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VPS35 cDNA ORF clone, Homo sapiens (human)

Gene Symbol VPS35
Entrez Gene ID 55737
Full Name VPS35 retromer complex component
Synonyms MEM3, PARK17
General protein information
Preferred Names
vacuolar protein sorting-associated protein 35
Names
vacuolar protein sorting-associated protein 35
hVPS35
maternal-embryonic 3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

16

16q12

Summary This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

601501

Disorder Html:





The following VPS35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu69690 XM_011523227 PREDICTED: Homo sapiens vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 Quote Price
OHu49428 XM_005256045 PREDICTED: Homo sapiens vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 Quote Price
OHu18510 NM_018206 Homo sapiens VPS35 retromer complex component (VPS35), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$265.30
$379.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu69690
Accession Version XM_011523227.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product vacuolar protein sorting-associated protein 35 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGAAGAGAT GCCTGGACAA AAACAAGCTT ATGGATGCTC TAAAACATGC TTCTAATATG 
CTTGGTGAAC TCCGGACTTC TATGTTATCA CCAAAGAGTT ACTATGAACT TTATATGGCC
ATTTCTGATG AACTGCACTA CTTGGAGGTC TACCTGACAG ATGAGTTTGC TAAAGGAAGG
AAAGTGGCAG ATCTCTACGA ACTTGTACAG TATGCTGGAA ACATTATCCC AAGGCTTTAC
CTTTTGATCA CAGTTGGAGT TGTATATGTC AAGTCATTTC CTCAGTCCAG GAAGGATATT
TTGAAAGATT TGGTAGAAAT GTGCCGTGGT GTGCAACATC CCTTGAGGGG TCTGTTTCTT
CGAAATTACC TTCTTCAGTG TACCAGAAAT ATCTTACCTG ATGAAGGAGA GCCAACAGAT
GAAGAAACAA CTGGTGACAT CAGTGATTCC ATGGATTTTG TACTGCTCAA CTTTGCAGAA
ATGAACAAGC TCTGGGTGCG AATGCAGCAT CAGGGACATA GCCGAGATAG AGAAAAAAGA
GAACGAGAAA GACAAGAACT GAGAATTTTA GTGGGAACAA ATTTGGTGCG CCTCAGTCAG
TTGGAAGGTG TAAATGTGGA ACGTTACAAA CAGATTGTTT TGACTGGCAT ATTGGAGCAA
GTTGTAAACT GTAGGGATGC TTTGGCTCAA GAATATCTCA TGGAGTGTAT TATTCAGGTT
TTCCCTGATG AATTTCACCT CCAGACTTTG AATCCTTTTC TTCGGGCCTG TGCTGAGTTA
CACCAGAATG TAAATGTGAA GAACATAATC ATTGCTTTAA TTGATAGATT AGCTTTATTT
GCTCACCGTG AAGATGGACC TGGAATCCCA GCGGATATTA AACTTTTTGA TATATTTTCA
CAGCAGGTGG CTACAGTGAT ACAGTCTAGA CAAGACATGC CTTCAGAGGA TGTTGTATCT
TTACAAGTCT CTCTGATTAA TCTTGCCATG AAATGTTACC CTGATCGTGT GGACTATGTT
GATAAAGTTC TAGAAACAAC AGTGGAGATA TTCAATAAGC TCAACCTTGA ACATATTGCT
ACCAGTAGTG CAGTTTCAAA GGAACTCACC AGACTTTTGA AAATACCAGT TGACACTTAC
AACAATATTT TAACAGTCTT GAAATTAAAA CATTTTCACC CACTCTTTGA GTACTTTGAC
TACGAGTCCA GAAAGAGCAT GAGTTGTTAT GTGCTTAGTA ATGTTCTGGA TTATAACACA
GAAATTGTCT CTCAAGACCA GGTGGATTCC ATAATGAATT TGGTATCCAC GTTGATTCAA
GATCAGCCAG ATCAACCTGT AGAAGACCCT GATCCAGAAG ATTTTGCTGA TGAGCAGAGC
CTTGTGGGCC GCTTCATTCA TCTGCTGCGC TCTGAGGACC CTGACCAGCA GTACTTGATT
TTGAACACAG CACGAAAACA TTTTGGAGCT GGTGGAAATC AGCGGATTCG CTTCACACTG
CCACCTTTGG TATTTGCAGC TTACCAGCTG GCTTTTCGAT ATAAAGAGAA TTCTAAAGTG
GATGACAAAT GGGAAAAGAA ATGCCAGAAG ATTTTTTCAT TTGCCCACCA GACTATCAGT
GCTTTGATCA AAGCAGAGCT GGCAGAATTG CCCTTAAGAC TTTTTCTTCA AGGAGCACTA
GCTGCTGGGG AAATTGGTTT TGAAAATCAT GAGACAGTCG CATATGAATT CATGTCCCAG
GCATTTTCTC TGTATGAAGA TGAAATCAGC GATTCCAAAG CACAGCTAGC TGCCATCACC
TTGATCATTG GCACTTTTGA AAGGATGAAG TGCTTCAGTG AAGAGAATCA CGAACCTCTG
AGGACTCAGT GTGCCCTTGC TGCATCCAAA CTTCTAAAGA AACCTGATCA GGGCCGAGCT
GTGAGCACCT GTGCACATCT CTTCTGGTCT GGCAGAAACA CGGACAAAAA TGGGGAGGAG
CTTCACGGAG GCAAGAGGGT AATGGAGTGC CTAAAAAAAG CTCTAAAAAT AGCAAATCAG
TGCATGGACC CCTCTCTACA AGTGCAGCTT TTTATAGAAA TTCTGAACAG ATATATCTAT
TTTTATGAAA AGGAAAATGA TGCGGTAACA ATTCAGGTTT TAAACCAGCT TATCCAAAAG
ATTCGAGAAG ACCTCCCGAA TCTTGAATCC AGTGAAGAAA CAGAGCAGAT TAACAAACAT
TTTCATAACA CACTGGAGCA TTTGCGCTTG CGGCGGGAAT CACCAGAATC CGAGGGGCCA
ATTTATGAAG GTCTCATCCT TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011521529.1
CDS880..3183
Misc Feature(1)880..3054(+)
Translation
Position Chain Variation Link
2 2 a, g dbSNP:186169135
7 7 c, g dbSNP:370034762
13 13 c, t dbSNP:749720776
19 19 c, t dbSNP:778111576
22 22 c, t dbSNP:756674794
24 24 a, g dbSNP:750983665
28 28 c, g dbSNP:765926512
34 34 c, t dbSNP:757976749
35 35 c, g, t dbSNP:543764837
42 42 a, c, t dbSNP:761569610
51 51 c, t dbSNP:753702271
78 78 a, g dbSNP:763893039
81 81 c, t dbSNP:760678729
82 82 c, t dbSNP:753350623
83 83 c, g dbSNP:576387367
86 86 a, c dbSNP:557999797
100 100 a, g dbSNP:774693943
101 101 c, t dbSNP:771319720
103 103 a, c dbSNP:555565705
104 104 g, t dbSNP:539474321
116 116 a, g dbSNP:778222228
122 122 a, g dbSNP:770141298
125 125 a, g dbSNP:748691413
129 129 c, g dbSNP:781613448
135 135 -, gga dbSNP:747822188
158 158 a, c dbSNP:757888472
169 169 a, g dbSNP:572359965
170 170 a, g dbSNP:553986609
184 184 c, g dbSNP:535545021
217 217 g, t dbSNP:568362832
250 250 g, t dbSNP:147207677
259 259 a, g dbSNP:538181707
279 279 c, t dbSNP:571079336
281 281 g, t dbSNP:117884892
315 315 a, t dbSNP:532797701
348 348 c, t dbSNP:551083977
427 427 c, g dbSNP:144622564
442 442 c, t dbSNP:547559567
462 462 a, g dbSNP:190210064
501 501 g, t dbSNP:561896046
514 514 c, g dbSNP:185298087
526 526 a, g dbSNP:576161528
536 536 c, t dbSNP:140762726
548 548 g, t dbSNP:546040656
557 557 c, t dbSNP:147243765
558 558 a, g dbSNP:773872331
604 604 c, t dbSNP:554135407
657 657 -, aaa dbSNP:758671098
664 664 a, c dbSNP:550660542
677 677 c, t dbSNP:117389817
697 697 a, t dbSNP:561843305
701 701 a, g dbSNP:373624141
704 704 a, c dbSNP:556211504
709 709 a, g dbSNP:377291762
737 737 g, t dbSNP:367828156
743 743 a, g dbSNP:373500346
799 799 a, g dbSNP:774604183
825 825 a, g dbSNP:771229656
838 838 c, t dbSNP:542307781
842 842 a, t dbSNP:773381942
843 843 c, t dbSNP:770188899
848 848 c, g dbSNP:370411054
852 852 a, g dbSNP:781646463
856 856 a, g dbSNP:769187894
871 871 g, t dbSNP:745338373
881 881 a, t dbSNP:145147781
902 902 a, c, g dbSNP:777006799
936 936 c, t dbSNP:769097906
943 943 a, g dbSNP:193077277
952 952 c, t dbSNP:186122975
953 953 a, g dbSNP:770464667
960 960 c, t dbSNP:748800470
961 961 a, g, t dbSNP:375285388
963 963 a, g dbSNP:183554824
986 986 c, t dbSNP:11550464
1000 1000 a, t dbSNP:781057884
1004 1004 c, t dbSNP:754806601
1016 1016 a, g dbSNP:746848785
1017 1017 c, g dbSNP:529522862
1019 1019 a, g dbSNP:758294660
1022 1022 c, t dbSNP:11550462
1037 1037 c, g dbSNP:188245364
1040 1040 a, t dbSNP:12925313
1041 1041 a, t dbSNP:12920740
1058 1058 a, g dbSNP:750463506
1077 1077 c, t dbSNP:375122817
1083 1083 c, t dbSNP:757397775
1107 1107 a, c, g dbSNP:764351948
1119 1119 c, t dbSNP:768460283
1131 1131 a, g dbSNP:746758894
1170 1170 a, g, t dbSNP:141444850
1174 1174 a, g dbSNP:375986040
1244 1244 -, cag dbSNP:747313765
1245 1245 -, g dbSNP:780429873
1249 1249 -, agaaatatc dbSNP:758873324
1258 1258 c, t dbSNP:778970704
1263 1263 c, g dbSNP:757308043
1273 1273 c, t dbSNP:370380458
1276 1276 a, c dbSNP:777932822
1284 1284 a, g dbSNP:184863243
1288 1288 a, g dbSNP:756353898
1292 1292 a, c dbSNP:753047950
1302 1302 a, g dbSNP:770887209
1329 1329 a, c dbSNP:749320030
1342 1342 c, t dbSNP:373346821
1355 1355 a, c dbSNP:200388382
1368 1368 a, g dbSNP:756262411
1378 1378 c, t dbSNP:201098565
1395 1395 a, c dbSNP:781534743
1396 1396 a, c dbSNP:79423190
1401 1401 c, t dbSNP:755273544
1402 1402 a, c dbSNP:369424393
1407 1407 c, t dbSNP:766949427
1434 1434 a, g dbSNP:758993631
1437 1437 a, g dbSNP:753336800
1440 1440 a, g dbSNP:763697798
1449 1449 a, g dbSNP:760294326
1470 1470 c, t dbSNP:376845827
1471 1471 c, t dbSNP:142925902
1475 1475 c, g dbSNP:201996601
1483 1483 -, g dbSNP:35060795
1506 1506 c, t dbSNP:759216006
1515 1515 g, t dbSNP:192783364
1540 1540 a, g dbSNP:375809380
1554 1554 a, g dbSNP:766158776
1578 1578 c, t dbSNP:762793303
1588 1588 a, g dbSNP:773110975
1601 1601 g, t dbSNP:761821222
1602 1602 c, t dbSNP:776505532
1611 1611 a, g dbSNP:768773395
1622 1622 a, g dbSNP:760891909
1626 1626 c, t dbSNP:148572750
1633 1633 c, g dbSNP:772459263
1641 1641 g, t dbSNP:746190765
1642 1642 c, t dbSNP:779434158
1643 1643 a, g dbSNP:771276024
1656 1656 a, g dbSNP:749799639
1657 1657 g, t dbSNP:778203456
1660 1660 c, t dbSNP:754487190
1667 1667 a, g dbSNP:751107774
1675 1675 g, t dbSNP:779641969
1684 1684 a, g dbSNP:758068523
1704 1704 c, t dbSNP:750157630
1706 1706 a, g dbSNP:371218680
1707 1707 a, t dbSNP:77133510
1710 1710 a, t dbSNP:756137934
1722 1722 c, t dbSNP:752758821
1726 1726 c, t dbSNP:767701361
1727 1727 a, g dbSNP:759749769
1731 1731 a, g dbSNP:200578690
1735 1735 a, g dbSNP:774611405
1737 1737 a, t dbSNP:144346159
1738 1738 c, t dbSNP:770029606
1739 1739 c, g, t dbSNP:770226984
1740 1740 c, t dbSNP:748655024
1747 1747 c, t dbSNP:777184680
1751 1751 c, t dbSNP:747944333
1752 1752 a, g dbSNP:745416544
1754 1754 a, g dbSNP:778590455
1755 1755 c, t dbSNP:756884549
1758 1758 g, t dbSNP:749058909
1765 1765 c, t dbSNP:777437956
1770 1770 c, t dbSNP:756040992
1771 1771 a, g dbSNP:752668855
1780 1780 c, t dbSNP:767613694
1812 1812 a, g dbSNP:188883365
1827 1827 a, g dbSNP:563975281
1834 1834 a, g dbSNP:140265194
1848 1848 c, t dbSNP:769692526
1851 1851 c, t dbSNP:747997807
1867 1867 a, g dbSNP:781234198
1872 1872 a, g dbSNP:754892097
