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VPS35 cDNA ORF clone, Homo sapiens (human)

Gene Symbol VPS35
Entrez Gene ID 55737
Full Name VPS35 retromer complex component
Synonyms MEM3, PARK17
General protein information
Preferred Names
vacuolar protein sorting-associated protein 35
Names
vacuolar protein sorting-associated protein 35
hVPS35
maternal-embryonic 3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

16

16q12

Summary This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

601501


mRNA and Protein(s)

mRNA Protein Name
XM_011523227 XP_011521529 vacuolar protein sorting-associated protein 35 isoform X1
XM_005256045 XP_005256102 vacuolar protein sorting-associated protein 35 isoform X2
NM_018206 NP_060676 vacuolar protein sorting-associated protein 35



Homo sapiens (human) VPS35 NP_060676.2
Pan troglodytes (chimpanzee) VPS35 XP_001161536.1
Macaca mulatta (Rhesus monkey) VPS35 XP_001108828.2
Canis lupus familiaris (dog) VPS35 XP_532570.3
Bos taurus (cattle) VPS35 NP_001039723.1
Mus musculus (house mouse) Vps35 NP_075373.1
Rattus norvegicus (Norway rat) Vps35 NP_001099188.2
Gallus gallus (chicken) VPS35 NP_001005842.1
Danio rerio (zebrafish) vps35 NP_001020688.2
Drosophila melanogaster (fruit fly) Vps35 NP_611651.4
Caenorhabditis elegans vps-35 NP_495180.2
Arabidopsis thaliana (thale cress) VPS35B NP_177713.3


