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PTEN cDNA ORF clone, Homo sapiens (human)

Gene Symbol PTEN
Entrez Gene ID 5728
Full Name phosphatase and tensin homolog
Synonyms 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, TEP1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

10

10q23.3

Summary This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2015]. lac of sum
Disorder MIM:

601728

Disorder Html: Cowden disease, 158350 (3); Lhermitte-Duclos syndrome, 158350 (3); Bannayan-Riley-Ruvalcaba syndrome, 153480 (3); {Meningioma}, 607174 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_001304717 NP_001291646 phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform PTEN-L
XM_006717926 XP_006717989 phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform X1
XM_011539981 XP_011538283 phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform X2
XM_011539982 XP_011538284 phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform X3
NM_001304718 NP_001291647 phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform 3
NM_000314 NP_000305 phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform PTEN



Homo sapiens (human) PTEN NP_000305.3
Pan troglodytes (chimpanzee) PTEN XP_521544.3
Macaca mulatta (Rhesus monkey) PTEN XP_001102542.1
Canis lupus familiaris (dog) PTEN NP_001003192.1
Bos taurus (cattle) PTEN XP_002698416.1
Mus musculus (house mouse) Pten NP_032986.1
Rattus norvegicus (Norway rat) Pten NP_113794.1
Gallus gallus (chicken) PTEN XP_421555.2
Danio rerio (zebrafish) ptenb NP_001001822.1
Drosophila melanogaster (fruit fly) Pten NP_001162933.1
Xenopus (Silurana) tropicalis (western clawed frog) pten NP_001116943.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PTEN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTEN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu59999 NM_001304717 Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $307.30
$439.00
OHu44441 XM_006717926 PREDICTED: Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu60000 XM_011539981 PREDICTED: Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu60001 XM_011539982 PREDICTED: Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu60002 NM_001304718 Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $139.30
$199.00
OHu27709
NM_000314 Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu59999
Clone ID Related Accession (Same CDS sequence) NM_001304717
Accession Version NM_001304717.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1731bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 10-JUL-2015
Organism Homo sapiens (human)
Product phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform PTEN-L
