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MAPK8 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MAPK8
Entrez Gene ID 5599
Full Name mitogen-activated protein kinase 8
Synonyms JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c
General protein information
Preferred Names
mitogen-activated protein kinase 8
Names
mitogen-activated protein kinase 8
MAP kinase 8
JUN N-terminal kinase
c-Jun N-terminal kinase 1
stress-activated protein kinase 1
stress-activated protein kinase 1c
mitogen-activated protein kinase 8 isoform JNK1 beta2
mitogen-activated protein kinase 8 isoform JNK1 alpha1
NP_002741.1
NP_620634.1
NP_620635.1
NP_620637.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

10

10q11.22

Summary The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jun 2013]. lac of sum
Disorder MIM:

601158

Disorder Html:





The following MAPK8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAPK8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu22227 XM_011539953 PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu22227 XM_011539954 PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu22227 XM_011539955 PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu22182 XM_011539956 PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu23194 XM_011539957 PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu22909 XM_011539958 PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu22909 NM_002750 Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant JNK1-a1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu23194 NM_139046 Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant JNK1-b1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu22182 NM_139049 Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant JNK1-a2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu22227 NM_001278547 Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant JNK1-b2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu22495 NM_001278548 Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu22227
Accession Version XM_011539953.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product mitogen-activated protein kinase 8 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTTGA CATTTGGTCA GTTGGGTGCA TCATGGGAGA AATGATCAAA
GGTGGTGTTT TGTTCCCAGG TACAGATCAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGGT
GCAGCAGTGA TCAATGGCTC TCAGCATCCA TCATCATCGT CGTCTGTCAA TGATGTGTCT
TCAATGTCAA CAGATCCGAC TTTGGCCTCT GATACAGACA GCAGTCTAGA AGCAGCAGCT
GGGCCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011538255.1
CDS458..1741
Misc Feature(1)530..1537(+)
Misc Feature(2)533..1420(+)
Misc Feature(3)551..1138(+)
Misc Feature(4)551..961(+)
Misc Feature(5)671..1138(+)
Misc Feature(6)791..1444(+)
Misc Feature(7)959..1027(+)
Translation
Position Chain Variation Link
32 32 c, g dbSNP:113540415
36 36 a, g dbSNP:527766384
63 63 a, c dbSNP:12775943
155 155 c, g dbSNP:189523001
174 174 a, g dbSNP:770946197
213 213 a, g dbSNP:749160458
247 247 g, t dbSNP:777149903
262 262 -, tttt dbSNP:781624027
265 265 a, g dbSNP:547786940
349 349 a, g dbSNP:750576068
409 409 a, c dbSNP:764704019
415 415 a, g, t dbSNP:200375054
415 415 -, g dbSNP:746212436
416 416 c, g, t dbSNP:779204208
436 436 a, g dbSNP:199529538
448 448 a, g dbSNP:780993770
451 451 c, t dbSNP:747898436
452 452 a, g dbSNP:769657154
453 453 a, c dbSNP:777670645
457 457 c, g dbSNP:749289588
462 462 a, g dbSNP:770396528
473 473 c, t dbSNP:773780784
474 474 a, g dbSNP:759178320
480 480 a, g dbSNP:771655862
484 484 g, t dbSNP:150753150
488 488 c, t dbSNP:760960935
497 497 a, g dbSNP:764612003
498 498 a, g dbSNP:754396010
505 505 a, g dbSNP:137900446
512 512 a, g dbSNP:765388602
520 520 a, g dbSNP:750494171
521 521 a, g dbSNP:370411166
528 528 a, c dbSNP:780150000
529 529 a, g dbSNP:751861105
531 531 a, g dbSNP:755894470
532 532 a, g dbSNP:745964104
562 562 a, g dbSNP:777584404
565 565 c, t dbSNP:149467709
579 579 a, g dbSNP:770874861
580 580 c, t dbSNP:778730942
581 581 a, g dbSNP:200350498
593 593 a, g dbSNP:143875999
594 594 c, t dbSNP:779657933
600 600 c, t dbSNP:746642373
601 601 c, t dbSNP:768447951
632 632 a, c, t dbSNP:554758335
633 633 a, g dbSNP:573076590
637 637 a, g dbSNP:139947100
718 718 c, t dbSNP:754614629
725 725 a, c dbSNP:780976087
733 733 c, t dbSNP:370630557
738 738 c, g dbSNP:200881923
742 742 a, g dbSNP:770018488
747 747 c, g dbSNP:773674759
748 748 c, t dbSNP:55740545
751 751 -, aga dbSNP:774324400
762 762 a, g dbSNP:771498910
775 775 a, g dbSNP:750668630
786 786 g, t dbSNP:752372488
795 795 c, g dbSNP:755812386
805 805 c, t dbSNP:368826984
844 844 c, t dbSNP:749668518
847 847 c, t dbSNP:771284001
891 891 c, t dbSNP:779380684
916 916 a, g dbSNP:759189891
921 921 a, g dbSNP:755655560
926 926 a, g dbSNP:368198154
931 931 a, g dbSNP:753493053
967 967 c, t dbSNP:199940599
976 976 c, t dbSNP:779292705
983 983 g, t dbSNP:372126348
988 988 a, g dbSNP:772592865
1006 1006 a, g dbSNP:367750354
1018 1018 a, g dbSNP:143505474
1022 1022 c, t dbSNP:202013888
1039 1039 c, t dbSNP:55984396
1048 1048 c, t dbSNP:748354922
1054 1054 c, t dbSNP:553640794
1111 1111 g, t dbSNP:756344637
1121 1121 a, g dbSNP:139339988
1122 1122 g, t dbSNP:749588760
1123 1123 g, t dbSNP:771870957
1129 1129 g, t dbSNP:779762614
1136 1136 a, g dbSNP:746917235
1136 1136 -, g dbSNP:756439086
1141 1141 a, g dbSNP:768508768
1145 1145 c, t dbSNP:576809977
1161 1161 a, g dbSNP:748025349
1202 1202 a, g dbSNP:756073891
1203 1203 c, t dbSNP:377156368
1214 1214 c, t dbSNP:780732703
1216 1216 a, g dbSNP:777194793
1234 1234 c, t dbSNP:748759491
1245 1245 a, g dbSNP:770530313
1247 1247 a, c dbSNP:752183776
1252 1252 a, g dbSNP:201458109
1261 1261 a, g dbSNP:745574142
1262 1262 c, t dbSNP:199509878
1265 1265 a, t dbSNP:758356952
1272 1272 a, c dbSNP:569412118
1279 1279 c, g dbSNP:776020905
1288 1288 c, t dbSNP:761225527
1297 1297 c, t dbSNP:529785308
1369 1369 a, g, t dbSNP:45505100
1373 1373 g, t dbSNP:775931291
1389 1389 c, g dbSNP:747392955
1394 1394 a, g dbSNP:769203778
1396 1396 g, t dbSNP:540242907
1405 1405 c, g dbSNP:373006192
1408 1408 a, g dbSNP:765213856
1414 1414 a, g dbSNP:147168789
1422 1422 a, g dbSNP:142342900
1426 1426 c, t dbSNP:766599034
1441 1441 c, t dbSNP:751835651
1447 1447 a, g dbSNP:755896523
1456 1456 a, g, t dbSNP:574643625
1462 1462 a, g dbSNP:759803140
1464 1464 a, g dbSNP:763747268
1491 1491 a, g dbSNP:753698159
1492 1492 a, g dbSNP:761590637
1493 1493 a, g dbSNP:765234973
1496 1496 c, t dbSNP:750388057
1499 1499 a, g dbSNP:757980939
1501 1501 a, c dbSNP:55859048
1526 1526 c, t dbSNP:201417282
1527 1527 a, g dbSNP:754818843
1537 1537 c, t dbSNP:368000003
1539 1539 c, t dbSNP:371493413
1544 1544 c, t dbSNP:765925330
1550 1550 a, g dbSNP:45483593
1556 1556 a, g dbSNP:28395809
1557 1557 c, g dbSNP:751150249
1558 1558 c, g dbSNP:201573286
1571 1571 a, t dbSNP:767178901
1574 1574 c, t dbSNP:752334346
1575 1575 a, g dbSNP:756498386
1577 1577 a, g dbSNP:778058488
1578 1578 c, g dbSNP:151285118
1579 1579 a, g dbSNP:757766119
1586 1586 g, t dbSNP:778924369
1590 1590 c, g dbSNP:745968638
1608 1608 a, t dbSNP:758497860
1611 1611 a, g dbSNP:780052742
1617 1617 c, t dbSNP:747193295
1623 1623 a, c dbSNP:768761581
1626 1626 -, cat dbSNP:775449388
1627 1627 a, c dbSNP:773097055
1631 1631 g, t dbSNP:749073014
1636 1636 a, g dbSNP:142330800
1639 1639 a, g dbSNP:149971269
1646 1646 -, aat dbSNP:762432631
1646 1646 a, g dbSNP:759451457
1647 1647 a, g dbSNP:367599489
1661 1661 a, c, g dbSNP:774869055
1662 1662 a, t dbSNP:763758430
1669 1669 a, c, g dbSNP:753537417
1674 1674 c, t dbSNP:765655352
1675 1675 a, g dbSNP:750925995
