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PRKCB cDNA ORF clone, Homo sapiens (human)

Gene Symbol PRKCB
Entrez Gene ID 5579
Full Name protein kinase C, beta
Synonyms PKC-beta, PKCB, PRKCB1, PRKCB2
General protein information
Preferred Names
protein kinase C beta type
Names
protein kinase C beta type
PKC-B
protein kinase C, beta 1 polypeptide
NP_002729.2
NP_997700.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

16

16p11.2

Summary Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

176970


mRNA and Protein(s)

mRNA Protein Name
NM_002738 NP_002729 protein kinase C beta type isoform 2
NM_212535 NP_997700 protein kinase C beta type isoform 1



Homo sapiens (human) PRKCB NP_002729.2
Pan troglodytes (chimpanzee) PRKCB XP_003315067.1
Macaca mulatta (Rhesus monkey) PRKCB XP_001096447.1
Canis lupus familiaris (dog) PRKCB XP_005622125.1
Bos taurus (cattle) PRKCB NP_777012.1
Mus musculus (house mouse) Prkcb NP_032881.1
Rattus norvegicus (Norway rat) Prkcb NP_036845.3
Gallus gallus (chicken) PRKCB XP_414868.4
Danio rerio (zebrafish) prkcbb NP_957272.1
Xenopus (Silurana) tropicalis (western clawed frog) prkcb NP_001103513.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PRKCB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKCB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18081
NM_002738 Homo sapiens protein kinase C, beta (PRKCB), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu17455
NM_212535 Homo sapiens protein kinase C, beta (PRKCB), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$339.50-$475.30
$679.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18081
Clone ID Related Accession (Same CDS sequence) NM_002738
Accession Version NM_002738.6 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2022bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product protein kinase C beta type isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB456157.1, BC036472.1 and AC130448.2. This sequence is a reference standard in the RefSeqGene project. On Aug 20, 2008 this sequence version replaced gi:47157320. Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X07109.1, BC036472.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGCGAGGGCG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCATGAGG TCAAGAACCA CAAATTCACC
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC
GGGAAGCAGG GATTCCAGTG CCAAGTTTGC TGCTTTGTGG TGCACAAGCG GTGCCATGAA
TTTGTCACAT TCTCCTGCCC TGGCGCTGAC AAGGGTCCAG CCTCCGATGA CCCCCGCAGC
AAACACAAGT TTAAGATCCA CACGTACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCA
CTGCTGTATG GACTCATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT GAATGTGCAC
AAGCGCTGCG TGATGAATGT TCCCAGCCTG TGTGGCACGG ACCACACGGA GCGCCGCGGC
CGCATCTACA TCCAGGCCCA CATCGACAGG GACGTCCTCA TTGTCCTCGT AAGAGATGCT
AAAAACCTTG TACCTATGGA CCCCAATGGC CTGTCAGATC CCTACGTAAA ACTGAAACTG
ATTCCCGATC CCAAAAGTGA GAGCAAACAG AAGACCAAAA CCATCAAATG CTCCCTCAAC
CCTGAGTGGA ATGAGACATT TAGATTTCAG CTGAAAGAAT CGGACAAAGA CAGAAGACTG
TCAGTAGAGA TTTGGGATTG GGATTTGACC AGCAGGAATG ACTTCATGGG ATCTTTGTCC