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U92436.1, AC063965.8, BC005821.2 and AA836562.1. On Apr 1, 2015 this sequence version replaced gi:754502061. Transcript Variant: This variant (1) encodes multiple isoforms due to the use of alternative translation initiation codons. The longest isoform (PTEN-L, PMID:23744781; also known as PTENalpha, PMID: 24768297) is derived from the use of an upstream non-AUG (CUG) start codon, while two shorter isoforms are derived from downstream AUG start codons. PTEN-L, initiates with a Leucine, rather than a Methionine, is thought to be preferentially associated with the mitochondrial inner membrane (PMID: 24768297), and is represented in this RefSeq. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U92436.1, BC005821.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 23744781, 24768297 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
CTGGAGCGGG GGGGAGAAGC GGCGGCGGCG GCGGCCGCGG CGGCTGCAGC TCCAGGGAGG 
GGGTCTGAGT CGCCTGTCAC CATTTCCAGG GCTGGGAACG CCGGAGAGTT GGTCTCTCCC
CTTCTACTGC CTCCAACACG GCGGCGGCGG CGGCGGCACA TCCAGGGACC CGGGCCGGTT
TTAAACCTCC CGTCCGCCGC CGCCGCACCC CCCGTGGCCC GGGCTCCGGA GGCCGCCGGC
GGAGGCAGCC GTTCGGAGGA TTATTCGTCT TCTCCCCATT CCGCTGCCGC CGCTGCCAGG
CCTCTGGCTG CTGAGGAGAA GCAGGCCCAG TCGCTGCAAC CATCCAGCAG CCGCCGCAGC
AGCCATTACC CGGCTGCGGT CCAGAGCCAA GCGGCGGCAG AGCGAGGGGC ATCAGCTACC
GCCAAGTCCA GAGCCATTTC CATCCTGCAG AAGAAGCCCC GCCACCAGCA GCTTCTGCCA
TCTCTCTCCT CCTTTTTCTT CAGCCACAGG CTCCCAGACA TGACAGCCAT CATCAAAGAG
ATCGTTAGCA GAAACAAAAG GAGATATCAA GAGGATGGAT TCGACTTAGA CTTGACCTAT
ATTTATCCAA ACATTATTGC TATGGGATTT CCTGCAGAAA GACTTGAAGG CGTATACAGG
AACAATATTG ATGATGTAGT AAGGTTTTTG GATTCAAAGC ATAAAAACCA TTACAAGATA
TACAATCTTT GTGCTGAAAG ACATTATGAC ACCGCCAAAT TTAATTGCAG AGTTGCACAA
TATCCTTTTG AAGACCATAA CCCACCACAG CTAGAACTTA TCAAACCCTT TTGTGAAGAT
CTTGACCAAT GGCTAAGTGA AGATGACAAT CATGTTGCAG CAATTCACTG TAAAGCTGGA
AAGGGACGAA CTGGTGTAAT GATATGTGCA TATTTATTAC ATCGGGGCAA ATTTTTAAAG
GCACAAGAGG CCCTAGATTT CTATGGGGAA GTAAGGACCA GAGACAAAAA GGGAGTAACT
ATTCCCAGTC AGAGGCGCTA TGTGTATTAT TATAGCTACC TGTTAAAGAA TCATCTGGAT
TATAGACCAG TGGCACTGTT GTTTCACAAG ATGATGTTTG AAACTATTCC AATGTTCAGT
GGCGGAACTT GCAATCCTCA GTTTGTGGTC TGCCAGCTAA AGGTGAAGAT ATATTCCTCC
AATTCAGGAC CCACACGACG GGAAGACAAG TTCATGTACT TTGAGTTCCC TCAGCCGTTA
CCTGTGTGTG GTGATATCAA AGTAGAGTTC TTCCACAAAC AGAACAAGAT GCTAAAAAAG
GACAAAATGT TTCACTTTTG GGTAAATACA TTCTTCATAC CAGGACCAGA GGAAACCTCA
GAAAAAGTAG AAAATGGAAG TCTATGTGAT CAAGAAATCG ATAGCATTTG CAGTATAGAG
CGTGCAGATA ATGACAAGGA ATATCTAGTA CTTACTTTAA CAAAAAATGA TCTTGACAAA
GCAAATAAAG ACAAAGCCAA CCGATACTTT TCTCCAAATT TTAAGGTGAA GCTGTACTTC
ACAAAAACAG TAGAGGAGCC GTCAAATCCA GAGGCTAGCA GTTCAACTTC TGTAACACCA
GATGTTAGTG ACAATGAACC TGATCATTAT AGATATTCTG ACACCACTGA CTCTGATCCA
GAGAATGAAC CTTTTGATGA AGATCAGCAT ACACAAATTA CAAAAGTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001291646.2
CDS513..2243
Misc Feature(1)318..320(+)
Misc Feature(2)1032..1034(+)
Misc Feature(3)1305..1580(+)
Misc Feature(4)1305..1424(+)
Misc Feature(5)1593..2078(+)
Exon (1)1..1110
Gene:PTEN
Gene Synonym:
Exon (2)1111..1195
Gene:PTEN
Gene Synonym:
Exon (3)1196..1240
Gene:PTEN
Gene Synonym:
Exon (4)1241..1284
Gene:PTEN
Gene Synonym:
Exon (5)1285..1523
Gene:PTEN
Gene Synonym:
Exon (6)1524..1665
Gene:PTEN
Gene Synonym:
Exon (7)1666..1832
Gene:PTEN
Gene Synonym:
Exon (8)1833..2057
Gene:PTEN
Gene Synonym:
Exon (9)2058..8701
Gene:PTEN
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001304717
Organism Homo sapiens (human)
Definition Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001304717

