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RAG2 recombination activating gene 2 [Homo sapiens (human)]

Gene Symbol RAG2
Entrez Gene ID 5897
Full Name recombination activating gene 2
Synonyms RAG-2
General protein information
Preferred Names
V(D)J recombination-activating protein 2
Names
V(D)J recombination-activating protein 2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p13

Summary This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

179616

Disorder Html: Severe combined immunodeficiency, B cell-negative, 601457 (3);





The following RAG2 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAG2 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu02396 NM_001243785 Homo sapiens recombination activating gene 2 (RAG2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu02396 NM_000536 Homo sapiens recombination activating gene 2 (RAG2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $379
OHu02396 NM_001243786 Homo sapiens recombination activating gene 2 (RAG2), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu02396
Accession Version NM_001243785.1
Sequence Information ORF Nucleotide Sequence (Length: 1584bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product V(D)J recombination-activating protein 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB146077.1, DB124002.1, AK292664.1, AW058148.1 and BC022397.1. Summary: This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (3) includes an alternate exon in the 5' UTR compared to variant 1. Variants 1, 3 and 4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DB124002.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145240, SAMEA2154529 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC
TTTCTTAGAA GGTTGTTTGA TTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001230714.1
CDS401..1984
Misc Feature(1)227..229(+)
Misc Feature(2)551..1567(+)
Misc Feature(3)1643..1843(+)
Misc Feature(4)1652..1828(+)
Misc Feature(5)1655..1843(+)
Exon (1)1..178
Gene:RAG2
Gene Synonym:
Exon (2)179..373
Gene:RAG2
Gene Synonym:
Exon (3)374..2626
Gene:RAG2
Gene Synonym:
Translation
Position Chain Variation Link
1 1 a, g dbSNP:185359195
42 42 a, g dbSNP:759799357
69 69 c, t dbSNP:73455545
74 74 a, g dbSNP:567981266
108 108 a, g dbSNP:547663028
114 114 g, t dbSNP:527948817
154 154 c, t dbSNP:536995581
196 196 c, t dbSNP:539949074
238 238 c, t dbSNP:756648542
264 264 c, t dbSNP:570874878
292 292 a, g dbSNP:745883953
385 385 c, t dbSNP:577304779
386 386 a, g dbSNP:766352307
399 399 c, t dbSNP:781432309
414 414 a, t dbSNP:143415103
421 421 a, c dbSNP:750499687
422 422 a, g dbSNP:150762709
432 432 a, g dbSNP:780060712
435 435 c, t dbSNP:146584017
439 439 a, c dbSNP:753764938
451 451 a, g dbSNP:569058130
456 456 g, t dbSNP:142852996
457 457 c, t dbSNP:752572568
459 459 c, t dbSNP:767174819
463 463 a, g dbSNP:142797325
468 468 a, g dbSNP:751073669
502 502 -, a dbSNP:746734641
504 504 c, g dbSNP:148508754
504 504 -, g dbSNP:777706845
515 515 a, g dbSNP:121917897
519 519 a, c dbSNP:762460908
523 523 c, g, t dbSNP:121917895
533 533 a, g dbSNP:770312622
535 535 c, t dbSNP:762130187
540 540 a, t dbSNP:776913146
541 541 g, t dbSNP:768914369
542 542 c, g dbSNP:767119176
543 543 c, t dbSNP:747263600
546 546 a, c dbSNP:370447243
553 553 a, g dbSNP:772223078
557 557 a, c dbSNP:774097244
561 561 a, g dbSNP:377416657
565 565 c, t dbSNP:777813230
574 574 a, g dbSNP:202020106
578 578 a, g dbSNP:752522715
581 581 a, g dbSNP:780915477
590 590 a, g dbSNP:535374501
613 613 c, t dbSNP:751114608
617 617 c, t dbSNP:193922574
618 618 a, g dbSNP:762407838
621 621 a, c dbSNP:749971416
630 630 a, c dbSNP:121918574
638 638 a, c, t dbSNP:762369105
640 640 c, g dbSNP:144703986
642 642 a, g dbSNP:777051349
644 644 a, g dbSNP:768839588
652 652 g, t dbSNP:761118163
653 653 c, g dbSNP:775526056
662 662 a, g dbSNP:772457166
666 666 a, g dbSNP:745913815
668 668 c, g dbSNP:149769148
678 678 c, t dbSNP:774630367
683 683 a, g dbSNP:36001797
696 696 c, g dbSNP:747968724
707 707 a, g dbSNP:781202485
713 713 g, t dbSNP:754677523
726 726 a, t dbSNP:201258007
727 727 c, t dbSNP:566382309
728 728 a, c, g dbSNP:193922575
734 734 a, g dbSNP:373703454
