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MAP2K1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MAP2K1
Entrez Gene ID 5604
Full Name mitogen-activated protein kinase kinase 1
Synonyms CFC3, MAPKK1, MEK1, MKK1, PRKMK1
General protein information
Preferred Names
dual specificity mitogen-activated protein kinase kinase 1
Names
dual specificity mitogen-activated protein kinase kinase 1
MEK 1
MAPKK 1
MAPK/ERK kinase 1
ERK activator kinase 1
protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
NP_002746.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

15

15q22.1-q22.33

Summary The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

176872

Disorder Html: Cardiofaciocutaneous syndrome, 115150 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011521783 XP_011520085 dual specificity mitogen-activated protein kinase kinase 1 isoform X1
NM_002755 NP_002746 dual specificity mitogen-activated protein kinase kinase 1

KEGG
hsa04012 ErbB signaling pathway
hsa04510 Focal adhesion
hsa04664 Fc epsilon RI signaling pathway
hsa04650 Natural killer cell mediated cytotoxicity
hsa04370 VEGF signaling pathway
hsa04010 MAPK signaling pathway
hsa04660 T cell receptor signaling pathway
hsa04540 Gap junction
hsa04620 Toll-like receptor signaling pathway
hsa04662 B cell receptor signaling pathway
hsa04910 Insulin signaling pathway
hsa04912 GnRH signaling pathway
hsa05212 Pancreatic cancer
hsa05214 Glioma
hsa04810 Regulation of actin cytoskeleton
hsa04720 Long-term potentiation
hsa04730 Long-term depression
hsa05219 Bladder cancer
hsa04916 Melanogenesis
hsa05211 Renal cell carcinoma
hsa05213 Endometrial cancer
hsa05221 Acute myeloid leukemia
hsa04270 Vascular smooth muscle contraction
hsa05220 Chronic myeloid leukemia
hsa05218 Melanoma
hsa05210 Colorectal cancer
hsa05215 Prostate cancer
hsa05223 Non-small cell lung cancer
hsa05200 Pathways in cancer
hsa05216 Thyroid cancer
hsa05020 Prion diseases
hsa04722 Neurotrophin signaling pathway
hsa04320 Dorso-ventral axis formation
hsa04666 Fc gamma R-mediated phagocytosis
hsa04114 Oocyte meiosis
hsa04914 Progesterone-mediated oocyte maturation
hsa04062 Chemokine signaling pathway
hsa04380 Osteoclast differentiation
hsa04725 Cholinergic synapse
hsa05164 Influenza A
hsa04726 Serotonergic synapse
hsa05205 Proteoglycans in cancer
hsa04151 PI3K-Akt signaling pathway
hsa05161 Hepatitis B
hsa04066 HIF-1 signaling pathway
hsa05034 Alcoholism
hsa04915 Estrogen signaling pathway
hsa04068 FoxO signaling pathway
hsa04919 Thyroid hormone signaling pathway
hsa04921 Oxytocin signaling pathway
hsa04022 cGMP-PKG signaling pathway
hsa04024 cAMP signaling pathway
hsa04550 Signaling pathways regulating pluripotency of stem cells
hsa05231 Choline metabolism in cancer
hsa05230 Central carbon metabolism in cancer
hsa05206 MicroRNAs in cancer
hsa04071 Sphingolipid signaling pathway
hsa04668 TNF signaling pathway
hsa04917 Prolactin signaling pathway
hsa_M00687 MAPK (ERK1/2) signaling
hsa04014 Ras signaling pathway
hsa04015 Rap1 signaling pathway
BIOCYC
HUMAN_PWY66-14 MAP kinase cascade
Reactome
R-HSA-1643685 Disease
R-HSA-168256 Immune System
R-HSA-5663205 Infectious disease
R-HSA-5210891 Uptake and function of anthrax toxins
R-HSA-5339562 Uptake and actions of bacterial toxins
R-HSA-168164 Toll Like Receptor 3 (TLR3) Cascade
R-HSA-166166 MyD88-independent TLR3/TLR4 cascade
R-HSA-450294 MAP kinase activation in TLR cascade
R-HSA-975871 MyD88 cascade initiated on plasma membrane
R-HSA-168176 Toll Like Receptor 5 (TLR5) Cascade
R-HSA-168898 Toll-Like Receptors Cascades
R-HSA-168142 Toll Like Receptor 10 (TLR10) Cascade
R-HSA-975138 TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation
R-HSA-168249 Innate Immune System
R-HSA-937061 TRIF-mediated TLR3/TLR4 signaling
R-HSA-168179 Toll Like Receptor TLR1:TLR2 Cascade
R-HSA-168138 Toll Like Receptor 9 (TLR9) Cascade
R-HSA-181438 Toll Like Receptor 2 (TLR2) Cascade
R-HSA-166016 Toll Like Receptor 4 (TLR4) Cascade
R-HSA-168188 Toll Like Receptor TLR6:TLR2 Cascade
R-HSA-2172127 DAP12 interactions
R-HSA-166058 MyD88:Mal cascade initiated on plasma membrane
R-HSA-168181 Toll Like Receptor 7/8 (TLR7/8) Cascade
R-HSA-5674135 MAP2K and MAPK activation
R-HSA-975155 MyD88 dependent cascade initiated on endosome
R-HSA-2454202 Fc epsilon receptor (FCERI) signaling
R-HSA-2871796 FCERI mediated MAPK activation
R-HSA-166054 Activated TLR4 signalling
R-HSA-2424491 DAP12 signaling
R-HSA-1280215 Cytokine Signaling in Immune system
R-HSA-5673001 RAF/MAP kinase cascade
R-HSA-5673000 RAF activation
R-HSA-5674499 Negative feedback regulation of MAPK pathway
R-HSA-512988 Interleukin-3, 5 and GM-CSF signaling
R-HSA-446652 Interleukin-1 signaling
R-HSA-5675221 Negative regulation of MAPK pathway
R-HSA-449147 Signaling by Interleukins
R-HSA-451927 Interleukin-2 signaling
R-HSA-912526 Interleukin receptor SHC signaling
R-HSA-162582 Signal Transduction
R-HSA-179812 GRB2 events in EGFR signaling
R-HSA-180336 SHC1 events in EGFR signaling
R-HSA-5654693 FRS-mediated FGFR1 signaling
R-HSA-5654687 Downstream signaling of activated FGFR1
R-HSA-177929 Signaling by EGFR
R-HSA-5654738 Signaling by FGFR2
R-HSA-190236 Signaling by FGFR
R-HSA-5654700 FRS-mediated FGFR2 signaling
R-HSA-5654736 Signaling by FGFR1
R-HSA-5654743 Signaling by FGFR4
R-HSA-74751 Insulin receptor signalling cascade
R-HSA-112399 IRS-mediated signalling
R-HSA-112412 SOS-mediated signalling
R-HSA-5654741 Signaling by FGFR3
R-HSA-5654708 Downstream signaling of activated FGFR3
R-HSA-74752 Signaling by Insulin receptor
R-HSA-166520 Signalling by NGF
R-HSA-187037 NGF signalling via TRKA from the plasma membrane
R-HSA-5654706 FRS-mediated FGFR3 signaling
R-HSA-187687 Signalling to ERKs
R-HSA-5654716 Downstream signaling of activated FGFR4
R-HSA-5654696 Downstream signaling of activated FGFR2
R-HSA-5654712 FRS-mediated FGFR4 signaling
R-HSA-186763 Downstream signal transduction
R-HSA-186797 Signaling by PDGF
R-HSA-4420097 VEGFA-VEGFR2 Pathway
R-HSA-169893 Prolonged ERK activation events
R-HSA-170984 ARMS-mediated activation
R-HSA-194138 Signaling by VEGF
R-HSA-170968 Frs2-mediated activation
R-HSA-1250196 SHC1 events in ERBB2 signaling
R-HSA-1433557 Signaling by SCF-KIT
R-HSA-167044 Signalling to RAS
R-HSA-1250347 SHC1 events in ERBB4 signaling
R-HSA-1227986 Signaling by ERBB2
R-HSA-5683057 MAPK family signaling cascades
R-HSA-187706 Signalling to p38 via RIT and RIN
R-HSA-1236394 Signaling by ERBB4
R-HSA-5218921 VEGFR2 mediated cell proliferation
R-HSA-112409 RAF-independent MAPK1/3 activation
R-HSA-110056 MAPK3 (ERK1) activation
R-HSA-5684996 MAPK1/MAPK3 signaling
R-HSA-1963640 GRB2 events in ERBB2 signaling
R-HSA-372790 Signaling by GPCR
R-HSA-881907 Gastrin-CREB signalling pathway via PKC and MAPK
R-HSA-2586552 Signaling by Leptin
R-HSA-2428928 IRS-related events triggered by IGF1R
R-HSA-2404192 Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
R-HSA-2428924 IGF1R signaling cascade
R-HSA-1266738 Developmental Biology
R-HSA-375165 NCAM signaling for neurite out-growth
R-HSA-373760 L1CAM interactions
R-HSA-445144 Signal transduction by L1
R-HSA-422475 Axon guidance
Pathway Interaction Database
fak_pathway Signaling events mediated by focal adhesion kinase
hedgehog_glipathway Hedgehog signaling events mediated by Gli proteins
met_pathway Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
foxm1pathway FOXM1 transcription factor network
tcrraspathway Ras signaling in the CD4+ TCR pathway
erbb2erbb3pathway ErbB2/ErbB3 signaling events
endothelinpathway Endothelins
trkrpathway Neurotrophic factor-mediated Trk receptor signaling
ceramide_pathway Ceramide signaling pathway
ar_nongenomic_pathway Nongenotropic Androgen signaling
ifngpathway IFN-gamma pathway
il2_1pathway IL2-mediated signaling events
cxcr3pathway CXCR3-mediated signaling events
bcr_5pathway BCR signaling pathway
mtor_4pathway mTOR signaling pathway
ephbfwdpathway EPHB forward signaling
cd8tcrdownstreampathway Downstream signaling in naive CD8+ T cells
fcer1pathway Fc-epsilon receptor I signaling in mast cells
kitpathway Signaling events mediated by Stem cell factor receptor (c-Kit)
erbb1_downstream_pathway ErbB1 downstream signaling
pi3kcibpathway Class IB PI3K non-lipid kinase events
mapktrkpathway Trk receptor signaling mediated by the MAPK pathway
anthraxpathway Cellular roles of Anthrax toxin
WikiPathways
WP382 MAPK signaling pathway
WP1434 Osteopontin Signaling
WP1544 MicroRNAs in cardiomyocyte hypertrophy
WP666 Hypothetical Network for Drug Addiction
WP615 Senescence and Autophagy
WP185 Integrin-mediated cell adhesion
WP304 Kit Receptor Signaling Pathway
WP673 ErbB signaling pathway
WP481 Insulin Signaling
WP437 EGFR1 Signaling Pathway
WP422 MAPK Cascade
WP22 IL-9 Signaling Pathway
WP712 Estrogen signaling pathway
WP734 Serotonin Receptor 4/6/7 and NR3C Signaling
WP75 Toll-like receptor signaling pathway
WP69 TCR Signaling Pathway
WP23 B Cell Receptor Signaling Pathway
WP51 Regulation of Actin Cytoskeleton
WP286 IL-3 Signaling Pathway
WP49 IL-2 Signaling pathway
WP205 IL-7 Signaling Pathway
WP732 Serotonin Receptor 2 and ELK-SRF/GATA4 signaling
WP364 IL-6 Signaling Pathway
WP581 EPO Receptor Signaling
WP259 Nifedipine Activity
WP2377 Integrated Pancreatic Cancer Pathway
WP2374 Oncostatin M Signaling Pathway
WP2380 BDNF signaling pathway
WP2263 Prostate Cancer
WP2018 RANKL/RANK Signaling Pathway
WP2034 Leptin signaling pathway
WP2261 Signaling Pathways in Glioblastoma
WP2037 Prolactin Signaling Pathway
WP2203 TSLP Signaling Pathway
WP2032 TSH signaling pathway
WP2197 Endothelin
WP2324 AGE/RAGE pathway
WP1449 Regulation of toll-like receptor signaling pathway
WP2355 Corticotropin-releasing hormone