1873 1873 g, t dbSNP:751599316
1879 1879 c, t dbSNP:780001713
1885 1885 c, t dbSNP:758568605
1886 1886 a, g dbSNP:201593831
1902 1902 c, t dbSNP:113154062
1911 1911 a, g dbSNP:765489808
1916 1916 a, c dbSNP:762131827
1930 1930 c, t dbSNP:754226798
1934 1934 a, g, t dbSNP:754978683
1937 1937 a, g dbSNP:116254156
1940 1940 c, t dbSNP:770335442
1943 1943 a, g dbSNP:762573165
1944 1944 c, t dbSNP:772970332
1962 1962 c, t dbSNP:757485744
1968 1968 -, agt dbSNP:779224141
1972 1972 a, g dbSNP:754137147
1987 1987 a, g dbSNP:764365507
2021 2021 a, g dbSNP:756559738
2047 2047 a, t dbSNP:370055356
2054 2054 a, t dbSNP:375087873
2056 2056 c, t dbSNP:762332686
2060 2060 c, t dbSNP:373075116
2069 2069 a, c dbSNP:764924463
2076 2076 c, t dbSNP:530665086
2082 2082 c, t dbSNP:368876504
2083 2083 a, g, t dbSNP:143688112
2084 2084 a, t dbSNP:139200616
2089 2089 a, c dbSNP:191165923
2105 2105 a, g dbSNP:745893720
2109 2109 c, t dbSNP:187425753
2119 2119 a, g dbSNP:376209552
2125 2125 c, g dbSNP:749503515
2132 2132 a, t dbSNP:778105954
2136 2136 c, t dbSNP:756404549
2145 2145 c, t dbSNP:753137936
2150 2150 c, t dbSNP:17850640
2154 2154 a, g dbSNP:767864451
2170 2170 a, g dbSNP:367611000
2186 2186 c, g dbSNP:748396803
2189 2189 c, t dbSNP:781327081
2190 2190 a, g dbSNP:150810185
2195 2195 c, t dbSNP:747414987
2199 2199 a, g dbSNP:780663505
2208 2208 a, t dbSNP:374074473
2212 2212 c, g dbSNP:370401767
2218 2218 g, t dbSNP:376308396
2219 2219 c, t dbSNP:373113084
2220 2220 a, g dbSNP:528116722
2226 2226 c, g dbSNP:767202087
2227 2227 a, c dbSNP:759288056
2239 2239 g, t dbSNP:751379541
2260 2260 c, t dbSNP:567251715
2263 2263 a, g dbSNP:766248974
2265 2265 a, g dbSNP:762949831
2267 2267 a, g dbSNP:747880925
2268 2268 c, t dbSNP:773089248
2272 2272 a, t dbSNP:141253289
2275 2275 a, t dbSNP:761848190
2280 2280 c, t dbSNP:776877370
2286 2286 a, g dbSNP:769027812
2287 2287 c, t dbSNP:747325352
2288 2288 a, g dbSNP:780577406
2289 2289 c, g dbSNP:772634549
2295 2295 a, g dbSNP:267604550
2296 2296 g, t dbSNP:746375559
2308 2308 a, c dbSNP:779340678
2309 2309 a, t dbSNP:755622603
2325 2325 c, t dbSNP:373955385
2327 2327 c, t dbSNP:757154329
2362 2362 c, t dbSNP:184277092
2367 2367 a, t dbSNP:753900468
2368 2368 c, t dbSNP:398124658
2378 2378 a, t dbSNP:760887864
2382 2382 a, g dbSNP:775824787
2383 2383 c, t dbSNP:369196817
2394 2394 c, t dbSNP:376718984
2406 2406 g, t dbSNP:759900117
2428 2428 a, c dbSNP:774595498
2433 2433 c, t dbSNP:771405301
2434 2434 a, g dbSNP:749793948
2437 2437 c, g dbSNP:568160306
2440 2440 c, g dbSNP:748389084
2443 2443 a, g dbSNP:779063544
2445 2445 c, t dbSNP:533283266
2461 2461 a, t dbSNP:13332463
2469 2469 g, t dbSNP:770312643
2471 2471 c, t dbSNP:760128592
2494 2494 a, g dbSNP:774875351
2495 2495 c, t dbSNP:376786968
2533 2533 c, t dbSNP:745500235
2538 2538 a, g dbSNP:778678166
2568 2568 c, g dbSNP:770819201
2571 2571 a, t dbSNP:749127069
2575 2575 a, c, g dbSNP:201566773
2587 2587 c, g dbSNP:148255605
2592 2592 a, g dbSNP:752760936
2595 2595 a, g dbSNP:145679337
2597 2597 a, t dbSNP:34687100
2598 2598 c, t dbSNP:755145722
2601 2601 a, g, t dbSNP:142063738
2649 2649 c, t dbSNP:758632241
2650 2650 a, g dbSNP:188286943
2669 2669 c, t dbSNP:201998902
2673 2673 c, t dbSNP:138794859
2675 2675 c, t dbSNP:757674131
2683 2683 a, c dbSNP:754270736
2686 2686 a, g dbSNP:764695026
2687 2687 c, t dbSNP:756550058
2693 2693 c, g dbSNP:758922774
2697 2697 g, t dbSNP:569735490
2704 2704 a, t dbSNP:144997996
2730 2730 c, t dbSNP:168745
2737 2737 c, t dbSNP:145406665
2748 2748 a, g dbSNP:769692699
2763 2763 a, g dbSNP:748012697
2766 2766 c, t dbSNP:559637051
2768 2768 a, g dbSNP:776389848
2799 2799 c, t dbSNP:376700700
2808 2808 a, c dbSNP:746999621
2812 2812 g, t dbSNP:780111914
2814 2814 a, g dbSNP:375643041
2826 2826 g, t dbSNP:758539394
2827 2827 c, t dbSNP:547369855
2839 2839 a, g dbSNP:779153929
2841 2841 a, g dbSNP:757523799
2846 2846 a, g dbSNP:754253874
2850 2850 g, t dbSNP:764451293
2853 2853 c, g dbSNP:188831675
2855 2855 a, c, g dbSNP:765978232
2860 2860 a, c dbSNP:150494360
2865 2865 c, t dbSNP:542139125
2870 2870 c, g dbSNP:753236884
2871 2871 c, t dbSNP:781775490
2874 2874 a, g dbSNP:757938445
2877 2877 a, g dbSNP:573618693
2892 2892 a, t dbSNP:764734893
2908 2908 a, c dbSNP:761527195
2914 2914 a, c dbSNP:199940967
2915 2915 a, g dbSNP:375501847
2930 2930 c, g dbSNP:760434383
2937 2937 a, g dbSNP:145033509
2941 2941 a, g dbSNP:184960717
2943 2943 a, g dbSNP:772021168
2973 2973 c, t dbSNP:369831560
2978 2978 a, c dbSNP:774419073
3001 3001 c, g dbSNP:557875371
3002 3002 c, t dbSNP:749516404
3014 3014 a, c dbSNP:547805228
3026 3026 a, t dbSNP:747505018
3033 3033 c, t dbSNP:140543776
3055 3055 c, t dbSNP:76693948
3056 3056 a, c, t dbSNP:80303950
3061 3061 c, t dbSNP:113746010
3079 3079 a, g dbSNP:370966099
3098 3098 a, g dbSNP:777427799
3103 3103 c, g dbSNP:755770851
3108 3108 c, t dbSNP:752414695
3112 3112 a, c dbSNP:192419029
3117 3117 c, g dbSNP:754820224
3124 3124 c, t dbSNP:751488260
3125 3125 a, g dbSNP:532641584
3130 3130 c, g, t dbSNP:768025111
3136 3136 a, g dbSNP:763043556
3138 3138 a, g dbSNP:181292654
3139 3139 a, t dbSNP:773175370
3146 3146 a, t dbSNP:765487058
3150 3150 c, t dbSNP:762118625
3151 3151 a, g dbSNP:777050595
3153 3153 a, g dbSNP:184968916
3156 3156 g, t dbSNP:769119901
3168 3168 a, g dbSNP:747417009
3174 3174 c, t dbSNP:776023975
3185 3185 a, g dbSNP:200195623
3187 3187 c, g dbSNP:570492580
3190 3190 a, g dbSNP:183923278
3191 3191 a, g dbSNP:188654478
3194 3194 a, g dbSNP:189948577
3201 3201 a, g dbSNP:780867284
3204 3204 c, g dbSNP:754730255
3210 3210 g, t dbSNP:751335821
3217 3217 a, g dbSNP:779980787
3219 3219 a, g dbSNP:758270070
3224 3224 g, t dbSNP:750436964
3226 3226 a, g dbSNP:765392455
3237 3237 a, t dbSNP:527800239
3239 3239 c, t dbSNP:185420432
3240 3240 a, g dbSNP:548502981
3256 3256 a, g dbSNP:112747048
3260 3260 a, g dbSNP:530305374
3330 3330 c, t dbSNP:759782658
3331 3331 a, g dbSNP:774726845
3350 3350 c, t dbSNP:563219537
3362 3362 a, g dbSNP:11550461
3365 3365 c, g dbSNP:759445626
3400 3400 a, g dbSNP:544896659
3442 3442 -, a, aa, aaa, aaaa dbSNP:59370839
3444 3444 -, aaa dbSNP:71852726
3461 3461 -, aaaaacaa, caaaaaaa dbSNP:575317929
3463 3463 -, aaa dbSNP:752834205
3464 3464 a, c dbSNP:808078
3464 3464 -, c dbSNP:373111906
3465 3465 -, aaa dbSNP:369756092
3465 3465 a, c dbSNP:71380964
3466 3466 a, c dbSNP:199765664
3469 3469 a, t dbSNP:1053393
3470 3470 -, aa dbSNP:369068093
3472 3472 c, t dbSNP:145606241
3526 3526 -, tttg dbSNP:564951006
3529 3529 a, g dbSNP:545755034
3537 3537 c, g dbSNP:572048744
3566 3566 a, g dbSNP:776500632
3583 3583 c, t dbSNP:543740178
3584 3584 a, g dbSNP:553790361
3605 3605 a, c dbSNP:181198454
3637 3637 c, t dbSNP:188743379
3684 3684 a, g dbSNP:774565598
3758 3758 a, t dbSNP:768759859
3766 3766 c, t dbSNP:542974709
3767 3767 g, t dbSNP:780922363
3770 3770 a, g dbSNP:754785560
3795 3795 c, t dbSNP:762727734
3800 3800 a, g dbSNP:746602580
3808 3808 a, t dbSNP:779756007
3827 3827 c, t dbSNP:749705519
3883 3883 a, t dbSNP:11550460
3922 3922 a, g dbSNP:758047233
3944 3944 -, t dbSNP:34926621
3945 3945 -, t dbSNP:544973710
3947 3947 c, t dbSNP:183646374
3997 3997 c, g dbSNP:575736800
4009 4009 c, t dbSNP:191441368
4027 4027 a, t dbSNP:1698957
4043 4043 a, g dbSNP:780263080
4064 4064 c, t dbSNP:560695968
4136 4136 c, t dbSNP:141839860
4168 4168 c, g dbSNP:542697419
4171 4171 c, g dbSNP:552312462
4222 4222 c, t dbSNP:794557
4264 4264 a, t dbSNP:79050797
4265 4265 c, t dbSNP:138252903
4288 4288 a, g dbSNP:145628294
4291 4291 c, t dbSNP:553910416
4292 4292 c, t dbSNP:112150674
4377 4377 c, g dbSNP:530343884
4400 4400 a, g dbSNP:115810403
4427 4427 c, t dbSNP:76259065
4429 4429 c, t dbSNP:148961435
4541 4541 a, g dbSNP:188470349
4563 4563 c, t dbSNP:545490118
4587 4587 g, t dbSNP:746409661
4661 4661 a, c dbSNP:539333964
4673 4673 -, t dbSNP:749537563
4702 4702 a, g dbSNP:571998815
4724 4724 a, g dbSNP:560037141
4731 4731 a, g dbSNP:794558
4741 4741 a, g dbSNP:774644453
4753 4753 c, g dbSNP:112333048
4768 4768 c, t dbSNP:555956898
4770 4770 a, t dbSNP:375977006
4782 4782 c, t dbSNP:763130659
4795 4795 -, c dbSNP:568752537
4799 4799 c, t dbSNP:371605094
4811 4811 c, g dbSNP:773307805
4827 4827 a, g dbSNP:537393708
4863 4863 c, t dbSNP:748025305
4868 4868 c, t dbSNP:11647138
4929 4929 a, c dbSNP:558779814
4967 4967 a, g dbSNP:768650421
4976 4976 a, g dbSNP:533970325
5021 5021 c, t dbSNP:566858054
5032 5032 a, c dbSNP:779764620
5066 5066 c, t dbSNP:794559
5106 5106 c, t dbSNP:374027606
5152 5152 a, c dbSNP:570059770
5220 5220 a, g dbSNP:548431200
5225 5225 c, t dbSNP:114654822
5236 5236 -, t dbSNP:200961009
5260 5260 a, g dbSNP:536731227
5272 5272 a, g dbSNP:752584142
5284 5284 g, t dbSNP:150247715
5285 5285 a, g dbSNP:112939943
5300 5300 g, t dbSNP:547249927
5304 5304 c, t dbSNP:527341985
5311 5311 c, t dbSNP:560084002
5350 5350 c, g dbSNP:753188016
5432 5432 a, g dbSNP:541382201
5445 5445 c, t dbSNP:184888157
5502 5502 a, g dbSNP:781763237
5513 5513 -, c dbSNP:780126752
5539 5539 -, a dbSNP:746904233
5637 5637 a, t dbSNP:755598539
5649 5649 c, t dbSNP:193290216