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
CTGGAGCGGG GGGGAGAAGC GGCGGCGGCG GCGGCCGCGG CGGCTGCAGC TCCAGGGAGG 
GGGTCTGAGT CGCCTGTCAC CATTTCCAGG GCTGGGAACG CCGGAGAGTT GGTCTCTCCC
CTTCTACTGC CTCCAACACG GCGGCGGCGG CGGCGGCACA TCCAGGGACC CGGGCCGGTT
TTAAACCTCC CGTCCGCCGC CGCCGCACCC CCCGTGGCCC GGGCTCCGGA GGCCGCCGGC
GGAGGCAGCC GTTCGGAGGA TTATTCGTCT TCTCCCCATT CCGCTGCCGC CGCTGCCAGG
CCTCTGGCTG CTGAGGAGAA GCAGGCCCAG TCGCTGCAAC CATCCAGCAG CCGCCGCAGC
AGCCATTACC CGGCTGCGGT CCAGAGCCAA GCGGCGGCAG AGCGAGGGGC ATCAGCTACC
GCCAAGTCCA GAGCCATTTC CATCCTGCAG AAGAAGCCCC GCCACCAGCA GCTTCTGCCA
TCTCTCTCCT CCTTTTTCTT CAGCCACAGG CTCCCAGACA TGACAGCCAT CATCAAAGAG
ATCGTTAGCA GAAACAAAAG GAGATATCAA GAGGATGGAT TCGACTTAGA CTTGACCTAT
ATTTATCCAA ACATTATTGC TATGGGATTT CCTGCAGAAA GACTTGAAGG CGTATACAGG
AACAATATTG ATGATGTAGT AAGGTTTTTG GATTCAAAGC ATAAAAACCA TTACAAGATA
TACAATCTTT GTGCTGAAAG ACATTATGAC ACCGCCAAAT TTAATTGCAG AGTTGCACAA
TATCCTTTTG AAGACCATAA CCCACCACAG CTAGAACTTA TCAAACCCTT TTGTGAAGAT
CTTGACCAAT GGCTAAGTGA AGATGACAAT CATGTTGCAG CAATTCACTG TAAAGCTGGA
AAGGGACGAA CTGGTGTAAT GATATGTGCA TATTTATTAC ATCGGGGCAA ATTTTTAAAG
GCACAAGAGG CCCTAGATTT CTATGGGGAA GTAAGGACCA GAGACAAAAA GGGAGTAACT
ATTCCCAGTC AGAGGCGCTA TGTGTATTAT TATAGCTACC TGTTAAAGAA TCATCTGGAT
TATAGACCAG TGGCACTGTT GTTTCACAAG ATGATGTTTG AAACTATTCC AATGTTCAGT
GGCGGAACTT GCAATCCTCA GTTTGTGGTC TGCCAGCTAA AGGTGAAGAT ATATTCCTCC
AATTCAGGAC CCACACGACG GGAAGACAAG TTCATGTACT TTGAGTTCCC TCAGCCGTTA
CCTGTGTGTG GTGATATCAA AGTAGAGTTC TTCCACAAAC AGAACAAGAT GCTAAAAAAG
GACAAAATGT TTCACTTTTG GGTAAATACA TTCTTCATAC CAGGACCAGA GGAAACCTCA
GAAAAAGTAG AAAATGGAAG TCTATGTGAT CAAGAAATCG ATAGCATTTG CAGTATAGAG
CGTGCAGATA ATGACAAGGA ATATCTAGTA CTTACTTTAA CAAAAAATGA TCTTGACAAA
GCAAATAAAG ACAAAGCCAA CCGATACTTT TCTCCAAATT TTAAGGTGAA GCTGTACTTC
ACAAAAACAG TAGAGGAGCC GTCAAATCCA GAGGCTAGCA GTTCAACTTC TGTAACACCA
GATGTTAGTG ACAATGAACC TGATCATTAT AGATATTCTG ACACCACTGA CTCTGATCCA
GAGAATGAAC CTTTTGATGA AGATCAGCAT ACACAAATTA CAAAAGTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu44441
Clone ID Related Accession (Same CDS sequence) XM_006717926
Accession Version XM_006717926.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1167bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578819652. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACAGCCA TCATCAAAGA GATCGTTAGC AGAAACAAAA GGAGATATCA AGAGGATGGA 
TTCGACTTAG ACTTGACCTA TATTTATCCA AACATTATTG CTATGGGATT TCCTGCAGAA
AGACTTGAAG GCGTATACAG GAACAATATT GATGATGTAG TAAGTTGTGC TGAAAGACAT
TATGACACCG CCAAATTTAA TTGCAGAGTT GCACAATATC CTTTTGAAGA CCATAACCCA
CCACAGCTAG AACTTATCAA ACCCTTTTGT GAAGATCTTG ACCAATGGCT AAGTGAAGAT
GACAATCATG TTGCAGCAAT TCACTGTAAA GCTGGAAAGG GACGAACTGG TGTAATGATA
TGTGCATATT TATTACATCG GGGCAAATTT TTAAAGGCAC AAGAGGCCCT AGATTTCTAT
GGGGAAGTAA GGACCAGAGA CAAAAAGGGA GTAACTATTC CCAGTCAGAG GCGCTATGTG
TATTATTATA GCTACCTGTT AAAGAATCAT CTGGATTATA GACCAGTGGC ACTGTTGTTT
CACAAGATGA TGTTTGAAAC TATTCCAATG TTCAGTGGCG GAACTTGCAA TCCTCAGTTT
GTGGTCTGCC AGCTAAAGGT GAAGATATAT TCCTCCAATT CAGGACCCAC ACGACGGGAA
GACAAGTTCA TGTACTTTGA GTTCCCTCAG CCGTTACCTG TGTGTGGTGA TATCAAAGTA
GAGTTCTTCC ACAAACAGAA CAAGATGCTA AAAAAGGACA AAATGTTTCA CTTTTGGGTA
AATACATTCT TCATACCAGG ACCAGAGGAA ACCTCAGAAA AAGTAGAAAA TGGAAGTCTA
TGTGATCAAG AAATCGATAG CATTTGCAGT ATAGAGCGTG CAGATAATGA CAAGGAATAT
CTAGTACTTA CTTTAACAAA AAATGATCTT GACAAAGCAA ATAAAGACAA AGCCAACCGA
TACTTTTCTC CAAATTTTAA GGTGAAGCTG TACTTCACAA AAACAGTAGA GGAGCCGTCA
AATCCAGAGG CTAGCAGTTC AACTTCTGTA ACACCAGATG TTAGTGACAA TGAACCTGAT
CATTATAGAT ATTCTGACAC CACTGACTCT GATCCAGAGA ATGAACCTTT TGATGAAGAT
CAGCATACAC AAATTACAAA AGTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006717989.1
CDS713..1879
Misc Feature(1)941..1216(+)
Misc Feature(2)941..1060(+)
Misc Feature(3)1229..1714(+)
Translation