Homo sapiens (human) MAP2K1 NP_002746.1
Pan troglodytes (chimpanzee) MAP2K1 NP_001009071.1
Macaca mulatta (Rhesus monkey) MAP2K1 XP_001110225.1
Canis lupus familiaris (dog) MAP2K1 NP_001041559.1
Bos taurus (cattle) MAP2K1 NP_001124224.1
Mus musculus (house mouse) Map2k1 NP_032953.1
Rattus norvegicus (Norway rat) Map2k1 NP_113831.1
Gallus gallus (chicken) MAP2K1 NP_001005830.1
Danio rerio (zebrafish) map2k1 NP_998584.2
Drosophila melanogaster (fruit fly) Dsor1 NP_511098.1
Caenorhabditis elegans mek-2 NP_491087.1
Arabidopsis thaliana (thale cress) MEK1 NP_974619.1
Arabidopsis thaliana (thale cress) MKK2 NP_194710.1
Xenopus (Silurana) tropicalis (western clawed frog) map2k1 NP_001008058.1

Component

ID Name Evidence
GO:0005625 soluble fraction IEA
GO:0005737 cytoplasm IDA
GO:0005794 Golgi apparatus IEA
GO:0005829 cytosol EXP
GO:0005829 cytosol TAS
GO:0005874 microtubule IEA
GO:0005886 plasma membrane IDA
GO:0005938 cell cortex IEA
GO:0032839 dendrite cytoplasm IEA
GO:0033267 axon part IEA
GO:0043204 perikaryon IEA
GO:0048471 perinuclear region of cytoplasm IEA

Function

ID Name Evidence
GO:0000166 nucleotide binding IEA
GO:0004672 protein kinase activity TAS
GO:0004674 protein serine/threonine kinase activity EXP
GO:0004708 MAP kinase kinase activity IEA
GO:0004713 protein tyrosine kinase activity IEA
GO:0004728 receptor signaling protein tyrosine phosphatase activity IEA
GO:0005515 protein binding IPI
GO:0005524 ATP binding IEA
GO:0017016 Ras GTPase binding IEA
GO:0031435 mitogen-activated protein kinase kinase kinase binding IEA

Process

ID Name Evidence
GO:0000165 MAPKKK cascade TAS
GO:0000186 activation of MAPKK activity TAS
GO:0000187 activation of MAPK activity TAS
GO:0002224 toll-like receptor signaling pathway TAS
GO:0002755 MyD88-dependent toll-like receptor signaling pathway EXP
GO:0002755 MyD88-dependent toll-like receptor signaling pathway TAS
GO:0002756 MyD88-independent toll-like receptor signaling pathway EXP
GO:0002756 MyD88-independent toll-like receptor signaling pathway TAS
GO:0003056 regulation of vascular smooth muscle contraction IEA
GO:0006928 cellular component movement TAS
GO:0006935 chemotaxis TAS
GO:0006979 response to oxidative stress IEA
GO:0007050 cell cycle arrest IMP
GO:0007067 mitosis IEA
GO:0007165 signal transduction TAS
GO:0007173 epidermal growth factor receptor signaling pathway TAS
GO:0007264 small GTPase mediated signal transduction EXP
GO:0007265 Ras protein signal transduction EXP
GO:0007411 axon guidance TAS
GO:0008063 Toll signaling pathway TAS
GO:0008285 negative regulation of cell proliferation IDA
GO:0008286 insulin receptor signaling pathway TAS
GO:0030182 neuron differentiation IEA
GO:0030216 keratinocyte differentiation IEA
GO:0030335 positive regulation of cell migration IEA
GO:0032320 positive regulation of Ras GTPase activity IEA
GO:0032402 melanosome transport IEA
GO:0032968 positive regulation of transcription elongation from RNA polymerase II promoter IEA
GO:0034111 negative regulation of homotypic cell-cell adhesion IEA
GO:0034130 toll-like receptor 1 signaling pathway TAS
GO:0034134 toll-like receptor 2 signaling pathway TAS
GO:0034138 toll-like receptor 3 signaling pathway TAS
GO:0034142 toll-like receptor 4 signaling pathway TAS
GO:0045087 innate immune response TAS
GO:0045597 positive regulation of cell differentiation IEA
GO:0047496 vesicle transport along microtubule IEA
GO:0048011 nerve growth factor receptor signaling pathway TAS
GO:0048313 Golgi inheritance IEA
GO:0048678 response to axon injury IEA
GO:0048812 neuron projection morphogenesis IEA
GO:0048870 cell motility IEA
GO:0051291 protein heterooligomerization IEA
GO:0051384 response to glucocorticoid stimulus IEA
GO:0051403 stress-activated MAPK cascade TAS
GO:0060674 placenta blood vessel development IEA
GO:0060711 labyrinthine layer development IEA
GO:0090398 cellular senescence IMP

