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RASA1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol RASA1
Entrez Gene ID 5921
Full Name RAS p21 protein activator (GTPase activating protein) 1
Synonyms CM-AVM, CMAVM, GAP, PKWS, RASA, RASGAP, p120GAP, p120RASGAP
General protein information
Preferred Names
ras GTPase-activating protein 1
Names
ras GTPase-activating protein 1
triphosphatase-activating protein
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

5

5q13.3

Summary The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues. [provided by RefSeq, May 2012]. lac of sum
Disorder MIM:

139150

Disorder Html: Parkes Weber slndrome, 608355 (3); Capillary

mRNA and Protein(s)

mRNA Protein Name
XM_011543525 XP_011541827 ras GTPase-activating protein 1 isoform X1
XM_011543526 XP_011541828 ras GTPase-activating protein 1 isoform X2
XM_011543527 XP_011541829 ras GTPase-activating protein 1 isoform X3
NM_002890 NP_002881 ras GTPase-activating protein 1 isoform 1
NM_022650 NP_072179 ras GTPase-activating protein 1 isoform 2



Homo sapiens (human) RASA1 NP_002881.1
Pan troglodytes (chimpanzee) RASA1 XP_517663.3
Macaca mulatta (Rhesus monkey) RASA1 XP_001084074.2
Canis lupus familiaris (dog) RASA1 XP_536302.3
Bos taurus (cattle) RASA1 NP_776874.1
Mus musculus (house mouse) Rasa1 NP_663427.2
Rattus norvegicus (Norway rat) Rasa1 NP_037267.1
Gallus gallus (chicken) RASA1 XP_424907.4
Danio rerio (zebrafish) rasa1a XP_001342009.2
Drosophila melanogaster (fruit fly) vap NP_727926.2
Xenopus (Silurana) tropicalis (western clawed frog) LOC100135092 NP_001107303.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RASA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu60197 XM_011543525 PREDICTED: Homo sapiens RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu60198 XM_011543526 PREDICTED: Homo sapiens RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu60199 XM_011543527 PREDICTED: Homo sapiens RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu18040
NM_002890 Homo sapiens RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $49.50
$99.00
OHu16849 NM_022650 Homo sapiens RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $615.30
$879.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu60197
Clone ID Related Accession (Same CDS sequence) XM_011543525
Accession Version XM_011543525.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3057bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ras GTPase-activating protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_034772.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGATGGCGG CCGAGGCCGG CAGTGAGGAG GGCGGCCCGG TAACAGCCGG AGCTGGAGGA 
GGCGGCGCGG CAGCGGGCTC CAGTGCCTAT CCCGCAGTGT GTCGGGTGAA GATACCCGCG
GCCCTGCCTG TGGCAGCCGC CCCCTATCCT GGGCTGGTGG AGACCGGAGT GGCTGGAACT
CTGGGTGGCG GAGCCGCTTT GGGGTCAGAG TTCCTAGGAG CCGGGTCTGT GGCAGGGGCA
CTGGGGGGAG CTGGACTGAC AGGGGGAGGT ACTGCTGCTG GCGTAGCTGG TGCTGCTGCT
GGCGTGGCCG GTGCTGCTGT TGCTGGACCT AGTGGAGACA TGGCTCTCAC CAAACTGCCC
ACTTCGTTGC TTGCTGAGAC TCTCGGGCCA GGCGGCGGTT TTCCCCCTCT GCCCCCTCCC
CCTTACCTGC CCCCTTTGGG GGCGGGCCTC GGGACAGTGG ACGAAGGTGA CTCTCTGGAT
GGACCAGAAT ACGAGGAGGA AGAGGTGGCC ATACCGTTGA CCGCTCCTCC AACTAACCAG
TGGTATCACG GAAAACTTGA CAGAACGATA GCAGAAGAAC GCCTCAGGCA GGCAGGGAAG
TCTGGCAGTT ATCTTATAAG AGAGAGTGAT CGGAGGCCAG GGTCCTTTGT ACTTTCATTT
CTTAGCCAGA TGAATGTTGT CAACCATTTT AGGATTATTG CTATGTGTGG AGATTACTAC
ATTGGTGGAA GACGTTTTTC TTCACTGTCA GACCTAATAG GTTATTACAG TCATGTTTCT
TGTTTGCTTA AAGGAGAAAA ATTACTTTAC CCAGTTGCAC CACCAGAGCC AGTAGAAGAT
AGAAGGCGTG TACGAGCTAT TCTACCTTAC ACAAAAGTAC CAGACACTGA TGAAATAAGT
TTCTTAAAAG GAGATATGTT CATTGTTCAT AATGAATTAG AAGATGGATG GATGTGGGTT
ACAAATTTAA GAACAGATGA ACAAGGCCTT ATTGTTGAAG ACCTAGTAGA AGAGGTGGGC
CGGGAAGAAG ATCCACATGA AGGAAAAATA TGGTTCCATG GGAAGATTTC CAAACAGGAA
GCTTATAATT TACTAATGAC AGTTGGTCAA GTCTGCAGTT TTCTTGTGAG GCCCTCAGAT
AATACTCCTG GCGATTATTC ACTTTATTTC CGGACCAATG AAAATATTCA GCGATTTAAA
ATATGTCCAA CGCCAAACAA TCAGTTTATG ATGGGAGGCC GGTATTATAA CAGCATTGGG
GACATCATAG ATCACTATCG AAAAGAACAG ATTGTTGAAG GATATTATCT TAAGGAACCT
GTACCAATGC AGGATCAAGA ACAAGTACTC AATGACACAG TGGATGGCAA GGAAATCTAT
AATACCATCC GTCGTAAAAC AAAGGATGCC TTTTATAAAA ACATTGTTAA GAAAGGTTAT
CTTCTGAAAA AGGGCAAAGG AAAACGTTGG AAAAATTTAT ATTTTATCTT AGAGGGTAGT
GATGCCCAAC TTATTTATTT TGAAAGCGAA AAACGAGCTA CCAAACCAAA AGGATTAATA
GATCTCAGTG TATGTTCTGT CTATGTCGTT CATGATAGTC TCTTTGGCAG GCCAAACTGT
TTTCAGATAG TAGTTCAGCA CTTTAGTGAA GAACATTACA TCTTTTACTT TGCAGGAGAA
ACTCCAGAAC AAGCAGAGGA TTGGATGAAA GGTCTGCAGG CATTTTGCAA TTTACGGAAA
AGTAGTCCAG GGACATCCAA TAAACGCCTT CGTCAGGTCA GCAGCCTTGT TTTACATATT
GAAGAAGCCC ATAAACTCCC AGTAAAACAT TTTACTAATC CATATTGTAA CATCTACCTG
AATAGTGTCC AAGTAGCAAA AACTCATGCA AGGGAAGGGC AAAACCCAGT ATGGTCAGAA
GAGTTTGTCT TTGATGATCT TCCTCCTGAC ATCAATAGAT TTGAAATAAC TCTTAGTAAT
AAAACAAAGA AAAGCAAAGA TCCTGATATC TTATTTATGC GCTGCCAGTT GAGCCGATTA
CAGAAAGGGC ATGCCACAGA TGAATGGTTT CTGCTCAGCT CCCATATACC ATTAAAAGGT
ATTGAACCAG GGTCCCTGCG TGTTCGAGCA CGATACTCTA TGGAAAAAAT CATGCCAGAA
GAAGAGTACA GTGAATTTAA AGAGCTTATA CTGCAAAAGG AACTTCATGT AGTCTATGCT
TTATCACATG TATGTGGACA AGACCGAACA CTACTGGCCA GCATCCTACT GAGGATTTTT
CTTCACGAAA AGCTTGAATC GTTGTTGTTA TGCACACTAA ATGACAGAGA AATAAGCATG
GAAGATGAAG CCACTACCCT ATTTCGAGCC ACAACACTTG CAAGCACCTT GATGGAGCAG
TATATGAAAG CCACTGCTAC ACAGTTTGTT CATCATGCTT TGAAAGACTC TATTTTAAAG
ATAATGGAAA GCAAGCAGTC TTGTGAGTTA AGTCCATCAA AGTTAGAAAA AAATGAAGAT
GTGAACACTA ATTTAACACA CCTATTGAAC ATACTTTCAG AGCTTGTGGA GAAAATATTC
ATGGCTTCAG AAATACTTCC ACCTGGTTTT GTTTTTCTTC GACTCATCTG TCCTGCCATC
CTGAATCCAC GGATGTTCAA TATCATCTCA GATTCTCCAT CTCCTATTGC TGCAAGAACA
CTGATATTAG TGGCTAAATC TGTGCAGAAC TTAGCAAATC TTGTGGAATT TGGAGCTAAG
GAGCCCTACA TGGAAGGTGT CAATCCATTC ATCAAAAGCA ACAAACATCG TATGATCATG
TTTTTAGATG AACTTGGGAA TGTACCTGAA CTTCCGGACA CTACAGAGCA TTCTAGAACG
GACCTGTCCC GTGATTTAGC AGCATTGCAT GAGATTTGCG TGGCTCATTC AGATGAACTT
CGAACGCTCA GTAATGAGCG TGGTGCACAG CAGCACGTAT TGAAAAAGCT TCTGGCTATA
ACAGAACTGC TTCAACAAAA ACAAAACCAG TATACAAAAA CCAATGATGT CAGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011541827.1
CDS652..3708
Misc Feature(1)1132..1443(+)
Misc Feature(2)1213..1344(+)
Misc Feature(3)1339..1419(+)
Misc Feature(4)1492..1668(+)
Misc Feature(5)1699..1929(+)
Misc Feature(6)1723..1851(+)
Misc Feature(7)1846..1929(+)
Misc Feature(8)2116..2379(+)
Misc Feature(9)2422..2799(+)
Misc Feature(10)2743..3696(+)
Misc Feature(11)2791..3699(+)
Misc Feature(12)2896..3414(+)
Translation

