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PRKACA cDNA ORF clone, Homo sapiens (human)

Gene Symbol PRKACA
Entrez Gene ID 5566
Full Name protein kinase, cAMP-dependent, catalytic, alpha
Synonyms PKACA, PPNAD4
General protein information
Preferred Names
cAMP-dependent protein kinase catalytic subunit alpha
Names
cAMP-dependent protein kinase catalytic subunit alpha
PKA C-alpha
protein kinase A catalytic subunit
cAMP-dependent protein kinase catalytic subunit alpha, isoform 1
NP_002721.1
NP_997401.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19p13.1

Summary This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015]. lac of sum
Disorder MIM:

601639


mRNA and Protein(s)

mRNA Protein Name
NM_207518 NP_997401 cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha2
NM_001304349 NP_001291278 cAMP-dependent protein kinase catalytic subunit alpha isoform 3
NM_002730 NP_002721 cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1

KEGG
hsa04010 MAPK signaling pathway
hsa04020 Calcium signaling pathway
hsa04210 Apoptosis
hsa04310 Wnt signaling pathway
hsa04340 Hedgehog signaling pathway
hsa04540 Gap junction
hsa04720 Long-term potentiation
hsa04740 Olfactory transduction
hsa04742 Taste transduction
hsa04910 Insulin signaling pathway
hsa04912 GnRH signaling pathway
hsa04916 Melanogenesis
hsa05012 Parkinson's disease
hsa05110 Vibrio cholerae infection
hsa05200 Pathways in cancer
hsa04270 Vascular smooth muscle contraction
hsa04062 Chemokine signaling pathway
hsa05020 Prion diseases
hsa04914 Progesterone-mediated oocyte maturation
hsa05414 Dilated cardiomyopathy
hsa04114 Oocyte meiosis
hsa04962 Vasopressin-regulated water reabsorption
hsa04970 Salivary secretion
hsa04971 Gastric acid secretion
hsa05146 Amoebiasis
hsa04976 Bile secretion
hsa04961 Endocrine and other factor-regulated calcium reabsorption
hsa04724 Glutamatergic synapse
hsa04725 Cholinergic synapse
hsa05166 HTLV-I infection
hsa04727 GABAergic synapse
hsa04728 Dopaminergic synapse
hsa04726 Serotonergic synapse
hsa04723 Retrograde endocannabinoid signaling
hsa05030 Cocaine addiction
hsa05031 Amphetamine addiction
hsa05032 Morphine addiction
hsa05169 Epstein-Barr virus infection
hsa05034 Alcoholism
hsa05203 Viral carcinogenesis
hsa04713 Circadian entrainment
hsa04911 Insulin secretion
hsa05205 Proteoglycans in cancer
hsa04913 Ovarian steroidogenesis
hsa04915 Estrogen signaling pathway
hsa04918 Thyroid hormone synthesis
hsa04014 Ras signaling pathway
hsa04261 Adrenergic signaling in cardiomyocytes
hsa04919 Thyroid hormone signaling pathway
hsa04750 Inflammatory mediator regulation of TRP channels
hsa04611 Platelet activation
hsa04921 Oxytocin signaling pathway
hsa04024 cAMP signaling pathway
hsa_M00695 cAMP signaling
hsa04922 Glucagon signaling pathway
hsa04923 Regulation of lipolysis in adipocytes
hsa04924 Renin secretion
Reactome
R-HSA-112316 Neuronal System
R-HSA-112315 Transmission across Chemical Synapses
R-HSA-112314 Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell
R-HSA-442755 Activation of NMDA receptor upon glutamate binding and postsynaptic events
R-HSA-438064 Post NMDA receptor activation events
R-HSA-442720 CREB phosphorylation through the activation of Adenylate Cyclase
R-HSA-1852241 Organelle biogenesis and maintenance
R-HSA-5617833 Assembly of the primary cilium
R-HSA-5620912 Anchoring of the basal body to the plasma membrane
R-HSA-168256 Immune System
R-HSA-1280218 Adaptive Immune System
R-HSA-392517 Rap1 signalling
R-HSA-168249 Innate Immune System
R-HSA-2172127 DAP12 interactions
R-HSA-2424491 DAP12 signaling
R-HSA-1489509 DAG and IP3 signaling
R-HSA-111997 CaM pathway
R-HSA-111933 Calmodulin induced events
R-HSA-111931 PKA-mediated phosphorylation of CREB
R-HSA-163615 PKA activation
R-HSA-5621481 C-type lectin receptors (CLRs)
R-HSA-5621575 CD209 (DC-SIGN) signaling
R-HSA-1280215 Cytokine Signaling in Immune system
R-HSA-449147 Signaling by Interleukins
R-HSA-512988 Interleukin-3, 5 and GM-CSF signaling
R-HSA-109582 Hemostasis
R-HSA-983231 Factors involved in megakaryocyte development and platelet production
R-HSA-162582 Signal Transduction
R-HSA-177929 Signaling by EGFR
R-HSA-212718 EGFR interacts with phospholipase C-gamma
R-HSA-190236 Signaling by FGFR
R-HSA-5654736 Signaling by FGFR1
R-HSA-5654687 Downstream signaling of activated FGFR1
R-HSA-5654219 Phospholipase C-mediated cascade: FGFR1
R-HSA-5654738 Signaling by FGFR2
R-HSA-5654696 Downstream signaling of activated FGFR2
R-HSA-5654221 Phospholipase C-mediated cascade; FGFR2
R-HSA-5654741 Signaling by FGFR3
R-HSA-5654708 Downstream signaling of activated FGFR3
R-HSA-5654227 Phospholipase C-mediated cascade; FGFR3
R-HSA-5654743 Signaling by FGFR4
R-HSA-5654716 Downstream signaling of activated FGFR4
R-HSA-5654228 Phospholipase C-mediated cascade; FGFR4
R-HSA-166520 Signalling by NGF
R-HSA-187037 NGF signalling via TRKA from the plasma membrane
R-HSA-167021 PLC-gamma1 signalling
R-HSA-186797 Signaling by PDGF
R-HSA-186763 Downstream signal transduction
R-HSA-194138 Signaling by VEGF
R-HSA-4420097 VEGFA-VEGFR2 Pathway
R-HSA-1227986 Signaling by ERBB2
R-HSA-1251932 PLCG1 events in ERBB2 signaling
R-HSA-5683057 MAPK family signaling cascades
R-HSA-5687128 MAPK6/MAPK4 signaling
R-HSA-372790 Signaling by GPCR
R-HSA-111885 Opioid Signalling
R-HSA-112040 G-protein mediated events
R-HSA-112043 PLC beta mediated events
R-HSA-111996 Ca-dependent events
R-HSA-180024 DARPP-32 events
R-HSA-5358351 Signaling by Hedgehog
R-HSA-5610787 Hedgehog 'off' state
R-HSA-5610785 GLI3 is processed to GLI3R by the proteasome
R-HSA-5610783 Degradation of GLI2 by the proteasome
R-HSA-5610780 Degradation of GLI1 by the proteasome
R-HSA-1640170 Cell Cycle
R-HSA-69278 Cell Cycle, Mitotic
R-HSA-453274 Mitotic G2-G2/M phases
R-HSA-69275 G2/M Transition
R-HSA-2565942 Regulation of PLK1 Activity at G2/M Transition
R-HSA-380287 Centrosome maturation
R-HSA-380270 Recruitment of mitotic centrosome proteins and complexes
R-HSA-380284 Loss of proteins required for interphase microtubule organizationfrom the centrosome
R-HSA-380259 Loss of Nlp from mitotic centrosomes
R-HSA-382551 Transmembrane transport of small molecules
R-HSA-445717 Aquaporin-mediated transport
R-HSA-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-HSA-1430728 Metabolism
R-HSA-71387 Metabolism of carbohydrates
R-HSA-70326 Glucose metabolism
R-HSA-70263 Gluconeogenesis
R-HSA-556833 Metabolism of lipids and lipoproteins
R-HSA-73923 Lipid digestion, mobilization, and transport
R-HSA-163560 Hormone-sensitive lipase (HSL)-mediated triacylglycerol hydrolysis
R-HSA-163685 Integration of energy metabolism
R-HSA-422356 Regulation of insulin secretion
R-HSA-381676 Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
R-HSA-163359 Glucagon signaling in metabolic regulation
R-HSA-164378 PKA activation in glucagon signalling
R-HSA-163358 PKA-mediated phosphorylation of key metabolic factors
Pathway Interaction Database
hedgehog_glipathway Hedgehog signaling events mediated by Gli proteins
ret_pathway Signaling events regulated by Ret tyrosine kinase
vegfr1_pathway VEGFR1 specific signals
aurora_a_pathway Aurora A signaling
vegfr1_2_pathway Signaling events mediated by VEGFR1 and VEGFR2
tcrcalciumpathway Calcium signaling in the CD4+ TCR pathway
lpa4_pathway LPA4-mediated signaling events
nfat_3pathway Role of Calcineurin-dependent NFAT signaling in lymphocytes
erbb2erbb3pathway ErbB2/ErbB3 signaling events
lkb1_pathway LKB1 signaling events
hdac_classi_pathway Signaling events mediated by HDAC Class I
reg_gr_pathway Glucocorticoid receptor regulatory network
pi3kciaktpathway Class I PI3K signaling events mediated by Akt
a4b1_paxdep_pathway Paxillin-dependent events mediated by a4b1
syndecan_2_pathway Syndecan-2-mediated signaling events
retinoic_acid_pathway Retinoic acid receptors-mediated signaling
syndecan_1_pathway Syndecan-1-mediated signaling events
txa2pathway Thromboxane A2 receptor signaling
WikiPathways
WP289 Myometrial Relaxation and Contraction Pathways
WP666 Hypothetical Network for Drug Addiction
WP382 MAPK signaling pathway
WP474 Endochondral Ossification
WP35 G Protein Signaling Pathways
WP1602 Nicotine Activity on Dopaminergic Neurons
WP712 Estrogen signaling pathway
WP286 IL-3 Signaling Pathway
WP231 TNF-alpha/NF-kB Signaling Pathway
WP706 SIDS Susceptibility Pathways
WP536 Calcium Regulation in the Cardiac Cell
WP1982 SREBP signalling
WP2012 miRs in Muscle Cell Differentiation
WP2035 FSH signaling pathway
WP2038 Regulation of Microtubule Cytoskeleton
WP2436 Dopamine metabolism