Target ORF information:

RefSeq Version XM_011523227
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011523227

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGAAGAGAT GCCTGGACAA AAACAAGCTT ATGGATGCTC TAAAACATGC TTCTAATATG 
CTTGGTGAAC TCCGGACTTC TATGTTATCA CCAAAGAGTT ACTATGAACT TTATATGGCC
ATTTCTGATG AACTGCACTA CTTGGAGGTC TACCTGACAG ATGAGTTTGC TAAAGGAAGG
AAAGTGGCAG ATCTCTACGA ACTTGTACAG TATGCTGGAA ACATTATCCC AAGGCTTTAC
CTTTTGATCA CAGTTGGAGT TGTATATGTC AAGTCATTTC CTCAGTCCAG GAAGGATATT
TTGAAAGATT TGGTAGAAAT GTGCCGTGGT GTGCAACATC CCTTGAGGGG TCTGTTTCTT
CGAAATTACC TTCTTCAGTG TACCAGAAAT ATCTTACCTG ATGAAGGAGA GCCAACAGAT
GAAGAAACAA CTGGTGACAT CAGTGATTCC ATGGATTTTG TACTGCTCAA CTTTGCAGAA
ATGAACAAGC TCTGGGTGCG AATGCAGCAT CAGGGACATA GCCGAGATAG AGAAAAAAGA
GAACGAGAAA GACAAGAACT GAGAATTTTA GTGGGAACAA ATTTGGTGCG CCTCAGTCAG
TTGGAAGGTG TAAATGTGGA ACGTTACAAA CAGATTGTTT TGACTGGCAT ATTGGAGCAA
GTTGTAAACT GTAGGGATGC TTTGGCTCAA GAATATCTCA TGGAGTGTAT TATTCAGGTT
TTCCCTGATG AATTTCACCT CCAGACTTTG AATCCTTTTC TTCGGGCCTG TGCTGAGTTA
CACCAGAATG TAAATGTGAA GAACATAATC ATTGCTTTAA TTGATAGATT AGCTTTATTT
GCTCACCGTG AAGATGGACC TGGAATCCCA GCGGATATTA AACTTTTTGA TATATTTTCA
CAGCAGGTGG CTACAGTGAT ACAGTCTAGA CAAGACATGC CTTCAGAGGA TGTTGTATCT
TTACAAGTCT CTCTGATTAA TCTTGCCATG AAATGTTACC CTGATCGTGT GGACTATGTT
GATAAAGTTC TAGAAACAAC AGTGGAGATA TTCAATAAGC TCAACCTTGA ACATATTGCT
ACCAGTAGTG CAGTTTCAAA GGAACTCACC AGACTTTTGA AAATACCAGT TGACACTTAC
AACAATATTT TAACAGTCTT GAAATTAAAA CATTTTCACC CACTCTTTGA GTACTTTGAC
TACGAGTCCA GAAAGAGCAT GAGTTGTTAT GTGCTTAGTA ATGTTCTGGA TTATAACACA
GAAATTGTCT CTCAAGACCA GGTGGATTCC ATAATGAATT TGGTATCCAC GTTGATTCAA
GATCAGCCAG ATCAACCTGT AGAAGACCCT GATCCAGAAG ATTTTGCTGA TGAGCAGAGC
CTTGTGGGCC GCTTCATTCA TCTGCTGCGC TCTGAGGACC CTGACCAGCA GTACTTGATT
TTGAACACAG CACGAAAACA TTTTGGAGCT GGTGGAAATC AGCGGATTCG CTTCACACTG
CCACCTTTGG TATTTGCAGC TTACCAGCTG GCTTTTCGAT ATAAAGAGAA TTCTAAAGTG
GATGACAAAT GGGAAAAGAA ATGCCAGAAG ATTTTTTCAT TTGCCCACCA GACTATCAGT
GCTTTGATCA AAGCAGAGCT GGCAGAATTG CCCTTAAGAC TTTTTCTTCA AGGAGCACTA
GCTGCTGGGG AAATTGGTTT TGAAAATCAT GAGACAGTCG CATATGAATT CATGTCCCAG
GCATTTTCTC TGTATGAAGA TGAAATCAGC GATTCCAAAG CACAGCTAGC TGCCATCACC
TTGATCATTG GCACTTTTGA AAGGATGAAG TGCTTCAGTG AAGAGAATCA CGAACCTCTG
AGGACTCAGT GTGCCCTTGC TGCATCCAAA CTTCTAAAGA AACCTGATCA GGGCCGAGCT
GTGAGCACCT GTGCACATCT CTTCTGGTCT GGCAGAAACA CGGACAAAAA TGGGGAGGAG
CTTCACGGAG GCAAGAGGGT AATGGAGTGC CTAAAAAAAG CTCTAAAAAT AGCAAATCAG
TGCATGGACC CCTCTCTACA AGTGCAGCTT TTTATAGAAA TTCTGAACAG ATATATCTAT
TTTTATGAAA AGGAAAATGA TGCGGTAACA ATTCAGGTTT TAAACCAGCT TATCCAAAAG
ATTCGAGAAG ACCTCCCGAA TCTTGAATCC AGTGAAGAAA CAGAGCAGAT TAACAAACAT
TTTCATAACA CACTGGAGCA TTTGCGCTTG CGGCGGGAAT CACCAGAATC CGAGGGGCCA
ATTTATGAAG GTCTCATCCT TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu49428
Accession Version XM_005256045.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product vacuolar protein sorting-associated protein 35 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010498.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530424104. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCCATTT CTGATGAACT GCACTACTTG GAGGTCTACC TGACAGATGA GTTTGCTAAA 
GGAAGGAAAG TGGCAGATCT CTACGAACTT GTACAGTATG CTGGAAACAT TATCCCAAGG
CTTTACCTTT TGATCACAGT TGGAGTTGTA TATGTCAAGT CATTTCCTCA GTCCAGGAAG
GATATTTTGA AAGATTTGGT AGAAATGTGC CGTGGTGTGC AACATCCCTT GAGGGGTCTG
TTTCTTCGAA ATTACCTTCT TCAGTGTACC AGAAATATCT TACCTGATGA AGGAGAGCCA
ACAGATGAAG AAACAACTGG TGACATCAGT GATTCCATGG ATTTTGTACT GCTCAACTTT
GCAGAAATGA ACAAGCTCTG GGTGCGAATG CAGCATCAGG GACATAGCCG AGATAGAGAA
AAAAGAGAAC GAGAAAGACA AGAACTGAGA ATTTTAGTGG GAACAAATTT GGTGCGCCTC
AGTCAGTTGG AAGGTGTAAA TGTGGAACGT TACAAACAGA TTGTTTTGAC TGGCATATTG
GAGCAAGTTG TAAACTGTAG GGATGCTTTG GCTCAAGAAT ATCTCATGGA GTGTATTATT
CAGGTTTTCC CTGATGAATT TCACCTCCAG ACTTTGAATC CTTTTCTTCG GGCCTGTGCT
GAGTTACACC AGAATGTAAA TGTGAAGAAC ATAATCATTG CTTTAATTGA TAGATTAGCT
TTATTTGCTC ACCGTGAAGA TGGACCTGGA ATCCCAGCGG ATATTAAACT TTTTGATATA
TTTTCACAGC AGGTGGCTAC AGTGATACAG TCTAGACAAG ACATGCCTTC AGAGGATGTT
GTATCTTTAC AAGTCTCTCT GATTAATCTT GCCATGAAAT GTTACCCTGA TCGTGTGGAC
TATGTTGATA AAGTTCTAGA AACAACAGTG GAGATATTCA ATAAGCTCAA CCTTGAACAT
ATTGCTACCA GTAGTGCAGT TTCAAAGGAA CTCACCAGAC TTTTGAAAAT ACCAGTTGAC
ACTTACAACA ATATTTTAAC AGTCTTGAAA TTAAAACATT TTCACCCACT CTTTGAGTAC
TTTGACTACG AGTCCAGAAA GAGCATGAGT TGTTATGTGC TTAGTAATGT TCTGGATTAT
AACACAGAAA TTGTCTCTCA AGACCAGGTG GATTCCATAA TGAATTTGGT ATCCACGTTG
ATTCAAGATC AGCCAGATCA ACCTGTAGAA GACCCTGATC CAGAAGATTT TGCTGATGAG
CAGAGCCTTG TGGGCCGCTT CATTCATCTG CTGCGCTCTG AGGACCCTGA CCAGCAGTAC
TTGATTTTGA ACACAGCACG AAAACATTTT GGAGCTGGTG GAAATCAGCG GATTCGCTTC
ACACTGCCAC CTTTGGTATT TGCAGCTTAC CAGCTGGCTT TTCGATATAA AGAGAATTCT
AAAGTGGATG ACAAATGGGA AAAGAAATGC CAGAAGATTT TTTCATTTGC CCACCAGACT
ATCAGTGCTT TGATCAAAGC AGAGCTGGCA GAATTGCCCT TAAGACTTTT TCTTCAAGGA
GCACTAGCTG CTGGGGAAAT TGGTTTTGAA AATCATGAGA CAGTCGCATA TGAATTCATG
TCCCAGGCAT TTTCTCTGTA TGAAGATGAA ATCAGCGATT CCAAAGCACA GCTAGCTGCC
ATCACCTTGA TCATTGGCAC TTTTGAAAGG ATGAAGTGCT TCAGTGAAGA GAATCACGAA
CCTCTGAGGA CTCAGTGTGC CCTTGCTGCA TCCAAACTTC TAAAGAAACC TGATCAGGGC
CGAGCTGTGA GCACCTGTGC ACATCTCTTC TGGTCTGGCA GAAACACGGA CAAAAATGGG
GAGGAGCTTC ACGGAGGCAA GAGGGTAATG GAGTGCCTAA AAAAAGCTCT AAAAATAGCA
AATCAGTGCA TGGACCCCTC TCTACAAGTG CAGCTTTTTA TAGAAATTCT GAACAGATAT
ATCTATTTTT ATGAAAAGGA AAATGATGCG GTAACAATTC AGGTTTTAAA CCAGCTTATC
CAAAAGATTC GAGAAGACCT CCCGAATCTT GAATCCAGTG AAGAAACAGA GCAGATTAAC
AAACATTTTC ATAACACACT GGAGCATTTG CGCTTGCGGC GGGAATCACC AGAATCCGAG
GGGCCAATTT ATGAAGGTCT CATCCTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_005256102.1
CDS186..2375
Misc Feature(1)186..2246(+)
Translation
Position Chain Variation Link
10 10 a, g dbSNP:761175510
13 13 c, g dbSNP:773461813
17 17 a, g dbSNP:767874057
20 20 a, c dbSNP:762053892
21 21 a, g dbSNP:774526654
23 23 g, t dbSNP:768774857
26 26 c, g dbSNP:749358646
27 27 c, t dbSNP:398122321
27 27 -, t dbSNP:772743562
27 27 c, g, t dbSNP:189775907
28 28 a, c, g, t dbSNP:3743928
32 32 a, g dbSNP:746704070
33 33 c, t dbSNP:182309233
38 38 c, t dbSNP:541080284
39 39 a, g dbSNP:758046563
40 40 c, t dbSNP:752258031
43 43 a, c, t dbSNP:573920049
44 44 a, c, g dbSNP:767812765
46 46 c, g dbSNP:762144323
47 47 a, c dbSNP:774616523
49 49 c, t dbSNP:764298631