1710 1710 c, g, t dbSNP:147673874
1716 1716 a, c dbSNP:142606131
1720 1720 a, g dbSNP:755161674
1728 1728 a, g dbSNP:781538114
1736 1736 a, g dbSNP:748451475
1748 1748 a, g dbSNP:770839347
1751 1751 c, t dbSNP:778866733
1755 1755 c, t dbSNP:200811159
1756 1756 a, g dbSNP:531537127
1757 1757 a, c, g dbSNP:549658964
1758 1758 a, c, g, t dbSNP:377754613
1760 1760 a, g, t dbSNP:765524640
1779 1779 c, t dbSNP:371191645
1780 1780 a, g, t dbSNP:192957799
1783 1783 c, t dbSNP:755106527
1801 1801 a, c dbSNP:529058910
1847 1847 a, g dbSNP:547238183
1874 1874 a, c dbSNP:566975585
1895 1895 a, g dbSNP:1062225
1895 1895 a, g dbSNP:568806222
1931 1931 c, t dbSNP:184169437
1951 1951 -, t dbSNP:200672731
1959 1959 -, t dbSNP:112248653
1978 1978 a, c dbSNP:572828910
2028 2028 a, t dbSNP:76626172
2046 2046 g, t dbSNP:763956532
2059 2059 a, t dbSNP:146916293
2091 2091 c, t dbSNP:187779968
2104 2104 c, g dbSNP:191604375
2163 2163 a, g dbSNP:757085638
2176 2176 -, ac dbSNP:770438136
2177 2177 -, ac dbSNP:139274132
2178 2178 c, t dbSNP:201539690
2180 2180 a, g dbSNP:184872933
2234 2234 a, g dbSNP:780235762
2239 2239 g, t dbSNP:9284
2282 2282 c, t dbSNP:755050117
2290 2290 g, t dbSNP:76109261
2297 2297 c, t dbSNP:545565494
2341 2341 g, t dbSNP:532658262
2348 2348 a, g dbSNP:139336823
2374 2374 c, t dbSNP:375126595
2375 2375 a, g dbSNP:531207302
2403 2403 c, t dbSNP:772584055
2422 2422 a, g dbSNP:778181154
2425 2425 a, g dbSNP:747375506
2439 2439 g, t dbSNP:141758313
2440 2440 a, g dbSNP:113978751
2502 2502 a, g dbSNP:371660031
2527 2527 a, c dbSNP:529122143
2584 2584 a, g dbSNP:547594710
2618 2618 a, c dbSNP:76130808
2630 2630 -, act dbSNP:367764481
2637 2637 c, t dbSNP:189497037
2647 2647 a, c dbSNP:771479344
2657 2657 a, c dbSNP:552387686
2681 2681 c, t dbSNP:570910132
2684 2684 a, c dbSNP:181440985
2689 2689 c, t dbSNP:556563376
2690 2690 a, g dbSNP:745442429
2747 2747 -, gtt dbSNP:769324510
2773 2773 a, g dbSNP:568793179
2806 2806 -, g dbSNP:34949831
2827 2827 a, c dbSNP:535751875
2853 2853 a, g dbSNP:554378950
2856 2856 a, g dbSNP:572540424
2862 2862 c, t dbSNP:111883599
2863 2863 a, g dbSNP:557572122
2878 2878 a, g dbSNP:764185589
2883 2883 a, g dbSNP:539733371
2905 2905 a, g dbSNP:575693555
2916 2916 a, g dbSNP:762398413
2918 2918 a, t dbSNP:367923445
2921 2921 g, t dbSNP:764063739
2962 2962 a, g dbSNP:73293538
2967 2967 a, g dbSNP:114883745
2984 2984 a, g dbSNP:552488850
3006 3006 c, t dbSNP:113767329
3010 3010 a, g dbSNP:118023498
3021 3021 a, g dbSNP:185363879
3085 3085 c, t dbSNP:8428
3096 3096 c, t dbSNP:757228001
3102 3102 c, t dbSNP:12269230
3126 3126 a, g dbSNP:750223363
3139 3139 c, t dbSNP:78986194
3140 3140 a, g dbSNP:755135892
3150 3150 a, g dbSNP:564586422
3155 3155 c, t dbSNP:73293542
3156 3156 a, g dbSNP:781237853
3161 3161 c, t dbSNP:548305146
3201 3201 -, aa dbSNP:746441213
3212 3212 c, t dbSNP:756583136
3275 3275 a, g dbSNP:550228420
3285 3285 a, t dbSNP:189424334
3309 3309 a, g dbSNP:536186155
3311 3311 c, t dbSNP:778328674
3346 3346 c, t dbSNP:553194777
3348 3348 a, t dbSNP:566320055
3371 3371 a, c dbSNP:73293545
3442 3442 c, t dbSNP:749576084
3469 3469 c, t dbSNP:557898618
3470 3470 a, g dbSNP:181230780
3484 3484 a, g dbSNP:757844029
3499 3499 c, g dbSNP:370067412
3507 3507 g, t dbSNP:186487541
3525 3525 a, g dbSNP:536729925
3538 3538 a, g dbSNP:138542106
3551 3551 a, t dbSNP:573552196
3606 3606 a, t dbSNP:540974327
3637 3637 c, g dbSNP:41281985
3645 3645 a, g dbSNP:769528660
3654 3654 a, g dbSNP:577832760
3700 3700 g, t dbSNP:544897838
3707 3707 a, g dbSNP:775042953
3724 3724 a, g dbSNP:563452535
3726 3726 g, t dbSNP:531876444
3745 3745 c, g dbSNP:550289193
3755 3755 a, c dbSNP:562113759
3781 3781 a, g dbSNP:529277068
3801 3801 a, g dbSNP:377192131
3819 3819 g, t dbSNP:139368250
3822 3822 g, t dbSNP:566261108
3877 3877 c, g dbSNP:149936568
3878 3878 a, g dbSNP:747820046
3936 3936 a, g dbSNP:551592548
3947 3947 a, g dbSNP:144001390
3996 3996 c, t dbSNP:191027325
3997 3997 a, g dbSNP:555046769
4015 4015 a, t dbSNP:774344144
4065 4065 -, t dbSNP:767642856
4066 4066 a, t dbSNP:79326832
4075 4075 -, t dbSNP:59572419
4102 4102 a, g dbSNP:567311884
4108 4108 c, t dbSNP:761675844
4133 4133 -, tta dbSNP:530856944
4193 4193 -, ag dbSNP:770558966
4198 4198 c, g dbSNP:72794390
4240 4240 -, ag dbSNP:759278259
4261 4261 a, g dbSNP:577331562
4278 4278 a, g dbSNP:577888702
4281 4281 c, t dbSNP:760496289
4295 4295 a, g dbSNP:545308444
4308 4308 g, t dbSNP:377506835
4318 4318 a, g dbSNP:557234778
4324 4324 g, t dbSNP:181007127
4331 4331 a, g dbSNP:542798646
4348 4348 a, g dbSNP:142885467
4352 4352 a, g dbSNP:764013495
4378 4378 c, t dbSNP:544811185
4385 4385 g, t dbSNP:539727870
4426 4426 g, t dbSNP:78087466
4486 4486 a, g dbSNP:541091206
4521 4521 a, g dbSNP:559681734
4524 4524 c, t dbSNP:757786822
4534 4534 a, c dbSNP:533493156
4543 4543 a, g dbSNP:376258821
4574 4574 g, t dbSNP:781771632
4628 4628 a, c dbSNP:551653419
4663 4663 -, atc dbSNP:775306117
4665 4665 c, g dbSNP:142367490
4684 4684 c, g dbSNP:746201175
4686 4686 c, t dbSNP:144576948
4690 4690 a, c dbSNP:147957206
4690 4690 -, c dbSNP:760604485
4733 4733 a, c dbSNP:553259839
4763 4763 a, c, g dbSNP:41281987
4779 4779 c, t dbSNP:535389027
4782 4782 c, t dbSNP:552729171
4790 4790 c, t dbSNP:571213818
4821 4821 a, g dbSNP:11101321
4829 4829 -, t dbSNP:113244356
4899 4899 c, t dbSNP:367982902
4928 4928 c, g dbSNP:555072077
4937 4937 a, t dbSNP:748964031
4942 4942 a, g dbSNP:768362872
4948 4948 a, g dbSNP:778337657
4975 4975 a, g dbSNP:575571461
4999 4999 a, g dbSNP:542861947
5021 5021 c, t dbSNP:140590526
5022 5022 c, t dbSNP:759191116
5032 5032 a, c dbSNP:747680094
5040 5040 a, g dbSNP:75124279
5079 5079 a, c dbSNP:773176209
5106 5106 a, g dbSNP:114904314
5133 5133 g, t dbSNP:559533583
5140 5140 a, g dbSNP:186105336
5179 5179 a, g dbSNP:760447448
5188 5188 -, aag dbSNP:753901217
5227 5227 -, tagtt dbSNP:761807523
5243 5243 c, t dbSNP:41281989
5248 5248 c, g dbSNP:190908895
5250 5250 a, g dbSNP:563527484
5267 5267 c, t dbSNP:751787738
5287 5287 c, t dbSNP:531082073
5306 5306 -, aat dbSNP:371168022
5431 5431 c, t dbSNP:776489493
5439 5439 a, g dbSNP:73293546
5442 5442 c, t dbSNP:567832495
5446 5446 c, t dbSNP:763028119
5460 5460 g, t dbSNP:764395640
5473 5473 a, g dbSNP:577052458
5516 5516 a, g dbSNP:143103875
5536 5536 c, t dbSNP:2377350
5537 5537 a, g dbSNP:374580063
5564 5564 a, c, g dbSNP:112893609
5584 5584 a, g dbSNP:538649320
5631 5631 c, t dbSNP:182787752
5632 5632 c, g dbSNP:768112943
5657 5657 c, t dbSNP:569198976
5668 5668 c, t dbSNP:536531143
5719 5719 a, c dbSNP:188723855
5725 5725 a, g dbSNP:138582971
5738 5738 c, g dbSNP:115095528
5767 5767 a, g dbSNP:553441359
5791 5791 c, t dbSNP:141542501
5794 5794 a, g dbSNP:545022869
5797 5797 a, c dbSNP:370768243
5805 5805 a, g dbSNP:756500218
5807 5807 a, g dbSNP:780220395
5824 5824 c, t dbSNP:191727003
5830 5830 c, t dbSNP:368172662
5831 5831 c, t dbSNP:754538589
5847 5847 c, t dbSNP:575568217
5860 5860 a, c dbSNP:145193085
5876 5876 c, t dbSNP:552148955
5880 5880 c, g dbSNP:183263020
5881 5881 c, t dbSNP:528689182
5882 5882 c, t dbSNP:747628568
5889 5889 a, t dbSNP:771626750
5935 5935 a, t dbSNP:777680951
5936 5936 a, t dbSNP:11598320
5986 5986 a, c dbSNP:1048693
5993 5993 c, t dbSNP:564990113
6001 6001 a, g dbSNP:532219887
6007 6007 c, t dbSNP:770914453
6014 6014 g, t dbSNP:74409873
6040 6040 -, aaaa dbSNP:750628929
6043 6043 -, aaag dbSNP:561985709
6047 6047 -, aaaa dbSNP:560909642
6053 6053 -, gtg dbSNP:752035268

Target ORF information:

RefSeq Version XM_011539953
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011539953