TTTGGGATTT CTGAACTTCA GAAAGCCAGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG
GAGGAAGGCG AGTACTTCAA TGTGCCTGTG CCACCAGAAG GAAGTGAGGC CAATGAAGAA
CTGCGGCAGA AATTTGAGAG GGCCAAGATC AGTCAGGGAA CCAAGGTCCC GGAAGAAAAG
ACGACCAACA CTGTCTCCAA ATTTGACAAC AATGGCAACA GAGACCGGAT GAAACTGACC
GATTTTAACT TCCTAATGGT GCTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTTTCAGAA
CGAAAAGGCA CAGATGAGCT CTATGCTGTG AAGATCCTGA AGAAGGACGT TGTGATCCAA
GATGATGACG TGGAGTGCAC TATGGTGGAG AAGCGGGTGT TGGCCCTGCC TGGGAAGCCG
CCCTTCCTGA CCCAGCTCCA CTCCTGCTTC CAGACCATGG ACCGCCTGTA CTTTGTGATG
GAGTACGTGA ATGGGGGCGA CCTCATGTAT CACATCCAGC AAGTCGGCCG GTTCAAGGAG
CCCCATGCTG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT ACAGAGTAAG
GGCATCATTT ACCGTGACCT AAAACTTGAC AACGTGATGC TCGATTCTGA GGGACACATC
AAGATTGCCG ATTTTGGCAT GTGTAAGGAA AACATCTGGG ATGGGGTGAC AACCAAGACA
TTCTGTGGCA CTCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC CTATGGGAAG
TCCGTGGATT GGTGGGCATT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG GCAGGCACCC
TTTGAAGGGG AGGATGAAGA TGAACTCTTC CAATCCATCA TGGAACACAA CGTAGCCTAT
CCCAAGTCTA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATGAC CAAACACCCA
GGCAAACGTC TGGGTTGTGG ACCTGAAGGC GAACGTGATA TCAAAGAGCA TGCATTTTTC
CGGTATATTG ATTGGGAGAA ACTTGAACGC AAAGAGATCC AGCCCCCTTA TAAGCCAAAA
GCTTGTGGGC GAAATGCTGA AAACTTCGAC CGATTTTTCA CCCGCCATCC ACCAGTCCTA
ACACCTCCCG ACCAGGAAGT CATCAGGAAT ATTGACCAAT CAGAATTCGA AGGATTTTCC
TTTGTTAACT CTGAATTTTT AAAACCCGAA GTCAAGAGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002729.2
CDS198..2219
Misc Feature(1)30..32(+)
Misc Feature(2)243..245(+)
Misc Feature(3)246..248(+)
Misc Feature(4)306..455(+)
Misc Feature(5)306..455(+)
Misc Feature(6)327..386(+)
Misc Feature(7)336..344(+)
Misc Feature(8)501..650(+)
Misc Feature(9)501..650(+)
Misc Feature(10)522..581(+)
Misc Feature(11)531..539(+)
Misc Feature(12)672..1064(+)
Misc Feature(13)756..959(+)
Misc Feature(14)1218..2186(+)
Misc Feature(15)1221..1997(+)
Misc Feature(16)1239..1823(+)
Misc Feature(17)1239..1649(+)
Misc Feature(18)1251..1823(+)
Misc Feature(19)1644..1715(+)
Misc Feature(20)1695..1697(+)
Misc Feature(21)2118..2120(+)
Misc Feature(22)2118..2120(+)
Misc Feature(23)2163..2180(+)
Exon (1)1..370
Gene:PRKCB
Gene Synonym:
Exon (2)371..402
Gene:PRKCB
Gene Synonym:
Exon (3)403..485
Gene:PRKCB
Gene Synonym:
Exon (4)486..597
Gene:PRKCB
Gene Synonym:
Exon (5)598..726
Gene:PRKCB
Gene Synonym:
Exon (6)727..883
Gene:PRKCB
Gene Synonym:
Exon (7)884..1018
Gene:PRKCB
Gene Synonym:
Exon (8)1019..1115
Gene:PRKCB
Gene Synonym:
Exon (9)1116..1262
Gene:PRKCB
Gene Synonym:
Exon (10)1263..1436
Gene:PRKCB
Gene Synonym:
Exon (11)1437..1528
Gene:PRKCB
Gene Synonym:
Exon (12)1529..1591
Gene:PRKCB
Gene Synonym:
Exon (13)1592..1730
Gene:PRKCB
Gene Synonym:
Exon (14)1731..1811
Gene:PRKCB
Gene Synonym:
Exon (15)1812..1919
Gene:PRKCB
Gene Synonym:
Exon (16)1920..2060
Gene:PRKCB
Gene Synonym:
Exon (17)2061..8014
Gene:PRKCB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002738
Organism Homo sapiens (human)
Definition Homo sapiens protein kinase C, beta (PRKCB), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002738

