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C10orf2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol C10orf2
Entrez Gene ID 56652
Full Name chromosome 10 open reading frame 2
Synonyms ATXN8, IOSCA, MTDPS7, PEO, PEO1, PEOA3, PRLTS5, SANDO, SCA8, TWINL
General protein information
Preferred Names
twinkle protein, mitochondrial
Names
twinkle protein, mitochondrial
ataxin 8
mitochondrial twinkle protein
T7-like mitochondrial DNA helicase
progressive external ophthalmoplegia 1 protein
T7 gp4-like protein with intramitochondrial nucleoid localization
T7 helicase-related protein with intramitochondrial nucleoid localization
NP_001157284.1
NP_001157285.1
NP_001157286.1
NP_068602.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

10

10q24

Summary This gene encodes a hexameric DNA helicase which unwinds short stretches of double-stranded DNA in the 5' to 3' direction and, along with mitochondrial single-stranded DNA binding protein and mtDNA polymerase gamma, is thought to play a key role in mtDNA replication. The protein localizes to the mitochondrial matrix and mitochondrial nucleoids. Mutations in this gene cause infantile onset spinocerebellar ataxia (IOSCA) and progressive external ophthalmoplegia (PEO) and are also associated with several mitochondrial depletion syndromes. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Aug 2009]. lac of sum
Disorder MIM:

606075

Disorder Html: Progressive external ophthalmoplegia, autosomal dominant, 3, 609286

mRNA and Protein(s)

mRNA Protein Name
XM_011539974 XP_011538276 twinkle protein, mitochondrial isoform X1
XM_011539975 XP_011538277 twinkle protein, mitochondrial isoform X1
NM_001163814 NP_001157286 twinkle protein, mitochondrial isoform D
NM_021830 NP_068602 twinkle protein, mitochondrial isoform A
NM_001163812 NP_001157284 twinkle protein, mitochondrial isoform B
NM_001163813 NP_001157285 twinkle protein, mitochondrial isoform C



Homo sapiens (human) C10orf2 NP_068602.2
Pan troglodytes (chimpanzee) C10H10orf2 XP_003951780.1
Macaca mulatta (Rhesus monkey) C9H10orf2 XP_001109287.1
Canis lupus familiaris (dog) C28H10orf2 XP_543974.1
Bos taurus (cattle) C26H10orf2 NP_001091933.1
Mus musculus (house mouse) Peo1 NP_722491.2
Rattus norvegicus (Norway rat) Peo1 NP_001101069.1
Gallus gallus (chicken) PEO1 NP_001026515.1
Danio rerio (zebrafish) peo1 NP_001264527.1
Drosophila melanogaster (fruit fly) CG5924 NP_609318.1
Caenorhabditis elegans F46G11.1 NP_508973.3
Xenopus (Silurana) tropicalis (western clawed frog) LOC100491229 XP_002938766.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following C10orf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the C10orf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu26432 XM_011539974 PREDICTED: Homo sapiens chromosome 10 open reading frame 2 (C10orf2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $139.30
$199.00
OHu26432 XM_011539975 PREDICTED: Homo sapiens chromosome 10 open reading frame 2 (C10orf2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $139.30
$199.00
OHu22794 NM_001163814 Homo sapiens chromosome 10 open reading frame 2 (C10orf2), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu26782
NM_021830 Homo sapiens chromosome 10 open reading frame 2 (C10orf2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$344.50-$482.30
$689.00
OHu26964 NM_001163812 Homo sapiens chromosome 10 open reading frame 2 (C10orf2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00
OHu26432 NM_001163813 Homo sapiens chromosome 10 open reading frame 2 (C10orf2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $139.30
$199.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu26432
Clone ID Related Accession (Same CDS sequence) NM_001163813 , XM_011539974 , XM_011539975
Accession Version XM_011539974.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 693bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product twinkle protein, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG
GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA
GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC
AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT
GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC
GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011538276.1
CDS151..843
Misc Feature(1)151..684(+)
Misc Feature(2)322..336(+)
Translation