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following VPS35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu69690 XM_011523227 PREDICTED: Homo sapiens vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu49428 XM_005256045 PREDICTED: Homo sapiens vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu18510
NM_018206 Homo sapiens VPS35 retromer complex component (VPS35), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu69690
Clone ID Related Accession (Same CDS sequence) XM_011523227
Accession Version XM_011523227.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product vacuolar protein sorting-associated protein 35 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
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181
241
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361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGAAGAGAT GCCTGGACAA AAACAAGCTT ATGGATGCTC TAAAACATGC TTCTAATATG 
CTTGGTGAAC TCCGGACTTC TATGTTATCA CCAAAGAGTT ACTATGAACT TTATATGGCC
ATTTCTGATG AACTGCACTA CTTGGAGGTC TACCTGACAG ATGAGTTTGC TAAAGGAAGG
AAAGTGGCAG ATCTCTACGA ACTTGTACAG TATGCTGGAA ACATTATCCC AAGGCTTTAC
CTTTTGATCA CAGTTGGAGT TGTATATGTC AAGTCATTTC CTCAGTCCAG GAAGGATATT
TTGAAAGATT TGGTAGAAAT GTGCCGTGGT GTGCAACATC CCTTGAGGGG TCTGTTTCTT
CGAAATTACC TTCTTCAGTG TACCAGAAAT ATCTTACCTG ATGAAGGAGA GCCAACAGAT
GAAGAAACAA CTGGTGACAT CAGTGATTCC ATGGATTTTG TACTGCTCAA CTTTGCAGAA
ATGAACAAGC TCTGGGTGCG AATGCAGCAT CAGGGACATA GCCGAGATAG AGAAAAAAGA
GAACGAGAAA GACAAGAACT GAGAATTTTA GTGGGAACAA ATTTGGTGCG CCTCAGTCAG
TTGGAAGGTG TAAATGTGGA ACGTTACAAA CAGATTGTTT TGACTGGCAT ATTGGAGCAA
GTTGTAAACT GTAGGGATGC TTTGGCTCAA GAATATCTCA TGGAGTGTAT TATTCAGGTT
TTCCCTGATG AATTTCACCT CCAGACTTTG AATCCTTTTC TTCGGGCCTG TGCTGAGTTA
CACCAGAATG TAAATGTGAA GAACATAATC ATTGCTTTAA TTGATAGATT AGCTTTATTT
GCTCACCGTG AAGATGGACC TGGAATCCCA GCGGATATTA AACTTTTTGA TATATTTTCA
CAGCAGGTGG CTACAGTGAT ACAGTCTAGA CAAGACATGC CTTCAGAGGA TGTTGTATCT
TTACAAGTCT CTCTGATTAA TCTTGCCATG AAATGTTACC CTGATCGTGT GGACTATGTT
GATAAAGTTC TAGAAACAAC AGTGGAGATA TTCAATAAGC TCAACCTTGA ACATATTGCT
ACCAGTAGTG CAGTTTCAAA GGAACTCACC AGACTTTTGA AAATACCAGT TGACACTTAC
AACAATATTT TAACAGTCTT GAAATTAAAA CATTTTCACC CACTCTTTGA GTACTTTGAC
TACGAGTCCA GAAAGAGCAT GAGTTGTTAT GTGCTTAGTA ATGTTCTGGA TTATAACACA
GAAATTGTCT CTCAAGACCA GGTGGATTCC ATAATGAATT TGGTATCCAC GTTGATTCAA
GATCAGCCAG ATCAACCTGT AGAAGACCCT GATCCAGAAG ATTTTGCTGA TGAGCAGAGC
CTTGTGGGCC GCTTCATTCA TCTGCTGCGC TCTGAGGACC CTGACCAGCA GTACTTGATT
TTGAACACAG CACGAAAACA TTTTGGAGCT GGTGGAAATC AGCGGATTCG CTTCACACTG
CCACCTTTGG TATTTGCAGC TTACCAGCTG GCTTTTCGAT ATAAAGAGAA TTCTAAAGTG
GATGACAAAT GGGAAAAGAA ATGCCAGAAG ATTTTTTCAT TTGCCCACCA GACTATCAGT
GCTTTGATCA AAGCAGAGCT GGCAGAATTG CCCTTAAGAC TTTTTCTTCA AGGAGCACTA
GCTGCTGGGG AAATTGGTTT TGAAAATCAT GAGACAGTCG CATATGAATT CATGTCCCAG
GCATTTTCTC TGTATGAAGA TGAAATCAGC GATTCCAAAG CACAGCTAGC TGCCATCACC
TTGATCATTG GCACTTTTGA AAGGATGAAG TGCTTCAGTG AAGAGAATCA CGAACCTCTG
AGGACTCAGT GTGCCCTTGC TGCATCCAAA CTTCTAAAGA AACCTGATCA GGGCCGAGCT
GTGAGCACCT GTGCACATCT CTTCTGGTCT GGCAGAAACA CGGACAAAAA TGGGGAGGAG
CTTCACGGAG GCAAGAGGGT AATGGAGTGC CTAAAAAAAG CTCTAAAAAT AGCAAATCAG
TGCATGGACC CCTCTCTACA AGTGCAGCTT TTTATAGAAA TTCTGAACAG ATATATCTAT
TTTTATGAAA AGGAAAATGA TGCGGTAACA ATTCAGGTTT TAAACCAGCT TATCCAAAAG
ATTCGAGAAG ACCTCCCGAA TCTTGAATCC AGTGAAGAAA CAGAGCAGAT TAACAAACAT
TTTCATAACA CACTGGAGCA TTTGCGCTTG CGGCGGGAAT CACCAGAATC CGAGGGGCCA
ATTTATGAAG GTCTCATCCT TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011521529.1
CDS880..3183
Misc Feature(1)880..3054(+)
Translation

Target ORF information:

RefSeq Version XM_011523227
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011523227