52 52 g, t dbSNP:763096994
53 53 c, g dbSNP:778763088
58 58 c, t dbSNP:775449983
68 68 a, g dbSNP:774604183
94 94 a, g dbSNP:771229656
107 107 c, t dbSNP:542307781
111 111 a, t dbSNP:773381942
112 112 c, t dbSNP:770188899
117 117 c, g dbSNP:370411054
121 121 a, g dbSNP:781646463
125 125 a, g dbSNP:769187894
140 140 g, t dbSNP:745338373
150 150 a, t dbSNP:145147781
173 173 a, g dbSNP:377328564
176 176 a, c dbSNP:372024197
177 177 c, t dbSNP:547732314
192 192 a, t dbSNP:781057884
196 196 c, t dbSNP:754806601
208 208 a, g dbSNP:746848785
209 209 c, g dbSNP:529522862
211 211 a, g dbSNP:758294660
214 214 c, t dbSNP:11550462
229 229 c, g dbSNP:188245364
232 232 a, t dbSNP:12925313
233 233 a, t dbSNP:12920740
250 250 a, g dbSNP:750463506
269 269 c, t dbSNP:375122817
275 275 c, t dbSNP:757397775
299 299 a, c, g dbSNP:764351948
311 311 c, t dbSNP:768460283
323 323 a, g dbSNP:746758894
362 362 a, g, t dbSNP:141444850
366 366 a, g dbSNP:375986040
436 436 -, cag dbSNP:747313765
437 437 -, g dbSNP:780429873
441 441 -, agaaatatc dbSNP:758873324
450 450 c, t dbSNP:778970704
455 455 c, g dbSNP:757308043
465 465 c, t dbSNP:370380458
468 468 a, c dbSNP:777932822
476 476 a, g dbSNP:184863243
480 480 a, g dbSNP:756353898
484 484 a, c dbSNP:753047950
494 494 a, g dbSNP:770887209
521 521 a, c dbSNP:749320030
534 534 c, t dbSNP:373346821
547 547 a, c dbSNP:200388382
560 560 a, g dbSNP:756262411
570 570 c, t dbSNP:201098565
587 587 a, c dbSNP:781534743
588 588 a, c dbSNP:79423190
593 593 c, t dbSNP:755273544
594 594 a, c dbSNP:369424393
599 599 c, t dbSNP:766949427
626 626 a, g dbSNP:758993631
629 629 a, g dbSNP:753336800
632 632 a, g dbSNP:763697798
641 641 a, g dbSNP:760294326
662 662 c, t dbSNP:376845827
663 663 c, t dbSNP:142925902
667 667 c, g dbSNP:201996601
675 675 -, g dbSNP:35060795
698 698 c, t dbSNP:759216006
707 707 g, t dbSNP:192783364
732 732 a, g dbSNP:375809380
746 746 a, g dbSNP:766158776
770 770 c, t dbSNP:762793303
780 780 a, g dbSNP:773110975
793 793 g, t dbSNP:761821222
794 794 c, t dbSNP:776505532
803 803 a, g dbSNP:768773395
814 814 a, g dbSNP:760891909
818 818 c, t dbSNP:148572750
825 825 c, g dbSNP:772459263
833 833 g, t dbSNP:746190765
834 834 c, t dbSNP:779434158
835 835 a, g dbSNP:771276024
848 848 a, g dbSNP:749799639
849 849 g, t dbSNP:778203456
852 852 c, t dbSNP:754487190
859 859 a, g dbSNP:751107774
867 867 g, t dbSNP:779641969
876 876 a, g dbSNP:758068523
896 896 c, t dbSNP:750157630
898 898 a, g dbSNP:371218680
899 899 a, t dbSNP:77133510
902 902 a, t dbSNP:756137934
914 914 c, t dbSNP:752758821
918 918 c, t dbSNP:767701361
919 919 a, g dbSNP:759749769
923 923 a, g dbSNP:200578690
927 927 a, g dbSNP:774611405
929 929 a, t dbSNP:144346159
930 930 c, t dbSNP:770029606
931 931 c, g, t dbSNP:770226984
932 932 c, t dbSNP:748655024
939 939 c, t dbSNP:777184680
943 943 c, t dbSNP:747944333
944 944 a, g dbSNP:745416544
946 946 a, g dbSNP:778590455
947 947 c, t dbSNP:756884549
950 950 g, t dbSNP:749058909
957 957 c, t dbSNP:777437956
962 962 c, t dbSNP:756040992
963 963 a, g dbSNP:752668855
972 972 c, t dbSNP:767613694
1004 1004 a, g dbSNP:188883365
1019 1019 a, g dbSNP:563975281
1026 1026 a, g dbSNP:140265194
1040 1040 c, t dbSNP:769692526
1043 1043 c, t dbSNP:747997807
1059 1059 a, g dbSNP:781234198
1064 1064 a, g dbSNP:754892097
1065 1065 g, t dbSNP:751599316
1071 1071 c, t dbSNP:780001713
1077 1077 c, t dbSNP:758568605
1078 1078 a, g dbSNP:201593831
1094 1094 c, t dbSNP:113154062
1103 1103 a, g dbSNP:765489808
1108 1108 a, c dbSNP:762131827
1122 1122 c, t dbSNP:754226798
1126 1126 a, g, t dbSNP:754978683
1129 1129 a, g dbSNP:116254156
1132 1132 c, t dbSNP:770335442
1135 1135 a, g dbSNP:762573165
1136 1136 c, t dbSNP:772970332
1154 1154 c, t dbSNP:757485744
1160 1160 -, agt dbSNP:779224141
1164 1164 a, g dbSNP:754137147
1179 1179 a, g dbSNP:764365507
1213 1213 a, g dbSNP:756559738
1239 1239 a, t dbSNP:370055356
1246 1246 a, t dbSNP:375087873
1248 1248 c, t dbSNP:762332686
1252 1252 c, t dbSNP:373075116
1261 1261 a, c dbSNP:764924463
1268 1268 c, t dbSNP:530665086
1274 1274 c, t dbSNP:368876504
1275 1275 a, g, t dbSNP:143688112
1276 1276 a, t dbSNP:139200616
1281 1281 a, c dbSNP:191165923
1297 1297 a, g dbSNP:745893720
1301 1301 c, t dbSNP:187425753
1311 1311 a, g dbSNP:376209552
1317 1317 c, g dbSNP:749503515
1324 1324 a, t dbSNP:778105954
1328 1328 c, t dbSNP:756404549
1337 1337 c, t dbSNP:753137936
1342 1342 c, t dbSNP:17850640
1346 1346 a, g dbSNP:767864451
1362 1362 a, g dbSNP:367611000
1378 1378 c, g dbSNP:748396803
1381 1381 c, t dbSNP:781327081
1382 1382 a, g dbSNP:150810185
1387 1387 c, t dbSNP:747414987
1391 1391 a, g dbSNP:780663505
1400 1400 a, t dbSNP:374074473
1404 1404 c, g dbSNP:370401767
1410 1410 g, t dbSNP:376308396
1411 1411 c, t dbSNP:373113084
1412 1412 a, g dbSNP:528116722
1418 1418 c, g dbSNP:767202087
1419 1419 a, c dbSNP:759288056
1431 1431 g, t dbSNP:751379541
1452 1452 c, t dbSNP:567251715
1455 1455 a, g dbSNP:766248974
1457 1457 a, g dbSNP:762949831
1459 1459 a, g dbSNP:747880925
1460 1460 c, t dbSNP:773089248
1464 1464 a, t dbSNP:141253289
1467 1467 a, t dbSNP:761848190
1472 1472 c, t dbSNP:776877370
1478 1478 a, g dbSNP:769027812
1479 1479 c, t dbSNP:747325352
1480 1480 a, g dbSNP:780577406
1481 1481 c, g dbSNP:772634549
1487 1487 a, g dbSNP:267604550
1488 1488 g, t dbSNP:746375559
1500 1500 a, c dbSNP:779340678
1501 1501 a, t dbSNP:755622603
1517 1517 c, t dbSNP:373955385
1519 1519 c, t dbSNP:757154329
1554 1554 c, t dbSNP:184277092
1559 1559 a, t dbSNP:753900468
1560 1560 c, t dbSNP:398124658
1570 1570 a, t dbSNP:760887864
1574 1574 a, g dbSNP:775824787
1575 1575 c, t dbSNP:369196817
1586 1586 c, t dbSNP:376718984
1598 1598 g, t dbSNP:759900117
1620 1620 a, c dbSNP:774595498
1625 1625 c, t dbSNP:771405301
1626 1626 a, g dbSNP:749793948
1629 1629 c, g dbSNP:568160306
1632 1632 c, g dbSNP:748389084
1635 1635 a, g dbSNP:779063544
1637 1637 c, t dbSNP:533283266
1653 1653 a, t dbSNP:13332463
1661 1661 g, t dbSNP:770312643
1663 1663 c, t dbSNP:760128592
1686 1686 a, g dbSNP:774875351
1687 1687 c, t dbSNP:376786968
1725 1725 c, t dbSNP:745500235
1730 1730 a, g dbSNP:778678166
1760 1760 c, g dbSNP:770819201
1763 1763 a, t dbSNP:749127069
