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SPTBN4 cDNA ORF clone, Homo sapiens (human)

Gene Symbol SPTBN4
Entrez Gene ID 57731
Full Name spectrin, beta, non-erythrocytic 4
Synonyms QV, SPNB4, SPTBN3
General protein information
Preferred Names
spectrin beta chain, non-erythrocytic 4
Names
spectrin beta chain, non-erythrocytic 4
beta-IV spectrin
spectrin beta chain, brain 3
spectrin, non-erythroid beta chain 3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19q13.13

Summary Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. This gene is one member of a family of beta-spectrin genes. The encoded protein localizes to the nuclear matrix, PML nuclear bodies, and cytoplasmic vesicles. A highly similar gene in the mouse is required for localization of specific membrane proteins in polarized regions of neurons. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

606214


mRNA and Protein(s)

mRNA Protein Name
XM_011527172 XP_011525474 spectrin beta chain, non-erythrocytic 4 isoform X1
XM_011527173 XP_011525475 spectrin beta chain, non-erythrocytic 4 isoform X2
NM_020971 NP_066022 spectrin beta chain, non-erythrocytic 4 isoform sigma1
NM_025213 NP_079489 spectrin beta chain, non-erythrocytic 4 isoform sigma5



Homo sapiens (human) SPTBN4 NP_066022.2
Macaca mulatta (Rhesus monkey) SPTBN4 XP_002801295.1
Canis lupus familiaris (dog) SPTBN4 XP_541613.2
Bos taurus (cattle) SPTBN4 NP_001179839.1
Mus musculus (house mouse) Sptbn4 NP_115999.2
Rattus norvegicus (Norway rat) Sptbn4 NP_001094315.1
Xenopus (Silurana) tropicalis (western clawed frog) sptbn4 XP_002938861.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPTBN4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTBN4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19524 XM_011527172 PREDICTED: Homo sapiens spectrin, beta, non-erythrocytic 4 (SPTBN4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1301.30
$1859.00
OHu70939 XM_011527173 PREDICTED: Homo sapiens spectrin, beta, non-erythrocytic 4 (SPTBN4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1301.30
$1859.00
OHu19524 NM_020971 Homo sapiens spectrin, beta, non-erythrocytic 4 (SPTBN4), transcript variant sigma1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1301.30
$1859.00
OHu04416 NM_025213 Homo sapiens spectrin, beta, non-erythrocytic 4 (SPTBN4), transcript variant sigma5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $482.30
$689.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19524
Clone ID Related Accession (Same CDS sequence) NM_020971 , XM_011527172
Accession Version XM_011527172.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 7695bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product spectrin beta chain, non-erythrocytic 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011109.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGCC TGCCTGCTCC TAACAACAAC 
CCTGCTGCCC GCTGGGAGAG TCCGGATCGG GGCTGGGAGC GGGAGCAGCC GGCTGCGTCC
ACCGCAGCGG CCTCGCTCTT TGAGTGCTCC CGGATCAAGG CCTTGGCAGA TGAGCGGGAA
GCCGTGCAGA AGAAAACCTT CACCAAGTGG GTGAACTCGC ACCTCGCCCG CGTGGGCTGC
CACATCGGGG ACCTCTATGT GGACCTCCGG GACGGCTTCG TGCTCACGCG GCTCCTGGAA
GTGCTGTCTG GGGAGCAGCT GCCCAGGCCC ACGCGCGGCC GCATGCGGAT CCACTCACTG
GAGAACGTGG ACAAGGCGCT GCAGTTTCTG AAGGAGCAGC GCGTGCACCT GGAGAACGTG
GGTTCGCATG ACATCGTGGA TGGGAATCAC CGGCTGACGC TGGGGCTGGT CTGGACCATC
ATCCTGCGCT TCCAGATTCA AGTCATCAAA ATTGAGACTG AGGACAACAG AGAGACACGC
TCAGCCAAGG ATGCTCTGCT CTTGTGGTGT CAGATGAAGA CAGCTGGTTA CCCTGAGGTA
AACATCCAGA ATTTCACCAC CAGCTGGCGG GATGGCTTGG CCTTCAATGC CCTCATTCAC
CGGCACAGGC CTGATCTCGT GGACTTCAGC AAACTCACCA AGTCCAATGC CAACTACAAC
CTGCAGAGAG CCTTCCGCAC AGCTGAGCAG CACCTGGGGC TGGCGCGGCT GCTGGATCCT
GAAGATGTGA ACATGGAGGC TCCAGATGAG AAGTCCATCA TCACCTACGT GGTCTCTTTC
TACCACTATT TCTCCAAGAT GAAGGCTCTG GCTGTGGAGG GGAAGCGTAT CGGGAAGGTC
TTGGACCAGG TATTGGAGGT GGGGAAGATC ATAGAACGCT ACGAGGAGCT GGCGGCTGAG
CTGCTGGCCT GGATCCACCG CACCGTGGGC CTCATCAGCA ATCAGAAATT TGCCAACTCC
TTAAGTGGGG TGCAGCAGCA ACTCCAGGCT TTCACGGCCT ATTGCACGCT GGAGAAGCCT
GTCAAGTTCC AGGAGAAGGG GAACCTAGAG GTGCTGCTCT TCAGCATCCA GAGCAAACTG
CGTGCCTGCA ACCGTCGCCT CTTTGTGCCT CGGGAGGGCT GTGGCATCTG GGATATTGAC
AAGGCATGGG GTGAGCTGGA GAAGGCTGAG CATGAGCGGG AGGCTGCCCT ACGGGCTGAG
CTGATTCGGC AGGAGAAGCT GGAACTACTG GCACAGAGGT TTGACCACAA GGTGGCTATG
AGGGAGAGCT GGCTGAATGA GAACCAGCGT CTGGTCTCCC AGGACAACTT TGGGTATGAG
CTGCCCGCAG TGGAGGCAGC CATGAAGAAA CACGAAGCGA TCGAGGCAGA CATTGCGGCC
TACGAGGAGC GGGTGCAGGG TGTGGCGGAG CTGGCCCAGG CATTGGCAGC CGAAGGCTAC
TACGATATCC GGCGGGTGGC AGCCCAGCGT GACAGCGTCC TGCGCCAGTG GGCCCTGCTA
ACTGGGCTTG TGGGTGCCCG GCGGACACGA CTTGAGCAGA ACCTTGCCCT GCAGAAGGTC
TTCCAGGAGA TGGTGTACAT GGTGGACTGG ATGGAGGAGA TGCAGGCTCA GCTGCTGTCC
CGGGAGTGTG GGCAGCACCT GGTGGAGGCA GACGACCTGT TGCAGAAGCA TGGACTGCTG
GAGGGAGACA TTGCCGCCCA GAGCGAGCGG GTGGAGGCTC TCAATGCCGC TGCCCTGCGC
TTCTCCCAGC TGCAGGGCTA CCAGCCCTGC GACCCGCAGG TCATCTGCAA CCGCGTGAAC
CACGTGCACG GCTGCCTGGC GGAGCTGCAG GAGCAGGCAG CGCGGCGACG CGCGGAGCTG
GAGGCTTCGC GGAGCCTGTG GGCGCTGCTG CAGGAGCTGG AGGAGGCCGA GAGCTGGGCG
CGCGACAAGG AGCGTCTCCT GGAGGCTGCG GGCGGCGGCG GTGCGGCGGG CGCAGCGGGC
GCAGCGGGAA CAGCGGGCGG CGCGCATGAC CTGTCCAGCA CAGCGCGCCT CCTGGCCCAG
CACAAGATCC TGCAGGGCGA GCTGGGCGGG CGGCGAGCGT TGCTGCAGCA GGCCCTGCGG
TGTGGCGAGG AGCTGGTTGC GGCCGGCGGT GCCGTCGGCC CGGGAGCAGA CACCGTGCAC
CTGGTAGGCC TGGCGGAGCG CGCGGCGAGC GCCCGGCGCC GCTGGCAGAG GCTGGAAGAG
GCGGCGGCGC GGCGAGAGCG GCGGCTGCAG GAGGCGCGGG CGCTGCACCA GTTCGGCGCT
GACCTCGACG GGCTGCTGGA CTGGCTTCGC GACGCTTACC GCCTGGCAGC CGCCGGTGAC
TTCGGCCACG ACGAAGCTTC CAGCCGCCGC CTGGCGCGCC AGCACCGCGC GCTCACCGGG
GAGGTGGAGG CACATCGCGG GCCCGTGAGC GGCCTGCGGC GCCAGCTGGC GACACTCGGG
GGTGCCAGTG GCGCAGGGCC ACTGGTGGTG GCGCTGCAGG TGCGCGTGGT GGAAGCAGAG
CAGTTGTTCG CTGAGGTGAC CGAAGTGGCG GCGCTGAGGC GCCAGTGGCT GCGGGACGCG
CTCGCTGTCT ACCGCATGTT TGGCGAGGTG CACGCGTGTG AGCTGTGGAT CGGCGAGAAG
GAGCAATGGC TGCTCTCCAT GCGTGTGCCG GATTCACTCG ACGACGTCGA GGTGGTGCAG
CACCGATTCG AGAGCCTGGA CCAAGAGATG AACAGCCTGA TGGGCCGCGT TCTGGACGTG
AACCACACAG TCCAGGAGCT GGTGGAAGGA GGCCACCCCA GTTCAGATGA GGTGCGTTCC
TGCCAGGACC ACCTCAACAG CAGGTGGAAC CGCATCGTGG AGCTAGTGGA ACAGCGCAAA
GAGGAAATGA GCGCGGTGCT GCTGGTGGAG AACCACGTGC TGGAGGTGGC CGAGGTGCGC
GCCCAGGTGC GTGAGAAGCG GAGAGCTGTG GAGAGCGCGC CCCGGGCCGG CGGCGCCCTG
CAGTGGCGTC TTAGCGGCCT AGAGGCCGCT CTGCAGGCGC TGGAGCCGCG CCAGGCGGCC
CTTCTGGAGG AGGCAGCCCT GCTGGCTGAG CGCTTCCCGG CGCAGGCGGC GCGGCTGCAC
CAGGGCGCGG AGGAGCTGGG CGCCGAGTGG GGCGCGCTAG CTAGCGCGGC TCAGGCCTGC
GGCGAGGCGG TGGCGGCAGC AGGGCGCCTG CAGCGCTTCC TACATGACCT CGACGCTTTC
CTGGACTGGC TCGTGCGCGC CCAGGAGGCG GCGGGCGGCA GCGAGGGGCC CCTGCCCAAC
AGCCTAGAAG AGGCGGACGC GCTGCTGGCG CGCCACGCTG CGCTCAAGGA GGAGGTGGAC
CAGCGCGAGG AAGACTATGC TCGCATCGTG GCGGCCAGCG AGGCGCTGCT GGCCGCCGAC
GGCGCAGAGC TGGGCCCGGG CCTGGCACTA GACGAGTGGC TGCCACACCT CGAACTTGGC
TGGCATAAAC TGCTCGGCTT GTGGGAGGCG CGCAGGGAGG CGCTGGTCCA GGCGCACATC
TACCAGCTCT TCCTGCGGGA TCTACGCCAG GCGCTCGTGG TGCTGCGTAA CCAGGAGATG
GCGCTGTCTG GTGCGGAGCT CCCGGGCACA GTGGAATCGG TGGAGGAGGC CTTGAAACAG
CACCGTGACT TTCTCACCAC CATGGAGCTG AGCCAGCAAA AGATGCAGGT GGCCGTGCAG
GCTGCAGAGG GCCTGCTGAG GCAGGGCAAC ATCTACGGGG AGCAGGCTCA GGAGGCTGTG
ACCCGGCTGC TGGAGAAGAA CCAAGAAAAC CAGTTACGGG CCCAGCAATG GATGCAAAAG
CTACATGACC AACTTGAGCT GCAGCACTTC CTCCGAGACT GCCACGAGCT GGATGGCTGG
ATCCATGAGA AGATGCTGAT GGCGCGGGAT GGCACGCGGG AGGACAACCA CAAGCTGCAT
AAGAGATGGC TCCGGCACCA GGCATTCATG GCCGAGCTGG CTCAGAATAA GGAGTGGCTG
GAGAAGATCG AGCGGGAGGG CCAGCAACTG ATGCAGGAGA AGCCCGAACT GGCGGCCTCC
GTGCGGAAGA AGCTGGGCGA GATCCGCCAG TGCTGGGCGG AGCTGGAGAG CACCACCCAG
GCCAAGGCAC GGCAGCTCTT TGAGGCCAGC AAAGCAGACC AGCTGGTGCA GAGCTTTGCT
GAGCTGGACA AGAAGCTCCT TCACATGGAG AGCCAGCTGC AAGACGTGGA CCCTGGAGGA
GACCTGGCCA CTGTCAACAG TCAGCTCAAG AAGCTGCAGT CCATGGAGTC GCAGGTGGAG
GAGTGGTACC GCGAGGTGGG AGAGCTGCAG GCGCAGACGG CGGCGCTGCC GCTGGAGCCG
GCGAGCAAGG AGCTGGTGGG TGAGCGGCAG AACGCGGTGG GCGAGCGCCT GGTGCGCCTG
CTCGAGCCGT TGCAGGAGCG CCGCCGCTTG CTGCTGGCTT CCAAGGAGTT GCACCAGGTG
GCGCACGACC TGGACGACGA GCTGGCATGG GTTCAGGAGC GGCTGCCACT GGCCATGCAG
ACAGAGCGAG GCAACGGTTT GCAGGCGGTC CAGCAGCACA TCAAAAAGAA CCAGGGCCTG
CGGCGGGAGA TCCAGGCGCA TGGGCCGCGC CTGGAGGAGG TGCTGGAGCG CGCGGGCGCG
CTGGCGTCGC TGCGCAGCCC GGAGGCAGAG GCAGTGCGCC GGGGCCTGGA GCAGCTGCAG
AGCGCCTGGG CCGGACTGCG GGAGGCTGCC GAGCGACGGC AGCAGGTGCT GGACGCCGCC