Target ORF information:

RefSeq Version XM_011543525
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543525

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGATGGCGG CCGAGGCCGG CAGTGAGGAG GGCGGCCCGG TAACAGCCGG AGCTGGAGGA 
GGCGGCGCGG CAGCGGGCTC CAGTGCCTAT CCCGCAGTGT GTCGGGTGAA GATACCCGCG
GCCCTGCCTG TGGCAGCCGC CCCCTATCCT GGGCTGGTGG AGACCGGAGT GGCTGGAACT
CTGGGTGGCG GAGCCGCTTT GGGGTCAGAG TTCCTAGGAG CCGGGTCTGT GGCAGGGGCA
CTGGGGGGAG CTGGACTGAC AGGGGGAGGT ACTGCTGCTG GCGTAGCTGG TGCTGCTGCT
GGCGTGGCCG GTGCTGCTGT TGCTGGACCT AGTGGAGACA TGGCTCTCAC CAAACTGCCC
ACTTCGTTGC TTGCTGAGAC TCTCGGGCCA GGCGGCGGTT TTCCCCCTCT GCCCCCTCCC
CCTTACCTGC CCCCTTTGGG GGCGGGCCTC GGGACAGTGG ACGAAGGTGA CTCTCTGGAT
GGACCAGAAT ACGAGGAGGA AGAGGTGGCC ATACCGTTGA CCGCTCCTCC AACTAACCAG
TGGTATCACG GAAAACTTGA CAGAACGATA GCAGAAGAAC GCCTCAGGCA GGCAGGGAAG
TCTGGCAGTT ATCTTATAAG AGAGAGTGAT CGGAGGCCAG GGTCCTTTGT ACTTTCATTT
CTTAGCCAGA TGAATGTTGT CAACCATTTT AGGATTATTG CTATGTGTGG AGATTACTAC
ATTGGTGGAA GACGTTTTTC TTCACTGTCA GACCTAATAG GTTATTACAG TCATGTTTCT
TGTTTGCTTA AAGGAGAAAA ATTACTTTAC CCAGTTGCAC CACCAGAGCC AGTAGAAGAT
AGAAGGCGTG TACGAGCTAT TCTACCTTAC ACAAAAGTAC CAGACACTGA TGAAATAAGT
TTCTTAAAAG GAGATATGTT CATTGTTCAT AATGAATTAG AAGATGGATG GATGTGGGTT
ACAAATTTAA GAACAGATGA ACAAGGCCTT ATTGTTGAAG ACCTAGTAGA AGAGGTGGGC
CGGGAAGAAG ATCCACATGA AGGAAAAATA TGGTTCCATG GGAAGATTTC CAAACAGGAA
GCTTATAATT TACTAATGAC AGTTGGTCAA GTCTGCAGTT TTCTTGTGAG GCCCTCAGAT
AATACTCCTG GCGATTATTC ACTTTATTTC CGGACCAATG AAAATATTCA GCGATTTAAA
ATATGTCCAA CGCCAAACAA TCAGTTTATG ATGGGAGGCC GGTATTATAA CAGCATTGGG
GACATCATAG ATCACTATCG AAAAGAACAG ATTGTTGAAG GATATTATCT TAAGGAACCT
GTACCAATGC AGGATCAAGA ACAAGTACTC AATGACACAG TGGATGGCAA GGAAATCTAT
AATACCATCC GTCGTAAAAC AAAGGATGCC TTTTATAAAA ACATTGTTAA GAAAGGTTAT
CTTCTGAAAA AGGGCAAAGG AAAACGTTGG AAAAATTTAT ATTTTATCTT AGAGGGTAGT
GATGCCCAAC TTATTTATTT TGAAAGCGAA AAACGAGCTA CCAAACCAAA AGGATTAATA
GATCTCAGTG TATGTTCTGT CTATGTCGTT CATGATAGTC TCTTTGGCAG GCCAAACTGT
TTTCAGATAG TAGTTCAGCA CTTTAGTGAA GAACATTACA TCTTTTACTT TGCAGGAGAA
ACTCCAGAAC AAGCAGAGGA TTGGATGAAA GGTCTGCAGG CATTTTGCAA TTTACGGAAA
AGTAGTCCAG GGACATCCAA TAAACGCCTT CGTCAGGTCA GCAGCCTTGT TTTACATATT
GAAGAAGCCC ATAAACTCCC AGTAAAACAT TTTACTAATC CATATTGTAA CATCTACCTG
AATAGTGTCC AAGTAGCAAA AACTCATGCA AGGGAAGGGC AAAACCCAGT ATGGTCAGAA
GAGTTTGTCT TTGATGATCT TCCTCCTGAC ATCAATAGAT TTGAAATAAC TCTTAGTAAT
AAAACAAAGA AAAGCAAAGA TCCTGATATC TTATTTATGC GCTGCCAGTT GAGCCGATTA
CAGAAAGGGC ATGCCACAGA TGAATGGTTT CTGCTCAGCT CCCATATACC ATTAAAAGGT
ATTGAACCAG GGTCCCTGCG TGTTCGAGCA CGATACTCTA TGGAAAAAAT CATGCCAGAA
GAAGAGTACA GTGAATTTAA AGAGCTTATA CTGCAAAAGG AACTTCATGT AGTCTATGCT
TTATCACATG TATGTGGACA AGACCGAACA CTACTGGCCA GCATCCTACT GAGGATTTTT
CTTCACGAAA AGCTTGAATC GTTGTTGTTA TGCACACTAA ATGACAGAGA AATAAGCATG
GAAGATGAAG CCACTACCCT ATTTCGAGCC ACAACACTTG CAAGCACCTT GATGGAGCAG
TATATGAAAG CCACTGCTAC ACAGTTTGTT CATCATGCTT TGAAAGACTC TATTTTAAAG
ATAATGGAAA GCAAGCAGTC TTGTGAGTTA AGTCCATCAA AGTTAGAAAA AAATGAAGAT
GTGAACACTA ATTTAACACA CCTATTGAAC ATACTTTCAG AGCTTGTGGA GAAAATATTC
ATGGCTTCAG AAATACTTCC ACCTGGTTTT GTTTTTCTTC GACTCATCTG TCCTGCCATC
CTGAATCCAC GGATGTTCAA TATCATCTCA GATTCTCCAT CTCCTATTGC TGCAAGAACA
CTGATATTAG TGGCTAAATC TGTGCAGAAC TTAGCAAATC TTGTGGAATT TGGAGCTAAG
GAGCCCTACA TGGAAGGTGT CAATCCATTC ATCAAAAGCA ACAAACATCG TATGATCATG
TTTTTAGATG AACTTGGGAA TGTACCTGAA CTTCCGGACA CTACAGAGCA TTCTAGAACG
GACCTGTCCC GTGATTTAGC AGCATTGCAT GAGATTTGCG TGGCTCATTC AGATGAACTT
CGAACGCTCA GTAATGAGCG TGGTGCACAG CAGCACGTAT TGAAAAAGCT TCTGGCTATA
ACAGAACTGC TTCAACAAAA ACAAAACCAG TATACAAAAA CCAATGATGT CAGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60198
Clone ID Related Accession (Same CDS sequence) XM_011543526
Accession Version XM_011543526.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3000bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ras GTPase-activating protein 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_034772.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGATGGCGG CCGAGGCCGG CAGTGAGGAG GGCGGCCCGG TAACAGCCGG AGCTGGAGGA 