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTTGA CATTTGGTCA GTTGGGTGCA TCATGGGAGA AATGATCAAA
GGTGGTGTTT TGTTCCCAGG TACAGATCAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGGT
GCAGCAGTGA TCAATGGCTC TCAGCATCCA TCATCATCGT CGTCTGTCAA TGATGTGTCT
TCAATGTCAA CAGATCCGAC TTTGGCCTCT GATACAGACA GCAGTCTAGA AGCAGCAGCT
GGGCCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22227
Accession Version XM_011539954.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product mitogen-activated protein kinase 8 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTTGA CATTTGGTCA GTTGGGTGCA TCATGGGAGA AATGATCAAA
GGTGGTGTTT TGTTCCCAGG TACAGATCAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGGT
GCAGCAGTGA TCAATGGCTC TCAGCATCCA TCATCATCGT CGTCTGTCAA TGATGTGTCT
TCAATGTCAA CAGATCCGAC TTTGGCCTCT GATACAGACA GCAGTCTAGA AGCAGCAGCT
GGGCCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011538256.1
CDS405..1688
Misc Feature(1)477..1484(+)
Misc Feature(2)480..1367(+)
Misc Feature(3)498..1085(+)
Misc Feature(4)498..908(+)
Misc Feature(5)618..1085(+)
Misc Feature(6)738..1391(+)
Misc Feature(7)906..974(+)
Translation
Position Chain Variation Link
48 48 c, g dbSNP:113540415
52 52 a, g dbSNP:527766384
79 79 a, c dbSNP:12775943
171 171 c, g dbSNP:189523001
190 190 a, g dbSNP:770946197
229 229 a, g dbSNP:749160458
263 263 g, t dbSNP:777149903
278 278 -, tttt dbSNP:781624027
281 281 a, g dbSNP:547786940
356 356 a, c dbSNP:764704019
362 362 a, g, t dbSNP:200375054
362 362 -, g dbSNP:746212436
363 363 c, g, t dbSNP:779204208
383 383 a, g dbSNP:199529538
395 395 a, g dbSNP:780993770
398 398 c, t dbSNP:747898436
399 399 a, g dbSNP:769657154
400 400 a, c dbSNP:777670645
404 404 c, g dbSNP:749289588
409 409 a, g dbSNP:770396528
420 420 c, t dbSNP:773780784
421 421 a, g dbSNP:759178320
427 427 a, g dbSNP:771655862
431 431 g, t dbSNP:150753150
435 435 c, t dbSNP:760960935
444 444 a, g dbSNP:764612003
445 445 a, g dbSNP:754396010
452 452 a, g dbSNP:137900446
459 459 a, g dbSNP:765388602
467 467 a, g dbSNP:750494171
468 468 a, g dbSNP:370411166
475 475 a, c dbSNP:780150000
476 476 a, g dbSNP:751861105
478 478 a, g dbSNP:755894470
479 479 a, g dbSNP:745964104
509 509 a, g dbSNP:777584404
512 512 c, t dbSNP:149467709
526 526 a, g dbSNP:770874861
527 527 c, t dbSNP:778730942
528 528 a, g dbSNP:200350498
540 540 a, g dbSNP:143875999
541 541 c, t dbSNP:779657933
547 547 c, t dbSNP:746642373
548 548 c, t dbSNP:768447951
579 579 a, c, t dbSNP:554758335
580 580 a, g dbSNP:573076590
584 584 a, g dbSNP:139947100
665 665 c, t dbSNP:754614629
672 672 a, c dbSNP:780976087
680 680 c, t dbSNP:370630557
685 685 c, g dbSNP:200881923
689 689 a, g dbSNP:770018488
694 694 c, g dbSNP:773674759
695 695 c, t dbSNP:55740545
698 698 -, aga dbSNP:774324400
709 709 a, g dbSNP:771498910
722 722 a, g dbSNP:750668630
733 733 g, t dbSNP:752372488
742 742 c, g dbSNP:755812386
752 752 c, t dbSNP:368826984
791 791 c, t dbSNP:749668518
794 794 c, t dbSNP:771284001
838 838 c, t dbSNP:779380684
863 863 a, g dbSNP:759189891
868 868 a, g dbSNP:755655560
873 873 a, g dbSNP:368198154
878 878 a, g dbSNP:753493053
914 914 c, t dbSNP:199940599
923 923 c, t dbSNP:779292705
930 930 g, t dbSNP:372126348
935 935 a, g dbSNP:772592865
953 953 a, g dbSNP:367750354
965 965 a, g dbSNP:143505474
969 969 c, t dbSNP:202013888
986 986 c, t dbSNP:55984396
995 995 c, t dbSNP:748354922
1001 1001 c, t dbSNP:553640794
1058 1058 g, t dbSNP:756344637
1068 1068 a, g dbSNP:139339988
1069 1069 g, t dbSNP:749588760
1070 1070 g, t dbSNP:771870957
1076 1076 g, t dbSNP:779762614
1083 1083 a, g dbSNP:746917235
1083 1083 -, g dbSNP:756439086
1088 1088 a, g dbSNP:768508768
1092 1092 c, t dbSNP:576809977
1108 1108 a, g dbSNP:748025349
1149 1149 a, g dbSNP:756073891
1150 1150 c, t dbSNP:377156368
1161 1161 c, t dbSNP:780732703
1163 1163 a, g dbSNP:777194793
1181 1181 c, t dbSNP:748759491
1192 1192 a, g dbSNP:770530313
1194 1194 a, c dbSNP:752183776
1199 1199 a, g dbSNP:201458109
1208 1208 a, g dbSNP:745574142
1209 1209 c, t dbSNP:199509878
1212 1212 a, t dbSNP:758356952
1219 1219 a, c dbSNP:569412118
1226 1226 c, g dbSNP:776020905
1235 1235 c, t dbSNP:761225527
1244 1244 c, t dbSNP:529785308
1316 1316 a, g, t dbSNP:45505100
1320 1320 g, t dbSNP:775931291
1336 1336 c, g dbSNP:747392955
1341 1341 a, g dbSNP:769203778
1343 1343 g, t dbSNP:540242907
1352 1352 c, g dbSNP:373006192
1355 1355 a, g dbSNP:765213856
1361 1361 a, g dbSNP:147168789
1369 1369 a, g dbSNP:142342900
1373 1373 c, t dbSNP:766599034
1388 1388 c, t dbSNP:751835651
1394 1394 a, g dbSNP:755896523
1403 1403 a, g, t dbSNP:574643625
1409 1409 a, g dbSNP:759803140
1411 1411 a, g dbSNP:763747268
1438 1438 a, g dbSNP:753698159
1439 1439 a, g dbSNP:761590637
1440 1440 a, g dbSNP:765234973
1443 1443 c, t dbSNP:750388057
1446 1446 a, g dbSNP:757980939
1448 1448 a, c dbSNP:55859048
1473 1473 c, t dbSNP:201417282
1474 1474 a, g dbSNP:754818843
1484 1484 c, t dbSNP:368000003
1486 1486 c, t dbSNP:371493413
1491 1491 c, t dbSNP:765925330
1497 1497 a, g dbSNP:45483593
1503 1503 a, g dbSNP:28395809
1504 1504 c, g dbSNP:751150249
1505 1505 c, g dbSNP:201573286
1518 1518 a, t dbSNP:767178901
1521 1521 c, t dbSNP:752334346
1522 1522 a, g dbSNP:756498386
1524 1524 a, g dbSNP:778058488
1525 1525 c, g dbSNP:151285118
1526 1526 a, g dbSNP:757766119
1533 1533 g, t dbSNP:778924369
1537 1537 c, g dbSNP:745968638
1555 1555 a, t dbSNP:758497860
1558 1558 a, g dbSNP:780052742
1564 1564 c, t dbSNP:747193295
1570 1570 a, c dbSNP:768761581
1573 1573 -, cat dbSNP:775449388
1574 1574 a, c dbSNP:773097055
1578 1578 g, t dbSNP:749073014
1583 1583 a, g dbSNP:142330800
1586 1586 a, g dbSNP:149971269
1593 1593 -, aat dbSNP:762432631
1593 1593 a, g dbSNP:759451457
1594 1594 a, g dbSNP:367599489
1608 1608 a, c, g dbSNP:774869055
1609 1609 a, t dbSNP:763758430
1616 1616 a, c, g dbSNP:753537417
1621 1621 c, t dbSNP:765655352
1622 1622 a, g dbSNP:750925995
1657 1657 c, g, t dbSNP:147673874
1663 1663 a, c dbSNP:142606131
1667 1667 a, g dbSNP:755161674
1675 1675 a, g dbSNP:781538114
1683 1683 a, g dbSNP:748451475
1695 1695 a, g dbSNP:770839347
1698 1698 c, t dbSNP:778866733
1702 1702 c, t dbSNP:200811159
1703 1703 a, g dbSNP:531537127
1704 1704 a, c, g dbSNP:549658964
1705 1705 a, c, g, t dbSNP:377754613
1707 1707 a, g, t dbSNP:765524640
1726 1726 c, t dbSNP:371191645
1727 1727 a, g, t dbSNP:192957799
1730 1730 c, t dbSNP:755106527
1748 1748 a, c dbSNP:529058910
1794 1794 a, g dbSNP:547238183
1821 1821 a, c dbSNP:566975585
1842 1842 a, g dbSNP:1062225
1842 1842 a, g dbSNP:568806222
1878 1878 c, t dbSNP:184169437
1898 1898 -, t dbSNP:200672731
1906 1906 -, t dbSNP:112248653
1925 1925 a, c dbSNP:572828910
1975 1975 a, t dbSNP:76626172