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGAAGAGAT GCCTGGACAA AAACAAGCTT ATGGATGCTC TAAAACATGC TTCTAATATG 
CTTGGTGAAC TCCGGACTTC TATGTTATCA CCAAAGAGTT ACTATGAACT TTATATGGCC
ATTTCTGATG AACTGCACTA CTTGGAGGTC TACCTGACAG ATGAGTTTGC TAAAGGAAGG
AAAGTGGCAG ATCTCTACGA ACTTGTACAG TATGCTGGAA ACATTATCCC AAGGCTTTAC
CTTTTGATCA CAGTTGGAGT TGTATATGTC AAGTCATTTC CTCAGTCCAG GAAGGATATT
TTGAAAGATT TGGTAGAAAT GTGCCGTGGT GTGCAACATC CCTTGAGGGG TCTGTTTCTT
CGAAATTACC TTCTTCAGTG TACCAGAAAT ATCTTACCTG ATGAAGGAGA GCCAACAGAT
GAAGAAACAA CTGGTGACAT CAGTGATTCC ATGGATTTTG TACTGCTCAA CTTTGCAGAA
ATGAACAAGC TCTGGGTGCG AATGCAGCAT CAGGGACATA GCCGAGATAG AGAAAAAAGA
GAACGAGAAA GACAAGAACT GAGAATTTTA GTGGGAACAA ATTTGGTGCG CCTCAGTCAG
TTGGAAGGTG TAAATGTGGA ACGTTACAAA CAGATTGTTT TGACTGGCAT ATTGGAGCAA
GTTGTAAACT GTAGGGATGC TTTGGCTCAA GAATATCTCA TGGAGTGTAT TATTCAGGTT
TTCCCTGATG AATTTCACCT CCAGACTTTG AATCCTTTTC TTCGGGCCTG TGCTGAGTTA
CACCAGAATG TAAATGTGAA GAACATAATC ATTGCTTTAA TTGATAGATT AGCTTTATTT
GCTCACCGTG AAGATGGACC TGGAATCCCA GCGGATATTA AACTTTTTGA TATATTTTCA
CAGCAGGTGG CTACAGTGAT ACAGTCTAGA CAAGACATGC CTTCAGAGGA TGTTGTATCT
TTACAAGTCT CTCTGATTAA TCTTGCCATG AAATGTTACC CTGATCGTGT GGACTATGTT
GATAAAGTTC TAGAAACAAC AGTGGAGATA TTCAATAAGC TCAACCTTGA ACATATTGCT
ACCAGTAGTG CAGTTTCAAA GGAACTCACC AGACTTTTGA AAATACCAGT TGACACTTAC
AACAATATTT TAACAGTCTT GAAATTAAAA CATTTTCACC CACTCTTTGA GTACTTTGAC
TACGAGTCCA GAAAGAGCAT GAGTTGTTAT GTGCTTAGTA ATGTTCTGGA TTATAACACA
GAAATTGTCT CTCAAGACCA GGTGGATTCC ATAATGAATT TGGTATCCAC GTTGATTCAA
GATCAGCCAG ATCAACCTGT AGAAGACCCT GATCCAGAAG ATTTTGCTGA TGAGCAGAGC
CTTGTGGGCC GCTTCATTCA TCTGCTGCGC TCTGAGGACC CTGACCAGCA GTACTTGATT
TTGAACACAG CACGAAAACA TTTTGGAGCT GGTGGAAATC AGCGGATTCG CTTCACACTG
CCACCTTTGG TATTTGCAGC TTACCAGCTG GCTTTTCGAT ATAAAGAGAA TTCTAAAGTG
GATGACAAAT GGGAAAAGAA ATGCCAGAAG ATTTTTTCAT TTGCCCACCA GACTATCAGT
GCTTTGATCA AAGCAGAGCT GGCAGAATTG CCCTTAAGAC TTTTTCTTCA AGGAGCACTA
GCTGCTGGGG AAATTGGTTT TGAAAATCAT GAGACAGTCG CATATGAATT CATGTCCCAG
GCATTTTCTC TGTATGAAGA TGAAATCAGC GATTCCAAAG CACAGCTAGC TGCCATCACC
TTGATCATTG GCACTTTTGA AAGGATGAAG TGCTTCAGTG AAGAGAATCA CGAACCTCTG
AGGACTCAGT GTGCCCTTGC TGCATCCAAA CTTCTAAAGA AACCTGATCA GGGCCGAGCT
GTGAGCACCT GTGCACATCT CTTCTGGTCT GGCAGAAACA CGGACAAAAA TGGGGAGGAG
CTTCACGGAG GCAAGAGGGT AATGGAGTGC CTAAAAAAAG CTCTAAAAAT AGCAAATCAG
TGCATGGACC CCTCTCTACA AGTGCAGCTT TTTATAGAAA TTCTGAACAG ATATATCTAT
TTTTATGAAA AGGAAAATGA TGCGGTAACA ATTCAGGTTT TAAACCAGCT TATCCAAAAG
ATTCGAGAAG ACCTCCCGAA TCTTGAATCC AGTGAAGAAA CAGAGCAGAT TAACAAACAT
TTTCATAACA CACTGGAGCA TTTGCGCTTG CGGCGGGAAT CACCAGAATC CGAGGGGCCA
ATTTATGAAG GTCTCATCCT TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu49428
Clone ID Related Accession (Same CDS sequence) XM_005256045
