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PRPS1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol PRPS1
Entrez Gene ID 5631
Full Name phosphoribosyl pyrophosphate synthetase 1
Synonyms ARTS, CMTX5, DFN2, DFNX1, PPRibP, PRS-I, PRSI
General protein information
Preferred Names
ribose-phosphate pyrophosphokinase 1
Names
ribose-phosphate pyrophosphokinase 1
deafness 2, perceptive, congenital
ribose-phosphate diphosphokinase 1
phosphoribosyl pyrophosphate synthase I
deafness, X-linked 2, perceptive, congenital
dJ1070B1.2 (phosphoribosyl pyrophosphate synthetase 1)
NP_001191331.1
NP_002755.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xq22.3

Summary This gene encodes an enzyme that catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for purine metabolism and nucleotide biosynthesis. Defects in this gene are a cause of phosphoribosylpyrophosphate synthetase superactivity, Charcot-Marie-Tooth disease X-linked recessive type 5 and Arts Syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]. lac of sum
Disorder MIM:

311850

Disorder Html: Gout, PRPS-related, 300661 (3); Phosphoribosylpyrophosphate

mRNA and Protein(s)

mRNA Protein Name
NM_002764 NP_002755 ribose-phosphate pyrophosphokinase 1 isoform 1
NM_001204402 NP_001191331 ribose-phosphate pyrophosphokinase 1 isoform 2



Homo sapiens (human) PRPS1 NP_002755.1
Pan troglodytes (chimpanzee) PRPS1 XP_001144504.1
Macaca mulatta (Rhesus monkey) PRPS1 XP_001095604.1
Canis lupus familiaris (dog) PRPS1 XP_005641702.1
Bos taurus (cattle) PRPS1 NP_001039654.1
Mus musculus (house mouse) Prps1 NP_067438.1
Rattus norvegicus (Norway rat) Prps1 NP_058939.1
Gallus gallus (chicken) PRPS1 XP_004940671.1
Danio rerio (zebrafish) prps1b NP_001070036.1
Drosophila melanogaster (fruit fly) CG6767 NP_729528.2
Arabidopsis thaliana (thale cress) AT2G35390 NP_850244.1
Arabidopsis thaliana (thale cress) PRS2 NP_174516.1
Arabidopsis thaliana (thale cress) AT2G44530 NP_181981.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PRPS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRPS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu26049
NM_002764 Homo sapiens phosphoribosyl pyrophosphate synthetase 1 (PRPS1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu21900
NM_001204402 Homo sapiens phosphoribosyl pyrophosphate synthetase 1 (PRPS1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu26049
Clone ID Related Accession (Same CDS sequence) NM_002764
Accession Version NM_002764.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 957bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ribose-phosphate pyrophosphokinase 1 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL137787.11 and BC001605.1. This sequence is a reference standard in the RefSeqGene project. On Jan 12, 2008 this sequence version replaced gi:19923737. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D00860.1, AK223169.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCGAATA TCAAAATCTT CAGCGGCAGC TCCCACCAGG ACTTATCTCA GAAAATTGCT 
GACCGCCTGG GCCTGGAGCT AGGCAAGGTG GTGACTAAGA AATTCAGCAA CCAGGAGACC
TGTGTGGAAA TTGGTGAAAG TGTACGTGGA GAGGATGTCT ACATTGTTCA GAGTGGTTGT
GGCGAAATCA ATGACAATTT AATGGAGCTT TTGATCATGA TTAATGCCTG CAAGATTGCT
TCAGCCAGCC GGGTTACTGC AGTCATCCCA TGCTTCCCTT ATGCCCGGCA GGATAAGAAA
GATAAGAGCC GGGCGCCAAT CTCAGCCAAG CTTGTTGCAA ATATGCTATC TGTAGCAGGT
GCAGATCATA TTATCACCAT GGACCTACAT GCTTCTCAAA TTCAGGGCTT TTTTGATATC
CCAGTAGACA ATTTGTATGC AGAGCCGGCT GTCCTAAAGT GGATAAGGGA GAATATCTCT
GAGTGGAGGA ACTGCACTAT TGTCTCACCT GATGCTGGTG GAGCTAAGAG AGTGACCTCC
ATTGCAGACA GGCTGAATGT GGACTTTGCC TTGATTCACA AAGAACGGAA GAAGGCCAAT
GAAGTGGACC GCATGGTGCT TGTGGGAGAT GTGAAGGATC GGGTGGCCAT CCTTGTGGAT
GACATGGCTG ACACTTGTGG CACAATCTGC CATGCAGCTG ACAAACTTCT CTCAGCTGGC
GCCACCAGAG TTTATGCCAT CTTGACTCAT GGAATCTTCT CCGGTCCTGC TATTTCTCGC
ATCAACAACG CATGCTTTGA GGCAGTAGTA GTCACCAATA CCATACCTCA GGAGGACAAG
ATGAAGCATT GCTCCAAAAT ACAGGTGATT GACATCTCTA TGATCCTTGC AGAAGCCATC
AGGAGAACTC ACAATGGAGA ATCCGTTTCT TACCTATTCA GCCATGTCCC TTTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002755.1
CDS206..1162
Misc Feature(1)194..196(+)
Misc Feature(2)206..1144(+)
Misc Feature(3)215..565(+)
Misc Feature(4)644..1027(+)
Misc Feature(5)716..961(+)
Misc Feature(6)839..886(+)
Exon (1)1..327
Gene:PRPS1
Gene Synonym:
Exon (2)328..511
Gene:PRPS1
Gene Synonym:
Exon (3)512..610
Gene:PRPS1
Gene Synonym:
Exon (4)611..735
Gene:PRPS1
Gene Synonym:
Exon (5)736..909
Gene:PRPS1
Gene Synonym:
Exon (6)910..1069
Gene:PRPS1
Gene Synonym:
Exon (7)1070..2156
Gene:PRPS1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002764
Organism Homo sapiens (human)
Definition Homo sapiens phosphoribosyl pyrophosphate synthetase 1 (PRPS1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002764