1993 1993 g, t dbSNP:763956532
2006 2006 a, t dbSNP:146916293
2038 2038 c, t dbSNP:187779968
2051 2051 c, g dbSNP:191604375
2110 2110 a, g dbSNP:757085638
2123 2123 -, ac dbSNP:770438136
2124 2124 -, ac dbSNP:139274132
2125 2125 c, t dbSNP:201539690
2127 2127 a, g dbSNP:184872933
2181 2181 a, g dbSNP:780235762
2186 2186 g, t dbSNP:9284
2229 2229 c, t dbSNP:755050117
2237 2237 g, t dbSNP:76109261
2244 2244 c, t dbSNP:545565494
2288 2288 g, t dbSNP:532658262
2295 2295 a, g dbSNP:139336823
2321 2321 c, t dbSNP:375126595
2322 2322 a, g dbSNP:531207302
2350 2350 c, t dbSNP:772584055
2369 2369 a, g dbSNP:778181154
2372 2372 a, g dbSNP:747375506
2386 2386 g, t dbSNP:141758313
2387 2387 a, g dbSNP:113978751
2449 2449 a, g dbSNP:371660031
2474 2474 a, c dbSNP:529122143
2531 2531 a, g dbSNP:547594710
2565 2565 a, c dbSNP:76130808
2577 2577 -, act dbSNP:367764481
2584 2584 c, t dbSNP:189497037
2594 2594 a, c dbSNP:771479344
2604 2604 a, c dbSNP:552387686
2628 2628 c, t dbSNP:570910132
2631 2631 a, c dbSNP:181440985
2636 2636 c, t dbSNP:556563376
2637 2637 a, g dbSNP:745442429
2694 2694 -, gtt dbSNP:769324510
2720 2720 a, g dbSNP:568793179
2753 2753 -, g dbSNP:34949831
2774 2774 a, c dbSNP:535751875
2800 2800 a, g dbSNP:554378950
2803 2803 a, g dbSNP:572540424
2809 2809 c, t dbSNP:111883599
2810 2810 a, g dbSNP:557572122
2825 2825 a, g dbSNP:764185589
2830 2830 a, g dbSNP:539733371
2852 2852 a, g dbSNP:575693555
2863 2863 a, g dbSNP:762398413
2865 2865 a, t dbSNP:367923445
2868 2868 g, t dbSNP:764063739
2909 2909 a, g dbSNP:73293538
2914 2914 a, g dbSNP:114883745
2931 2931 a, g dbSNP:552488850
2953 2953 c, t dbSNP:113767329
2957 2957 a, g dbSNP:118023498
2968 2968 a, g dbSNP:185363879
3032 3032 c, t dbSNP:8428
3043 3043 c, t dbSNP:757228001
3049 3049 c, t dbSNP:12269230
3073 3073 a, g dbSNP:750223363
3086 3086 c, t dbSNP:78986194
3087 3087 a, g dbSNP:755135892
3097 3097 a, g dbSNP:564586422
3102 3102 c, t dbSNP:73293542
3103 3103 a, g dbSNP:781237853
3108 3108 c, t dbSNP:548305146
3148 3148 -, aa dbSNP:746441213
3159 3159 c, t dbSNP:756583136
3222 3222 a, g dbSNP:550228420
3232 3232 a, t dbSNP:189424334
3256 3256 a, g dbSNP:536186155
3258 3258 c, t dbSNP:778328674
3293 3293 c, t dbSNP:553194777
3295 3295 a, t dbSNP:566320055
3318 3318 a, c dbSNP:73293545
3389 3389 c, t dbSNP:749576084
3416 3416 c, t dbSNP:557898618
3417 3417 a, g dbSNP:181230780
3431 3431 a, g dbSNP:757844029
3446 3446 c, g dbSNP:370067412
3454 3454 g, t dbSNP:186487541
3472 3472 a, g dbSNP:536729925
3485 3485 a, g dbSNP:138542106
3498 3498 a, t dbSNP:573552196
3553 3553 a, t dbSNP:540974327
3584 3584 c, g dbSNP:41281985
3592 3592 a, g dbSNP:769528660
3601 3601 a, g dbSNP:577832760
3647 3647 g, t dbSNP:544897838
3654 3654 a, g dbSNP:775042953
3671 3671 a, g dbSNP:563452535
3673 3673 g, t dbSNP:531876444
3692 3692 c, g dbSNP:550289193
3702 3702 a, c dbSNP:562113759
3728 3728 a, g dbSNP:529277068
3748 3748 a, g dbSNP:377192131
3766 3766 g, t dbSNP:139368250
3769 3769 g, t dbSNP:566261108
3824 3824 c, g dbSNP:149936568
3825 3825 a, g dbSNP:747820046
3883 3883 a, g dbSNP:551592548
3894 3894 a, g dbSNP:144001390
3943 3943 c, t dbSNP:191027325
3944 3944 a, g dbSNP:555046769
3962 3962 a, t dbSNP:774344144
4012 4012 -, t dbSNP:767642856
4013 4013 a, t dbSNP:79326832
4022 4022 -, t dbSNP:59572419
4049 4049 a, g dbSNP:567311884
4055 4055 c, t dbSNP:761675844
4080 4080 -, tta dbSNP:530856944
4140 4140 -, ag dbSNP:770558966
4145 4145 c, g dbSNP:72794390
4187 4187 -, ag dbSNP:759278259
4208 4208 a, g dbSNP:577331562
4225 4225 a, g dbSNP:577888702
4228 4228 c, t dbSNP:760496289
4242 4242 a, g dbSNP:545308444
4255 4255 g, t dbSNP:377506835
4265 4265 a, g dbSNP:557234778
4271 4271 g, t dbSNP:181007127
4278 4278 a, g dbSNP:542798646
4295 4295 a, g dbSNP:142885467
4299 4299 a, g dbSNP:764013495
4325 4325 c, t dbSNP:544811185
4332 4332 g, t dbSNP:539727870
4373 4373 g, t dbSNP:78087466
4433 4433 a, g dbSNP:541091206
4468 4468 a, g dbSNP:559681734
4471 4471 c, t dbSNP:757786822
4481 4481 a, c dbSNP:533493156
4490 4490 a, g dbSNP:376258821
4521 4521 g, t dbSNP:781771632
4575 4575 a, c dbSNP:551653419
4610 4610 -, atc dbSNP:775306117
4612 4612 c, g dbSNP:142367490
4631 4631 c, g dbSNP:746201175
4633 4633 c, t dbSNP:144576948
4637 4637 a, c dbSNP:147957206
4637 4637 -, c dbSNP:760604485
4680 4680 a, c dbSNP:553259839
4710 4710 a, c, g dbSNP:41281987
4726 4726 c, t dbSNP:535389027
4729 4729 c, t dbSNP:552729171
4737 4737 c, t dbSNP:571213818
4768 4768 a, g dbSNP:11101321
4776 4776 -, t dbSNP:113244356
4846 4846 c, t dbSNP:367982902
4875 4875 c, g dbSNP:555072077
4884 4884 a, t dbSNP:748964031
4889 4889 a, g dbSNP:768362872
4895 4895 a, g dbSNP:778337657
4922 4922 a, g dbSNP:575571461
4946 4946 a, g dbSNP:542861947
4968 4968 c, t dbSNP:140590526
4969 4969 c, t dbSNP:759191116
4979 4979 a, c dbSNP:747680094
4987 4987 a, g dbSNP:75124279
5026 5026 a, c dbSNP:773176209
5053 5053 a, g dbSNP:114904314
5080 5080 g, t dbSNP:559533583
5087 5087 a, g dbSNP:186105336
5126 5126 a, g dbSNP:760447448
5135 5135 -, aag dbSNP:753901217
5174 5174 -, tagtt dbSNP:761807523
5190 5190 c, t dbSNP:41281989
5195 5195 c, g dbSNP:190908895
5197 5197 a, g dbSNP:563527484
5214 5214 c, t dbSNP:751787738
5234 5234 c, t dbSNP:531082073
5253 5253 -, aat dbSNP:371168022
5378 5378 c, t dbSNP:776489493
5386 5386 a, g dbSNP:73293546
5389 5389 c, t dbSNP:567832495
5393 5393 c, t dbSNP:763028119
5407 5407 g, t dbSNP:764395640
5420 5420 a, g dbSNP:577052458
5463 5463 a, g dbSNP:143103875
5483 5483 c, t dbSNP:2377350
5484 5484 a, g dbSNP:374580063
5511 5511 a, c, g dbSNP:112893609
5531 5531 a, g dbSNP:538649320
5578 5578 c, t dbSNP:182787752
5579 5579 c, g dbSNP:768112943
5604 5604 c, t dbSNP:569198976
5615 5615 c, t dbSNP:536531143
5666 5666 a, c dbSNP:188723855
5672 5672 a, g dbSNP:138582971
5685 5685 c, g dbSNP:115095528
5714 5714 a, g dbSNP:553441359
5738 5738 c, t dbSNP:141542501
5741 5741 a, g dbSNP:545022869
5744 5744 a, c dbSNP:370768243
5752 5752 a, g dbSNP:756500218
5754 5754 a, g dbSNP:780220395
5771 5771 c, t dbSNP:191727003
5777 5777 c, t dbSNP:368172662
5778 5778 c, t dbSNP:754538589
5794 5794 c, t dbSNP:575568217
5807 5807 a, c dbSNP:145193085
5823 5823 c, t dbSNP:552148955
5827 5827 c, g dbSNP:183263020
5828 5828 c, t dbSNP:528689182
5829 5829 c, t dbSNP:747628568
5836 5836 a, t dbSNP:771626750
5882 5882 a, t dbSNP:777680951
5883 5883 a, t dbSNP:11598320
5933 5933 a, c dbSNP:1048693
5940 5940 c, t dbSNP:564990113
5948 5948 a, g dbSNP:532219887
5954 5954 c, t dbSNP:770914453
5961 5961 g, t dbSNP:74409873
5987 5987 -, aaaa dbSNP:750628929
5990 5990 -, aaag dbSNP:561985709
5994 5994 -, aaaa dbSNP:560909642
6000 6000 -, gtg dbSNP:752035268