1767 1767 a, c, g dbSNP:201566773
1779 1779 c, g dbSNP:148255605
1784 1784 a, g dbSNP:752760936
1787 1787 a, g dbSNP:145679337
1789 1789 a, t dbSNP:34687100
1790 1790 c, t dbSNP:755145722
1793 1793 a, g, t dbSNP:142063738
1841 1841 c, t dbSNP:758632241
1842 1842 a, g dbSNP:188286943
1861 1861 c, t dbSNP:201998902
1865 1865 c, t dbSNP:138794859
1867 1867 c, t dbSNP:757674131
1875 1875 a, c dbSNP:754270736
1878 1878 a, g dbSNP:764695026
1879 1879 c, t dbSNP:756550058
1885 1885 c, g dbSNP:758922774
1889 1889 g, t dbSNP:569735490
1896 1896 a, t dbSNP:144997996
1922 1922 c, t dbSNP:168745
1929 1929 c, t dbSNP:145406665
1940 1940 a, g dbSNP:769692699
1955 1955 a, g dbSNP:748012697
1958 1958 c, t dbSNP:559637051
1960 1960 a, g dbSNP:776389848
1991 1991 c, t dbSNP:376700700
2000 2000 a, c dbSNP:746999621
2004 2004 g, t dbSNP:780111914
2006 2006 a, g dbSNP:375643041
2018 2018 g, t dbSNP:758539394
2019 2019 c, t dbSNP:547369855
2031 2031 a, g dbSNP:779153929
2033 2033 a, g dbSNP:757523799
2038 2038 a, g dbSNP:754253874
2042 2042 g, t dbSNP:764451293
2045 2045 c, g dbSNP:188831675
2047 2047 a, c, g dbSNP:765978232
2052 2052 a, c dbSNP:150494360
2057 2057 c, t dbSNP:542139125
2062 2062 c, g dbSNP:753236884
2063 2063 c, t dbSNP:781775490
2066 2066 a, g dbSNP:757938445
2069 2069 a, g dbSNP:573618693
2084 2084 a, t dbSNP:764734893
2100 2100 a, c dbSNP:761527195
2106 2106 a, c dbSNP:199940967
2107 2107 a, g dbSNP:375501847
2122 2122 c, g dbSNP:760434383
2129 2129 a, g dbSNP:145033509
2133 2133 a, g dbSNP:184960717
2135 2135 a, g dbSNP:772021168
2165 2165 c, t dbSNP:369831560
2170 2170 a, c dbSNP:774419073
2193 2193 c, g dbSNP:557875371
2194 2194 c, t dbSNP:749516404
2206 2206 a, c dbSNP:547805228
2218 2218 a, t dbSNP:747505018
2225 2225 c, t dbSNP:140543776
2247 2247 c, t dbSNP:76693948
2248 2248 a, c, t dbSNP:80303950
2253 2253 c, t dbSNP:113746010
2271 2271 a, g dbSNP:370966099
2290 2290 a, g dbSNP:777427799
2295 2295 c, g dbSNP:755770851
2300 2300 c, t dbSNP:752414695
2304 2304 a, c dbSNP:192419029
2309 2309 c, g dbSNP:754820224
2316 2316 c, t dbSNP:751488260
2317 2317 a, g dbSNP:532641584
2322 2322 c, g, t dbSNP:768025111
2328 2328 a, g dbSNP:763043556
2330 2330 a, g dbSNP:181292654
2331 2331 a, t dbSNP:773175370
2338 2338 a, t dbSNP:765487058
2342 2342 c, t dbSNP:762118625
2343 2343 a, g dbSNP:777050595
2345 2345 a, g dbSNP:184968916
2348 2348 g, t dbSNP:769119901
2360 2360 a, g dbSNP:747417009
2366 2366 c, t dbSNP:776023975
2377 2377 a, g dbSNP:200195623
2379 2379 c, g dbSNP:570492580
2382 2382 a, g dbSNP:183923278
2383 2383 a, g dbSNP:188654478
2386 2386 a, g dbSNP:189948577
2393 2393 a, g dbSNP:780867284
2396 2396 c, g dbSNP:754730255
2402 2402 g, t dbSNP:751335821
2409 2409 a, g dbSNP:779980787
2411 2411 a, g dbSNP:758270070
2416 2416 g, t dbSNP:750436964
2418 2418 a, g dbSNP:765392455
2429 2429 a, t dbSNP:527800239
2431 2431 c, t dbSNP:185420432
2432 2432 a, g dbSNP:548502981
2448 2448 a, g dbSNP:112747048
2452 2452 a, g dbSNP:530305374
2522 2522 c, t dbSNP:759782658
2523 2523 a, g dbSNP:774726845
2542 2542 c, t dbSNP:563219537
2554 2554 a, g dbSNP:11550461
2557 2557 c, g dbSNP:759445626
2592 2592 a, g dbSNP:544896659
2634 2634 -, a, aa, aaa, aaaa dbSNP:59370839
2636 2636 -, aaa dbSNP:71852726
2653 2653 -, aaaaacaa, caaaaaaa dbSNP:575317929
2655 2655 -, aaa dbSNP:752834205
2656 2656 a, c dbSNP:808078
2656 2656 -, c dbSNP:373111906
2657 2657 -, aaa dbSNP:369756092
2657 2657 a, c dbSNP:71380964
2658 2658 a, c dbSNP:199765664
2661 2661 a, t dbSNP:1053393
2662 2662 -, aa dbSNP:369068093
2664 2664 c, t dbSNP:145606241
2718 2718 -, tttg dbSNP:564951006
2721 2721 a, g dbSNP:545755034
2729 2729 c, g dbSNP:572048744
2758 2758 a, g dbSNP:776500632
2775 2775 c, t dbSNP:543740178
2776 2776 a, g dbSNP:553790361
2797 2797 a, c dbSNP:181198454
2829 2829 c, t dbSNP:188743379
2876 2876 a, g dbSNP:774565598
2950 2950 a, t dbSNP:768759859
2958 2958 c, t dbSNP:542974709
2959 2959 g, t dbSNP:780922363
2962 2962 a, g dbSNP:754785560
2987 2987 c, t dbSNP:762727734
2992 2992 a, g dbSNP:746602580
3000 3000 a, t dbSNP:779756007
3019 3019 c, t dbSNP:749705519
3075 3075 a, t dbSNP:11550460
3114 3114 a, g dbSNP:758047233
3136 3136 -, t dbSNP:34926621
3137 3137 -, t dbSNP:544973710
3139 3139 c, t dbSNP:183646374
3189 3189 c, g dbSNP:575736800
3201 3201 c, t dbSNP:191441368
3219 3219 a, t dbSNP:1698957
3235 3235 a, g dbSNP:780263080
3256 3256 c, t dbSNP:560695968
3328 3328 c, t dbSNP:141839860
3360 3360 c, g dbSNP:542697419
3363 3363 c, g dbSNP:552312462
3414 3414 c, t dbSNP:794557
3456 3456 a, t dbSNP:79050797
3457 3457 c, t dbSNP:138252903
3480 3480 a, g dbSNP:145628294
3483 3483 c, t dbSNP:553910416
3484 3484 c, t dbSNP:112150674
3569 3569 c, g dbSNP:530343884
3592 3592 a, g dbSNP:115810403
3619 3619 c, t dbSNP:76259065
3621 3621 c, t dbSNP:148961435
3733 3733 a, g dbSNP:188470349
3755 3755 c, t dbSNP:545490118
3779 3779 g, t dbSNP:746409661
3853 3853 a, c dbSNP:539333964
3865 3865 -, t dbSNP:749537563
3894 3894 a, g dbSNP:571998815
3916 3916 a, g dbSNP:560037141
3923 3923 a, g dbSNP:794558
3933 3933 a, g dbSNP:774644453
3945 3945 c, g dbSNP:112333048
3960 3960 c, t dbSNP:555956898
3962 3962 a, t dbSNP:375977006
3974 3974 c, t dbSNP:763130659
3987 3987 -, c dbSNP:568752537
3991 3991 c, t dbSNP:371605094
4003 4003 c, g dbSNP:773307805
4019 4019 a, g dbSNP:537393708
4055 4055 c, t dbSNP:748025305
4060 4060 c, t dbSNP:11647138
4121 4121 a, c dbSNP:558779814
4159 4159 a, g dbSNP:768650421
4168 4168 a, g dbSNP:533970325
4213 4213 c, t dbSNP:566858054
4224 4224 a, c dbSNP:779764620
4258 4258 c, t dbSNP:794559
4298 4298 c, t dbSNP:374027606
4344 4344 a, c dbSNP:570059770
4412 4412 a, g dbSNP:548431200
4417 4417 c, t dbSNP:114654822
4428 4428 -, t dbSNP:200961009
4452 4452 a, g dbSNP:536731227
4464 4464 a, g dbSNP:752584142
4476 4476 g, t dbSNP:150247715
4477 4477 a, g dbSNP:112939943
4492 4492 g, t dbSNP:547249927
4496 4496 c, t dbSNP:527341985
4503 4503 c, t dbSNP:560084002
4542 4542 c, g dbSNP:753188016
4624 4624 a, g dbSNP:541382201
4637 4637 c, t dbSNP:184888157
4694 4694 a, g dbSNP:781763237
4705 4705 -, c dbSNP:780126752
4731 4731 -, a dbSNP:746904233
4829 4829 a, t dbSNP:755598539
4841 4841 c, t dbSNP:193290216

