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PTGS2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol PTGS2
Entrez Gene ID 5743
Full Name prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Synonyms COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2
General protein information
Preferred Names
prostaglandin G/H synthase 2
Names
prostaglandin G/H synthase 2
PHS II
PGH synthase 2
cyclooxygenase 2b
prostaglandin H2 synthase 2
NP_000954.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q25.2-q25.3

Summary Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]. lac of sum
Disorder MIM:

600262


mRNA and Protein(s)

mRNA Protein Name
NM_000963 NP_000954 prostaglandin G/H synthase 2 precursor



Homo sapiens (human) PTGS2 NP_000954.1
Pan troglodytes (chimpanzee) PTGS2 XP_524999.3
Macaca mulatta (Rhesus monkey) PTGS2 XP_001107538.2
Canis lupus familiaris (dog) PTGS2 NP_001003354.1
Bos taurus (cattle) PTGS2 NP_776870.1
Mus musculus (house mouse) Ptgs2 NP_035328.2
Rattus norvegicus (Norway rat) Ptgs2 NP_058928.3
Gallus gallus (chicken) PTGS2 NP_001161191.1
Danio rerio (zebrafish) ptgs2b NP_001020675.1
Xenopus (Silurana) tropicalis (western clawed frog) ptgs2 NP_001025697.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PTGS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTGS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu27989
NM_000963 Homo sapiens prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
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$379.00
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu27989
Clone ID Related Accession (Same CDS sequence) NM_000963
Accession Version NM_000963.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1815bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product prostaglandin G/H synthase 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA277667.1, BC013734.1, AL033533.5, KF455116.1 and BM978796.1. This sequence is a reference standard in the RefSeqGene project. On Jan 17, 2014 this sequence version replaced gi:223941909. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC013734.1, M90100.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGCTCGCCC GCGCCCTGCT GCTGTGCGCG GTCCTGGCGC TCAGCCATAC AGCAAATCCT 
TGCTGTTCCC ACCCATGTCA AAACCGAGGT GTATGTATGA GTGTGGGATT TGACCAGTAT
AAGTGCGATT GTACCCGGAC AGGATTCTAT GGAGAAAACT GCTCAACACC GGAATTTTTG
ACAAGAATAA AATTATTTCT GAAACCCACT CCAAACACAG TGCACTACAT ACTTACCCAC
TTCAAGGGAT TTTGGAACGT TGTGAATAAC ATTCCCTTCC TTCGAAATGC AATTATGAGT
TATGTGTTGA CATCCAGATC ACATTTGATT GACAGTCCAC CAACTTACAA TGCTGACTAT
GGCTACAAAA GCTGGGAAGC CTTCTCTAAC CTCTCCTATT ATACTAGAGC CCTTCCTCCT
GTGCCTGATG ATTGCCCGAC TCCCTTGGGT GTCAAAGGTA AAAAGCAGCT TCCTGATTCA
AATGAGATTG TGGAAAAATT GCTTCTAAGA AGAAAGTTCA TCCCTGATCC CCAGGGCTCA
AACATGATGT TTGCATTCTT TGCCCAGCAC TTCACGCATC AGTTTTTCAA GACAGATCAT
AAGCGAGGGC CAGCTTTCAC CAACGGGCTG GGCCATGGGG TGGACTTAAA TCATATTTAC
GGTGAAACTC TGGCTAGACA GCGTAAACTG CGCCTTTTCA AGGATGGAAA AATGAAATAT
CAGATAATTG ATGGAGAGAT GTATCCTCCC ACAGTCAAAG ATACTCAGGC AGAGATGATC
TACCCTCCTC AAGTCCCTGA GCATCTACGG TTTGCTGTGG GGCAGGAGGT CTTTGGTCTG
GTGCCTGGTC TGATGATGTA TGCCACAATC TGGCTGCGGG AACACAACAG AGTATGCGAT
GTGCTTAAAC AGGAGCATCC TGAATGGGGT GATGAGCAGT TGTTCCAGAC AAGCAGGCTA
ATACTGATAG GAGAGACTAT TAAGATTGTG ATTGAAGATT ATGTGCAACA CTTGAGTGGC
TATCACTTCA AACTGAAATT TGACCCAGAA CTACTTTTCA ACAAACAATT CCAGTACCAA
AATCGTATTG CTGCTGAATT TAACACCCTC TATCACTGGC ATCCCCTTCT GCCTGACACC
TTTCAAATTC ATGACCAGAA ATACAACTAT CAACAGTTTA TCTACAACAA CTCTATATTG
CTGGAACATG GAATTACCCA GTTTGTTGAA TCATTCACCA GGCAAATTGC TGGCAGGGTT
GCTGGTGGTA GGAATGTTCC ACCCGCAGTA CAGAAAGTAT CACAGGCTTC CATTGACCAG
AGCAGGCAGA TGAAATACCA GTCTTTTAAT GAGTACCGCA AACGCTTTAT GCTGAAGCCC
TATGAATCAT TTGAAGAACT TACAGGAGAA AAGGAAATGT CTGCAGAGTT GGAAGCACTC
TATGGTGACA TCGATGCTGT GGAGCTGTAT CCTGCCCTTC TGGTAGAAAA GCCTCGGCCA
GATGCCATCT TTGGTGAAAC CATGGTAGAA GTTGGAGCAC CATTCTCCTT GAAAGGACTT
ATGGGTAATG TTATATGTTC TCCTGCCTAC TGGAAGCCAA GCACTTTTGG TGGAGAAGTG
GGTTTTCAAA TCATCAACAC TGCCTCAATT CAGTCTCTCA TCTGCAATAA CGTGAAGGGC
TGTCCCTTTA CTTCATTCAG TGTTCCAGAT CCAGAGCTCA TTAAAACAGT CACCATCAAT
GCAAGTTCTT CCCGCTCCGG ACTAGATGAT ATCAATCCCA CAGTACTACT AAAAGAACGT
TCGACTGAAC TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000954.1
CDS138..1952
Misc Feature(1)192..299(+)
Misc Feature(2)195..239(+)
Misc Feature(3)360..1823(+)
Misc Feature(4)444..1697(+)
Misc Feature(5)468..1751(+)
Misc Feature(6)501..1817(+)
Misc Feature(7)537..1445(+)
Misc Feature(8)1683..1685(+)
Exon (1)1..189
Gene:PTGS2
Gene Synonym:
Exon (2)190..306
Gene:PTGS2
Gene Synonym:
Exon (3)307..450
Gene:PTGS2
Gene Synonym:
Exon (4)451..594
Gene:PTGS2
Gene Synonym:
Exon (5)595..776
Gene:PTGS2
Gene Synonym:
Exon (6)777..860
Gene:PTGS2
Gene Synonym:
Exon (7)861..1107
Gene:PTGS2
Gene Synonym:
Exon (8)1108..1394
Gene:PTGS2
Gene Synonym:
Exon (9)1395..1542
Gene:PTGS2
Gene Synonym:
Exon (10)1543..4493
Gene:PTGS2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000963
Organism Homo sapiens (human)
Definition Homo sapiens prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000963