Homo sapiens (human) PRKACA NP_002721.1
Pan troglodytes (chimpanzee) PRKACA XP_001171437.1
Canis lupus familiaris (dog) PRKACA NP_001003032.1
Bos taurus (cattle) PRKACA NP_777009.1
Mus musculus (house mouse) Prkaca NP_032880.1
Rattus norvegicus (Norway rat) Prkaca NP_001094392.1
Danio rerio (zebrafish) prkacab NP_001003470.1
Xenopus (Silurana) tropicalis (western clawed frog) prkaca NP_001016403.1

Component

ID Name Evidence
GO:0005624 membrane fraction IDA
GO:0005625 soluble fraction IEA
GO:0005634 nucleus IEA
GO:0005654 nucleoplasm TAS
GO:0005737 cytoplasm IEA
GO:0005739 mitochondrion IEA
GO:0005794 Golgi apparatus IEA
GO:0005829 cytosol EXP
GO:0005829 cytosol TAS
GO:0005886 plasma membrane IEA
GO:0005952 cAMP-dependent protein kinase complex NAS
GO:0031594 neuromuscular junction IEA
GO:0048471 perinuclear region of cytoplasm IEA

Function

ID Name Evidence
GO:0000166 nucleotide binding IEA
GO:0004674 protein serine/threonine kinase activity EXP
GO:0004674 protein serine/threonine kinase activity TAS
GO:0004691 cAMP-dependent protein kinase activity NAS
GO:0004691 cAMP-dependent protein kinase activity TAS
GO:0004862 cAMP-dependent protein kinase inhibitor activity EXP
GO:0005515 protein binding IPI
GO:0005524 ATP binding NAS
GO:0019901 protein kinase binding IPI
GO:0032403 protein complex binding IEA

Process

ID Name Evidence
GO:0000086 G2/M transition of mitotic cell cycle TAS
GO:0000278 mitotic cell cycle TAS
GO:0001707 mesoderm formation IEA
GO:0005975 carbohydrate metabolic process TAS
GO:0006006 glucose metabolic process TAS
GO:0006094 gluconeogenesis TAS
GO:0006112 energy reserve metabolic process TAS
GO:0006468 protein phosphorylation NAS
GO:0006629 lipid metabolic process TAS
GO:0006833 water transport TAS
GO:0007202 activation of phospholipase C activity TAS
GO:0007243 intracellular protein kinase cascade EXP
GO:0007596 blood coagulation TAS
GO:0018105 peptidyl-serine phosphorylation IEA
GO:0019433 triglyceride catabolic process TAS
GO:0034199 activation of protein kinase A activity EXP
GO:0043457 regulation of cellular respiration IEA
GO:0046777 protein autophosphorylation IEA
GO:0046827 positive regulation of protein export from nucleus IEA
GO:0048011 nerve growth factor receptor signaling pathway TAS
GO:0050796 regulation of insulin secretion TAS
GO:0051447 negative regulation of meiotic cell cycle IEA
GO:0051966 regulation of synaptic transmission, glutamatergic IEA
GO:0055085 transmembrane transport TAS
GO:0071374 cellular response to parathyroid hormone stimulus IEA
GO:0071377 cellular response to glucagon stimulus EXP