Target ORF information:

RefSeq Version XM_006717926
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006717926

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGACAGCCA TCATCAAAGA GATCGTTAGC AGAAACAAAA GGAGATATCA AGAGGATGGA 
TTCGACTTAG ACTTGACCTA TATTTATCCA AACATTATTG CTATGGGATT TCCTGCAGAA
AGACTTGAAG GCGTATACAG GAACAATATT GATGATGTAG TAAGTTGTGC TGAAAGACAT
TATGACACCG CCAAATTTAA TTGCAGAGTT GCACAATATC CTTTTGAAGA CCATAACCCA
CCACAGCTAG AACTTATCAA ACCCTTTTGT GAAGATCTTG ACCAATGGCT AAGTGAAGAT
GACAATCATG TTGCAGCAAT TCACTGTAAA GCTGGAAAGG GACGAACTGG TGTAATGATA
TGTGCATATT TATTACATCG GGGCAAATTT TTAAAGGCAC AAGAGGCCCT AGATTTCTAT
GGGGAAGTAA GGACCAGAGA CAAAAAGGGA GTAACTATTC CCAGTCAGAG GCGCTATGTG
TATTATTATA GCTACCTGTT AAAGAATCAT CTGGATTATA GACCAGTGGC ACTGTTGTTT
CACAAGATGA TGTTTGAAAC TATTCCAATG TTCAGTGGCG GAACTTGCAA TCCTCAGTTT
GTGGTCTGCC AGCTAAAGGT GAAGATATAT TCCTCCAATT CAGGACCCAC ACGACGGGAA
GACAAGTTCA TGTACTTTGA GTTCCCTCAG CCGTTACCTG TGTGTGGTGA TATCAAAGTA
GAGTTCTTCC ACAAACAGAA CAAGATGCTA AAAAAGGACA AAATGTTTCA CTTTTGGGTA
AATACATTCT TCATACCAGG ACCAGAGGAA ACCTCAGAAA AAGTAGAAAA TGGAAGTCTA
TGTGATCAAG AAATCGATAG CATTTGCAGT ATAGAGCGTG CAGATAATGA CAAGGAATAT
CTAGTACTTA CTTTAACAAA AAATGATCTT GACAAAGCAA ATAAAGACAA AGCCAACCGA
TACTTTTCTC CAAATTTTAA GGTGAAGCTG TACTTCACAA AAACAGTAGA GGAGCCGTCA
AATCCAGAGG CTAGCAGTTC AACTTCTGTA ACACCAGATG TTAGTGACAA TGAACCTGAT
CATTATAGAT ATTCTGACAC CACTGACTCT GATCCAGAGA ATGAACCTTT TGATGAAGAT
CAGCATACAC AAATTACAAA AGTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60000
Clone ID Related Accession (Same CDS sequence) XM_011539981
Accession Version XM_011539981.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1035bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGACAGCCA TCATCAAAGA GATCGTTAGC AGAAACAAAA GGAGATATCA AGAGGATGGA 
TTCGACTTAG ACTTGACCTA TATTTATCCA AACATTATTG CTATGGGATT TCCTGCAGAA
AGACTTGAAG GCGTATACAG GAACAATATT GATGATGTAG TAAGGTTTTT GGATTCAAAG
CATAAAAACC ATTACAAGAT ATACAATCTT TGTGCTGAAA GACATTATGA CACCGCCAAA
TTTAATTGCA GAGTTGCACA ATATCCTTTT GAAGACCATA ACCCACCACA GCTAGAACTT
ATCAAACCCT TTTGTGAAGA TCTTGACCAA TGGCTAAGTG AAGATGACAA TCATGTTGCA
GCAATTCACT GTAAAGCTGG AAAGGGACGA ACTGGTGTAA TGATATGTGC ATATTTATTA
CATCGGGGCA AATTTTTAAA GGCACAAGAG GCCCTAGATT TCTATGGGGA AGTAAGGACC
AGAGACAAAA AGGGAGTAAC TATTCCCAGT CAGAGGCGCT ATGTGTATTA TTATAGCTAC
CTGTTAAAGA ATCATCTGGA TTATAGACCA GTGGCACTGT TGTTTCACAA GATGATGTTT
GAAACTATTC CAATGTTCAG TGGCGGAACT TGCAATCCTC AGTTTGTGGT CTGCCAGCTA
AAGGTGAAGA TATATTCCTC CAATTCAGGA CCCACACGAC GGGAAGACAA GTTCATGTAC
TTTGAGTTCC CTCAGCCGTT ACCTGTGTGT GGTGATATCA AAGTAGAGTT CTTCCACAAA
CAGAACAAGA TGCTAAAAAA GGACAAAATG TTTCACTTTT GGGTAAATAC ATTCTTCATA
CCAGGACCAG AGGAAACCTC AGAAAAAGTA GAAAATGGAA GTCTATGTGA TCAAGAAATC
GATAGCATTT GCAGTATAGA GCGTGCAGAT AATGACAAGG AATATCTAGT ACTTACTTTA
ACAAAAAATG ATCTTGACAA AGCAAATAAA GACAAAGCCA ACCGATACTT TTCTCCAAAT
TTTAAGCAAA GATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011538283.1
CDS713..1747
Misc Feature(1)986..1261(+)
Misc Feature(2)986..1105(+)
Misc Feature(3)1274..1738(+)
Translation

Target ORF information:

RefSeq Version XM_011539981
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011539981