Target ORF information:

RefSeq Version XM_011539974
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens chromosome 10 open reading frame 2 (C10orf2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011539974

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG
GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA
GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC
AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT
GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC
GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26432
Clone ID Related Accession (Same CDS sequence) NM_001163813 , XM_011539974 , XM_011539975
Accession Version XM_011539975.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 693bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product twinkle protein, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG
GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA
GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC
AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT
GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC
GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011538277.1
CDS105..797
Misc Feature(1)105..638(+)
Misc Feature(2)276..290(+)
Translation

Target ORF information:

RefSeq Version XM_011539975
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens chromosome 10 open reading frame 2 (C10orf2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011539975

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG
GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA
GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC
AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT
GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC
GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22794
Clone ID Related Accession (Same CDS sequence) NM_001163814
Accession Version NM_001163814.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 387bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product twinkle protein, mitochondrial isoform D
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112401.1, AL133215.17 and AK025485.1. Transcript Variant: This variant (4) has multiple splice site differences, compared to variant 1. The encoded protein (isoform D) lacks most of the N-terminus, which contains a mitochondrial targeting sequence and probable ssDNA binding domain, and has a shorter and distinct C-terminus, which lacks a C-terminal domain required for hexamer formation, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AL529767.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
GGCTCAGCCA AAGTGAGTGG CCTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001157286.1
CDS198..584
Misc Feature(1)198..>575(+)
Misc Feature(2)369..383(+)
Exon (1)1..78
Gene:C10orf2
Gene Synonym:
Exon (2)79..319
Gene:C10orf2
Gene Synonym:
Exon (3)320..427
Gene:C10orf2
Gene Synonym:
Exon (4)428..613
Gene:C10orf2
Gene Synonym:
Exon (5)614..1825
Gene:C10orf2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001163814
Organism Homo sapiens (human)
Definition Homo sapiens chromosome 10 open reading frame 2 (C10orf2), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001163814