TTCCAGGTGG AGCAGTACTA CTTCGACGTG GCTGAGGTGG AGGCGTGGCT GGGCGAGCAG
GAGCTGCTCA TGATGAGTGA GGACAAGGGC AAGGACGAAC AGAGCACCCT GCAGCTGCTC
AAGAAACACC TGCAGCTGGA GCAAGGCGTG GAGAACTACG AGGAAAGCAT CGCGCAGCTG
TCGCGCCAGT GCCGGGCGCT GCTGGAGATG GGGCACCCGG ACAGCGAGCA GATCAGCCGG
CGGCAGTCTC AGGTGGACCG CCTGTACGTG GCGCTCAAGG AGCTGGGTGA GGAGCGCCGG
GTGGCTCTGG AACAGCAGTA CTGGCTGTAC CAGCTCAGCC GCCAGGTGAG CGAGCTTGAG
CACTGGATTG CCGAGAAGGA GGTGGTGGCT GGCTCACCCG AGCTCGGCCA GGACTTTGAG
CATGTCTCGG TGCTGCAGGA GAAATTCTCA GAGTTTGCCA GCGAGACAGG TATGGCAGGG
CGGGAACGGC TGGCAGCTGT GAACCAGATG GTGGATGAGC TGATCGAGTG TGGCCATACA
GCAGCGGCCA CCATGGCCGA GTGGAAGGAC GGACTGAACG AGGCCTGGGC TGAGCTGCTG
GAGCTCATGG GCACACGGGC CCAGCTGCTG GCCGCCTCTC GGGAGCTTCA TAAGTTCTTC
AGTGACGCCC GAGAGCTTCA GGGACAGATT GAGGAGAAGC GGAGGCGGCT GCCCCGCCTG
ACCACCCCGC CTGAGCCGAG ACCCAGTGCC AGTTCCATGC AGCGGACCCT GAGAGCCTTT
GAGCATGACC TGCAGCTCCT CGTGTCCCAG GTACGGCAGC TGCAGGAGGG GGCGGCCCAG
CTGCGGACGG TGTATGCGGG TGAACATGCC GAGGCCATCG CTAGCCGGGA GCAGGAGGTG
CTGCAGGGTT GGAAAGAGCT GCTGTCAGCC TGTGAGGATG CCCGCCTGCA TGTCAGCTCC
ACAGCCGACG CCCTGCGCTT CCACAGCCAA GTCCGCGACC TGCTCTCCTG GATGGATGGC
ATCGCCAGCC AGATTGGGGC AGCCGACAAG CCCAGGGACG TGTCATCAGT GGAGGTGCTC
ATGAACTACC ACCAGGGCCT GAAGACTGAG CTGGAGGCGC GGGTGCCTGA GCTGACCACC
TGCCAGGAGC TGGGGCGATC TCTGCTGCTC AACAAAAGTG CCATGGCTGA TGAGATCCAG
GCACAGCTGG ACAAGCTGGG AACCAGGAAG GAGGAGGTGT CGGAAAAGTG GGACCGCCAT
TGGGAGTGGC TGCAGCAGAT GCTGGAGGTG CACCAGTTTG CCCAGGAGGC GGTGGTGGCT
GATGCCTGGC TGACAGCCCA GGAGCCGCTC CTGCAGAGCC GGGAGCTGGG CAGCAGCGTG
GATGAGGTGG AGCAGCTTAT CCGGCGACAT GAGGCCTTCC GCAAAGCGGC TGCAGCCTGG
GAAGAGAGGT TCAGCTCTCT GCGGCGCCTG ACCACGATCG AGAAAATCAA AGCGGAACAG
AGCAAGCAGC CGCCTACCCC ACTGCTGGGG CGCAAGTTCT TTGGGGACCC CACGGAACTG
GCGGCCAAGG CGGCGCCCCT GCTGCGGCCA GGGGGCTATG AAAGGGGCTT GGAGCCCCTG
GCCCGCCGAG CCTCGGACAC GCTCTCGGCC GAGGTGCGGA CTCGGGTGGG GTATGTGCGC
CAGGAGCTCA AGCCCGAGCG CCTCCAGCCG CGCATTGACC GGCTGCCGGA GATCCCGGGG
AGGGTGGAGC CCGCGGCCCT GCCGGCCGCA CCAGAGGACG CGGCGGAGAC CCCCGCGACC
CCCGCGGCGG CGGAGCAGGT GCGGCCACGA CCGGAGCGCC AGGAGTCAGC TGATCGCGCG
GAGGAGCTGC CCAGGAGGCG GCGGCCTGAG CGGCAAGAGT CAGTCGATCA ATCCGAGGAG
GCTGCGCGGA GGCGGCGGCC GGAGCGGCAG GAGTCAGCGG AGCACGAGGC GGCACACAGC
CTTACCCTGG GCCGCTATGA GCAGATGGAG CGGCGGCGCG AGCGGCGTGA GCGGCGCTTG
GAGCGGCAGG AGTCCAGCGA ACAGGAGATG CCCATCAGAG GAGACCTGGT CAAGGGGAAG
GCCACCCTGG CTGACATTGT GGAACAGCTG CAGGAGAAAG AGGCAGGCCC AGGGCTGCCT
GCTGGGCCGT CGCTGCCTCA GCCACGCGAG CTTCCCCCAG GTCGCCTGCC CAACGGGCTT
GAGCTGCCCG AGCGGACACC TCGGCCGGAC CGGCCCCGGG CGCGGGACCG GCCCAAGCCG
CGACGGCGGC CGCGGCCCAG AGAGGGTGGT GAGGGCGGGG GAAGCCGGCG CTCGCGCTCC
GCCCCGGCCC AGGGCGGCTC CGCCCCCGCG CCTCCGCCAC CGCCCACTCA CACAGTGCAG
CACGAGGGCT TCCTACTGCG CAAGCGCGAG CTCGACGCTA ACCGCAAGTC GTCCAACCGG
TCGTGGGTGA GCCTGTACTG TGTGCTTAGT AAGGGGGAAC TGGGCTTCTA CAAGGACTCC
AAGGGCCCGG CATCCGGGAG CACACACGGT GGGGAACCGC TGCTCAGCCT GCACAAGGCC
ACCAGCGAGG TGGCTAGTGA CTACAAGAAA AAGAAGCATG TCTTCAAGCT CCAGACCCAG
GATGGCAGTG AGTTTTTGCT CCAGGCAAAA GATGAGGAGG AGATGAACGG CTGGCTGGAG
GCTGTAGCTT CCTCGGTGGC GGAACACGCA GAGATCGCCC GCTGGGGCCA GACACTACCC
ACTACTTCAT CCACAGATGA GGGCAACCCT AAGAGGGAAG GCGGAGATCG CAGGGCCAGC
GGGCGCAGGA AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011525474.1
CDS830..8524
Misc Feature(1)1013..1324(+)
Misc Feature(2)1013..1318(+)
Misc Feature(3)1370..1681(+)
Misc Feature(4)1370..1678(+)
Misc Feature(5)1760..2086(+)
Misc Feature(6)2117..2761(+)
Misc Feature(7)2429..2446(+)
Misc Feature(8)3152..3790(+)
Misc Feature(9)3467..3484(+)
Misc Feature(10)4100..4414(+)
Misc Feature(11)4430..5056(+)
Misc Feature(12)4739..4756(+)
Misc Feature(13)4745..5371(+)
Misc Feature(14)5054..5071(+)
Misc Feature(15)5375..6010(+)
Misc Feature(16)5687..5704(+)
Misc Feature(17)5693..6331(+)
Misc Feature(18)6005..6022(+)
Misc Feature(19)6329..6628(+)
Misc Feature(20)6656..>7159(+)
Misc Feature(21)6968..6985(+)
Misc Feature(22)8090..8404(+)
Misc Feature(23)8111..8293(+)
Translation

Target ORF information:

RefSeq Version XM_011527172
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens spectrin, beta, non-erythrocytic 4 (SPTBN4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011527172

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGCC TGCCTGCTCC TAACAACAAC 
CCTGCTGCCC GCTGGGAGAG TCCGGATCGG GGCTGGGAGC GGGAGCAGCC GGCTGCGTCC
ACCGCAGCGG CCTCGCTCTT TGAGTGCTCC CGGATCAAGG CCTTGGCAGA TGAGCGGGAA
GCCGTGCAGA AGAAAACCTT CACCAAGTGG GTGAACTCGC ACCTCGCCCG CGTGGGCTGC
CACATCGGGG ACCTCTATGT GGACCTCCGG GACGGCTTCG TGCTCACGCG GCTCCTGGAA
GTGCTGTCTG GGGAGCAGCT GCCCAGGCCC ACGCGCGGCC GCATGCGGAT CCACTCACTG
GAGAACGTGG ACAAGGCGCT GCAGTTTCTG AAGGAGCAGC GCGTGCACCT GGAGAACGTG
GGTTCGCATG ACATCGTGGA TGGGAATCAC CGGCTGACGC TGGGGCTGGT CTGGACCATC
ATCCTGCGCT TCCAGATTCA AGTCATCAAA ATTGAGACTG AGGACAACAG AGAGACACGC
TCAGCCAAGG ATGCTCTGCT CTTGTGGTGT CAGATGAAGA CAGCTGGTTA CCCTGAGGTA
AACATCCAGA ATTTCACCAC CAGCTGGCGG GATGGCTTGG CCTTCAATGC CCTCATTCAC
CGGCACAGGC CTGATCTCGT GGACTTCAGC AAACTCACCA AGTCCAATGC CAACTACAAC
CTGCAGAGAG CCTTCCGCAC AGCTGAGCAG CACCTGGGGC TGGCGCGGCT GCTGGATCCT
GAAGATGTGA ACATGGAGGC TCCAGATGAG AAGTCCATCA TCACCTACGT GGTCTCTTTC
TACCACTATT TCTCCAAGAT GAAGGCTCTG GCTGTGGAGG GGAAGCGTAT CGGGAAGGTC
TTGGACCAGG TATTGGAGGT GGGGAAGATC ATAGAACGCT ACGAGGAGCT GGCGGCTGAG
CTGCTGGCCT GGATCCACCG CACCGTGGGC CTCATCAGCA ATCAGAAATT TGCCAACTCC
TTAAGTGGGG TGCAGCAGCA ACTCCAGGCT TTCACGGCCT ATTGCACGCT GGAGAAGCCT
GTCAAGTTCC AGGAGAAGGG GAACCTAGAG GTGCTGCTCT TCAGCATCCA GAGCAAACTG
CGTGCCTGCA ACCGTCGCCT CTTTGTGCCT CGGGAGGGCT GTGGCATCTG GGATATTGAC
AAGGCATGGG GTGAGCTGGA GAAGGCTGAG CATGAGCGGG AGGCTGCCCT ACGGGCTGAG
CTGATTCGGC AGGAGAAGCT GGAACTACTG GCACAGAGGT TTGACCACAA GGTGGCTATG
AGGGAGAGCT GGCTGAATGA GAACCAGCGT CTGGTCTCCC AGGACAACTT TGGGTATGAG
CTGCCCGCAG TGGAGGCAGC CATGAAGAAA CACGAAGCGA TCGAGGCAGA CATTGCGGCC
TACGAGGAGC GGGTGCAGGG TGTGGCGGAG CTGGCCCAGG CATTGGCAGC CGAAGGCTAC
TACGATATCC GGCGGGTGGC AGCCCAGCGT GACAGCGTCC TGCGCCAGTG GGCCCTGCTA
ACTGGGCTTG TGGGTGCCCG GCGGACACGA CTTGAGCAGA ACCTTGCCCT GCAGAAGGTC
TTCCAGGAGA TGGTGTACAT GGTGGACTGG ATGGAGGAGA TGCAGGCTCA GCTGCTGTCC
CGGGAGTGTG GGCAGCACCT GGTGGAGGCA GACGACCTGT TGCAGAAGCA TGGACTGCTG
GAGGGAGACA TTGCCGCCCA GAGCGAGCGG GTGGAGGCTC TCAATGCCGC TGCCCTGCGC
TTCTCCCAGC TGCAGGGCTA CCAGCCCTGC GACCCGCAGG TCATCTGCAA CCGCGTGAAC
CACGTGCACG GCTGCCTGGC GGAGCTGCAG GAGCAGGCAG CGCGGCGACG CGCGGAGCTG
GAGGCTTCGC GGAGCCTGTG GGCGCTGCTG CAGGAGCTGG AGGAGGCCGA GAGCTGGGCG
CGCGACAAGG AGCGTCTCCT GGAGGCTGCG GGCGGCGGCG GTGCGGCGGG CGCAGCGGGC
GCAGCGGGAA CAGCGGGCGG CGCGCATGAC CTGTCCAGCA CAGCGCGCCT CCTGGCCCAG
CACAAGATCC TGCAGGGCGA GCTGGGCGGG CGGCGAGCGT TGCTGCAGCA GGCCCTGCGG
TGTGGCGAGG AGCTGGTTGC GGCCGGCGGT GCCGTCGGCC CGGGAGCAGA CACCGTGCAC
CTGGTAGGCC TGGCGGAGCG CGCGGCGAGC GCCCGGCGCC GCTGGCAGAG GCTGGAAGAG
GCGGCGGCGC GGCGAGAGCG GCGGCTGCAG GAGGCGCGGG CGCTGCACCA GTTCGGCGCT
GACCTCGACG GGCTGCTGGA CTGGCTTCGC GACGCTTACC GCCTGGCAGC CGCCGGTGAC
TTCGGCCACG ACGAAGCTTC CAGCCGCCGC CTGGCGCGCC AGCACCGCGC GCTCACCGGG
GAGGTGGAGG CACATCGCGG GCCCGTGAGC GGCCTGCGGC GCCAGCTGGC GACACTCGGG
GGTGCCAGTG GCGCAGGGCC ACTGGTGGTG GCGCTGCAGG TGCGCGTGGT GGAAGCAGAG
CAGTTGTTCG CTGAGGTGAC CGAAGTGGCG GCGCTGAGGC GCCAGTGGCT GCGGGACGCG
CTCGCTGTCT ACCGCATGTT TGGCGAGGTG CACGCGTGTG AGCTGTGGAT CGGCGAGAAG
GAGCAATGGC TGCTCTCCAT GCGTGTGCCG GATTCACTCG ACGACGTCGA GGTGGTGCAG
CACCGATTCG AGAGCCTGGA CCAAGAGATG AACAGCCTGA TGGGCCGCGT TCTGGACGTG
AACCACACAG TCCAGGAGCT GGTGGAAGGA GGCCACCCCA GTTCAGATGA GGTGCGTTCC
TGCCAGGACC ACCTCAACAG CAGGTGGAAC CGCATCGTGG AGCTAGTGGA ACAGCGCAAA
GAGGAAATGA GCGCGGTGCT GCTGGTGGAG AACCACGTGC TGGAGGTGGC CGAGGTGCGC
GCCCAGGTGC GTGAGAAGCG GAGAGCTGTG GAGAGCGCGC CCCGGGCCGG CGGCGCCCTG
CAGTGGCGTC TTAGCGGCCT AGAGGCCGCT CTGCAGGCGC TGGAGCCGCG CCAGGCGGCC
CTTCTGGAGG AGGCAGCCCT GCTGGCTGAG CGCTTCCCGG CGCAGGCGGC GCGGCTGCAC
CAGGGCGCGG AGGAGCTGGG CGCCGAGTGG GGCGCGCTAG CTAGCGCGGC TCAGGCCTGC
GGCGAGGCGG TGGCGGCAGC AGGGCGCCTG CAGCGCTTCC TACATGACCT CGACGCTTTC
CTGGACTGGC TCGTGCGCGC CCAGGAGGCG GCGGGCGGCA GCGAGGGGCC CCTGCCCAAC
AGCCTAGAAG AGGCGGACGC GCTGCTGGCG CGCCACGCTG CGCTCAAGGA GGAGGTGGAC
CAGCGCGAGG AAGACTATGC TCGCATCGTG GCGGCCAGCG AGGCGCTGCT GGCCGCCGAC
GGCGCAGAGC TGGGCCCGGG CCTGGCACTA GACGAGTGGC TGCCACACCT CGAACTTGGC
TGGCATAAAC TGCTCGGCTT GTGGGAGGCG CGCAGGGAGG CGCTGGTCCA GGCGCACATC
TACCAGCTCT TCCTGCGGGA TCTACGCCAG GCGCTCGTGG TGCTGCGTAA CCAGGAGATG