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGACAGCCA TCATCAAAGA GATCGTTAGC AGAAACAAAA GGAGATATCA AGAGGATGGA 
TTCGACTTAG ACTTGACCTA TATTTATCCA AACATTATTG CTATGGGATT TCCTGCAGAA
AGACTTGAAG GCGTATACAG GAACAATATT GATGATGTAG TAAGGTTTTT GGATTCAAAG
CATAAAAACC ATTACAAGAT ATACAATCTT TGTGCTGAAA GACATTATGA CACCGCCAAA
TTTAATTGCA GAGTTGCACA ATATCCTTTT GAAGACCATA ACCCACCACA GCTAGAACTT
ATCAAACCCT TTTGTGAAGA TCTTGACCAA TGGCTAAGTG AAGATGACAA TCATGTTGCA
GCAATTCACT GTAAAGCTGG AAAGGGACGA ACTGGTGTAA TGATATGTGC ATATTTATTA
CATCGGGGCA AATTTTTAAA GGCACAAGAG GCCCTAGATT TCTATGGGGA AGTAAGGACC
AGAGACAAAA AGGGAGTAAC TATTCCCAGT CAGAGGCGCT ATGTGTATTA TTATAGCTAC
CTGTTAAAGA ATCATCTGGA TTATAGACCA GTGGCACTGT TGTTTCACAA GATGATGTTT
GAAACTATTC CAATGTTCAG TGGCGGAACT TGCAATCCTC AGTTTGTGGT CTGCCAGCTA
AAGGTGAAGA TATATTCCTC CAATTCAGGA CCCACACGAC GGGAAGACAA GTTCATGTAC
TTTGAGTTCC CTCAGCCGTT ACCTGTGTGT GGTGATATCA AAGTAGAGTT CTTCCACAAA
CAGAACAAGA TGCTAAAAAA GGACAAAATG TTTCACTTTT GGGTAAATAC ATTCTTCATA
CCAGGACCAG AGGAAACCTC AGAAAAAGTA GAAAATGGAA GTCTATGTGA TCAAGAAATC
GATAGCATTT GCAGTATAGA GCGTGCAGAT AATGACAAGG AATATCTAGT ACTTACTTTA
ACAAAAAATG ATCTTGACAA AGCAAATAAA GACAAAGCCA ACCGATACTT TTCTCCAAAT
TTTAAGCAAA GATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60001
Clone ID Related Accession (Same CDS sequence) XM_011539982
Accession Version XM_011539982.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1116bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_030059.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTTACTTT TGAAGAGTGT GATGATTGCA TTTAGGAAGA TATCTAAACT TCTGTTTCAA 
AGCAAAAAGT TTTTGGATTC AAAGCATAAA AACCATTACA AGATATACAA TCTTTGTGCT
GAAAGACATT ATGACACCGC CAAATTTAAT TGCAGAGTTG CACAATATCC TTTTGAAGAC
CATAACCCAC CACAGCTAGA ACTTATCAAA CCCTTTTGTG AAGATCTTGA CCAATGGCTA
AGTGAAGATG ACAATCATGT TGCAGCAATT CACTGTAAAG CTGGAAAGGG ACGAACTGGT
GTAATGATAT GTGCATATTT ATTACATCGG GGCAAATTTT TAAAGGCACA AGAGGCCCTA
GATTTCTATG GGGAAGTAAG GACCAGAGAC AAAAAGGGAG TAACTATTCC CAGTCAGAGG
CGCTATGTGT ATTATTATAG CTACCTGTTA AAGAATCATC TGGATTATAG ACCAGTGGCA
CTGTTGTTTC ACAAGATGAT GTTTGAAACT ATTCCAATGT TCAGTGGCGG AACTTGCAAT
CCTCAGTTTG TGGTCTGCCA GCTAAAGGTG AAGATATATT CCTCCAATTC AGGACCCACA
CGACGGGAAG ACAAGTTCAT GTACTTTGAG TTCCCTCAGC CGTTACCTGT GTGTGGTGAT
ATCAAAGTAG AGTTCTTCCA CAAACAGAAC AAGATGCTAA AAAAGGACAA AATGTTTCAC
TTTTGGGTAA ATACATTCTT CATACCAGGA CCAGAGGAAA CCTCAGAAAA AGTAGAAAAT
GGAAGTCTAT GTGATCAAGA AATCGATAGC ATTTGCAGTA TAGAGCGTGC AGATAATGAC
AAGGAATATC TAGTACTTAC TTTAACAAAA AATGATCTTG ACAAAGCAAA TAAAGACAAA
GCCAACCGAT ACTTTTCTCC AAATTTTAAG GTGAAGCTGT ACTTCACAAA AACAGTAGAG
GAGCCGTCAA ATCCAGAGGC TAGCAGTTCA ACTTCTGTAA CACCAGATGT TAGTGACAAT
GAACCTGATC ATTATAGATA TTCTGACACC ACTGACTCTG ATCCAGAGAA TGAACCTTTT
GATGAAGATC AGCATACACA AATTACAAAA GTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011538284.1
CDS3781..4896
Misc Feature(1)3958..4233(+)
Misc Feature(2)3958..4077(+)
Misc Feature(3)4246..4731(+)
Translation

Target ORF information:

RefSeq Version XM_011539982
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011539982