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAP2K1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP2K1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu59946 XM_011521783 PREDICTED: Homo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu27418
NM_002755 Homo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $189.50
$379.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu59946
Clone ID Related Accession (Same CDS sequence) XM_011521783
Accession Version XM_011521783.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1116bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product dual specificity mitogen-activated protein kinase kinase 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAAGCTGG AGAGGACCAA CTTGGAGGCC TTGCAGAAGA AGCTGGAGGA GCTAGAGCTT 
GATGAGCAGC AGCGAAAGCG CCTTGAGGCC TTTCTTACCC AGAAGCAGAA GGTGGGAGAA
CTGAAGGATG ACGACTTTGA GAAGATCAGT GAGCTGGGGG CTGGCAATGG CGGTGTGGTG
TTCAAGGTCT CCCACAAGCC TTCTGGCCTG GTCATGGCCA GAAAGCTAAT TCATCTGGAG
ATCAAACCCG CAATCCGGAA CCAGATCATA AGGGAGCTGC AGGTTCTGCA TGAGTGCAAC
TCTCCGTACA TCGTGGGCTT CTATGGTGCG TTCTACAGCG ATGGCGAGAT CAGTATCTGC
ATGGAGCACA TGGATGGAGG TTCTCTGGAT CAAGTCCTGA AGAAAGCTGG AAGAATTCCT
GAACAAATTT TAGGAAAAGT TAGCATTGCT GTAATAAAAG GCCTGACATA TCTGAGGGAG
AAGCACAAGA TCATGCACAG AGATGTCAAG CCCTCCAACA TCCTAGTCAA CTCCCGTGGG
GAGATCAAGC TCTGTGACTT TGGGGTCAGC GGGCAGCTCA TCGACTCCAT GGCCAACTCC
TTCGTGGGCA CAAGGTCCTA CATGTCGCCA GAAAGACTCC AGGGGACTCA TTACTCTGTG
CAGTCAGACA TCTGGAGCAT GGGACTGTCT CTGGTAGAGA TGGCGGTTGG GAGGTATCCC
ATCCCTCCTC CAGATGCCAA GGAGCTGGAG CTGATGTTTG GGTGCCAGGT GGAAGGAGAT
GCGGCTGAGA CCCCACCCAG GCCAAGGACC CCCGGGAGGC CCCTTAGCTC ATACGGAATG
GACAGCCGAC CTCCCATGGC AATTTTTGAG TTGTTGGATT ACATAGTCAA CGAGCCTCCT
CCAAAACTGC CCAGTGGAGT GTTCAGTCTG GAATTTCAAG ATTTTGTGAA TAAATGCTTA
ATAAAAAACC CCGCAGAGAG AGCAGATTTG AAGCAACTCA TGGTTCATGC TTTTATCAAG
AGATCTGATG CTGAGGAAGT GGATTTTGCA GGTTGGCTCT GCTCCACCAT CGGCCTTAAC
CAGCCCAGCA CACCAACCCA TGCTGCTGGC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011520085.1
CDS67..1182
Misc Feature(1)184..1140(+)
Misc Feature(2)202..1083(+)
Misc Feature(3)220..792(+)
Misc Feature(4)220..624(+)
Misc Feature(5)229..792(+)
Misc Feature(6)229..657(+)
Misc Feature(7)241..957(+)
Misc Feature(8)619..684(+)
Misc Feature(9)661..954(+)
Translation
Position Chain Variation Link
49 49 c, t dbSNP:368696830
53 53 -, tcggg dbSNP:770726597
60 60 a, g dbSNP:768802655
61 61 a, g dbSNP:772936118
64 64 a, g dbSNP:748965154
65 65 -, a dbSNP:760607376
87 87 c, t dbSNP:755038798
96 96 c, t dbSNP:781144142
118 118 -, c dbSNP:759503971
124 124 c, t dbSNP:397516789
126 126 c, t dbSNP:748100663
147 147 a, c dbSNP:756073029
156 156 c, t dbSNP:147489724
158 158 c, t dbSNP:121908594
162 162 g, t dbSNP:748988504
165 165 c, g dbSNP:770743929
169 169 a, c dbSNP:397516790
177 177 a, g dbSNP:773928828
179 179 g, t dbSNP:730880501
198 198 c, t dbSNP:56200325
199 199 a, g dbSNP:727504317
215 215 a, g dbSNP:547530008
237 237 c, t dbSNP:148656020
251 251 a, g dbSNP:774932586
263 263 a, g dbSNP:762354445
264 264 a, g dbSNP:7494832
275 275 g, t dbSNP:397516791
276 276 c, g dbSNP:377323150
277 277 a, g dbSNP:151207265
279 279 c, t dbSNP:140387057
280 280 a, g dbSNP:368143400
281 281 g, t dbSNP:762110054
286 286 a, g dbSNP:751770209
308 308 g, t dbSNP:730880502
315 315 c, t dbSNP:144166521
316 316 a, g dbSNP:752994941
323 323 a, g dbSNP:727504819
333 333 c, t dbSNP:764248591
355 355 c, t dbSNP:730880503
357 357 c, t dbSNP:753763967
365 365 a, g dbSNP:757254963
368 368 c, g dbSNP:778650731
371 371 c, t dbSNP:397516792
372 372 a, g dbSNP:750270430
378 378 c, t dbSNP:369401783
383 383 gc, tt dbSNP:730880508
383 383 g, t dbSNP:121908596
388 388 c, t dbSNP:397516793
389 389 a, g dbSNP:121908595
396 396 a, g, t dbSNP:139364105
405 405 c, t dbSNP:767989355
411 411 a, c, t dbSNP:146415623
412 412 a, g dbSNP:730880504
427 427 a, g dbSNP:533695080
434 434 a, g dbSNP:771604017
439 439 c, g dbSNP:755707394
441 441 c, t dbSNP:779497093
453 453 -, g dbSNP:755398652
466 466 a, g dbSNP:199504372
469 469 a, g dbSNP:201208360
471 471 a, g dbSNP:200487483
477 477 a, c dbSNP:772312180
492 492 a, g dbSNP:140822439
495 495 g, t dbSNP:747171317
516 516 c, t dbSNP:768918234
549 549 a, g dbSNP:747352922
554 554 a, g dbSNP:769044712
565 565 a, c dbSNP:776763061
592 592 c, g dbSNP:774278448
601 601 c, t dbSNP:759605592
606 606 a, g dbSNP:771990100
608 608 a, g dbSNP:727503996