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGCTCGCCC GCGCCCTGCT GCTGTGCGCG GTCCTGGCGC TCAGCCATAC AGCAAATCCT 
TGCTGTTCCC ACCCATGTCA AAACCGAGGT GTATGTATGA GTGTGGGATT TGACCAGTAT
AAGTGCGATT GTACCCGGAC AGGATTCTAT GGAGAAAACT GCTCAACACC GGAATTTTTG
ACAAGAATAA AATTATTTCT GAAACCCACT CCAAACACAG TGCACTACAT ACTTACCCAC
TTCAAGGGAT TTTGGAACGT TGTGAATAAC ATTCCCTTCC TTCGAAATGC AATTATGAGT
TATGTGTTGA CATCCAGATC ACATTTGATT GACAGTCCAC CAACTTACAA TGCTGACTAT
GGCTACAAAA GCTGGGAAGC CTTCTCTAAC CTCTCCTATT ATACTAGAGC CCTTCCTCCT
GTGCCTGATG ATTGCCCGAC TCCCTTGGGT GTCAAAGGTA AAAAGCAGCT TCCTGATTCA
AATGAGATTG TGGAAAAATT GCTTCTAAGA AGAAAGTTCA TCCCTGATCC CCAGGGCTCA
AACATGATGT TTGCATTCTT TGCCCAGCAC TTCACGCATC AGTTTTTCAA GACAGATCAT
AAGCGAGGGC CAGCTTTCAC CAACGGGCTG GGCCATGGGG TGGACTTAAA TCATATTTAC
GGTGAAACTC TGGCTAGACA GCGTAAACTG CGCCTTTTCA AGGATGGAAA AATGAAATAT
CAGATAATTG ATGGAGAGAT GTATCCTCCC ACAGTCAAAG ATACTCAGGC AGAGATGATC
TACCCTCCTC AAGTCCCTGA GCATCTACGG TTTGCTGTGG GGCAGGAGGT CTTTGGTCTG
GTGCCTGGTC TGATGATGTA TGCCACAATC TGGCTGCGGG AACACAACAG AGTATGCGAT
GTGCTTAAAC AGGAGCATCC TGAATGGGGT GATGAGCAGT TGTTCCAGAC AAGCAGGCTA
ATACTGATAG GAGAGACTAT TAAGATTGTG ATTGAAGATT ATGTGCAACA CTTGAGTGGC
TATCACTTCA AACTGAAATT TGACCCAGAA CTACTTTTCA ACAAACAATT CCAGTACCAA
AATCGTATTG CTGCTGAATT TAACACCCTC TATCACTGGC ATCCCCTTCT GCCTGACACC
TTTCAAATTC ATGACCAGAA ATACAACTAT CAACAGTTTA TCTACAACAA CTCTATATTG
CTGGAACATG GAATTACCCA GTTTGTTGAA TCATTCACCA GGCAAATTGC TGGCAGGGTT
GCTGGTGGTA GGAATGTTCC ACCCGCAGTA CAGAAAGTAT CACAGGCTTC CATTGACCAG
AGCAGGCAGA TGAAATACCA GTCTTTTAAT GAGTACCGCA AACGCTTTAT GCTGAAGCCC
TATGAATCAT TTGAAGAACT TACAGGAGAA AAGGAAATGT CTGCAGAGTT GGAAGCACTC
TATGGTGACA TCGATGCTGT GGAGCTGTAT CCTGCCCTTC TGGTAGAAAA GCCTCGGCCA
GATGCCATCT TTGGTGAAAC CATGGTAGAA GTTGGAGCAC CATTCTCCTT GAAAGGACTT
ATGGGTAATG TTATATGTTC TCCTGCCTAC TGGAAGCCAA GCACTTTTGG TGGAGAAGTG
GGTTTTCAAA TCATCAACAC TGCCTCAATT CAGTCTCTCA TCTGCAATAA CGTGAAGGGC
TGTCCCTTTA CTTCATTCAG TGTTCCAGAT CCAGAGCTCA TTAAAACAGT CACCATCAAT
GCAAGTTCTT CCCGCTCCGG ACTAGATGAT ATCAATCCCA CAGTACTACT AAAAGAACGT
TCGACTGAAC TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A prospective study of macrophage inhibitory cytokine-1 (MIC-1/GDF15) and risk of colorectal cancer
J. Natl. Cancer Inst. 106 (4), DJU016 (2014)
Mehta RS, Song M, Bezawada N, Wu K, Garcia-Albeniz X, Morikawa T, Fuchs CS, Ogino S, Giovannucci EL and Chan AT.