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PRKACA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKACA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu17314 NM_207518 Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $202.30
$289.00
OHu51697 NM_001304349 Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu18669
NM_002730 Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $189.50
$379.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu17314
Clone ID Related Accession (Same CDS sequence) NM_207518
Accession Version NM_207518.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1032bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292284.1, BC108259.1 and BC039846.1. On Jan 17, 2015 this sequence version replaced gi:46909583. Summary: This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015]. Transcript Variant: This variant (2) contains an alternate 5' terminal exon and initiates translation at an alternate start codon, compared to variant 1. It encodes Calpha2, PMID:21812984 (also known as C alpha-s, PMID: 10906071) which has a shorter and distinct N-terminus, compared to Calpha1. This variant is specifically expressed in spermatogenic cells and may be essential for male fertility. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292284.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962337, SAMEA962343 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTTCCA ACTCCAGCGA TGTGAAAGAA TTCTTAGCCA AAGCCAAAGA AGATTTTCTT 
AAAAAATGGG AAAGTCCCGC TCAGAACACA GCCCACTTGG ATCAGTTTGA ACGAATCAAG
ACCCTCGGCA CGGGCTCCTT CGGGCGGGTG ATGCTGGTGA AACACAAGGA GACCGGGAAC
CACTATGCCA TGAAGATCCT CGACAAACAG AAGGTGGTGA AACTGAAACA GATCGAACAC
ACCCTGAATG AAAAGCGCAT CCTGCAAGCT GTCAACTTTC CGTTCCTCGT CAAACTCGAG
TTCTCCTTCA AGGACAACTC AAACTTATAC ATGGTCATGG AGTACGTGCC CGGCGGGGAG
ATGTTCTCAC ACCTACGGCG GATCGGAAGG TTCAGTGAGC CCCATGCCCG TTTCTACGCG
GCCCAGATCG TCCTGACCTT TGAGTATCTG CACTCGCTGG ATCTCATCTA CAGGGACCTG
AAGCCGGAGA ATCTGCTCAT TGACCAGCAG GGCTACATTC AGGTGACAGA CTTCGGTTTC
GCCAAGCGCG TGAAGGGCCG CACTTGGACC TTGTGCGGCA CCCCTGAGTA CCTGGCCCCT
GAGATTATCC TGAGCAAAGG CTACAACAAG GCCGTGGACT GGTGGGCCCT GGGGGTTCTT
ATCTATGAAA TGGCCGCTGG CTACCCGCCC TTCTTCGCAG ACCAGCCCAT CCAGATCTAT
GAGAAGATCG TCTCTGGGAA GGTGCGCTTC CCTTCCCACT TCAGCTCTGA CTTGAAGGAC
CTGCTGCGGA ACCTCCTGCA GGTAGATCTC ACCAAGCGCT TTGGGAACCT CAAGAATGGG
GTCAACGATA TCAAGAACCA CAAGTGGTTT GCCACAACTG ACTGGATTGC CATCTACCAG
AGGAAGGTGG AAGCTCCCTT CATACCAAAG TTTAAAGGCC CTGGGGATAC GAGTAACTTT
GACGACTATG AGGAAGAAGA AATCCGGGTC TCCATCAATG AGAAGTGTGG CAAGGAGTTT
TCTGAGTTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_997401.1
CDS34..1065
Misc Feature(1)28..30(+)
Misc Feature(2)133..1002(+)
Misc Feature(3)139..903(+)
Misc Feature(4)157..993(+)
Misc Feature(5)160..1002(+)
Misc Feature(6)163..1002(+)
Misc Feature(7)169..1002(+)
Misc Feature(8)427..429(+)
Misc Feature(9)559..621(+)
Misc Feature(10)595..597(+)
Misc Feature(11)601..603(+)
Misc Feature(12)613..615(+)
Misc Feature(13)1024..1026(+)
Exon (1)1..55
Gene:PRKACA
Gene Synonym:
Exon (2)56..117
Gene:PRKACA
Gene Synonym:
Exon (3)118..246
Gene:PRKACA
Gene Synonym:
Exon (4)247..345
Gene:PRKACA
Gene Synonym:
Exon (5)346..428
Gene:PRKACA
Gene Synonym:
Exon (6)429..555
Gene:PRKACA
Gene Synonym:
Exon (7)556..651
Gene:PRKACA
Gene Synonym:
Exon (8)652..774
Gene:PRKACA
Gene Synonym:
Exon (9)775..939
Gene:PRKACA
Gene Synonym:
Exon (10)940..2489
Gene:PRKACA
Gene Synonym:
Translation
Position Chain Variation Link
1 1 a, g dbSNP:775988368
7 7 c, t dbSNP:200461298
8 8 a, c, t dbSNP:370452277
9 9 -, ggtgcc dbSNP:757453058
9 9 a, g dbSNP:375578437
17 17 a, g, t dbSNP:777958755
21 21 a, c, g dbSNP:369958809
22 22 c, t dbSNP:765166143
23 23 a, t dbSNP:754717890
24 24 a, g dbSNP:753663497
25 25 a, g dbSNP:200209462
28 28 c, t dbSNP:760423325
31 31 a, g dbSNP:376311025
32 32 c, t dbSNP:756630808
35 35 g, t dbSNP:201062704
38 38 c, t dbSNP:202232211
43 43 a, c dbSNP:761410000
46 46 c, t dbSNP:776080100
50 50 a, g dbSNP:770449257
51 51 a, c dbSNP:376122267
99 99 a, g dbSNP:771393106
104 104 a, c dbSNP:760954437
111 111 c, t dbSNP:56105247
112 112 a, g dbSNP:142045517
116 116 a, g dbSNP:748193038
120 120 c, t dbSNP:767936415
122 122 c, t dbSNP:762166548
125 125 c, g dbSNP:774495964
130 130 g, t dbSNP:56029020
145 145 a, c dbSNP:764242716
146 146 a, g dbSNP:56085217
158 158 a, c, t dbSNP:112360106
159 159 c, t dbSNP:775474906
165 165 a, c, g dbSNP:745853839
174 174 c, t dbSNP:776568028
177 177 a, g, t dbSNP:373468300
179 179 a, g dbSNP:746814323
194 194 a, g dbSNP:777520540
199 199 -, aag dbSNP:762588021
200 200 a, g dbSNP:758020326
203 203 a, g dbSNP:747669394
205 205 -, acc dbSNP:752551387
205 205 a, c, g dbSNP:201769960
207 207 c, g, t dbSNP:768020526
208 208 c, g dbSNP:757565596
217 217 c, t dbSNP:201225070
224 224 c, t dbSNP:764342858
234 234 c, t dbSNP:763136554
235 235 a, g dbSNP:369286962
241 241 c, t dbSNP:765305631
249 249 a, g dbSNP:773054959
267 267 c, t dbSNP:368239098
271 271 c, t dbSNP:376284068
275 275 c, g dbSNP:773821844
280 280 a, c dbSNP:768324869
289 289 c, t dbSNP:748763072
290 290 a, g dbSNP:779586629
300 300 a, g dbSNP:371232485
314 314 c, g dbSNP:747585702
315 315 a, g dbSNP:202032822
319 319 c, t dbSNP:758631881
321 321 c, t dbSNP:753127913
327 327 a, g dbSNP:376182047
330 330 c, g, t dbSNP:148811989
331 331 c, g dbSNP:766631376
350 350 a, g dbSNP:369058901
362 362 a, g dbSNP:775066482
363 363 c, t dbSNP:376915447
364 364 a, g dbSNP:758933458
378 378 c, t dbSNP:201100703
384 384 c, t dbSNP:772438487
390 390 a, g dbSNP:78098302
409 409 c, t dbSNP:775000520
412 412 c, t dbSNP:769081071
413 413 a, g dbSNP:749688339
417 417 c, t dbSNP:141320866
418 418 a, g dbSNP:148280386
422 422 c, g dbSNP:756398151
435 435 c, t dbSNP:777648038
438 438 a, t dbSNP:758375271
450 450 c, t dbSNP:558688172
452 452 a, c dbSNP:778829250
453 453 c, g dbSNP:754730934
459 459 a, g dbSNP:753469180
462 462 c, t dbSNP:145751355
470 470 a, c dbSNP:765966838
471 471 c, g dbSNP:760188119
475 475 a, g dbSNP:749992796
489 489 a, g dbSNP:764675218
519 519 a, g dbSNP:763611702
525 525 c, t dbSNP:775990456
526 526 a, c dbSNP:537279998
528 528 g, t dbSNP:759833330
533 533 c, t dbSNP:776865039
540 540 g, t dbSNP:771089857
541 541 a, c dbSNP:201595411
549 549 c, t dbSNP:745485648
550 550 a, g dbSNP:142007512
552 552 a, t dbSNP:748153891
558 558 a, c, g dbSNP:746473377
567 567 c, t dbSNP:761035852
569 569 g, t dbSNP:11541563
573 573 c, t dbSNP:773370977
574 574 a, g dbSNP:767755931
580 580 c, t dbSNP:761845520
581 581 a, g dbSNP:774340605
582 582 c, t dbSNP:768624241
583 583 a, g dbSNP:372210586
593 593 a, g dbSNP:777156218
604 604 c, t dbSNP:775500643
608 608 -, gtg dbSNP:724160013
609 609 c, t dbSNP:769653802
618 618 c, t dbSNP:745681054
626 626 g, t dbSNP:386352352
636 636 a, g dbSNP:780953754
642 642 a, c dbSNP:770747549
646 646 a, c dbSNP:746506291
654 654 c, t dbSNP:756658679
658 658 a, c dbSNP:750769824
666 666 c, t dbSNP:781602713
681 681 c, g dbSNP:757644974
682 682 -, c dbSNP:34988264
687 687 a, g dbSNP:751673161
711 711 c, t dbSNP:764397826
720 720 a, g dbSNP:137911238
726 726 c, t dbSNP:752866900
731 731 c, t dbSNP:765243751
738 738 a, g dbSNP:759605652
741 741 c, t dbSNP:776566422
762 762 c, t dbSNP:770568845
763 763 a, g dbSNP:760535486
778 778 c, t dbSNP:773941420
779 779 a, g dbSNP:763766541
793 793 a, t dbSNP:762420208
799 799 c, t dbSNP:775116225
800 800 c, g dbSNP:35635531
836 836 g, t dbSNP:761312324
852 852 c, g dbSNP:3729858
857 857 a, g dbSNP:772509949
861 861 c, t dbSNP:748549700
873 873 a, g dbSNP:774370135
878 878 a, g dbSNP:779240866
882 882 c, t dbSNP:769046142
884 884 c, t dbSNP:749362012
915 915 c, g, t dbSNP:756149376
922 922 a, g dbSNP:750316086
927 927 c, t dbSNP:781011594
930 930 c, t dbSNP:200573717
933 933 a, g dbSNP:149272491
935 935 a, g dbSNP:187770246
936 936 a, g dbSNP:371711255
951 951 c, t dbSNP:145797642
961 961 a, c dbSNP:752340563
964 964 c, t dbSNP:149832080
970 970 c, g dbSNP:373941966
979 979 a, g dbSNP:759051524
981 981 c, t dbSNP:753333407
984 984 a, g dbSNP:370893378
987 987 c, t dbSNP:553187237
996 996 c, t dbSNP:762472510
1002 1002 c, t dbSNP:775000550
1005 1005 a, g dbSNP:370912699
1018 1018 c, t dbSNP:573921316
1019 1019 a, g dbSNP:377316394
1031 1031 a, g dbSNP:775739774
1035 1035 c, g dbSNP:769948900
1042 1042 a, g dbSNP:745937862
1054 1054 c, t dbSNP:141087932
1057 1057 a, g dbSNP:770850404
1059 1059 c, g dbSNP:746884607
1068 1068 a, g dbSNP:777703093
1073 1073 a, c dbSNP:186700926
1074 1074 c, g dbSNP:747928705
1076 1076 c, g dbSNP:41296312
1077 1077 g, t dbSNP:754638938
1079 1079 a, c dbSNP:574054401
1083 1083 c, t dbSNP:765916882
1085 1085 a, t dbSNP:41296314
1086 1086 a, g dbSNP:368971640
1088 1088 a, g, t dbSNP:763345212
1090 1090 c, t dbSNP:775765231
1093 1093 c, t dbSNP:765484342
1100 1100 c, t dbSNP:759743296
1103 1103 -, tttt dbSNP:777513827
1107 1107 -, ctttttt dbSNP:141786059
1107 1107 -, c dbSNP:758345813
1115 1115 -, t dbSNP:754820234
1115 1115 -, t dbSNP:765052810
1124 1124 g, t dbSNP:540631278
1133 1133 a, g dbSNP:540043364
1153 1153 a, g dbSNP:41296316
1203 1203 a, g dbSNP:546874591
1203 1203 -, g dbSNP:35571116
1259 1259 c, t dbSNP:558194814
1307 1307 c, t dbSNP:11541565
1350 1350 c, t dbSNP:575871334
1408 1408 a, c dbSNP:41296320
1413 1413 c, t dbSNP:770941968
1447 1447 a, c dbSNP:11541564
1465 1465 c, t dbSNP:773096384
1514 1514 a, g dbSNP:41296322
1522 1522 g, t dbSNP:535704731
1564 1564 a, g dbSNP:192062046
1598 1598 a, t dbSNP:41296324
1599 1599 a, t dbSNP:533644410
1648 1648 c, t dbSNP:771557895
1650 1650 c, t dbSNP:374398241
1688 1688 c, t dbSNP:372738854
1740 1740 a, g dbSNP:371294220
1764 1764 a, t dbSNP:751142658
1780 1780 g, t dbSNP:761592242
1819 1819 a, g dbSNP:533264551
1842 1842 -, ctct dbSNP:750815980
1844 1844 -, ct dbSNP:536003350
1849 1849 c, g, t dbSNP:181393862
1851 1851 c, t dbSNP:551104459
1959 1959 a, g dbSNP:533224290
1974 1974 c, t dbSNP:773836816
1978 1978 a, g dbSNP:768206023
1987 1987 c, t dbSNP:189384496
1997 1997 c, t dbSNP:550733152
2011 2011 c, t dbSNP:529114892
2016 2016 c, t dbSNP:138923029
2027 2027 a, g dbSNP:780499415
2033 2033 a, c dbSNP:770356716
2036 2036 a, t dbSNP:540693985
2120 2120 c, t dbSNP:746164515
2146 2146 c, g dbSNP:373483298
2153 2153 -, tcac dbSNP:537314114
2178 2178 a, t dbSNP:781626359
2198 2198 a, g dbSNP:564446879
2227 2227 a, c dbSNP:184594472
2253 2253 -, t dbSNP:41296326
2263 2263 c, t dbSNP:757419024
2269 2269 a, c dbSNP:575879842
2296 2296 a, g dbSNP:41296328
2302 2302 a, c dbSNP:15247
2319 2319 c, t dbSNP:751891973
2351 2351 c, g dbSNP:369471735
2375 2375 a, g dbSNP:181006322
2433 2433 g, t dbSNP:189139001