Target ORF information:

RefSeq Version XM_011539954
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011539954

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTTGA CATTTGGTCA GTTGGGTGCA TCATGGGAGA AATGATCAAA
GGTGGTGTTT TGTTCCCAGG TACAGATCAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGGT
GCAGCAGTGA TCAATGGCTC TCAGCATCCA TCATCATCGT CGTCTGTCAA TGATGTGTCT
TCAATGTCAA CAGATCCGAC TTTGGCCTCT GATACAGACA GCAGTCTAGA AGCAGCAGCT
GGGCCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22227
Accession Version XM_011539955.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product mitogen-activated protein kinase 8 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTTGA CATTTGGTCA GTTGGGTGCA TCATGGGAGA AATGATCAAA
GGTGGTGTTT TGTTCCCAGG TACAGATCAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGGT
GCAGCAGTGA TCAATGGCTC TCAGCATCCA TCATCATCGT CGTCTGTCAA TGATGTGTCT
TCAATGTCAA CAGATCCGAC TTTGGCCTCT GATACAGACA GCAGTCTAGA AGCAGCAGCT
GGGCCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011538257.1
CDS260..1543
Misc Feature(1)332..1339(+)
Misc Feature(2)335..1222(+)
Misc Feature(3)353..940(+)
Misc Feature(4)353..763(+)
Misc Feature(5)473..940(+)
Misc Feature(6)593..1246(+)
Misc Feature(7)761..829(+)
Translation
Position Chain Variation Link
28 28 c, g dbSNP:113540415
32 32 a, g dbSNP:527766384
59 59 a, c dbSNP:12775943
151 151 a, g dbSNP:750576068
211 211 a, c dbSNP:764704019
217 217 a, g, t dbSNP:200375054
217 217 -, g dbSNP:746212436
218 218 c, g, t dbSNP:779204208
238 238 a, g dbSNP:199529538
250 250 a, g dbSNP:780993770
253 253 c, t dbSNP:747898436
254 254 a, g dbSNP:769657154
255 255 a, c dbSNP:777670645
259 259 c, g dbSNP:749289588
264 264 a, g dbSNP:770396528
275 275 c, t dbSNP:773780784
276 276 a, g dbSNP:759178320
282 282 a, g dbSNP:771655862
286 286 g, t dbSNP:150753150
290 290 c, t dbSNP:760960935
299 299 a, g dbSNP:764612003
300 300 a, g dbSNP:754396010
307 307 a, g dbSNP:137900446
314 314 a, g dbSNP:765388602
322 322 a, g dbSNP:750494171
323 323 a, g dbSNP:370411166
330 330 a, c dbSNP:780150000
331 331 a, g dbSNP:751861105
333 333 a, g dbSNP:755894470
334 334 a, g dbSNP:745964104
364 364 a, g dbSNP:777584404
367 367 c, t dbSNP:149467709
381 381 a, g dbSNP:770874861
382 382 c, t dbSNP:778730942
383 383 a, g dbSNP:200350498
395 395 a, g dbSNP:143875999
396 396 c, t dbSNP:779657933
402 402 c, t dbSNP:746642373
403 403 c, t dbSNP:768447951
434 434 a, c, t dbSNP:554758335
435 435 a, g dbSNP:573076590
439 439 a, g dbSNP:139947100
520 520 c, t dbSNP:754614629
527 527 a, c dbSNP:780976087
535 535 c, t dbSNP:370630557
540 540 c, g dbSNP:200881923
544 544 a, g dbSNP:770018488
549 549 c, g dbSNP:773674759
550 550 c, t dbSNP:55740545
553 553 -, aga dbSNP:774324400
564 564 a, g dbSNP:771498910
577 577 a, g dbSNP:750668630
588 588 g, t dbSNP:752372488
597 597 c, g dbSNP:755812386
607 607 c, t dbSNP:368826984
646 646 c, t dbSNP:749668518
649 649 c, t dbSNP:771284001
693 693 c, t dbSNP:779380684
718 718 a, g dbSNP:759189891
723 723 a, g dbSNP:755655560
728 728 a, g dbSNP:368198154
733 733 a, g dbSNP:753493053
769 769 c, t dbSNP:199940599
778 778 c, t dbSNP:779292705
785 785 g, t dbSNP:372126348
790 790 a, g dbSNP:772592865
808 808 a, g dbSNP:367750354
820 820 a, g dbSNP:143505474
824 824 c, t dbSNP:202013888
841 841 c, t dbSNP:55984396
850 850 c, t dbSNP:748354922
856 856 c, t dbSNP:553640794
913 913 g, t dbSNP:756344637
923 923 a, g dbSNP:139339988
924 924 g, t dbSNP:749588760
925 925 g, t dbSNP:771870957
931 931 g, t dbSNP:779762614
938 938 a, g dbSNP:746917235
938 938 -, g dbSNP:756439086
943 943 a, g dbSNP:768508768
947 947 c, t dbSNP:576809977
963 963 a, g dbSNP:748025349
1004 1004 a, g dbSNP:756073891
1005 1005 c, t dbSNP:377156368
1016 1016 c, t dbSNP:780732703
1018 1018 a, g dbSNP:777194793
1036 1036 c, t dbSNP:748759491
1047 1047 a, g dbSNP:770530313
1049 1049 a, c dbSNP:752183776
1054 1054 a, g dbSNP:201458109
1063 1063 a, g dbSNP:745574142
1064 1064 c, t dbSNP:199509878
1067 1067 a, t dbSNP:758356952
1074 1074 a, c dbSNP:569412118
1081 1081 c, g dbSNP:776020905
1090 1090 c, t dbSNP:761225527
1099 1099 c, t dbSNP:529785308
1171 1171 a, g, t dbSNP:45505100
1175 1175 g, t dbSNP:775931291
1191 1191 c, g dbSNP:747392955
1196 1196 a, g dbSNP:769203778
1198 1198 g, t dbSNP:540242907
1207 1207 c, g dbSNP:373006192
1210 1210 a, g dbSNP:765213856
1216 1216 a, g dbSNP:147168789
1224 1224 a, g dbSNP:142342900
1228 1228 c, t dbSNP:766599034
1243 1243 c, t dbSNP:751835651
1249 1249 a, g dbSNP:755896523
1258 1258 a, g, t dbSNP:574643625
1264 1264 a, g dbSNP:759803140
1266 1266 a, g dbSNP:763747268
1293 1293 a, g dbSNP:753698159
1294 1294 a, g dbSNP:761590637
1295 1295 a, g dbSNP:765234973
1298 1298 c, t dbSNP:750388057
1301 1301 a, g dbSNP:757980939
1303 1303 a, c dbSNP:55859048
1328 1328 c, t dbSNP:201417282
1329 1329 a, g dbSNP:754818843
1339 1339 c, t dbSNP:368000003
1341 1341 c, t dbSNP:371493413
1346 1346 c, t dbSNP:765925330
1352 1352 a, g dbSNP:45483593
1358 1358 a, g dbSNP:28395809
1359 1359 c, g dbSNP:751150249
1360 1360 c, g dbSNP:201573286
1373 1373 a, t dbSNP:767178901
1376 1376 c, t dbSNP:752334346
1377 1377 a, g dbSNP:756498386
1379 1379 a, g dbSNP:778058488
1380 1380 c, g dbSNP:151285118
1381 1381 a, g dbSNP:757766119
1388 1388 g, t dbSNP:778924369
1392 1392 c, g dbSNP:745968638
1410 1410 a, t dbSNP:758497860
1413 1413 a, g dbSNP:780052742
1419 1419 c, t dbSNP:747193295
1425 1425 a, c dbSNP:768761581
1428 1428 -, cat dbSNP:775449388
1429 1429 a, c dbSNP:773097055
1433 1433 g, t dbSNP:749073014
1438 1438 a, g dbSNP:142330800
1441 1441 a, g dbSNP:149971269
1448 1448 -, aat dbSNP:762432631
1448 1448 a, g dbSNP:759451457
1449 1449 a, g dbSNP:367599489
1463 1463 a, c, g dbSNP:774869055
1464 1464 a, t dbSNP:763758430
1471 1471 a, c, g dbSNP:753537417
1476 1476 c, t dbSNP:765655352
1477 1477 a, g dbSNP:750925995
1512 1512 c, g, t dbSNP:147673874
1518 1518 a, c dbSNP:142606131
1522 1522 a, g dbSNP:755161674
1530 1530 a, g dbSNP:781538114
1538 1538 a, g dbSNP:748451475
1550 1550 a, g dbSNP:770839347
1553 1553 c, t dbSNP:778866733
1557 1557 c, t dbSNP:200811159
1558 1558 a, g dbSNP:531537127
1559 1559 a, c, g dbSNP:549658964
1560 1560 a, c, g, t dbSNP:377754613
1562 1562 a, g, t dbSNP:765524640
1581 1581 c, t dbSNP:371191645
1582 1582 a, g, t dbSNP:192957799
1585 1585 c, t dbSNP:755106527
1603 1603 a, c dbSNP:529058910
1649 1649 a, g dbSNP:547238183
1676 1676 a, c dbSNP:566975585
1697 1697 a, g dbSNP:1062225
1697 1697 a, g dbSNP:568806222
1733 1733 c, t dbSNP:184169437
1753 1753 -, t dbSNP:200672731
1761 1761 -, t dbSNP:112248653
1780 1780 a, c dbSNP:572828910
1830 1830 a, t dbSNP:76626172
1848 1848 g, t dbSNP:763956532
1861 1861 a, t dbSNP:146916293
1893 1893 c, t dbSNP:187779968
1906 1906 c, g dbSNP:191604375
1965 1965 a, g dbSNP:757085638
1978 1978 -, ac dbSNP:770438136
1979 1979 -, ac dbSNP:139274132
1980 1980 c, t dbSNP:201539690
1982 1982 a, g dbSNP:184872933
2036 2036 a, g dbSNP:780235762
2041 2041 g, t dbSNP:9284
2084 2084 c, t dbSNP:755050117
2092 2092 g, t dbSNP:76109261
2099 2099 c, t dbSNP:545565494
2143 2143 g, t dbSNP:532658262
2150 2150 a, g dbSNP:139336823
2176 2176 c, t dbSNP:375126595
2177 2177 a, g dbSNP:531207302
2205 2205 c, t dbSNP:772584055
2224 2224 a, g dbSNP:778181154
2227 2227 a, g dbSNP:747375506
2241 2241 g, t dbSNP:141758313
2242 2242 a, g dbSNP:113978751
2304 2304 a, g dbSNP:371660031
2329 2329 a, c dbSNP:529122143
2386 2386 a, g dbSNP:547594710
2420 2420 a, c dbSNP:76130808
2432 2432 -, act dbSNP:367764481
2439 2439 c, t dbSNP:189497037
2449 2449 a, c dbSNP:771479344
2459 2459 a, c dbSNP:552387686
2483 2483 c, t dbSNP:570910132
2486 2486 a, c dbSNP:181440985
2491 2491 c, t dbSNP:556563376
2492 2492 a, g dbSNP:745442429
2549 2549 -, gtt dbSNP:769324510
2575 2575 a, g dbSNP:568793179
2608 2608 -, g dbSNP:34949831
2629 2629 a, c dbSNP:535751875
2655 2655 a, g dbSNP:554378950
2658 2658 a, g dbSNP:572540424
2664 2664 c, t dbSNP:111883599
2665 2665 a, g dbSNP:557572122
2680 2680 a, g dbSNP:764185589
2685 2685 a, g dbSNP:539733371
2707 2707 a, g dbSNP:575693555
2718 2718 a, g dbSNP:762398413
2720 2720 a, t dbSNP:367923445
2723 2723 g, t dbSNP:764063739
2764 2764 a, g dbSNP:73293538
2769 2769 a, g dbSNP:114883745
2786 2786 a, g dbSNP:552488850
2808 2808 c, t dbSNP:113767329
2812 2812 a, g dbSNP:118023498
2823 2823 a, g dbSNP:185363879
2887 2887 c, t dbSNP:8428
2898 2898 c, t dbSNP:757228001
2904 2904 c, t dbSNP:12269230
2928 2928 a, g dbSNP:750223363
2941 2941 c, t dbSNP:78986194
2942 2942 a, g dbSNP:755135892
2952 2952 a, g dbSNP:564586422
2957 2957 c, t dbSNP:73293542
2958 2958 a, g dbSNP:781237853
2963 2963 c, t dbSNP:548305146
3003 3003 -, aa dbSNP:746441213
3014 3014 c, t dbSNP:756583136
3077 3077 a, g dbSNP:550228420
3087 3087 a, t dbSNP:189424334
3111 3111 a, g dbSNP:536186155
3113 3113 c, t dbSNP:778328674
3148 3148 c, t dbSNP:553194777
3150 3150 a, t dbSNP:566320055
3173 3173 a, c dbSNP:73293545
3244 3244 c, t dbSNP:749576084
3271 3271 c, t dbSNP:557898618
3272 3272 a, g dbSNP:181230780
3286 3286 a, g dbSNP:757844029
3301 3301 c, g dbSNP:370067412
3309 3309 g, t dbSNP:186487541
3327 3327 a, g dbSNP:536729925
3340 3340 a, g dbSNP:138542106
3353 3353 a, t dbSNP:573552196
3408 3408 a, t dbSNP:540974327
3439 3439 c, g dbSNP:41281985
3447 3447 a, g dbSNP:769528660
3456 3456 a, g dbSNP:577832760
3502 3502 g, t dbSNP:544897838
3509 3509 a, g dbSNP:775042953
3526 3526 a, g dbSNP:563452535
3528 3528 g, t dbSNP:531876444
3547 3547 c, g dbSNP:550289193
3557 3557 a, c dbSNP:562113759
3583 3583 a, g dbSNP:529277068
3603 3603 a, g dbSNP:377192131
3621 3621 g, t dbSNP:139368250
3624 3624 g, t dbSNP:566261108
3679 3679 c, g dbSNP:149936568
3680 3680 a, g dbSNP:747820046
3738 3738 a, g dbSNP:551592548
3749 3749 a, g dbSNP:144001390
3798 3798 c, t dbSNP:191027325
3799 3799 a, g dbSNP:555046769
3817 3817 a, t dbSNP:774344144
3867 3867 -, t dbSNP:767642856
3868 3868 a, t dbSNP:79326832
3877 3877 -, t dbSNP:59572419
3904 3904 a, g dbSNP:567311884
3910 3910 c, t dbSNP:761675844
3935 3935 -, tta dbSNP:530856944
3995 3995 -, ag dbSNP:770558966
4000 4000 c, g dbSNP:72794390
4042 4042 -, ag dbSNP:759278259
4063 4063 a, g dbSNP:577331562
4080 4080 a, g dbSNP:577888702
4083 4083 c, t dbSNP:760496289
4097 4097 a, g dbSNP:545308444
4110 4110 g, t dbSNP:377506835
4120 4120 a, g dbSNP:557234778
4126 4126 g, t dbSNP:181007127
4133 4133 a, g dbSNP:542798646
4150 4150 a, g dbSNP:142885467
4154 4154 a, g dbSNP:764013495
4180 4180 c, t dbSNP:544811185
4187 4187 g, t dbSNP:539727870
4228 4228 g, t dbSNP:78087466
4288 4288 a, g dbSNP:541091206
4323 4323 a, g dbSNP:559681734
4326 4326 c, t dbSNP:757786822
4336 4336 a, c dbSNP:533493156
4345 4345 a, g dbSNP:376258821
4376 4376 g, t dbSNP:781771632
4430 4430 a, c dbSNP:551653419
4465 4465 -, atc dbSNP:775306117
4467 4467 c, g dbSNP:142367490
4486 4486 c, g dbSNP:746201175
4488 4488 c, t dbSNP:144576948
4492 4492 a, c dbSNP:147957206
4492 4492 -, c dbSNP:760604485
4535 4535 a, c dbSNP:553259839
4565 4565 a, c, g dbSNP:41281987
4581 4581 c, t dbSNP:535389027
4584 4584 c, t dbSNP:552729171
4592 4592 c, t dbSNP:571213818
4623 4623 a, g dbSNP:11101321
4631 4631 -, t dbSNP:113244356
4701 4701 c, t dbSNP:367982902
4730 4730 c, g dbSNP:555072077
4739 4739 a, t dbSNP:748964031
4744 4744 a, g dbSNP:768362872
4750 4750 a, g dbSNP:778337657
4777 4777 a, g dbSNP:575571461
4801 4801 a, g dbSNP:542861947
4823 4823 c, t dbSNP:140590526
4824 4824 c, t dbSNP:759191116
4834 4834 a, c dbSNP:747680094
4842 4842 a, g dbSNP:75124279
4881 4881 a, c dbSNP:773176209
4908 4908 a, g dbSNP:114904314
4935 4935 g, t dbSNP:559533583
4942 4942 a, g dbSNP:186105336
4981 4981 a, g dbSNP:760447448
4990 4990 -, aag dbSNP:753901217
5029 5029 -, tagtt dbSNP:761807523
5045 5045 c, t dbSNP:41281989
5050 5050 c, g dbSNP:190908895
5052 5052 a, g dbSNP:563527484
5069 5069 c, t dbSNP:751787738
5089 5089 c, t dbSNP:531082073
5108 5108 -, aat dbSNP:371168022
5233 5233 c, t dbSNP:776489493
5241 5241 a, g dbSNP:73293546
5244 5244 c, t dbSNP:567832495
5248 5248 c, t dbSNP:763028119
5262 5262 g, t dbSNP:764395640
5275 5275 a, g dbSNP:577052458
5318 5318 a, g dbSNP:143103875
5338 5338 c, t dbSNP:2377350
5339 5339 a, g dbSNP:374580063
5366 5366 a, c, g dbSNP:112893609
5386 5386 a, g dbSNP:538649320
5433 5433 c, t dbSNP:182787752
5434 5434 c, g dbSNP:768112943
5459 5459 c, t dbSNP:569198976
5470 5470 c, t dbSNP:536531143
5521 5521 a, c dbSNP:188723855
5527 5527 a, g dbSNP:138582971
5540 5540 c, g dbSNP:115095528
5569 5569 a, g dbSNP:553441359
5593 5593 c, t dbSNP:141542501
5596 5596 a, g dbSNP:545022869
5599 5599 a, c dbSNP:370768243
5607 5607 a, g dbSNP:756500218
5609 5609 a, g dbSNP:780220395
5626 5626 c, t dbSNP:191727003
5632 5632 c, t dbSNP:368172662
5633 5633 c, t dbSNP:754538589
5649 5649 c, t dbSNP:575568217
5662 5662 a, c dbSNP:145193085
5678 5678 c, t dbSNP:552148955
5682 5682 c, g dbSNP:183263020
5683 5683 c, t dbSNP:528689182
5684 5684 c, t dbSNP:747628568
5691 5691 a, t dbSNP:771626750
5737 5737 a, t dbSNP:777680951
5738 5738 a, t dbSNP:11598320
5788 5788 a, c dbSNP:1048693
5795 5795 c, t dbSNP:564990113
5803 5803 a, g dbSNP:532219887
5809 5809 c, t dbSNP:770914453
5816 5816 g, t dbSNP:74409873
5842 5842 -, aaaa dbSNP:750628929
5845 5845 -, aaag dbSNP:561985709
5849 5849 -, aaaa dbSNP:560909642
5855 5855 -, gtg dbSNP:752035268