Accession Version XM_005256045.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product vacuolar protein sorting-associated protein 35 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530424104. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCCATTT CTGATGAACT GCACTACTTG GAGGTCTACC TGACAGATGA GTTTGCTAAA 
GGAAGGAAAG TGGCAGATCT CTACGAACTT GTACAGTATG CTGGAAACAT TATCCCAAGG
CTTTACCTTT TGATCACAGT TGGAGTTGTA TATGTCAAGT CATTTCCTCA GTCCAGGAAG
GATATTTTGA AAGATTTGGT AGAAATGTGC CGTGGTGTGC AACATCCCTT GAGGGGTCTG
TTTCTTCGAA ATTACCTTCT TCAGTGTACC AGAAATATCT TACCTGATGA AGGAGAGCCA
ACAGATGAAG AAACAACTGG TGACATCAGT GATTCCATGG ATTTTGTACT GCTCAACTTT
GCAGAAATGA ACAAGCTCTG GGTGCGAATG CAGCATCAGG GACATAGCCG AGATAGAGAA
AAAAGAGAAC GAGAAAGACA AGAACTGAGA ATTTTAGTGG GAACAAATTT GGTGCGCCTC
AGTCAGTTGG AAGGTGTAAA TGTGGAACGT TACAAACAGA TTGTTTTGAC TGGCATATTG
GAGCAAGTTG TAAACTGTAG GGATGCTTTG GCTCAAGAAT ATCTCATGGA GTGTATTATT
CAGGTTTTCC CTGATGAATT TCACCTCCAG ACTTTGAATC CTTTTCTTCG GGCCTGTGCT
GAGTTACACC AGAATGTAAA TGTGAAGAAC ATAATCATTG CTTTAATTGA TAGATTAGCT
TTATTTGCTC ACCGTGAAGA TGGACCTGGA ATCCCAGCGG ATATTAAACT TTTTGATATA
TTTTCACAGC AGGTGGCTAC AGTGATACAG TCTAGACAAG ACATGCCTTC AGAGGATGTT
GTATCTTTAC AAGTCTCTCT GATTAATCTT GCCATGAAAT GTTACCCTGA TCGTGTGGAC
TATGTTGATA AAGTTCTAGA AACAACAGTG GAGATATTCA ATAAGCTCAA CCTTGAACAT
ATTGCTACCA GTAGTGCAGT TTCAAAGGAA CTCACCAGAC TTTTGAAAAT ACCAGTTGAC
ACTTACAACA ATATTTTAAC AGTCTTGAAA TTAAAACATT TTCACCCACT CTTTGAGTAC
TTTGACTACG AGTCCAGAAA GAGCATGAGT TGTTATGTGC TTAGTAATGT TCTGGATTAT
AACACAGAAA TTGTCTCTCA AGACCAGGTG GATTCCATAA TGAATTTGGT ATCCACGTTG
ATTCAAGATC AGCCAGATCA ACCTGTAGAA GACCCTGATC CAGAAGATTT TGCTGATGAG
CAGAGCCTTG TGGGCCGCTT CATTCATCTG CTGCGCTCTG AGGACCCTGA CCAGCAGTAC
TTGATTTTGA ACACAGCACG AAAACATTTT GGAGCTGGTG GAAATCAGCG GATTCGCTTC
ACACTGCCAC CTTTGGTATT TGCAGCTTAC CAGCTGGCTT TTCGATATAA AGAGAATTCT
AAAGTGGATG ACAAATGGGA AAAGAAATGC CAGAAGATTT TTTCATTTGC CCACCAGACT
ATCAGTGCTT TGATCAAAGC AGAGCTGGCA GAATTGCCCT TAAGACTTTT TCTTCAAGGA
GCACTAGCTG CTGGGGAAAT TGGTTTTGAA AATCATGAGA CAGTCGCATA TGAATTCATG
TCCCAGGCAT TTTCTCTGTA TGAAGATGAA ATCAGCGATT CCAAAGCACA GCTAGCTGCC
ATCACCTTGA TCATTGGCAC TTTTGAAAGG ATGAAGTGCT TCAGTGAAGA GAATCACGAA
CCTCTGAGGA CTCAGTGTGC CCTTGCTGCA TCCAAACTTC TAAAGAAACC TGATCAGGGC
CGAGCTGTGA GCACCTGTGC ACATCTCTTC TGGTCTGGCA GAAACACGGA CAAAAATGGG
GAGGAGCTTC ACGGAGGCAA GAGGGTAATG GAGTGCCTAA AAAAAGCTCT AAAAATAGCA
AATCAGTGCA TGGACCCCTC TCTACAAGTG CAGCTTTTTA TAGAAATTCT GAACAGATAT
ATCTATTTTT ATGAAAAGGA AAATGATGCG GTAACAATTC AGGTTTTAAA CCAGCTTATC
CAAAAGATTC GAGAAGACCT CCCGAATCTT GAATCCAGTG AAGAAACAGA GCAGATTAAC
AAACATTTTC ATAACACACT GGAGCATTTG CGCTTGCGGC GGGAATCACC AGAATCCGAG
GGGCCAATTT ATGAAGGTCT CATCCTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005256102.1
CDS186..2375
Misc Feature(1)186..2246(+)
Translation