GGCGGCGCGG CAGCGGGCTC CAGTGCCTAT CCCGCAGTGT GTCGGGTGAA GATACCCGCG
GCCCTGCCTG TGGCAGCCGC CCCCTATCCT GGGCTGGTGG AGACCGGAGT GGCTGGAACT
CTGGGTGGCG GAGCCGCTTT GGGGTCAGAG TTCCTAGGAG CCGGGTCTGT GGCAGGGGCA
CTGGGGGGAG CTGGACTGAC AGGGGGAGGT ACTGCTGCTG GCGTAGCTGG TGCTGCTGCT
GGCGTGGCCG GTGCTGCTGT TGCTGGACCT AGTGGAGACA TGGCTCTCAC CAAACTGCCC
ACTTCGTTGC TTGCTGAGAC TCTCGGGCCA GGCGGCGGTT TTCCCCCTCT GCCCCCTCCC
CCTTACCTGC CCCCTTTGGG GGCGGGCCTC GGGACAGTGG ACGAAGGTGA CTCTCTGGAT
GGACCAGAAT ACGAGGAGGA AGAGGTGGCC ATACCGTTGA CCGCTCCTCC AACTAACCAG
TGGTATCACG GAAAACTTGA CAGAACGATA GCAGAAGAAC GCCTCAGGCA GGCAGGGAAG
TCTGGCAGTT ATCTTATAAG AGAGAGTGAT CGGAGGCCAG GGTCCTTTGT ACTTTCATTT
CTTAGCCAGA TGAATGTTGT CAACCATTTT AGGATTATTG CTATGTGTGG AGATTACTAC
ATTGGTGGAA GACGTTTTTC TTCACTGTCA GACCTAATAG GTTATTACAG TCATGTTTCT
TGTTTGCTTA AAGGAGAAAA ATTACTTTAC CCAGTTGCAC CACCAGAGCC AGTAGAAGAT
AGAAGGCGTG TACGAGCTAT TCTACCTTAC ACAAAAGTAC CAGACACTGA TGAAATAAGT
TTCTTAAAAG GAGATATGTT CATTGTTCAT AATGAATTAG AAGATGGATG GATGTGGGTT
ACAAATTTAA GAACAGATGA ACAAGGCCTT ATTGTTGAAG ACCTAGTAGA AGAGGTGGGC
CGGGAAGAAG ATCCACATGA AGGAAAAATA TGGTTCCATG GGAAGATTTC CAAACAGGAA
GCTTATAATT TACTAATGAC AGTTGGTCAA GTCTGCAGTT TTCTTGTGAG GCCCTCAGAT
AATACTCCTG GCGATTATTC ACTTTATTTC CGGACCAATG AAAATATTCA GCGATTTAAA
ATATGTCCAA CGCCAAACAA TCAGTTTATG ATGGGAGGCC GGTATTATAA CAGCATTGGG
GACATCATAG ATCACTATCG AAAAGAACAG ATTGTTGAAG GATATTATCT TAAGGAACCT
GTACCAATGC AGGATCAAGA ACAAGTACTC AATGACACAG TGGATGGCAA GGAAATCTAT
AATACCATCC GTCGTAAAAC AAAGGATGCC TTTTATAAAA ACATTGTTAA GAAAGGTTAT
CTTCTGAAAA AGGGCAAAGG AAAACGTTGG AAAAATTTAT ATTTTATCTT AGAGGGTAGT
GATGCCCAAC TTATTTATTT TGAAAGCGAA AAACGAGCTA CCAAACCAAA AGGATTAATA
GATCTCAGTG TATGTTCTGT CTATGTCGTT CATGATAGTC TCTTTGGCAG GCCAAACTGT
TTTCAGATAG TAGTTCAGCA CTTTAGTGAA GAACATTACA TCTTTTACTT TGCAGGAGAA
ACTCCAGAAC AAGCAGAGGA TTGGATGAAA GGTCTGCAGG CATTTTGCAA TTTACGGAAA
AGTAGTCCAG GGACATCCAA TAAACGCCTT CGTCAGGTCA GCAGCCTTGT TTTACATATT
GAAGAAGCCC ATAAACTCCC AGTAAAACAT TTTACTAATC CATATTGTAA CATCTACCTG
AATAGTGTCC AAGTAGCAAA AACTCATGCA AGGGAAGGGC AAAACCCAGT ATGGTCAGAA
GAGTTTGTCT TTGATGATCT TCCTCCTGAC ATCAATAGAT TTGAAATAAC TCTTAGTAAT
AAAACAAAGA AAAGCAAAGA TCCTGATATC TTATTTATGC GCTGCCAGTT GAGCCGATTA
CAGAAAGGGC ATGCCACAGA TGAATGGTTT CTGCTCAGCT CCCATATACC ATTAAAAGGT
ATTGAACCAG GGTCCCTGCG TGTTCGAGCA CGATACTCTA TGGAAAAAAT CATGCCAGAA
GAAGAGTACA GTGAATTTAA AGAGCTTATA CTGCAAAAGG AACTTCATGT AGTCTATGCT
TTATCACATG TATGTGGACA AGACCGAACA CTACTGGCCA GCATCCTACT GAGGATTTTT
CTTCACGAAA AGCTTGAATC GTTGTTGTTA TGCACACTAA ATGACAGAGA AATAAGCATG
GAAGATGAAG CCACTACCCT ATTTCGAGCC ACAACACTTG CAAGCACCTT GATGGAGCAG
TATATGAAAG CCACTGCTAC ACAGTTTGTT CATCATGCTT TGAAAGACTC TATTTTAAAG
ATAATGGAAA GCAAGCAGTC TTGTGAGTTA AGTCCATCAA AGTTAGAAAA AAATGAAGAT
GTGAACACTA ATTTAACACA CCTATTGAAC ATACTTTCAG AGCTTGTGGA GAAAATATTC
ATGGCTTCAG AAATACTTCC ACCGACATTG AGATATATTT ATGGGTGTTT ACAGAAATCT
GTTCAGCATA AGTGGCCTAC AAATACCACC ATGAGAACAA GAGTTGTTAG TGGTTTTGTT
TTTCTTCGAC TCATCTGTCC TGCCATCCTG AATCCACGGA TGTTCAATAT CATCTCAGAT
TCTCCATCTC CTATTGCTGC AAGAACACTG ATATTAGTGG CTAAATCTGT GCAGAACTTA
GCAAATCTTG TGGAATTTGG AGCTAAGGAG CCCTACATGG AAGGTGTCAA TCCATTCATC
AAAAGCAACA AACATCGTAT GATCATGTTT TTAGATGAAC TTGGGCTTTT TTACTTTGCT
GCTACTATGT GGGGCGTGAA GCAAGACGAT GACACAGTTC TGACCAGGTT AAATAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011541828.1
CDS652..3651
Misc Feature(1)1132..1443(+)
Misc Feature(2)1213..1344(+)
Misc Feature(3)1339..1419(+)
Misc Feature(4)1492..1668(+)
Misc Feature(5)1699..1929(+)
Misc Feature(6)1723..1851(+)
Misc Feature(7)1846..1929(+)
Misc Feature(8)2116..2379(+)
Misc Feature(9)2422..2799(+)
Misc Feature(10)2743..3573(+)
Misc Feature(11)2791..>3576(+)
Misc Feature(12)2896..3501(+)
Translation

Target ORF information:

RefSeq Version XM_011543526
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543526