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTTACTTT TGAAGAGTGT GATGATTGCA TTTAGGAAGA TATCTAAACT TCTGTTTCAA 
AGCAAAAAGT TTTTGGATTC AAAGCATAAA AACCATTACA AGATATACAA TCTTTGTGCT
GAAAGACATT ATGACACCGC CAAATTTAAT TGCAGAGTTG CACAATATCC TTTTGAAGAC
CATAACCCAC CACAGCTAGA ACTTATCAAA CCCTTTTGTG AAGATCTTGA CCAATGGCTA
AGTGAAGATG ACAATCATGT TGCAGCAATT CACTGTAAAG CTGGAAAGGG ACGAACTGGT
GTAATGATAT GTGCATATTT ATTACATCGG GGCAAATTTT TAAAGGCACA AGAGGCCCTA
GATTTCTATG GGGAAGTAAG GACCAGAGAC AAAAAGGGAG TAACTATTCC CAGTCAGAGG
CGCTATGTGT ATTATTATAG CTACCTGTTA AAGAATCATC TGGATTATAG ACCAGTGGCA
CTGTTGTTTC ACAAGATGAT GTTTGAAACT ATTCCAATGT TCAGTGGCGG AACTTGCAAT
CCTCAGTTTG TGGTCTGCCA GCTAAAGGTG AAGATATATT CCTCCAATTC AGGACCCACA
CGACGGGAAG ACAAGTTCAT GTACTTTGAG TTCCCTCAGC CGTTACCTGT GTGTGGTGAT
ATCAAAGTAG AGTTCTTCCA CAAACAGAAC AAGATGCTAA AAAAGGACAA AATGTTTCAC
TTTTGGGTAA ATACATTCTT CATACCAGGA CCAGAGGAAA CCTCAGAAAA AGTAGAAAAT
GGAAGTCTAT GTGATCAAGA AATCGATAGC ATTTGCAGTA TAGAGCGTGC AGATAATGAC
AAGGAATATC TAGTACTTAC TTTAACAAAA AATGATCTTG ACAAAGCAAA TAAAGACAAA
GCCAACCGAT ACTTTTCTCC AAATTTTAAG GTGAAGCTGT ACTTCACAAA AACAGTAGAG
GAGCCGTCAA ATCCAGAGGC TAGCAGTTCA ACTTCTGTAA CACCAGATGT TAGTGACAAT
GAACCTGATC ATTATAGATA TTCTGACACC ACTGACTCTG ATCCAGAGAA TGAACCTTTT
GATGAAGATC AGCATACACA AATTACAAAA GTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60002
Clone ID Related Accession (Same CDS sequence) NM_001304718
Accession Version NM_001304718.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 621bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 26-MAR-2015
Organism Homo sapiens (human)
Product phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U92436.1, AC063965.8, BC005821.2, JF268690.1 and AA836562.1. Transcript Variant: This variant (2) both contains and lacks alternate exons in its 5' UTR compared to variant 1. This variant represents translation initiation at a downstream AUG compared to the more upstream CUG and AUG start codons, as used in variant 1. Use of the more 5' initiation codons, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG to encode an isoform (3) that has a shorter N-terminus, compared to isoform PTENalpha and PTEN. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: JF268690.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGATGTTTG AAACTATTCC AATGTTCAGT GGCGGAACTT GCAATCCTCA GTTTGTGGTC 
TGCCAGCTAA AGGTGAAGAT ATATTCCTCC AATTCAGGAC CCACACGACG GGAAGACAAG
TTCATGTACT TTGAGTTCCC TCAGCCGTTA CCTGTGTGTG GTGATATCAA AGTAGAGTTC
TTCCACAAAC AGAACAAGAT GCTAAAAAAG GACAAAATGT TTCACTTTTG GGTAAATACA
TTCTTCATAC CAGGACCAGA GGAAACCTCA GAAAAAGTAG AAAATGGAAG TCTATGTGAT
CAAGAAATCG ATAGCATTTG CAGTATAGAG CGTGCAGATA ATGACAAGGA ATATCTAGTA
CTTACTTTAA CAAAAAATGA TCTTGACAAA GCAAATAAAG ACAAAGCCAA CCGATACTTT
TCTCCAAATT TTAAGGTGAA GCTGTACTTC ACAAAAACAG TAGAGGAGCC GTCAAATCCA
GAGGCTAGCA GTTCAACTTC TGTAACACCA GATGTTAGTG ACAATGAACC TGATCATTAT
AGATATTCTG ACACCACTGA CTCTGATCCA GAGAATGAAC CTTTTGATGA AGATCAGCAT
ACACAAATTA CAAAAGTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001291647.1
CDS1737..2357
Misc Feature(1)1584..1586(+)
Misc Feature(2)1737..2192(+)
Exon (1)1..1110
Gene:PTEN
Gene Synonym:
Exon (2)1111..1195
Gene:PTEN
Gene Synonym:
Exon (3)1196..1239
Gene:PTEN
Gene Synonym:
Exon (4)1240..1478
Gene:PTEN
Gene Synonym:
Exon (5)1479..1637
Gene:PTEN
Gene Synonym:
Exon (6)1638..1779
Gene:PTEN
Gene Synonym:
Exon (7)1780..1946
Gene:PTEN
Gene Synonym:
Exon (8)1947..2171
Gene:PTEN
Gene Synonym:
Exon (9)2172..8815
Gene:PTEN
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001304718
Organism Homo sapiens (human)
Definition Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001304718