744 744 a, g dbSNP:764797359
745 745 -, c dbSNP:36041987
747 747 a, t dbSNP:756799907
751 751 c, t dbSNP:754244428
762 762 c, t dbSNP:764684990
767 767 c, t dbSNP:147319483
768 768 a, g dbSNP:144012817
769 769 c, t dbSNP:369277180
774 774 -, ca dbSNP:786205469
775 775 a, g dbSNP:759902640
776 776 c, g dbSNP:774716621
777 777 a, g dbSNP:775839683
792 792 c, g dbSNP:771076457
795 795 a, g dbSNP:375033313
815 815 a, g dbSNP:749407904
816 816 g, t dbSNP:776388094
817 817 g, t dbSNP:373022390
818 818 c, t dbSNP:147155709
837 837 a, g dbSNP:140046628
843 843 a, g dbSNP:779879427
850 850 a, c dbSNP:758044229
853 853 c, t dbSNP:745459546
861 861 c, t dbSNP:17852002
869 869 g, t dbSNP:756676209
875 875 a, c, t dbSNP:764485070
877 877 c, t dbSNP:141659100
879 879 c, t dbSNP:756694972
885 885 g, t dbSNP:753106164
886 886 a, g dbSNP:767924789
893 893 a, g dbSNP:759992403
906 906 a, c dbSNP:774665408
914 914 -, tg dbSNP:772196872
918 918 a, g dbSNP:766735610
928 928 c, t dbSNP:762907049
937 937 a, g dbSNP:189751142
947 947 c, t dbSNP:786205468
949 949 c, t dbSNP:768461432
950 950 c, t dbSNP:760735171
952 952 a, g dbSNP:775412618
970 970 a, g dbSNP:771800680
974 974 a, g dbSNP:372867141
986 986 a, t dbSNP:778497478
991 991 g, t dbSNP:770657068
995 995 g, t dbSNP:748727021
1020 1020 a, t dbSNP:777401257
1028 1028 a, g dbSNP:755582592
1038 1038 a, c dbSNP:753196074
1044 1044 a, c, t dbSNP:35691292
1066 1066 c, t dbSNP:752008728
1075 1075 c, g dbSNP:368808756
1082 1082 a, g dbSNP:763291155
1084 1084 c, t dbSNP:145031483
1085 1085 c, t dbSNP:765298019
1086 1086 a, g, t dbSNP:121917894
1087 1087 a, g dbSNP:772031809
1095 1095 a, g dbSNP:550672306
1097 1097 c, g dbSNP:774181734
1118 1118 a, c, t dbSNP:374781438
1127 1127 a, g dbSNP:777301719
1141 1141 a, g dbSNP:34092949
1149 1149 -, ca dbSNP:779092895
1150 1150 -, a dbSNP:748017653
1150 1150 a, g dbSNP:747690737
1152 1152 c, g, t dbSNP:755587413
1156 1156 a, g dbSNP:751957269
1164 1164 a, g dbSNP:780531526
1176 1176 c, t dbSNP:758697173
1180 1180 g, t dbSNP:750603413
1183 1183 a, t dbSNP:765404128
1191 1191 c, t dbSNP:761845264
1203 1203 a, g dbSNP:368935791
1204 1204 g, t dbSNP:767420787
1209 1209 a, g dbSNP:149241274
1214 1214 a, g, t dbSNP:117899975
1220 1220 c, g dbSNP:762677522
1230 1230 a, g dbSNP:772880437
1234 1234 c, g dbSNP:769370947
1254 1254 g, t dbSNP:121917896
1256 1256 a, t dbSNP:542395331
1259 1259 -, t dbSNP:754975137
1263 1263 -, a dbSNP:755941834
1264 1264 c, t dbSNP:776334417
1266 1266 a, t dbSNP:769279401
1272 1272 a, c, t dbSNP:112927992
1278 1278 a, g dbSNP:16929093
1284 1284 a, g dbSNP:770231022
1294 1294 a, t dbSNP:144855259
1298 1298 c, t dbSNP:746253611
1299 1299 a, g dbSNP:138857320
1308 1308 a, g dbSNP:757524729
1309 1309 g, t dbSNP:141025671
1313 1313 c, g dbSNP:370666759
1314 1314 c, t dbSNP:756192655
1315 1315 a, g dbSNP:751463938
1341 1341 g, t dbSNP:766257880
1342 1342 a, c dbSNP:762777615
1348 1348 a, g dbSNP:545910812
1363 1363 c, t dbSNP:764967374
1365 1365 c, t dbSNP:17856658
1368 1368 a, g dbSNP:139419162
1371 1371 a, g dbSNP:761622056
1372 1372 c, t dbSNP:776425229
1377 1377 a, c, t dbSNP:781129987
1379 1379 a, g dbSNP:776028357
1390 1390 c, t dbSNP:772499379
1395 1395 c, g dbSNP:746221994
1396 1396 a, g dbSNP:771084047
1411 1411 -, a dbSNP:780078535
1425 1425 a, g dbSNP:367615315
1429 1429 c, g, t dbSNP:747177680
1430 1430 c, t dbSNP:777991833
1435 1435 c, t dbSNP:35942246
1437 1437 a, g dbSNP:752759155
1441 1441 a, g dbSNP:766318035
1464 1464 c, t dbSNP:758321058
1474 1474 a, g dbSNP:557047531
1476 1476 a, g dbSNP:765055291
1478 1478 a, g dbSNP:761711845
1483 1483 a, g dbSNP:371025129
1490 1490 -, aaca dbSNP:756470858
1492 1492 c, g dbSNP:150349031
1495 1495 c, t dbSNP:140519815
1517 1517 a, g dbSNP:774916204
1520 1520 c, g dbSNP:772618891
1524 1524 a, c dbSNP:760029599
1527 1527 a, c, g dbSNP:192958391
1530 1530 c, t dbSNP:749683351
1531 1531 c, t dbSNP:777940606
1538 1538 g, t dbSNP:151269922
1542 1542 -, ct dbSNP:750849852
1547 1547 a, g dbSNP:377569152
1551 1551 g, t dbSNP:781192001
1554 1554 -, gttt dbSNP:767854656
1557 1557 g, t dbSNP:755086501
1558 1558 a, c dbSNP:34629171
1573 1573 c, t dbSNP:750267658
1574 1574 a, g dbSNP:145860209