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGATGGCGG CCGAGGCCGG CAGTGAGGAG GGCGGCCCGG TAACAGCCGG AGCTGGAGGA 
GGCGGCGCGG CAGCGGGCTC CAGTGCCTAT CCCGCAGTGT GTCGGGTGAA GATACCCGCG
GCCCTGCCTG TGGCAGCCGC CCCCTATCCT GGGCTGGTGG AGACCGGAGT GGCTGGAACT
CTGGGTGGCG GAGCCGCTTT GGGGTCAGAG TTCCTAGGAG CCGGGTCTGT GGCAGGGGCA
CTGGGGGGAG CTGGACTGAC AGGGGGAGGT ACTGCTGCTG GCGTAGCTGG TGCTGCTGCT
GGCGTGGCCG GTGCTGCTGT TGCTGGACCT AGTGGAGACA TGGCTCTCAC CAAACTGCCC
ACTTCGTTGC TTGCTGAGAC TCTCGGGCCA GGCGGCGGTT TTCCCCCTCT GCCCCCTCCC
CCTTACCTGC CCCCTTTGGG GGCGGGCCTC GGGACAGTGG ACGAAGGTGA CTCTCTGGAT
GGACCAGAAT ACGAGGAGGA AGAGGTGGCC ATACCGTTGA CCGCTCCTCC AACTAACCAG
TGGTATCACG GAAAACTTGA CAGAACGATA GCAGAAGAAC GCCTCAGGCA GGCAGGGAAG
TCTGGCAGTT ATCTTATAAG AGAGAGTGAT CGGAGGCCAG GGTCCTTTGT ACTTTCATTT
CTTAGCCAGA TGAATGTTGT CAACCATTTT AGGATTATTG CTATGTGTGG AGATTACTAC
ATTGGTGGAA GACGTTTTTC TTCACTGTCA GACCTAATAG GTTATTACAG TCATGTTTCT
TGTTTGCTTA AAGGAGAAAA ATTACTTTAC CCAGTTGCAC CACCAGAGCC AGTAGAAGAT
AGAAGGCGTG TACGAGCTAT TCTACCTTAC ACAAAAGTAC CAGACACTGA TGAAATAAGT
TTCTTAAAAG GAGATATGTT CATTGTTCAT AATGAATTAG AAGATGGATG GATGTGGGTT
ACAAATTTAA GAACAGATGA ACAAGGCCTT ATTGTTGAAG ACCTAGTAGA AGAGGTGGGC
CGGGAAGAAG ATCCACATGA AGGAAAAATA TGGTTCCATG GGAAGATTTC CAAACAGGAA
GCTTATAATT TACTAATGAC AGTTGGTCAA GTCTGCAGTT TTCTTGTGAG GCCCTCAGAT
AATACTCCTG GCGATTATTC ACTTTATTTC CGGACCAATG AAAATATTCA GCGATTTAAA
ATATGTCCAA CGCCAAACAA TCAGTTTATG ATGGGAGGCC GGTATTATAA CAGCATTGGG
GACATCATAG ATCACTATCG AAAAGAACAG ATTGTTGAAG GATATTATCT TAAGGAACCT
GTACCAATGC AGGATCAAGA ACAAGTACTC AATGACACAG TGGATGGCAA GGAAATCTAT
AATACCATCC GTCGTAAAAC AAAGGATGCC TTTTATAAAA ACATTGTTAA GAAAGGTTAT
CTTCTGAAAA AGGGCAAAGG AAAACGTTGG AAAAATTTAT ATTTTATCTT AGAGGGTAGT
GATGCCCAAC TTATTTATTT TGAAAGCGAA AAACGAGCTA CCAAACCAAA AGGATTAATA
GATCTCAGTG TATGTTCTGT CTATGTCGTT CATGATAGTC TCTTTGGCAG GCCAAACTGT
TTTCAGATAG TAGTTCAGCA CTTTAGTGAA GAACATTACA TCTTTTACTT TGCAGGAGAA
ACTCCAGAAC AAGCAGAGGA TTGGATGAAA GGTCTGCAGG CATTTTGCAA TTTACGGAAA
AGTAGTCCAG GGACATCCAA TAAACGCCTT CGTCAGGTCA GCAGCCTTGT TTTACATATT
GAAGAAGCCC ATAAACTCCC AGTAAAACAT TTTACTAATC CATATTGTAA CATCTACCTG
AATAGTGTCC AAGTAGCAAA AACTCATGCA AGGGAAGGGC AAAACCCAGT ATGGTCAGAA
GAGTTTGTCT TTGATGATCT TCCTCCTGAC ATCAATAGAT TTGAAATAAC TCTTAGTAAT
AAAACAAAGA AAAGCAAAGA TCCTGATATC TTATTTATGC GCTGCCAGTT GAGCCGATTA
CAGAAAGGGC ATGCCACAGA TGAATGGTTT CTGCTCAGCT CCCATATACC ATTAAAAGGT
ATTGAACCAG GGTCCCTGCG TGTTCGAGCA CGATACTCTA TGGAAAAAAT CATGCCAGAA
GAAGAGTACA GTGAATTTAA AGAGCTTATA CTGCAAAAGG AACTTCATGT AGTCTATGCT
TTATCACATG TATGTGGACA AGACCGAACA CTACTGGCCA GCATCCTACT GAGGATTTTT
CTTCACGAAA AGCTTGAATC GTTGTTGTTA TGCACACTAA ATGACAGAGA AATAAGCATG
GAAGATGAAG CCACTACCCT ATTTCGAGCC ACAACACTTG CAAGCACCTT GATGGAGCAG
TATATGAAAG CCACTGCTAC ACAGTTTGTT CATCATGCTT TGAAAGACTC TATTTTAAAG
ATAATGGAAA GCAAGCAGTC TTGTGAGTTA AGTCCATCAA AGTTAGAAAA AAATGAAGAT
GTGAACACTA ATTTAACACA CCTATTGAAC ATACTTTCAG AGCTTGTGGA GAAAATATTC
ATGGCTTCAG AAATACTTCC ACCGACATTG AGATATATTT ATGGGTGTTT ACAGAAATCT
GTTCAGCATA AGTGGCCTAC AAATACCACC ATGAGAACAA GAGTTGTTAG TGGTTTTGTT
TTTCTTCGAC TCATCTGTCC TGCCATCCTG AATCCACGGA TGTTCAATAT CATCTCAGAT
TCTCCATCTC CTATTGCTGC AAGAACACTG ATATTAGTGG CTAAATCTGT GCAGAACTTA
GCAAATCTTG TGGAATTTGG AGCTAAGGAG CCCTACATGG AAGGTGTCAA TCCATTCATC
AAAAGCAACA AACATCGTAT GATCATGTTT TTAGATGAAC TTGGGCTTTT TTACTTTGCT
GCTACTATGT GGGGCGTGAA GCAAGACGAT GACACAGTTC TGACCAGGTT AAATAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60199
Clone ID Related Accession (Same CDS sequence) XM_011543527
Accession Version XM_011543527.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1620bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product ras GTPase-activating protein 1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_034772.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGATGGCGG CCGAGGCCGG CAGTGAGGAG GGCGGCCCGG TAACAGCCGG AGCTGGAGGA 
GGCGGCGCGG CAGCGGGCTC CAGTGCCTAT CCCGCAGTGT GTCGGGTGAA GATACCCGCG
GCCCTGCCTG TGGCAGCCGC CCCCTATCCT GGGCTGGTGG AGACCGGAGT GGCTGGAACT
CTGGGTGGCG GAGCCGCTTT GGGGTCAGAG TTCCTAGGAG CCGGGTCTGT GGCAGGGGCA
CTGGGGGGAG CTGGACTGAC AGGGGGAGGT ACTGCTGCTG GCGTAGCTGG TGCTGCTGCT
GGCGTGGCCG GTGCTGCTGT TGCTGGACCT AGTGGAGACA TGGCTCTCAC CAAACTGCCC
ACTTCGTTGC TTGCTGAGAC TCTCGGGCCA GGCGGCGGTT TTCCCCCTCT GCCCCCTCCC
CCTTACCTGC CCCCTTTGGG GGCGGGCCTC GGGACAGTGG ACGAAGGTGA CTCTCTGGAT
GGACCAGAAT ACGAGGAGGA AGAGGTGGCC ATACCGTTGA CCGCTCCTCC AACTAACCAG
TGGTATCACG GAAAACTTGA CAGAACGATA GCAGAAGAAC GCCTCAGGCA GGCAGGGAAG
TCTGGCAGTT ATCTTATAAG AGAGAGTGAT CGGAGGCCAG GGTCCTTTGT ACTTTCATTT
CTTAGCCAGA TGAATGTTGT CAACCATTTT AGGATTATTG CTATGTGTGG AGATTACTAC
ATTGGTGGAA GACGTTTTTC TTCACTGTCA GACCTAATAG GTTATTACAG TCATGTTTCT
TGTTTGCTTA AAGGAGAAAA ATTACTTTAC CCAGTTGCAC CACCAGAGCC AGTAGAAGAT
AGAAGGCGTG TACGAGCTAT TCTACCTTAC ACAAAAGTAC CAGACACTGA TGAAATAAGT
TTCTTAAAAG GAGATATGTT CATTGTTCAT AATGAATTAG AAGATGGATG GATGTGGGTT
ACAAATTTAA GAACAGATGA ACAAGGCCTT ATTGTTGAAG ACCTAGTAGA AGAGGTGGGC
CGGGAAGAAG ATCCACATGA AGGAAAAATA TGGTTCCATG GGAAGATTTC CAAACAGGAA
GCTTATAATT TACTAATGAC AGTTGGTCAA GTCTGCAGTT TTCTTGTGAG GCCCTCAGAT
AATACTCCTG GCGATTATTC ACTTTATTTC CGGACCAATG AAAATATTCA GCGATTTAAA
ATATGTCCAA CGCCAAACAA TCAGTTTATG ATGGGAGGCC GGTATTATAA CAGCATTGGG
GACATCATAG ATCACTATCG AAAAGAACAG ATTGTTGAAG GATATTATCT TAAGGAACCT
GTACCAATGC AGGATCAAGA ACAAGTACTC AATGACACAG TGGATGGCAA GGAAATCTAT
AATACCATCC GTCGTAAAAC AAAGGATGCC TTTTATAAAA ACATTGTTAA GAAAGGTTAT
CTTCTGAAAA AGGGCAAAGG AAAACGTTGG AAAAATTTAT ATTTTATCTT AGAGGGTAGT
GATGCCCAAC TTATTTATTT TGAAAGCGAA AAACGAGCTA CCAAACCAAA AGGATTAATA
GATCTCAGTG TATGTTCTGT CTATGTCGTT CATGATAGTC TCTTTGGCAG TTTTAGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011541829.1
CDS652..2271
Misc Feature(1)1132..1443(+)
Misc Feature(2)1213..1344(+)
Misc Feature(3)1339..1419(+)
Misc Feature(4)1492..1668(+)
Misc Feature(5)1699..1929(+)
Misc Feature(6)1723..1851(+)
Misc Feature(7)1846..1929(+)
Misc Feature(8)2116..>2259(+)
Translation

