×

POLR3B cDNA ORF clone, Homo sapiens (human)

Gene Symbol POLR3B
Entrez Gene ID 55703
Full Name polymerase (RNA) III (DNA directed) polypeptide B
Synonyms C128, HLD8, INMAP, RPC2
General protein information
Preferred Names
DNA-directed RNA polymerase III subunit RPC2
Names
DNA-directed RNA polymerase III subunit RPC2
RNA polymerase III subunit C2
DNA-directed RNA polymerase III subunit B
DNA-directed RNA polymerase III 127.6 kDa polypeptide
NP_001154180.1
NP_060552.4
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12q23.3

Summary This gene encodes the second largest subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The largest subunit and the encoded protein form the catalytic center of RNA polymerase III. Mutations in this gene are a cause of hypomyelinating leukodystrophy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]. lac of sum
Disorder MIM:

614366


mRNA and Protein(s)

mRNA Protein Name
NM_001160708 NP_001154180 DNA-directed RNA polymerase III subunit RPC2 isoform 2
NM_018082 NP_060552 DNA-directed RNA polymerase III subunit RPC2 isoform 1



Homo sapiens (human) POLR3B NP_060552.4
Pan troglodytes (chimpanzee) POLR3B XP_001161567.1
Macaca mulatta (Rhesus monkey) POLR3B XP_001099515.1
Canis lupus familiaris (dog) POLR3B XP_531760.2
Bos taurus (cattle) POLR3B NP_001179718.1
Mus musculus (house mouse) Polr3b NP_081699.1
Rattus norvegicus (Norway rat) Polr3b NP_001178807.1
Gallus gallus (chicken) POLR3B XP_416307.3
Danio rerio (zebrafish) polr3b XP_690811.2
Drosophila melanogaster (fruit fly) RpIII128 NP_523706.1
Caenorhabditis elegans F09F7.3 NP_498192.2
Arabidopsis thaliana (thale cress) NRPC2 NP_199327.4
Xenopus (Silurana) tropicalis (western clawed frog) polr3b NP_001121521.1