615 615 a, g dbSNP:775467166
624 624 c, t dbSNP:191996882
627 627 c, t dbSNP:144639340
631 631 a, g dbSNP:727505206
636 636 c, t dbSNP:138466751
648 648 c, t dbSNP:148968935
649 649 a, g dbSNP:761324501
657 657 c, g dbSNP:730880506
711 711 a, g dbSNP:17586159
724 724 a, g dbSNP:761435303
726 726 g, t dbSNP:373745627
729 729 a, g dbSNP:772752167
730 730 g, t dbSNP:730880507
763 763 c, g dbSNP:760016123
771 771 a, g dbSNP:767965126
772 772 a, g dbSNP:753236280
774 774 c, t dbSNP:756434943
792 792 g, t dbSNP:764379969
801 801 a, t dbSNP:754086223
803 803 c, t dbSNP:757459909
804 804 c, g dbSNP:371652992
810 810 a, g dbSNP:745891583
834 834 a, g dbSNP:148151221
844 844 a, g dbSNP:780066304
848 848 c, t dbSNP:144080051
862 862 c, t dbSNP:768480988
864 864 c, t dbSNP:776361123
871 871 a, g dbSNP:747807884
874 874 a, g dbSNP:769383345
875 875 c, g dbSNP:397516794
879 879 c, t dbSNP:370521459
882 882 g, t dbSNP:762411863
900 900 c, t dbSNP:147333830
901 901 a, g dbSNP:773890369
916 916 c, t dbSNP:761222249
927 927 a, t dbSNP:146869577
932 932 c, t dbSNP:777224813
957 957 c, t dbSNP:143019052
960 960 -, g dbSNP:756917248
961 961 c, t dbSNP:771613524
969 969 a, c dbSNP:776941883
970 970 a, c dbSNP:762436311
978 978 a, c dbSNP:538987451
984 984 a, t dbSNP:773755873
987 987 a, g, t dbSNP:763249437
997 997 c, g dbSNP:749645202
1000 1000 g, t dbSNP:751840710
1006 1006 c, g dbSNP:754765552
1008 1008 g, t dbSNP:767650713
1019 1019 a, c dbSNP:184970289
1029 1029 -, aaaa dbSNP:775355010
1034 1034 a, g dbSNP:760766158
1038 1038 c, t dbSNP:547178339
1039 1039 a, g dbSNP:746354996
1051 1051 a, g, t dbSNP:373210982
1062 1062 a, c dbSNP:750035241
1069 1069 g, t dbSNP:558326525
1098 1098 c, t dbSNP:200293968
1101 1101 g, t dbSNP:770847542
1107 1107 a, g dbSNP:537811569
1109 1109 a, c dbSNP:759243614
1113 1113 c, t dbSNP:767237502
1123 1123 a, c, t dbSNP:752538915
1130 1130 c, t dbSNP:371140798
1135 1135 a, g dbSNP:373794940
1137 1137 c, t dbSNP:150841154
1138 1138 a, g dbSNP:750934083
1139 1139 a, g dbSNP:28730804
1143 1143 a, t dbSNP:758866695
1163 1163 c, t dbSNP:780370635
1167 1167 c, t dbSNP:751868729
1168 1168 a, g dbSNP:730880500
1176 1176 c, t dbSNP:781328871
1177 1177 a, g dbSNP:200442489
1181 1181 a, c dbSNP:756208132
1199 1199 a, t dbSNP:777783461
1205 1205 c, t dbSNP:376181437
1208 1208 c, g dbSNP:749220939
1212 1212 c, t dbSNP:771041748
1213 1213 c, t dbSNP:774452900
1218 1218 c, g, t dbSNP:574828384
1219 1219 a, g dbSNP:775193561
1222 1222 a, g dbSNP:540533521
1224 1224 c, t dbSNP:763625326
1227 1227 a, g dbSNP:776051290
1230 1230 a, g dbSNP:761330415
1232 1232 a, g dbSNP:766828156
1234 1234 a, c, t dbSNP:372452427
1235 1235 a, g dbSNP:755282867
1240 1240 -, g dbSNP:756795713
1245 1245 c, t dbSNP:767930298
1246 1246 g, t dbSNP:752962298
1248 1248 c, t dbSNP:756331255
1254 1254 a, g dbSNP:369901206
1261 1261 g, t dbSNP:777889018
1263 1263 g, t dbSNP:749280724
1264 1264 c, t dbSNP:576922968
1268 1268 g, t dbSNP:757416394
1273 1273 c, t dbSNP:778934345
1278 1278 a, g dbSNP:757367010
1283 1283 -, c dbSNP:778371173
1309 1309 a, g dbSNP:771988141
1330 1330 -, tt dbSNP:745540522
1338 1338 c, t dbSNP:373707256
1435 1435 c, t dbSNP:546295504
1441 1441 a, c dbSNP:181822467
1469 1469 a, g dbSNP:184860087
1490 1490 a, c dbSNP:14303
1521 1521 g, t dbSNP:371021779
1525 1525 c, t dbSNP:372877813
1540 1540 a, g dbSNP:561736994
1571 1571 a, c dbSNP:528023985
1582 1582 c, t dbSNP:772667633
1586 1586 c, t dbSNP:190094372
1607 1607 a, g, t dbSNP:56366941
1638 1638 c, t dbSNP:181853123
1712 1712 c, t dbSNP:552268563
1724 1724 c, g dbSNP:747416216
1772 1772 a, g dbSNP:568999981
1774 1774 a, g dbSNP:147974693
1826 1826 a, g dbSNP:375853830
1839 1839 a, g dbSNP:8684
1872 1872 -, tt dbSNP:570295657
1874 1874 c, t dbSNP:755632888
1905 1905 c, t dbSNP:773070290
1914 1914 g, t dbSNP:574442434
1932 1932 a, g dbSNP:186510658
1945 1945 a, g dbSNP:553748886
1959 1959 a, c dbSNP:11540657
1962 1962 c, t dbSNP:770655370
1966 1966 c, g dbSNP:758446727
1975 1975 a, g dbSNP:191261480
1978 1978 a, c dbSNP:14538
1992 1992 a, g dbSNP:545708663
1998 1998 a, c dbSNP:74022018
2039 2039 c, g dbSNP:576168372
2042 2042 g, t dbSNP:571144374
2045 2045 a, g dbSNP:541916514
2070 2070 c, t dbSNP:180838974
2072 2072 a, g dbSNP:138560168
2079 2079 c, t dbSNP:548081932
2083 2083 a, g dbSNP:767501618
2099 2099 a, g dbSNP:199531879
2101 2101 a, g dbSNP:77444537