Target ORF information:

RefSeq Version NM_207518
Organism Homo sapiens (human)
Definition Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_207518

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTTCCA ACTCCAGCGA TGTGAAAGAA TTCTTAGCCA AAGCCAAAGA AGATTTTCTT 
AAAAAATGGG AAAGTCCCGC TCAGAACACA GCCCACTTGG ATCAGTTTGA ACGAATCAAG
ACCCTCGGCA CGGGCTCCTT CGGGCGGGTG ATGCTGGTGA AACACAAGGA GACCGGGAAC
CACTATGCCA TGAAGATCCT CGACAAACAG AAGGTGGTGA AACTGAAACA GATCGAACAC
ACCCTGAATG AAAAGCGCAT CCTGCAAGCT GTCAACTTTC CGTTCCTCGT CAAACTCGAG
TTCTCCTTCA AGGACAACTC AAACTTATAC ATGGTCATGG AGTACGTGCC CGGCGGGGAG
ATGTTCTCAC ACCTACGGCG GATCGGAAGG TTCAGTGAGC CCCATGCCCG TTTCTACGCG
GCCCAGATCG TCCTGACCTT TGAGTATCTG CACTCGCTGG ATCTCATCTA CAGGGACCTG
AAGCCGGAGA ATCTGCTCAT TGACCAGCAG GGCTACATTC AGGTGACAGA CTTCGGTTTC
GCCAAGCGCG TGAAGGGCCG CACTTGGACC TTGTGCGGCA CCCCTGAGTA CCTGGCCCCT
GAGATTATCC TGAGCAAAGG CTACAACAAG GCCGTGGACT GGTGGGCCCT GGGGGTTCTT
ATCTATGAAA TGGCCGCTGG CTACCCGCCC TTCTTCGCAG ACCAGCCCAT CCAGATCTAT
GAGAAGATCG TCTCTGGGAA GGTGCGCTTC CCTTCCCACT TCAGCTCTGA CTTGAAGGAC
CTGCTGCGGA ACCTCCTGCA GGTAGATCTC ACCAAGCGCT TTGGGAACCT CAAGAATGGG
GTCAACGATA TCAAGAACCA CAAGTGGTTT GCCACAACTG ACTGGATTGC CATCTACCAG
AGGAAGGTGG AAGCTCCCTT CATACCAAAG TTTAAAGGCC CTGGGGATAC GAGTAACTTT
GACGACTATG AGGAAGAAGA AATCCGGGTC TCCATCAATG AGAAGTGTGG CAAGGAGTTT
TCTGAGTTTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu51697
Clone ID Related Accession (Same CDS sequence) NM_001304349
Accession Version NM_001304349.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cAMP-dependent protein kinase catalytic subunit alpha isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK131561.1, BC108259.1 and BC039846.1. On Jan 17, 2015 this sequence version replaced gi:530415007. Summary: This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015]. Transcript Variant: This variant (3) contains an alternate 5' terminal exon and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 3 which has a longer and distinct N-terminus, compared to Calpha1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK131561.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159607, SAMEA962337 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTTCACCT TGGGATACCA TGGGGCCGAG GAGTGGGCAT CGGGCGAGAC TTCCCTTGAA 
CCAGCTCTGG AGCAATGCCC TTGCGATTGC CACGGAGAGG AACAGGGCCA GCTGGGGGCC
AAGGTGACAG CTGAGCCCCC CAAGGGCACC TGCTACCCGA GCCCCCGAGG CCTCCATCCC
AGGCCCGGCC TCCCCAGAGA AGGGGCCAGA GGAACAGCCA AATCCCAAGG CTATAAATAC
CTCGGCTGGC GTCGGCCCTG GCCATGGAAT GTCGTGAAAG AATTCTTAGC CAAAGCCAAA
GAAGATTTTC TTAAAAAATG GGAAAGTCCC GCTCAGAACA CAGCCCACTT GGATCAGTTT
GAACGAATCA AGACCCTCGG CACGGGCTCC TTCGGGCGGG TGATGCTGGT GAAACACAAG
GAGACCGGGA ACCACTATGC CATGAAGATC CTCGACAAAC AGAAGGTGGT GAAACTGAAA
CAGATCGAAC ACACCCTGAA TGAAAAGCGC ATCCTGCAAG CTGTCAACTT TCCGTTCCTC
GTCAAACTCG AGTTCTCCTT CAAGGACAAC TCAAACTTAT ACATGGTCAT GGAGTACGTG
CCCGGCGGGG AGATGTTCTC ACACCTACGG CGGATCGGAA GGTTCAGTGA GCCCCATGCC
CGTTTCTACG CGGCCCAGAT CGTCCTGACC TTTGAGTATC TGCACTCGCT GGATCTCATC
TACAGGGACC TGAAGCCGGA GAATCTGCTC ATTGACCAGC AGGGCTACAT TCAGGTGACA
GACTTCGGTT TCGCCAAGCG CGTGAAGGGC CGCACTTGGA CCTTGTGCGG CACCCCTGAG
TACCTGGCCC CTGAGATTAT CCTGAGCAAA GGCTACAACA AGGCCGTGGA CTGGTGGGCC
CTGGGGGTTC TTATCTATGA AATGGCCGCT GGCTACCCGC CCTTCTTCGC AGACCAGCCC
ATCCAGATCT ATGAGAAGAT CGTCTCTGGG AAGGTGCGCT TCCCTTCCCA CTTCAGCTCT
GACTTGAAGG ACCTGCTGCG GAACCTCCTG CAGGTAGATC TCACCAAGCG CTTTGGGAAC
CTCAAGAATG GGGTCAACGA TATCAAGAAC CACAAGTGGT TTGCCACAAC TGACTGGATT
GCCATCTACC AGAGGAAGGT GGAAGCTCCC TTCATACCAA AGTTTAAAGG CCCTGGGGAT
ACGAGTAACT TTGACGACTA TGAGGAAGAA GAAATCCGGG TCTCCATCAA TGAGAAGTGT
GGCAAGGAGT TTTCTGAGTT TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001291278.1
CDS407..1690
Misc Feature(1)341..343(+)
Misc Feature(2)758..1627(+)
Misc Feature(3)764..1528(+)
Misc Feature(4)782..1618(+)
Misc Feature(5)785..1627(+)
Misc Feature(6)788..1627(+)
Misc Feature(7)794..1627(+)
Misc Feature(8)1184..1246(+)
Exon (1)1..680
Gene:PRKACA
Gene Synonym:
Exon (2)681..742
Gene:PRKACA
Gene Synonym:
Exon (3)743..871
Gene:PRKACA
Gene Synonym:
Exon (4)872..970
Gene:PRKACA
Gene Synonym:
Exon (5)971..1053
Gene:PRKACA
Gene Synonym:
Exon (6)1054..1180
Gene:PRKACA
Gene Synonym:
Exon (7)1181..1276
Gene:PRKACA
Gene Synonym:
Exon (8)1277..1399
Gene:PRKACA
Gene Synonym:
Exon (9)1400..1564
Gene:PRKACA
Gene Synonym:
Exon (10)1565..3114
Gene:PRKACA
Gene Synonym:
Translation
Position Chain Variation Link
88 88 a, t dbSNP:756550513
94 94 c, t dbSNP:371693057
98 98 a, g dbSNP:561068444
104 104 c, g dbSNP:186989764
132 132 g, t dbSNP:139061971
207 207 c, g dbSNP:184562247
210 210 c, t dbSNP:192050186
212 212 -, c dbSNP:34477737
290 290 c, g dbSNP:556657823
300 300 a, t dbSNP:545687014
308 308 a, g dbSNP:545426521
329 329 a, g dbSNP:757613568
354 354 a, g dbSNP:533564139
362 362 c, t dbSNP:748702690
378 378 a, g dbSNP:751776951
452 452 g, t dbSNP:367744279
464 464 c, g dbSNP:563023667
470 470 a, g dbSNP:188008604
499 499 c, t dbSNP:544838575
500 500 a, g dbSNP:41296250
547 547 c, t dbSNP:554735660
564 564 c, t dbSNP:41296252
572 572 c, t dbSNP:571993209
592 592 c, t dbSNP:35931031
593 593 c, g dbSNP:746536971
636 636 a, g dbSNP:538515678
647 647 c, t dbSNP:150735079
650 650 a, g dbSNP:41296254
657 657 g, t dbSNP:144311902
659 659 c, t dbSNP:752756840
660 660 a, g dbSNP:765475159
680 680 c, g dbSNP:567406683
724 724 a, g dbSNP:771393106
729 729 a, c dbSNP:760954437
736 736 c, t dbSNP:56105247
737 737 a, g dbSNP:142045517
741 741 a, g dbSNP:748193038
745 745 c, t dbSNP:767936415
747 747 c, t dbSNP:762166548
750 750 c, g dbSNP:774495964
755 755 g, t dbSNP:56029020
770 770 a, c dbSNP:764242716
771 771 a, g dbSNP:56085217
783 783 a, c, t dbSNP:112360106
784 784 c, t dbSNP:775474906
790 790 a, c, g dbSNP:745853839
799 799 c, t dbSNP:776568028
802 802 a, g, t dbSNP:373468300
804 804 a, g dbSNP:746814323
819 819 a, g dbSNP:777520540
824 824 -, aag dbSNP:762588021
825 825 a, g dbSNP:758020326
828 828 a, g dbSNP:747669394
830 830 -, acc dbSNP:752551387
830 830 a, c, g dbSNP:201769960
832 832 c, g, t dbSNP:768020526
833 833 c, g dbSNP:757565596