Target ORF information:

RefSeq Version XM_005256045
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005256045

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCCATTT CTGATGAACT GCACTACTTG GAGGTCTACC TGACAGATGA GTTTGCTAAA 
GGAAGGAAAG TGGCAGATCT CTACGAACTT GTACAGTATG CTGGAAACAT TATCCCAAGG
CTTTACCTTT TGATCACAGT TGGAGTTGTA TATGTCAAGT CATTTCCTCA GTCCAGGAAG
GATATTTTGA AAGATTTGGT AGAAATGTGC CGTGGTGTGC AACATCCCTT GAGGGGTCTG
TTTCTTCGAA ATTACCTTCT TCAGTGTACC AGAAATATCT TACCTGATGA AGGAGAGCCA
ACAGATGAAG AAACAACTGG TGACATCAGT GATTCCATGG ATTTTGTACT GCTCAACTTT
GCAGAAATGA ACAAGCTCTG GGTGCGAATG CAGCATCAGG GACATAGCCG AGATAGAGAA
AAAAGAGAAC GAGAAAGACA AGAACTGAGA ATTTTAGTGG GAACAAATTT GGTGCGCCTC
AGTCAGTTGG AAGGTGTAAA TGTGGAACGT TACAAACAGA TTGTTTTGAC TGGCATATTG
GAGCAAGTTG TAAACTGTAG GGATGCTTTG GCTCAAGAAT ATCTCATGGA GTGTATTATT
CAGGTTTTCC CTGATGAATT TCACCTCCAG ACTTTGAATC CTTTTCTTCG GGCCTGTGCT
GAGTTACACC AGAATGTAAA TGTGAAGAAC ATAATCATTG CTTTAATTGA TAGATTAGCT
TTATTTGCTC ACCGTGAAGA TGGACCTGGA ATCCCAGCGG ATATTAAACT TTTTGATATA
TTTTCACAGC AGGTGGCTAC AGTGATACAG TCTAGACAAG ACATGCCTTC AGAGGATGTT
GTATCTTTAC AAGTCTCTCT GATTAATCTT GCCATGAAAT GTTACCCTGA TCGTGTGGAC
TATGTTGATA AAGTTCTAGA AACAACAGTG GAGATATTCA ATAAGCTCAA CCTTGAACAT
ATTGCTACCA GTAGTGCAGT TTCAAAGGAA CTCACCAGAC TTTTGAAAAT ACCAGTTGAC
ACTTACAACA ATATTTTAAC AGTCTTGAAA TTAAAACATT TTCACCCACT CTTTGAGTAC
TTTGACTACG AGTCCAGAAA GAGCATGAGT TGTTATGTGC TTAGTAATGT TCTGGATTAT
AACACAGAAA TTGTCTCTCA AGACCAGGTG GATTCCATAA TGAATTTGGT ATCCACGTTG
ATTCAAGATC AGCCAGATCA ACCTGTAGAA GACCCTGATC CAGAAGATTT TGCTGATGAG
CAGAGCCTTG TGGGCCGCTT CATTCATCTG CTGCGCTCTG AGGACCCTGA CCAGCAGTAC
TTGATTTTGA ACACAGCACG AAAACATTTT GGAGCTGGTG GAAATCAGCG GATTCGCTTC
ACACTGCCAC CTTTGGTATT TGCAGCTTAC CAGCTGGCTT TTCGATATAA AGAGAATTCT
AAAGTGGATG ACAAATGGGA AAAGAAATGC CAGAAGATTT TTTCATTTGC CCACCAGACT
ATCAGTGCTT TGATCAAAGC AGAGCTGGCA GAATTGCCCT TAAGACTTTT TCTTCAAGGA
GCACTAGCTG CTGGGGAAAT TGGTTTTGAA AATCATGAGA CAGTCGCATA TGAATTCATG
TCCCAGGCAT TTTCTCTGTA TGAAGATGAA ATCAGCGATT CCAAAGCACA GCTAGCTGCC
ATCACCTTGA TCATTGGCAC TTTTGAAAGG ATGAAGTGCT TCAGTGAAGA GAATCACGAA
CCTCTGAGGA CTCAGTGTGC CCTTGCTGCA TCCAAACTTC TAAAGAAACC TGATCAGGGC
CGAGCTGTGA GCACCTGTGC ACATCTCTTC TGGTCTGGCA GAAACACGGA CAAAAATGGG
GAGGAGCTTC ACGGAGGCAA GAGGGTAATG GAGTGCCTAA AAAAAGCTCT AAAAATAGCA
AATCAGTGCA TGGACCCCTC TCTACAAGTG CAGCTTTTTA TAGAAATTCT GAACAGATAT
ATCTATTTTT ATGAAAAGGA AAATGATGCG GTAACAATTC AGGTTTTAAA CCAGCTTATC
CAAAAGATTC GAGAAGACCT CCCGAATCTT GAATCCAGTG AAGAAACAGA GCAGATTAAC
AAACATTTTC ATAACACACT GGAGCATTTG CGCTTGCGGC GGGAATCACC AGAATCCGAG
GGGCCAATTT ATGAAGGTCT CATCCTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18510
Accession Version NM_018206.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Homo sapiens (human)
Product vacuolar protein sorting-associated protein 35
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB049686.1, BC093036.1, BQ574179.1 and BM971213.1. This sequence is a reference standard in the RefSeqGene project. On Aug 29, 2007 this sequence version replaced gi:41352714. Summary: This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC093036.1, AF175265.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGCCTACAA CACAGCAGTC CCCTCAGGAT GAGCAGGAAA AGCTCTTGGA TGAAGCCATA 
CAGGCTGTGA AGGTCCAGTC ATTCCAAATG AAGAGATGCC TGGACAAAAA CAAGCTTATG
GATGCTCTAA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA
AAGAGTTACT ATGAACTTTA TATGGCCATT TCTGATGAAC TGCACTACTT GGAGGTCTAC
CTGACAGATG AGTTTGCTAA AGGAAGGAAA GTGGCAGATC TCTACGAACT TGTACAGTAT
GCTGGAAACA TTATCCCAAG GCTTTACCTT TTGATCACAG TTGGAGTTGT ATATGTCAAG
TCATTTCCTC AGTCCAGGAA GGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG
CAACATCCCT TGAGGGGTCT GTTTCTTCGA AATTACCTTC TTCAGTGTAC CAGAAATATC
TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGACATCAG TGATTCCATG
GATTTTGTAC TGCTCAACTT TGCAGAAATG AACAAGCTCT GGGTGCGAAT GCAGCATCAG
GGACATAGCC GAGATAGAGA AAAAAGAGAA CGAGAAAGAC AAGAACTGAG AATTTTAGTG
GGAACAAATT TGGTGCGCCT CAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG
ATTGTTTTGA CTGGCATATT GGAGCAAGTT GTAAACTGTA GGGATGCTTT GGCTCAAGAA
TATCTCATGG AGTGTATTAT TCAGGTTTTC CCTGATGAAT TTCACCTCCA GACTTTGAAT
CCTTTTCTTC GGGCCTGTGC TGAGTTACAC CAGAATGTAA ATGTGAAGAA CATAATCATT
GCTTTAATTG ATAGATTAGC TTTATTTGCT CACCGTGAAG ATGGACCTGG AATCCCAGCG
GATATTAAAC TTTTTGATAT ATTTTCACAG CAGGTGGCTA CAGTGATACA GTCTAGACAA
GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TGATTAATCT TGCCATGAAA
TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC
AATAAGCTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA
CTTTTGAAAA TACCAGTTGA CACTTACAAC AATATTTTAA CAGTCTTGAA ATTAAAACAT
TTTCACCCAC TCTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG TTGTTATGTG
CTTAGTAATG TTCTGGATTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA
ATGAATTTGG TATCCACGTT GATTCAAGAT CAGCCAGATC AACCTGTAGA AGACCCTGAT
CCAGAAGATT TTGCTGATGA GCAGAGCCTT GTGGGCCGCT TCATTCATCT GCTGCGCTCT
GAGGACCCTG ACCAGCAGTA CTTGATTTTG AACACAGCAC GAAAACATTT TGGAGCTGGT
GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT
TTTCGATATA AAGAGAATTC TAAAGTGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT
TTTTCATTTG CCCACCAGAC TATCAGTGCT TTGATCAAAG CAGAGCTGGC AGAATTGCCC
TTAAGACTTT TTCTTCAAGG AGCACTAGCT GCTGGGGAAA TTGGTTTTGA AAATCATGAG
ACAGTCGCAT ATGAATTCAT GTCCCAGGCA TTTTCTCTGT ATGAAGATGA AATCAGCGAT
TCCAAAGCAC AGCTAGCTGC CATCACCTTG ATCATTGGCA CTTTTGAAAG GATGAAGTGC
TTCAGTGAAG AGAATCATGA ACCTCTGAGG ACTCAGTGTG CCCTTGCTGC ATCCAAACTT
CTAAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CACATCTCTT CTGGTCTGGC
AGAAACACGG ACAAAAATGG GGAGGAGCTT CACGGAGGCA AGAGGGTAAT GGAGTGCCTA
AAAAAAGCTC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT GCAGCTTTTT
ATAGAAATTC TGAACAGATA TATCTATTTT TATGAAAAGG AAAATGATGC GGTAACAATT
CAGGTTTTAA ACCAGCTTAT CCAAAAGATT CGAGAAGACC TCCCGAATCT TGAATCCAGT
GAAGAAACAG AGCAGATTAA CAAACATTTT CATAACACAC TGGAGCATTT GCGCTTGCGG
CGGGAATCAC CAGAATCCGA GGGGCCAATT TATGAAGGTC TCATCCTTTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_060676.2
CDS100..2490
Misc Feature(1)40..42(+)
Misc Feature(2)118..120(+)
Misc Feature(3)139..2361(+)
Misc Feature(4)172..231(+)
Misc Feature(5)712..744(+)
Misc Feature(6)1411..2487(+)
Misc Feature(7)1597..2178(+)
Misc Feature(8)2470..2472(+)
Exon (1)1..102
Gene:VPS35
Gene Synonym:
Exon (2)103..201
Gene:VPS35
Gene Synonym:
Exon (3)202..298
Gene:VPS35
Gene Synonym:
Exon (4)299..422
Gene:VPS35
Gene Synonym:
Exon (5)423..605
Gene:VPS35
Gene Synonym:
Exon (6)606..819
Gene:VPS35
Gene Synonym:
Exon (7)820..903
Gene:VPS35
Gene Synonym:
Exon (8)904..1013
Gene:VPS35
Gene Synonym:
Exon (9)1014..1110
Gene:VPS35
Gene Synonym:
Exon (10)1111..1259
Gene:VPS35
Gene Synonym:
Exon (11)1260..1467
Gene:VPS35
Gene Synonym:
Exon (12)1468..1623
Gene:VPS35
Gene Synonym:
Exon (13)1624..1746
Gene:VPS35
Gene Synonym:
Exon (14)1747..1926
Gene:VPS35
Gene Synonym:
Exon (15)1927..2166
Gene:VPS35
Gene Synonym:
Exon (16)2167..2310
Gene:VPS35
Gene Synonym:
Exon (17)2311..3285
Gene:VPS35
Gene Synonym:
Translation
Position Chain Variation Link
4 4 a, g dbSNP:531880412
12 12 c, t dbSNP:563324788
13 13 a, g dbSNP:551106108
48 48 a, g dbSNP:761175510
51 51 c, g dbSNP:773461813
55 55 a, g dbSNP:767874057
58 58 a, c dbSNP:762053892
59 59 a, g dbSNP:774526654
61 61 g, t dbSNP:768774857
64 64 c, g dbSNP:749358646
65 65 c, t dbSNP:398122321
65 65 -, t dbSNP:772743562
65 65 c, g, t dbSNP:189775907
66 66 a, c, g, t dbSNP:3743928
70 70 a, g dbSNP:746704070
71 71 c, t dbSNP:182309233
76 76 c, t dbSNP:541080284
77 77 a, g dbSNP:758046563
78 78 c, t dbSNP:752258031
81 81 a, c, t dbSNP:573920049
82 82 a, c, g dbSNP:767812765
84 84 c, g dbSNP:762144323
85 85 a, c dbSNP:774616523
87 87 c, t dbSNP:764298631
90 90 g, t dbSNP:763096994
91 91 c, g dbSNP:778763088
96 96 c, t dbSNP:775449983
106 106 a, g dbSNP:774604183
132 132 a, g dbSNP:771229656
145 145 c, t dbSNP:542307781
149 149 a, t dbSNP:773381942
150 150 c, t dbSNP:770188899
155 155 c, g dbSNP:370411054
159 159 a, g dbSNP:781646463
163 163 a, g dbSNP:769187894
178 178 g, t dbSNP:745338373
188 188 a, t dbSNP:145147781
209 209 a, c, g dbSNP:777006799
243 243 c, t dbSNP:769097906
250 250 a, g dbSNP:193077277
259 259 c, t dbSNP:186122975
260 260 a, g dbSNP:770464667
267 267 c, t dbSNP:748800470
268 268 a, g, t dbSNP:375285388
270 270 a, g dbSNP:183554824
293 293 c, t dbSNP:11550464
307 307 a, t dbSNP:781057884
311 311 c, t dbSNP:754806601
323 323 a, g dbSNP:746848785
324 324 c, g dbSNP:529522862
326 326 a, g dbSNP:758294660
329 329 c, t dbSNP:11550462
344 344 c, g dbSNP:188245364
347 347 a, t dbSNP:12925313
348 348 a, t dbSNP:12920740
365 365 a, g dbSNP:750463506
384 384 c, t dbSNP:375122817
390 390 c, t dbSNP:757397775
414 414 a, c, g dbSNP:764351948
426 426 c, t dbSNP:768460283
438 438 a, g dbSNP:746758894
477 477 a, g, t dbSNP:141444850
481 481 a, g dbSNP:375986040
551 551 -, cag dbSNP:747313765
552 552 -, g dbSNP:780429873
556 556 -, agaaatatc dbSNP:758873324
565 565 c, t dbSNP:778970704
570 570 c, g dbSNP:757308043
580 580 c, t dbSNP:370380458
583 583 a, c dbSNP:777932822
591 591 a, g dbSNP:184863243
595 595 a, g dbSNP:756353898
599 599 a, c dbSNP:753047950
609 609 a, g dbSNP:770887209
636 636 a, c dbSNP:749320030
649 649 c, t dbSNP:373346821