1584 1584 c, g dbSNP:757081652
1587 1587 a, g dbSNP:753730066
1589 1589 a, g dbSNP:763820748
1590 1590 a, g dbSNP:760303499
1591 1591 -, tga dbSNP:567942993
1591 1591 c, t dbSNP:752248630
1597 1597 g, t dbSNP:200473984
1598 1598 c, g dbSNP:140682926
1602 1602 c, g dbSNP:774798451
1605 1605 a, g dbSNP:199798089
1618 1618 a, t dbSNP:749005936
1623 1623 a, c dbSNP:763281636
1634 1634 c, g dbSNP:773624427
1647 1647 g, t dbSNP:193922572
1651 1651 c, t dbSNP:748248673
1653 1653 c, t dbSNP:781487639
1668 1668 a, g dbSNP:768567592
1675 1675 a, g dbSNP:747119015
1676 1676 a, g dbSNP:778966787
1682 1682 a, c dbSNP:757316422
1695 1695 c, g dbSNP:753677011
1696 1696 a, g dbSNP:777778248
1707 1707 a, c dbSNP:201928772
1709 1709 a, g dbSNP:193922573
1713 1713 a, t dbSNP:752234153
1716 1716 a, g dbSNP:767223651
1717 1717 a, c dbSNP:201044101
1720 1720 a, c dbSNP:754413772
1722 1722 a, c dbSNP:372228472
1723 1723 c, t dbSNP:370624669
1724 1724 a, g dbSNP:763513886
1729 1729 a, g dbSNP:773710101
1747 1747 a, g dbSNP:376226076
1752 1752 c, g dbSNP:121918575
1764 1764 a, g dbSNP:776679669
1765 1765 c, t dbSNP:369276846
1768 1768 g, t dbSNP:768965648
1772 1772 g, t dbSNP:747067538
1773 1773 g, t dbSNP:775773909
1785 1785 c, g dbSNP:770988511
1790 1790 c, t dbSNP:189020262
1791 1791 a, c, g, t dbSNP:147748696
1792 1792 c, t dbSNP:780684558
1801 1801 c, t dbSNP:754682981
1803 1803 -, atct dbSNP:786205616
1803 1803 a, g dbSNP:751064709
1804 1804 c, t dbSNP:528679382
1821 1821 a, g dbSNP:757913323
1831 1831 c, t dbSNP:750878183
1833 1833 a, g dbSNP:121918573
1841 1841 c, g dbSNP:762054841
1843 1843 c, t dbSNP:560115611
1844 1844 a, g dbSNP:764210172
1859 1859 a, g dbSNP:374892361
1860 1860 c, g dbSNP:761045499
1869 1869 a, c dbSNP:775863600
1872 1872 c, t dbSNP:772225125
1877 1877 a, g dbSNP:745969837
1878 1878 a, g dbSNP:2037101
1883 1883 c, t dbSNP:772985232
1892 1892 a, c, t dbSNP:373151027
1901 1901 a, c dbSNP:781104028
1904 1904 a, g dbSNP:145614809
1915 1915 c, g dbSNP:577434324
1916 1916 c, t dbSNP:140089249
1917 1917 a, g dbSNP:144812762
1924 1924 -, a dbSNP:751978214
1926 1926 a, g, t dbSNP:779267024
1931 1931 a, g dbSNP:757841294
1942 1942 c, g dbSNP:754150018
1965 1965 g, t dbSNP:764457023
1979 1979 g, t dbSNP:149401912
1984 1984 a, g dbSNP:761137785
1988 1988 -, t dbSNP:764833518
1991 1991 a, g dbSNP:752922147
1995 1995 a, g dbSNP:376130960
2002 2002 a, c dbSNP:759664706
2014 2014 a, g dbSNP:774545556
2025 2025 g, t dbSNP:769610598
2030 2030 a, t dbSNP:374011646
2032 2032 a, t dbSNP:776446942
2036 2036 a, t dbSNP:546979744
2043 2043 a, g dbSNP:372838463
2120 2120 a, c dbSNP:763674526
2128 2128 g, t dbSNP:12280515
2134 2134 a, g dbSNP:574639787
2167 2167 a, c dbSNP:12280564
2172 2172 c, t dbSNP:142073874
2185 2185 c, t dbSNP:770212281
2211 2211 c, t dbSNP:113343318
2214 2214 a, g dbSNP:552856146
2270 2270 g, t dbSNP:539728776
2312 2312 a, g dbSNP:10836573
2361 2361 a, g dbSNP:3740956
2419 2419 a, g dbSNP:143457071
2488 2488 c, g, t dbSNP:186462541
2498 2498 c, g dbSNP:367981268
2528 2528 c, t dbSNP:536630555
2566 2566 c, t dbSNP:568192751
2586 2586 c, t dbSNP:559845453
2612 2612 a, g dbSNP:563665491

Target ORF information:

RefSeq Version NM_001243785
Organism Homo sapiens (human)
Definition Homo sapiens recombination activating gene 2 (RAG2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001243785

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC
TTTCTTAGAA GGTTGTTTGA TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02396
Accession Version NM_000536.3
Sequence Information ORF Nucleotide Sequence (Length: 1584bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product V(D)J recombination-activating protein 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB146077.1, AK292664.1, AW058148.1 and BC022397.1. On May 14, 2010 this sequence version replaced gi:151301079. Summary: This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1) represents the shortest transcript. Variants 1, 3 and 4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC022397.1, AK292664.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC
TTTCTTAGAA GGTTGTTTGA TTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000527.2