842 842 c, t dbSNP:201225070
849 849 c, t dbSNP:764342858
859 859 c, t dbSNP:763136554
860 860 a, g dbSNP:369286962
866 866 c, t dbSNP:765305631
874 874 a, g dbSNP:773054959
892 892 c, t dbSNP:368239098
896 896 c, t dbSNP:376284068
900 900 c, g dbSNP:773821844
905 905 a, c dbSNP:768324869
914 914 c, t dbSNP:748763072
915 915 a, g dbSNP:779586629
925 925 a, g dbSNP:371232485
939 939 c, g dbSNP:747585702
940 940 a, g dbSNP:202032822
944 944 c, t dbSNP:758631881
946 946 c, t dbSNP:753127913
952 952 a, g dbSNP:376182047
955 955 c, g, t dbSNP:148811989
956 956 c, g dbSNP:766631376
975 975 a, g dbSNP:369058901
987 987 a, g dbSNP:775066482
988 988 c, t dbSNP:376915447
989 989 a, g dbSNP:758933458
1003 1003 c, t dbSNP:201100703
1009 1009 c, t dbSNP:772438487
1015 1015 a, g dbSNP:78098302
1034 1034 c, t dbSNP:775000520
1037 1037 c, t dbSNP:769081071
1038 1038 a, g dbSNP:749688339
1042 1042 c, t dbSNP:141320866
1043 1043 a, g dbSNP:148280386
1047 1047 c, g dbSNP:756398151
1060 1060 c, t dbSNP:777648038
1063 1063 a, t dbSNP:758375271
1075 1075 c, t dbSNP:558688172
1077 1077 a, c dbSNP:778829250
1078 1078 c, g dbSNP:754730934
1084 1084 a, g dbSNP:753469180
1087 1087 c, t dbSNP:145751355
1095 1095 a, c dbSNP:765966838
1096 1096 c, g dbSNP:760188119
1100 1100 a, g dbSNP:749992796
1114 1114 a, g dbSNP:764675218
1144 1144 a, g dbSNP:763611702
1150 1150 c, t dbSNP:775990456
1151 1151 a, c dbSNP:537279998
1153 1153 g, t dbSNP:759833330
1158 1158 c, t dbSNP:776865039
1165 1165 g, t dbSNP:771089857
1166 1166 a, c dbSNP:201595411
1174 1174 c, t dbSNP:745485648
1175 1175 a, g dbSNP:142007512
1177 1177 a, t dbSNP:748153891
1183 1183 a, c, g dbSNP:746473377
1192 1192 c, t dbSNP:761035852
1194 1194 g, t dbSNP:11541563
1198 1198 c, t dbSNP:773370977
1199 1199 a, g dbSNP:767755931
1205 1205 c, t dbSNP:761845520
1206 1206 a, g dbSNP:774340605
1207 1207 c, t dbSNP:768624241
1208 1208 a, g dbSNP:372210586
1218 1218 a, g dbSNP:777156218
1229 1229 c, t dbSNP:775500643
1233 1233 -, gtg dbSNP:724160013
1234 1234 c, t dbSNP:769653802
1243 1243 c, t dbSNP:745681054
1251 1251 g, t dbSNP:386352352
1261 1261 a, g dbSNP:780953754
1267 1267 a, c dbSNP:770747549
1271 1271 a, c dbSNP:746506291
1279 1279 c, t dbSNP:756658679
1283 1283 a, c dbSNP:750769824
1291 1291 c, t dbSNP:781602713
1306 1306 c, g dbSNP:757644974
1307 1307 -, c dbSNP:34988264
1312 1312 a, g dbSNP:751673161
1336 1336 c, t dbSNP:764397826
1345 1345 a, g dbSNP:137911238
1351 1351 c, t dbSNP:752866900
1356 1356 c, t dbSNP:765243751
1363 1363 a, g dbSNP:759605652
1366 1366 c, t dbSNP:776566422
1387 1387 c, t dbSNP:770568845
1388 1388 a, g dbSNP:760535486
1403 1403 c, t dbSNP:773941420
1404 1404 a, g dbSNP:763766541
1418 1418 a, t dbSNP:762420208
1424 1424 c, t dbSNP:775116225
1425 1425 c, g dbSNP:35635531
1461 1461 g, t dbSNP:761312324
1477 1477 c, g dbSNP:3729858
1482 1482 a, g dbSNP:772509949
1486 1486 c, t dbSNP:748549700
1498 1498 a, g dbSNP:774370135
1503 1503 a, g dbSNP:779240866
1507 1507 c, t dbSNP:769046142
1509 1509 c, t dbSNP:749362012
1540 1540 c, g, t dbSNP:756149376
1547 1547 a, g dbSNP:750316086
1552 1552 c, t dbSNP:781011594
1555 1555 c, t dbSNP:200573717
1558 1558 a, g dbSNP:149272491
1560 1560 a, g dbSNP:187770246
1561 1561 a, g dbSNP:371711255
1576 1576 c, t dbSNP:145797642
1586 1586 a, c dbSNP:752340563
1589 1589 c, t dbSNP:149832080
1595 1595 c, g dbSNP:373941966
1604 1604 a, g dbSNP:759051524
1606 1606 c, t dbSNP:753333407
1609 1609 a, g dbSNP:370893378
1612 1612 c, t dbSNP:553187237
1621 1621 c, t dbSNP:762472510
1627 1627 c, t dbSNP:775000550
1630 1630 a, g dbSNP:370912699
1643 1643 c, t dbSNP:573921316
1644 1644 a, g dbSNP:377316394
1656 1656 a, g dbSNP:775739774
1660 1660 c, g dbSNP:769948900
1667 1667 a, g dbSNP:745937862
1679 1679 c, t dbSNP:141087932
1682 1682 a, g dbSNP:770850404
1684 1684 c, g dbSNP:746884607
1693 1693 a, g dbSNP:777703093
1698 1698 a, c dbSNP:186700926
1699 1699 c, g dbSNP:747928705
1701 1701 c, g dbSNP:41296312
1702 1702 g, t dbSNP:754638938
1704 1704 a, c dbSNP:574054401
1708 1708 c, t dbSNP:765916882
1710 1710 a, t dbSNP:41296314
1711 1711 a, g dbSNP:368971640
1713 1713 a, g, t dbSNP:763345212
1715 1715 c, t dbSNP:775765231
1718 1718 c, t dbSNP:765484342
1725 1725 c, t dbSNP:759743296
1728 1728 -, tttt dbSNP:777513827
1732 1732 -, ctttttt dbSNP:141786059
1732 1732 -, c dbSNP:758345813
1740 1740 -, t dbSNP:754820234
1740 1740 -, t dbSNP:765052810
1749 1749 g, t dbSNP:540631278
1758 1758 a, g dbSNP:540043364
1778 1778 a, g dbSNP:41296316
1828 1828 a, g dbSNP:546874591
1828 1828 -, g dbSNP:35571116
1884 1884 c, t dbSNP:558194814
1932 1932 c, t dbSNP:11541565
1975 1975 c, t dbSNP:575871334
2033 2033 a, c dbSNP:41296320
2038 2038 c, t dbSNP:770941968
2072 2072 a, c dbSNP:11541564
2090 2090 c, t dbSNP:773096384
2139 2139 a, g dbSNP:41296322
2147 2147 g, t dbSNP:535704731
2189 2189 a, g dbSNP:192062046
2223 2223 a, t dbSNP:41296324
2224 2224 a, t dbSNP:533644410
2273 2273 c, t dbSNP:771557895
2275 2275 c, t dbSNP:374398241
2313 2313 c, t dbSNP:372738854
2365 2365 a, g dbSNP:371294220
2389 2389 a, t dbSNP:751142658
2405 2405 g, t dbSNP:761592242
2444 2444 a, g dbSNP:533264551
2467 2467 -, ctct dbSNP:750815980
2469 2469 -, ct dbSNP:536003350
2474 2474 c, g, t dbSNP:181393862
2476 2476 c, t dbSNP:551104459
2584 2584 a, g dbSNP:533224290
2599 2599 c, t dbSNP:773836816
2603 2603 a, g dbSNP:768206023
2612 2612 c, t dbSNP:189384496
2622 2622 c, t dbSNP:550733152
2636 2636 c, t dbSNP:529114892
2641 2641 c, t dbSNP:138923029
2652 2652 a, g dbSNP:780499415
2658 2658 a, c dbSNP:770356716
2661 2661 a, t dbSNP:540693985
2745 2745 c, t dbSNP:746164515
2771 2771 c, g dbSNP:373483298
2778 2778 -, tcac dbSNP:537314114
2803 2803 a, t dbSNP:781626359
2823 2823 a, g dbSNP:564446879
2852 2852 a, c dbSNP:184594472
2878 2878 -, t dbSNP:41296326
2888 2888 c, t dbSNP:757419024
2894 2894 a, c dbSNP:575879842
2921 2921 a, g dbSNP:41296328
2927 2927 a, c dbSNP:15247
2944 2944 c, t dbSNP:751891973
2976 2976 c, g dbSNP:369471735
3000 3000 a, g dbSNP:181006322
3058 3058 g, t dbSNP:189139001