Target ORF information:

RefSeq Version XM_011543527
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543527

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGATGGCGG CCGAGGCCGG CAGTGAGGAG GGCGGCCCGG TAACAGCCGG AGCTGGAGGA 
GGCGGCGCGG CAGCGGGCTC CAGTGCCTAT CCCGCAGTGT GTCGGGTGAA GATACCCGCG
GCCCTGCCTG TGGCAGCCGC CCCCTATCCT GGGCTGGTGG AGACCGGAGT GGCTGGAACT
CTGGGTGGCG GAGCCGCTTT GGGGTCAGAG TTCCTAGGAG CCGGGTCTGT GGCAGGGGCA
CTGGGGGGAG CTGGACTGAC AGGGGGAGGT ACTGCTGCTG GCGTAGCTGG TGCTGCTGCT
GGCGTGGCCG GTGCTGCTGT TGCTGGACCT AGTGGAGACA TGGCTCTCAC CAAACTGCCC
ACTTCGTTGC TTGCTGAGAC TCTCGGGCCA GGCGGCGGTT TTCCCCCTCT GCCCCCTCCC
CCTTACCTGC CCCCTTTGGG GGCGGGCCTC GGGACAGTGG ACGAAGGTGA CTCTCTGGAT
GGACCAGAAT ACGAGGAGGA AGAGGTGGCC ATACCGTTGA CCGCTCCTCC AACTAACCAG
TGGTATCACG GAAAACTTGA CAGAACGATA GCAGAAGAAC GCCTCAGGCA GGCAGGGAAG
TCTGGCAGTT ATCTTATAAG AGAGAGTGAT CGGAGGCCAG GGTCCTTTGT ACTTTCATTT
CTTAGCCAGA TGAATGTTGT CAACCATTTT AGGATTATTG CTATGTGTGG AGATTACTAC
ATTGGTGGAA GACGTTTTTC TTCACTGTCA GACCTAATAG GTTATTACAG TCATGTTTCT
TGTTTGCTTA AAGGAGAAAA ATTACTTTAC CCAGTTGCAC CACCAGAGCC AGTAGAAGAT
AGAAGGCGTG TACGAGCTAT TCTACCTTAC ACAAAAGTAC CAGACACTGA TGAAATAAGT
TTCTTAAAAG GAGATATGTT CATTGTTCAT AATGAATTAG AAGATGGATG GATGTGGGTT
ACAAATTTAA GAACAGATGA ACAAGGCCTT ATTGTTGAAG ACCTAGTAGA AGAGGTGGGC
CGGGAAGAAG ATCCACATGA AGGAAAAATA TGGTTCCATG GGAAGATTTC CAAACAGGAA
GCTTATAATT TACTAATGAC AGTTGGTCAA GTCTGCAGTT TTCTTGTGAG GCCCTCAGAT
AATACTCCTG GCGATTATTC ACTTTATTTC CGGACCAATG AAAATATTCA GCGATTTAAA
ATATGTCCAA CGCCAAACAA TCAGTTTATG ATGGGAGGCC GGTATTATAA CAGCATTGGG
GACATCATAG ATCACTATCG AAAAGAACAG ATTGTTGAAG GATATTATCT TAAGGAACCT
GTACCAATGC AGGATCAAGA ACAAGTACTC AATGACACAG TGGATGGCAA GGAAATCTAT
AATACCATCC GTCGTAAAAC AAAGGATGCC TTTTATAAAA ACATTGTTAA GAAAGGTTAT
CTTCTGAAAA AGGGCAAAGG AAAACGTTGG AAAAATTTAT ATTTTATCTT AGAGGGTAGT
GATGCCCAAC TTATTTATTT TGAAAGCGAA AAACGAGCTA CCAAACCAAA AGGATTAATA
GATCTCAGTG TATGTTCTGT CTATGTCGTT CATGATAGTC TCTTTGGCAG TTTTAGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18040
Clone ID Related Accession (Same CDS sequence) NM_002890
Accession Version NM_002890.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3144bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ras GTPase-activating protein 1 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB209106.1, M23379.1 and CF528258.1. This sequence is a reference standard in the RefSeqGene project. On Sep 20, 2011 this sequence version replaced gi:4506430. Transcript Variant: This variant (1) lacks the alternatively spliced insert found in variant 2, resulting in translation of the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209106.1, BC033015.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGATGGCGG CCGAGGCCGG CAGTGAGGAG GGCGGCCCGG TAACAGCCGG AGCTGGAGGA 
GGCGGCGCGG CAGCGGGCTC CAGTGCCTAT CCCGCAGTGT GTCGGGTGAA GATACCCGCG
GCCCTGCCTG TGGCAGCCGC CCCCTATCCT GGGCTGGTGG AGACCGGAGT GGCTGGAACT
CTGGGTGGCG GAGCCGCTTT GGGGTCAGAG TTCCTAGGAG CCGGGTCTGT GGCAGGGGCA
CTGGGGGGAG CTGGACTGAC AGGGGGAGGT ACTGCTGCTG GCGTAGCTGG TGCTGCTGCT
GGCGTGGCCG GTGCTGCTGT TGCTGGACCT AGTGGAGACA TGGCTCTCAC CAAACTGCCC
ACTTCGTTGC TTGCTGAGAC TCTCGGGCCA GGCGGCGGTT TTCCCCCTCT GCCCCCTCCC
CCTTACCTGC CCCCTTTGGG GGCGGGCCTC GGGACAGTGG ACGAAGGTGA CTCTCTGGAT
GGACCAGAAT ACGAGGAGGA AGAGGTGGCC ATACCGTTGA CCGCTCCTCC AACTAACCAG
TGGTATCACG GAAAACTTGA CAGAACGATA GCAGAAGAAC GCCTCAGGCA GGCAGGGAAG
TCTGGCAGTT ATCTTATAAG AGAGAGTGAT CGGAGGCCAG GGTCCTTTGT ACTTTCATTT
CTTAGCCAGA TGAATGTTGT CAACCATTTT AGGATTATTG CTATGTGTGG AGATTACTAC
ATTGGTGGAA GACGTTTTTC TTCACTGTCA GACCTAATAG GTTATTACAG TCATGTTTCT
TGTTTGCTTA AAGGAGAAAA ATTACTTTAC CCAGTTGCAC CACCAGAGCC AGTAGAAGAT
AGAAGGCGTG TACGAGCTAT TCTACCTTAC ACAAAAGTAC CAGACACTGA TGAAATAAGT
TTCTTAAAAG GAGATATGTT CATTGTTCAT AATGAATTAG AAGATGGATG GATGTGGGTT
ACAAATTTAA GAACAGATGA ACAAGGCCTT ATTGTTGAAG ACCTAGTAGA AGAGGTGGGC
CGGGAAGAAG ATCCACATGA AGGAAAAATA TGGTTCCATG GGAAGATTTC CAAACAGGAA
GCTTATAATT TACTAATGAC AGTTGGTCAA GTCTGCAGTT TTCTTGTGAG GCCCTCAGAT
AATACTCCTG GCGATTATTC ACTTTATTTC CGGACCAATG AAAATATTCA GCGATTTAAA
ATATGTCCAA CGCCAAACAA TCAGTTTATG ATGGGAGGCC GGTATTATAA CAGCATTGGG
GACATCATAG ATCACTATCG AAAAGAACAG ATTGTTGAAG GATATTATCT TAAGGAACCT
GTACCAATGC AGGATCAAGA ACAAGTACTC AATGACACAG TGGATGGCAA GGAAATCTAT
AATACCATCC GTCGTAAAAC AAAGGATGCC TTTTATAAAA ACATTGTTAA GAAAGGTTAT
CTTCTGAAAA AGGGCAAAGG AAAACGTTGG AAAAATTTAT ATTTTATCTT AGAGGGTAGT
GATGCCCAAC TTATTTATTT TGAAAGCGAA AAACGAGCTA CCAAACCAAA AGGATTAATA
GATCTCAGTG TATGTTCTGT CTATGTCGTT CATGATAGTC TCTTTGGCAG GCCAAACTGT
TTTCAGATAG TAGTTCAGCA CTTTAGTGAA GAACATTACA TCTTTTACTT TGCAGGAGAA
ACTCCAGAAC AAGCAGAGGA TTGGATGAAA GGTCTGCAGG CATTTTGCAA TTTACGGAAA
AGTAGTCCAG GGACATCCAA TAAACGCCTT CGTCAGGTCA GCAGCCTTGT TTTACATATT
GAAGAAGCCC ATAAACTCCC AGTAAAACAT TTTACTAATC CATATTGTAA CATCTACCTG
AATAGTGTCC AAGTAGCAAA AACTCATGCA AGGGAAGGGC AAAACCCAGT ATGGTCAGAA
GAGTTTGTCT TTGATGATCT TCCTCCTGAC ATCAATAGAT TTGAAATAAC TCTTAGTAAT
AAAACAAAGA AAAGCAAAGA TCCTGATATC TTATTTATGC GCTGCCAGTT GAGCCGATTA
CAGAAAGGGC ATGCCACAGA TGAATGGTTT CTGCTCAGCT CCCATATACC ATTAAAAGGT
ATTGAACCAG GGTCCCTGCG TGTTCGAGCA CGATACTCTA TGGAAAAAAT CATGCCAGAA
GAAGAGTACA GTGAATTTAA AGAGCTTATA CTGCAAAAGG AACTTCATGT AGTCTATGCT
TTATCACATG TATGTGGACA AGACCGAACA CTACTGGCCA GCATCCTACT GAGGATTTTT
CTTCACGAAA AGCTTGAATC GTTGTTGTTA TGCACACTAA ATGACAGAGA AATAAGCATG
GAAGATGAAG CCACTACCCT ATTTCGAGCC ACAACACTTG CAAGCACCTT GATGGAGCAG
TATATGAAAG CCACTGCTAC ACAGTTTGTT CATCATGCTT TGAAAGACTC TATTTTAAAG
ATAATGGAAA GCAAGCAGTC TTGTGAGTTA AGTCCATCAA AGTTAGAAAA AAATGAAGAT
GTGAACACTA ATTTAACACA CCTATTGAAC ATACTTTCAG AGCTTGTGGA GAAAATATTC
ATGGCTTCAG AAATACTTCC ACCGACATTG AGATATATTT ATGGGTGTTT ACAGAAATCT
GTTCAGCATA AGTGGCCTAC AAATACCACC ATGAGAACAA GAGTTGTTAG TGGTTTTGTT
TTTCTTCGAC TCATCTGTCC TGCCATCCTG AATCCACGGA TGTTCAATAT CATCTCAGAT
TCTCCATCTC CTATTGCTGC AAGAACACTG ATATTAGTGG CTAAATCTGT GCAGAACTTA
GCAAATCTTG TGGAATTTGG AGCTAAGGAG CCCTACATGG AAGGTGTCAA TCCATTCATC
AAAAGCAACA AACATCGTAT GATCATGTTT TTAGATGAAC TTGGGAATGT ACCTGAACTT
CCGGACACTA CAGAGCATTC TAGAACGGAC CTGTCCCGTG ATTTAGCAGC ATTGCATGAG
ATTTGCGTGG CTCATTCAGA TGAACTTCGA ACGCTCAGTA ATGAGCGTGG TGCACAGCAG
CACGTATTGA AAAAGCTTCT GGCTATAACA GAACTGCTTC AACAAAAACA AAACCAGTAT
ACAAAAACCA ATGATGTCAG GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002881.1
CDS200..3343
Misc Feature(1)98..100(+)
Misc Feature(2)200..202(+)
Misc Feature(3)668..670(+)
Misc Feature(4)668..670(+)
Misc Feature(5)680..991(+)
Misc Feature(6)761..892(+)
Misc Feature(7)887..967(+)
Misc Feature(8)1040..1216(+)
Misc Feature(9)1247..1477(+)
Misc Feature(10)1271..1399(+)
Misc Feature(11)1394..1477(+)
Misc Feature(12)1562..1564(+)
Misc Feature(13)1562..1564(+)
Misc Feature(14)1577..1579(+)
Misc Feature(15)1577..1579(+)
Misc Feature(16)1664..1927(+)
Misc Feature(17)1970..2347(+)
Misc Feature(18)2042..2044(+)
Misc Feature(19)2042..2044(+)
Misc Feature(20)2042..2044(+)
Misc Feature(21)2291..3331(+)
Misc Feature(22)2339..3334(+)
Misc Feature(23)2444..3049(+)
Exon (1)1..738
Gene:RASA1
Gene Synonym:
Exon (2)739..891
Gene:RASA1
Gene Synonym:
Exon (3)892..1027
Gene:RASA1
Gene Synonym:
Exon (4)1028..1098
Gene:RASA1
Gene Synonym:
Exon (5)1099..1216
Gene:RASA1
Gene Synonym:
Exon (6)1217..1248
Gene:RASA1
Gene Synonym:
Exon (7)1249..1301
Gene:RASA1
Gene Synonym:
Exon (8)1302..1452
Gene:RASA1
Gene Synonym:
Exon (9)1453..1531
Gene:RASA1
Gene Synonym:
Exon (10)1532..1652
Gene:RASA1
Gene Synonym:
Exon (11)1653..1809
Gene:RASA1
Gene Synonym:
Exon (12)1810..1897
Gene:RASA1
Gene Synonym:
Exon (13)1898..1975
Gene:RASA1
Gene Synonym:
Exon (14)1976..2133
Gene:RASA1
Gene Synonym:
Exon (15)2134..2210
Gene:RASA1
Gene Synonym:
Exon (16)2211..2383
Gene:RASA1
Gene Synonym:
Exon (17)2384..2543
Gene:RASA1
Gene Synonym:
Exon (18)2544..2686
Gene:RASA1
Gene Synonym:
Exon (19)2687..2802
Gene:RASA1
Gene Synonym:
Exon (20)2803..2889
Gene:RASA1
Gene Synonym:
Exon (21)2890..2957
Gene:RASA1
Gene Synonym:
Exon (22)2958..3046
Gene:RASA1
Gene Synonym:
Exon (23)3047..3124
Gene:RASA1
Gene Synonym:
Exon (24)3125..3259
Gene:RASA1
Gene Synonym:
Exon (25)3260..4386
Gene:RASA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002890
Organism Homo sapiens (human)
Definition Homo sapiens RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002890