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGCGAGGGCG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCATGAGG TCAAGAACCA CAAATTCACC
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC
GGGAAGCAGG GATTCCAGTG CCAAGTTTGC TGCTTTGTGG TGCACAAGCG GTGCCATGAA
TTTGTCACAT TCTCCTGCCC TGGCGCTGAC AAGGGTCCAG CCTCCGATGA CCCCCGCAGC
AAACACAAGT TTAAGATCCA CACGTACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCA
CTGCTGTATG GACTCATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT GAATGTGCAC
AAGCGCTGCG TGATGAATGT TCCCAGCCTG TGTGGCACGG ACCACACGGA GCGCCGCGGC
CGCATCTACA TCCAGGCCCA CATCGACAGG GACGTCCTCA TTGTCCTCGT AAGAGATGCT
AAAAACCTTG TACCTATGGA CCCCAATGGC CTGTCAGATC CCTACGTAAA ACTGAAACTG
ATTCCCGATC CCAAAAGTGA GAGCAAACAG AAGACCAAAA CCATCAAATG CTCCCTCAAC
CCTGAGTGGA ATGAGACATT TAGATTTCAG CTGAAAGAAT CGGACAAAGA CAGAAGACTG
TCAGTAGAGA TTTGGGATTG GGATTTGACC AGCAGGAATG ACTTCATGGG ATCTTTGTCC
TTTGGGATTT CTGAACTTCA GAAAGCCAGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG
GAGGAAGGCG AGTACTTCAA TGTGCCTGTG CCACCAGAAG GAAGTGAGGC CAATGAAGAA
CTGCGGCAGA AATTTGAGAG GGCCAAGATC AGTCAGGGAA CCAAGGTCCC GGAAGAAAAG
ACGACCAACA CTGTCTCCAA ATTTGACAAC AATGGCAACA GAGACCGGAT GAAACTGACC
GATTTTAACT TCCTAATGGT GCTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTTTCAGAA
CGAAAAGGCA CAGATGAGCT CTATGCTGTG AAGATCCTGA AGAAGGACGT TGTGATCCAA
GATGATGACG TGGAGTGCAC TATGGTGGAG AAGCGGGTGT TGGCCCTGCC TGGGAAGCCG
CCCTTCCTGA CCCAGCTCCA CTCCTGCTTC CAGACCATGG ACCGCCTGTA CTTTGTGATG
GAGTACGTGA ATGGGGGCGA CCTCATGTAT CACATCCAGC AAGTCGGCCG GTTCAAGGAG
CCCCATGCTG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT ACAGAGTAAG
GGCATCATTT ACCGTGACCT AAAACTTGAC AACGTGATGC TCGATTCTGA GGGACACATC
AAGATTGCCG ATTTTGGCAT GTGTAAGGAA AACATCTGGG ATGGGGTGAC AACCAAGACA
TTCTGTGGCA CTCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC CTATGGGAAG
TCCGTGGATT GGTGGGCATT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG GCAGGCACCC
TTTGAAGGGG AGGATGAAGA TGAACTCTTC CAATCCATCA TGGAACACAA CGTAGCCTAT
CCCAAGTCTA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATGAC CAAACACCCA
GGCAAACGTC TGGGTTGTGG ACCTGAAGGC GAACGTGATA TCAAAGAGCA TGCATTTTTC
CGGTATATTG ATTGGGAGAA ACTTGAACGC AAAGAGATCC AGCCCCCTTA TAAGCCAAAA
GCTTGTGGGC GAAATGCTGA AAACTTCGAC CGATTTTTCA CCCGCCATCC ACCAGTCCTA
ACACCTCCCG ACCAGGAAGT CATCAGGAAT ATTGACCAAT CAGAATTCGA AGGATTTTCC
TTTGTTAACT CTGAATTTTT AAAACCCGAA GTCAAGAGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17455
Clone ID Related Accession (Same CDS sequence) NM_212535
Accession Version NM_212535.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2016bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product protein kinase C beta type isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB456157.1, X06318.1, BC036472.1 and AC130448.2. On Aug 20, 2008 this sequence version replaced gi:47157321. Transcript Variant: This variant (1) uses an alternate splice junction at the 5' end of the last exon compared to variant 2. The resulting isoform (1) has a distinct and shorter C-terminus compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X06318.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGCGAGGGCG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCATGAGG TCAAGAACCA CAAATTCACC
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC
GGGAAGCAGG GATTCCAGTG CCAAGTTTGC TGCTTTGTGG TGCACAAGCG GTGCCATGAA
TTTGTCACAT TCTCCTGCCC TGGCGCTGAC AAGGGTCCAG CCTCCGATGA CCCCCGCAGC
AAACACAAGT TTAAGATCCA CACGTACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCA
CTGCTGTATG GACTCATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT GAATGTGCAC
AAGCGCTGCG TGATGAATGT TCCCAGCCTG TGTGGCACGG ACCACACGGA GCGCCGCGGC