Related articles in PubMed

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POLR3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu22699
NM_001160708 Homo sapiens polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00
OHu27279
NM_018082 Homo sapiens polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22699
Clone ID Related Accession (Same CDS sequence) NM_001160708
Accession Version NM_001160708.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product DNA-directed RNA polymerase III subunit RPC2 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB094040.1, AK122713.1, AC078992.23 and BC033724.2. Transcript Variant: This variant (2) differs in the 5' UTR and initiates translation at an in-frame downstream AUG, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122713.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGAAAGCCA ATGAAAAGGT TACAAGTGAC GCTGACCCTA TGTGGTACTT AAAATATCTT 
AATATCTATG TTGGGCTTCC TGATGTTGAA GAAAGCTTCA ATGTAACTAG ACCAGTGTCC
CCTCATGAGT GCCGTTTGAG AGACATGACA TACTCTGCCC CTATTACAGT GGATATTGAA
TATACCCGAG GCAGCCAGAG GATCATCCGC AATGCCTTAC CTATCGGCAG AATGCCCATA
ATGCTACGTA GTTCAAACTG TGTTCTTACA GGAAAAACGC CAGCAGAATT TGCCAAACTG
AACGAATGTC CCTTAGATCC AGGTGGCTAC TTCATTGTTA AAGGAGTAGA AAAAGTTATT
CTTATCCAAG AGCAGCTGTC TAAGAACAGG ATCATCGTGG AGGCTGATAG AAAAGGGGCT
GTTGGAGCTT CAGTTACCAG CTCTACCCAT GAGAAAAAAA GCAGAACCAA TATGGCTGTG
AAACAAGGAC GATTTTATTT GAGGCATAAT ACTTTGTCAG AAGATATACC CATTGTCATC
ATATTTAAGG CCATGGGTGT TGAGAGTGAC CAGGAAATTG TGCAGATGAT TGGAACAGAG
GAGCACGTGA TGGCTGCATT TGGGCCCAGT CTGGAAGAGT GCCAGAAAGC TCAGATTTTC
ACACAGATGC AGGCATTAAA ATATATAGGG AACAAAGTAA GAAGGCAAAG GATGTGGGGA
GGTGGACCAA AGAAAACCAA AATAGAAGAA GCAAGAGAGC TCCTGGCTTC CACCATTCTG
ACCCATGTCC CAGTTAAGGA ATTCAATTTC CGAGCCAAAT GTATCTATAC TGCAGTGATG
GTGCGAAGAG TTATTCTGGC CCAAGGAGAT AATAAAGTTG ACGACAGAGA TTATTATGGT
AACAAGCGAC TGGAATTGGC AGGACAGCTT TTATCTCTTC TTTTTGAAGA CTTGTTCAAA
AAATTTAATT CTGAAATGAA AAAGATTGCC GACCAGGTGA TTCCTAAGCA AAGAGCAGCC
CAGTTTGATG TTGTCAAACA CATGCGCCAA GACCAGATCA CCAATGGCAT GGTGAATGCT
ATTTCTACCG GAAATTGGTC TTTAAAGAGA TTTAAAATGG ACCGCCAGGG TGTAACCCAA
GTGCTGTCTC GCTTGTCATA TATATCCGCA CTGGGCATGA TGACAAGAAT CTCTTCCCAG
TTTGAAAAAA CGAGAAAAGT GAGTGGTCCT CGCTCCCTCC AGCCATCTCA GTGGGGAATG
CTGTGTCCTT CGGACACTCC TGAAGGAGAG GCATGTGGTT TGGTTAAAAA CTTGGCCCTT
ATGACACACA TCACAACTGA TATGGAAGAT GGACCCATTG TTAAATTAGC CAGTAACTTG
GGAGTAGAAG ATGTGAATTT ATTATGTGGG GAAGAGCTCT CTTACCCAAA TGTGTTTCTT
GTCTTTCTTA ATGGTAACAT CTTAGGTGTC ATTCGAGACC ACAAAAAGCT AGTGAATACA
TTTCGACTCA TGAGAAGAGC AGGATATATC AATGAATTTG TTTCCATCTC AACAAATCTT
ACAGATCGAT GTGTCTATAT TTCTTCTGAT GGGGGAAGGC TATGCAGACC CTACATAATT
GTCAAGAAAC AGAAGCCAGC AGTCACAAAT AAACATATGG AAGAGCTGGC CCAAGGGTAC
AGGAATTTTG AAGATTTCTT ACATGAGAGT CTGGTTGAAT ATTTAGATGT GAATGAAGAA
AATGATTGTA ACATTGCACT GTACGAACAC ACAATTAATA AAGACACCAC CCACTTGGAG
ATTGAACCCT TCACTCTTCT CGGCGTGTGT GCTGGACTTA TCCCATACCC TCACCATAAC
CAGTCACCGA GAAACACTTA TCAGTGTGCC ATGGGGAAAC AAGCCATGGG TACTATAGGA
TACAACCAGC GAAACAGAAT TGATACTCTC ATGTATCTAC TAGCATATCC ACAAAAACCC
ATGGTTAAGA CAAAAACCAT TGAATTGATA GAATTTGAGA AACTGCCAGC TGGACAGAAT