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
GGCTCAGCCA AAGTGAGTGG CCTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26782
Clone ID Related Accession (Same CDS sequence) NM_021830
Accession Version NM_021830.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2055bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product twinkle protein, mitochondrial isoform A
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112401.1, DB090322.1, AF292004.1 and AK025485.1. This sequence is a reference standard in the RefSeqGene project. On Aug 5, 2009 this sequence version replaced gi:39725941. Transcript Variant: This variant (1) encodes the longest isoform (A; also known as Twinkle). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC033762.1, AF292004.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGTGGGTCC TCCTCCGAAG TGGGTACCCC CTCCGTATCT TGTTACCCCT GCGTGGGGAG 
TGGATGGGTC GGAGGGGCCT GCCCCGAAAC TTGGCCCCAG GCCCTCCTCG CAGACGTTAC
AGGAAGGAGA CTCTCCAAGC CTTGGATATG CCAGTGTTGC CTGTAACTGC AACTGAAATC
CGCCAGTATT TGCGGGGGCA TGGGATCCCC TTCCAGGATG GTCACAGTTG CCTGCGGGCA
CTGAGCCCCT TTGCAGAGTC TTCACAGCTC AAAGGCCAGA CTGGTGTTAC CACTTCCTTC
AGCCTCTTCA TTGACAAGAC CACAGGCCAC TTTCTCTGCA TGACCAGCCT AGCAGAAGGG
AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCCAG GGAGGGGTTT
CTGCTTAGCA AGGCACCAGA ATTTGAGGAC AGCGAGGAGG TCCGGAGGAT CTGGAACCGA
GCAATACCTC TCTGGGAGCT GCCTGATCAG GAGGAGGTTC AGCTGGCTGA TACAATGTTT
GGCCTTACCA AGGTTACAGA TGACACACTC AAGCGTTTCA GTGTGCGATA TCTGCGACCT
GCTCGCAGTC TTGTCTTCCC TTGGTTCTCC CCTGGGGGCT CAGGATTACG AGGCCTGAAG
CTCCTAGAGG CTAAATGCCA GGGGGATGGA GTGAGCTACG AGGAAACCAC TATTCCCCGA
CCCAGCGCCT ACCACAATCT GTTTGGATTA CCACTGATTA GTCGTCGAGA TGCTGAGGTG
GTACTGACGA GTCGTGAGCT TGACAGCCTG GCCTTGAACC AGTCCACGGG GCTGCCTACC
CTTACTCTAC CCCGAGGAAC GACCTGCTTA CCCCCTGCCT TACTCCCTTA CCTGGAACAG
TTCCGGCGGA TTGTATTCTG GTTGGGGGAT GACCTTCGGT CCTGGGAAGC CGCCAAGTTG
TTTGCACGAA AACTGAACCC CAAACGATGC TTCTTGGTGC GACCAGGAGA CCAGCAACCC