Target ORF information:

RefSeq Version XM_005256045
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005256045

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCCATTT CTGATGAACT GCACTACTTG GAGGTCTACC TGACAGATGA GTTTGCTAAA 
GGAAGGAAAG TGGCAGATCT CTACGAACTT GTACAGTATG CTGGAAACAT TATCCCAAGG
CTTTACCTTT TGATCACAGT TGGAGTTGTA TATGTCAAGT CATTTCCTCA GTCCAGGAAG
GATATTTTGA AAGATTTGGT AGAAATGTGC CGTGGTGTGC AACATCCCTT GAGGGGTCTG
TTTCTTCGAA ATTACCTTCT TCAGTGTACC AGAAATATCT TACCTGATGA AGGAGAGCCA
ACAGATGAAG AAACAACTGG TGACATCAGT GATTCCATGG ATTTTGTACT GCTCAACTTT
GCAGAAATGA ACAAGCTCTG GGTGCGAATG CAGCATCAGG GACATAGCCG AGATAGAGAA
AAAAGAGAAC GAGAAAGACA AGAACTGAGA ATTTTAGTGG GAACAAATTT GGTGCGCCTC
AGTCAGTTGG AAGGTGTAAA TGTGGAACGT TACAAACAGA TTGTTTTGAC TGGCATATTG
GAGCAAGTTG TAAACTGTAG GGATGCTTTG GCTCAAGAAT ATCTCATGGA GTGTATTATT
CAGGTTTTCC CTGATGAATT TCACCTCCAG ACTTTGAATC CTTTTCTTCG GGCCTGTGCT
GAGTTACACC AGAATGTAAA TGTGAAGAAC ATAATCATTG CTTTAATTGA TAGATTAGCT
TTATTTGCTC ACCGTGAAGA TGGACCTGGA ATCCCAGCGG ATATTAAACT TTTTGATATA
TTTTCACAGC AGGTGGCTAC AGTGATACAG TCTAGACAAG ACATGCCTTC AGAGGATGTT
GTATCTTTAC AAGTCTCTCT GATTAATCTT GCCATGAAAT GTTACCCTGA TCGTGTGGAC
TATGTTGATA AAGTTCTAGA AACAACAGTG GAGATATTCA ATAAGCTCAA CCTTGAACAT
ATTGCTACCA GTAGTGCAGT TTCAAAGGAA CTCACCAGAC TTTTGAAAAT ACCAGTTGAC
ACTTACAACA ATATTTTAAC AGTCTTGAAA TTAAAACATT TTCACCCACT CTTTGAGTAC
TTTGACTACG AGTCCAGAAA GAGCATGAGT TGTTATGTGC TTAGTAATGT TCTGGATTAT
AACACAGAAA TTGTCTCTCA AGACCAGGTG GATTCCATAA TGAATTTGGT ATCCACGTTG
ATTCAAGATC AGCCAGATCA ACCTGTAGAA GACCCTGATC CAGAAGATTT TGCTGATGAG
CAGAGCCTTG TGGGCCGCTT CATTCATCTG CTGCGCTCTG AGGACCCTGA CCAGCAGTAC
TTGATTTTGA ACACAGCACG AAAACATTTT GGAGCTGGTG GAAATCAGCG GATTCGCTTC
ACACTGCCAC CTTTGGTATT TGCAGCTTAC CAGCTGGCTT TTCGATATAA AGAGAATTCT
AAAGTGGATG ACAAATGGGA AAAGAAATGC CAGAAGATTT TTTCATTTGC CCACCAGACT
ATCAGTGCTT TGATCAAAGC AGAGCTGGCA GAATTGCCCT TAAGACTTTT TCTTCAAGGA
GCACTAGCTG CTGGGGAAAT TGGTTTTGAA AATCATGAGA CAGTCGCATA TGAATTCATG
TCCCAGGCAT TTTCTCTGTA TGAAGATGAA ATCAGCGATT CCAAAGCACA GCTAGCTGCC
ATCACCTTGA TCATTGGCAC TTTTGAAAGG ATGAAGTGCT TCAGTGAAGA GAATCACGAA
CCTCTGAGGA CTCAGTGTGC CCTTGCTGCA TCCAAACTTC TAAAGAAACC TGATCAGGGC
CGAGCTGTGA GCACCTGTGC ACATCTCTTC TGGTCTGGCA GAAACACGGA CAAAAATGGG
GAGGAGCTTC ACGGAGGCAA GAGGGTAATG GAGTGCCTAA AAAAAGCTCT AAAAATAGCA
AATCAGTGCA TGGACCCCTC TCTACAAGTG CAGCTTTTTA TAGAAATTCT GAACAGATAT
ATCTATTTTT ATGAAAAGGA AAATGATGCG GTAACAATTC AGGTTTTAAA CCAGCTTATC