GCAACAGTTG CTGTGATGAG CTATAGTGGC TATGATATTG AAGATGCTCT TGTTTTAAAC
AAGGCCTCTT TAGACAGAGG CTTTGGGCGT TGCCTTGTAT ATAAAAATGC TAAATGTACG
TTGAAACGAT ACACCAATCA GACTTTTGAT AAAGTGATGG GGCCCATGTT GGATGCTGCT
ACAAGGAAAC CTATCTGGCG ACATGAAATC TTAGATGCAG ATGGTATTTG TTCTCCAGGT
GAGAAAGTAG AAAACAAACA AGTGCTTGTA AATAAGTCCA TGCCCACAGT GACTCAGATT
CCTTTGGAAG GAAGTAATGT ACCACAGCAA CCACAGTACA AAGATGTACC CATAACCTAC
AAAGGAGCAA CAGACTCATA TATTGAAAAA GTGATGATAT CTTCAAATGC TGAAGATGCT
TTTCTGATCA AAATGCTGCT GAGACAGACA AGGCGTCCAG AAATTGGAGA CAAATTCAGC
AGTCGTCATG GGCAAAAAGG TGTTTGTGGC TTGATCGTCC CCCAGGAAGA CATGCCATTT
TGTGATTCTG GCATCTGTCC GGACATCATC ATGAACCCAC ACGGCTTCCC ATCACGAATG
ACGGTGGGGA AGCTCATTGA GCTGCTGGCT GGCAAGGCCG GTGTGCTGGA CGGCAGATTC
CACTACGGCA CTGCGTTTGG AGGCAGTAAA GTGAAGGATG TGTGTGAGGA CCTCGTTCGC
CATGGTTATA ACTACTTGGG GAAAGACTAT GTTACATCCG GCATCACAGG TGAGCCCTTA
GAAGCATACA TCTATTTTGG CCCCGTGTAC TATCAGAAGC TGAAACACAT GGTGCTAGAT
AAAATGCATG CCCGGGCCCG GGGCCCACGA GCCGTCCTTA CCAGGCAACC CACTGAAGGA
CGGTCTCGTG ATGGTGGCTT GCGTCTCGGG GAAATGGAAC GTGACTGTTT AATCGGTTAT
GGAGCCAGTA TGCTTTTGCT AGAGAGACTA ATGATTTCAA GTGATGCCTT TGAGGTTGAT
GTCTGTGGGC AGTGTGGACT TCTGGGGTAT TCTGGCTGGT GCCATTACTG CAAGTCATCC
TGCCACGTGT CTTCCCTCCG TATTCCGTAT GCCTGCAAGC TGCTCTTCCA GGAACTACAG
TCTATGAACA TCATCCCCAG GTTAAAACTG TCCAAGTACA ATGAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001154180.1
CDS244..3471
Misc Feature(1)139..141(+)
Misc Feature(2)244..3450(+)
Misc Feature(3)292..3456(+)
Misc Feature(4)382..2529(+)
Misc Feature(5)1468..3450(+)
Misc Feature(6)1684..1869(+)
Misc Feature(7)1930..2052(+)
Misc Feature(8)2215..3081(+)
Misc Feature(9)2314..3048(+)
Misc Feature(10)2356..3072(+)
Exon (1)1..141
Gene:POLR3B
Gene Synonym:
Exon (2)142..174
Gene:POLR3B
Gene Synonym:
Exon (3)175..231
Gene:POLR3B
Gene Synonym:
Exon (4)232..296
Gene:POLR3B
Gene Synonym:
Exon (5)297..372
Gene:POLR3B
Gene Synonym:
Exon (6)373..473
Gene:POLR3B
Gene Synonym:
Exon (7)474..565
Gene:POLR3B
Gene Synonym:
Exon (8)566..683
Gene:POLR3B
Gene Synonym:
Exon (9)684..792
Gene:POLR3B
Gene Synonym:
Exon (10)793..915
Gene:POLR3B
Gene Synonym:
Exon (11)916..1035
Gene:POLR3B
Gene Synonym:
Exon (12)1036..1170
Gene:POLR3B
Gene Synonym:
Exon (13)1171..1332
Gene:POLR3B
Gene Synonym:
Exon (14)1333..1533
Gene:POLR3B
Gene Synonym:
Exon (15)1534..1696
Gene:POLR3B
Gene Synonym:
Exon (16)1697..1850
Gene:POLR3B
Gene Synonym:
Exon (17)1851..1925
Gene:POLR3B
Gene Synonym:
Exon (18)1926..2024
Gene:POLR3B
Gene Synonym:
Exon (19)2025..2152
Gene:POLR3B
Gene Synonym:
Exon (20)2153..2362
Gene:POLR3B
Gene Synonym:
Exon (21)2363..2521
Gene:POLR3B
Gene Synonym:
Exon (22)2522..2639
Gene:POLR3B
Gene Synonym:
Exon (23)2640..2782
Gene:POLR3B
Gene Synonym:
Exon (24)2783..2886
Gene:POLR3B
Gene Synonym:
Exon (25)2887..3053
Gene:POLR3B
Gene Synonym:
Exon (26)3054..3167
Gene:POLR3B
Gene Synonym:
Exon (27)3168..3341
Gene:POLR3B
Gene Synonym:
Exon (28)3342..4120
Gene:POLR3B
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001160708
Organism Homo sapiens (human)
Definition Homo sapiens polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001160708