CDS206..1789
Misc Feature(1)113..115(+)
Misc Feature(2)356..1372(+)
Misc Feature(3)1448..1648(+)
Misc Feature(4)1457..1633(+)
Misc Feature(5)1460..1648(+)
Exon (1)1..178
Gene:RAG2
Gene Synonym:
Exon (2)179..2431
Gene:RAG2
Gene Synonym:
Translation
Position Chain Variation Link
1 1 a, g dbSNP:185359195
42 42 a, g dbSNP:759799357
69 69 c, t dbSNP:73455545
74 74 a, g dbSNP:567981266
108 108 a, g dbSNP:547663028
114 114 g, t dbSNP:527948817
154 154 c, t dbSNP:536995581
190 190 c, t dbSNP:577304779
191 191 a, g dbSNP:766352307
204 204 c, t dbSNP:781432309
219 219 a, t dbSNP:143415103
226 226 a, c dbSNP:750499687
227 227 a, g dbSNP:150762709
237 237 a, g dbSNP:780060712
240 240 c, t dbSNP:146584017
244 244 a, c dbSNP:753764938
256 256 a, g dbSNP:569058130
261 261 g, t dbSNP:142852996
262 262 c, t dbSNP:752572568
264 264 c, t dbSNP:767174819
268 268 a, g dbSNP:142797325
273 273 a, g dbSNP:751073669
307 307 -, a dbSNP:746734641
309 309 c, g dbSNP:148508754
309 309 -, g dbSNP:777706845
320 320 a, g dbSNP:121917897
324 324 a, c dbSNP:762460908
328 328 c, g, t dbSNP:121917895
338 338 a, g dbSNP:770312622
340 340 c, t dbSNP:762130187
345 345 a, t dbSNP:776913146
346 346 g, t dbSNP:768914369
347 347 c, g dbSNP:767119176
348 348 c, t dbSNP:747263600
351 351 a, c dbSNP:370447243
358 358 a, g dbSNP:772223078
362 362 a, c dbSNP:774097244
366 366 a, g dbSNP:377416657
370 370 c, t dbSNP:777813230
379 379 a, g dbSNP:202020106
383 383 a, g dbSNP:752522715
386 386 a, g dbSNP:780915477
395 395 a, g dbSNP:535374501
418 418 c, t dbSNP:751114608
422 422 c, t dbSNP:193922574
423 423 a, g dbSNP:762407838
426 426 a, c dbSNP:749971416
435 435 a, c dbSNP:121918574
443 443 a, c, t dbSNP:762369105
445 445 c, g dbSNP:144703986
447 447 a, g dbSNP:777051349
449 449 a, g dbSNP:768839588
457 457 g, t dbSNP:761118163
458 458 c, g dbSNP:775526056
467 467 a, g dbSNP:772457166
471 471 a, g dbSNP:745913815
473 473 c, g dbSNP:149769148
483 483 c, t dbSNP:774630367
488 488 a, g dbSNP:36001797
501 501 c, g dbSNP:747968724
512 512 a, g dbSNP:781202485
518 518 g, t dbSNP:754677523
531 531 a, t dbSNP:201258007
532 532 c, t dbSNP:566382309
533 533 a, c, g dbSNP:193922575
539 539 a, g dbSNP:373703454
549 549 a, g dbSNP:764797359
550 550 -, c dbSNP:36041987
552 552 a, t dbSNP:756799907
556 556 c, t dbSNP:754244428
567 567 c, t dbSNP:764684990
572 572 c, t dbSNP:147319483
573 573 a, g dbSNP:144012817
574 574 c, t dbSNP:369277180
579 579 -, ca dbSNP:786205469
580 580 a, g dbSNP:759902640
581 581 c, g dbSNP:774716621
582 582 a, g dbSNP:775839683
597 597 c, g dbSNP:771076457
600 600 a, g dbSNP:375033313
620 620 a, g dbSNP:749407904
621 621 g, t dbSNP:776388094
622 622 g, t dbSNP:373022390
623 623 c, t dbSNP:147155709
642 642 a, g dbSNP:140046628
648 648 a, g dbSNP:779879427
655 655 a, c dbSNP:758044229
658 658 c, t dbSNP:745459546
666 666 c, t dbSNP:17852002
674 674 g, t dbSNP:756676209
680 680 a, c, t dbSNP:764485070
682 682 c, t dbSNP:141659100
684 684 c, t dbSNP:756694972
690 690 g, t dbSNP:753106164
691 691 a, g dbSNP:767924789
698 698 a, g dbSNP:759992403
711 711 a, c dbSNP:774665408
719 719 -, tg dbSNP:772196872
723 723 a, g dbSNP:766735610
733 733 c, t dbSNP:762907049
742 742 a, g dbSNP:189751142
752 752 c, t dbSNP:786205468
754 754 c, t dbSNP:768461432
755 755 c, t dbSNP:760735171
757 757 a, g dbSNP:775412618
775 775 a, g dbSNP:771800680
779 779 a, g dbSNP:372867141
791 791 a, t dbSNP:778497478
796 796 g, t dbSNP:770657068
800 800 g, t dbSNP:748727021
825 825 a, t dbSNP:777401257
833 833 a, g dbSNP:755582592
843 843 a, c dbSNP:753196074
849 849 a, c, t dbSNP:35691292
871 871 c, t dbSNP:752008728
880 880 c, g dbSNP:368808756
887 887 a, g dbSNP:763291155
889 889 c, t dbSNP:145031483
890 890 c, t dbSNP:765298019
891 891 a, g, t dbSNP:121917894
892 892 a, g dbSNP:772031809
900 900 a, g dbSNP:550672306
902 902 c, g dbSNP:774181734
923 923 a, c, t dbSNP:374781438
932 932 a, g dbSNP:777301719
946 946 a, g dbSNP:34092949
954 954 -, ca dbSNP:779092895
955 955 -, a dbSNP:748017653
955 955 a, g dbSNP:747690737
957 957 c, g, t dbSNP:755587413
961 961 a, g dbSNP:751957269
969 969 a, g dbSNP:780531526
981 981 c, t dbSNP:758697173