CGTCCCCTGG AGGCCCTGAA CGGAGGCTTC AATCTTTCTC GTATTCTTCG TACCGCCCTG
CCTGCCTGGC ACAAGTCCAT CGTATCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGAGAA
CTGTCAAATG TGGAGCAAGC AGCTGGCCTC CGCTGGAGCC GCTTTCCAGA CCTCAATCGT
ATCTTGAAGG GACATCGAAA GGGCGAGCTG ACGGTCTTCA CAGGGCCAAC AGGCAGTGGA
AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTT CCCAGGGGGT GAACACACTG
TGGGGTAGCT TCGAGATCAG CAATGTGAGA CTAGCCCGGG TCATGCTGAC ACAGTTTGCC
GAGGGGCGGC TGGAAGATCA ACTGGACAAA TATGATCACT GGGCTGACCG CTTTGAGGAC
CTGCCCCTCT ATTTCATGAC TTTCCATGGA CAGCAAAGCA TCAGGACTGT AATAGATACA
ATGCAACATG CAGTCTACGT CTATGACATT TGTCATGTGA TCATCGACAA CCTGCAGTTC
ATGATGGGTC ACGAGCAGCT GTCCACAGAC AGGATCGCAG CTCAAGACTA CATCATCGGG
GTCTTTCGGA AGTTTGCAAC AGACAATAAC TGCCATGTGA CACTGGTCAT TCACCCCCGG
AAAGAGGATG ATGACAAGGA ACTGCAGACA GCGTCCATTT TTGGCTCAGC CAAAGCAAGC
CAGGAAGCAG ACAATGTTCT GATCCTGCAG GACAGGAAGC TGGTAACCGG GCCAGGGAAA
CGGTATCTGC AGGTGTCCAA GAACCGCTTT GATGGAGATG TAGGTGTCTT CCCGCTTGAG
TTCAACAAGA ACTCCCTCAC CTTCTCCATT CCACCAAAGA ACAAGGCCCG GCTCAAGAAG
ATCAAGGATG ACACTGGACC AGTGGCCAAA AAGCCCTCTT CTGGCAAAAA GGGGGCTACG
ACACAGAACT CTGAGATTTG CTCAGGCCAG GCCCCCACTC CCGACCAGCC AGACACCTCC
AAGCGTTCAA AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_068602.2
CDS676..2730
Misc Feature(1)613..615(+)
Misc Feature(2)1450..1680(+)
Misc Feature(3)1465..1614(+)
Misc Feature(4)1465..1602(+)
Misc Feature(5)1471..1530(+)
Misc Feature(6)1795..2571(+)
Misc Feature(7)1921..1941(+)
Misc Feature(8)1936..2448(+)
Misc Feature(9)2212..2223(+)
Misc Feature(10)2236..2472(+)
Exon (1)1..1918
Gene:C10orf2
Gene Synonym:
Exon (2)1919..2159
Gene:C10orf2
Gene Synonym:
Exon (3)2160..2267
Gene:C10orf2
Gene Synonym:
Exon (4)2268..2409
Gene:C10orf2
Gene Synonym:
Exon (5)2410..3621
Gene:C10orf2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_021830
Organism Homo sapiens (human)
Definition Homo sapiens chromosome 10 open reading frame 2 (C10orf2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_021830