CGCATCTACA TCCAGGCCCA CATCGACAGG GACGTCCTCA TTGTCCTCGT AAGAGATGCT
AAAAACCTTG TACCTATGGA CCCCAATGGC CTGTCAGATC CCTACGTAAA ACTGAAACTG
ATTCCCGATC CCAAAAGTGA GAGCAAACAG AAGACCAAAA CCATCAAATG CTCCCTCAAC
CCTGAGTGGA ATGAGACATT TAGATTTCAG CTGAAAGAAT CGGACAAAGA CAGAAGACTG
TCAGTAGAGA TTTGGGATTG GGATTTGACC AGCAGGAATG ACTTCATGGG ATCTTTGTCC
TTTGGGATTT CTGAACTTCA GAAAGCCAGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG
GAGGAAGGCG AGTACTTCAA TGTGCCTGTG CCACCAGAAG GAAGTGAGGC CAATGAAGAA
CTGCGGCAGA AATTTGAGAG GGCCAAGATC AGTCAGGGAA CCAAGGTCCC GGAAGAAAAG
ACGACCAACA CTGTCTCCAA ATTTGACAAC AATGGCAACA GAGACCGGAT GAAACTGACC
GATTTTAACT TCCTAATGGT GCTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTTTCAGAA
CGAAAAGGCA CAGATGAGCT CTATGCTGTG AAGATCCTGA AGAAGGACGT TGTGATCCAA
GATGATGACG TGGAGTGCAC TATGGTGGAG AAGCGGGTGT TGGCCCTGCC TGGGAAGCCG
CCCTTCCTGA CCCAGCTCCA CTCCTGCTTC CAGACCATGG ACCGCCTGTA CTTTGTGATG
GAGTACGTGA ATGGGGGCGA CCTCATGTAT CACATCCAGC AAGTCGGCCG GTTCAAGGAG
CCCCATGCTG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT ACAGAGTAAG
GGCATCATTT ACCGTGACCT AAAACTTGAC AACGTGATGC TCGATTCTGA GGGACACATC
AAGATTGCCG ATTTTGGCAT GTGTAAGGAA AACATCTGGG ATGGGGTGAC AACCAAGACA
TTCTGTGGCA CTCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC CTATGGGAAG
TCCGTGGATT GGTGGGCATT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG GCAGGCACCC
TTTGAAGGGG AGGATGAAGA TGAACTCTTC CAATCCATCA TGGAACACAA CGTAGCCTAT
CCCAAGTCTA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATGAC CAAACACCCA
GGCAAACGTC TGGGTTGTGG ACCTGAAGGC GAACGTGATA TCAAAGAGCA TGCATTTTTC
CGGTATATTG ATTGGGAGAA ACTTGAACGC AAAGAGATCC AGCCCCCTTA TAAGCCAAAA
GCTAGAGACA AGAGAGACAC CTCCAACTTC GACAAAGAGT TCACCAGACA GCCTGTGGAA
CTGACCCCCA CTGATAAACT CTTCATCATG AACTTGGACC AAAATGAATT TGCTGGCTTC
TCTTATACTA ACCCAGAGTT TGTCATTAAT GTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_997700.1
CDS198..2213
Misc Feature(1)30..32(+)
Misc Feature(2)201..203(+)
Misc Feature(3)243..245(+)
Misc Feature(4)246..248(+)
Misc Feature(5)306..455(+)
Misc Feature(6)306..455(+)
Misc Feature(7)327..386(+)
Misc Feature(8)336..344(+)
Misc Feature(9)501..650(+)
Misc Feature(10)501..650(+)
Misc Feature(11)522..581(+)
Misc Feature(12)531..539(+)
Misc Feature(13)672..1064(+)
Misc Feature(14)756..959(+)
Misc Feature(15)1218..2189(+)
Misc Feature(16)1221..1997(+)
Misc Feature(17)1239..1823(+)
Misc Feature(18)1239..1649(+)
Misc Feature(19)1251..1823(+)
Misc Feature(20)1644..1715(+)
Misc Feature(21)1695..1697(+)
Misc Feature(22)1707..1709(+)
Misc Feature(23)2100..2102(+)
Misc Feature(24)2100..2102(+)
Misc Feature(25)2121..2123(+)
Misc Feature(26)2121..2123(+)
Misc Feature(27)2121..2123(+)
Misc Feature(28)2166..2183(+)
Misc Feature(29)2178..2180(+)
Misc Feature(30)2178..2180(+)
Exon (1)1..370
Gene:PRKCB
Gene Synonym:
Exon (2)371..402
Gene:PRKCB
Gene Synonym:
Exon (3)403..485
Gene:PRKCB
Gene Synonym:
Exon (4)486..597
Gene:PRKCB
Gene Synonym:
Exon (5)598..726
Gene:PRKCB
Gene Synonym:
Exon (6)727..883
Gene:PRKCB
Gene Synonym:
Exon (7)884..1018
Gene:PRKCB
Gene Synonym:
Exon (8)1019..1115
Gene:PRKCB
Gene Synonym:
Exon (9)1116..1262
Gene:PRKCB
Gene Synonym:
Exon (10)1263..1436
Gene:PRKCB
Gene Synonym:
Exon (11)1437..1528
Gene:PRKCB
Gene Synonym:
Exon (12)1529..1591
Gene:PRKCB
Gene Synonym:
Exon (13)1592..1730
Gene:PRKCB
Gene Synonym:
Exon (14)1731..1811
Gene:PRKCB
Gene Synonym:
Exon (15)1812..1919
Gene:PRKCB
Gene Synonym:
Exon (16)1920..2060
Gene:PRKCB
Gene Synonym:
Exon (17)2061..2711
Gene:PRKCB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_212535
Organism Homo sapiens (human)
Definition Homo sapiens protein kinase C, beta (PRKCB), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_212535