985 985 g, t dbSNP:750603413
988 988 a, t dbSNP:765404128
996 996 c, t dbSNP:761845264
1008 1008 a, g dbSNP:368935791
1009 1009 g, t dbSNP:767420787
1014 1014 a, g dbSNP:149241274
1019 1019 a, g, t dbSNP:117899975
1025 1025 c, g dbSNP:762677522
1035 1035 a, g dbSNP:772880437
1039 1039 c, g dbSNP:769370947
1059 1059 g, t dbSNP:121917896
1061 1061 a, t dbSNP:542395331
1064 1064 -, t dbSNP:754975137
1068 1068 -, a dbSNP:755941834
1069 1069 c, t dbSNP:776334417
1071 1071 a, t dbSNP:769279401
1077 1077 a, c, t dbSNP:112927992
1083 1083 a, g dbSNP:16929093
1089 1089 a, g dbSNP:770231022
1099 1099 a, t dbSNP:144855259
1103 1103 c, t dbSNP:746253611
1104 1104 a, g dbSNP:138857320
1113 1113 a, g dbSNP:757524729
1114 1114 g, t dbSNP:141025671
1118 1118 c, g dbSNP:370666759
1119 1119 c, t dbSNP:756192655
1120 1120 a, g dbSNP:751463938
1146 1146 g, t dbSNP:766257880
1147 1147 a, c dbSNP:762777615
1153 1153 a, g dbSNP:545910812
1168 1168 c, t dbSNP:764967374
1170 1170 c, t dbSNP:17856658
1173 1173 a, g dbSNP:139419162
1176 1176 a, g dbSNP:761622056
1177 1177 c, t dbSNP:776425229
1182 1182 a, c, t dbSNP:781129987
1184 1184 a, g dbSNP:776028357
1195 1195 c, t dbSNP:772499379
1200 1200 c, g dbSNP:746221994
1201 1201 a, g dbSNP:771084047
1216 1216 -, a dbSNP:780078535
1230 1230 a, g dbSNP:367615315
1234 1234 c, g, t dbSNP:747177680
1235 1235 c, t dbSNP:777991833
1240 1240 c, t dbSNP:35942246
1242 1242 a, g dbSNP:752759155
1246 1246 a, g dbSNP:766318035
1269 1269 c, t dbSNP:758321058
1279 1279 a, g dbSNP:557047531
1281 1281 a, g dbSNP:765055291
1283 1283 a, g dbSNP:761711845
1288 1288 a, g dbSNP:371025129
1295 1295 -, aaca dbSNP:756470858
1297 1297 c, g dbSNP:150349031
1300 1300 c, t dbSNP:140519815
1322 1322 a, g dbSNP:774916204
1325 1325 c, g dbSNP:772618891
1329 1329 a, c dbSNP:760029599
1332 1332 a, c, g dbSNP:192958391
1335 1335 c, t dbSNP:749683351
1336 1336 c, t dbSNP:777940606
1343 1343 g, t dbSNP:151269922
1347 1347 -, ct dbSNP:750849852
1352 1352 a, g dbSNP:377569152
1356 1356 g, t dbSNP:781192001
1359 1359 -, gttt dbSNP:767854656
1362 1362 g, t dbSNP:755086501
1363 1363 a, c dbSNP:34629171
1378 1378 c, t dbSNP:750267658
1379 1379 a, g dbSNP:145860209
1389 1389 c, g dbSNP:757081652
1392 1392 a, g dbSNP:753730066
1394 1394 a, g dbSNP:763820748
1395 1395 a, g dbSNP:760303499
1396 1396 -, tga dbSNP:567942993
1396 1396 c, t dbSNP:752248630
1402 1402 g, t dbSNP:200473984
1403 1403 c, g dbSNP:140682926
1407 1407 c, g dbSNP:774798451
1410 1410 a, g dbSNP:199798089
1423 1423 a, t dbSNP:749005936
1428 1428 a, c dbSNP:763281636
1439 1439 c, g dbSNP:773624427
1452 1452 g, t dbSNP:193922572
1456 1456 c, t dbSNP:748248673
1458 1458 c, t dbSNP:781487639
1473 1473 a, g dbSNP:768567592
1480 1480 a, g dbSNP:747119015
1481 1481 a, g dbSNP:778966787
1487 1487 a, c dbSNP:757316422
1500 1500 c, g dbSNP:753677011
1501 1501 a, g dbSNP:777778248
1512 1512 a, c dbSNP:201928772
1514 1514 a, g dbSNP:193922573
1518 1518 a, t dbSNP:752234153
1521 1521 a, g dbSNP:767223651
1522 1522 a, c dbSNP:201044101
1525 1525 a, c dbSNP:754413772
1527 1527 a, c dbSNP:372228472
1528 1528 c, t dbSNP:370624669
1529 1529 a, g dbSNP:763513886
1534 1534 a, g dbSNP:773710101
1552 1552 a, g dbSNP:376226076
1557 1557 c, g dbSNP:121918575
1569 1569 a, g dbSNP:776679669
1570 1570 c, t dbSNP:369276846
1573 1573 g, t dbSNP:768965648
1577 1577 g, t dbSNP:747067538
1578 1578 g, t dbSNP:775773909
1590 1590 c, g dbSNP:770988511
1595 1595 c, t dbSNP:189020262
1596 1596 a, c, g, t dbSNP:147748696
1597 1597 c, t dbSNP:780684558
1606 1606 c, t dbSNP:754682981
1608 1608 -, atct dbSNP:786205616
1608 1608 a, g dbSNP:751064709
1609 1609 c, t dbSNP:528679382
1626 1626 a, g dbSNP:757913323
1636 1636 c, t dbSNP:750878183
1638 1638 a, g dbSNP:121918573
1646 1646 c, g dbSNP:762054841
1648 1648 c, t dbSNP:560115611
1649 1649 a, g dbSNP:764210172
1664 1664 a, g dbSNP:374892361
1665 1665 c, g dbSNP:761045499
1674 1674 a, c dbSNP:775863600
1677 1677 c, t dbSNP:772225125
1682 1682 a, g dbSNP:745969837
1683 1683 a, g dbSNP:2037101
1688 1688 c, t dbSNP:772985232
1697 1697 a, c, t dbSNP:373151027
1706 1706 a, c dbSNP:781104028
1709 1709 a, g dbSNP:145614809