Target ORF information:

RefSeq Version XM_011539955
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011539955

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTTGA CATTTGGTCA GTTGGGTGCA TCATGGGAGA AATGATCAAA
GGTGGTGTTT TGTTCCCAGG TACAGATCAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGGT
GCAGCAGTGA TCAATGGCTC TCAGCATCCA TCATCATCGT CGTCTGTCAA TGATGTGTCT
TCAATGTCAA CAGATCCGAC TTTGGCCTCT GATACAGACA GCAGTCTAGA AGCAGCAGCT
GGGCCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22182
Accession Version XM_011539956.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product mitogen-activated protein kinase 8 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTGGA TTTATGGTCT GTGGGGTGCA TTATGGGAGA AATGGTTTGC
CACAAAATCC TCTTTCCAGG AAGGGACTAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGGT
GCAGCAGTGA TCAATGGCTC TCAGCATCCA TCATCATCGT CGTCTGTCAA TGATGTGTCT
TCAATGTCAA CAGATCCGAC TTTGGCCTCT GATACAGACA GCAGTCTAGA AGCAGCAGCT
GGGCCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011538258.1
CDS458..1741
Misc Feature(1)530..1537(+)
Misc Feature(2)533..1420(+)
Misc Feature(3)551..1138(+)
Misc Feature(4)551..961(+)
Misc Feature(5)671..1138(+)
Misc Feature(6)791..1444(+)
Misc Feature(7)959..1027(+)
Translation
Position Chain Variation Link
32 32 c, g dbSNP:113540415
36 36 a, g dbSNP:527766384
63 63 a, c dbSNP:12775943
155 155 c, g dbSNP:189523001
174 174 a, g dbSNP:770946197
213 213 a, g dbSNP:749160458
247 247 g, t dbSNP:777149903
262 262 -, tttt dbSNP:781624027
265 265 a, g dbSNP:547786940
349 349 a, g dbSNP:750576068
409 409 a, c dbSNP:764704019
415 415 a, g, t dbSNP:200375054
415 415 -, g dbSNP:746212436
416 416 c, g, t dbSNP:779204208
436 436 a, g dbSNP:199529538
448 448 a, g dbSNP:780993770
451 451 c, t dbSNP:747898436
452 452 a, g dbSNP:769657154
453 453 a, c dbSNP:777670645
457 457 c, g dbSNP:749289588
462 462 a, g dbSNP:770396528
473 473 c, t dbSNP:773780784
474 474 a, g dbSNP:759178320
480 480 a, g dbSNP:771655862
484 484 g, t dbSNP:150753150
488 488 c, t dbSNP:760960935
497 497 a, g dbSNP:764612003
498 498 a, g dbSNP:754396010
505 505 a, g dbSNP:137900446
512 512 a, g dbSNP:765388602
520 520 a, g dbSNP:750494171
521 521 a, g dbSNP:370411166
528 528 a, c dbSNP:780150000
529 529 a, g dbSNP:751861105
531 531 a, g dbSNP:755894470
532 532 a, g dbSNP:745964104
562 562 a, g dbSNP:777584404
565 565 c, t dbSNP:149467709
579 579 a, g dbSNP:770874861
580 580 c, t dbSNP:778730942
581 581 a, g dbSNP:200350498
593 593 a, g dbSNP:143875999
594 594 c, t dbSNP:779657933
600 600 c, t dbSNP:746642373
601 601 c, t dbSNP:768447951
632 632 a, c, t dbSNP:554758335
633 633 a, g dbSNP:573076590
637 637 a, g dbSNP:139947100
718 718 c, t dbSNP:754614629
725 725 a, c dbSNP:780976087
733 733 c, t dbSNP:370630557
738 738 c, g dbSNP:200881923
742 742 a, g dbSNP:770018488
747 747 c, g dbSNP:773674759
748 748 c, t dbSNP:55740545
751 751 -, aga dbSNP:774324400
762 762 a, g dbSNP:771498910
775 775 a, g dbSNP:750668630
786 786 g, t dbSNP:752372488
795 795 c, g dbSNP:755812386
805 805 c, t dbSNP:368826984
844 844 c, t dbSNP:749668518
847 847 c, t dbSNP:771284001
891 891 c, t dbSNP:779380684
916 916 a, g dbSNP:759189891
921 921 a, g dbSNP:755655560
926 926 a, g dbSNP:368198154
931 931 a, g dbSNP:753493053
967 967 c, t dbSNP:199940599
976 976 c, t dbSNP:779292705
983 983 g, t dbSNP:372126348
988 988 a, g dbSNP:772592865
1006 1006 a, g dbSNP:367750354
1018 1018 a, g dbSNP:143505474
1022 1022 c, t dbSNP:202013888
1039 1039 c, t dbSNP:55984396
1048 1048 c, t dbSNP:748354922
1054 1054 c, t dbSNP:553640794
1075 1075 g, t dbSNP:1126585
1087 1087 a, t dbSNP:1126586
1090 1090 a, g dbSNP:371770117
1093 1093 a, g dbSNP:780629499
1098 1098 g, t dbSNP:747531271
1099 1099 c, t dbSNP:1126587
1124 1124 a, g dbSNP:1126588
1126 1126 c, g, t dbSNP:1126589
1127 1127 c, g dbSNP:1126590
1129 1129 c, g dbSNP:1126591
1134 1134 c, t dbSNP:376010774
1135 1135 a, g dbSNP:770168990
1138 1138 a, t dbSNP:1126592
1140 1140 c, g dbSNP:1126593
1142 1142 a, g dbSNP:774221220
1161 1161 a, g dbSNP:748025349
1202 1202 a, g dbSNP:756073891
1203 1203 c, t dbSNP:377156368
1214 1214 c, t dbSNP:780732703
1216 1216 a, g dbSNP:777194793
1234 1234 c, t dbSNP:748759491
1245 1245 a, g dbSNP:770530313
1247 1247 a, c dbSNP:752183776
1252 1252 a, g dbSNP:201458109
1261 1261 a, g dbSNP:745574142
1262 1262 c, t dbSNP:199509878
1265 1265 a, t dbSNP:758356952
1272 1272 a, c dbSNP:569412118
1279 1279 c, g dbSNP:776020905
1288 1288 c, t dbSNP:761225527
1297 1297 c, t dbSNP:529785308
1369 1369 a, g, t dbSNP:45505100
1373 1373 g, t dbSNP:775931291
1389 1389 c, g dbSNP:747392955
1394 1394 a, g dbSNP:769203778
1396 1396 g, t dbSNP:540242907
1405 1405 c, g dbSNP:373006192
1408 1408 a, g dbSNP:765213856
1414 1414 a, g dbSNP:147168789
1422 1422 a, g dbSNP:142342900
1426 1426 c, t dbSNP:766599034
1441 1441 c, t dbSNP:751835651
1447 1447 a, g dbSNP:755896523
1456 1456 a, g, t dbSNP:574643625
1462 1462 a, g dbSNP:759803140
1464 1464 a, g dbSNP:763747268
1491 1491 a, g dbSNP:753698159
1492 1492 a, g dbSNP:761590637
1493 1493 a, g dbSNP:765234973
1496 1496 c, t dbSNP:750388057
1499 1499 a, g dbSNP:757980939
1501 1501 a, c dbSNP:55859048
1526 1526 c, t dbSNP:201417282
1527 1527 a, g dbSNP:754818843
1537 1537 c, t dbSNP:368000003
1539 1539 c, t dbSNP:371493413
1544 1544 c, t dbSNP:765925330
1550 1550 a, g dbSNP:45483593
1556 1556 a, g dbSNP:28395809
1557 1557 c, g dbSNP:751150249
1558 1558 c, g dbSNP:201573286
1571 1571 a, t dbSNP:767178901
1574 1574 c, t dbSNP:752334346
1575 1575 a, g dbSNP:756498386
1577 1577 a, g dbSNP:778058488
1578 1578 c, g dbSNP:151285118
1579 1579 a, g dbSNP:757766119
1586 1586 g, t dbSNP:778924369
1590 1590 c, g dbSNP:745968638
1608 1608 a, t dbSNP:758497860
1611 1611 a, g dbSNP:780052742
1617 1617 c, t dbSNP:747193295
1623 1623 a, c dbSNP:768761581
1626 1626 -, cat dbSNP:775449388
1627 1627 a, c dbSNP:773097055
1631 1631 g, t dbSNP:749073014
1636 1636 a, g dbSNP:142330800
1639 1639 a, g dbSNP:149971269
1646 1646 -, aat dbSNP:762432631
1646 1646 a, g dbSNP:759451457
1647 1647 a, g dbSNP:367599489
1661 1661 a, c, g dbSNP:774869055
1662 1662 a, t dbSNP:763758430
1669 1669 a, c, g dbSNP:753537417
1674 1674 c, t dbSNP:765655352
1675 1675 a, g dbSNP:750925995
1710 1710 c, g, t dbSNP:147673874
1716 1716 a, c dbSNP:142606131
1720 1720 a, g dbSNP:755161674
1728 1728 a, g dbSNP:781538114
1736 1736 a, g dbSNP:748451475
1748 1748 a, g dbSNP:770839347
1751 1751 c, t dbSNP:778866733
1755 1755 c, t dbSNP:200811159
1756 1756 a, g dbSNP:531537127
1757 1757 a, c, g dbSNP:549658964
1758 1758 a, c, g, t dbSNP:377754613
1760 1760 a, g, t dbSNP:765524640
1779 1779 c, t dbSNP:371191645
1780 1780 a, g, t dbSNP:192957799
1783 1783 c, t dbSNP:755106527
1801 1801 a, c dbSNP:529058910
1847 1847 a, g dbSNP:547238183
1874 1874 a, c dbSNP:566975585
1895 1895 a, g dbSNP:1062225
1895 1895 a, g dbSNP:568806222