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGATGGCGG CCGAGGCCGG CAGTGAGGAG GGCGGCCCGG TAACAGCCGG AGCTGGAGGA 
GGCGGCGCGG CAGCGGGCTC CAGTGCCTAT CCCGCAGTGT GTCGGGTGAA GATACCCGCG
GCCCTGCCTG TGGCAGCCGC CCCCTATCCT GGGCTGGTGG AGACCGGAGT GGCTGGAACT
CTGGGTGGCG GAGCCGCTTT GGGGTCAGAG TTCCTAGGAG CCGGGTCTGT GGCAGGGGCA
CTGGGGGGAG CTGGACTGAC AGGGGGAGGT ACTGCTGCTG GCGTAGCTGG TGCTGCTGCT
GGCGTGGCCG GTGCTGCTGT TGCTGGACCT AGTGGAGACA TGGCTCTCAC CAAACTGCCC
ACTTCGTTGC TTGCTGAGAC TCTCGGGCCA GGCGGCGGTT TTCCCCCTCT GCCCCCTCCC
CCTTACCTGC CCCCTTTGGG GGCGGGCCTC GGGACAGTGG ACGAAGGTGA CTCTCTGGAT
GGACCAGAAT ACGAGGAGGA AGAGGTGGCC ATACCGTTGA CCGCTCCTCC AACTAACCAG
TGGTATCACG GAAAACTTGA CAGAACGATA GCAGAAGAAC GCCTCAGGCA GGCAGGGAAG
TCTGGCAGTT ATCTTATAAG AGAGAGTGAT CGGAGGCCAG GGTCCTTTGT ACTTTCATTT
CTTAGCCAGA TGAATGTTGT CAACCATTTT AGGATTATTG CTATGTGTGG AGATTACTAC
ATTGGTGGAA GACGTTTTTC TTCACTGTCA GACCTAATAG GTTATTACAG TCATGTTTCT
TGTTTGCTTA AAGGAGAAAA ATTACTTTAC CCAGTTGCAC CACCAGAGCC AGTAGAAGAT
AGAAGGCGTG TACGAGCTAT TCTACCTTAC ACAAAAGTAC CAGACACTGA TGAAATAAGT
TTCTTAAAAG GAGATATGTT CATTGTTCAT AATGAATTAG AAGATGGATG GATGTGGGTT
ACAAATTTAA GAACAGATGA ACAAGGCCTT ATTGTTGAAG ACCTAGTAGA AGAGGTGGGC
CGGGAAGAAG ATCCACATGA AGGAAAAATA TGGTTCCATG GGAAGATTTC CAAACAGGAA
GCTTATAATT TACTAATGAC AGTTGGTCAA GTCTGCAGTT TTCTTGTGAG GCCCTCAGAT
AATACTCCTG GCGATTATTC ACTTTATTTC CGGACCAATG AAAATATTCA GCGATTTAAA
ATATGTCCAA CGCCAAACAA TCAGTTTATG ATGGGAGGCC GGTATTATAA CAGCATTGGG
GACATCATAG ATCACTATCG AAAAGAACAG ATTGTTGAAG GATATTATCT TAAGGAACCT
GTACCAATGC AGGATCAAGA ACAAGTACTC AATGACACAG TGGATGGCAA GGAAATCTAT
AATACCATCC GTCGTAAAAC AAAGGATGCC TTTTATAAAA ACATTGTTAA GAAAGGTTAT
CTTCTGAAAA AGGGCAAAGG AAAACGTTGG AAAAATTTAT ATTTTATCTT AGAGGGTAGT
GATGCCCAAC TTATTTATTT TGAAAGCGAA AAACGAGCTA CCAAACCAAA AGGATTAATA
GATCTCAGTG TATGTTCTGT CTATGTCGTT CATGATAGTC TCTTTGGCAG GCCAAACTGT
TTTCAGATAG TAGTTCAGCA CTTTAGTGAA GAACATTACA TCTTTTACTT TGCAGGAGAA
ACTCCAGAAC AAGCAGAGGA TTGGATGAAA GGTCTGCAGG CATTTTGCAA TTTACGGAAA
AGTAGTCCAG GGACATCCAA TAAACGCCTT CGTCAGGTCA GCAGCCTTGT TTTACATATT
GAAGAAGCCC ATAAACTCCC AGTAAAACAT TTTACTAATC CATATTGTAA CATCTACCTG
AATAGTGTCC AAGTAGCAAA AACTCATGCA AGGGAAGGGC AAAACCCAGT ATGGTCAGAA
GAGTTTGTCT TTGATGATCT TCCTCCTGAC ATCAATAGAT TTGAAATAAC TCTTAGTAAT
AAAACAAAGA AAAGCAAAGA TCCTGATATC TTATTTATGC GCTGCCAGTT GAGCCGATTA
CAGAAAGGGC ATGCCACAGA TGAATGGTTT CTGCTCAGCT CCCATATACC ATTAAAAGGT
ATTGAACCAG GGTCCCTGCG TGTTCGAGCA CGATACTCTA TGGAAAAAAT CATGCCAGAA
GAAGAGTACA GTGAATTTAA AGAGCTTATA CTGCAAAAGG AACTTCATGT AGTCTATGCT
TTATCACATG TATGTGGACA AGACCGAACA CTACTGGCCA GCATCCTACT GAGGATTTTT
CTTCACGAAA AGCTTGAATC GTTGTTGTTA TGCACACTAA ATGACAGAGA AATAAGCATG
GAAGATGAAG CCACTACCCT ATTTCGAGCC ACAACACTTG CAAGCACCTT GATGGAGCAG
TATATGAAAG CCACTGCTAC ACAGTTTGTT CATCATGCTT TGAAAGACTC TATTTTAAAG
ATAATGGAAA GCAAGCAGTC TTGTGAGTTA AGTCCATCAA AGTTAGAAAA AAATGAAGAT
GTGAACACTA ATTTAACACA CCTATTGAAC ATACTTTCAG AGCTTGTGGA GAAAATATTC
ATGGCTTCAG AAATACTTCC ACCGACATTG AGATATATTT ATGGGTGTTT ACAGAAATCT
GTTCAGCATA AGTGGCCTAC AAATACCACC ATGAGAACAA GAGTTGTTAG TGGTTTTGTT
TTTCTTCGAC TCATCTGTCC TGCCATCCTG AATCCACGGA TGTTCAATAT CATCTCAGAT
TCTCCATCTC CTATTGCTGC AAGAACACTG ATATTAGTGG CTAAATCTGT GCAGAACTTA
GCAAATCTTG TGGAATTTGG AGCTAAGGAG CCCTACATGG AAGGTGTCAA TCCATTCATC
AAAAGCAACA AACATCGTAT GATCATGTTT TTAGATGAAC TTGGGAATGT ACCTGAACTT
CCGGACACTA CAGAGCATTC TAGAACGGAC CTGTCCCGTG ATTTAGCAGC ATTGCATGAG
ATTTGCGTGG CTCATTCAGA TGAACTTCGA ACGCTCAGTA ATGAGCGTGG TGCACAGCAG
CACGTATTGA AAAAGCTTCT GGCTATAACA GAACTGCTTC AACAAAAACA AAACCAGTAT
ACAAAAACCA ATGATGTCAG GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16849
Clone ID Related Accession (Same CDS sequence) NM_022650
Accession Version NM_022650.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2613bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ras GTPase-activating protein 1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA863496.1, M23612.1 and CF528258.1. On Sep 20, 2011 this sequence version replaced gi:12545405. Transcript Variant: This variant (2) includes an alternatively spliced exon in the 5' portion of the transcript, allowing for translation from an alternative initiation codon and resulting in the shorter isoform (2) which is missing the hydrophobic amino terminus found in isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC070063.1, M23612.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGAAGGGGT GGTATCACGG AAAACTTGAC AGAACGATAG CAGAAGAACG CCTCAGGCAG 
GCAGGGAAGT CTGGCAGTTA TCTTATAAGA GAGAGTGATC GGAGGCCAGG GTCCTTTGTA
CTTTCATTTC TTAGCCAGAT GAATGTTGTC AACCATTTTA GGATTATTGC TATGTGTGGA