Target ORF information:

RefSeq Version NM_001304349
Organism Homo sapiens (human)
Definition Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001304349

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTTCACCT TGGGATACCA TGGGGCCGAG GAGTGGGCAT CGGGCGAGAC TTCCCTTGAA 
CCAGCTCTGG AGCAATGCCC TTGCGATTGC CACGGAGAGG AACAGGGCCA GCTGGGGGCC
AAGGTGACAG CTGAGCCCCC CAAGGGCACC TGCTACCCGA GCCCCCGAGG CCTCCATCCC
AGGCCCGGCC TCCCCAGAGA AGGGGCCAGA GGAACAGCCA AATCCCAAGG CTATAAATAC
CTCGGCTGGC GTCGGCCCTG GCCATGGAAT GTCGTGAAAG AATTCTTAGC CAAAGCCAAA
GAAGATTTTC TTAAAAAATG GGAAAGTCCC GCTCAGAACA CAGCCCACTT GGATCAGTTT
GAACGAATCA AGACCCTCGG CACGGGCTCC TTCGGGCGGG TGATGCTGGT GAAACACAAG
GAGACCGGGA ACCACTATGC CATGAAGATC CTCGACAAAC AGAAGGTGGT GAAACTGAAA
CAGATCGAAC ACACCCTGAA TGAAAAGCGC ATCCTGCAAG CTGTCAACTT TCCGTTCCTC
GTCAAACTCG AGTTCTCCTT CAAGGACAAC TCAAACTTAT ACATGGTCAT GGAGTACGTG
CCCGGCGGGG AGATGTTCTC ACACCTACGG CGGATCGGAA GGTTCAGTGA GCCCCATGCC
CGTTTCTACG CGGCCCAGAT CGTCCTGACC TTTGAGTATC TGCACTCGCT GGATCTCATC
TACAGGGACC TGAAGCCGGA GAATCTGCTC ATTGACCAGC AGGGCTACAT TCAGGTGACA
GACTTCGGTT TCGCCAAGCG CGTGAAGGGC CGCACTTGGA CCTTGTGCGG CACCCCTGAG
TACCTGGCCC CTGAGATTAT CCTGAGCAAA GGCTACAACA AGGCCGTGGA CTGGTGGGCC
CTGGGGGTTC TTATCTATGA AATGGCCGCT GGCTACCCGC CCTTCTTCGC AGACCAGCCC
ATCCAGATCT ATGAGAAGAT CGTCTCTGGG AAGGTGCGCT TCCCTTCCCA CTTCAGCTCT
GACTTGAAGG ACCTGCTGCG GAACCTCCTG CAGGTAGATC TCACCAAGCG CTTTGGGAAC
CTCAAGAATG GGGTCAACGA TATCAAGAAC CACAAGTGGT TTGCCACAAC TGACTGGATT
GCCATCTACC AGAGGAAGGT GGAAGCTCCC TTCATACCAA AGTTTAAAGG CCCTGGGGAT
ACGAGTAACT TTGACGACTA TGAGGAAGAA GAAATCCGGG TCTCCATCAA TGAGAAGTGT
GGCAAGGAGT TTTCTGAGTT TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18669
Clone ID Related Accession (Same CDS sequence) NM_002730
Accession Version NM_002730.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1056bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU543573.1 and BC039846.1. This sequence is a reference standard in the RefSeqGene project. On Apr 30, 2004 this sequence version replaced gi:31543437. Summary: This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015]. Transcript Variant: This variant (1) encodes Calpha1 (PMID:21812984). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC108259.1, X07767.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962346 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAGG AGAGCGTGAA AGAATTCTTA 
GCCAAAGCCA AAGAAGATTT TCTTAAAAAA TGGGAAAGTC CCGCTCAGAA CACAGCCCAC
TTGGATCAGT TTGAACGAAT CAAGACCCTC GGCACGGGCT CCTTCGGGCG GGTGATGCTG
GTGAAACACA AGGAGACCGG GAACCACTAT GCCATGAAGA TCCTCGACAA ACAGAAGGTG
GTGAAACTGA AACAGATCGA ACACACCCTG AATGAAAAGC GCATCCTGCA AGCTGTCAAC
TTTCCGTTCC TCGTCAAACT CGAGTTCTCC TTCAAGGACA ACTCAAACTT ATACATGGTC
ATGGAGTACG TGCCCGGCGG GGAGATGTTC TCACACCTAC GGCGGATCGG AAGGTTCAGT
GAGCCCCATG CCCGTTTCTA CGCGGCCCAG ATCGTCCTGA CCTTTGAGTA TCTGCACTCG
CTGGATCTCA TCTACAGGGA CCTGAAGCCG GAGAATCTGC TCATTGACCA GCAGGGCTAC
ATTCAGGTGA CAGACTTCGG TTTCGCCAAG CGCGTGAAGG GCCGCACTTG GACCTTGTGC
GGCACCCCTG AGTACCTGGC CCCTGAGATT ATCCTGAGCA AAGGCTACAA CAAGGCCGTG
GACTGGTGGG CCCTGGGGGT TCTTATCTAT GAAATGGCCG CTGGCTACCC GCCCTTCTTC
GCAGACCAGC CCATCCAGAT CTATGAGAAG ATCGTCTCTG GGAAGGTGCG CTTCCCTTCC
CACTTCAGCT CTGACTTGAA GGACCTGCTG CGGAACCTCC TGCAGGTAGA TCTCACCAAG
CGCTTTGGGA ACCTCAAGAA TGGGGTCAAC GATATCAAGA ACCACAAGTG GTTTGCCACA
ACTGACTGGA TTGCCATCTA CCAGAGGAAG GTGGAAGCTC CCTTCATACC AAAGTTTAAA
GGCCCTGGGG ATACGAGTAA CTTTGACGAC TATGAGGAAG AAGAAATCCG GGTCTCCATC
AATGAGAAGT GTGGCAAGGA GTTTTCTGAG TTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002721.1
CDS201..1256
Misc Feature(1)207..209(+)
Misc Feature(2)231..233(+)
Misc Feature(3)231..233(+)
Misc Feature(4)324..1193(+)
Misc Feature(5)330..1094(+)
Misc Feature(6)345..347(+)
Misc Feature(7)348..1184(+)
Misc Feature(8)351..1193(+)
Misc Feature(9)354..1193(+)
Misc Feature(10)360..1193(+)
Misc Feature(11)618..620(+)
Misc Feature(12)618..620(+)
Misc Feature(13)750..812(+)
Misc Feature(14)786..788(+)
Misc Feature(15)786..788(+)
Misc Feature(16)792..794(+)
Misc Feature(17)792..794(+)
Misc Feature(18)804..806(+)
Misc Feature(19)804..806(+)
Misc Feature(20)1191..1193(+)
Misc Feature(21)1215..1217(+)
Misc Feature(22)1215..1217(+)
Exon (1)1..246
Gene:PRKACA
Gene Synonym:
Exon (2)247..308
Gene:PRKACA
Gene Synonym:
Exon (3)309..437
Gene:PRKACA
Gene Synonym:
Exon (4)438..536
Gene:PRKACA
Gene Synonym:
Exon (5)537..619
Gene:PRKACA
Gene Synonym:
Exon (6)620..746
Gene:PRKACA
Gene Synonym:
Exon (7)747..842
Gene:PRKACA
Gene Synonym:
Exon (8)843..965
Gene:PRKACA
Gene Synonym:
Exon (9)966..1130
Gene:PRKACA
Gene Synonym:
Exon (10)1131..2680
Gene:PRKACA
Gene Synonym:
Translation
Position Chain Variation Link
20 20 c, t dbSNP:143276259
39 39 a, t dbSNP:578162771
59 59 c, g dbSNP:563054475
197 197 -, cgc dbSNP:781415461
244 244 a, g dbSNP:546895409
290 290 a, g dbSNP:771393106
295 295 a, c dbSNP:760954437
302 302 c, t dbSNP:56105247
303 303 a, g dbSNP:142045517
307 307 a, g dbSNP:748193038
311 311 c, t dbSNP:767936415
313 313 c, t dbSNP:762166548
316 316 c, g dbSNP:774495964
321 321 g, t dbSNP:56029020
336 336 a, c dbSNP:764242716
337 337 a, g dbSNP:56085217
349 349 a, c, t dbSNP:112360106
350 350 c, t dbSNP:775474906
356 356 a, c, g dbSNP:745853839
365 365 c, t dbSNP:776568028
368 368 a, g, t dbSNP:373468300
370 370 a, g dbSNP:746814323
385 385 a, g dbSNP:777520540
390 390 -, aag dbSNP:762588021
391 391 a, g dbSNP:758020326
394 394 a, g dbSNP:747669394
396 396 -, acc dbSNP:752551387
396 396 a, c, g dbSNP:201769960
398 398 c, g, t dbSNP:768020526
399 399 c, g dbSNP:757565596
408 408 c, t dbSNP:201225070
415 415 c, t dbSNP:764342858
425 425 c, t dbSNP:763136554
426 426 a, g dbSNP:369286962
432 432 c, t dbSNP:765305631
440 440 a, g dbSNP:773054959
458 458 c, t dbSNP:368239098