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGATGTTTG AAACTATTCC AATGTTCAGT GGCGGAACTT GCAATCCTCA GTTTGTGGTC 
TGCCAGCTAA AGGTGAAGAT ATATTCCTCC AATTCAGGAC CCACACGACG GGAAGACAAG
TTCATGTACT TTGAGTTCCC TCAGCCGTTA CCTGTGTGTG GTGATATCAA AGTAGAGTTC
TTCCACAAAC AGAACAAGAT GCTAAAAAAG GACAAAATGT TTCACTTTTG GGTAAATACA
TTCTTCATAC CAGGACCAGA GGAAACCTCA GAAAAAGTAG AAAATGGAAG TCTATGTGAT
CAAGAAATCG ATAGCATTTG CAGTATAGAG CGTGCAGATA ATGACAAGGA ATATCTAGTA
CTTACTTTAA CAAAAAATGA TCTTGACAAA GCAAATAAAG ACAAAGCCAA CCGATACTTT
TCTCCAAATT TTAAGGTGAA GCTGTACTTC ACAAAAACAG TAGAGGAGCC GTCAAATCCA
GAGGCTAGCA GTTCAACTTC TGTAACACCA GATGTTAGTG ACAATGAACC TGATCATTAT
AGATATTCTG ACACCACTGA CTCTGATCCA GAGAATGAAC CTTTTGATGA AGATCAGCAT
ACACAAATTA CAAAAGTCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27709
Clone ID Related Accession (Same CDS sequence) NM_000314
Accession Version NM_000314.6 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1212bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-APR-2015
Organism Homo sapiens (human)
Product phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform PTEN
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U92436.1, AC063965.8, BC005821.2 and AA836562.1. On Mar 25, 2015 this sequence version replaced gi:754502060. Transcript Variant: This variant (1) encodes multiple isoforms due to the use of alternative translation initiation codons. The longest isoform (PTEN-L, PMID:23744781; also known as PTENalpha, PMID: 24768297) is derived from the use of an upstream non-AUG (CUG) start codon, while two shorter isoforms are derived from downstream AUG start codons. The most abundant isoform (PTEN), which is derived from the use of 5'-most AUG start codon, is represented in this RefSeq. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:24768297) ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: U92436.1, BC005821.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGACAGCCA TCATCAAAGA GATCGTTAGC AGAAACAAAA GGAGATATCA AGAGGATGGA 
TTCGACTTAG ACTTGACCTA TATTTATCCA AACATTATTG CTATGGGATT TCCTGCAGAA
AGACTTGAAG GCGTATACAG GAACAATATT GATGATGTAG TAAGGTTTTT GGATTCAAAG
CATAAAAACC ATTACAAGAT ATACAATCTT TGTGCTGAAA GACATTATGA CACCGCCAAA
TTTAATTGCA GAGTTGCACA ATATCCTTTT GAAGACCATA ACCCACCACA GCTAGAACTT
ATCAAACCCT TTTGTGAAGA TCTTGACCAA TGGCTAAGTG AAGATGACAA TCATGTTGCA
GCAATTCACT GTAAAGCTGG AAAGGGACGA ACTGGTGTAA TGATATGTGC ATATTTATTA
CATCGGGGCA AATTTTTAAA GGCACAAGAG GCCCTAGATT TCTATGGGGA AGTAAGGACC
AGAGACAAAA AGGGAGTAAC TATTCCCAGT CAGAGGCGCT ATGTGTATTA TTATAGCTAC
CTGTTAAAGA ATCATCTGGA TTATAGACCA GTGGCACTGT TGTTTCACAA GATGATGTTT
GAAACTATTC CAATGTTCAG TGGCGGAACT TGCAATCCTC AGTTTGTGGT CTGCCAGCTA
AAGGTGAAGA TATATTCCTC CAATTCAGGA CCCACACGAC GGGAAGACAA GTTCATGTAC
TTTGAGTTCC CTCAGCCGTT ACCTGTGTGT GGTGATATCA AAGTAGAGTT CTTCCACAAA
CAGAACAAGA TGCTAAAAAA GGACAAAATG TTTCACTTTT GGGTAAATAC ATTCTTCATA
CCAGGACCAG AGGAAACCTC AGAAAAAGTA GAAAATGGAA GTCTATGTGA TCAAGAAATC
GATAGCATTT GCAGTATAGA GCGTGCAGAT AATGACAAGG AATATCTAGT ACTTACTTTA
ACAAAAAATG ATCTTGACAA AGCAAATAAA GACAAAGCCA ACCGATACTT TTCTCCAAAT
TTTAAGGTGA AGCTGTACTT CACAAAAACA GTAGAGGAGC CGTCAAATCC AGAGGCTAGC
AGTTCAACTT CTGTAACACC AGATGTTAGT GACAATGAAC CTGATCATTA TAGATATTCT
GACACCACTG ACTCTGATCC AGAGAATGAA CCTTTTGATG AAGATCAGCA TACACAAATT
ACAAAAGTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000305.3
CDS1032..2243
Misc Feature(1)318..320(+)
Misc Feature(2)513..515(+)
Misc Feature(3)1035..1037(+)
Misc Feature(4)1305..1580(+)
Misc Feature(5)1305..1424(+)
Misc Feature(6)1593..2078(+)
Misc Feature(7)1749..1751(+)
Misc Feature(8)1974..1976(+)
Misc Feature(9)2037..2039(+)
Misc Feature(10)2127..2129(+)
Misc Feature(11)2127..2129(+)
Misc Feature(12)2127..2129(+)
Misc Feature(13)2139..2141(+)
Misc Feature(14)2139..2141(+)
Misc Feature(15)2139..2141(+)
Misc Feature(16)2169..2171(+)
Misc Feature(17)2169..2171(+)
Misc Feature(18)2175..2177(+)
Misc Feature(19)2175..2177(+)
Misc Feature(20)2178..2180(+)
Misc Feature(21)2178..2180(+)
Misc Feature(22)2184..2186(+)
Misc Feature(23)2184..2186(+)
Misc Feature(24)2184..2186(+)
Misc Feature(25)2232..2240(+)
Misc Feature(26)2232..2234(+)
Exon (1)1..1110
Gene:PTEN
Gene Synonym:
Exon (2)1111..1195
Gene:PTEN
Gene Synonym:
Exon (3)1196..1240
Gene:PTEN
Gene Synonym:
Exon (4)1241..1284
Gene:PTEN
Gene Synonym:
Exon (5)1285..1523
Gene:PTEN
Gene Synonym:
Exon (6)1524..1665
Gene:PTEN
Gene Synonym:
Exon (7)1666..1832
Gene:PTEN
Gene Synonym:
Exon (8)1833..2057
Gene:PTEN
Gene Synonym:
Exon (9)2058..8701
Gene:PTEN
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000314
Organism Homo sapiens (human)
Definition Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000314