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGCGAGGGCG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCATGAGG TCAAGAACCA CAAATTCACC
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC
GGGAAGCAGG GATTCCAGTG CCAAGTTTGC TGCTTTGTGG TGCACAAGCG GTGCCATGAA
TTTGTCACAT TCTCCTGCCC TGGCGCTGAC AAGGGTCCAG CCTCCGATGA CCCCCGCAGC
AAACACAAGT TTAAGATCCA CACGTACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCA
CTGCTGTATG GACTCATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT GAATGTGCAC
AAGCGCTGCG TGATGAATGT TCCCAGCCTG TGTGGCACGG ACCACACGGA GCGCCGCGGC
CGCATCTACA TCCAGGCCCA CATCGACAGG GACGTCCTCA TTGTCCTCGT AAGAGATGCT
AAAAACCTTG TACCTATGGA CCCCAATGGC CTGTCAGATC CCTACGTAAA ACTGAAACTG
ATTCCCGATC CCAAAAGTGA GAGCAAACAG AAGACCAAAA CCATCAAATG CTCCCTCAAC
CCTGAGTGGA ATGAGACATT TAGATTTCAG CTGAAAGAAT CGGACAAAGA CAGAAGACTG
TCAGTAGAGA TTTGGGATTG GGATTTGACC AGCAGGAATG ACTTCATGGG ATCTTTGTCC
TTTGGGATTT CTGAACTTCA GAAAGCCAGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG
GAGGAAGGCG AGTACTTCAA TGTGCCTGTG CCACCAGAAG GAAGTGAGGC CAATGAAGAA
CTGCGGCAGA AATTTGAGAG GGCCAAGATC AGTCAGGGAA CCAAGGTCCC GGAAGAAAAG
ACGACCAACA CTGTCTCCAA ATTTGACAAC AATGGCAACA GAGACCGGAT GAAACTGACC
GATTTTAACT TCCTAATGGT GCTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTTTCAGAA
CGAAAAGGCA CAGATGAGCT CTATGCTGTG AAGATCCTGA AGAAGGACGT TGTGATCCAA
GATGATGACG TGGAGTGCAC TATGGTGGAG AAGCGGGTGT TGGCCCTGCC TGGGAAGCCG
CCCTTCCTGA CCCAGCTCCA CTCCTGCTTC CAGACCATGG ACCGCCTGTA CTTTGTGATG
GAGTACGTGA ATGGGGGCGA CCTCATGTAT CACATCCAGC AAGTCGGCCG GTTCAAGGAG
CCCCATGCTG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT ACAGAGTAAG
GGCATCATTT ACCGTGACCT AAAACTTGAC AACGTGATGC TCGATTCTGA GGGACACATC
AAGATTGCCG ATTTTGGCAT GTGTAAGGAA AACATCTGGG ATGGGGTGAC AACCAAGACA
TTCTGTGGCA CTCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC CTATGGGAAG
TCCGTGGATT GGTGGGCATT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG GCAGGCACCC
TTTGAAGGGG AGGATGAAGA TGAACTCTTC CAATCCATCA TGGAACACAA CGTAGCCTAT
CCCAAGTCTA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATGAC CAAACACCCA
GGCAAACGTC TGGGTTGTGG ACCTGAAGGC GAACGTGATA TCAAAGAGCA TGCATTTTTC
CGGTATATTG ATTGGGAGAA ACTTGAACGC AAAGAGATCC AGCCCCCTTA TAAGCCAAAA
GCTAGAGACA AGAGAGACAC CTCCAACTTC GACAAAGAGT TCACCAGACA GCCTGTGGAA
CTGACCCCCA CTGATAAACT CTTCATCATG AACTTGGACC AAAATGAATT TGCTGGCTTC
TCTTATACTA ACCCAGAGTT TGTCATTAAT GTGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Protein kinase C isoforms in atherosclerosis: pro- or anti-inflammatory?
Biochem. Pharmacol. 88 (2), 139-149 (2014)
Fan HC, Fernandez-Hernando C and Lai JH.