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGTGGGTCC TCCTCCGAAG TGGGTACCCC CTCCGTATCT TGTTACCCCT GCGTGGGGAG 
TGGATGGGTC GGAGGGGCCT GCCCCGAAAC TTGGCCCCAG GCCCTCCTCG CAGACGTTAC
AGGAAGGAGA CTCTCCAAGC CTTGGATATG CCAGTGTTGC CTGTAACTGC AACTGAAATC
CGCCAGTATT TGCGGGGGCA TGGGATCCCC TTCCAGGATG GTCACAGTTG CCTGCGGGCA
CTGAGCCCCT TTGCAGAGTC TTCACAGCTC AAAGGCCAGA CTGGTGTTAC CACTTCCTTC
AGCCTCTTCA TTGACAAGAC CACAGGCCAC TTTCTCTGCA TGACCAGCCT AGCAGAAGGG
AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCCAG GGAGGGGTTT
CTGCTTAGCA AGGCACCAGA ATTTGAGGAC AGCGAGGAGG TCCGGAGGAT CTGGAACCGA
GCAATACCTC TCTGGGAGCT GCCTGATCAG GAGGAGGTTC AGCTGGCTGA TACAATGTTT
GGCCTTACCA AGGTTACAGA TGACACACTC AAGCGTTTCA GTGTGCGATA TCTGCGACCT
GCTCGCAGTC TTGTCTTCCC TTGGTTCTCC CCTGGGGGCT CAGGATTACG AGGCCTGAAG
CTCCTAGAGG CTAAATGCCA GGGGGATGGA GTGAGCTACG AGGAAACCAC TATTCCCCGA
CCCAGCGCCT ACCACAATCT GTTTGGATTA CCACTGATTA GTCGTCGAGA TGCTGAGGTG
GTACTGACGA GTCGTGAGCT TGACAGCCTG GCCTTGAACC AGTCCACGGG GCTGCCTACC
CTTACTCTAC CCCGAGGAAC GACCTGCTTA CCCCCTGCCT TACTCCCTTA CCTGGAACAG
TTCCGGCGGA TTGTATTCTG GTTGGGGGAT GACCTTCGGT CCTGGGAAGC CGCCAAGTTG
TTTGCACGAA AACTGAACCC CAAACGATGC TTCTTGGTGC GACCAGGAGA CCAGCAACCC
CGTCCCCTGG AGGCCCTGAA CGGAGGCTTC AATCTTTCTC GTATTCTTCG TACCGCCCTG
CCTGCCTGGC ACAAGTCCAT CGTATCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGAGAA
CTGTCAAATG TGGAGCAAGC AGCTGGCCTC CGCTGGAGCC GCTTTCCAGA CCTCAATCGT
ATCTTGAAGG GACATCGAAA GGGCGAGCTG ACGGTCTTCA CAGGGCCAAC AGGCAGTGGA
AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTT CCCAGGGGGT GAACACACTG
TGGGGTAGCT TCGAGATCAG CAATGTGAGA CTAGCCCGGG TCATGCTGAC ACAGTTTGCC
GAGGGGCGGC TGGAAGATCA ACTGGACAAA TATGATCACT GGGCTGACCG CTTTGAGGAC
CTGCCCCTCT ATTTCATGAC TTTCCATGGA CAGCAAAGCA TCAGGACTGT AATAGATACA
ATGCAACATG CAGTCTACGT CTATGACATT TGTCATGTGA TCATCGACAA CCTGCAGTTC
ATGATGGGTC ACGAGCAGCT GTCCACAGAC AGGATCGCAG CTCAAGACTA CATCATCGGG
GTCTTTCGGA AGTTTGCAAC AGACAATAAC TGCCATGTGA CACTGGTCAT TCACCCCCGG
AAAGAGGATG ATGACAAGGA ACTGCAGACA GCGTCCATTT TTGGCTCAGC CAAAGCAAGC
CAGGAAGCAG ACAATGTTCT GATCCTGCAG GACAGGAAGC TGGTAACCGG GCCAGGGAAA
CGGTATCTGC AGGTGTCCAA GAACCGCTTT GATGGAGATG TAGGTGTCTT CCCGCTTGAG
TTCAACAAGA ACTCCCTCAC CTTCTCCATT CCACCAAAGA ACAAGGCCCG GCTCAAGAAG
ATCAAGGATG ACACTGGACC AGTGGCCAAA AAGCCCTCTT CTGGCAAAAA GGGGGCTACG
ACACAGAACT CTGAGATTTG CTCAGGCCAG GCCCCCACTC CCGACCAGCC AGACACCTCC
AAGCGTTCAA AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26964
Clone ID Related Accession (Same CDS sequence) NM_001163812
Accession Version NM_001163812.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1749bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product twinkle protein, mitochondrial isoform B
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112401.1, DB090322.1, AF292005.1, BC013349.2 and AK025485.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region which introduces a novel stop codon, compared to variant 1. The encoded protein (isoform B; also known as Twinky) has a shorter and distinct C-terminus, which lacks a C-terminal domain required for hexamer formation, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC013349.2, AF292005.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGTGGGTCC TCCTCCGAAG TGGGTACCCC CTCCGTATCT TGTTACCCCT GCGTGGGGAG 