1931 1931 c, t dbSNP:184169437
1951 1951 -, t dbSNP:200672731
1959 1959 -, t dbSNP:112248653
1978 1978 a, c dbSNP:572828910
2028 2028 a, t dbSNP:76626172
2046 2046 g, t dbSNP:763956532
2059 2059 a, t dbSNP:146916293
2091 2091 c, t dbSNP:187779968
2104 2104 c, g dbSNP:191604375
2163 2163 a, g dbSNP:757085638
2176 2176 -, ac dbSNP:770438136
2177 2177 -, ac dbSNP:139274132
2178 2178 c, t dbSNP:201539690
2180 2180 a, g dbSNP:184872933
2234 2234 a, g dbSNP:780235762
2239 2239 g, t dbSNP:9284
2282 2282 c, t dbSNP:755050117
2290 2290 g, t dbSNP:76109261
2297 2297 c, t dbSNP:545565494
2341 2341 g, t dbSNP:532658262
2348 2348 a, g dbSNP:139336823
2374 2374 c, t dbSNP:375126595
2375 2375 a, g dbSNP:531207302
2403 2403 c, t dbSNP:772584055
2422 2422 a, g dbSNP:778181154
2425 2425 a, g dbSNP:747375506
2439 2439 g, t dbSNP:141758313
2440 2440 a, g dbSNP:113978751
2502 2502 a, g dbSNP:371660031
2527 2527 a, c dbSNP:529122143
2584 2584 a, g dbSNP:547594710
2618 2618 a, c dbSNP:76130808
2630 2630 -, act dbSNP:367764481
2637 2637 c, t dbSNP:189497037
2647 2647 a, c dbSNP:771479344
2657 2657 a, c dbSNP:552387686
2681 2681 c, t dbSNP:570910132
2684 2684 a, c dbSNP:181440985
2689 2689 c, t dbSNP:556563376
2690 2690 a, g dbSNP:745442429
2747 2747 -, gtt dbSNP:769324510
2773 2773 a, g dbSNP:568793179
2806 2806 -, g dbSNP:34949831
2827 2827 a, c dbSNP:535751875
2853 2853 a, g dbSNP:554378950
2856 2856 a, g dbSNP:572540424
2862 2862 c, t dbSNP:111883599
2863 2863 a, g dbSNP:557572122
2878 2878 a, g dbSNP:764185589
2883 2883 a, g dbSNP:539733371
2905 2905 a, g dbSNP:575693555
2916 2916 a, g dbSNP:762398413
2918 2918 a, t dbSNP:367923445
2921 2921 g, t dbSNP:764063739
2962 2962 a, g dbSNP:73293538
2967 2967 a, g dbSNP:114883745
2984 2984 a, g dbSNP:552488850
3006 3006 c, t dbSNP:113767329
3010 3010 a, g dbSNP:118023498
3021 3021 a, g dbSNP:185363879
3085 3085 c, t dbSNP:8428
3096 3096 c, t dbSNP:757228001
3102 3102 c, t dbSNP:12269230
3126 3126 a, g dbSNP:750223363
3139 3139 c, t dbSNP:78986194
3140 3140 a, g dbSNP:755135892
3150 3150 a, g dbSNP:564586422
3155 3155 c, t dbSNP:73293542
3156 3156 a, g dbSNP:781237853
3161 3161 c, t dbSNP:548305146
3201 3201 -, aa dbSNP:746441213
3212 3212 c, t dbSNP:756583136
3275 3275 a, g dbSNP:550228420
3285 3285 a, t dbSNP:189424334
3309 3309 a, g dbSNP:536186155
3311 3311 c, t dbSNP:778328674
3346 3346 c, t dbSNP:553194777
3348 3348 a, t dbSNP:566320055
3371 3371 a, c dbSNP:73293545
3442 3442 c, t dbSNP:749576084
3469 3469 c, t dbSNP:557898618
3470 3470 a, g dbSNP:181230780
3484 3484 a, g dbSNP:757844029
3499 3499 c, g dbSNP:370067412
3507 3507 g, t dbSNP:186487541
3525 3525 a, g dbSNP:536729925
3538 3538 a, g dbSNP:138542106
3551 3551 a, t dbSNP:573552196
3606 3606 a, t dbSNP:540974327
3637 3637 c, g dbSNP:41281985
3645 3645 a, g dbSNP:769528660
3654 3654 a, g dbSNP:577832760
3700 3700 g, t dbSNP:544897838
3707 3707 a, g dbSNP:775042953
3724 3724 a, g dbSNP:563452535
3726 3726 g, t dbSNP:531876444
3745 3745 c, g dbSNP:550289193
3755 3755 a, c dbSNP:562113759
3781 3781 a, g dbSNP:529277068
3801 3801 a, g dbSNP:377192131
3819 3819 g, t dbSNP:139368250
3822 3822 g, t dbSNP:566261108
3877 3877 c, g dbSNP:149936568
3878 3878 a, g dbSNP:747820046
3936 3936 a, g dbSNP:551592548
3947 3947 a, g dbSNP:144001390
3996 3996 c, t dbSNP:191027325
3997 3997 a, g dbSNP:555046769
4015 4015 a, t dbSNP:774344144
4065 4065 -, t dbSNP:767642856
4066 4066 a, t dbSNP:79326832
4075 4075 -, t dbSNP:59572419
4102 4102 a, g dbSNP:567311884
4108 4108 c, t dbSNP:761675844
4133 4133 -, tta dbSNP:530856944
4193 4193 -, ag dbSNP:770558966
4198 4198 c, g dbSNP:72794390
4240 4240 -, ag dbSNP:759278259
4261 4261 a, g dbSNP:577331562
4278 4278 a, g dbSNP:577888702
4281 4281 c, t dbSNP:760496289
4295 4295 a, g dbSNP:545308444
4308 4308 g, t dbSNP:377506835
4318 4318 a, g dbSNP:557234778
4324 4324 g, t dbSNP:181007127
4331 4331 a, g dbSNP:542798646
4348 4348 a, g dbSNP:142885467
4352 4352 a, g dbSNP:764013495
4378 4378 c, t dbSNP:544811185
4385 4385 g, t dbSNP:539727870
4426 4426 g, t dbSNP:78087466
4486 4486 a, g dbSNP:541091206
4521 4521 a, g dbSNP:559681734
4524 4524 c, t dbSNP:757786822
4534 4534 a, c dbSNP:533493156
4543 4543 a, g dbSNP:376258821
4574 4574 g, t dbSNP:781771632
4628 4628 a, c dbSNP:551653419
4663 4663 -, atc dbSNP:775306117
4665 4665 c, g dbSNP:142367490
4684 4684 c, g dbSNP:746201175
4686 4686 c, t dbSNP:144576948
4690 4690 a, c dbSNP:147957206
4690 4690 -, c dbSNP:760604485
4733 4733 a, c dbSNP:553259839
4763 4763 a, c, g dbSNP:41281987
4779 4779 c, t dbSNP:535389027
4782 4782 c, t dbSNP:552729171
4790 4790 c, t dbSNP:571213818
4821 4821 a, g dbSNP:11101321
4829 4829 -, t dbSNP:113244356
4899 4899 c, t dbSNP:367982902
4928 4928 c, g dbSNP:555072077
4937 4937 a, t dbSNP:748964031
4942 4942 a, g dbSNP:768362872
4948 4948 a, g dbSNP:778337657
4975 4975 a, g dbSNP:575571461
4999 4999 a, g dbSNP:542861947
5021 5021 c, t dbSNP:140590526
5022 5022 c, t dbSNP:759191116
5032 5032 a, c dbSNP:747680094
5040 5040 a, g dbSNP:75124279
5079 5079 a, c dbSNP:773176209
5106 5106 a, g dbSNP:114904314
5133 5133 g, t dbSNP:559533583
5140 5140 a, g dbSNP:186105336
5179 5179 a, g dbSNP:760447448
5188 5188 -, aag dbSNP:753901217
5227 5227 -, tagtt dbSNP:761807523
5243 5243 c, t dbSNP:41281989
5248 5248 c, g dbSNP:190908895
5250 5250 a, g dbSNP:563527484
5267 5267 c, t dbSNP:751787738
5287 5287 c, t dbSNP:531082073
5306 5306 -, aat dbSNP:371168022
5431 5431 c, t dbSNP:776489493
5439 5439 a, g dbSNP:73293546
5442 5442 c, t dbSNP:567832495
5446 5446 c, t dbSNP:763028119
5460 5460 g, t dbSNP:764395640
5473 5473 a, g dbSNP:577052458
5516 5516 a, g dbSNP:143103875
5536 5536 c, t dbSNP:2377350
5537 5537 a, g dbSNP:374580063
5564 5564 a, c, g dbSNP:112893609
5584 5584 a, g dbSNP:538649320
5631 5631 c, t dbSNP:182787752
5632 5632 c, g dbSNP:768112943
5657 5657 c, t dbSNP:569198976
5668 5668 c, t dbSNP:536531143
5719 5719 a, c dbSNP:188723855
5725 5725 a, g dbSNP:138582971
5738 5738 c, g dbSNP:115095528
5767 5767 a, g dbSNP:553441359
5791 5791 c, t dbSNP:141542501
5794 5794 a, g dbSNP:545022869
5797 5797 a, c dbSNP:370768243
5805 5805 a, g dbSNP:756500218
5807 5807 a, g dbSNP:780220395
5824 5824 c, t dbSNP:191727003
5830 5830 c, t dbSNP:368172662
5831 5831 c, t dbSNP:754538589
5847 5847 c, t dbSNP:575568217
5860 5860 a, c dbSNP:145193085
5876 5876 c, t dbSNP:552148955
5880 5880 c, g dbSNP:183263020
5881 5881 c, t dbSNP:528689182
5882 5882 c, t dbSNP:747628568
5889 5889 a, t dbSNP:771626750
5935 5935 a, t dbSNP:777680951
5936 5936 a, t dbSNP:11598320
5986 5986 a, c dbSNP:1048693
5993 5993 c, t dbSNP:564990113
6001 6001 a, g dbSNP:532219887
6007 6007 c, t dbSNP:770914453
6014 6014 g, t dbSNP:74409873
6040 6040 -, aaaa dbSNP:750628929
6043 6043 -, aaag dbSNP:561985709
6047 6047 -, aaaa dbSNP:560909642
6053 6053 -, gtg dbSNP:752035268