462 462 c, t dbSNP:376284068
466 466 c, g dbSNP:773821844
471 471 a, c dbSNP:768324869
480 480 c, t dbSNP:748763072
481 481 a, g dbSNP:779586629
491 491 a, g dbSNP:371232485
505 505 c, g dbSNP:747585702
506 506 a, g dbSNP:202032822
510 510 c, t dbSNP:758631881
512 512 c, t dbSNP:753127913
518 518 a, g dbSNP:376182047
521 521 c, g, t dbSNP:148811989
522 522 c, g dbSNP:766631376
541 541 a, g dbSNP:369058901
553 553 a, g dbSNP:775066482
554 554 c, t dbSNP:376915447
555 555 a, g dbSNP:758933458
569 569 c, t dbSNP:201100703
575 575 c, t dbSNP:772438487
581 581 a, g dbSNP:78098302
600 600 c, t dbSNP:775000520
603 603 c, t dbSNP:769081071
604 604 a, g dbSNP:749688339
608 608 c, t dbSNP:141320866
609 609 a, g dbSNP:148280386
613 613 c, g dbSNP:756398151
626 626 c, t dbSNP:777648038
629 629 a, t dbSNP:758375271
641 641 c, t dbSNP:558688172
643 643 a, c dbSNP:778829250
644 644 c, g dbSNP:754730934
650 650 a, g dbSNP:753469180
653 653 c, t dbSNP:145751355
661 661 a, c dbSNP:765966838
662 662 c, g dbSNP:760188119
666 666 a, g dbSNP:749992796
680 680 a, g dbSNP:764675218
710 710 a, g dbSNP:763611702
716 716 c, t dbSNP:775990456
717 717 a, c dbSNP:537279998
719 719 g, t dbSNP:759833330
724 724 c, t dbSNP:776865039
731 731 g, t dbSNP:771089857
732 732 a, c dbSNP:201595411
740 740 c, t dbSNP:745485648
741 741 a, g dbSNP:142007512
743 743 a, t dbSNP:748153891
749 749 a, c, g dbSNP:746473377
758 758 c, t dbSNP:761035852
760 760 g, t dbSNP:11541563
764 764 c, t dbSNP:773370977
765 765 a, g dbSNP:767755931
771 771 c, t dbSNP:761845520
772 772 a, g dbSNP:774340605
773 773 c, t dbSNP:768624241
774 774 a, g dbSNP:372210586
784 784 a, g dbSNP:777156218
795 795 c, t dbSNP:775500643
799 799 -, gtg dbSNP:724160013
800 800 c, t dbSNP:769653802
809 809 c, t dbSNP:745681054
817 817 g, t dbSNP:386352352
827 827 a, g dbSNP:780953754
833 833 a, c dbSNP:770747549
837 837 a, c dbSNP:746506291
845 845 c, t dbSNP:756658679
849 849 a, c dbSNP:750769824
857 857 c, t dbSNP:781602713
872 872 c, g dbSNP:757644974
873 873 -, c dbSNP:34988264
878 878 a, g dbSNP:751673161
902 902 c, t dbSNP:764397826
911 911 a, g dbSNP:137911238
917 917 c, t dbSNP:752866900
922 922 c, t dbSNP:765243751
929 929 a, g dbSNP:759605652
932 932 c, t dbSNP:776566422
953 953 c, t dbSNP:770568845
954 954 a, g dbSNP:760535486
969 969 c, t dbSNP:773941420
970 970 a, g dbSNP:763766541
984 984 a, t dbSNP:762420208
990 990 c, t dbSNP:775116225
991 991 c, g dbSNP:35635531
1027 1027 g, t dbSNP:761312324
1043 1043 c, g dbSNP:3729858
1048 1048 a, g dbSNP:772509949
1052 1052 c, t dbSNP:748549700
1064 1064 a, g dbSNP:774370135
1069 1069 a, g dbSNP:779240866
1073 1073 c, t dbSNP:769046142
1075 1075 c, t dbSNP:749362012
1106 1106 c, g, t dbSNP:756149376
1113 1113 a, g dbSNP:750316086
1118 1118 c, t dbSNP:781011594
1121 1121 c, t dbSNP:200573717
1124 1124 a, g dbSNP:149272491
1126 1126 a, g dbSNP:187770246
1127 1127 a, g dbSNP:371711255
1142 1142 c, t dbSNP:145797642
1152 1152 a, c dbSNP:752340563
1155 1155 c, t dbSNP:149832080
1161 1161 c, g dbSNP:373941966
1170 1170 a, g dbSNP:759051524
1172 1172 c, t dbSNP:753333407
1175 1175 a, g dbSNP:370893378
1178 1178 c, t dbSNP:553187237
1187 1187 c, t dbSNP:762472510
1193 1193 c, t dbSNP:775000550
1196 1196 a, g dbSNP:370912699
1209 1209 c, t dbSNP:573921316
1210 1210 a, g dbSNP:377316394
1222 1222 a, g dbSNP:775739774
1226 1226 c, g dbSNP:769948900
1233 1233 a, g dbSNP:745937862
1245 1245 c, t dbSNP:141087932
1248 1248 a, g dbSNP:770850404
1250 1250 c, g dbSNP:746884607
1259 1259 a, g dbSNP:777703093
1264 1264 a, c dbSNP:186700926
1265 1265 c, g dbSNP:747928705
1267 1267 c, g dbSNP:41296312
1268 1268 g, t dbSNP:754638938
1270 1270 a, c dbSNP:574054401
1274 1274 c, t dbSNP:765916882
1276 1276 a, t dbSNP:41296314
1277 1277 a, g dbSNP:368971640
1279 1279 a, g, t dbSNP:763345212
1281 1281 c, t dbSNP:775765231
1284 1284 c, t dbSNP:765484342
1291 1291 c, t dbSNP:759743296
1294 1294 -, tttt dbSNP:777513827
1298 1298 -, ctttttt dbSNP:141786059
1298 1298 -, c dbSNP:758345813
1306 1306 -, t dbSNP:754820234
1306 1306 -, t dbSNP:765052810
1315 1315 g, t dbSNP:540631278
1324 1324 a, g dbSNP:540043364
1344 1344 a, g dbSNP:41296316
1394 1394 a, g dbSNP:546874591
1394 1394 -, g dbSNP:35571116
1450 1450 c, t dbSNP:558194814
1498 1498 c, t dbSNP:11541565
1541 1541 c, t dbSNP:575871334
1599 1599 a, c dbSNP:41296320
1604 1604 c, t dbSNP:770941968
1638 1638 a, c dbSNP:11541564
1656 1656 c, t dbSNP:773096384
1705 1705 a, g dbSNP:41296322
1713 1713 g, t dbSNP:535704731
1755 1755 a, g dbSNP:192062046
1789 1789 a, t dbSNP:41296324
1790 1790 a, t dbSNP:533644410
1839 1839 c, t dbSNP:771557895
1841 1841 c, t dbSNP:374398241
1879 1879 c, t dbSNP:372738854
1931 1931 a, g dbSNP:371294220
1955 1955 a, t dbSNP:751142658
1971 1971 g, t dbSNP:761592242
2010 2010 a, g dbSNP:533264551
2033 2033 -, ctct dbSNP:750815980
2035 2035 -, ct dbSNP:536003350
2040 2040 c, g, t dbSNP:181393862
2042 2042 c, t dbSNP:551104459
2150 2150 a, g dbSNP:533224290
2165 2165 c, t dbSNP:773836816
2169 2169 a, g dbSNP:768206023
2178 2178 c, t dbSNP:189384496
2188 2188 c, t dbSNP:550733152
2202 2202 c, t dbSNP:529114892
2207 2207 c, t dbSNP:138923029
2218 2218 a, g dbSNP:780499415
2224 2224 a, c dbSNP:770356716
2227 2227 a, t dbSNP:540693985
2311 2311 c, t dbSNP:746164515
2337 2337 c, g dbSNP:373483298
2344 2344 -, tcac dbSNP:537314114
2369 2369 a, t dbSNP:781626359
2389 2389 a, g dbSNP:564446879
2418 2418 a, c dbSNP:184594472
2444 2444 -, t dbSNP:41296326
2454 2454 c, t dbSNP:757419024
2460 2460 a, c dbSNP:575879842
2487 2487 a, g dbSNP:41296328
2493 2493 a, c dbSNP:15247
2510 2510 c, t dbSNP:751891973
2542 2542 c, g dbSNP:369471735
2566 2566 a, g dbSNP:181006322
2624 2624 g, t dbSNP:189139001