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
ATGAAAGCCA ATGAAAAGGT TACAAGTGAC GCTGACCCTA TGTGGTACTT AAAATATCTT 
AATATCTATG TTGGGCTTCC TGATGTTGAA GAAAGCTTCA ATGTAACTAG ACCAGTGTCC
CCTCATGAGT GCCGTTTGAG AGACATGACA TACTCTGCCC CTATTACAGT GGATATTGAA
TATACCCGAG GCAGCCAGAG GATCATCCGC AATGCCTTAC CTATCGGCAG AATGCCCATA
ATGCTACGTA GTTCAAACTG TGTTCTTACA GGAAAAACGC CAGCAGAATT TGCCAAACTG
AACGAATGTC CCTTAGATCC AGGTGGCTAC TTCATTGTTA AAGGAGTAGA AAAAGTTATT
CTTATCCAAG AGCAGCTGTC TAAGAACAGG ATCATCGTGG AGGCTGATAG AAAAGGGGCT
GTTGGAGCTT CAGTTACCAG CTCTACCCAT GAGAAAAAAA GCAGAACCAA TATGGCTGTG
AAACAAGGAC GATTTTATTT GAGGCATAAT ACTTTGTCAG AAGATATACC CATTGTCATC
ATATTTAAGG CCATGGGTGT TGAGAGTGAC CAGGAAATTG TGCAGATGAT TGGAACAGAG
GAGCACGTGA TGGCTGCATT TGGGCCCAGT CTGGAAGAGT GCCAGAAAGC TCAGATTTTC
ACACAGATGC AGGCATTAAA ATATATAGGG AACAAAGTAA GAAGGCAAAG GATGTGGGGA
GGTGGACCAA AGAAAACCAA AATAGAAGAA GCAAGAGAGC TCCTGGCTTC CACCATTCTG
ACCCATGTCC CAGTTAAGGA ATTCAATTTC CGAGCCAAAT GTATCTATAC TGCAGTGATG
GTGCGAAGAG TTATTCTGGC CCAAGGAGAT AATAAAGTTG ACGACAGAGA TTATTATGGT
AACAAGCGAC TGGAATTGGC AGGACAGCTT TTATCTCTTC TTTTTGAAGA CTTGTTCAAA
AAATTTAATT CTGAAATGAA AAAGATTGCC GACCAGGTGA TTCCTAAGCA AAGAGCAGCC
CAGTTTGATG TTGTCAAACA CATGCGCCAA GACCAGATCA CCAATGGCAT GGTGAATGCT
ATTTCTACCG GAAATTGGTC TTTAAAGAGA TTTAAAATGG ACCGCCAGGG TGTAACCCAA
GTGCTGTCTC GCTTGTCATA TATATCCGCA CTGGGCATGA TGACAAGAAT CTCTTCCCAG
TTTGAAAAAA CGAGAAAAGT GAGTGGTCCT CGCTCCCTCC AGCCATCTCA GTGGGGAATG
CTGTGTCCTT CGGACACTCC TGAAGGAGAG GCATGTGGTT TGGTTAAAAA CTTGGCCCTT
ATGACACACA TCACAACTGA TATGGAAGAT GGACCCATTG TTAAATTAGC CAGTAACTTG
GGAGTAGAAG ATGTGAATTT ATTATGTGGG GAAGAGCTCT CTTACCCAAA TGTGTTTCTT
GTCTTTCTTA ATGGTAACAT CTTAGGTGTC ATTCGAGACC ACAAAAAGCT AGTGAATACA
TTTCGACTCA TGAGAAGAGC AGGATATATC AATGAATTTG TTTCCATCTC AACAAATCTT
ACAGATCGAT GTGTCTATAT TTCTTCTGAT GGGGGAAGGC TATGCAGACC CTACATAATT
GTCAAGAAAC AGAAGCCAGC AGTCACAAAT AAACATATGG AAGAGCTGGC CCAAGGGTAC
AGGAATTTTG AAGATTTCTT ACATGAGAGT CTGGTTGAAT ATTTAGATGT GAATGAAGAA
AATGATTGTA ACATTGCACT GTACGAACAC ACAATTAATA AAGACACCAC CCACTTGGAG
ATTGAACCCT TCACTCTTCT CGGCGTGTGT GCTGGACTTA TCCCATACCC TCACCATAAC
CAGTCACCGA GAAACACTTA TCAGTGTGCC ATGGGGAAAC AAGCCATGGG TACTATAGGA
TACAACCAGC GAAACAGAAT TGATACTCTC ATGTATCTAC TAGCATATCC ACAAAAACCC
ATGGTTAAGA CAAAAACCAT TGAATTGATA GAATTTGAGA AACTGCCAGC TGGACAGAAT
GCAACAGTTG CTGTGATGAG CTATAGTGGC TATGATATTG AAGATGCTCT TGTTTTAAAC
AAGGCCTCTT TAGACAGAGG CTTTGGGCGT TGCCTTGTAT ATAAAAATGC TAAATGTACG
TTGAAACGAT ACACCAATCA GACTTTTGAT AAAGTGATGG GGCCCATGTT GGATGCTGCT
ACAAGGAAAC CTATCTGGCG ACATGAAATC TTAGATGCAG ATGGTATTTG TTCTCCAGGT
GAGAAAGTAG AAAACAAACA AGTGCTTGTA AATAAGTCCA TGCCCACAGT GACTCAGATT
CCTTTGGAAG GAAGTAATGT ACCACAGCAA CCACAGTACA AAGATGTACC CATAACCTAC
AAAGGAGCAA CAGACTCATA TATTGAAAAA GTGATGATAT CTTCAAATGC TGAAGATGCT
TTTCTGATCA AAATGCTGCT GAGACAGACA AGGCGTCCAG AAATTGGAGA CAAATTCAGC
AGTCGTCATG GGCAAAAAGG TGTTTGTGGC TTGATCGTCC CCCAGGAAGA CATGCCATTT
TGTGATTCTG GCATCTGTCC GGACATCATC ATGAACCCAC ACGGCTTCCC ATCACGAATG
ACGGTGGGGA AGCTCATTGA GCTGCTGGCT GGCAAGGCCG GTGTGCTGGA CGGCAGATTC
CACTACGGCA CTGCGTTTGG AGGCAGTAAA GTGAAGGATG TGTGTGAGGA CCTCGTTCGC
CATGGTTATA ACTACTTGGG GAAAGACTAT GTTACATCCG GCATCACAGG TGAGCCCTTA
GAAGCATACA TCTATTTTGG CCCCGTGTAC TATCAGAAGC TGAAACACAT GGTGCTAGAT