Target ORF information:

RefSeq Version XM_011539956
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011539956

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTGGA TTTATGGTCT GTGGGGTGCA TTATGGGAGA AATGGTTTGC
CACAAAATCC TCTTTCCAGG AAGGGACTAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGGT
GCAGCAGTGA TCAATGGCTC TCAGCATCCA TCATCATCGT CGTCTGTCAA TGATGTGTCT
TCAATGTCAA CAGATCCGAC TTTGGCCTCT GATACAGACA GCAGTCTAGA AGCAGCAGCT
GGGCCTCTGG GCTGCTGTAG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23194
Accession Version XM_011539957.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1155bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product mitogen-activated protein kinase 8 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGAGCAGAA GCAAGCGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 
ACAGTCCTGA AACGATATCA GAATTTAAAA CCTATAGGCT CAGGAGCTCA AGGAATAGTA
TGCGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT AAGCCGACCA
TTTCAGAATC AGACTCATGC CAAGCGGGCC TACAGAGAGC TAGTTCTTAT GAAATGTGTT
AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA
TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG
ATGGAGCTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AGATGCTGTG TGGAATCAAG
CACCTTCATT CTGCTGGAAT TATTCATCGG GACTTAAAGC CCAGTAATAT AGTAGTAAAA
TCTGATTGCA CTTTGAAGAT TCTTGACTTC GGTCTGGCCA GGACTGCAGG AACGAGTTTT
ATGATGACGC CTTATGTAGT GACTCGCTAC TACAGAGCAC CCGAGGTCAT CCTTGGCATG
GGCTACAAGG AAAACGTTGA CATTTGGTCA GTTGGGTGCA TCATGGGAGA AATGATCAAA
GGTGGTGTTT TGTTCCCAGG TACAGATCAT ATTGATCAGT GGAATAAAGT TATTGAACAG
CTTGGAACAC CATGTCCTGA ATTCATGAAG AAACTGCAAC CAACAGTAAG GACTTACGTT
GAAAACAGAC CTAAATATGC TGGATATAGC TTTGAGAAAC TCTTCCCTGA TGTCCTTTTC
CCAGCTGACT CAGAACACAA CAAACTTAAA GCCAGTCAGG CAAGGGATTT GTTATCCAAA
ATGCTGGTAA TAGATGCATC TAAAAGGATC TCTGTAGATG AAGCTCTCCA ACACCCGTAC
ATCAATGTCT GGTATGATCC TTCTGAAGCA GAAGCTCCAC CACCAAAGAT CCCTGACAAG
CAGTTAGATG AAAGGGAACA CACAATAGAA GAGTGGAAAG AATTGATATA TAAGGAAGTT
ATGGACTTGG AGGAGAGAAC CAAGAATGGA GTTATACGGG GGCAGCCCTC TCCTTTAGCA
CAGGTGCAGC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011538259.1
CDS458..1612
Misc Feature(1)530..1537(+)
Misc Feature(2)533..1420(+)
Misc Feature(3)551..1138(+)
Misc Feature(4)551..961(+)
Misc Feature(5)671..1138(+)
Misc Feature(6)791..1444(+)
Misc Feature(7)959..1027(+)
Translation
Position Chain Variation Link
32 32 c, g dbSNP:113540415
36 36 a, g dbSNP:527766384
63 63 a, c dbSNP:12775943
155 155 c, g dbSNP:189523001
174 174 a, g dbSNP:770946197
213 213 a, g dbSNP:749160458
247 247 g, t dbSNP:777149903
262 262 -, tttt dbSNP:781624027
265 265 a, g dbSNP:547786940
349 349 a, g dbSNP:750576068
409 409 a, c dbSNP:764704019
415 415 a, g, t dbSNP:200375054
415 415 -, g dbSNP:746212436
416 416 c, g, t dbSNP:779204208
436 436 a, g dbSNP:199529538
448 448 a, g dbSNP:780993770
451 451 c, t dbSNP:747898436
452 452 a, g dbSNP:769657154
453 453 a, c dbSNP:777670645
457 457 c, g dbSNP:749289588
462 462 a, g dbSNP:770396528
473 473 c, t dbSNP:773780784
474 474 a, g dbSNP:759178320
480 480 a, g dbSNP:771655862
484 484 g, t dbSNP:150753150
488 488 c, t dbSNP:760960935
497 497 a, g