CAAAAGATTC GAGAAGACCT CCCGAATCTT GAATCCAGTG AAGAAACAGA GCAGATTAAC
AAACATTTTC ATAACACACT GGAGCATTTG CGCTTGCGGC GGGAATCACC AGAATCCGAG
GGGCCAATTT ATGAAGGTCT CATCCTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18510
Clone ID Related Accession (Same CDS sequence) NM_018206
Accession Version NM_018206.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Homo sapiens (human)
Product vacuolar protein sorting-associated protein 35
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB049686.1, BC093036.1, BQ574179.1 and BM971213.1. This sequence is a reference standard in the RefSeqGene project. On Aug 29, 2007 this sequence version replaced gi:41352714. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC093036.1, AF175265.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGCCTACAA CACAGCAGTC CCCTCAGGAT GAGCAGGAAA AGCTCTTGGA TGAAGCCATA 
CAGGCTGTGA AGGTCCAGTC ATTCCAAATG AAGAGATGCC TGGACAAAAA CAAGCTTATG
GATGCTCTAA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA
AAGAGTTACT ATGAACTTTA TATGGCCATT TCTGATGAAC TGCACTACTT GGAGGTCTAC
CTGACAGATG AGTTTGCTAA AGGAAGGAAA GTGGCAGATC TCTACGAACT TGTACAGTAT
GCTGGAAACA TTATCCCAAG GCTTTACCTT TTGATCACAG TTGGAGTTGT ATATGTCAAG
TCATTTCCTC AGTCCAGGAA GGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG
CAACATCCCT TGAGGGGTCT GTTTCTTCGA AATTACCTTC TTCAGTGTAC CAGAAATATC
TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGACATCAG TGATTCCATG
GATTTTGTAC TGCTCAACTT TGCAGAAATG AACAAGCTCT GGGTGCGAAT GCAGCATCAG
GGACATAGCC GAGATAGAGA AAAAAGAGAA CGAGAAAGAC AAGAACTGAG AATTTTAGTG
GGAACAAATT TGGTGCGCCT CAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG
ATTGTTTTGA CTGGCATATT GGAGCAAGTT GTAAACTGTA GGGATGCTTT GGCTCAAGAA
TATCTCATGG AGTGTATTAT TCAGGTTTTC CCTGATGAAT TTCACCTCCA GACTTTGAAT
CCTTTTCTTC GGGCCTGTGC TGAGTTACAC CAGAATGTAA ATGTGAAGAA CATAATCATT
GCTTTAATTG ATAGATTAGC TTTATTTGCT CACCGTGAAG ATGGACCTGG AATCCCAGCG
GATATTAAAC TTTTTGATAT ATTTTCACAG CAGGTGGCTA CAGTGATACA GTCTAGACAA
GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TGATTAATCT TGCCATGAAA
TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC
AATAAGCTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA
CTTTTGAAAA TACCAGTTGA CACTTACAAC AATATTTTAA CAGTCTTGAA ATTAAAACAT
TTTCACCCAC TCTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG TTGTTATGTG
CTTAGTAATG TTCTGGATTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA
ATGAATTTGG TATCCACGTT GATTCAAGAT CAGCCAGATC AACCTGTAGA AGACCCTGAT
CCAGAAGATT TTGCTGATGA GCAGAGCCTT GTGGGCCGCT TCATTCATCT GCTGCGCTCT
GAGGACCCTG ACCAGCAGTA CTTGATTTTG AACACAGCAC GAAAACATTT TGGAGCTGGT
GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT
TTTCGATATA AAGAGAATTC TAAAGTGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT
TTTTCATTTG CCCACCAGAC TATCAGTGCT TTGATCAAAG CAGAGCTGGC AGAATTGCCC
TTAAGACTTT TTCTTCAAGG AGCACTAGCT GCTGGGGAAA TTGGTTTTGA AAATCATGAG
ACAGTCGCAT ATGAATTCAT GTCCCAGGCA TTTTCTCTGT ATGAAGATGA AATCAGCGAT
TCCAAAGCAC AGCTAGCTGC CATCACCTTG ATCATTGGCA CTTTTGAAAG GATGAAGTGC
TTCAGTGAAG AGAATCATGA ACCTCTGAGG ACTCAGTGTG CCCTTGCTGC ATCCAAACTT
CTAAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CACATCTCTT CTGGTCTGGC
AGAAACACGG ACAAAAATGG GGAGGAGCTT CACGGAGGCA AGAGGGTAAT GGAGTGCCTA
AAAAAAGCTC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT GCAGCTTTTT
ATAGAAATTC TGAACAGATA TATCTATTTT TATGAAAAGG AAAATGATGC GGTAACAATT
CAGGTTTTAA ACCAGCTTAT CCAAAAGATT CGAGAAGACC TCCCGAATCT TGAATCCAGT
GAAGAAACAG AGCAGATTAA CAAACATTTT CATAACACAC TGGAGCATTT GCGCTTGCGG
CGGGAATCAC CAGAATCCGA GGGGCCAATT TATGAAGGTC TCATCCTTTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_060676.2
CDS100..2490
Misc Feature(1)40..42(+)
Misc Feature(2)118..120(+)
Misc Feature(3)139..2361(+)
Misc Feature(4)172..231(+)
Misc Feature(5)712..744(+)
Misc Feature(6)1411..2487(+)
Misc Feature(7)1597..2178(+)
Misc Feature(8)2470..2472(+)
Exon (1)1..102
Gene:VPS35
Gene Synonym:
Exon (2)103..201
Gene:VPS35
Gene Synonym:
Exon (3)202..298
Gene:VPS35
Gene Synonym:
Exon (4)299..422
Gene:VPS35
Gene Synonym:
Exon (5)423..605
Gene:VPS35
Gene Synonym:
Exon (6)606..819
Gene:VPS35
Gene Synonym:
Exon (7)820..903
Gene:VPS35
Gene Synonym:
Exon (8)904..1013
Gene:VPS35
Gene Synonym:
Exon (9)1014..1110
Gene:VPS35
Gene Synonym:
Exon (10)1111..1259
Gene:VPS35
Gene Synonym:
Exon (11)1260..1467
Gene:VPS35
Gene Synonym:
Exon (12)1468..1623
Gene:VPS35
Gene Synonym:
Exon (13)1624..1746
Gene:VPS35
Gene Synonym:
Exon (14)1747..1926
Gene:VPS35
Gene Synonym:
Exon (15)1927..2166
Gene:VPS35
Gene Synonym:
Exon (16)2167..2310
Gene:VPS35
Gene Synonym:
Exon (17)2311..3285
Gene:VPS35
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_018206
Organism Homo sapiens (human)
Definition Homo sapiens VPS35 retromer complex component (VPS35), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_018206