TGGATGGGTC GGAGGGGCCT GCCCCGAAAC TTGGCCCCAG GCCCTCCTCG CAGACGTTAC
AGGAAGGAGA CTCTCCAAGC CTTGGATATG CCAGTGTTGC CTGTAACTGC AACTGAAATC
CGCCAGTATT TGCGGGGGCA TGGGATCCCC TTCCAGGATG GTCACAGTTG CCTGCGGGCA
CTGAGCCCCT TTGCAGAGTC TTCACAGCTC AAAGGCCAGA CTGGTGTTAC CACTTCCTTC
AGCCTCTTCA TTGACAAGAC CACAGGCCAC TTTCTCTGCA TGACCAGCCT AGCAGAAGGG
AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCCAG GGAGGGGTTT
CTGCTTAGCA AGGCACCAGA ATTTGAGGAC AGCGAGGAGG TCCGGAGGAT CTGGAACCGA
GCAATACCTC TCTGGGAGCT GCCTGATCAG GAGGAGGTTC AGCTGGCTGA TACAATGTTT
GGCCTTACCA AGGTTACAGA TGACACACTC AAGCGTTTCA GTGTGCGATA TCTGCGACCT
GCTCGCAGTC TTGTCTTCCC TTGGTTCTCC CCTGGGGGCT CAGGATTACG AGGCCTGAAG
CTCCTAGAGG CTAAATGCCA GGGGGATGGA GTGAGCTACG AGGAAACCAC TATTCCCCGA
CCCAGCGCCT ACCACAATCT GTTTGGATTA CCACTGATTA GTCGTCGAGA TGCTGAGGTG
GTACTGACGA GTCGTGAGCT TGACAGCCTG GCCTTGAACC AGTCCACGGG GCTGCCTACC
CTTACTCTAC CCCGAGGAAC GACCTGCTTA CCCCCTGCCT TACTCCCTTA CCTGGAACAG
TTCCGGCGGA TTGTATTCTG GTTGGGGGAT GACCTTCGGT CCTGGGAAGC CGCCAAGTTG
TTTGCACGAA AACTGAACCC CAAACGATGC TTCTTGGTGC GACCAGGAGA CCAGCAACCC
CGTCCCCTGG AGGCCCTGAA CGGAGGCTTC AATCTTTCTC GTATTCTTCG TACCGCCCTG
CCTGCCTGGC ACAAGTCCAT CGTATCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGAGAA
CTGTCAAATG TGGAGCAAGC AGCTGGCCTC CGCTGGAGCC GCTTTCCAGA CCTCAATCGT
ATCTTGAAGG GACATCGAAA GGGCGAGCTG ACGGTCTTCA CAGGGCCAAC AGGCAGTGGA
AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTT CCCAGGGGGT GAACACACTG
TGGGGTAGCT TCGAGATCAG CAATGTGAGA CTAGCCCGGG TCATGCTGAC ACAGTTTGCC
GAGGGGCGGC TGGAAGATCA ACTGGACAAA TATGATCACT GGGCTGACCG CTTTGAGGAC
CTGCCCCTCT ATTTCATGAC TTTCCATGGA CAGCAAAGCA TCAGGACTGT AATAGATACA
ATGCAACATG CAGTCTACGT CTATGACATT TGTCATGTGA TCATCGACAA CCTGCAGTTC
ATGATGGGTC ACGAGCAGCT GTCCACAGAC AGGATCGCAG CTCAAGACTA CATCATCGGG
GTCTTTCGGA AGTTTGCAAC AGACAATAAC TGCCATGTGA CACTGGTCAT TCACCCCCGG
AAAGAGGATG ATGACAAGGA ACTGCAGACA GCGTCCATTT TTGGCTCAGC CAAAGTGAGT
GGCCTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001157284.1
CDS676..2424
Misc Feature(1)613..615(+)
Misc Feature(2)1450..1680(+)
Misc Feature(3)1465..1614(+)
Misc Feature(4)1465..1602(+)
Misc Feature(5)1471..1530(+)
Misc Feature(6)1795..>2415(+)
Misc Feature(7)1918..1941(+)
Misc Feature(8)1921..2226(+)
Misc Feature(9)2209..2223(+)
Exon (1)1..1918
Gene:C10orf2
Gene Synonym:
Exon (2)1919..2159
Gene:C10orf2
Gene Synonym:
Exon (3)2160..2267
Gene:C10orf2
Gene Synonym:
Exon (4)2268..2453
Gene:C10orf2
Gene Synonym:
Exon (5)2454..3665
Gene:C10orf2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001163812
Organism Homo sapiens (human)
Definition Homo sapiens chromosome 10 open reading frame 2 (C10orf2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001163812

