGTGTCTGT GGCCGGCTCA CACTCACAGG CAGCCAACTT TGCAGCCCAG
AAGACCCACC AGGACCAGTA CAGCTTCAGC ACTGACACGG TGGAGTGCCG CTTCTACAGT
TTCCATGTGG TACACACTCC CCCGCTGCAC CCTGACTTCA AGAGGGCCCT CGGGGACCTG
CCCCACCACT TCAACGCCTC CACCCAGCCC GCCTACCTCA GGCTTATCTC CAACTACGGC
ACCCACTTCA TCCGGGCTGT GGAGCTGGGT GGCCGCATAT CGGCCCTCAC TGCCCTGCGC
ACCTGCGAGC TGGCCCTGGA AGGGCTCACG GACAACGAGG TGGAGGACTG CCTGACTGTC
GAGGCCCAGG TCAACATAGG CATCCACGGC AGCATCTCTG CCGAAGCCAA GGCCTGTGAG
GAGAAGAAGA AGAAGCACAA GATGACGGCC TCCTTCCACC AAACCTACCG GGAGCGCCAC
TCGGAAGTGG TTGGCGGCCA TCACACCTCC ATTAACGACC TGCTGTTCGG GATCCAGGCC
GGGCCCGAGC AGTACTCAGC CTGGGTAAAC TCGCTGCCCG GCAGCCCTGG CCTGGTGGAC
TACACCCTGG AACCCCTGCA CGTGCTGCTG GACAGCCAGG ACCCGCGGCG GGAGGCACTG
AGGAGGGCCC TGAGTCAGTA CCTGACGGAC AGGGCTCGCT GGAGGGACTG CAGCCGGCCG
TGCCCACCAG GGCGGCAGAA GAGCCCCCGA GACCCATGCC AGTGTGTGTG CCATGGCTCA
GCGGTCACCA CCCAGGACTG CTGCCCTCGG CAGAGGGGCC TGGCCCAGCT GGAGGTGACC
TTCATCCAAG CATGGGGCCT GTGGGGGGAC TGGTTCACTG CCACGGATGC CTATGTGAAG
CTCTTCTTTG GTGGCCAGGA GCTGAGGACG AGCACCGTGT GGGACAATAA CAACCCCATC
TGGTCAGTGC GGCTGGATTT TGGGGATGTG CTCCTGGCCA CAGGGGGGCC CCTGAGGTTG
CAGGTCTGGG ATCAGGACTC TGGCAGGGAC GATGACCTCC TTGGCACCTG TGATCAGGCT
CCCAAGTCTG GTTCCCATGA GGTGAGATGC AACCTGAATC ATGGCCACCT AAAATTCCGC
TATCATGCCA GGTGCTTGCC CCACCTGGGA GGAGGCACCT GCCTGGACTA TGTCCCCCAA
ATGCTTCTGG GGGAGCCTCC AGGAAACCGG AGTGGGGCCG TGTGGTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.