book

PKC-beta exacerbates in vitro brain barrier damage in hyperglycemic settings via regulation of RhoA/Rho-kinase/MLC2 pathway
J. Cereb. Blood Flow Metab. 33 (12), 1928-1936 (2013)
Srivastava K, Shao B and Bayraktutan U.


book

Tyrosine phosphorylation on spleen tyrosine kinase (Syk) is differentially regulated in human and murine platelets by protein kinase C isoforms
J. Biol. Chem. 288 (40), 29160-29169 (2013)
Buitrago L, Bhavanasi D, Dangelmaier C, Manne BK, Badolia R, Borgognone A, Tsygankov AY, McKenzie SE and Kunapuli SP.


book

Modulation of Rack-1/PKCbetaII signalling by soluble AbetaPPalpha in SH-SY5Y cells
Curr Alzheimer Res 10 (7), 697-705 (2013)
Buoso E, Biundo F, Lanni C, Aiello S, Grossi S, Schettini G, Govoni S and Racchi M.


book

Role of HIF1alpha and PKCbeta in mediating the effect of oxygen and glucose in a novel wound assay
Microvasc. Res. 88, 61-69 (2013)
Gadad PC, Matthews KH and Knott RM.


book

Cloning and characterization of the major promoter of the human protein kinase C beta gene. Regulation by phorbol esters
J. Biol. Chem. 267 (29), 20804-20810 (1992)
Obeid LM, Blobe GC, Karolak LA and Hannun YA.


book

Differential regulation of glycogen synthase kinase-3 beta by protein kinase C isotypes
J. Biol. Chem. 267 (24), 16878-16882 (1992)
Goode N, Hughes K, Woodgett JR and Parker PJ.


book

Identification of the cAMP-dependent protein kinase and protein kinase C phosphorylation sites within the major intracellular domains of the beta 1, gamma 2S, and gamma 2L subunits of the gamma-aminobutyric acid type A receptor
J. Biol. Chem. 267 (20), 14470-14476 (1992)
Moss SJ, Doherty CA and Huganir RL.


book

Down-regulation of human protein kinase C alpha is associated with terminal neutrophil differentiation
Blood 80 (1), 68-76 (1992)
Devalia V, Thomas NS, Roberts PJ, Jones HM and Linch DC.


book

Positive and negative regulation of the transcription of the human protein kinase C beta gene
J. Biol. Chem. 267 (9), 6158-6163 (1992)
Niino YS, Ohno S and Suzuki K.


 
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