662 662 a, c dbSNP:200388382
675 675 a, g dbSNP:756262411
685 685 c, t dbSNP:201098565
702 702 a, c dbSNP:781534743
703 703 a, c dbSNP:79423190
708 708 c, t dbSNP:755273544
709 709 a, c dbSNP:369424393
714 714 c, t dbSNP:766949427
741 741 a, g dbSNP:758993631
744 744 a, g dbSNP:753336800
747 747 a, g dbSNP:763697798
756 756 a, g dbSNP:760294326
777 777 c, t dbSNP:376845827
778 778 c, t dbSNP:142925902
782 782 c, g dbSNP:201996601
790 790 -, g dbSNP:35060795
813 813 c, t dbSNP:759216006
822 822 g, t dbSNP:192783364
847 847 a, g dbSNP:375809380
861 861 a, g dbSNP:766158776
885 885 c, t dbSNP:762793303
895 895 a, g dbSNP:773110975
908 908 g, t dbSNP:761821222
909 909 c, t dbSNP:776505532
918 918 a, g dbSNP:768773395
929 929 a, g dbSNP:760891909
933 933 c, t dbSNP:148572750
940 940 c, g dbSNP:772459263
948 948 g, t dbSNP:746190765
949 949 c, t dbSNP:779434158
950 950 a, g dbSNP:771276024
963 963 a, g dbSNP:749799639
964 964 g, t dbSNP:778203456
967 967 c, t dbSNP:754487190
974 974 a, g dbSNP:751107774
982 982 g, t dbSNP:779641969
991 991 a, g dbSNP:758068523
1011 1011 c, t dbSNP:750157630
1013 1013 a, g dbSNP:371218680
1014 1014 a, t dbSNP:77133510
1017 1017 a, t dbSNP:756137934
1029 1029 c, t dbSNP:752758821
1033 1033 c, t dbSNP:767701361
1034 1034 a, g dbSNP:759749769
1038 1038 a, g dbSNP:200578690
1042 1042 a, g dbSNP:774611405
1044 1044 a, t dbSNP:144346159
1045 1045 c, t dbSNP:770029606
1046 1046 c, g, t dbSNP:770226984
1047 1047 c, t dbSNP:748655024
1054 1054 c, t dbSNP:777184680
1058 1058 c, t dbSNP:747944333
1059 1059 a, g dbSNP:745416544
1061 1061 a, g dbSNP:778590455
1062 1062 c, t dbSNP:756884549
1065 1065 g, t dbSNP:749058909
1072 1072 c, t dbSNP:777437956
1077 1077 c, t dbSNP:756040992
1078 1078 a, g dbSNP:752668855
1087 1087 c, t dbSNP:767613694
1119 1119 a, g dbSNP:188883365
1134 1134 a, g dbSNP:563975281
1141 1141 a, g dbSNP:140265194
1155 1155 c, t dbSNP:769692526
1158 1158 c, t dbSNP:747997807
1174 1174 a, g dbSNP:781234198
1179 1179 a, g dbSNP:754892097
1180 1180 g, t dbSNP:751599316
1186 1186 c, t dbSNP:780001713
1192 1192 c, t dbSNP:758568605
1193 1193 a, g dbSNP:201593831
1209 1209 c, t dbSNP:113154062
1218 1218 a, g dbSNP:765489808
1223 1223 a, c dbSNP:762131827
1237 1237 c, t dbSNP:754226798
1241 1241 a, g, t dbSNP:754978683
1244 1244 a, g dbSNP:116254156
1247 1247 c, t dbSNP:770335442
1250 1250 a, g dbSNP:762573165
1251 1251 c, t dbSNP:772970332
1269 1269 c, t dbSNP:757485744
1275 1275 -, agt dbSNP:779224141
1279 1279 a, g dbSNP:754137147
1294 1294 a, g dbSNP:764365507
1328 1328 a, g dbSNP:756559738
1354 1354 a, t dbSNP:370055356
1361 1361 a, t dbSNP:375087873
1363 1363 c, t dbSNP:762332686
1367 1367 c, t dbSNP:373075116
1376 1376 a, c dbSNP:764924463
1383 1383 c, t dbSNP:530665086
1389 1389 c, t dbSNP:368876504
1390 1390 a, g, t dbSNP:143688112
1391 1391 a, t dbSNP:139200616
1396 1396 a, c dbSNP:191165923
1412 1412 a, g dbSNP:745893720
1416 1416 c, t dbSNP:187425753
1426 1426 a, g dbSNP:376209552
1432 1432 c, g dbSNP:749503515
1439 1439 a, t dbSNP:778105954
1443 1443 c, t dbSNP:756404549
1452 1452 c, t dbSNP:753137936
1457 1457 c, t dbSNP:17850640
1461 1461 a, g dbSNP:767864451
1477 1477 a, g dbSNP:367611000
1493 1493 c, g dbSNP:748396803
1496 1496 c, t dbSNP:781327081
1497 1497 a, g dbSNP:150810185
1502 1502 c, t dbSNP:747414987
1506 1506 a, g dbSNP:780663505
1515 1515 a, t dbSNP:374074473
1519 1519 c, g dbSNP:370401767
1525 1525 g, t dbSNP:376308396
1526 1526 c, t dbSNP:373113084
1527 1527 a, g dbSNP:528116722
1533 1533 c, g dbSNP:767202087
1534 1534 a, c dbSNP:759288056
1546 1546 g, t dbSNP:751379541
1567 1567 c, t dbSNP:567251715
1570 1570 a, g dbSNP:766248974
1572 1572 a, g dbSNP:762949831
1574 1574 a, g dbSNP:747880925
1575 1575 c, t dbSNP:773089248
1579 1579 a, t dbSNP:141253289
1582 1582 a, t dbSNP:761848190
1587 1587 c, t dbSNP:776877370
1593 1593 a, g dbSNP:769027812
1594 1594 c, t dbSNP:747325352
1595 1595 a, g dbSNP:780577406
1596 1596 c, g dbSNP:772634549
1602 1602 a, g dbSNP:267604550
1603 1603 g, t dbSNP:746375559
1615 1615 a, c dbSNP:779340678
1616 1616 a, t dbSNP:755622603
1632 1632 c, t dbSNP:373955385
1634 1634 c, t dbSNP:757154329
1669 1669 c, t dbSNP:184277092
1674 1674 a, t dbSNP:753900468
1675 1675 c, t dbSNP:398124658
1685 1685 a, t dbSNP:760887864
1689 1689 a, g dbSNP:775824787
1690 1690 c, t dbSNP:369196817
1701 1701 c, t dbSNP:376718984
1713 1713 g, t dbSNP:759900117
1735 1735 a, c dbSNP:774595498
1740 1740 c, t dbSNP:771405301
1741 1741 a, g dbSNP:749793948
1744 1744 c, g dbSNP:568160306
1747 1747 c, g dbSNP:748389084
1750 1750 a, g dbSNP:779063544
1752 1752 c, t dbSNP:533283266
1768 1768 a, t dbSNP:13332463
1776 1776 g, t dbSNP:770312643
1778 1778 c, t dbSNP:760128592
1801 1801 a, g dbSNP:774875351
1802 1802 c, t dbSNP:376786968
1840 1840 c, t dbSNP:745500235
1845 1845 a, g dbSNP:778678166
1875 1875 c, g dbSNP:770819201
1878 1878 a, t dbSNP:749127069
1882 1882 a, c, g dbSNP:201566773
1894 1894 c, g dbSNP:148255605
1899 1899 a, g dbSNP:752760936
1902 1902 a, g dbSNP:145679337
1904 1904 a, t dbSNP:34687100
1905 1905 c, t dbSNP:755145722
1908 1908 a, g, t dbSNP:142063738
1956 1956 c, t dbSNP:758632241
1957 1957 a, g dbSNP:188286943
1976 1976 c, t dbSNP:201998902
1980 1980 c, t dbSNP:138794859
1982 1982 c, t dbSNP:757674131
1990 1990 a, c dbSNP:754270736
1993 1993 a, g dbSNP:764695026
1994 1994 c, t dbSNP:756550058
2000 2000 c, g dbSNP:758922774
2004 2004 g, t dbSNP:569735490
2011 2011 a, t dbSNP:144997996
2037 2037 c, t dbSNP:168745
2044 2044 c, t dbSNP:145406665
2055 2055 a, g dbSNP:769692699
2070 2070 a, g dbSNP:748012697
2073 2073 c, t dbSNP:559637051
2075 2075 a, g dbSNP:776389848
2106 2106 c, t dbSNP:376700700
2115 2115 a, c dbSNP:746999621
2119 2119 g, t dbSNP:780111914
2121 2121 a, g dbSNP:375643041
2133 2133 g, t dbSNP:758539394
2134 2134 c, t dbSNP:547369855
2146 2146 a, g dbSNP:779153929
2148 2148 a, g dbSNP:757523799
2153 2153 a, g dbSNP:754253874
2157 2157 g, t dbSNP:764451293
2160 2160 c, g dbSNP:188831675
2162 2162 a, c, g dbSNP:765978232
2167 2167 a, c dbSNP:150494360
2172 2172 c, t dbSNP:542139125
2177 2177 c, g dbSNP:753236884
2178 2178 c, t dbSNP:781775490
2181 2181 a, g dbSNP:757938445
2184 2184 a, g dbSNP:573618693
2199 2199 a, t dbSNP:764734893
2215 2215 a, c dbSNP:761527195
2221 2221 a, c dbSNP:199940967
2222 2222 a, g dbSNP:375501847
2237 2237 c, g dbSNP:760434383
2244 2244 a, g dbSNP:145033509
2248 2248 a, g dbSNP:184960717
2250 2250 a, g dbSNP:772021168
2280 2280 c, t dbSNP:369831560
2285 2285 a, c dbSNP:774419073
2308 2308 c, g dbSNP:557875371
2309 2309 c, t dbSNP:749516404
2321 2321 a, c dbSNP:547805228
2333 2333 a, t dbSNP:747505018
2340 2340 c, t dbSNP:140543776
2362 2362 c, t dbSNP:76693948
2363 2363 a, c, t dbSNP:80303950
2368 2368 c, t dbSNP:113746010
2386 2386 a, g dbSNP:370966099
2405 2405 a, g dbSNP:777427799
2410 2410 c, g dbSNP:755770851
2415 2415 c, t dbSNP:752414695
2419 2419 a, c dbSNP:192419029
2424 2424 c, g dbSNP:754820224
2431 2431 c, t dbSNP:751488260
2432 2432 a, g dbSNP:532641584
2437 2437 c, g, t dbSNP:768025111
2443 2443 a, g dbSNP:763043556
2445 2445 a, g dbSNP:181292654
2446 2446 a, t dbSNP:773175370
2453 2453 a, t dbSNP:765487058
2457 2457 c, t dbSNP:762118625
2458 2458 a, g dbSNP:777050595
2460 2460 a, g dbSNP:184968916
2463 2463 g, t dbSNP:769119901
2475 2475 a, g dbSNP:747417009
2481 2481 c, t dbSNP:776023975
2492 2492 a, g dbSNP:200195623
2494 2494 c, g dbSNP:570492580
2497 2497 a, g dbSNP:183923278
2498 2498 a, g dbSNP:188654478
2501 2501 a, g dbSNP:189948577
2508 2508 a, g dbSNP:780867284
2511 2511 c, g dbSNP:754730255
2517 2517 g, t dbSNP:751335821
2524 2524 a, g dbSNP:779980787
2526 2526 a, g dbSNP:758270070
2531 2531 g, t dbSNP:750436964
2533 2533 a, g dbSNP:765392455
2544 2544 a, t dbSNP:527800239
2546 2546 c, t dbSNP:185420432
2547 2547 a, g dbSNP:548502981
2563 2563 a, g dbSNP:112747048
2567 2567 a, g dbSNP:530305374
2637 2637 c, t dbSNP:759782658
2638 2638 a, g dbSNP:774726845
2657 2657 c, t dbSNP:563219537
2669 2669 a, g dbSNP:11550461
2672 2672 c, g dbSNP:759445626
2707 2707 a, g dbSNP:544896659
2749 2749 -, a, aa, aaa, aaaa dbSNP:59370839
2751 2751 -, aaa dbSNP:71852726
2768 2768 -, aaaaacaa, caaaaaaa dbSNP:575317929
2770 2770 -, aaa dbSNP:752834205
2771 2771 a, c dbSNP:808078
2771 2771 -, c dbSNP:373111906
2772 2772 -, aaa dbSNP:369756092
2772 2772 a, c dbSNP:71380964
2773 2773 a, c dbSNP:199765664
2776 2776 a, t dbSNP:1053393
2777 2777 -, aa dbSNP:369068093
2779 2779 c, t dbSNP:145606241
2833 2833 -, tttg dbSNP:564951006
2836 2836 a, g dbSNP:545755034
2844 2844 c, g dbSNP:572048744
2873 2873 a, g dbSNP:776500632
2890 2890 c, t dbSNP:543740178
2891 2891 a, g dbSNP:553790361
2912 2912 a, c dbSNP:181198454
2944 2944 c, t dbSNP:188743379
2991 2991 a, g dbSNP:774565598
3065 3065 a, t dbSNP:768759859
3073 3073 c, t dbSNP:542974709
3074 3074 g, t dbSNP:780922363
3077 3077 a, g dbSNP:754785560
3102 3102 c, t dbSNP:762727734
3107 3107 a, g dbSNP:746602580
3115 3115 a, t dbSNP:779756007
3134 3134 c, t dbSNP:749705519
3190 3190 a, t dbSNP:11550460
3229 3229 a, g dbSNP:758047233
3251 3251 -, t dbSNP:34926621
3252 3252 -, t dbSNP:544973710
3254 3254 c, t dbSNP:183646374