GCGCTGTCTG GTGCGGAGCT CCCGGGCACA GTGGAATCGG TGGAGGAGGC CTTGAAACAG
CACCGTGACT TTCTCACCAC CATGGAGCTG AGCCAGCAAA AGATGCAGGT GGCCGTGCAG
GCTGCAGAGG GCCTGCTGAG GCAGGGCAAC ATCTACGGGG AGCAGGCTCA GGAGGCTGTG
ACCCGGCTGC TGGAGAAGAA CCAAGAAAAC CAGTTACGGG CCCAGCAATG GATGCAAAAG
CTACATGACC AACTTGAGCT GCAGCACTTC CTCCGAGACT GCCACGAGCT GGATGGCTGG
ATCCATGAGA AGATGCTGAT GGCGCGGGAT GGCACGCGGG AGGACAACCA CAAGCTGCAT
AAGAGATGGC TCCGGCACCA GGCATTCATG GCCGAGCTGG CTCAGAATAA GGAGTGGCTG
GAGAAGATCG AGCGGGAGGG CCAGCAACTG ATGCAGGAGA AGCCCGAACT GGCGGCCTCC
GTGCGGAAGA AGCTGGGCGA GATCCGCCAG TGCTGGGCGG AGCTGGAGAG CACCACCCAG
GCCAAGGCAC GGCAGCTCTT TGAGGCCAGC AAAGCAGACC AGCTGGTGCA GAGCTTTGCT
GAGCTGGACA AGAAGCTCCT TCACATGGAG AGCCAGCTGC AAGACGTGGA CCCTGGAGGA
GACCTGGCCA CTGTCAACAG TCAGCTCAAG AAGCTGCAGT CCATGGAGTC GCAGGTGGAG
GAGTGGTACC GCGAGGTGGG AGAGCTGCAG GCGCAGACGG CGGCGCTGCC GCTGGAGCCG
GCGAGCAAGG AGCTGGTGGG TGAGCGGCAG AACGCGGTGG GCGAGCGCCT GGTGCGCCTG
CTCGAGCCGT TGCAGGAGCG CCGCCGCTTG CTGCTGGCTT CCAAGGAGTT GCACCAGGTG
GCGCACGACC TGGACGACGA GCTGGCATGG GTTCAGGAGC GGCTGCCACT GGCCATGCAG
ACAGAGCGAG GCAACGGTTT GCAGGCGGTC CAGCAGCACA TCAAAAAGAA CCAGGGCCTG
CGGCGGGAGA TCCAGGCGCA TGGGCCGCGC CTGGAGGAGG TGCTGGAGCG CGCGGGCGCG
CTGGCGTCGC TGCGCAGCCC GGAGGCAGAG GCAGTGCGCC GGGGCCTGGA GCAGCTGCAG
AGCGCCTGGG CCGGACTGCG GGAGGCTGCC GAGCGACGGC AGCAGGTGCT GGACGCCGCC
TTCCAGGTGG AGCAGTACTA CTTCGACGTG GCTGAGGTGG AGGCGTGGCT GGGCGAGCAG
GAGCTGCTCA TGATGAGTGA GGACAAGGGC AAGGACGAAC AGAGCACCCT GCAGCTGCTC
AAGAAACACC TGCAGCTGGA GCAAGGCGTG GAGAACTACG AGGAAAGCAT CGCGCAGCTG
TCGCGCCAGT GCCGGGCGCT GCTGGAGATG GGGCACCCGG ACAGCGAGCA GATCAGCCGG
CGGCAGTCTC AGGTGGACCG CCTGTACGTG GCGCTCAAGG AGCTGGGTGA GGAGCGCCGG
GTGGCTCTGG AACAGCAGTA CTGGCTGTAC CAGCTCAGCC GCCAGGTGAG CGAGCTTGAG
CACTGGATTG CCGAGAAGGA GGTGGTGGCT GGCTCACCCG AGCTCGGCCA GGACTTTGAG
CATGTCTCGG TGCTGCAGGA GAAATTCTCA GAGTTTGCCA GCGAGACAGG TATGGCAGGG
CGGGAACGGC TGGCAGCTGT GAACCAGATG GTGGATGAGC TGATCGAGTG TGGCCATACA
GCAGCGGCCA CCATGGCCGA GTGGAAGGAC GGACTGAACG AGGCCTGGGC TGAGCTGCTG
GAGCTCATGG GCACACGGGC CCAGCTGCTG GCCGCCTCTC GGGAGCTTCA TAAGTTCTTC
AGTGACGCCC GAGAGCTTCA GGGACAGATT GAGGAGAAGC GGAGGCGGCT GCCCCGCCTG
ACCACCCCGC CTGAGCCGAG ACCCAGTGCC AGTTCCATGC AGCGGACCCT GAGAGCCTTT
GAGCATGACC TGCAGCTCCT CGTGTCCCAG GTACGGCAGC TGCAGGAGGG GGCGGCCCAG
CTGCGGACGG TGTATGCGGG TGAACATGCC GAGGCCATCG CTAGCCGGGA GCAGGAGGTG
CTGCAGGGTT GGAAAGAGCT GCTGTCAGCC TGTGAGGATG CCCGCCTGCA TGTCAGCTCC
ACAGCCGACG CCCTGCGCTT CCACAGCCAA GTCCGCGACC TGCTCTCCTG GATGGATGGC
ATCGCCAGCC AGATTGGGGC AGCCGACAAG CCCAGGGACG TGTCATCAGT GGAGGTGCTC
ATGAACTACC ACCAGGGCCT GAAGACTGAG CTGGAGGCGC GGGTGCCTGA GCTGACCACC
TGCCAGGAGC TGGGGCGATC TCTGCTGCTC AACAAAAGTG CCATGGCTGA TGAGATCCAG
GCACAGCTGG ACAAGCTGGG AACCAGGAAG GAGGAGGTGT CGGAAAAGTG GGACCGCCAT
TGGGAGTGGC TGCAGCAGAT GCTGGAGGTG CACCAGTTTG CCCAGGAGGC GGTGGTGGCT
GATGCCTGGC TGACAGCCCA GGAGCCGCTC CTGCAGAGCC GGGAGCTGGG CAGCAGCGTG
GATGAGGTGG AGCAGCTTAT CCGGCGACAT GAGGCCTTCC GCAAAGCGGC TGCAGCCTGG
GAAGAGAGGT TCAGCTCTCT GCGGCGCCTG ACCACGATCG AGAAAATCAA AGCGGAACAG
AGCAAGCAGC CGCCTACCCC ACTGCTGGGG CGCAAGTTCT TTGGGGACCC CACGGAACTG
GCGGCCAAGG CGGCGCCCCT GCTGCGGCCA GGGGGCTATG AAAGGGGCTT GGAGCCCCTG
GCCCGCCGAG CCTCGGACAC GCTCTCGGCC GAGGTGCGGA CTCGGGTGGG GTATGTGCGC
CAGGAGCTCA AGCCCGAGCG CCTCCAGCCG CGCATTGACC GGCTGCCGGA GATCCCGGGG
AGGGTGGAGC CCGCGGCCCT GCCGGCCGCA CCAGAGGACG CGGCGGAGAC CCCCGCGACC
CCCGCGGCGG CGGAGCAGGT GCGGCCACGA CCGGAGCGCC AGGAGTCAGC TGATCGCGCG
GAGGAGCTGC CCAGGAGGCG GCGGCCTGAG CGGCAAGAGT CAGTCGATCA ATCCGAGGAG
GCTGCGCGGA GGCGGCGGCC GGAGCGGCAG GAGTCAGCGG AGCACGAGGC GGCACACAGC
CTTACCCTGG GCCGCTATGA GCAGATGGAG CGGCGGCGCG AGCGGCGTGA GCGGCGCTTG
GAGCGGCAGG AGTCCAGCGA ACAGGAGATG CCCATCAGAG GAGACCTGGT CAAGGGGAAG
GCCACCCTGG CTGACATTGT GGAACAGCTG CAGGAGAAAG AGGCAGGCCC AGGGCTGCCT
GCTGGGCCGT CGCTGCCTCA GCCACGCGAG CTTCCCCCAG GTCGCCTGCC CAACGGGCTT
GAGCTGCCCG AGCGGACACC TCGGCCGGAC CGGCCCCGGG CGCGGGACCG GCCCAAGCCG
CGACGGCGGC CGCGGCCCAG AGAGGGTGGT GAGGGCGGGG GAAGCCGGCG CTCGCGCTCC
GCCCCGGCCC AGGGCGGCTC CGCCCCCGCG CCTCCGCCAC CGCCCACTCA CACAGTGCAG
CACGAGGGCT TCCTACTGCG CAAGCGCGAG CTCGACGCTA ACCGCAAGTC GTCCAACCGG
TCGTGGGTGA GCCTGTACTG TGTGCTTAGT AAGGGGGAAC TGGGCTTCTA CAAGGACTCC
AAGGGCCCGG CATCCGGGAG CACACACGGT GGGGAACCGC TGCTCAGCCT GCACAAGGCC
ACCAGCGAGG TGGCTAGTGA CTACAAGAAA AAGAAGCATG TCTTCAAGCT CCAGACCCAG
GATGGCAGTG AGTTTTTGCT CCAGGCAAAA GATGAGGAGG AGATGAACGG CTGGCTGGAG
GCTGTAGCTT CCTCGGTGGC GGAACACGCA GAGATCGCCC GCTGGGGCCA GACACTACCC
ACTACTTCAT CCACAGATGA GGGCAACCCT AAGAGGGAAG GCGGAGATCG CAGGGCCAGC
GGGCGCAGGA AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu70939
Clone ID Related Accession (Same CDS sequence) XM_011527173
Accession Version XM_011527173.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 7056bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product spectrin beta chain, non-erythrocytic 4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011109.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGCC TGCCTGCTCC TAACAACAAC 
CCTGCTGCCC GCTGGGAGAG TCCGGATCGG GGCTGGGAGC GGGAGCAGCC GGCTGCGTCC
ACCGCAGCGG CCTCGCTCTT TGAGTGCTCC CGGATCAAGG CCTTGGCAGA TGAGCGGGAA
GCCGTGCAGA AGAAAACCTT CACCAAGTGG GTGAACTCGC ACCTCGCCCG CGTGGGCTGC
CACATCGGGG ACCTCTATGT GGACCTCCGG GACGGCTTCG TGCTCACGCG GCTCCTGGAA
GTGCTGTCTG GGGAGCAGCT GCCCAGGCCC ACGCGCGGCC GCATGCGGAT CCACTCACTG
GAGAACGTGG ACAAGGCGCT GCAGTTTCTG AAGGAGCAGC GCGTGCACCT GGAGAACGTG
GGTTCGCATG ACATCGTGGA TGGGAATCAC CGGCTGACGC TGGGGCTGGT CTGGACCATC
ATCCTGCGCT TCCAGATTCA AGTCATCAAA ATTGAGACTG AGGACAACAG AGAGACACGC
TCAGCCAAGG ATGCTCTGCT CTTGTGGTGT CAGATGAAGA CAGCTGGTTA CCCTGAGGTA
AACATCCAGA ATTTCACCAC CAGCTGGCGG GATGGCTTGG CCTTCAATGC CCTCATTCAC
CGGCACAGGC CTGATCTCGT GGACTTCAGC AAACTCACCA AGTCCAATGC CAACTACAAC
CTGCAGAGAG CCTTCCGCAC AGCTGAGCAG CACCTGGGGC TGGCGCGGCT GCTGGATCCT
GAAGATGTGA ACATGGAGGC TCCAGATGAG AAGTCCATCA TCACCTACGT GGTCTCTTTC
TACCACTATT TCTCCAAGAT GAAGGCTCTG GCTGTGGAGG GGAAGCGTAT CGGGAAGGTC
TTGGACCAGG TATTGGAGGT GGGGAAGATC ATAGAACGCT ACGAGGAGCT GGCGGCTGAG
CTGCTGGCCT GGATCCACCG CACCGTGGGC CTCATCAGCA ATCAGAAATT TGCCAACTCC
TTAAGTGGGG TGCAGCAGCA ACTCCAGGCT TTCACGGCCT ATTGCACGCT GGAGAAGCCT
GTCAAGTTCC AGGAGAAGGG GAACCTAGAG GTGCTGCTCT TCAGCATCCA GAGCAAACTG
CGTGCCTGCA ACCGTCGCCT CTTTGTGCCT CGGGAGGGCT GTGGCATCTG GGATATTGAC
AAGGCATGGG GTGAGCTGGA GAAGGCTGAG CATGAGCGGG AGGCTGCCCT ACGGGCTGAG
CTGATTCGGC AGGAGAAGCT GGAACTACTG GCACAGAGGT TTGACCACAA GGTGGCTATG
AGGGAGAGCT GGCTGAATGA GAACCAGCGT CTGGTCTCCC AGGACAACTT TGGGTATGAG
CTGCCCGCAG TGGAGGCAGC CATGAAGAAA CACGAAGCGA TCGAGGCAGA CATTGCGGCC
TACGAGGAGC GGGTGCAGGG TGTGGCGGAG CTGGCCCAGG CATTGGCAGC CGAAGGCTAC
TACGATATCC GGCGGGTGGC AGCCCAGCGT GACAGCGTCC TGCGCCAGTG GGCCCTGCTA
ACTGGGCTTG TGGGTGCCCG GCGGACACGA CTTGAGCAGA ACCTTGCCCT GCAGAAGGTC
TTCCAGGAGA TGGTGTACAT GGTGGACTGG ATGGAGGAGA TGCAGGCTCA GCTGCTGTCC
CGGGAGTGTG GGCAGCACCT GGTGGAGGCA GACGACCTGT TGCAGAAGCA TGGACTGCTG
GAGGGAGACA TTGCCGCCCA GAGCGAGCGG GTGGAGGCTC TCAATGCCGC TGCCCTGCGC
TTCTCCCAGC TGCAGGGCTA CCAGCCCTGC GACCCGCAGG TCATCTGCAA CCGCGTGAAC
CACGTGCACG GCTGCCTGGC GGAGCTGCAG GAGCAGGCAG CGCGGCGACG CGCGGAGCTG
GAGGCTTCGC GGAGCCTGTG GGCGCTGCTG CAGGAGCTGG AGGAGGCCGA GAGCTGGGCG
CGCGACAAGG AGCGTCTCCT GGAGGCTGCG GGCGGCGGCG GTGCGGCGGG CGCAGCGGGC
GCAGCGGGAA CAGCGGGCGG CGCGCATGAC CTGTCCAGCA CAGCGCGCCT CCTGGCCCAG
CACAAGATCC TGCAGGGCGA GCTGGGCGGG CGGCGAGCGT TGCTGCAGCA GGCCCTGCGG
TGTGGCGAGG AGCTGGTTGC GGCCGGCGGT GCCGTCGGCC CGGGAGCAGA CACCGTGCAC
CTGGTAGGCC TGGCGGAGCG CGCGGCGAGC GCCCGGCGCC GCTGGCAGAG GCTGGAAGAG
GCGGCGGCGC GGCGAGAGCG GCGGCTGCAG GAGGCGCGGG CGCTGCACCA GTTCGGCGCT
GACCTCGACG GGCTGCTGGA CTGGCTTCGC GACGCTTACC GCCTGGCAGC CGCCGGTGAC
TTCGGCCACG ACGAAGCTTC CAGCCGCCGC CTGGCGCGCC AGCACCGCGC GCTCACCGGG
GAGGTGGAGG CACATCGCGG GCCCGTGAGC GGCCTGCGGC GCCAGCTGGC GACACTCGGG
GGTGCCAGTG GCGCAGGGCC ACTGGTGGTG GCGCTGCAGG TGCGCGTGGT GGAAGCAGAG
CAGTTGTTCG CTGAGGTGAC CGAAGTGGCG GCGCTGAGGC GCCAGTGGCT GCGGGACGCG
CTCGCTGTCT ACCGCATGTT TGGCGAGGTG CACGCGTGTG AGCTGTGGAT CGGCGAGAAG
GAGCAATGGC TGCTCTCCAT GCGTGTGCCG GATTCACTCG ACGACGTCGA GGTGGTGCAG
CACCGATTCG AGAGCCTGGA CCAAGAGATG AACAGCCTGA TGGGCCGCGT TCTGGACGTG
AACCACACAG TCCAGGAGCT GGTGGAAGGA GGCCACCCCA GTTCAGATGA GGTGCGTTCC
TGCCAGGACC ACCTCAACAG CAGGTGGAAC CGCATCGTGG AGCTAGTGGA ACAGCGCAAA
GAGGAAATGA GCGCGGTGCT GCTGGTGGAG AACCACGTGC TGGAGGTGGC CGAGGTGCGC