Target ORF information:

RefSeq Version XM_011521783
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011521783

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAAGCTGG AGAGGACCAA CTTGGAGGCC TTGCAGAAGA AGCTGGAGGA GCTAGAGCTT 
GATGAGCAGC AGCGAAAGCG CCTTGAGGCC TTTCTTACCC AGAAGCAGAA GGTGGGAGAA
CTGAAGGATG ACGACTTTGA GAAGATCAGT GAGCTGGGGG CTGGCAATGG CGGTGTGGTG
TTCAAGGTCT CCCACAAGCC TTCTGGCCTG GTCATGGCCA GAAAGCTAAT TCATCTGGAG
ATCAAACCCG CAATCCGGAA CCAGATCATA AGGGAGCTGC AGGTTCTGCA TGAGTGCAAC
TCTCCGTACA TCGTGGGCTT CTATGGTGCG TTCTACAGCG ATGGCGAGAT CAGTATCTGC
ATGGAGCACA TGGATGGAGG TTCTCTGGAT CAAGTCCTGA AGAAAGCTGG AAGAATTCCT
GAACAAATTT TAGGAAAAGT TAGCATTGCT GTAATAAAAG GCCTGACATA TCTGAGGGAG
AAGCACAAGA TCATGCACAG AGATGTCAAG CCCTCCAACA TCCTAGTCAA CTCCCGTGGG
GAGATCAAGC TCTGTGACTT TGGGGTCAGC GGGCAGCTCA TCGACTCCAT GGCCAACTCC
TTCGTGGGCA CAAGGTCCTA CATGTCGCCA GAAAGACTCC AGGGGACTCA TTACTCTGTG
CAGTCAGACA TCTGGAGCAT GGGACTGTCT CTGGTAGAGA TGGCGGTTGG GAGGTATCCC
ATCCCTCCTC CAGATGCCAA GGAGCTGGAG CTGATGTTTG GGTGCCAGGT GGAAGGAGAT
GCGGCTGAGA CCCCACCCAG GCCAAGGACC CCCGGGAGGC CCCTTAGCTC ATACGGAATG
GACAGCCGAC CTCCCATGGC AATTTTTGAG TTGTTGGATT ACATAGTCAA CGAGCCTCCT
CCAAAACTGC CCAGTGGAGT GTTCAGTCTG GAATTTCAAG ATTTTGTGAA TAAATGCTTA
ATAAAAAACC CCGCAGAGAG AGCAGATTTG AAGCAACTCA TGGTTCATGC TTTTATCAAG
AGATCTGATG CTGAGGAAGT GGATTTTGCA GGTTGGCTCT GCTCCACCAT CGGCCTTAAC
CAGCCCAGCA CACCAACCCA TGCTGCTGGC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27418
Clone ID Related Accession (Same CDS sequence) NM_002755
Accession Version NM_002755.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dual specificity mitogen-activated protein kinase kinase 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA653983.1, AK291500.1, AK312356.1, L11284.1, BG709050.1 and CA419665.1. This sequence is a reference standard in the RefSeqGene project. On Mar 12, 2008 this sequence version replaced gi:14589898. Summary: The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L11284.1, AK291500.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCCCAAGA AGAAGCCGAC GCCCATCCAG CTGAACCCGG CCCCCGACGG CTCTGCAGTT 
AACGGGACCA GCTCTGCGGA GACCAACTTG GAGGCCTTGC AGAAGAAGCT GGAGGAGCTA
GAGCTTGATG AGCAGCAGCG AAAGCGCCTT GAGGCCTTTC TTACCCAGAA GCAGAAGGTG
GGAGAACTGA AGGATGACGA CTTTGAGAAG ATCAGTGAGC TGGGGGCTGG CAATGGCGGT
GTGGTGTTCA AGGTCTCCCA CAAGCCTTCT GGCCTGGTCA TGGCCAGAAA GCTAATTCAT
CTGGAGATCA AACCCGCAAT CCGGAACCAG ATCATAAGGG AGCTGCAGGT TCTGCATGAG
TGCAACTCTC CGTACATCGT GGGCTTCTAT GGTGCGTTCT ACAGCGATGG CGAGATCAGT
ATCTGCATGG AGCACATGGA TGGAGGTTCT CTGGATCAAG TCCTGAAGAA AGCTGGAAGA
ATTCCTGAAC AAATTTTAGG AAAAGTTAGC ATTGCTGTAA TAAAAGGCCT GACATATCTG
AGGGAGAAGC ACAAGATCAT GCACAGAGAT GTCAAGCCCT CCAACATCCT AGTCAACTCC
CGTGGGGAGA TCAAGCTCTG TGACTTTGGG GTCAGCGGGC AGCTCATCGA CTCCATGGCC
AACTCCTTCG TGGGCACAAG GTCCTACATG TCGCCAGAAA GACTCCAGGG GACTCATTAC
TCTGTGCAGT CAGACATCTG GAGCATGGGA CTGTCTCTGG TAGAGATGGC GGTTGGGAGG
TATCCCATCC CTCCTCCAGA TGCCAAGGAG CTGGAGCTGA TGTTTGGGTG CCAGGTGGAA
GGAGATGCGG CTGAGACCCC ACCCAGGCCA AGGACCCCCG GGAGGCCCCT TAGCTCATAC
GGAATGGACA GCCGACCTCC CATGGCAATT TTTGAGTTGT TGGATTACAT AGTCAACGAG
CCTCCTCCAA AACTGCCCAG TGGAGTGTTC AGTCTGGAAT TTCAAGATTT TGTGAATAAA
TGCTTAATAA AAAACCCCGC AGAGAGAGCA GATTTGAAGC AACTCATGGT TCATGCTTTT
ATCAAGAGAT CTGATGCTGA GGAAGTGGAT TTTGCAGGTT GGCTCTGCTC CACCATCGGC
CTTAACCAGC CCAGCACACC AACCCATGCT GCTGGCGTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002746.1
CDS476..1657
Misc Feature(1)350..352(+)
Misc Feature(2)497..502(+)
Misc Feature(3)659..1615(+)
Misc Feature(4)677..1558(+)
Misc Feature(5)695..1267(+)
Misc Feature(6)695..1099(+)
Misc Feature(7)704..1267(+)
Misc Feature(8)704..1132(+)
Misc Feature(9)704..709(+)
Misc Feature(10)716..1432(+)
Misc Feature(11)905..913(+)
Misc Feature(12)1094..1159(+)
Misc Feature(13)1097..1111(+)
Misc Feature(14)1127..1129(+)
Misc Feature(15)1127..1129(+)
Misc Feature(16)1127..1129(+)
Misc Feature(17)1127..1129(+)
Misc Feature(18)1127..1129(+)
Misc Feature(19)1136..1429(+)
Misc Feature(20)1139..1141(+)
Misc Feature(21)1139..1141(+)
Misc Feature(22)1139..1141(+)
Misc Feature(23)1139..1141(+)
Misc Feature(24)1139..1141(+)
Misc Feature(25)1283..1396(+)
Misc Feature(26)1331..1333(+)