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGTGGGTCC TCCTCCGAAG TGGGTACCCC CTCCGTATCT TGTTACCCCT GCGTGGGGAG 
TGGATGGGTC GGAGGGGCCT GCCCCGAAAC TTGGCCCCAG GCCCTCCTCG CAGACGTTAC
AGGAAGGAGA CTCTCCAAGC CTTGGATATG CCAGTGTTGC CTGTAACTGC AACTGAAATC
CGCCAGTATT TGCGGGGGCA TGGGATCCCC TTCCAGGATG GTCACAGTTG CCTGCGGGCA
CTGAGCCCCT TTGCAGAGTC TTCACAGCTC AAAGGCCAGA CTGGTGTTAC CACTTCCTTC
AGCCTCTTCA TTGACAAGAC CACAGGCCAC TTTCTCTGCA TGACCAGCCT AGCAGAAGGG
AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCCAG GGAGGGGTTT
CTGCTTAGCA AGGCACCAGA ATTTGAGGAC AGCGAGGAGG TCCGGAGGAT CTGGAACCGA
GCAATACCTC TCTGGGAGCT GCCTGATCAG GAGGAGGTTC AGCTGGCTGA TACAATGTTT
GGCCTTACCA AGGTTACAGA TGACACACTC AAGCGTTTCA GTGTGCGATA TCTGCGACCT
GCTCGCAGTC TTGTCTTCCC TTGGTTCTCC CCTGGGGGCT CAGGATTACG AGGCCTGAAG
CTCCTAGAGG CTAAATGCCA GGGGGATGGA GTGAGCTACG AGGAAACCAC TATTCCCCGA
CCCAGCGCCT ACCACAATCT GTTTGGATTA CCACTGATTA GTCGTCGAGA TGCTGAGGTG
GTACTGACGA GTCGTGAGCT TGACAGCCTG GCCTTGAACC AGTCCACGGG GCTGCCTACC
CTTACTCTAC CCCGAGGAAC GACCTGCTTA CCCCCTGCCT TACTCCCTTA CCTGGAACAG
TTCCGGCGGA TTGTATTCTG GTTGGGGGAT GACCTTCGGT CCTGGGAAGC CGCCAAGTTG
TTTGCACGAA AACTGAACCC CAAACGATGC TTCTTGGTGC GACCAGGAGA CCAGCAACCC
CGTCCCCTGG AGGCCCTGAA CGGAGGCTTC AATCTTTCTC GTATTCTTCG TACCGCCCTG
CCTGCCTGGC ACAAGTCCAT CGTATCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGAGAA
CTGTCAAATG TGGAGCAAGC AGCTGGCCTC CGCTGGAGCC GCTTTCCAGA CCTCAATCGT
ATCTTGAAGG GACATCGAAA GGGCGAGCTG ACGGTCTTCA CAGGGCCAAC AGGCAGTGGA
AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTT CCCAGGGGGT GAACACACTG
TGGGGTAGCT TCGAGATCAG CAATGTGAGA CTAGCCCGGG TCATGCTGAC ACAGTTTGCC
GAGGGGCGGC TGGAAGATCA ACTGGACAAA TATGATCACT GGGCTGACCG CTTTGAGGAC
CTGCCCCTCT ATTTCATGAC TTTCCATGGA CAGCAAAGCA TCAGGACTGT AATAGATACA
ATGCAACATG CAGTCTACGT CTATGACATT TGTCATGTGA TCATCGACAA CCTGCAGTTC
ATGATGGGTC ACGAGCAGCT GTCCACAGAC AGGATCGCAG CTCAAGACTA CATCATCGGG
GTCTTTCGGA AGTTTGCAAC AGACAATAAC TGCCATGTGA CACTGGTCAT TCACCCCCGG
AAAGAGGATG ATGACAAGGA ACTGCAGACA GCGTCCATTT TTGGCTCAGC CAAAGTGAGT
GGCCTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26432
Clone ID Related Accession (Same CDS sequence) NM_001163813 , XM_011539974 , XM_011539975
Accession Version NM_001163813.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 693bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product twinkle protein, mitochondrial isoform C
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112401.1, BQ643363.1 and AK025485.1. Transcript Variant: This variant (3) uses an alternate splice site in exon 1 that results in the use of an in-frame downstream start codon, compared to variant 1. The encoded protein (isoform C) lacks most of the N-terminus, which contains a mitochondrial targeting sequence and probable ssDNA binding domain, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK297068.1, BQ643363.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG
GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA
GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC
AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT
GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC
GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001157285.1
CDS198..890
Misc Feature(1)198..731(+)
Misc Feature(2)369..383(+)
Exon (1)1..78
Gene:C10orf2
Gene Synonym:
Exon (2)79..319
Gene:C10orf2
Gene Synonym:
Exon (3)320..427
Gene:C10orf2
Gene Synonym:
Exon (4)428..569
Gene:C10orf2
Gene Synonym:
Exon (5)570..1781
Gene:C10orf2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001163813
Organism Homo sapiens (human)
Definition Homo sapiens chromosome 10 open reading frame 2 (C10orf2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001163813

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG
GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA
GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC
AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT
GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC
GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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