GCCCAGGTGC GTGAGAAGCG GAGAGCTGTG GAGAGCGCGC CCCGGGCCGG CGGCGCCCTG
CAGTGGCGTC TTAGCGGCCT AGAGGCCGCT CTGCAGGCGC TGGAGCCGCG CCAGGCGGCC
CTTCTGGAGG AGGCAGCCCT GCTGGCTGAG CGCTTCCCGG CGCAGGCGGC GCGGCTGCAC
CAGGGCGCGG AGGAGCTGGG CGCCGAGTGG GGCGCGCTAG CTAGCGCGGC TCAGGCCTGC
GGCGAGGCGG TGGCGGCAGC AGGGCGCCTG CAGCGCTTCC TACATGACCT CGACGCTTTC
CTGGACTGGC TCGTGCGCGC CCAGGAGGCG GCGGGCGGCA GCGAGGGGCC CCTGCCCAAC
AGCCTAGAAG AGGCGGACGC GCTGCTGGCG CGCCACGCTG CGCTCAAGGA GGAGGTGGAC
CAGCGCGAGG AAGACTATGC TCGCATCGTG GCGGCCAGCG AGGCGCTGCT GGCCGCCGAC
GGCGCAGAGC TGGGCCCGGG CCTGGCACTA GACGAGTGGC TGCCACACCT CGAACTTGGC
TGGCATAAAC TGCTCGGCTT GTGGGAGGCG CGCAGGGAGG CGCTGGTCCA GGCGCACATC
TACCAGCTCT TCCTGCGGGA TCTACGCCAG GCGCTCGTGG TGCTGCGTAA CCAGGAGATG
GCGCTGTCTG GTGCGGAGCT CCCGGGCACA GTGGAATCGG TGGAGGAGGC CTTGAAACAG
CACCGTGACT TTCTCACCAC CATGGAGCTG AGCCAGCAAA AGATGCAGGT GGCCGTGCAG
GCTGCAGAGG GCCTGCTGAG GCAGGGCAAC ATCTACGGGG AGCAGGCTCA GGAGGCTGTG
ACCCGGCTGC TGGAGAAGAA CCAAGAAAAC CAGTTACGGG CCCAGCAATG GATGCAAAAG
CTACATGACC AACTTGAGCT GCAGCACTTC CTCCGAGACT GCCACGAGCT GGATGGCTGG
ATCCATGAGA AGATGCTGAT GGCGCGGGAT GGCACGCGGG AGGACAACCA CAAGCTGCAT
AAGAGATGGC TCCGGCACCA GGCATTCATG GCCGAGCTGG CTCAGAATAA GGAGTGGCTG
GAGAAGATCG AGCGGGAGGG CCAGCAACTG ATGCAGGAGA AGCCCGAACT GGCGGCCTCC
GTGCGGAAGA AGCTGGGCGA GATCCGCCAG TGCTGGGCGG AGCTGGAGAG CACCACCCAG
GCCAAGGCAC GGCAGCTCTT TGAGGCCAGC AAAGCAGACC AGCTGGTGCA GAGCTTTGCT
GAGCTGGACA AGAAGCTCCT TCACATGGAG AGCCAGCTGC AAGACGTGGA CCCTGGAGGA
GACCTGGCCA CTGTCAACAG TCAGCTCAAG AAGCTGCAGT CCATGGAGTC GCAGGTGGAG
GAGTGGTACC GCGAGGTGGG AGAGCTGCAG GCGCAGACGG CGGCGCTGCC GCTGGAGCCG
GCGAGCAAGG AGCTGGTGGG TGAGCGGCAG AACGCGGTGG GCGAGCGCCT GGTGCGCCTG
CTCGAGCCGT TGCAGGAGCG CCGCCGCTTG CTGCTGGCTT CCAAGGAGTT GCACCAGGTG
GCGCACGACC TGGACGACGA GCTGGCATGG GTTCAGGAGC GGCTGCCACT GGCCATGCAG
ACAGAGCGAG GCAACGGTTT GCAGGCGGTC CAGCAGCACA TCAAAAAGAA CCAGGGCCTG
CGGCGGGAGA TCCAGGCGCA TGGGCCGCGC CTGGAGGAGG TGCTGGAGCG CGCGGGCGCG
CTGGCGTCGC TGCGCAGCCC GGAGGCAGAG GCAGTGCGCC GGGGCCTGGA GCAGCTGCAG
AGCGCCTGGG CCGGACTGCG GGAGGCTGCC GAGCGACGGC AGCAGGTGCT GGACGCCGCC
TTCCAGGTGG AGCAGTACTA CTTCGACGTG GCTGAGGTGG AGGCGTGGCT GGGCGAGCAG
GAGCTGCTCA TGATGAGTGA GGACAAGGGC AAGGACGAAC AGAGCACCCT GCAGCTGCTC
AAGAAACACC TGCAGCTGGA GCAAGGCGTG GAGAACTACG AGGAAAGCAT CGCGCAGCTG
TCGCGCCAGT GCCGGGCGCT GCTGGAGATG GGGCACCCGG ACAGCGAGCA GATCAGCCGG
CGGCAGTCTC AGGTGGACCG CCTGTACGTG GCGCTCAAGG AGCTGGGTGA GGAGCGCCGG
GTGGCTCTGG AACAGCAGTA CTGGCTGTAC CAGCTCAGCC GCCAGGTGAG CGAGCTTGAG
CACTGGATTG CCGAGAAGGA GGTGGTGGCT GGCTCACCCG AGCTCGGCCA GGACTTTGAG
CATGTCTCGG TGCTGCAGGA GAAATTCTCA GAGTTTGCCA GCGAGACAGG TATGGCAGGG
CGGGAACGGC TGGCAGCTGT GAACCAGATG GTGGATGAGC TGATCGAGTG TGGCCATACA
GCAGCGGCCA CCATGGCCGA GTGGAAGGAC GGACTGAACG AGGCCTGGGC TGAGCTGCTG
GAGCTCATGG GCACACGGGC CCAGCTGCTG GCCGCCTCTC GGGAGCTTCA TAAGTTCTTC
AGTGACGCCC GAGAGCTTCA GGGACAGATT GAGGAGAAGC GGAGGCGGCT GCCCCGCCTG
ACCACCCCGC CTGAGCCGAG ACCCAGTGCC AGTTCCATGC AGCGGACCCT GAGAGCCTTT
GAGCATGACC TGCAGCTCCT CGTGTCCCAG GTACGGCAGC TGCAGGAGGG GGCGGCCCAG
CTGCGGACGG TGTATGCGGG TGAACATGCC GAGGCCATCG CTAGCCGGGA GCAGGAGGTG
CTGCAGGGTT GGAAAGAGCT GCTGTCAGCC TGTGAGGATG CCCGCCTGCA TGTCAGCTCC
ACAGCCGACG CCCTGCGCTT CCACAGCCAA GTCCGCGACC TGCTCTCCTG GATGGATGGC
ATCGCCAGCC AGATTGGGGC AGCCGACAAG CCCAGGGACG TGTCATCAGT GGAGGTGCTC
ATGAACTACC ACCAGGGCCT GAAGACTGAG CTGGAGGCGC GGGTGCCTGA GCTGACCACC
TGCCAGGAGC TGGGGCGATC TCTGCTGCTC AACAAAAGTG CCATGGCTGA TGAGATCCAG
GCACAGCTGG ACAAGCTGGG AACCAGGAAG GAGGAGGTGT CGGAAAAGTG GGACCGCCAT
TGGGAGTGGC TGCAGCAGAT GCTGGAGGTG CACCAGTTTG CCCAGGAGGC GGTGGTGGCT
GATGCCTGGC TGACAGCCCA GGAGCCGCTC CTGCAGAGCC GGGAGCTGGG CAGCAGCGTG
GATGAGGTGG AGCAGCTTAT CCGGCGACAT GAGGCCTTCC GCAAAGCGGC TGCAGCCTGG
GAAGAGAGGT TCAGCTCTCT GCGGCGCCTG ACCACGATCG AGAAAATCAA AGCGGAACAG
AGCAAGCAGC CGCCTACCCC ACTGCTGGGG CGCAAGTTCT TTGGGGACCC CACGGAACTG
GCGGCCAAGG CGGCGCCCCT GCTGCGGCCA GGGGGCTATG AAAGGGGCTT GGAGCCCCTG
GCCCGCCGAG CCTCGGACAC GCTCTCGGCC GAGGTGCGGA CTCGGGTGGG GTATGTGCGC
CAGGAGCTCA AGCCCGAGCG CCTCCAGCCG CGCATTGACC GGCTGCCGGA GATCCCGGGG
AGGGTGGAGC CCGCGGCCCT GCCGGCCGCA CCAGAGGACG CGGCGGAGAC CCCCGCGACC
CCCGCGGCGG CGGAGCAGGT GCGGCCACGA CCGGAGCGCC AGGAGTCAGC TGATCGCGCG
GAGGAGCTGC CCAGGAGGCG GCGGCCTGAG CGGCAAGAGT CAGTCGATCA ATCCGAGGAG
GCTGCGCGGA GGCGGCGGCC GGAGCGGCAG GAGTCAGCGG AGCACGAGGC GGCACACAGC
CTTACCCTGG GCCGCTATGA GCAGATGGAG CGGCGGCGCG AGCGGCGTGA GCGGCGCTTG
GAGCGGCAGG AGTCCAGCGA ACAGGAGATG CCCATCAGAG GAGACCTGGT CAAGGGGAAG
GCCACCCTGG CTGACATTGT GGAACAGCTG CAGGAGAAAG AGGCAGGCCC AGGGCTGCCT
GCTGGGGTCG TGGGTGAGCC TGTACTGTGT GCTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011525475.1
CDS155..7210
Misc Feature(1)338..649(+)
Misc Feature(2)338..643(+)
Misc Feature(3)695..1006(+)
Misc Feature(4)695..1003(+)
Misc Feature(5)1085..1411(+)
Misc Feature(6)1442..2086(+)
Misc Feature(7)1754..1771(+)
Misc Feature(8)2477..3115(+)
Misc Feature(9)2792..2809(+)
Misc Feature(10)3425..3739(+)
Misc Feature(11)3755..4381(+)
Misc Feature(12)4064..4081(+)
Misc Feature(13)4070..4696(+)
Misc Feature(14)4379..4396(+)
Misc Feature(15)4700..5335(+)
Misc Feature(16)5012..5029(+)
Misc Feature(17)5018..5656(+)
Misc Feature(18)5330..5347(+)
Misc Feature(19)5654..5953(+)
Misc Feature(20)5981..>6484(+)
Misc Feature(21)6293..6310(+)
Translation

Target ORF information:

RefSeq Version XM_011527173
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens spectrin, beta, non-erythrocytic 4 (SPTBN4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011527173

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGCC TGCCTGCTCC TAACAACAAC 
CCTGCTGCCC GCTGGGAGAG TCCGGATCGG GGCTGGGAGC GGGAGCAGCC GGCTGCGTCC
ACCGCAGCGG CCTCGCTCTT TGAGTGCTCC CGGATCAAGG CCTTGGCAGA TGAGCGGGAA
GCCGTGCAGA AGAAAACCTT CACCAAGTGG GTGAACTCGC ACCTCGCCCG CGTGGGCTGC
CACATCGGGG ACCTCTATGT GGACCTCCGG GACGGCTTCG TGCTCACGCG GCTCCTGGAA
GTGCTGTCTG GGGAGCAGCT GCCCAGGCCC ACGCGCGGCC GCATGCGGAT CCACTCACTG
GAGAACGTGG ACAAGGCGCT GCAGTTTCTG AAGGAGCAGC GCGTGCACCT GGAGAACGTG
GGTTCGCATG ACATCGTGGA TGGGAATCAC CGGCTGACGC TGGGGCTGGT CTGGACCATC
ATCCTGCGCT TCCAGATTCA AGTCATCAAA ATTGAGACTG AGGACAACAG AGAGACACGC
TCAGCCAAGG ATGCTCTGCT CTTGTGGTGT CAGATGAAGA CAGCTGGTTA CCCTGAGGTA
AACATCCAGA ATTTCACCAC CAGCTGGCGG GATGGCTTGG CCTTCAATGC CCTCATTCAC
CGGCACAGGC CTGATCTCGT GGACTTCAGC AAACTCACCA AGTCCAATGC CAACTACAAC
CTGCAGAGAG CCTTCCGCAC AGCTGAGCAG CACCTGGGGC TGGCGCGGCT GCTGGATCCT
GAAGATGTGA ACATGGAGGC TCCAGATGAG AAGTCCATCA TCACCTACGT GGTCTCTTTC
TACCACTATT TCTCCAAGAT GAAGGCTCTG GCTGTGGAGG GGAAGCGTAT CGGGAAGGTC
TTGGACCAGG TATTGGAGGT GGGGAAGATC ATAGAACGCT ACGAGGAGCT GGCGGCTGAG
CTGCTGGCCT GGATCCACCG CACCGTGGGC CTCATCAGCA ATCAGAAATT TGCCAACTCC
TTAAGTGGGG TGCAGCAGCA ACTCCAGGCT TTCACGGCCT ATTGCACGCT GGAGAAGCCT
GTCAAGTTCC AGGAGAAGGG GAACCTAGAG GTGCTGCTCT TCAGCATCCA GAGCAAACTG
CGTGCCTGCA ACCGTCGCCT CTTTGTGCCT CGGGAGGGCT GTGGCATCTG GGATATTGAC
AAGGCATGGG GTGAGCTGGA GAAGGCTGAG CATGAGCGGG AGGCTGCCCT ACGGGCTGAG
CTGATTCGGC AGGAGAAGCT GGAACTACTG GCACAGAGGT TTGACCACAA GGTGGCTATG
AGGGAGAGCT GGCTGAATGA GAACCAGCGT CTGGTCTCCC AGGACAACTT TGGGTATGAG
CTGCCCGCAG TGGAGGCAGC CATGAAGAAA CACGAAGCGA TCGAGGCAGA CATTGCGGCC
TACGAGGAGC GGGTGCAGGG TGTGGCGGAG CTGGCCCAGG CATTGGCAGC CGAAGGCTAC
TACGATATCC GGCGGGTGGC AGCCCAGCGT GACAGCGTCC TGCGCCAGTG GGCCCTGCTA
ACTGGGCTTG TGGGTGCCCG GCGGACACGA CTTGAGCAGA ACCTTGCCCT GCAGAAGGTC
TTCCAGGAGA TGGTGTACAT GGTGGACTGG ATGGAGGAGA TGCAGGCTCA GCTGCTGTCC
CGGGAGTGTG GGCAGCACCT GGTGGAGGCA GACGACCTGT TGCAGAAGCA TGGACTGCTG
GAGGGAGACA TTGCCGCCCA GAGCGAGCGG GTGGAGGCTC TCAATGCCGC TGCCCTGCGC
TTCTCCCAGC TGCAGGGCTA CCAGCCCTGC GACCCGCAGG TCATCTGCAA CCGCGTGAAC
CACGTGCACG GCTGCCTGGC GGAGCTGCAG GAGCAGGCAG CGCGGCGACG CGCGGAGCTG
GAGGCTTCGC GGAGCCTGTG GGCGCTGCTG CAGGAGCTGG AGGAGGCCGA GAGCTGGGCG
CGCGACAAGG AGCGTCTCCT GGAGGCTGCG GGCGGCGGCG GTGCGGCGGG CGCAGCGGGC
GCAGCGGGAA CAGCGGGCGG CGCGCATGAC CTGTCCAGCA CAGCGCGCCT CCTGGCCCAG
CACAAGATCC TGCAGGGCGA GCTGGGCGGG CGGCGAGCGT TGCTGCAGCA GGCCCTGCGG
TGTGGCGAGG AGCTGGTTGC GGCCGGCGGT GCCGTCGGCC CGGGAGCAGA CACCGTGCAC
CTGGTAGGCC TGGCGGAGCG CGCGGCGAGC GCCCGGCGCC GCTGGCAGAG GCTGGAAGAG
GCGGCGGCGC GGCGAGAGCG GCGGCTGCAG GAGGCGCGGG CGCTGCACCA GTTCGGCGCT
GACCTCGACG GGCTGCTGGA CTGGCTTCGC GACGCTTACC GCCTGGCAGC CGCCGGTGAC
TTCGGCCACG ACGAAGCTTC CAGCCGCCGC CTGGCGCGCC AGCACCGCGC GCTCACCGGG