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCGAATA TCAAAATCTT CAGCGGCAGC TCCCACCAGG ACTTATCTCA GAAAATTGCT 
GACCGCCTGG GCCTGGAGCT AGGCAAGGTG GTGACTAAGA AATTCAGCAA CCAGGAGACC
TGTGTGGAAA TTGGTGAAAG TGTACGTGGA GAGGATGTCT ACATTGTTCA GAGTGGTTGT
GGCGAAATCA ATGACAATTT AATGGAGCTT TTGATCATGA TTAATGCCTG CAAGATTGCT
TCAGCCAGCC GGGTTACTGC AGTCATCCCA TGCTTCCCTT ATGCCCGGCA GGATAAGAAA
GATAAGAGCC GGGCGCCAAT CTCAGCCAAG CTTGTTGCAA ATATGCTATC TGTAGCAGGT
GCAGATCATA TTATCACCAT GGACCTACAT GCTTCTCAAA TTCAGGGCTT TTTTGATATC
CCAGTAGACA ATTTGTATGC AGAGCCGGCT GTCCTAAAGT GGATAAGGGA GAATATCTCT
GAGTGGAGGA ACTGCACTAT TGTCTCACCT GATGCTGGTG GAGCTAAGAG AGTGACCTCC
ATTGCAGACA GGCTGAATGT GGACTTTGCC TTGATTCACA AAGAACGGAA GAAGGCCAAT
GAAGTGGACC GCATGGTGCT TGTGGGAGAT GTGAAGGATC GGGTGGCCAT CCTTGTGGAT
GACATGGCTG ACACTTGTGG CACAATCTGC CATGCAGCTG ACAAACTTCT CTCAGCTGGC
GCCACCAGAG TTTATGCCAT CTTGACTCAT GGAATCTTCT CCGGTCCTGC TATTTCTCGC
ATCAACAACG CATGCTTTGA GGCAGTAGTA GTCACCAATA CCATACCTCA GGAGGACAAG
ATGAAGCATT GCTCCAAAAT ACAGGTGATT GACATCTCTA TGATCCTTGC AGAAGCCATC
AGGAGAACTC ACAATGGAGA ATCCGTTTCT TACCTATTCA GCCATGTCCC TTTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21900
Clone ID Related Accession (Same CDS sequence) NM_001204402
Accession Version NM_001204402.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 345bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ribose-phosphate pyrophosphokinase 1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL137787.11, DC411129.1, AK316467.1 and D00860.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK316467.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGGTGCTTG TGGGAGATGT GAAGGATCGG GTGGCCATCC TTGTGGATGA CATGGCTGAC 
ACTTGTGGCA CAATCTGCCA TGCAGCTGAC AAACTTCTCT CAGCTGGCGC CACCAGAGTT
TATGCCATCT TGACTCATGG AATCTTCTCC GGTCCTGCTA TTTCTCGCAT CAACAACGCA
TGCTTTGAGG CAGTAGTAGT CACCAATACC ATACCTCAGG AGGACAAGAT GAAGCATTGC
TCCAAAATAC AGGTGATTGA CATCTCTATG ATCCTTGCAG AAGCCATCAG GAGAACTCAC
AATGGAGAAT CCGTTTCTTA CCTATTCAGC CATGTCCCTT TATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191331.1
CDS410..754
Misc Feature(1)326..328(+)
Misc Feature(2)410..619(+)
Misc Feature(3)455..553(+)
Exon (1)1..327
Gene:PRPS1
Gene Synonym:
Exon (2)328..501
Gene:PRPS1
Gene Synonym:
Exon (3)502..661
Gene:PRPS1
Gene Synonym:
Exon (4)662..1748
Gene:PRPS1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001204402
Organism Homo sapiens (human)
Definition Homo sapiens phosphoribosyl pyrophosphate synthetase 1 (PRPS1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204402