book

NF-kappaB and COX-2 expression in nonmalignant endometrial lesions and cancer
Am. J. Clin. Pathol. 141 (2), 196-203 (2014)
Faloppa CC, Baiocchi G, Cunha IW, Fregnani JH, Osorio CA, Fukazawa EM, Kumagai LY, Badiglian-Filho L, Pinto GL and Soares FA.


book

Altered expression of inflammation-associated genes in oviductal cells following follicular fluid exposure: implications for ovarian carcinogenesis
Exp. Biol. Med. (Maywood) 239 (1), 24-32 (2014)
Lau A, Kollara A, St John E, Tone AA, Virtanen C, Greenblatt EM, King WA and Brown TJ.


book

Expression and significance of cyclooxygenase-2 mRNA in benign and malignant ascites
World J. Gastroenterol. 19 (40), 6883-6887 (2013)
Lu J, Li XF, Kong LX, Ma L, Liao SH and Jiang CY.


book

Lentivirus-mediated shRNA interference targeting cyclooxygenase-2 inhibits growth of human non-small cell lung cancer
J BUON 18 (4), 908-914 (2013)
Li T, Lu J and Zhong Y.


book

Structure of the human cyclo-oxygenase-2 gene
Biochem. J. 302 (PT 3), 723-727 (1994)
Appleby SB, Ristimaki A, Neilson K, Narko K and Hla T.


book

Inducible isoforms of cyclooxygenase and nitric-oxide synthase in inflammation
Proc. Natl. Acad. Sci. U.S.A. 91 (6), 2046-2050 (1994)
Vane JR, Mitchell JA, Appleton I, Tomlinson A, Bishop-Bailey D, Croxtall J and Willoughby DA.


book

Human cyclooxygenase-2 cDNA
Proc. Natl. Acad. Sci. U.S.A. 89 (16), 7384-7388 (1992)
Hla T and Neilson K.


book

Prostaglandin endoperoxide synthase gene structure: identification of the transcriptional start site and 5'-flanking regulatory sequences
Arch. Biochem. Biophys. 293 (2), 391-400 (1992)
Kraemer SA, Meade EA and DeWitt DL.


book

Detection and isolation of an endoperoxide intermediate in prostaglandin biosynthesis
Proc. Natl. Acad. Sci. U.S.A. 70 (3), 899-903 (1973)
Hamberg,M. and Samuelsson,B.


 
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