1720 1720 c, g dbSNP:577434324
1721 1721 c, t dbSNP:140089249
1722 1722 a, g dbSNP:144812762
1729 1729 -, a dbSNP:751978214
1731 1731 a, g, t dbSNP:779267024
1736 1736 a, g dbSNP:757841294
1747 1747 c, g dbSNP:754150018
1770 1770 g, t dbSNP:764457023
1784 1784 g, t dbSNP:149401912
1789 1789 a, g dbSNP:761137785
1793 1793 -, t dbSNP:764833518
1796 1796 a, g dbSNP:752922147
1800 1800 a, g dbSNP:376130960
1807 1807 a, c dbSNP:759664706
1819 1819 a, g dbSNP:774545556
1830 1830 g, t dbSNP:769610598
1835 1835 a, t dbSNP:374011646
1837 1837 a, t dbSNP:776446942
1841 1841 a, t dbSNP:546979744
1848 1848 a, g dbSNP:372838463
1925 1925 a, c dbSNP:763674526
1933 1933 g, t dbSNP:12280515
1939 1939 a, g dbSNP:574639787
1972 1972 a, c dbSNP:12280564
1977 1977 c, t dbSNP:142073874
1990 1990 c, t dbSNP:770212281
2016 2016 c, t dbSNP:113343318
2019 2019 a, g dbSNP:552856146
2075 2075 g, t dbSNP:539728776
2117 2117 a, g dbSNP:10836573
2166 2166 a, g dbSNP:3740956
2224 2224 a, g dbSNP:143457071
2293 2293 c, g, t dbSNP:186462541
2303 2303 c, g dbSNP:367981268
2333 2333 c, t dbSNP:536630555
2371 2371 c, t dbSNP:568192751
2391 2391 c, t dbSNP:559845453
2417 2417 a, g dbSNP:563665491

Target ORF information:

RefSeq Version NM_000536
Organism Homo sapiens (human)
Definition Homo sapiens recombination activating gene 2 (RAG2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000536

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC
TTTCTTAGAA GGTTGTTTGA TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02396
Accession Version NM_001243786.1
Sequence Information ORF Nucleotide Sequence (Length: 1584bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product V(D)J recombination-activating protein 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB146077.1, DB143931.1, AC139427.3, AK292664.1, AW058148.1 and BC022397.1. Summary: This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (4) includes an alternate exon in the 5' UTR compared to variant 1. Variants 1, 3 and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DB143931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145240, SAMEA2159931 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC
TTTCTTAGAA GGTTGTTTGA TTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001230715.1
CDS595..2178
Misc Feature(1)421..423(+)
Misc Feature(2)745..1761(+)
Misc Feature(3)1837..2037(+)
Misc Feature(4)1846..2022(+)
Misc Feature(5)1849..2037(+)
Exon (1)1..178
Gene:RAG2
Gene Synonym:
Exon (2)179..567
Gene:RAG2
Gene Synonym:
Exon (3)568..2820
Gene:RAG2
Gene Synonym:
Translation
Position Chain Variation Link
1 1 a, g dbSNP:185359195
42 42 a, g dbSNP:759799357
69 69 c, t dbSNP:73455545
74 74 a, g dbSNP:567981266
108 108 a, g dbSNP:547663028
114 114 g, t dbSNP:527948817
154 154 c, t dbSNP:536995581
197 197 a, t dbSNP:545736905
247 247 a, g dbSNP:566893568
270 270 g, t dbSNP:181970754
308 308 a, g dbSNP:572068870
357 357 c, t dbSNP:553798853
390 390 c, t dbSNP:539949074
432 432 c, t dbSNP:756648542
458 458 c, t dbSNP:570874878
486 486 a, g dbSNP:745883953
579 579 c, t dbSNP:577304779
580 580 a, g dbSNP:766352307
593 593 c, t dbSNP:781432309
608 608 a, t dbSNP:143415103
615 615 a, c dbSNP:750499687
616 616 a, g dbSNP:150762709
626 626 a, g dbSNP:780060712
629 629 c, t dbSNP:146584017
633 633 a, c dbSNP:753764938
645 645 a, g dbSNP:569058130
650 650 g, t dbSNP:142852996
651 651 c, t dbSNP:752572568
653 653 c, t dbSNP:767174819
657 657 a, g dbSNP:142797325
662 662 a, g dbSNP:751073669
696 696 -, a dbSNP:746734641
698 698 c, g dbSNP:148508754
698 698 -, g dbSNP:777706845
709 709 a, g dbSNP:121917897
713 713 a, c dbSNP:762460908
717 717 c, g, t dbSNP:121917895
727 727 a, g dbSNP:770312622
729 729 c, t dbSNP:762130187
734 734 a, t dbSNP:776913146
735 735 g, t dbSNP:768914369
736 736 c, g dbSNP:767119176
737 737 c, t dbSNP:747263600
740 740 a, c dbSNP:370447243
747 747 a, g dbSNP:772223078
751 751 a, c dbSNP:774097244
755 755 a, g dbSNP:377416657
759 759 c, t dbSNP:777813230
768 768 a, g dbSNP:202020106
772 772 a, g dbSNP:752522715
775 775 a, g dbSNP:780915477
784 784 a, g dbSNP:535374501
807 807 c, t dbSNP:751114608
811 811 c, t dbSNP:193922574
812 812 a, g dbSNP:762407838