GATTACTACA TTGGTGGAAG ACGTTTTTCT TCACTGTCAG ACCTAATAGG TTATTACAGT
CATGTTTCTT GTTTGCTTAA AGGAGAAAAA TTACTTTACC CAGTTGCACC ACCAGAGCCA
GTAGAAGATA GAAGGCGTGT ACGAGCTATT CTACCTTACA CAAAAGTACC AGACACTGAT
GAAATAAGTT TCTTAAAAGG AGATATGTTC ATTGTTCATA ATGAATTAGA AGATGGATGG
ATGTGGGTTA CAAATTTAAG AACAGATGAA CAAGGCCTTA TTGTTGAAGA CCTAGTAGAA
GAGGTGGGCC GGGAAGAAGA TCCACATGAA GGAAAAATAT GGTTCCATGG GAAGATTTCC
AAACAGGAAG CTTATAATTT ACTAATGACA GTTGGTCAAG TCTGCAGTTT TCTTGTGAGG
CCCTCAGATA ATACTCCTGG CGATTATTCA CTTTATTTCC GGACCAATGA AAATATTCAG
CGATTTAAAA TATGTCCAAC GCCAAACAAT CAGTTTATGA TGGGAGGCCG GTATTATAAC
AGCATTGGGG ACATCATAGA TCACTATCGA AAAGAACAGA TTGTTGAAGG ATATTATCTT
AAGGAACCTG TACCAATGCA GGATCAAGAA CAAGTACTCA ATGACACAGT GGATGGCAAG
GAAATCTATA ATACCATCCG TCGTAAAACA AAGGATGCCT TTTATAAAAA CATTGTTAAG
AAAGGTTATC TTCTGAAAAA GGGCAAAGGA AAACGTTGGA AAAATTTATA TTTTATCTTA
GAGGGTAGTG ATGCCCAACT TATTTATTTT GAAAGCGAAA AACGAGCTAC CAAACCAAAA
GGATTAATAG ATCTCAGTGT ATGTTCTGTC TATGTCGTTC ATGATAGTCT CTTTGGCAGG
CCAAACTGTT TTCAGATAGT AGTTCAGCAC TTTAGTGAAG AACATTACAT CTTTTACTTT
GCAGGAGAAA CTCCAGAACA AGCAGAGGAT TGGATGAAAG GTCTGCAGGC ATTTTGCAAT
TTACGGAAAA GTAGTCCAGG GACATCCAAT AAACGCCTTC GTCAGGTCAG CAGCCTTGTT
TTACATATTG AAGAAGCCCA TAAACTCCCA GTAAAACATT TTACTAATCC ATATTGTAAC
ATCTACCTGA ATAGTGTCCA AGTAGCAAAA ACTCATGCAA GGGAAGGGCA AAACCCAGTA
TGGTCAGAAG AGTTTGTCTT TGATGATCTT CCTCCTGACA TCAATAGATT TGAAATAACT
CTTAGTAATA AAACAAAGAA AAGCAAAGAT CCTGATATCT TATTTATGCG CTGCCAGTTG
AGCCGATTAC AGAAAGGGCA TGCCACAGAT GAATGGTTTC TGCTCAGCTC CCATATACCA
TTAAAAGGTA TTGAACCAGG GTCCCTGCGT GTTCGAGCAC GATACTCTAT GGAAAAAATC
ATGCCAGAAG AAGAGTACAG TGAATTTAAA GAGCTTATAC TGCAAAAGGA ACTTCATGTA
GTCTATGCTT TATCACATGT ATGTGGACAA GACCGAACAC TACTGGCCAG CATCCTACTG
AGGATTTTTC TTCACGAAAA GCTTGAATCG TTGTTGTTAT GCACACTAAA TGACAGAGAA
ATAAGCATGG AAGATGAAGC CACTACCCTA TTTCGAGCCA CAACACTTGC AAGCACCTTG
ATGGAGCAGT ATATGAAAGC CACTGCTACA CAGTTTGTTC ATCATGCTTT GAAAGACTCT
ATTTTAAAGA TAATGGAAAG CAAGCAGTCT TGTGAGTTAA GTCCATCAAA GTTAGAAAAA
AATGAAGATG TGAACACTAA TTTAACACAC CTATTGAACA TACTTTCAGA GCTTGTGGAG
AAAATATTCA TGGCTTCAGA AATACTTCCA CCGACATTGA GATATATTTA TGGGTGTTTA
CAGAAATCTG TTCAGCATAA GTGGCCTACA AATACCACCA TGAGAACAAG AGTTGTTAGT
GGTTTTGTTT TTCTTCGACT CATCTGTCCT GCCATCCTGA ATCCACGGAT GTTCAATATC
ATCTCAGATT CTCCATCTCC TATTGCTGCA AGAACACTGA TATTAGTGGC TAAATCTGTG
CAGAACTTAG CAAATCTTGT GGAATTTGGA GCTAAGGAGC CCTACATGGA AGGTGTCAAT
CCATTCATCA AAAGCAACAA ACATCGTATG ATCATGTTTT TAGATGAACT TGGGAATGTA
CCTGAACTTC CGGACACTAC AGAGCATTCT AGAACGGACC TGTCCCGTGA TTTAGCAGCA
TTGCATGAGA TTTGCGTGGC TCATTCAGAT GAACTTCGAA CGCTCAGTAA TGAGCGTGGT
GCACAGCAGC ACGTATTGAA AAAGCTTCTG GCTATAACAG AACTGCTTCA ACAAAAACAA
AACCAGTATA CAAAAACCAA TGATGTCAGG TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_072179.1
CDS125..2737
Misc Feature(1)74..76(+)
Misc Feature(2)134..385(+)
Misc Feature(3)155..286(+)
Misc Feature(4)281..358(+)
Misc Feature(5)434..610(+)
Misc Feature(6)641..871(+)
Misc Feature(7)665..793(+)
Misc Feature(8)788..871(+)
Misc Feature(9)956..958(+)
Misc Feature(10)956..958(+)
Misc Feature(11)971..973(+)
Misc Feature(12)971..973(+)
Misc Feature(13)1058..1321(+)
Misc Feature(14)1364..1741(+)
Misc Feature(15)1436..1438(+)
Misc Feature(16)1436..1438(+)
Misc Feature(17)1685..2725(+)
Misc Feature(18)1733..2728(+)
Misc Feature(19)1838..2443(+)
Exon (1)1..132
Gene:RASA1
Gene Synonym:
Exon (2)133..285
Gene:RASA1
Gene Synonym:
Exon (3)286..421
Gene:RASA1
Gene Synonym:
Exon (4)422..492
Gene:RASA1
Gene Synonym:
Exon (5)493..610
Gene:RASA1
Gene Synonym:
Exon (6)611..642
Gene:RASA1
Gene Synonym:
Exon (7)643..695
Gene:RASA1
Gene Synonym:
Exon (8)696..846
Gene:RASA1
Gene Synonym:
Exon (9)847..925
Gene:RASA1
Gene Synonym:
Exon (10)926..1046
Gene:RASA1
Gene Synonym:
Exon (11)1047..1203
Gene:RASA1
Gene Synonym:
Exon (12)1204..1291
Gene:RASA1
Gene Synonym:
Exon (13)1292..1369
Gene:RASA1
Gene Synonym:
Exon (14)1370..1527
Gene:RASA1
Gene Synonym:
Exon (15)1528..1604
Gene:RASA1
Gene Synonym:
Exon (16)1605..1777
Gene:RASA1
Gene Synonym:
Exon (17)1778..1937
Gene:RASA1
Gene Synonym:
Exon (18)1938..2080
Gene:RASA1
Gene Synonym:
Exon (19)2081..2196
Gene:RASA1
Gene Synonym:
Exon (20)2197..2283
Gene:RASA1
Gene Synonym:
Exon (21)2284..2351
Gene:RASA1
Gene Synonym:
Exon (22)2352..2440
Gene:RASA1
Gene Synonym:
Exon (23)2441..2518
Gene:RASA1
Gene Synonym:
Exon (24)2519..2653
Gene:RASA1
Gene Synonym:
Exon (25)2654..3780
Gene:RASA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_022650
Organism Homo sapiens (human)
Definition Homo sapiens RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_022650