GAGGTGGAGG CACATCGCGG GCCCGTGAGC GGCCTGCGGC GCCAGCTGGC GACACTCGGG
GGTGCCAGTG GCGCAGGGCC ACTGGTGGTG GCGCTGCAGG TGCGCGTGGT GGAAGCAGAG
CAGTTGTTCG CTGAGGTGAC CGAAGTGGCG GCGCTGAGGC GCCAGTGGCT GCGGGACGCG
CTCGCTGTCT ACCGCATGTT TGGCGAGGTG CACGCGTGTG AGCTGTGGAT CGGCGAGAAG
GAGCAATGGC TGCTCTCCAT GCGTGTGCCG GATTCACTCG ACGACGTCGA GGTGGTGCAG
CACCGATTCG AGAGCCTGGA CCAAGAGATG AACAGCCTGA TGGGCCGCGT TCTGGACGTG
AACCACACAG TCCAGGAGCT GGTGGAAGGA GGCCACCCCA GTTCAGATGA GGTGCGTTCC
TGCCAGGACC ACCTCAACAG CAGGTGGAAC CGCATCGTGG AGCTAGTGGA ACAGCGCAAA
GAGGAAATGA GCGCGGTGCT GCTGGTGGAG AACCACGTGC TGGAGGTGGC CGAGGTGCGC
GCCCAGGTGC GTGAGAAGCG GAGAGCTGTG GAGAGCGCGC CCCGGGCCGG CGGCGCCCTG
CAGTGGCGTC TTAGCGGCCT AGAGGCCGCT CTGCAGGCGC TGGAGCCGCG CCAGGCGGCC
CTTCTGGAGG AGGCAGCCCT GCTGGCTGAG CGCTTCCCGG CGCAGGCGGC GCGGCTGCAC
CAGGGCGCGG AGGAGCTGGG CGCCGAGTGG GGCGCGCTAG CTAGCGCGGC TCAGGCCTGC
GGCGAGGCGG TGGCGGCAGC AGGGCGCCTG CAGCGCTTCC TACATGACCT CGACGCTTTC
CTGGACTGGC TCGTGCGCGC CCAGGAGGCG GCGGGCGGCA GCGAGGGGCC CCTGCCCAAC
AGCCTAGAAG AGGCGGACGC GCTGCTGGCG CGCCACGCTG CGCTCAAGGA GGAGGTGGAC
CAGCGCGAGG AAGACTATGC TCGCATCGTG GCGGCCAGCG AGGCGCTGCT GGCCGCCGAC
GGCGCAGAGC TGGGCCCGGG CCTGGCACTA GACGAGTGGC TGCCACACCT CGAACTTGGC
TGGCATAAAC TGCTCGGCTT GTGGGAGGCG CGCAGGGAGG CGCTGGTCCA GGCGCACATC
TACCAGCTCT TCCTGCGGGA TCTACGCCAG GCGCTCGTGG TGCTGCGTAA CCAGGAGATG
GCGCTGTCTG GTGCGGAGCT CCCGGGCACA GTGGAATCGG TGGAGGAGGC CTTGAAACAG
CACCGTGACT TTCTCACCAC CATGGAGCTG AGCCAGCAAA AGATGCAGGT GGCCGTGCAG
GCTGCAGAGG GCCTGCTGAG GCAGGGCAAC ATCTACGGGG AGCAGGCTCA GGAGGCTGTG
ACCCGGCTGC TGGAGAAGAA CCAAGAAAAC CAGTTACGGG CCCAGCAATG GATGCAAAAG
CTACATGACC AACTTGAGCT GCAGCACTTC CTCCGAGACT GCCACGAGCT GGATGGCTGG
ATCCATGAGA AGATGCTGAT GGCGCGGGAT GGCACGCGGG AGGACAACCA CAAGCTGCAT
AAGAGATGGC TCCGGCACCA GGCATTCATG GCCGAGCTGG CTCAGAATAA GGAGTGGCTG
GAGAAGATCG AGCGGGAGGG CCAGCAACTG ATGCAGGAGA AGCCCGAACT GGCGGCCTCC
GTGCGGAAGA AGCTGGGCGA GATCCGCCAG TGCTGGGCGG AGCTGGAGAG CACCACCCAG
GCCAAGGCAC GGCAGCTCTT TGAGGCCAGC AAAGCAGACC AGCTGGTGCA GAGCTTTGCT
GAGCTGGACA AGAAGCTCCT TCACATGGAG AGCCAGCTGC AAGACGTGGA CCCTGGAGGA
GACCTGGCCA CTGTCAACAG TCAGCTCAAG AAGCTGCAGT CCATGGAGTC GCAGGTGGAG
GAGTGGTACC GCGAGGTGGG AGAGCTGCAG GCGCAGACGG CGGCGCTGCC GCTGGAGCCG
GCGAGCAAGG AGCTGGTGGG TGAGCGGCAG AACGCGGTGG GCGAGCGCCT GGTGCGCCTG
CTCGAGCCGT TGCAGGAGCG CCGCCGCTTG CTGCTGGCTT CCAAGGAGTT GCACCAGGTG
GCGCACGACC TGGACGACGA GCTGGCATGG GTTCAGGAGC GGCTGCCACT GGCCATGCAG
ACAGAGCGAG GCAACGGTTT GCAGGCGGTC CAGCAGCACA TCAAAAAGAA CCAGGGCCTG
CGGCGGGAGA TCCAGGCGCA TGGGCCGCGC CTGGAGGAGG TGCTGGAGCG CGCGGGCGCG
CTGGCGTCGC TGCGCAGCCC GGAGGCAGAG GCAGTGCGCC GGGGCCTGGA GCAGCTGCAG
AGCGCCTGGG CCGGACTGCG GGAGGCTGCC GAGCGACGGC AGCAGGTGCT GGACGCCGCC
TTCCAGGTGG AGCAGTACTA CTTCGACGTG GCTGAGGTGG AGGCGTGGCT GGGCGAGCAG
GAGCTGCTCA TGATGAGTGA GGACAAGGGC AAGGACGAAC AGAGCACCCT GCAGCTGCTC
AAGAAACACC TGCAGCTGGA GCAAGGCGTG GAGAACTACG AGGAAAGCAT CGCGCAGCTG
TCGCGCCAGT GCCGGGCGCT GCTGGAGATG GGGCACCCGG ACAGCGAGCA GATCAGCCGG
CGGCAGTCTC AGGTGGACCG CCTGTACGTG GCGCTCAAGG AGCTGGGTGA GGAGCGCCGG
GTGGCTCTGG AACAGCAGTA CTGGCTGTAC CAGCTCAGCC GCCAGGTGAG CGAGCTTGAG
CACTGGATTG CCGAGAAGGA GGTGGTGGCT GGCTCACCCG AGCTCGGCCA GGACTTTGAG
CATGTCTCGG TGCTGCAGGA GAAATTCTCA GAGTTTGCCA GCGAGACAGG TATGGCAGGG
CGGGAACGGC TGGCAGCTGT GAACCAGATG GTGGATGAGC TGATCGAGTG TGGCCATACA
GCAGCGGCCA CCATGGCCGA GTGGAAGGAC GGACTGAACG AGGCCTGGGC TGAGCTGCTG
GAGCTCATGG GCACACGGGC CCAGCTGCTG GCCGCCTCTC GGGAGCTTCA TAAGTTCTTC
AGTGACGCCC GAGAGCTTCA GGGACAGATT GAGGAGAAGC GGAGGCGGCT GCCCCGCCTG
ACCACCCCGC CTGAGCCGAG ACCCAGTGCC AGTTCCATGC AGCGGACCCT GAGAGCCTTT
GAGCATGACC TGCAGCTCCT CGTGTCCCAG GTACGGCAGC TGCAGGAGGG GGCGGCCCAG
CTGCGGACGG TGTATGCGGG TGAACATGCC GAGGCCATCG CTAGCCGGGA GCAGGAGGTG
CTGCAGGGTT GGAAAGAGCT GCTGTCAGCC TGTGAGGATG CCCGCCTGCA TGTCAGCTCC
ACAGCCGACG CCCTGCGCTT CCACAGCCAA GTCCGCGACC TGCTCTCCTG GATGGATGGC
ATCGCCAGCC AGATTGGGGC AGCCGACAAG CCCAGGGACG TGTCATCAGT GGAGGTGCTC
ATGAACTACC ACCAGGGCCT GAAGACTGAG CTGGAGGCGC GGGTGCCTGA GCTGACCACC
TGCCAGGAGC TGGGGCGATC TCTGCTGCTC AACAAAAGTG CCATGGCTGA TGAGATCCAG
GCACAGCTGG ACAAGCTGGG AACCAGGAAG GAGGAGGTGT CGGAAAAGTG GGACCGCCAT
TGGGAGTGGC TGCAGCAGAT GCTGGAGGTG CACCAGTTTG CCCAGGAGGC GGTGGTGGCT
GATGCCTGGC TGACAGCCCA GGAGCCGCTC CTGCAGAGCC GGGAGCTGGG CAGCAGCGTG
GATGAGGTGG AGCAGCTTAT CCGGCGACAT GAGGCCTTCC GCAAAGCGGC TGCAGCCTGG
GAAGAGAGGT TCAGCTCTCT GCGGCGCCTG ACCACGATCG AGAAAATCAA AGCGGAACAG
AGCAAGCAGC CGCCTACCCC ACTGCTGGGG CGCAAGTTCT TTGGGGACCC CACGGAACTG
GCGGCCAAGG CGGCGCCCCT GCTGCGGCCA GGGGGCTATG AAAGGGGCTT GGAGCCCCTG
GCCCGCCGAG CCTCGGACAC GCTCTCGGCC GAGGTGCGGA CTCGGGTGGG GTATGTGCGC
CAGGAGCTCA AGCCCGAGCG CCTCCAGCCG CGCATTGACC GGCTGCCGGA GATCCCGGGG
AGGGTGGAGC CCGCGGCCCT GCCGGCCGCA CCAGAGGACG CGGCGGAGAC CCCCGCGACC
CCCGCGGCGG CGGAGCAGGT GCGGCCACGA CCGGAGCGCC AGGAGTCAGC TGATCGCGCG
GAGGAGCTGC CCAGGAGGCG GCGGCCTGAG CGGCAAGAGT CAGTCGATCA ATCCGAGGAG
GCTGCGCGGA GGCGGCGGCC GGAGCGGCAG GAGTCAGCGG AGCACGAGGC GGCACACAGC
CTTACCCTGG GCCGCTATGA GCAGATGGAG CGGCGGCGCG AGCGGCGTGA GCGGCGCTTG
GAGCGGCAGG AGTCCAGCGA ACAGGAGATG CCCATCAGAG GAGACCTGGT CAAGGGGAAG
GCCACCCTGG CTGACATTGT GGAACAGCTG CAGGAGAAAG AGGCAGGCCC AGGGCTGCCT
GCTGGGGTCG TGGGTGAGCC TGTACTGTGT GCTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19524
Clone ID Related Accession (Same CDS sequence) NM_020971 , XM_011527172
Accession Version NM_020971.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 7695bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product spectrin beta chain, non-erythrocytic 4 isoform sigma1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF311855.1, AC020929.6, AB046862.1 and AI962525.1. This sequence is a reference standard in the RefSeqGene project. On Sep 30, 2006 this sequence version replaced gi:40353203. Transcript Variant: This variant (sigma1) represents the longer transcript and encodes the longer isoform (sigma1), also known as the 289 kDa isoform. ##Evidence-Data-START## Transcript exon combination :: AF311855.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGCC TGCCTGCTCC TAACAACAAC 
CCTGCTGCCC GCTGGGAGAG TCCGGATCGG GGCTGGGAGC GGGAGCAGCC GGCTGCGTCC
ACCGCAGCGG CCTCGCTCTT TGAGTGCTCC CGGATCAAGG CCTTGGCAGA TGAGCGGGAA
GCCGTGCAGA AGAAAACCTT CACCAAGTGG GTGAACTCGC ACCTCGCCCG CGTGGGCTGC
CACATCGGGG ACCTCTATGT GGACCTCCGG GACGGCTTCG TGCTCACGCG GCTCCTGGAA
GTGCTGTCTG GGGAGCAGCT GCCCAGGCCC ACGCGCGGCC GCATGCGGAT CCACTCACTG
GAGAACGTGG ACAAGGCGCT GCAGTTTCTG AAGGAGCAGC GCGTGCACCT GGAGAACGTG
GGTTCGCATG ACATCGTGGA TGGGAATCAC CGGCTGACGC TGGGGCTGGT CTGGACCATC
ATCCTGCGCT TCCAGATTCA AGTCATCAAA ATTGAGACTG AGGACAACAG AGAGACACGC
TCAGCCAAGG ATGCTCTGCT CTTGTGGTGT CAGATGAAGA CAGCTGGTTA CCCTGAGGTA
AACATCCAGA ATTTCACCAC CAGCTGGCGG GATGGCTTGG CCTTCAATGC CCTCATTCAC
CGGCACAGGC CTGATCTCGT GGACTTCAGC AAACTCACCA AGTCCAATGC CAACTACAAC
CTGCAGAGAG CCTTCCGCAC AGCTGAGCAG CACCTGGGGC TGGCGCGGCT GCTGGATCCT
GAAGATGTGA ACATGGAGGC TCCAGATGAG AAGTCCATCA TCACCTACGT GGTCTCTTTC
TACCACTATT TCTCCAAGAT GAAGGCTCTG GCTGTGGAGG GGAAGCGTAT CGGGAAGGTC
TTGGACCAGG TATTGGAGGT GGGGAAGATC ATAGAACGCT ACGAGGAGCT GGCGGCTGAG
CTGCTGGCCT GGATCCACCG CACCGTGGGC CTCATCAGCA ATCAGAAATT TGCCAACTCC
TTAAGTGGGG TGCAGCAGCA ACTCCAGGCT TTCACGGCCT ATTGCACGCT GGAGAAGCCT
GTCAAGTTCC AGGAGAAGGG GAACCTAGAG GTGCTGCTCT TCAGCATCCA GAGCAAACTG
CGTGCCTGCA ACCGTCGCCT CTTTGTGCCT CGGGAGGGCT GTGGCATCTG GGATATTGAC
AAGGCATGGG GTGAGCTGGA GAAGGCTGAG CATGAGCGGG AGGCTGCCCT ACGGGCTGAG
CTGATTCGGC AGGAGAAGCT GGAACTACTG GCACAGAGGT TTGACCACAA GGTGGCTATG
AGGGAGAGCT GGCTGAATGA GAACCAGCGT CTGGTCTCCC AGGACAACTT TGGGTATGAG
CTGCCCGCAG TGGAGGCAGC CATGAAGAAA CACGAAGCGA TCGAGGCAGA CATTGCGGCC
TACGAGGAGC GGGTGCAGGG TGTGGCGGAG CTGGCCCAGG CATTGGCAGC CGAAGGCTAC
TACGATATCC GGCGGGTGGC AGCCCAGCGT GACAGCGTCC TGCGCCAGTG GGCCCTGCTA
ACTGGGCTTG TGGGTGCCCG GCGGACACGA CTTGAGCAGA ACCTTGCCCT GCAGAAGGTC
TTCCAGGAGA TGGTGTACAT GGTGGACTGG ATGGAGGAGA TGCAGGCTCA GCTGCTGTCC
CGGGAGTGTG GGCAGCACCT GGTGGAGGCA GACGACCTGT TGCAGAAGCA TGGACTGCTG
GAGGGAGACA TTGCCGCCCA GAGCGAGCGG GTGGAGGCTC TCAATGCCGC TGCCCTGCGC
TTCTCCCAGC TGCAGGGCTA CCAGCCCTGC GACCCGCAGG TCATCTGCAA CCGCGTGAAC
CACGTGCACG GCTGCCTGGC GGAGCTGCAG GAGCAGGCAG CGCGGCGACG CGCGGAGCTG
GAGGCTTCGC GGAGCCTGTG GGCGCTGCTG CAGGAGCTGG AGGAGGCCGA GAGCTGGGCG
CGCGACAAGG AGCGTCTCCT GGAGGCTGCG GGCGGCGGCG GTGCGGCGGG CGCAGCGGGC
GCAGCGGGAA CAGCGGGCGG CGCGCATGAC CTGTCCAGCA CAGCGCGCCT CCTGGCCCAG