AAAATGCATG CCCGGGCCCG GGGCCCACGA GCCGTCCTTA CCAGGCAACC CACTGAAGGA
CGGTCTCGTG ATGGTGGCTT GCGTCTCGGG GAAATGGAAC GTGACTGTTT AATCGGTTAT
GGAGCCAGTA TGCTTTTGCT AGAGAGACTA ATGATTTCAA GTGATGCCTT TGAGGTTGAT
GTCTGTGGGC AGTGTGGACT TCTGGGGTAT TCTGGCTGGT GCCATTACTG CAAGTCATCC
TGCCACGTGT CTTCCCTCCG TATTCCGTAT GCCTGCAAGC TGCTCTTCCA GGAACTACAG
TCTATGAACA TCATCCCCAG GTTAAAACTG TCCAAGTACA ATGAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27279
Clone ID Related Accession (Same CDS sequence) NM_018082
Accession Version NM_018082.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product DNA-directed RNA polymerase III subunit RPC2 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA236776.1, BX537447.1 and BC033724.2. This sequence is a reference standard in the RefSeqGene project. On Jun 5, 2009 this sequence version replaced gi:142370957. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX537447.1, AY092084.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGGACGTGC TAGCGGAGGA GTTTGGGAAC CTGACTCCGG AGCAGCTGGC GGCGCCGATC 
CCGACTGTAG AGGAAAAATG GAGGCTGCTT CCAGCATTTT TAAAGGTGAA AGGCCTTGTG
AAACAGCATA TAGATTCATT TAACTATTTC ATTAATGTAG AGATAAAGAA GATAATGAAA
GCCAATGAAA AGGTTACAAG TGACGCTGAC CCTATGTGGT ACTTAAAATA TCTTAATATC
TATGTTGGGC TTCCTGATGT TGAAGAAAGC TTCAATGTAA CTAGACCAGT GTCCCCTCAT
GAGTGCCGTT TGAGAGACAT GACATACTCT GCCCCTATTA CAGTGGATAT TGAATATACC
CGAGGCAGCC AGAGGATCAT CCGCAATGCC TTACCTATCG GCAGAATGCC CATAATGCTA
CGTAGTTCAA ACTGTGTTCT TACAGGAAAA ACGCCAGCAG AATTTGCCAA ACTGAACGAA
TGTCCCTTAG ATCCAGGTGG CTACTTCATT GTTAAAGGAG TAGAAAAAGT TATTCTTATC
CAAGAGCAGC TGTCTAAGAA CAGGATCATC GTGGAGGCTG ATAGAAAAGG GGCTGTTGGA
GCTTCAGTTA CCAGCTCTAC CCATGAGAAA AAAAGCAGAA CCAATATGGC TGTGAAACAA
GGACGATTTT ATTTGAGGCA TAATACTTTG TCAGAAGATA TACCCATTGT CATCATATTT
AAGGCCATGG GTGTTGAGAG TGACCAGGAA ATTGTGCAGA TGATTGGAAC AGAGGAGCAC
GTGATGGCTG CATTTGGGCC CAGTCTGGAA GAGTGCCAGA AAGCTCAGAT TTTCACACAG
ATGCAGGCAT TAAAATATAT AGGGAACAAA GTAAGAAGGC AAAGGATGTG GGGAGGTGGA
CCAAAGAAAA CCAAAATAGA AGAAGCAAGA GAGCTCCTGG CTTCCACCAT TCTGACCCAT
GTCCCAGTTA AGGAATTCAA TTTCCGAGCC AAATGTATCT ATACTGCAGT GATGGTGCGA
AGAGTTATTC TGGCCCAAGG AGATAATAAA GTTGACGACA GAGATTATTA TGGTAACAAG
CGACTGGAAT TGGCAGGACA GCTTTTATCT CTTCTTTTTG AAGACTTGTT CAAAAAATTT
AATTCTGAAA TGAAAAAGAT TGCCGACCAG GTGATTCCTA AGCAAAGAGC AGCCCAGTTT
GATGTTGTCA AACACATGCG CCAAGACCAG ATCACCAATG GCATGGTGAA TGCTATTTCT
ACCGGAAATT GGTCTTTAAA GAGATTTAAA ATGGACCGCC AGGGTGTAAC CCAAGTGCTG
TCTCGCTTGT CATATATATC CGCACTGGGC ATGATGACAA GAATCTCTTC CCAGTTTGAA
AAAACGAGAA AAGTGAGTGG TCCTCGCTCC CTCCAGCCAT CTCAGTGGGG AATGCTGTGT
CCTTCGGACA CTCCTGAAGG AGAGGCATGT GGTTTGGTTA AAAACTTGGC CCTTATGACA
CACATCACAA CTGATATGGA AGATGGACCC ATTGTTAAAT TAGCCAGTAA CTTGGGAGTA
GAAGATGTGA ATTTATTATG TGGGGAAGAG CTCTCTTACC CAAATGTGTT TCTTGTCTTT
CTTAATGGTA ACATCTTAGG TGTCATTCGA GACCACAAAA AGCTAGTGAA TACATTTCGA
CTCATGAGAA GAGCAGGATA TATCAATGAA TTTGTTTCCA TCTCAACAAA TCTTACAGAT
CGATGTGTCT ATATTTCTTC TGATGGGGGA AGGCTATGCA GACCCTACAT AATTGTCAAG
AAACAGAAGC CAGCAGTCAC AAATAAACAT ATGGAAGAGC TGGCCCAAGG GTACAGGAAT
TTTGAAGATT TCTTACATGA GAGTCTGGTT GAATATTTAG ATGTGAATGA AGAAAATGAT
TGTAACATTG CACTGTACGA ACACACAATT AATAAAGACA CCACCCACTT GGAGATTGAA
CCCTTCACTC TTCTCGGCGT GTGTGCTGGA CTTATCCCAT ACCCTCACCA TAACCAGTCA
CCGAGAAACA CTTATCAGTG TGCCATGGGG AAACAAGCCA TGGGTACTAT AGGATACAAC