815 815 a, c dbSNP:749971416
824 824 a, c dbSNP:121918574
832 832 a, c, t dbSNP:762369105
834 834 c, g dbSNP:144703986
836 836 a, g dbSNP:777051349
838 838 a, g dbSNP:768839588
846 846 g, t dbSNP:761118163
847 847 c, g dbSNP:775526056
856 856 a, g dbSNP:772457166
860 860 a, g dbSNP:745913815
862 862 c, g dbSNP:149769148
872 872 c, t dbSNP:774630367
877 877 a, g dbSNP:36001797
890 890 c, g dbSNP:747968724
901 901 a, g dbSNP:781202485
907 907 g, t dbSNP:754677523
920 920 a, t dbSNP:201258007
921 921 c, t dbSNP:566382309
922 922 a, c, g dbSNP:193922575
928 928 a, g dbSNP:373703454
938 938 a, g dbSNP:764797359
939 939 -, c dbSNP:36041987
941 941 a, t dbSNP:756799907
945 945 c, t dbSNP:754244428
956 956 c, t dbSNP:764684990
961 961 c, t dbSNP:147319483
962 962 a, g dbSNP:144012817
963 963 c, t dbSNP:369277180
968 968 -, ca dbSNP:786205469
969 969 a, g dbSNP:759902640
970 970 c, g dbSNP:774716621
971 971 a, g dbSNP:775839683
986 986 c, g dbSNP:771076457
989 989 a, g dbSNP:375033313
1009 1009 a, g dbSNP:749407904
1010 1010 g, t dbSNP:776388094
1011 1011 g, t dbSNP:373022390
1012 1012 c, t dbSNP:147155709
1031 1031 a, g dbSNP:140046628
1037 1037 a, g dbSNP:779879427
1044 1044 a, c dbSNP:758044229
1047 1047 c, t dbSNP:745459546
1055 1055 c, t dbSNP:17852002
1063 1063 g, t dbSNP:756676209
1069 1069 a, c, t dbSNP:764485070
1071 1071 c, t dbSNP:141659100
1073 1073 c, t dbSNP:756694972
1079 1079 g, t dbSNP:753106164
1080 1080 a, g dbSNP:767924789
1087 1087 a, g dbSNP:759992403
1100 1100 a, c dbSNP:774665408
1108 1108 -, tg dbSNP:772196872
1112 1112 a, g dbSNP:766735610
1122 1122 c, t dbSNP:762907049
1131 1131 a, g dbSNP:189751142
1141 1141 c, t dbSNP:786205468
1143 1143 c, t dbSNP:768461432
1144 1144 c, t dbSNP:760735171
1146 1146 a, g dbSNP:775412618
1164 1164 a, g dbSNP:771800680
1168 1168 a, g dbSNP:372867141
1180 1180 a, t dbSNP:778497478
1185 1185 g, t dbSNP:770657068
1189 1189 g, t dbSNP:748727021
1214 1214 a, t dbSNP:777401257
1222 1222 a, g dbSNP:755582592
1232 1232 a, c dbSNP:753196074
1238 1238 a, c, t dbSNP:35691292
1260 1260 c, t dbSNP:752008728
1269 1269 c, g dbSNP:368808756
1276 1276 a, g dbSNP:763291155
1278 1278 c, t dbSNP:145031483
1279 1279 c, t dbSNP:765298019
1280 1280 a, g, t dbSNP:121917894
1281 1281 a, g dbSNP:772031809
1289 1289 a, g dbSNP:550672306
1291 1291 c, g dbSNP:774181734
1312 1312 a, c, t dbSNP:374781438
1321 1321 a, g dbSNP:777301719
1335 1335 a, g dbSNP:34092949
1343 1343 -, ca dbSNP:779092895
1344 1344 -, a dbSNP:748017653
1344 1344 a, g dbSNP:747690737
1346 1346 c, g, t dbSNP:755587413
1350 1350 a, g dbSNP:751957269
1358 1358 a, g dbSNP:780531526
1370 1370 c, t dbSNP:758697173
1374 1374 g, t dbSNP:750603413
1377 1377 a, t dbSNP:765404128
1385 1385 c, t dbSNP:761845264
1397 1397 a, g dbSNP:368935791
1398 1398 g, t dbSNP:767420787
1403 1403 a, g dbSNP:149241274
1408 1408 a, g, t dbSNP:117899975
1414 1414 c, g dbSNP:762677522
1424 1424 a, g dbSNP:772880437
1428 1428 c, g dbSNP:769370947
1448 1448 g, t dbSNP:121917896
1450 1450 a, t dbSNP:542395331
1453 1453 -, t dbSNP:754975137
1457 1457 -, a dbSNP:755941834
1458 1458 c, t dbSNP:776334417
1460 1460 a, t dbSNP:769279401
1466 1466 a, c, t dbSNP:112927992
1472 1472 a, g dbSNP:16929093
1478 1478 a, g dbSNP:770231022
1488 1488 a, t dbSNP:144855259
1492 1492 c, t dbSNP:746253611
1493 1493 a, g dbSNP:138857320
1502 1502 a, g dbSNP:757524729
1503 1503 g, t dbSNP:141025671
1507 1507 c, g dbSNP:370666759
1508 1508 c, t dbSNP:756192655
1509 1509 a, g dbSNP:751463938
1535 1535 g, t dbSNP:766257880
1536 1536 a, c dbSNP:762777615
1542 1542 a, g dbSNP:545910812
1557 1557 c, t dbSNP:764967374
1559 1559 c, t dbSNP:17856658
1562 1562 a, g dbSNP:139419162
1565 1565 a, g dbSNP:761622056
1566 1566 c, t dbSNP:776425229
1571 1571 a, c, t dbSNP:781129987
1573 1573 a, g dbSNP:776028357
1584 1584 c, t dbSNP:772499379
1589 1589 c, g dbSNP:746221994
1590 1590 a, g dbSNP:771084047
1605 1605 -, a dbSNP:780078535
1619 1619 a, g dbSNP:367615315
1623 1623 c, g, t dbSNP:747177680
1624 1624 c, t dbSNP:777991833
1629 1629 c, t dbSNP:35942246
1631 1631 a, g dbSNP:752759155
1635 1635 a, g dbSNP:766318035
1658 1658 c, t dbSNP:758321058