CACAAGATCC TGCAGGGCGA GCTGGGCGGG CGGCGAGCGT TGCTGCAGCA GGCCCTGCGG
TGTGGCGAGG AGCTGGTTGC GGCCGGCGGT GCCGTCGGCC CGGGAGCAGA CACCGTGCAC
CTGGTAGGCC TGGCGGAGCG CGCGGCGAGC GCCCGGCGCC GCTGGCAGAG GCTGGAAGAG
GCGGCGGCGC GGCGAGAGCG GCGGCTGCAG GAGGCGCGGG CGCTGCACCA GTTCGGCGCT
GACCTCGACG GGCTGCTGGA CTGGCTTCGC GACGCTTACC GCCTGGCAGC CGCCGGTGAC
TTCGGCCACG ACGAAGCTTC CAGCCGCCGC CTGGCGCGCC AGCACCGCGC GCTCACCGGG
GAGGTGGAGG CACATCGCGG GCCCGTGAGC GGCCTGCGGC GCCAGCTGGC GACACTCGGG
GGTGCCAGTG GCGCAGGGCC ACTGGTGGTG GCGCTGCAGG TGCGCGTGGT GGAAGCAGAG
CAGTTGTTCG CTGAGGTGAC CGAAGTGGCG GCGCTGAGGC GCCAGTGGCT GCGGGACGCG
CTCGCTGTCT ACCGCATGTT TGGCGAGGTG CACGCGTGTG AGCTGTGGAT CGGCGAGAAG
GAGCAATGGC TGCTCTCCAT GCGTGTGCCG GATTCACTCG ACGACGTCGA GGTGGTGCAG
CACCGATTCG AGAGCCTGGA CCAAGAGATG AACAGCCTGA TGGGCCGCGT TCTGGACGTG
AACCACACAG TCCAGGAGCT GGTGGAAGGA GGCCACCCCA GTTCAGATGA GGTGCGTTCC
TGCCAGGACC ACCTCAACAG CAGGTGGAAC CGCATCGTGG AGCTAGTGGA ACAGCGCAAA
GAGGAAATGA GCGCGGTGCT GCTGGTGGAG AACCACGTGC TGGAGGTGGC CGAGGTGCGC
GCCCAGGTGC GTGAGAAGCG GAGAGCTGTG GAGAGCGCGC CCCGGGCCGG CGGCGCCCTG
CAGTGGCGTC TTAGCGGCCT AGAGGCCGCT CTGCAGGCGC TGGAGCCGCG CCAGGCGGCC
CTTCTGGAGG AGGCAGCCCT GCTGGCTGAG CGCTTCCCGG CGCAGGCGGC GCGGCTGCAC
CAGGGCGCGG AGGAGCTGGG CGCCGAGTGG GGCGCGCTAG CTAGCGCGGC TCAGGCCTGC
GGCGAGGCGG TGGCGGCAGC AGGGCGCCTG CAGCGCTTCC TACATGACCT CGACGCTTTC
CTGGACTGGC TCGTGCGCGC CCAGGAGGCG GCGGGCGGCA GCGAGGGGCC CCTGCCCAAC
AGCCTAGAAG AGGCGGACGC GCTGCTGGCG CGCCACGCTG CGCTCAAGGA GGAGGTGGAC
CAGCGCGAGG AAGACTATGC TCGCATCGTG GCGGCCAGCG AGGCGCTGCT GGCCGCCGAC
GGCGCAGAGC TGGGCCCGGG CCTGGCACTA GACGAGTGGC TGCCACACCT CGAACTTGGC
TGGCATAAAC TGCTCGGCTT GTGGGAGGCG CGCAGGGAGG CGCTGGTCCA GGCGCACATC
TACCAGCTCT TCCTGCGGGA TCTACGCCAG GCGCTCGTGG TGCTGCGTAA CCAGGAGATG
GCGCTGTCTG GTGCGGAGCT CCCGGGCACA GTGGAATCGG TGGAGGAGGC CTTGAAACAG
CACCGTGACT TTCTCACCAC CATGGAGCTG AGCCAGCAAA AGATGCAGGT GGCCGTGCAG
GCTGCAGAGG GCCTGCTGAG GCAGGGCAAC ATCTACGGGG AGCAGGCTCA GGAGGCTGTG
ACCCGGCTGC TGGAGAAGAA CCAAGAAAAC CAGTTACGGG CCCAGCAATG GATGCAAAAG
CTACATGACC AACTTGAGCT GCAGCACTTC CTCCGAGACT GCCACGAGCT GGATGGCTGG
ATCCATGAGA AGATGCTGAT GGCGCGGGAT GGCACGCGGG AGGACAACCA CAAGCTGCAT
AAGAGATGGC TCCGGCACCA GGCATTCATG GCCGAGCTGG CTCAGAATAA GGAGTGGCTG
GAGAAGATCG AGCGGGAGGG CCAGCAACTG ATGCAGGAGA AGCCCGAACT GGCGGCCTCC
GTGCGGAAGA AGCTGGGCGA GATCCGCCAG TGCTGGGCGG AGCTGGAGAG CACCACCCAG
GCCAAGGCAC GGCAGCTCTT TGAGGCCAGC AAAGCAGACC AGCTGGTGCA GAGCTTTGCT
GAGCTGGACA AGAAGCTCCT TCACATGGAG AGCCAGCTGC AAGACGTGGA CCCTGGAGGA
GACCTGGCCA CTGTCAACAG TCAGCTCAAG AAGCTGCAGT CCATGGAGTC GCAGGTGGAG
GAGTGGTACC GCGAGGTGGG AGAGCTGCAG GCGCAGACGG CGGCGCTGCC GCTGGAGCCG
GCGAGCAAGG AGCTGGTGGG TGAGCGGCAG AACGCGGTGG GCGAGCGCCT GGTGCGCCTG
CTCGAGCCGT TGCAGGAGCG CCGCCGCTTG CTGCTGGCTT CCAAGGAGTT GCACCAGGTG
GCGCACGACC TGGACGACGA GCTGGCATGG GTTCAGGAGC GGCTGCCACT GGCCATGCAG
ACAGAGCGAG GCAACGGTTT GCAGGCGGTC CAGCAGCACA TCAAAAAGAA CCAGGGCCTG
CGGCGGGAGA TCCAGGCGCA TGGGCCGCGC CTGGAGGAGG TGCTGGAGCG CGCGGGCGCG
CTGGCGTCGC TGCGCAGCCC GGAGGCAGAG GCAGTGCGCC GGGGCCTGGA GCAGCTGCAG
AGCGCCTGGG CCGGACTGCG GGAGGCTGCC GAGCGACGGC AGCAGGTGCT GGACGCCGCC
TTCCAGGTGG AGCAGTACTA CTTCGACGTG GCTGAGGTGG AGGCGTGGCT GGGCGAGCAG
GAGCTGCTCA TGATGAGTGA GGACAAGGGC AAGGACGAAC AGAGCACCCT GCAGCTGCTC
AAGAAACACC TGCAGCTGGA GCAAGGCGTG GAGAACTACG AGGAAAGCAT CGCGCAGCTG
TCGCGCCAGT GCCGGGCGCT GCTGGAGATG GGGCACCCGG ACAGCGAGCA GATCAGCCGG
CGGCAGTCTC AGGTGGACCG CCTGTACGTG GCGCTCAAGG AGCTGGGTGA GGAGCGCCGG
GTGGCTCTGG AACAGCAGTA CTGGCTGTAC CAGCTCAGCC GCCAGGTGAG CGAGCTTGAG
CACTGGATTG CCGAGAAGGA GGTGGTGGCT GGCTCACCCG AGCTCGGCCA GGACTTTGAG
CATGTCTCGG TGCTGCAGGA GAAATTCTCA GAGTTTGCCA GCGAGACAGG TATGGCAGGG
CGGGAACGGC TGGCAGCTGT GAACCAGATG GTGGATGAGC TGATCGAGTG TGGCCATACA
GCAGCGGCCA CCATGGCCGA GTGGAAGGAC GGACTGAACG AGGCCTGGGC TGAGCTGCTG
GAGCTCATGG GCACACGGGC CCAGCTGCTG GCCGCCTCTC GGGAGCTTCA TAAGTTCTTC
AGTGACGCCC GAGAGCTTCA GGGACAGATT GAGGAGAAGC GGAGGCGGCT GCCCCGCCTG
ACCACCCCGC CTGAGCCGAG ACCCAGTGCC AGTTCCATGC AGCGGACCCT GAGAGCCTTT
GAGCATGACC TGCAGCTCCT CGTGTCCCAG GTACGGCAGC TGCAGGAGGG GGCGGCCCAG
CTGCGGACGG TGTATGCGGG TGAACATGCC GAGGCCATCG CTAGCCGGGA GCAGGAGGTG
CTGCAGGGTT GGAAAGAGCT GCTGTCAGCC TGTGAGGATG CCCGCCTGCA TGTCAGCTCC
ACAGCCGACG CCCTGCGCTT CCACAGCCAA GTCCGCGACC TGCTCTCCTG GATGGATGGC
ATCGCCAGCC AGATTGGGGC AGCCGACAAG CCCAGGGACG TGTCATCAGT GGAGGTGCTC
ATGAACTACC ACCAGGGCCT GAAGACTGAG CTGGAGGCGC GGGTGCCTGA GCTGACCACC
TGCCAGGAGC TGGGGCGATC TCTGCTGCTC AACAAAAGTG CCATGGCTGA TGAGATCCAG
GCACAGCTGG ACAAGCTGGG AACCAGGAAG GAGGAGGTGT CGGAAAAGTG GGACCGCCAT
TGGGAGTGGC TGCAGCAGAT GCTGGAGGTG CACCAGTTTG CCCAGGAGGC GGTGGTGGCT
GATGCCTGGC TGACAGCCCA GGAGCCGCTC CTGCAGAGCC GGGAGCTGGG CAGCAGCGTG
GATGAGGTGG AGCAGCTTAT CCGGCGACAT GAGGCCTTCC GCAAAGCGGC TGCAGCCTGG
GAAGAGAGGT TCAGCTCTCT GCGGCGCCTG ACCACGATCG AGAAAATCAA AGCGGAACAG
AGCAAGCAGC CGCCTACCCC ACTGCTGGGG CGCAAGTTCT TTGGGGACCC CACGGAACTG
GCGGCCAAGG CGGCGCCCCT GCTGCGGCCA GGGGGCTATG AAAGGGGCTT GGAGCCCCTG
GCCCGCCGAG CCTCGGACAC GCTCTCGGCC GAGGTGCGGA CTCGGGTGGG GTATGTGCGC
CAGGAGCTCA AGCCCGAGCG CCTCCAGCCG CGCATTGACC GGCTGCCGGA GATCCCGGGG
AGGGTGGAGC CCGCGGCCCT GCCGGCCGCA CCAGAGGACG CGGCGGAGAC CCCCGCGACC
CCCGCGGCGG CGGAGCAGGT GCGGCCACGA CCGGAGCGCC AGGAGTCAGC TGATCGCGCG
GAGGAGCTGC CCAGGAGGCG GCGGCCTGAG CGGCAAGAGT CAGTCGATCA ATCCGAGGAG
GCTGCGCGGA GGCGGCGGCC GGAGCGGCAG GAGTCAGCGG AGCACGAGGC GGCACACAGC
CTTACCCTGG GCCGCTATGA GCAGATGGAG CGGCGGCGCG AGCGGCGTGA GCGGCGCTTG
GAGCGGCAGG AGTCCAGCGA ACAGGAGATG CCCATCAGAG GAGACCTGGT CAAGGGGAAG
GCCACCCTGG CTGACATTGT GGAACAGCTG CAGGAGAAAG AGGCAGGCCC AGGGCTGCCT
GCTGGGCCGT CGCTGCCTCA GCCACGCGAG CTTCCCCCAG GTCGCCTGCC CAACGGGCTT
GAGCTGCCCG AGCGGACACC TCGGCCGGAC CGGCCCCGGG CGCGGGACCG GCCCAAGCCG
CGACGGCGGC CGCGGCCCAG AGAGGGTGGT GAGGGCGGGG GAAGCCGGCG CTCGCGCTCC
GCCCCGGCCC AGGGCGGCTC CGCCCCCGCG CCTCCGCCAC CGCCCACTCA CACAGTGCAG
CACGAGGGCT TCCTACTGCG CAAGCGCGAG CTCGACGCTA ACCGCAAGTC GTCCAACCGG
TCGTGGGTGA GCCTGTACTG TGTGCTTAGT AAGGGGGAAC TGGGCTTCTA CAAGGACTCC
AAGGGCCCGG CATCCGGGAG CACACACGGT GGGGAACCGC TGCTCAGCCT GCACAAGGCC
ACCAGCGAGG TGGCTAGTGA CTACAAGAAA AAGAAGCATG TCTTCAAGCT CCAGACCCAG
GATGGCAGTG AGTTTTTGCT CCAGGCAAAA GATGAGGAGG AGATGAACGG CTGGCTGGAG
GCTGTAGCTT CCTCGGTGGC GGAACACGCA GAGATCGCCC GCTGGGGCCA GACACTACCC
ACTACTTCAT CCACAGATGA GGGCAACCCT AAGAGGGAAG GCGGAGATCG CAGGGCCAGC
GGGCGCAGGA AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_066022.2
CDS103..7797
Misc Feature(1)286..597(+)
Misc Feature(2)286..591(+)
Misc Feature(3)643..954(+)
Misc Feature(4)643..951(+)
Misc Feature(5)1027..1164(+)
Misc Feature(6)1033..1359(+)
Misc Feature(7)1294..1359(+)
Misc Feature(8)1387..1701(+)
Misc Feature(9)1390..2034(+)
Misc Feature(10)1702..1719(+)
Misc Feature(11)1705..2028(+)
Misc Feature(12)2032..2415(+)
Misc Feature(13)2419..2739(+)
Misc Feature(14)2425..3063(+)
Misc Feature(15)2740..2757(+)
Misc Feature(16)2743..3057(+)
Misc Feature(17)3157..3360(+)
Misc Feature(18)3364..3693(+)
Misc Feature(19)3373..3687(+)
Misc Feature(20)3697..4011(+)
Misc Feature(21)3703..4329(+)
Misc Feature(22)4012..4029(+)
Misc Feature(23)4015..4326(+)
Misc Feature(24)4018..4644(+)
Misc Feature(25)4327..4344(+)
Misc Feature(26)4330..4641(+)
Misc Feature(27)4645..4959(+)
Misc Feature(28)4648..5283(+)
Misc Feature(29)4960..4977(+)
Misc Feature(30)4963..5277(+)
Misc Feature(31)4966..5604(+)
Misc Feature(32)5278..5295(+)
Misc Feature(33)5281..5598(+)
Misc Feature(34)5602..5922(+)
Misc Feature(35)5602..5901(+)
Misc Feature(36)5926..6240(+)
Misc Feature(37)5929..>6432(+)
Misc Feature(38)6241..6258(+)
Misc Feature(39)6244..6423(+)
Misc Feature(40)7363..7677(+)
Misc Feature(41)7384..7566(+)
Exon (1)1..87
Gene:SPTBN4
Gene Synonym:
Exon (2)88..271
Gene:SPTBN4
Gene Synonym:
Exon (3)272..423
Gene:SPTBN4
Gene Synonym:
Exon (4)424..597
Gene:SPTBN4
Gene Synonym:
Exon (5)598..689
Gene:SPTBN4
Gene Synonym:
Exon (6)690..770
Gene:SPTBN4
Gene Synonym:
Exon (7)771..886
Gene:SPTBN4
Gene Synonym:
Exon (8)887..999
Gene:SPTBN4
Gene Synonym:
Exon (9)1000..1187
Gene:SPTBN4
Gene Synonym:
Exon (10)1188..1305
Gene:SPTBN4
Gene Synonym:
Exon (11)1306..1464
Gene:SPTBN4
Gene Synonym:
Exon (12)1465..1767
Gene:SPTBN4
Gene Synonym:
Exon (13)1768..1918
Gene:SPTBN4
Gene Synonym:
Exon (14)1919..2867
Gene:SPTBN4
Gene Synonym:
Exon (15)2868..3005
Gene:SPTBN4
Gene Synonym:
Exon (16)3006..3756
Gene:SPTBN4
Gene Synonym:
Exon (17)3757..3959
Gene:SPTBN4
Gene Synonym:
Exon (18)3960..4050
Gene:SPTBN4
Gene Synonym:
Exon (19)4051..4197
Gene:SPTBN4
Gene Synonym:
Exon (20)4198..4461
Gene:SPTBN4
Gene Synonym:
Exon (21)4462..4686
Gene:SPTBN4
Gene Synonym:
Exon (22)4687..4776
Gene:SPTBN4
Gene Synonym:
Exon (23)4777..5055
Gene:SPTBN4
Gene Synonym:
Exon (24)5056..5186
Gene:SPTBN4
Gene Synonym:
Exon (25)5187..5391
Gene:SPTBN4
Gene Synonym:
Exon (26)5392..5772
Gene:SPTBN4
Gene Synonym:
Exon (27)5773..6017
Gene:SPTBN4
Gene Synonym:
Exon (28)6018..6156
Gene:SPTBN4
Gene Synonym:
Exon (29)6157..6241
Gene:SPTBN4
Gene Synonym:
Exon (30)6242..6438
Gene:SPTBN4
Gene Synonym:
Exon (31)6439..7058
Gene:SPTBN4
Gene Synonym:
Exon (32)7059..7128
Gene:SPTBN4
Gene Synonym:
Exon (33)7129..7421
Gene:SPTBN4
Gene Synonym:
Exon (34)7422..7595
Gene:SPTBN4
Gene Synonym:
Exon (35)7596..7638
Gene:SPTBN4
Gene Synonym:
Exon (36)7639..8687
Gene:SPTBN4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_020971
Organism Homo sapiens (human)
Definition Homo sapiens spectrin, beta, non-erythrocytic 4 (SPTBN4), transcript variant sigma1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_020971

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
7261
7321
7381
7441
7501
7561
7621
7681
ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGCC TGCCTGCTCC TAACAACAAC 
CCTGCTGCCC GCTGGGAGAG TCCGGATCGG GGCTGGGAGC GGGAGCAGCC GGCTGCGTCC
ACCGCAGCGG CCTCGCTCTT TGAGTGCTCC CGGATCAAGG CCTTGGCAGA TGAGCGGGAA
GCCGTGCAGA AGAAAACCTT CACCAAGTGG GTGAACTCGC ACCTCGCCCG CGTGGGCTGC
CACATCGGGG ACCTCTATGT GGACCTCCGG GACGGCTTCG TGCTCACGCG GCTCCTGGAA
GTGCTGTCTG GGGAGCAGCT GCCCAGGCCC ACGCGCGGCC GCATGCGGAT CCACTCACTG
GAGAACGTGG ACAAGGCGCT GCAGTTTCTG AAGGAGCAGC GCGTGCACCT GGAGAACGTG
GGTTCGCATG ACATCGTGGA TGGGAATCAC CGGCTGACGC TGGGGCTGGT CTGGACCATC
ATCCTGCGCT TCCAGATTCA AGTCATCAAA ATTGAGACTG AGGACAACAG AGAGACACGC
TCAGCCAAGG ATGCTCTGCT CTTGTGGTGT CAGATGAAGA CAGCTGGTTA CCCTGAGGTA
AACATCCAGA ATTTCACCAC CAGCTGGCGG GATGGCTTGG CCTTCAATGC CCTCATTCAC
CGGCACAGGC CTGATCTCGT GGACTTCAGC AAACTCACCA AGTCCAATGC CAACTACAAC
CTGCAGAGAG CCTTCCGCAC AGCTGAGCAG CACCTGGGGC TGGCGCGGCT GCTGGATCCT
GAAGATGTGA ACATGGAGGC TCCAGATGAG AAGTCCATCA TCACCTACGT GGTCTCTTTC
TACCACTATT TCTCCAAGAT GAAGGCTCTG GCTGTGGAGG GGAAGCGTAT CGGGAAGGTC
TTGGACCAGG TATTGGAGGT GGGGAAGATC ATAGAACGCT ACGAGGAGCT GGCGGCTGAG
CTGCTGGCCT GGATCCACCG CACCGTGGGC CTCATCAGCA ATCAGAAATT TGCCAACTCC
TTAAGTGGGG TGCAGCAGCA ACTCCAGGCT TTCACGGCCT ATTGCACGCT GGAGAAGCCT
GTCAAGTTCC AGGAGAAGGG GAACCTAGAG GTGCTGCTCT TCAGCATCCA GAGCAAACTG
CGTGCCTGCA ACCGTCGCCT CTTTGTGCCT CGGGAGGGCT GTGGCATCTG GGATATTGAC
AAGGCATGGG GTGAGCTGGA GAAGGCTGAG CATGAGCGGG AGGCTGCCCT ACGGGCTGAG
CTGATTCGGC AGGAGAAGCT GGAACTACTG GCACAGAGGT TTGACCACAA GGTGGCTATG
AGGGAGAGCT GGCTGAATGA GAACCAGCGT CTGGTCTCCC AGGACAACTT TGGGTATGAG
CTGCCCGCAG TGGAGGCAGC CATGAAGAAA CACGAAGCGA TCGAGGCAGA CATTGCGGCC
TACGAGGAGC GGGTGCAGGG TGTGGCGGAG CTGGCCCAGG CATTGGCAGC CGAAGGCTAC
TACGATATCC GGCGGGTGGC AGCCCAGCGT GACAGCGTCC TGCGCCAGTG GGCCCTGCTA
ACTGGGCTTG TGGGTGCCCG GCGGACACGA CTTGAGCAGA ACCTTGCCCT GCAGAAGGTC
TTCCAGGAGA TGGTGTACAT GGTGGACTGG ATGGAGGAGA TGCAGGCTCA GCTGCTGTCC
CGGGAGTGTG GGCAGCACCT GGTGGAGGCA GACGACCTGT TGCAGAAGCA TGGACTGCTG
GAGGGAGACA TTGCCGCCCA GAGCGAGCGG GTGGAGGCTC TCAATGCCGC TGCCCTGCGC
TTCTCCCAGC TGCAGGGCTA CCAGCCCTGC GACCCGCAGG TCATCTGCAA CCGCGTGAAC
CACGTGCACG GCTGCCTGGC GGAGCTGCAG GAGCAGGCAG CGCGGCGACG CGCGGAGCTG
GAGGCTTCGC GGAGCCTGTG GGCGCTGCTG CAGGAGCTGG AGGAGGCCGA GAGCTGGGCG
CGCGACAAGG AGCGTCTCCT GGAGGCTGCG GGCGGCGGCG GTGCGGCGGG CGCAGCGGGC
GCAGCGGGAA CAGCGGGCGG CGCGCATGAC CTGTCCAGCA CAGCGCGCCT CCTGGCCCAG
CACAAGATCC TGCAGGGCGA GCTGGGCGGG CGGCGAGCGT TGCTGCAGCA GGCCCTGCGG
TGTGGCGAGG AGCTGGTTGC GGCCGGCGGT GCCGTCGGCC CGGGAGCAGA CACCGTGCAC
CTGGTAGGCC TGGCGGAGCG CGCGGCGAGC GCCCGGCGCC GCTGGCAGAG GCTGGAAGAG
GCGGCGGCGC GGCGAGAGCG GCGGCTGCAG GAGGCGCGGG CGCTGCACCA GTTCGGCGCT
GACCTCGACG GGCTGCTGGA CTGGCTTCGC GACGCTTACC GCCTGGCAGC CGCCGGTGAC
TTCGGCCACG ACGAAGCTTC CAGCCGCCGC CTGGCGCGCC AGCACCGCGC GCTCACCGGG
GAGGTGGAGG CACATCGCGG GCCCGTGAGC GGCCTGCGGC GCCAGCTGGC GACACTCGGG
GGTGCCAGTG GCGCAGGGCC ACTGGTGGTG GCGCTGCAGG TGCGCGTGGT GGAAGCAGAG
CAGTTGTTCG CTGAGGTGAC CGAAGTGGCG GCGCTGAGGC GCCAGTGGCT GCGGGACGCG
CTCGCTGTCT ACCGCATGTT TGGCGAGGTG CACGCGTGTG AGCTGTGGAT CGGCGAGAAG
GAGCAATGGC TGCTCTCCAT GCGTGTGCCG GATTCACTCG ACGACGTCGA GGTGGTGCAG
CACCGATTCG AGAGCCTGGA CCAAGAGATG AACAGCCTGA TGGGCCGCGT TCTGGACGTG
AACCACACAG TCCAGGAGCT GGTGGAAGGA GGCCACCCCA GTTCAGATGA GGTGCGTTCC
TGCCAGGACC ACCTCAACAG CAGGTGGAAC CGCATCGTGG AGCTAGTGGA ACAGCGCAAA
GAGGAAATGA GCGCGGTGCT GCTGGTGGAG AACCACGTGC TGGAGGTGGC CGAGGTGCGC
GCCCAGGTGC GTGAGAAGCG GAGAGCTGTG GAGAGCGCGC CCCGGGCCGG CGGCGCCCTG
CAGTGGCGTC TTAGCGGCCT AGAGGCCGCT CTGCAGGCGC TGGAGCCGCG CCAGGCGGCC
CTTCTGGAGG AGGCAGCCCT GCTGGCTGAG CGCTTCCCGG CGCAGGCGGC GCGGCTGCAC
CAGGGCGCGG AGGAGCTGGG CGCCGAGTGG GGCGCGCTAG CTAGCGCGGC TCAGGCCTGC
GGCGAGGCGG TGGCGGCAGC AGGGCGCCTG CAGCGCTTCC TACATGACCT CGACGCTTTC
CTGGACTGGC TCGTGCGCGC CCAGGAGGCG GCGGGCGGCA GCGAGGGGCC CCTGCCCAAC
AGCCTAGAAG AGGCGGACGC GCTGCTGGCG CGCCACGCTG CGCTCAAGGA GGAGGTGGAC
CAGCGCGAGG AAGACTATGC TCGCATCGTG GCGGCCAGCG AGGCGCTGCT GGCCGCCGAC
GGCGCAGAGC TGGGCCCGGG CCTGGCACTA GACGAGTGGC TGCCACACCT CGAACTTGGC
TGGCATAAAC TGCTCGGCTT GTGGGAGGCG CGCAGGGAGG CGCTGGTCCA GGCGCACATC
TACCAGCTCT TCCTGCGGGA TCTACGCCAG GCGCTCGTGG TGCTGCGTAA CCAGGAGATG
GCGCTGTCTG GTGCGGAGCT CCCGGGCACA GTGGAATCGG TGGAGGAGGC CTTGAAACAG
CACCGTGACT TTCTCACCAC CATGGAGCTG AGCCAGCAAA AGATGCAGGT GGCCGTGCAG
GCTGCAGAGG GCCTGCTGAG GCAGGGCAAC ATCTACGGGG AGCAGGCTCA GGAGGCTGTG
ACCCGGCTGC TGGAGAAGAA CCAAGAAAAC CAGTTACGGG CCCAGCAATG GATGCAAAAG
CTACATGACC AACTTGAGCT GCAGCACTTC CTCCGAGACT GCCACGAGCT GGATGGCTGG
ATCCATGAGA AGATGCTGAT GGCGCGGGAT GGCACGCGGG AGGACAACCA CAAGCTGCAT
AAGAGATGGC TCCGGCACCA GGCATTCATG GCCGAGCTGG CTCAGAATAA GGAGTGGCTG
GAGAAGATCG AGCGGGAGGG CCAGCAACTG ATGCAGGAGA AGCCCGAACT GGCGGCCTCC
GTGCGGAAGA AGCTGGGCGA GATCCGCCAG TGCTGGGCGG AGCTGGAGAG CACCACCCAG
GCCAAGGCAC GGCAGCTCTT TGAGGCCAGC AAAGCAGACC AGCTGGTGCA GAGCTTTGCT
GAGCTGGACA AGAAGCTCCT TCACATGGAG AGCCAGCTGC AAGACGTGGA CCCTGGAGGA
GACCTGGCCA CTGTCAACAG TCAGCTCAAG AAGCTGCAGT CCATGGAGTC GCAGGTGGAG
GAGTGGTACC GCGAGGTGGG AGAGCTGCAG GCGCAGACGG CGGCGCTGCC GCTGGAGCCG
GCGAGCAAGG AGCTGGTGGG TGAGCGGCAG AACGCGGTGG GCGAGCGCCT GGTGCGCCTG
CTCGAGCCGT TGCAGGAGCG CCGCCGCTTG CTGCTGGCTT CCAAGGAGTT GCACCAGGTG
GCGCACGACC TGGACGACGA GCTGGCATGG GTTCAGGAGC GGCTGCCACT GGCCATGCAG
ACAGAGCGAG GCAACGGTTT GCAGGCGGTC CAGCAGCACA TCAAAAAGAA CCAGGGCCTG
CGGCGGGAGA TCCAGGCGCA TGGGCCGCGC CTGGAGGAGG TGCTGGAGCG CGCGGGCGCG
CTGGCGTCGC TGCGCAGCCC GGAGGCAGAG GCAGTGCGCC GGGGCCTGGA GCAGCTGCAG
AGCGCCTGGG CCGGACTGCG GGAGGCTGCC GAGCGACGGC AGCAGGTGCT GGACGCCGCC
TTCCAGGTGG AGCAGTACTA CTTCGACGTG GCTGAGGTGG AGGCGTGGCT GGGCGAGCAG
GAGCTGCTCA TGATGAGTGA GGACAAGGGC AAGGACGAAC AGAGCACCCT GCAGCTGCTC
AAGAAACACC TGCAGCTGGA GCAAGGCGTG GAGAACTACG AGGAAAGCAT CGCGCAGCTG
TCGCGCCAGT GCCGGGCGCT GCTGGAGATG GGGCACCCGG ACAGCGAGCA GATCAGCCGG
CGGCAGTCTC AGGTGGACCG CCTGTACGTG GCGCTCAAGG AGCTGGGTGA GGAGCGCCGG
GTGGCTCTGG AACAGCAGTA CTGGCTGTAC CAGCTCAGCC GCCAGGTGAG CGAGCTTGAG
CACTGGATTG CCGAGAAGGA GGTGGTGGCT GGCTCACCCG AGCTCGGCCA GGACTTTGAG
CATGTCTCGG TGCTGCAGGA GAAATTCTCA GAGTTTGCCA GCGAGACAGG TATGGCAGGG
CGGGAACGGC TGGCAGCTGT GAACCAGATG GTGGATGAGC TGATCGAGTG TGGCCATACA
GCAGCGGCCA CCATGGCCGA GTGGAAGGAC GGACTGAACG AGGCCTGGGC TGAGCTGCTG
GAGCTCATGG GCACACGGGC CCAGCTGCTG GCCGCCTCTC GGGAGCTTCA TAAGTTCTTC
AGTGACGCCC GAGAGCTTCA GGGACAGATT GAGGAGAAGC GGAGGCGGCT GCCCCGCCTG
ACCACCCCGC CTGAGCCGAG ACCCAGTGCC AGTTCCATGC AGCGGACCCT GAGAGCCTTT
GAGCATGACC TGCAGCTCCT CGTGTCCCAG GTACGGCAGC TGCAGGAGGG GGCGGCCCAG
CTGCGGACGG TGTATGCGGG TGAACATGCC GAGGCCATCG CTAGCCGGGA GCAGGAGGTG
CTGCAGGGTT GGAAAGAGCT GCTGTCAGCC TGTGAGGATG CCCGCCTGCA TGTCAGCTCC
ACAGCCGACG CCCTGCGCTT CCACAGCCAA GTCCGCGACC TGCTCTCCTG GATGGATGGC
ATCGCCAGCC AGATTGGGGC AGCCGACAAG CCCAGGGACG TGTCATCAGT GGAGGTGCTC
ATGAACTACC ACCAGGGCCT GAAGACTGAG CTGGAGGCGC GGGTGCCTGA GCTGACCACC
TGCCAGGAGC TGGGGCGATC TCTGCTGCTC AACAAAAGTG CCATGGCTGA TGAGATCCAG
GCACAGCTGG ACAAGCTGGG AACCAGGAAG GAGGAGGTGT CGGAAAAGTG GGACCGCCAT
TGGGAGTGGC TGCAGCAGAT GCTGGAGGTG CACCAGTTTG CCCAGGAGGC GGTGGTGGCT
GATGCCTGGC TGACAGCCCA GGAGCCGCTC CTGCAGAGCC GGGAGCTGGG CAGCAGCGTG