CAGCGAAACA GAATTGATAC TCTCATGTAT CTACTAGCAT ATCCACAAAA ACCCATGGTT
AAGACAAAAA CCATTGAATT GATAGAATTT GAGAAACTGC CAGCTGGACA GAATGCAACA
GTTGCTGTGA TGAGCTATAG TGGCTATGAT ATTGAAGATG CTCTTGTTTT AAACAAGGCC
TCTTTAGACA GAGGCTTTGG GCGTTGCCTT GTATATAAAA ATGCTAAATG TACGTTGAAA
CGATACACCA ATCAGACTTT TGATAAAGTG ATGGGGCCCA TGTTGGATGC TGCTACAAGG
AAACCTATCT GGCGACATGA AATCTTAGAT GCAGATGGTA TTTGTTCTCC AGGTGAGAAA
GTAGAAAACA AACAAGTGCT TGTAAATAAG TCCATGCCCA CAGTGACTCA GATTCCTTTG
GAAGGAAGTA ATGTACCACA GCAACCACAG TACAAAGATG TACCCATAAC CTACAAAGGA
GCAACAGACT CATATATTGA AAAAGTGATG ATATCTTCAA ATGCTGAAGA TGCTTTTCTG
ATCAAAATGC TGCTGAGACA GACAAGGCGT CCAGAAATTG GAGACAAATT CAGCAGTCGT
CATGGGCAAA AAGGTGTTTG TGGCTTGATC GTCCCCCAGG AAGACATGCC ATTTTGTGAT
TCTGGCATCT GTCCGGACAT CATCATGAAC CCACACGGCT TCCCATCACG AATGACGGTG
GGGAAGCTCA TTGAGCTGCT GGCTGGCAAG GCCGGTGTGC TGGACGGCAG ATTCCACTAC
GGCACTGCGT TTGGAGGCAG TAAAGTGAAG GATGTGTGTG AGGACCTCGT TCGCCATGGT
TATAACTACT TGGGGAAAGA CTATGTTACA TCCGGCATCA CAGGTGAGCC CTTAGAAGCA
TACATCTATT TTGGCCCCGT GTACTATCAG AAGCTGAAAC ACATGGTGCT AGATAAAATG
CATGCCCGGG CCCGGGGCCC ACGAGCCGTC CTTACCAGGC AACCCACTGA AGGACGGTCT
CGTGATGGTG GCTTGCGTCT CGGGGAAATG GAACGTGACT GTTTAATCGG TTATGGAGCC
AGTATGCTTT TGCTAGAGAG ACTAATGATT TCAAGTGATG CCTTTGAGGT TGATGTCTGT
GGGCAGTGTG GACTTCTGGG GTATTCTGGC TGGTGCCATT ACTGCAAGTC ATCCTGCCAC
GTGTCTTCCC TCCGTATTCC GTATGCCTGC AAGCTGCTCT TCCAGGAACT ACAGTCTATG
AACATCATCC CCAGGTTAAA ACTGTCCAAG TACAATGAAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_060552.4
CDS223..3624
Misc Feature(1)100..102(+)
Misc Feature(2)298..3609(+)
Misc Feature(3)337..3603(+)
Misc Feature(4)535..2682(+)
Misc Feature(5)1621..3603(+)
Misc Feature(6)1837..2022(+)
Misc Feature(7)2083..2205(+)
Misc Feature(8)2368..3234(+)
Misc Feature(9)2467..3201(+)
Misc Feature(10)2509..3225(+)
Exon (1)1..294
Gene:POLR3B
Gene Synonym:
Exon (2)295..327
Gene:POLR3B
Gene Synonym:
Exon (3)328..384
Gene:POLR3B
Gene Synonym:
Exon (4)385..449
Gene:POLR3B
Gene Synonym:
Exon (5)450..525
Gene:POLR3B
Gene Synonym:
Exon (6)526..626
Gene:POLR3B
Gene Synonym:
Exon (7)627..718
Gene:POLR3B
Gene Synonym:
Exon (8)719..836
Gene:POLR3B
Gene Synonym:
Exon (9)837..945
Gene:POLR3B
Gene Synonym:
Exon (10)946..1068
Gene:POLR3B
Gene Synonym:
Exon (11)1069..1188
Gene:POLR3B
Gene Synonym:
Exon (12)1189..1323
Gene:POLR3B
Gene Synonym:
Exon (13)1324..1485
Gene:POLR3B
Gene Synonym:
Exon (14)1486..1686
Gene:POLR3B
Gene Synonym:
Exon (15)1687..1849
Gene:POLR3B
Gene Synonym:
Exon (16)1850..2003
Gene:POLR3B
Gene Synonym:
Exon (17)2004..2078
Gene:POLR3B
Gene Synonym:
Exon (18)2079..2177
Gene:POLR3B
Gene Synonym:
Exon (19)2178..2305
Gene:POLR3B
Gene Synonym:
Exon (20)2306..2515
Gene:POLR3B
Gene Synonym:
Exon (21)2516..2674
Gene:POLR3B
Gene Synonym:
Exon (22)2675..2792
Gene:POLR3B
Gene Synonym:
Exon (23)2793..2935
Gene:POLR3B
Gene Synonym:
Exon (24)2936..3039
Gene:POLR3B
Gene Synonym:
Exon (25)3040..3206
Gene:POLR3B
Gene Synonym:
Exon (26)3207..3320
Gene:POLR3B
Gene Synonym:
Exon (27)3321..3494
Gene:POLR3B
Gene Synonym:
Exon (28)3495..4273
Gene:POLR3B
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_018082
Organism Homo sapiens (human)
Definition Homo sapiens polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_018082