Target ORF information:

RefSeq Version NM_018206
Organism Homo sapiens (human)
Definition Homo sapiens VPS35 retromer complex component (VPS35), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_018206

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGCCTACAA CACAGCAGTC CCCTCAGGAT GAGCAGGAAA AGCTCTTGGA TGAAGCCATA 
CAGGCTGTGA AGGTCCAGTC ATTCCAAATG AAGAGATGCC TGGACAAAAA CAAGCTTATG
GATGCTCTAA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA
AAGAGTTACT ATGAACTTTA TATGGCCATT TCTGATGAAC TGCACTACTT GGAGGTCTAC
CTGACAGATG AGTTTGCTAA AGGAAGGAAA GTGGCAGATC TCTACGAACT TGTACAGTAT
GCTGGAAACA TTATCCCAAG GCTTTACCTT TTGATCACAG TTGGAGTTGT ATATGTCAAG
TCATTTCCTC AGTCCAGGAA GGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG
CAACATCCCT TGAGGGGTCT GTTTCTTCGA AATTACCTTC TTCAGTGTAC CAGAAATATC
TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGACATCAG TGATTCCATG
GATTTTGTAC TGCTCAACTT TGCAGAAATG AACAAGCTCT GGGTGCGAAT GCAGCATCAG
GGACATAGCC GAGATAGAGA AAAAAGAGAA CGAGAAAGAC AAGAACTGAG AATTTTAGTG
GGAACAAATT TGGTGCGCCT CAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG
ATTGTTTTGA CTGGCATATT GGAGCAAGTT GTAAACTGTA GGGATGCTTT GGCTCAAGAA
TATCTCATGG AGTGTATTAT TCAGGTTTTC CCTGATGAAT TTCACCTCCA GACTTTGAAT
CCTTTTCTTC GGGCCTGTGC TGAGTTACAC CAGAATGTAA ATGTGAAGAA CATAATCATT
GCTTTAATTG ATAGATTAGC TTTATTTGCT CACCGTGAAG ATGGACCTGG AATCCCAGCG
GATATTAAAC TTTTTGATAT ATTTTCACAG CAGGTGGCTA CAGTGATACA GTCTAGACAA
GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TGATTAATCT TGCCATGAAA
TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC
AATAAGCTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA
CTTTTGAAAA TACCAGTTGA CACTTACAAC AATATTTTAA CAGTCTTGAA ATTAAAACAT
TTTCACCCAC TCTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG TTGTTATGTG
CTTAGTAATG TTCTGGATTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA
ATGAATTTGG TATCCACGTT GATTCAAGAT CAGCCAGATC AACCTGTAGA AGACCCTGAT
CCAGAAGATT TTGCTGATGA GCAGAGCCTT GTGGGCCGCT TCATTCATCT GCTGCGCTCT
GAGGACCCTG ACCAGCAGTA CTTGATTTTG AACACAGCAC GAAAACATTT TGGAGCTGGT
GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT
TTTCGATATA AAGAGAATTC TAAAGTGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT
TTTTCATTTG CCCACCAGAC TATCAGTGCT TTGATCAAAG CAGAGCTGGC AGAATTGCCC
TTAAGACTTT TTCTTCAAGG AGCACTAGCT GCTGGGGAAA TTGGTTTTGA AAATCATGAG
ACAGTCGCAT ATGAATTCAT GTCCCAGGCA TTTTCTCTGT ATGAAGATGA AATCAGCGAT
TCCAAAGCAC AGCTAGCTGC CATCACCTTG ATCATTGGCA CTTTTGAAAG GATGAAGTGC
TTCAGTGAAG AGAATCATGA ACCTCTGAGG ACTCAGTGTG CCCTTGCTGC ATCCAAACTT
CTAAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CACATCTCTT CTGGTCTGGC
AGAAACACGG ACAAAAATGG GGAGGAGCTT CACGGAGGCA AGAGGGTAAT GGAGTGCCTA
AAAAAAGCTC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT GCAGCTTTTT
ATAGAAATTC TGAACAGATA TATCTATTTT TATGAAAAGG AAAATGATGC GGTAACAATT
CAGGTTTTAA ACCAGCTTAT CCAAAAGATT CGAGAAGACC TCCCGAATCT TGAATCCAGT
GAAGAAACAG AGCAGATTAA CAAACATTTT CATAACACAC TGGAGCATTT GCGCTTGCGG
CGGGAATCAC CAGAATCCGA GGGGCCAATT TATGAAGGTC TCATCCTTTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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