Misc Feature(27)1331..1333(+)
Misc Feature(28)1331..1333(+)
Misc Feature(29)1349..1351(+)
Misc Feature(30)1349..1351(+)
Misc Feature(31)1367..1369(+)
Misc Feature(32)1367..1369(+)
Misc Feature(33)1385..1387(+)
Misc Feature(34)1631..1633(+)
Exon (1)1..555
Gene:MAP2K1
Gene Synonym:
Exon (2)556..766
Gene:MAP2K1
Gene Synonym:
Exon (3)767..913
Gene:MAP2K1
Gene Synonym:
Exon (4)914..991
Gene:MAP2K1
Gene Synonym:
Exon (5)992..1043
Gene:MAP2K1
Gene Synonym:
Exon (6)1044..1168
Gene:MAP2K1
Gene Synonym:
Exon (7)1169..1370
Gene:MAP2K1
Gene Synonym:
Exon (8)1371..1435
Gene:MAP2K1
Gene Synonym:
Exon (9)1436..1497
Gene:MAP2K1
Gene Synonym:
Exon (10)1498..1543
Gene:MAP2K1
Gene Synonym:
Exon (11)1544..2586
Gene:MAP2K1
Gene Synonym:
Translation
Position Chain Variation Link
24 24 c, t dbSNP:776124833
28 28 c, t dbSNP:73469977
49 49 c, t dbSNP:185210930
68 68 g, t dbSNP:536414539
85 85 c, t dbSNP:140766704
152 152 c, g dbSNP:573326843
169 169 -, a dbSNP:548883212
212 212 g, t dbSNP:545081204
254 254 -, gc dbSNP:141005341
258 258 c, t dbSNP:558949572
271 271 c, t dbSNP:575529090
276 276 a, g dbSNP:544924354
278 278 c, g dbSNP:561213249
307 307 a, g dbSNP:530252769
318 318 a, g dbSNP:369406498
333 333 c, g dbSNP:111281925
345 345 c, t dbSNP:540384307
355 355 g, t dbSNP:559034006
356 356 a, c dbSNP:534948137
364 364 -, ggcccgc dbSNP:575854200
391 391 c, g dbSNP:112542693
396 396 a, g dbSNP:41277718
432 432 a, g dbSNP:551186099
433 433 c, t dbSNP:571248158
438 438 c, t dbSNP:768750195
439 439 -, c dbSNP:142027715
442 442 a, c dbSNP:530407397
444 444 c, g dbSNP:761617161
445 445 -, c dbSNP:730880340
459 459 c, g dbSNP:769505606
469 469 a, g dbSNP:772853727
474 474 a, g dbSNP:77796976
481 481 c, g, t dbSNP:377720622
486 486 a, g dbSNP:761150136
519 519 c, t dbSNP:764535990
531 531 c, g dbSNP:727504413
538 538 c, t dbSNP:567535653
544 544 c, t dbSNP:140749690
549 549 c, t dbSNP:553162958
562 562 c, t dbSNP:755038798
571 571 c, t dbSNP:781144142
593 593 -, c dbSNP:759503971
599 599 c, t dbSNP:397516789
601 601 c, t dbSNP:748100663
622 622 a, c dbSNP:756073029
631 631 c, t dbSNP:147489724
633 633 c, t dbSNP:121908594
637 637 g, t dbSNP:748988504
640 640 c, g dbSNP:770743929
644 644 a, c dbSNP:397516790
652 652 a, g dbSNP:773928828
654 654 g, t dbSNP:730880501
673 673 c, t dbSNP:56200325
674 674 a, g dbSNP:727504317
690 690 a, g dbSNP:547530008
712 712 c, t dbSNP:148656020
726 726 a, g dbSNP:774932586
738 738 a, g dbSNP:762354445
739 739 a, g dbSNP:7494832
750 750 g, t dbSNP:397516791
751 751 c, g dbSNP:377323150
752 752 a, g dbSNP:151207265
754 754 c, t dbSNP:140387057
755 755 a, g dbSNP:368143400
756 756 g, t dbSNP:762110054
761 761 a, g dbSNP:751770209
783 783 g, t dbSNP:730880502
790 790 c, t dbSNP:144166521
791 791 a, g dbSNP:752994941
798 798 a, g dbSNP:727504819
808 808 c, t dbSNP:764248591
830 830 c, t dbSNP:730880503
832 832 c, t dbSNP:753763967
840 840 a, g dbSNP:757254963
843 843 c, g dbSNP:778650731
846 846 c, t dbSNP:397516792
847 847 a, g dbSNP:750270430
853 853 c, t dbSNP:369401783
858 858 gc, tt dbSNP:730880508
858 858 g, t dbSNP:121908596
863 863 c, t dbSNP:397516793
864 864 a, g dbSNP:121908595
871 871 a, g, t dbSNP:139364105
880 880 c, t dbSNP:767989355
886 886 a, c, t dbSNP:146415623
887 887 a, g dbSNP:730880504
902 902 a, g dbSNP:533695080
909 909 a, g dbSNP:771604017
914 914 c, g dbSNP:755707394
916 916 c, t dbSNP:779497093
928 928 -, g dbSNP:755398652
941 941 a, g dbSNP:199504372
944 944 a, g dbSNP:201208360
946 946 a, g dbSNP:200487483
952 952 a, c dbSNP:772312180
967 967 a, g dbSNP:140822439
970 970 g, t dbSNP:747171317
991 991 c, t dbSNP:768918234
1024 1024 a, g dbSNP:747352922
1029 1029 a, g dbSNP:769044712
1040 1040 a, c dbSNP:776763061
1067 1067 c, g dbSNP:774278448
1076 1076 c, t dbSNP:759605592
1081 1081 a, g dbSNP:771990100
1083 1083 a, g dbSNP:727503996
1090 1090 a, g dbSNP:775467166
1099 1099 c, t dbSNP:191996882
1102 1102 c, t dbSNP:144639340
1106 1106 a, g dbSNP:727505206
1111 1111 c, t dbSNP:138466751
1123 1123 c, t dbSNP:148968935
1124 1124 a, g dbSNP:761324501
1132 1132 c, g dbSNP:730880506
1186 1186 a, g dbSNP:17586159
1199 1199 a, g dbSNP:761435303