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGGACGTGC TAGCGGAGGA GTTTGGGAAC CTGACTCCGG AGCAGCTGGC GGCGCCGATC 
CCGACTGTAG AGGAAAAATG GAGGCTGCTT CCAGCATTTT TAAAGGTGAA AGGCCTTGTG
AAACAGCATA TAGATTCATT TAACTATTTC ATTAATGTAG AGATAAAGAA GATAATGAAA
GCCAATGAAA AGGTTACAAG TGACGCTGAC CCTATGTGGT ACTTAAAATA TCTTAATATC
TATGTTGGGC TTCCTGATGT TGAAGAAAGC TTCAATGTAA CTAGACCAGT GTCCCCTCAT
GAGTGCCGTT TGAGAGACAT GACATACTCT GCCCCTATTA CAGTGGATAT TGAATATACC
CGAGGCAGCC AGAGGATCAT CCGCAATGCC TTACCTATCG GCAGAATGCC CATAATGCTA
CGTAGTTCAA ACTGTGTTCT TACAGGAAAA ACGCCAGCAG AATTTGCCAA ACTGAACGAA
TGTCCCTTAG ATCCAGGTGG CTACTTCATT GTTAAAGGAG TAGAAAAAGT TATTCTTATC
CAAGAGCAGC TGTCTAAGAA CAGGATCATC GTGGAGGCTG ATAGAAAAGG GGCTGTTGGA
GCTTCAGTTA CCAGCTCTAC CCATGAGAAA AAAAGCAGAA CCAATATGGC TGTGAAACAA
GGACGATTTT ATTTGAGGCA TAATACTTTG TCAGAAGATA TACCCATTGT CATCATATTT
AAGGCCATGG GTGTTGAGAG TGACCAGGAA ATTGTGCAGA TGATTGGAAC AGAGGAGCAC
GTGATGGCTG CATTTGGGCC CAGTCTGGAA GAGTGCCAGA AAGCTCAGAT TTTCACACAG
ATGCAGGCAT TAAAATATAT AGGGAACAAA GTAAGAAGGC AAAGGATGTG GGGAGGTGGA
CCAAAGAAAA CCAAAATAGA AGAAGCAAGA GAGCTCCTGG CTTCCACCAT TCTGACCCAT
GTCCCAGTTA AGGAATTCAA TTTCCGAGCC AAATGTATCT ATACTGCAGT GATGGTGCGA
AGAGTTATTC TGGCCCAAGG AGATAATAAA GTTGACGACA GAGATTATTA TGGTAACAAG
CGACTGGAAT TGGCAGGACA GCTTTTATCT CTTCTTTTTG AAGACTTGTT CAAAAAATTT
AATTCTGAAA TGAAAAAGAT TGCCGACCAG GTGATTCCTA AGCAAAGAGC AGCCCAGTTT
GATGTTGTCA AACACATGCG CCAAGACCAG ATCACCAATG GCATGGTGAA TGCTATTTCT
ACCGGAAATT GGTCTTTAAA GAGATTTAAA ATGGACCGCC AGGGTGTAAC CCAAGTGCTG
TCTCGCTTGT CATATATATC CGCACTGGGC ATGATGACAA GAATCTCTTC CCAGTTTGAA
AAAACGAGAA AAGTGAGTGG TCCTCGCTCC CTCCAGCCAT CTCAGTGGGG AATGCTGTGT
CCTTCGGACA CTCCTGAAGG AGAGGCATGT GGTTTGGTTA AAAACTTGGC CCTTATGACA
CACATCACAA CTGATATGGA AGATGGACCC ATTGTTAAAT TAGCCAGTAA CTTGGGAGTA
GAAGATGTGA ATTTATTATG TGGGGAAGAG CTCTCTTACC CAAATGTGTT TCTTGTCTTT
CTTAATGGTA ACATCTTAGG TGTCATTCGA GACCACAAAA AGCTAGTGAA TACATTTCGA
CTCATGAGAA GAGCAGGATA TATCAATGAA TTTGTTTCCA TCTCAACAAA TCTTACAGAT
CGATGTGTCT ATATTTCTTC TGATGGGGGA AGGCTATGCA GACCCTACAT AATTGTCAAG
AAACAGAAGC CAGCAGTCAC AAATAAACAT ATGGAAGAGC TGGCCCAAGG GTACAGGAAT
TTTGAAGATT TCTTACATGA GAGTCTGGTT GAATATTTAG ATGTGAATGA AGAAAATGAT
TGTAACATTG CACTGTACGA ACACACAATT AATAAAGACA CCACCCACTT GGAGATTGAA
CCCTTCACTC TTCTCGGCGT GTGTGCTGGA CTTATCCCAT ACCCTCACCA TAACCAGTCA
CCGAGAAACA CTTATCAGTG TGCCATGGGG AAACAAGCCA TGGGTACTAT AGGATACAAC
CAGCGAAACA GAATTGATAC TCTCATGTAT CTACTAGCAT ATCCACAAAA ACCCATGGTT
AAGACAAAAA CCATTGAATT GATAGAATTT GAGAAACTGC CAGCTGGACA GAATGCAACA
GTTGCTGTGA TGAGCTATAG TGGCTATGAT ATTGAAGATG CTCTTGTTTT AAACAAGGCC
TCTTTAGACA GAGGCTTTGG GCGTTGCCTT GTATATAAAA ATGCTAAATG TACGTTGAAA
CGATACACCA ATCAGACTTT TGATAAAGTG ATGGGGCCCA TGTTGGATGC TGCTACAAGG
AAACCTATCT GGCGACATGA AATCTTAGAT GCAGATGGTA TTTGTTCTCC AGGTGAGAAA
GTAGAAAACA AACAAGTGCT TGTAAATAAG TCCATGCCCA CAGTGACTCA GATTCCTTTG
GAAGGAAGTA ATGTACCACA GCAACCACAG TACAAAGATG TACCCATAAC CTACAAAGGA
GCAACAGACT CATATATTGA AAAAGTGATG ATATCTTCAA ATGCTGAAGA TGCTTTTCTG
ATCAAAATGC TGCTGAGACA GACAAGGCGT CCAGAAATTG GAGACAAATT CAGCAGTCGT
CATGGGCAAA AAGGTGTTTG TGGCTTGATC GTCCCCCAGG AAGACATGCC ATTTTGTGAT
TCTGGCATCT GTCCGGACAT CATCATGAAC CCACACGGCT TCCCATCACG AATGACGGTG
GGGAAGCTCA TTGAGCTGCT GGCTGGCAAG GCCGGTGTGC TGGACGGCAG ATTCCACTAC
GGCACTGCGT TTGGAGGCAG TAAAGTGAAG GATGTGTGTG AGGACCTCGT TCGCCATGGT
TATAACTACT TGGGGAAAGA CTATGTTACA TCCGGCATCA CAGGTGAGCC CTTAGAAGCA
TACATCTATT TTGGCCCCGT GTACTATCAG AAGCTGAAAC ACATGGTGCT AGATAAAATG
CATGCCCGGG CCCGGGGCCC ACGAGCCGTC CTTACCAGGC AACCCACTGA AGGACGGTCT
CGTGATGGTG GCTTGCGTCT CGGGGAAATG GAACGTGACT GTTTAATCGG TTATGGAGCC
AGTATGCTTT TGCTAGAGAG ACTAATGATT TCAAGTGATG CCTTTGAGGT TGATGTCTGT
GGGCAGTGTG GACTTCTGGG GTATTCTGGC TGGTGCCATT ACTGCAAGTC ATCCTGCCAC
GTGTCTTCCC TCCGTATTCC GTATGCCTGC AAGCTGCTCT TCCAGGAACT ACAGTCTATG
AACATCATCC CCAGGTTAAA ACTGTCCAAG TACAATGAAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Genome-wide association study of Tourette's syndrome
Mol. Psychiatry 18 (6), 721-728 (2013)
Scharf JM, Yu D, Mathews CA, Neale BM, Stewart SE, Fagerness JA, Evans P, Gamazon E, Edlund CK, Service SK, Tikhomirov A, Osiecki L, Illmann C, Pluzhnikov A, Konkashbaev A, Davis LK, Han B, Crane J, Moorjani P, Crenshaw AT, Parkin MA, Reus VI, Lowe TL, Rangel-Lugo M, Chouinard S, Dion Y, Girard S, Cath DC, Smit JH, King RA, Fernandez TV, Leckman JF, Kidd KK, Kidd JR, Pakstis AJ, State MW, Herrera LD, Romero R, Fournier E, Sandor P, Barr CL, Phan N, Gross-Tsur V, Benarroch F, Pollak Y, Budman CL, Bruun RD, Erenberg G, Naarden AL, Lee PC, Weiss N, Kremeyer B, Berrio GB, Campbell DD, Cardona Silgado JC, Ochoa WC, Mesa Restrepo SC, Muller H, Valencia Duarte AV, Lyon GJ, Leppert M, Morgan J, Weiss R, Grados MA, Anderson K, Davarya S, Singer H, Walkup J, Jankovic J, Tischfield JA, Heiman GA, Gilbert DL, Hoekstra PJ, Robertson MM, Kurlan R, Liu C, Gibbs JR, Singleton A, Hardy J, Strengman E, Ophoff RA, Wagner M, Moessner R, Mirel DB, Posthuma D, Sabatti C, Eskin E, Conti DV, Knowles JA, Ruiz-Linares A, Rouleau GA, Purcell S, Heutink P, Oostra BA, McMahon WM, Freimer NB, Cox NJ and Pauls DL.