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGCCTACAA CACAGCAGTC CCCTCAGGAT GAGCAGGAAA AGCTCTTGGA TGAAGCCATA 
CAGGCTGTGA AGGTCCAGTC ATTCCAAATG AAGAGATGCC TGGACAAAAA CAAGCTTATG
GATGCTCTAA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA
AAGAGTTACT ATGAACTTTA TATGGCCATT TCTGATGAAC TGCACTACTT GGAGGTCTAC
CTGACAGATG AGTTTGCTAA AGGAAGGAAA GTGGCAGATC TCTACGAACT TGTACAGTAT
GCTGGAAACA TTATCCCAAG GCTTTACCTT TTGATCACAG TTGGAGTTGT ATATGTCAAG
TCATTTCCTC AGTCCAGGAA GGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG
CAACATCCCT TGAGGGGTCT GTTTCTTCGA AATTACCTTC TTCAGTGTAC CAGAAATATC
TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGACATCAG TGATTCCATG
GATTTTGTAC TGCTCAACTT TGCAGAAATG AACAAGCTCT GGGTGCGAAT GCAGCATCAG
GGACATAGCC GAGATAGAGA AAAAAGAGAA CGAGAAAGAC AAGAACTGAG AATTTTAGTG
GGAACAAATT TGGTGCGCCT CAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG
ATTGTTTTGA CTGGCATATT GGAGCAAGTT GTAAACTGTA GGGATGCTTT GGCTCAAGAA
TATCTCATGG AGTGTATTAT TCAGGTTTTC CCTGATGAAT TTCACCTCCA GACTTTGAAT
CCTTTTCTTC GGGCCTGTGC TGAGTTACAC CAGAATGTAA ATGTGAAGAA CATAATCATT
GCTTTAATTG ATAGATTAGC TTTATTTGCT CACCGTGAAG ATGGACCTGG AATCCCAGCG
GATATTAAAC TTTTTGATAT ATTTTCACAG CAGGTGGCTA CAGTGATACA GTCTAGACAA
GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TGATTAATCT TGCCATGAAA
TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC
AATAAGCTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA
CTTTTGAAAA TACCAGTTGA CACTTACAAC AATATTTTAA CAGTCTTGAA ATTAAAACAT
TTTCACCCAC TCTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG TTGTTATGTG
CTTAGTAATG TTCTGGATTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA
ATGAATTTGG TATCCACGTT GATTCAAGAT CAGCCAGATC AACCTGTAGA AGACCCTGAT
CCAGAAGATT TTGCTGATGA GCAGAGCCTT GTGGGCCGCT TCATTCATCT GCTGCGCTCT
GAGGACCCTG ACCAGCAGTA CTTGATTTTG AACACAGCAC GAAAACATTT TGGAGCTGGT
GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT
TTTCGATATA AAGAGAATTC TAAAGTGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT
TTTTCATTTG CCCACCAGAC TATCAGTGCT TTGATCAAAG CAGAGCTGGC AGAATTGCCC
TTAAGACTTT TTCTTCAAGG AGCACTAGCT GCTGGGGAAA TTGGTTTTGA AAATCATGAG
ACAGTCGCAT ATGAATTCAT GTCCCAGGCA TTTTCTCTGT ATGAAGATGA AATCAGCGAT
TCCAAAGCAC AGCTAGCTGC CATCACCTTG ATCATTGGCA CTTTTGAAAG GATGAAGTGC
TTCAGTGAAG AGAATCATGA ACCTCTGAGG ACTCAGTGTG CCCTTGCTGC ATCCAAACTT
CTAAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CACATCTCTT CTGGTCTGGC
AGAAACACGG ACAAAAATGG GGAGGAGCTT CACGGAGGCA AGAGGGTAAT GGAGTGCCTA
AAAAAAGCTC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT GCAGCTTTTT
ATAGAAATTC TGAACAGATA TATCTATTTT TATGAAAAGG AAAATGATGC GGTAACAATT
CAGGTTTTAA ACCAGCTTAT CCAAAAGATT CGAGAAGACC TCCCGAATCT TGAATCCAGT
GAAGAAACAG AGCAGATTAA CAAACATTTT CATAACACAC TGGAGCATTT GCGCTTGCGG
CGGGAATCAC CAGAATCCGA GGGGCCAATT TATGAAGGTC TCATCCTTTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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