1201 1201 g, t dbSNP:373745627
1204 1204 a, g dbSNP:772752167
1205 1205 g, t dbSNP:730880507
1238 1238 c, g dbSNP:760016123
1246 1246 a, g dbSNP:767965126
1247 1247 a, g dbSNP:753236280
1249 1249 c, t dbSNP:756434943
1267 1267 g, t dbSNP:764379969
1276 1276 a, t dbSNP:754086223
1278 1278 c, t dbSNP:757459909
1279 1279 c, g dbSNP:371652992
1285 1285 a, g dbSNP:745891583
1309 1309 a, g dbSNP:148151221
1319 1319 a, g dbSNP:780066304
1323 1323 c, t dbSNP:144080051
1337 1337 c, t dbSNP:768480988
1339 1339 c, t dbSNP:776361123
1346 1346 a, g dbSNP:747807884
1349 1349 a, g dbSNP:769383345
1350 1350 c, g dbSNP:397516794
1354 1354 c, t dbSNP:370521459
1357 1357 g, t dbSNP:762411863
1375 1375 c, t dbSNP:147333830
1376 1376 a, g dbSNP:773890369
1391 1391 c, t dbSNP:761222249
1402 1402 a, t dbSNP:146869577
1407 1407 c, t dbSNP:777224813
1432 1432 c, t dbSNP:143019052
1435 1435 -, g dbSNP:756917248
1436 1436 c, t dbSNP:771613524
1444 1444 a, c dbSNP:776941883
1445 1445 a, c dbSNP:762436311
1453 1453 a, c dbSNP:538987451
1459 1459 a, t dbSNP:773755873
1462 1462 a, g, t dbSNP:763249437
1472 1472 c, g dbSNP:749645202
1475 1475 g, t dbSNP:751840710
1481 1481 c, g dbSNP:754765552
1483 1483 g, t dbSNP:767650713
1494 1494 a, c dbSNP:184970289
1504 1504 -, aaaa dbSNP:775355010
1509 1509 a, g dbSNP:760766158
1513 1513 c, t dbSNP:547178339
1514 1514 a, g dbSNP:746354996
1526 1526 a, g, t dbSNP:373210982
1537 1537 a, c dbSNP:750035241
1544 1544 g, t dbSNP:558326525
1573 1573 c, t dbSNP:200293968
1576 1576 g, t dbSNP:770847542
1582 1582 a, g dbSNP:537811569
1584 1584 a, c dbSNP:759243614
1588 1588 c, t dbSNP:767237502
1598 1598 a, c, t dbSNP:752538915
1605 1605 c, t dbSNP:371140798
1610 1610 a, g dbSNP:373794940
1612 1612 c, t dbSNP:150841154
1613 1613 a, g dbSNP:750934083
1614 1614 a, g dbSNP:28730804
1618 1618 a, t dbSNP:758866695
1638 1638 c, t dbSNP:780370635
1642 1642 c, t dbSNP:751868729
1643 1643 a, g dbSNP:730880500
1651 1651 c, t dbSNP:781328871
1652 1652 a, g dbSNP:200442489
1656 1656 a, c dbSNP:756208132
1674 1674 a, t dbSNP:777783461
1680 1680 c, t dbSNP:376181437
1683 1683 c, g dbSNP:749220939
1687 1687 c, t dbSNP:771041748
1688 1688 c, t dbSNP:774452900
1693 1693 c, g, t dbSNP:574828384
1694 1694 a, g dbSNP:775193561
1697 1697 a, g dbSNP:540533521
1699 1699 c, t dbSNP:763625326
1702 1702 a, g dbSNP:776051290
1705 1705 a, g dbSNP:761330415
1707 1707 a, g dbSNP:766828156
1709 1709 a, c, t dbSNP:372452427
1710 1710 a, g dbSNP:755282867
1715 1715 -, g dbSNP:756795713
1720 1720 c, t dbSNP:767930298
1721 1721 g, t dbSNP:752962298
1723 1723 c, t dbSNP:756331255
1729 1729 a, g dbSNP:369901206
1736 1736 g, t dbSNP:777889018
1738 1738 g, t dbSNP:749280724
1739 1739 c, t dbSNP:576922968
1743 1743 g, t dbSNP:757416394
1748 1748 c, t dbSNP:778934345
1753 1753 a, g dbSNP:757367010
1758 1758 -, c dbSNP:778371173
1784 1784 a, g dbSNP:771988141
1805 1805 -, tt dbSNP:745540522
1813 1813 c, t dbSNP:373707256
1910 1910 c, t dbSNP:546295504
1916 1916 a, c dbSNP:181822467
1944 1944 a, g dbSNP:184860087
1965 1965 a, c dbSNP:14303
1996 1996 g, t dbSNP:371021779
2000 2000 c, t dbSNP:372877813
2015 2015 a, g dbSNP:561736994
2046 2046 a, c dbSNP:528023985
2057 2057 c, t dbSNP:772667633
2061 2061 c, t dbSNP:190094372
2082 2082 a, g, t dbSNP:56366941
2113 2113 c, t dbSNP:181853123
2187 2187 c, t dbSNP:552268563
2199 2199 c, g dbSNP:747416216
2247 2247 a, g dbSNP:568999981
2249 2249 a, g dbSNP:147974693
2301 2301 a, g dbSNP:375853830
2314 2314 a, g dbSNP:8684
2347 2347 -, tt dbSNP:570295657
2349 2349 c, t dbSNP:755632888
2380 2380 c, t dbSNP:773070290
2389 2389 g, t dbSNP:574442434
2407 2407 a, g dbSNP:186510658
2420 2420 a, g dbSNP:553748886
2434 2434 a, c dbSNP:11540657
2437 2437 c, t dbSNP:770655370
2441 2441 c, g dbSNP:758446727
2450 2450 a, g dbSNP:191261480
2453 2453 a, c dbSNP:14538
2467 2467 a, g dbSNP:545708663
2473 2473 a, c dbSNP:74022018
2514 2514 c, g dbSNP:576168372
2517 2517 g, t dbSNP:571144374
2520 2520 a, g dbSNP:541916514
2545 2545 c, t dbSNP:180838974
2547 2547 a, g dbSNP:138560168
2554 2554 c, t dbSNP:548081932
2558 2558 a, g dbSNP:767501618
2574 2574 a, g dbSNP:199531879
2576 2576 a, g dbSNP:77444537