Target ORF information:

RefSeq Version NM_002730
Organism Homo sapiens (human)
Definition Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002730

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAGG AGAGCGTGAA AGAATTCTTA 
GCCAAAGCCA AAGAAGATTT TCTTAAAAAA TGGGAAAGTC CCGCTCAGAA CACAGCCCAC
TTGGATCAGT TTGAACGAAT CAAGACCCTC GGCACGGGCT CCTTCGGGCG GGTGATGCTG
GTGAAACACA AGGAGACCGG GAACCACTAT GCCATGAAGA TCCTCGACAA ACAGAAGGTG
GTGAAACTGA AACAGATCGA ACACACCCTG AATGAAAAGC GCATCCTGCA AGCTGTCAAC
TTTCCGTTCC TCGTCAAACT CGAGTTCTCC TTCAAGGACA ACTCAAACTT ATACATGGTC
ATGGAGTACG TGCCCGGCGG GGAGATGTTC TCACACCTAC GGCGGATCGG AAGGTTCAGT
GAGCCCCATG CCCGTTTCTA CGCGGCCCAG ATCGTCCTGA CCTTTGAGTA TCTGCACTCG
CTGGATCTCA TCTACAGGGA CCTGAAGCCG GAGAATCTGC TCATTGACCA GCAGGGCTAC
ATTCAGGTGA CAGACTTCGG TTTCGCCAAG CGCGTGAAGG GCCGCACTTG GACCTTGTGC
GGCACCCCTG AGTACCTGGC CCCTGAGATT ATCCTGAGCA AAGGCTACAA CAAGGCCGTG
GACTGGTGGG CCCTGGGGGT TCTTATCTAT GAAATGGCCG CTGGCTACCC GCCCTTCTTC
GCAGACCAGC CCATCCAGAT CTATGAGAAG ATCGTCTCTG GGAAGGTGCG CTTCCCTTCC
CACTTCAGCT CTGACTTGAA GGACCTGCTG CGGAACCTCC TGCAGGTAGA TCTCACCAAG
CGCTTTGGGA ACCTCAAGAA TGGGGTCAAC GATATCAAGA ACCACAAGTG GTTTGCCACA
ACTGACTGGA TTGCCATCTA CCAGAGGAAG GTGGAAGCTC CCTTCATACC AAAGTTTAAA
GGCCCTGGGG ATACGAGTAA CTTTGACGAC TATGAGGAAG AAGAAATCCG GGTCTCCATC
AATGAGAAGT GTGGCAAGGA GTTTTCTGAG TTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Recurrent activating mutation in PRKACA in cortisol-producing adrenal tumors
Nat. Genet. 46 (6), 613-617 (2014)
Goh G, Scholl UI, Healy JM, Choi M, Prasad ML, Nelson-Williams C, Kunstman JW, Korah R, Suttorp AC, Dietrich D, Haase M, Willenberg HS, Stalberg P, Hellman P, Akerstrom G, Bjorklund P, Carling T and Lifton RP.


book

Recurrent somatic mutations underlie corticotropin-independent Cushing's syndrome
Science 344 (6186), 917-920 (2014)
Sato Y, Maekawa S, Ishii R, Sanada M, Morikawa T, Shiraishi Y, Yoshida K, Nagata Y, Sato-Otsubo A, Yoshizato T, Suzuki H, Shiozawa Y, Kataoka K, Kon A, Aoki K, Chiba K, Tanaka H, Kume H, Miyano S, Fukayama M, Nureki O, Homma Y and Ogawa S.


book

Activating hotspot L205R mutation in PRKACA and adrenal Cushing's syndrome
Science 344 (6186), 913-917 (2014)
Cao Y, He M, Gao Z, Peng Y, Li Y, Li L, Zhou W, Li X, Zhong X, Lei Y, Su T, Wang H, Jiang Y, Yang L, Wei W, Yang X, Jiang X, Liu L, He J, Ye J, Wei Q, Li Y, Wang W, Wang J and Ning G.


book

Constitutive activation of PKA catalytic subunit in adrenal Cushing's syndrome
N. Engl. J. Med. 370 (11), 1019-1028 (2014)
Beuschlein F, Fassnacht M, Assie G, Calebiro D, Stratakis CA, Osswald A, Ronchi CL, Wieland T, Sbiera S, Faucz FR, Schaak K, Schmittfull A, Schwarzmayr T, Barreau O, Vezzosi D, Rizk-Rabin M, Zabel U, Szarek E, Salpea P, Forlino A, Vetro A, Zuffardi O, Kisker C, Diener S, Meitinger T, Lohse MJ, Reincke M, Bertherat J, Strom TM and Allolio B.


book

The unique catalytic subunit of sperm cAMP-dependent protein kinase is the product of an alternative Calpha mRNA expressed specifically in spermatogenic cells
Mol. Biol. Cell 11 (9), 3031-3044 (2000)
Agustin JT, Wilkerson CG and Witman GB.


book

A novel isoform of human cyclic 3',5'-adenosine monophosphate-dependent protein kinase, c alpha-s, localizes to sperm midpiece
Biol. Reprod. 63 (2), 607-611 (2000)
Reinton N, Orstavik S, Haugen TB, Jahnsen T, Tasken K and Skalhegg BS.


book

Expression of a nonmyristylated variant of the catalytic subunit of protein kinase A during male germ-cell development
Proc. Natl. Acad. Sci. U.S.A. 97 (12), 6433-6438 (2000)
Desseyn JL, Burton KA and McKnight GS.


book

A cDNA clone encoding human cAMP-dependent protein kinase catalytic subunit C alpha
Nucleic Acids Res. 16 (16), 8189-8190 (1988)
Maldonado F and Hanks SK.


book

Sequence of two phosphorylated sites in the catalytic subunit of bovine cardiac muscle adenosine 3':5'-monophosphate-dependent protein kinase
J. Biol. Chem. 254 (14), 6211-6214 (1979)
Shoji,S., Titani,K., Demaille,J.G. and Fischer,E.H.


book

Direct phosphorylation of brain tyrosine hydroxylase by cyclic AMP-dependent protein kinase: mechanism of enzyme activation
Proc. Natl. Acad. Sci. U.S.A. 75 (10), 4744-4748 (1978)
Joh,T.H., Park,D.H. and Reis,D.J.


 
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