ORF Insert Sequence:

1
61
121
181
241
301
ATGGTGCTTG TGGGAGATGT GAAGGATCGG GTGGCCATCC TTGTGGATGA CATGGCTGAC 
ACTTGTGGCA CAATCTGCCA TGCAGCTGAC AAACTTCTCT CAGCTGGCGC CACCAGAGTT
TATGCCATCT TGACTCATGG AATCTTCTCC GGTCCTGCTA TTTCTCGCAT CAACAACGCA
TGCTTTGAGG CAGTAGTAGT CACCAATACC ATACCTCAGG AGGACAAGAT GAAGCATTGC
TCCAAAATAC AGGTGATTGA CATCTCTATG ATCCTTGCAG AAGCCATCAG GAGAACTCAC
AATGGAGAAT CCGTTTCTTA CCTATTCAGC CATGTCCCTT TATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A small disturbance, but a serious disease: the possible mechanism of D52H-mutant of human PRS1 that causes gout
IUBMB Life 65 (6), 518-525 (2013)
Chen P, Li J, Ma J, Teng M and Li X.


book

Hearing loss and PRPS1 mutations: Wide spectrum of phenotypes and potential therapy
Int J Audiol 52 (1), 23-28 (2013)
Liu XZ, Xie D, Yuan HJ, de Brouwer AP, Christodoulou J and Yan D.


book

DFNX1 Nonsyndromic Hearing Loss and Deafness
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Yuan,H. and Liu,X.Z.


book

Arts Syndrome
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
de Brouwer,A.P.M., Duley,J.A. and Christodoulou,J.


book

Phosphoribosylpyrophosphate Synthetase Superactivity
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
De Brouwer,A.P.M., Duley,J.A. and Christodoulou,J.


book

Charcot-Marie-Tooth Neuropathy X Type 5
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Kim,J.W. and Kim,H.J.


book

Deafness and Hereditary Hearing Loss Overview
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Smith,R.J.H., Shearer,A.E., Hildebrand,M.S. and Van Camp,G.


book

Charcot-Marie-Tooth Hereditary Neuropathy Overview
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Bird,T.D.


book

Promoter regions of the human X-linked housekeeping genes PRPS1 and PRPS2 encoding phosphoribosylpyrophosphate synthetase subunit I and II isoforms
Biochim. Biophys. Acta 1130 (2), 139-148 (1992)
Ishizuka T, Iizasa T, Taira M, Ishijima S, Sonoda T, Shimada H, Nagatake N and Tatibana M.


book

Complete nucleotide sequence of human phosphoribosyl pyrophosphate synthetase subunit I (PRS I) cDNA and a comparison with human and rat PRPS gene families
J. Biochem. 109 (2), 361-364 (1991)
Sonoda T, Taira M, Ishijima S, Ishizuka T, Iizasa T and Tatibana M.


 
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