Target ORF information:

RefSeq Version NM_002755
Organism Homo sapiens (human)
Definition Homo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002755

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCCCAAGA AGAAGCCGAC GCCCATCCAG CTGAACCCGG CCCCCGACGG CTCTGCAGTT 
AACGGGACCA GCTCTGCGGA GACCAACTTG GAGGCCTTGC AGAAGAAGCT GGAGGAGCTA
GAGCTTGATG AGCAGCAGCG AAAGCGCCTT GAGGCCTTTC TTACCCAGAA GCAGAAGGTG
GGAGAACTGA AGGATGACGA CTTTGAGAAG ATCAGTGAGC TGGGGGCTGG CAATGGCGGT
GTGGTGTTCA AGGTCTCCCA CAAGCCTTCT GGCCTGGTCA TGGCCAGAAA GCTAATTCAT
CTGGAGATCA AACCCGCAAT CCGGAACCAG ATCATAAGGG AGCTGCAGGT TCTGCATGAG
TGCAACTCTC CGTACATCGT GGGCTTCTAT GGTGCGTTCT ACAGCGATGG CGAGATCAGT
ATCTGCATGG AGCACATGGA TGGAGGTTCT CTGGATCAAG TCCTGAAGAA AGCTGGAAGA
ATTCCTGAAC AAATTTTAGG AAAAGTTAGC ATTGCTGTAA TAAAAGGCCT GACATATCTG
AGGGAGAAGC ACAAGATCAT GCACAGAGAT GTCAAGCCCT CCAACATCCT AGTCAACTCC
CGTGGGGAGA TCAAGCTCTG TGACTTTGGG GTCAGCGGGC AGCTCATCGA CTCCATGGCC
AACTCCTTCG TGGGCACAAG GTCCTACATG TCGCCAGAAA GACTCCAGGG GACTCATTAC
TCTGTGCAGT CAGACATCTG GAGCATGGGA CTGTCTCTGG TAGAGATGGC GGTTGGGAGG
TATCCCATCC CTCCTCCAGA TGCCAAGGAG CTGGAGCTGA TGTTTGGGTG CCAGGTGGAA
GGAGATGCGG CTGAGACCCC ACCCAGGCCA AGGACCCCCG GGAGGCCCCT TAGCTCATAC
GGAATGGACA GCCGACCTCC CATGGCAATT TTTGAGTTGT TGGATTACAT AGTCAACGAG
CCTCCTCCAA AACTGCCCAG TGGAGTGTTC AGTCTGGAAT TTCAAGATTT TGTGAATAAA
TGCTTAATAA AAAACCCCGC AGAGAGAGCA GATTTGAAGC AACTCATGGT TCATGCTTTT
ATCAAGAGAT CTGATGCTGA GGAAGTGGAT TTTGCAGGTT GGCTCTGCTC CACCATCGGC
CTTAACCAGC CCAGCACACC AACCCATGCT GCTGGCGTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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