1668 1668 a, g dbSNP:557047531
1670 1670 a, g dbSNP:765055291
1672 1672 a, g dbSNP:761711845
1677 1677 a, g dbSNP:371025129
1684 1684 -, aaca dbSNP:756470858
1686 1686 c, g dbSNP:150349031
1689 1689 c, t dbSNP:140519815
1711 1711 a, g dbSNP:774916204
1714 1714 c, g dbSNP:772618891
1718 1718 a, c dbSNP:760029599
1721 1721 a, c, g dbSNP:192958391
1724 1724 c, t dbSNP:749683351
1725 1725 c, t dbSNP:777940606
1732 1732 g, t dbSNP:151269922
1736 1736 -, ct dbSNP:750849852
1741 1741 a, g dbSNP:377569152
1745 1745 g, t dbSNP:781192001
1748 1748 -, gttt dbSNP:767854656
1751 1751 g, t dbSNP:755086501
1752 1752 a, c dbSNP:34629171
1767 1767 c, t dbSNP:750267658
1768 1768 a, g dbSNP:145860209
1778 1778 c, g dbSNP:757081652
1781 1781 a, g dbSNP:753730066
1783 1783 a, g dbSNP:763820748
1784 1784 a, g dbSNP:760303499
1785 1785 -, tga dbSNP:567942993
1785 1785 c, t dbSNP:752248630
1791 1791 g, t dbSNP:200473984
1792 1792 c, g dbSNP:140682926
1796 1796 c, g dbSNP:774798451
1799 1799 a, g dbSNP:199798089
1812 1812 a, t dbSNP:749005936
1817 1817 a, c dbSNP:763281636
1828 1828 c, g dbSNP:773624427
1841 1841 g, t dbSNP:193922572
1845 1845 c, t dbSNP:748248673
1847 1847 c, t dbSNP:781487639
1862 1862 a, g dbSNP:768567592
1869 1869 a, g dbSNP:747119015
1870 1870 a, g dbSNP:778966787
1876 1876 a, c dbSNP:757316422
1889 1889 c, g dbSNP:753677011
1890 1890 a, g dbSNP:777778248
1901 1901 a, c dbSNP:201928772
1903 1903 a, g dbSNP:193922573
1907 1907 a, t dbSNP:752234153
1910 1910 a, g dbSNP:767223651
1911 1911 a, c dbSNP:201044101
1914 1914 a, c dbSNP:754413772
1916 1916 a, c dbSNP:372228472
1917 1917 c, t dbSNP:370624669
1918 1918 a, g dbSNP:763513886
1923 1923 a, g dbSNP:773710101
1941 1941 a, g dbSNP:376226076
1946 1946 c, g dbSNP:121918575
1958 1958 a, g dbSNP:776679669
1959 1959 c, t dbSNP:369276846
1962 1962 g, t dbSNP:768965648
1966 1966 g, t dbSNP:747067538
1967 1967 g, t dbSNP:775773909
1979 1979 c, g dbSNP:770988511
1984 1984 c, t dbSNP:189020262
1985 1985 a, c, g, t dbSNP:147748696
1986 1986 c, t dbSNP:780684558
1995 1995 c, t dbSNP:754682981
1997 1997 -, atct dbSNP:786205616
1997 1997 a, g dbSNP:751064709
1998 1998 c, t dbSNP:528679382
2015 2015 a, g dbSNP:757913323
2025 2025 c, t dbSNP:750878183
2027 2027 a, g dbSNP:121918573
2035 2035 c, g dbSNP:762054841
2037 2037 c, t dbSNP:560115611
2038 2038 a, g dbSNP:764210172
2053 2053 a, g dbSNP:374892361
2054 2054 c, g dbSNP:761045499
2063 2063 a, c dbSNP:775863600
2066 2066 c, t dbSNP:772225125
2071 2071 a, g dbSNP:745969837
2072 2072 a, g dbSNP:2037101
2077 2077 c, t dbSNP:772985232
2086 2086 a, c, t dbSNP:373151027
2095 2095 a, c dbSNP:781104028
2098 2098 a, g dbSNP:145614809
2109 2109 c, g dbSNP:577434324
2110 2110 c, t dbSNP:140089249
2111 2111 a, g dbSNP:144812762
2118 2118 -, a dbSNP:751978214
2120 2120 a, g, t dbSNP:779267024
2125 2125 a, g dbSNP:757841294
2136 2136 c, g dbSNP:754150018
2159 2159 g, t dbSNP:764457023
2173 2173 g, t dbSNP:149401912
2178 2178 a, g dbSNP:761137785
2182 2182 -, t dbSNP:764833518
2185 2185 a, g dbSNP:752922147
2189 2189 a, g dbSNP:376130960
2196 2196 a, c dbSNP:759664706
2208 2208 a, g dbSNP:774545556
2219 2219 g, t dbSNP:769610598
2224 2224 a, t dbSNP:374011646
2226 2226 a, t dbSNP:776446942
2230 2230 a, t dbSNP:546979744
2237 2237 a, g dbSNP:372838463
2314 2314 a, c dbSNP:763674526
2322 2322 g, t dbSNP:12280515
2328 2328 a, g dbSNP:574639787
2361 2361 a, c dbSNP:12280564
2366 2366 c, t dbSNP:142073874
2379 2379 c, t dbSNP:770212281
2405 2405 c, t dbSNP:113343318
2408 2408 a, g dbSNP:552856146
2464 2464 g, t dbSNP:539728776
2506 2506 a, g dbSNP:10836573
2555 2555 a, g dbSNP:3740956
2613 2613 a, g dbSNP:143457071
2682 2682 c, g, t dbSNP:186462541
2692 2692 c, g dbSNP:367981268
2722 2722 c, t dbSNP:536630555
2760 2760 c, t dbSNP:568192751
2780 2780 c, t dbSNP:559845453
2806 2806 a, g dbSNP:563665491

Target ORF information:

RefSeq Version NM_001243786
Organism Homo sapiens (human)
Definition Homo sapiens recombination activating gene 2 (RAG2), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001243786

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC
TTTCTTAGAA GGTTGTTTGA TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.