GATGAGGTGG AGCAGCTTAT CCGGCGACAT GAGGCCTTCC GCAAAGCGGC TGCAGCCTGG
GAAGAGAGGT TCAGCTCTCT GCGGCGCCTG ACCACGATCG AGAAAATCAA AGCGGAACAG
AGCAAGCAGC CGCCTACCCC ACTGCTGGGG CGCAAGTTCT TTGGGGACCC CACGGAACTG
GCGGCCAAGG CGGCGCCCCT GCTGCGGCCA GGGGGCTATG AAAGGGGCTT GGAGCCCCTG
GCCCGCCGAG CCTCGGACAC GCTCTCGGCC GAGGTGCGGA CTCGGGTGGG GTATGTGCGC
CAGGAGCTCA AGCCCGAGCG CCTCCAGCCG CGCATTGACC GGCTGCCGGA GATCCCGGGG
AGGGTGGAGC CCGCGGCCCT GCCGGCCGCA CCAGAGGACG CGGCGGAGAC CCCCGCGACC
CCCGCGGCGG CGGAGCAGGT GCGGCCACGA CCGGAGCGCC AGGAGTCAGC TGATCGCGCG
GAGGAGCTGC CCAGGAGGCG GCGGCCTGAG CGGCAAGAGT CAGTCGATCA ATCCGAGGAG
GCTGCGCGGA GGCGGCGGCC GGAGCGGCAG GAGTCAGCGG AGCACGAGGC GGCACACAGC
CTTACCCTGG GCCGCTATGA GCAGATGGAG CGGCGGCGCG AGCGGCGTGA GCGGCGCTTG
GAGCGGCAGG AGTCCAGCGA ACAGGAGATG CCCATCAGAG GAGACCTGGT CAAGGGGAAG
GCCACCCTGG CTGACATTGT GGAACAGCTG CAGGAGAAAG AGGCAGGCCC AGGGCTGCCT
GCTGGGCCGT CGCTGCCTCA GCCACGCGAG CTTCCCCCAG GTCGCCTGCC CAACGGGCTT
GAGCTGCCCG AGCGGACACC TCGGCCGGAC CGGCCCCGGG CGCGGGACCG GCCCAAGCCG
CGACGGCGGC CGCGGCCCAG AGAGGGTGGT GAGGGCGGGG GAAGCCGGCG CTCGCGCTCC
GCCCCGGCCC AGGGCGGCTC CGCCCCCGCG CCTCCGCCAC CGCCCACTCA CACAGTGCAG
CACGAGGGCT TCCTACTGCG CAAGCGCGAG CTCGACGCTA ACCGCAAGTC GTCCAACCGG
TCGTGGGTGA GCCTGTACTG TGTGCTTAGT AAGGGGGAAC TGGGCTTCTA CAAGGACTCC
AAGGGCCCGG CATCCGGGAG CACACACGGT GGGGAACCGC TGCTCAGCCT GCACAAGGCC
ACCAGCGAGG TGGCTAGTGA CTACAAGAAA AAGAAGCATG TCTTCAAGCT CCAGACCCAG
GATGGCAGTG AGTTTTTGCT CCAGGCAAAA GATGAGGAGG AGATGAACGG CTGGCTGGAG
GCTGTAGCTT CCTCGGTGGC GGAACACGCA GAGATCGCCC GCTGGGGCCA GACACTACCC
ACTACTTCAT CCACAGATGA GGGCAACCCT AAGAGGGAAG GCGGAGATCG CAGGGCCAGC
GGGCGCAGGA AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu04416
Clone ID Related Accession (Same CDS sequence) NM_025213
Accession Version NM_025213.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2037bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product spectrin beta chain, non-erythrocytic 4 isoform sigma5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF311856.1 and DA514440.1. On Sep 30, 2006 this sequence version replaced gi:13435160. Transcript Variant: This variant (sigma5) contains a distinct 5' and 3' UTR and lacks an in-frame portion of the 5' and 3' coding region, compared to variant sigma1. The resulting isoform (sigma5), also known as the 77 kDa isoform, has distinct and shorter N- and C-termini when compared to isoform sigma1. ##Evidence-Data-START## Transcript exon combination :: AF311856.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGCTGATGG CGCGGGATGG CACGCGGGAG GACAACCACA AGCTGCATAA GAGATGGCTC 
CGGCACCAGG CATTCATGGC CGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATCGAG
CGGGAGGGCC AGCAACTGAT GCAGGAGAAG CCCGAACTGG CGGCCTCCGT GCGGAAGAAG
CTGGGCGAGA TCCGCCAGTG CTGGGCGGAG CTGGAGAGCA CCACCCAGGC CAAGGCACGG
CAGCTCTTTG AGGCCAGCAA AGCAGACCAG CTGGTGCAGA GCTTTGCTGA GCTGGACAAG
AAGCTCCTTC ACATGGAGAG CCAGCTGCAA GACGTGGACC CTGGAGGAGA CCTGGCCACT
GTCAACAGTC AGCTCAAGAA GCTGCAGTCC ATGGAGTCGC AGGTGGAGGA GTGGTACCGC
GAGGTGGGAG AGCTGCAGGC GCAGACGGCG GCGCTGCCGC TGGAGCCGGC GAGCAAGGAG
CTGGTGGGTG AGCGGCAGAA CGCGGTGGGC GAGCGCCTGG TGCGCCTGCT CGAGCCGTTG
CAGGAGCGCC GCCGCTTGCT GCTGGCTTCC AAGGAGTTGC ACCAGGTGGC GCACGACCTG
GACGACGAGC TGGCATGGGT TCAGGAGCGG CTGCCACTGG CCATGCAGAC AGAGCGAGGC
AACGGTTTGC AGGCGGTCCA GCAGCACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC
CAGGCGCATG GGCCGCGCCT GGAGGAGGTG CTGGAGCGCG CGGGCGCGCT GGCGTCGCTG
CGCAGCCCGG AGGCAGAGGC AGTGCGCCGG GGCCTGGAGC AGCTGCAGAG CGCCTGGGCC
GGACTGCGGG AGGCTGCCGA GCGACGGCAG CAGGTGCTGG ACGCCGCCTT CCAGGTGGAG
CAGTACTACT TCGACGTGGC TGAGGTGGAG GCGTGGCTGG GCGAGCAGGA GCTGCTCATG
ATGAGTGAGG ACAAGGGCAA GGACGAACAG AGCACCCTGC AGCTGCTCAA GAAACACCTG
CAGCTGGAGC AAGGCGTGGA GAACTACGAG GAAAGCATCG CGCAGCTGTC GCGCCAGTGC
CGGGCGCTGC TGGAGATGGG GCACCCGGAC AGCGAGCAGA TCAGCCGGCG GCAGTCTCAG
GTGGACCGCC TGTACGTGGC GCTCAAGGAG CTGGGTGAGG AGCGCCGGGT GGCTCTGGAA
CAGCAGTACT GGCTGTACCA GCTCAGCCGC CAGGTGAGCG AGCTTGAGCA CTGGATTGCC
GAGAAGGAGG TGGTGGCTGG CTCACCCGAG CTCGGCCAGG ACTTTGAGCA TGTCTCGGTG
CTGCAGGAGA AATTCTCAGA GTTTGCCAGC GAGACAGGTA TGGCAGGGCG GGAACGGCTG
GCAGCTGTGA ACCAGATGGT GGATGAGCTG ATCGAGTGTG GCCATACAGC AGCGGCCACC
ATGGCCGAGT GGAAGGACGG ACTGAACGAG GCCTGGGCTG AGCTGCTGGA GCTCATGGGC
ACACGGGCCC AGCTGCTGGC CGCCTCTCGG GAGCTTCATA AGTTCTTCAG TGACGCCCGA
GAGCTTCAGG GACAGATTGA GGAGAAGCGG AGGCGGCTGC CCCGCCTGAC CACCCCGCCT
GAGCCGAGAC CCAGTGCCAG TTCCATGCAG CGGACCCTGA GAGCCTTTGA GCATGACCTG
CAGCTCCTCG TGTCCCAGGT ACGGCAGCTG CAGGAGGGGG CGGCCCAGCT GCGGACGGTG
TATGCGGGTG AACATGCCGA GGCCATCGCT AGCCGGGAGC AGGAGGTGCT GCAGGGTTGG
AAAGAGCTGC TGTCAGCCTG TGAGGATGCC CGCCTGCATG TCAGCTCCAC AGCCGACGCC
CTGCGCTTCC ACAGCCAAGT CCGCGACCTG CTCTCCTGGA TGGATGGCAT CGCCAGCCAG
ATTGGGGCAG CCGACAAGCC CAGGTGCCCC TCATCCCTCC TCGGGCTTCC TGCCTCCCCC
TGGTGGCCTA CCCCAGCCAC CCCCAGCCCA TTGACAGCCC CCTTCTCAAT GGAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_079489.2
CDS128..2164
Misc Feature(1)131..697(+)
Misc Feature(2)380..397(+)
Misc Feature(3)701..1336(+)
Misc Feature(4)1013..1030(+)
Misc Feature(5)1019..1657(+)
Misc Feature(6)1331..1348(+)
Misc Feature(7)1658..>2095(+)
Misc Feature(8)1976..1993(+)
Exon (1)1..103
Gene:SPTBN4
Gene Synonym:
Exon (2)104..250
Gene:SPTBN4
Gene Synonym:
Exon (3)251..514
Gene:SPTBN4
Gene Synonym:
Exon (4)515..739
Gene:SPTBN4
Gene Synonym:
Exon (5)740..829
Gene:SPTBN4
Gene Synonym:
Exon (6)830..1108
Gene:SPTBN4
Gene Synonym:
Exon (7)1109..1239
Gene:SPTBN4
Gene Synonym:
Exon (8)1240..1444
Gene:SPTBN4
Gene Synonym:
Exon (9)1445..1825
Gene:SPTBN4
Gene Synonym:
Exon (10)1826..2419
Gene:SPTBN4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_025213
Organism Homo sapiens (human)
Definition Homo sapiens spectrin, beta, non-erythrocytic 4 (SPTBN4), transcript variant sigma5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_025213

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGCTGATGG CGCGGGATGG CACGCGGGAG GACAACCACA AGCTGCATAA GAGATGGCTC 
CGGCACCAGG CATTCATGGC CGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATCGAG
CGGGAGGGCC AGCAACTGAT GCAGGAGAAG CCCGAACTGG CGGCCTCCGT GCGGAAGAAG
CTGGGCGAGA TCCGCCAGTG CTGGGCGGAG CTGGAGAGCA CCACCCAGGC CAAGGCACGG
CAGCTCTTTG AGGCCAGCAA AGCAGACCAG CTGGTGCAGA GCTTTGCTGA GCTGGACAAG
AAGCTCCTTC ACATGGAGAG CCAGCTGCAA GACGTGGACC CTGGAGGAGA CCTGGCCACT
GTCAACAGTC AGCTCAAGAA GCTGCAGTCC ATGGAGTCGC AGGTGGAGGA GTGGTACCGC
GAGGTGGGAG AGCTGCAGGC GCAGACGGCG GCGCTGCCGC TGGAGCCGGC GAGCAAGGAG
CTGGTGGGTG AGCGGCAGAA CGCGGTGGGC GAGCGCCTGG TGCGCCTGCT CGAGCCGTTG
CAGGAGCGCC GCCGCTTGCT GCTGGCTTCC AAGGAGTTGC ACCAGGTGGC GCACGACCTG
GACGACGAGC TGGCATGGGT TCAGGAGCGG CTGCCACTGG CCATGCAGAC AGAGCGAGGC
AACGGTTTGC AGGCGGTCCA GCAGCACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC
CAGGCGCATG GGCCGCGCCT GGAGGAGGTG CTGGAGCGCG CGGGCGCGCT GGCGTCGCTG
CGCAGCCCGG AGGCAGAGGC AGTGCGCCGG GGCCTGGAGC AGCTGCAGAG CGCCTGGGCC
GGACTGCGGG AGGCTGCCGA GCGACGGCAG CAGGTGCTGG ACGCCGCCTT CCAGGTGGAG
CAGTACTACT TCGACGTGGC TGAGGTGGAG GCGTGGCTGG GCGAGCAGGA GCTGCTCATG
ATGAGTGAGG ACAAGGGCAA GGACGAACAG AGCACCCTGC AGCTGCTCAA GAAACACCTG
CAGCTGGAGC AAGGCGTGGA GAACTACGAG GAAAGCATCG CGCAGCTGTC GCGCCAGTGC
CGGGCGCTGC TGGAGATGGG GCACCCGGAC AGCGAGCAGA TCAGCCGGCG GCAGTCTCAG
GTGGACCGCC TGTACGTGGC GCTCAAGGAG CTGGGTGAGG AGCGCCGGGT GGCTCTGGAA
CAGCAGTACT GGCTGTACCA GCTCAGCCGC CAGGTGAGCG AGCTTGAGCA CTGGATTGCC
GAGAAGGAGG TGGTGGCTGG CTCACCCGAG CTCGGCCAGG ACTTTGAGCA TGTCTCGGTG
CTGCAGGAGA AATTCTCAGA GTTTGCCAGC GAGACAGGTA TGGCAGGGCG GGAACGGCTG
GCAGCTGTGA ACCAGATGGT GGATGAGCTG ATCGAGTGTG GCCATACAGC AGCGGCCACC
ATGGCCGAGT GGAAGGACGG ACTGAACGAG GCCTGGGCTG AGCTGCTGGA GCTCATGGGC
ACACGGGCCC AGCTGCTGGC CGCCTCTCGG GAGCTTCATA AGTTCTTCAG TGACGCCCGA
GAGCTTCAGG GACAGATTGA GGAGAAGCGG AGGCGGCTGC CCCGCCTGAC CACCCCGCCT
GAGCCGAGAC CCAGTGCCAG TTCCATGCAG CGGACCCTGA GAGCCTTTGA GCATGACCTG
CAGCTCCTCG TGTCCCAGGT ACGGCAGCTG CAGGAGGGGG CGGCCCAGCT GCGGACGGTG
TATGCGGGTG AACATGCCGA GGCCATCGCT AGCCGGGAGC AGGAGGTGCT GCAGGGTTGG
AAAGAGCTGC TGTCAGCCTG TGAGGATGCC CGCCTGCATG TCAGCTCCAC AGCCGACGCC
CTGCGCTTCC ACAGCCAAGT CCGCGACCTG CTCTCCTGGA TGGATGGCAT CGCCAGCCAG
ATTGGGGCAG CCGACAAGCC CAGGTGCCCC TCATCCCTCC TCGGGCTTCC TGCCTCCCCC
TGGTGGCCTA CCCCAGCCAC CCCCAGCCCA TTGACAGCCC CCTTCTCAAT GGAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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