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGACAGCCA TCATCAAAGA GATCGTTAGC AGAAACAAAA GGAGATATCA AGAGGATGGA 
TTCGACTTAG ACTTGACCTA TATTTATCCA AACATTATTG CTATGGGATT TCCTGCAGAA
AGACTTGAAG GCGTATACAG GAACAATATT GATGATGTAG TAAGGTTTTT GGATTCAAAG
CATAAAAACC ATTACAAGAT ATACAATCTT TGTGCTGAAA GACATTATGA CACCGCCAAA
TTTAATTGCA GAGTTGCACA ATATCCTTTT GAAGACCATA ACCCACCACA GCTAGAACTT
ATCAAACCCT TTTGTGAAGA TCTTGACCAA TGGCTAAGTG AAGATGACAA TCATGTTGCA
GCAATTCACT GTAAAGCTGG AAAGGGACGA ACTGGTGTAA TGATATGTGC ATATTTATTA
CATCGGGGCA AATTTTTAAA GGCACAAGAG GCCCTAGATT TCTATGGGGA AGTAAGGACC
AGAGACAAAA AGGGAGTAAC TATTCCCAGT CAGAGGCGCT ATGTGTATTA TTATAGCTAC
CTGTTAAAGA ATCATCTGGA TTATAGACCA GTGGCACTGT TGTTTCACAA GATGATGTTT
GAAACTATTC CAATGTTCAG TGGCGGAACT TGCAATCCTC AGTTTGTGGT CTGCCAGCTA
AAGGTGAAGA TATATTCCTC CAATTCAGGA CCCACACGAC GGGAAGACAA GTTCATGTAC
TTTGAGTTCC CTCAGCCGTT ACCTGTGTGT GGTGATATCA AAGTAGAGTT CTTCCACAAA
CAGAACAAGA TGCTAAAAAA GGACAAAATG TTTCACTTTT GGGTAAATAC ATTCTTCATA
CCAGGACCAG AGGAAACCTC AGAAAAAGTA GAAAATGGAA GTCTATGTGA TCAAGAAATC
GATAGCATTT GCAGTATAGA GCGTGCAGAT AATGACAAGG AATATCTAGT ACTTACTTTA
ACAAAAAATG ATCTTGACAA AGCAAATAAA GACAAAGCCA ACCGATACTT TTCTCCAAAT
TTTAAGGTGA AGCTGTACTT CACAAAAACA GTAGAGGAGC CGTCAAATCC AGAGGCTAGC
AGTTCAACTT CTGTAACACC AGATGTTAGT GACAATGAAC CTGATCATTA TAGATATTCT
GACACCACTG ACTCTGATCC AGAGAATGAA CCTTTTGATG AAGATCAGCA TACACAAATT
ACAAAAGTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Heterogeneity of PTEN and ERG expression in prostate cancer on core needle biopsies: implications for cancer risk stratification and biomarker sampling
Hum. Pathol. 46 (5), 698-706 (2015)
Shah RB, Bentley J, Jeffery Z and DeMarzo AM.


book

miR-200a, miR-200b and miR-429 are onco-miRs that target the PTEN gene in endometrioid endometrial carcinoma
Anticancer Res. 35 (3), 1401-1410 (2015)
Yoneyama K, Ishibashi O, Kawase R, Kurose K and Takeshita T.


book

Conformational stability and catalytic activity of PTEN variants linked to cancers and autism spectrum disorders
Biochemistry 54 (7), 1576-1582 (2015)
Johnston SB and Raines RT.


book

Phosphorylation of PTEN at STT motif is associated with DNA damage response
Mutat. Res. 770, 112-119 (2014)
Misra S, Mukherjee A and Karmakar P.


book

PTENalpha, a PTEN isoform translated through alternative initiation, regulates mitochondrial function and energy metabolism
Cell Metab. 19 (5), 836-848 (2014)
Liang H, He S, Yang J, Jia X, Wang P, Chen X, Zhang Z, Zou X, McNutt MA, Shen WH and Yin Y.


book

A secreted PTEN phosphatase that enters cells to alter signaling and survival
Science 341 (6144), 399-402 (2013)
Hopkins BD, Fine B, Steinbach N, Dendy M, Rapp Z, Shaw J, Pappas K, Yu JS, Hodakoski C, Mense S, Klein J, Pegno S, Sulis ML, Goldstein H, Amendolara B, Lei L, Maurer M, Bruce J, Canoll P, Hibshoosh H and Parsons R.


book

Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers
Nat. Genet. 15 (4), 356-362 (1997)
Steck PA, Pershouse MA, Jasser SA, Yung WK, Lin H, Ligon AH, Langford LA, Baumgard ML, Hattier T, Davis T, Frye C, Hu R, Swedlund B, Teng DH and Tavtigian SV.


book

PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer
Science 275 (5308), 1943-1947 (1997)
Li J, Yen C, Liaw D, Podsypanina K, Bose S, Wang SI, Puc J, Miliaresis C, Rodgers L, McCombie R, Bigner SH, Giovanella BC, Ittmann M, Tycko B, Hibshoosh H, Wigler MH and Parsons R.


book

Allelic loss of sequences from the long arm of chromosome 10 and replication errors in endometrial cancers
Cancer Res. 55 (9), 1922-1926 (1995)
Peiffer SL, Herzog TJ, Tribune DJ, Mutch DG, Gersell DJ and Goodfellow PJ.


book

PTEN Hamartoma Tumor Syndrome (PHTS)
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Eng,C.


 
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