book

Mutations in POLR3A and POLR3B are a major cause of hypomyelinating leukodystrophies with or without dental abnormalities and/or hypogonadotropic hypogonadism
J. Med. Genet. 50 (3), 194-197 (2013)
Daoud H, Tetreault M, Gibson W, Guerrero K, Cohen A, Gburek-Augustat J, Synofzik M, Brais B, Stevens CA, Sanchez-Carpintero R, Goizet C, Naidu S, Vanderver A and Bernard G.


book

INMAP, a novel truncated version of POLR3B, represses AP-1 and p53 transcriptional activity
Mol. Cell. Biochem. 374 (1-2), 81-89 (2013)
Yunlei Z, Zhe C, Yan L, Pengcheng W, Yanbo Z, Le S and Qianjin L.


book

Recessive mutations in POLR3B, encoding the second largest subunit of Pol III, cause a rare hypomyelinating leukodystrophy
Am. J. Hum. Genet. 89 (5), 652-655 (2011)
Tetreault M, Choquet K, Orcesi S, Tonduti D, Balottin U, Teichmann M, Fribourg S, Schiffmann R, Brais B, Vanderver A and Bernard G.


book

Mutations in POLR3A and POLR3B encoding RNA Polymerase III subunits cause an autosomal-recessive hypomyelinating leukoencephalopathy
Am. J. Hum. Genet. 89 (5), 644-651 (2011)
Saitsu H, Osaka H, Sasaki M, Takanashi J, Hamada K, Yamashita A, Shibayama H, Shiina M, Kondo Y, Nishiyama K, Tsurusaki Y, Miyake N, Doi H, Ogata K, Inoue K and Matsumoto N.


book

RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate
Nat. Immunol. 10 (10), 1065-1072 (2009)
Ablasser A, Bauernfeind F, Hartmann G, Latz E, Fitzgerald KA and Hornung V.


book

Identification and characterization of INMAP, a novel interphase nucleus and mitotic apparatus protein that is involved in spindle formation and cell cycle progression
Exp. Cell Res. 315 (7), 1100-1116 (2009)
Shen E, Lei Y, Liu Q, Zheng Y, Song C, Marc J, Wang Y, Sun L and Liang Q.


book

Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits
Mol. Cell. Biol. 22 (22), 8044-8055 (2002)
Hu P, Wu S, Sun Y, Yuan CC, Kobayashi R, Myers MP and Hernandez N.


book

Leukodystrophy Overview
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Vanderver,A., Tonduti,D., Schiffmann,R., Schmidt,J. and Van der Knaap,M.S.


book

Pol III-Related Leukodystrophies
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Bernard,G. and Vanderver,A.


 
*
*
*
*