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGAAGGGGT GGTATCACGG AAAACTTGAC AGAACGATAG CAGAAGAACG CCTCAGGCAG 
GCAGGGAAGT CTGGCAGTTA TCTTATAAGA GAGAGTGATC GGAGGCCAGG GTCCTTTGTA
CTTTCATTTC TTAGCCAGAT GAATGTTGTC AACCATTTTA GGATTATTGC TATGTGTGGA
GATTACTACA TTGGTGGAAG ACGTTTTTCT TCACTGTCAG ACCTAATAGG TTATTACAGT
CATGTTTCTT GTTTGCTTAA AGGAGAAAAA TTACTTTACC CAGTTGCACC ACCAGAGCCA
GTAGAAGATA GAAGGCGTGT ACGAGCTATT CTACCTTACA CAAAAGTACC AGACACTGAT
GAAATAAGTT TCTTAAAAGG AGATATGTTC ATTGTTCATA ATGAATTAGA AGATGGATGG
ATGTGGGTTA CAAATTTAAG AACAGATGAA CAAGGCCTTA TTGTTGAAGA CCTAGTAGAA
GAGGTGGGCC GGGAAGAAGA TCCACATGAA GGAAAAATAT GGTTCCATGG GAAGATTTCC
AAACAGGAAG CTTATAATTT ACTAATGACA GTTGGTCAAG TCTGCAGTTT TCTTGTGAGG
CCCTCAGATA ATACTCCTGG CGATTATTCA CTTTATTTCC GGACCAATGA AAATATTCAG
CGATTTAAAA TATGTCCAAC GCCAAACAAT CAGTTTATGA TGGGAGGCCG GTATTATAAC
AGCATTGGGG ACATCATAGA TCACTATCGA AAAGAACAGA TTGTTGAAGG ATATTATCTT
AAGGAACCTG TACCAATGCA GGATCAAGAA CAAGTACTCA ATGACACAGT GGATGGCAAG
GAAATCTATA ATACCATCCG TCGTAAAACA AAGGATGCCT TTTATAAAAA CATTGTTAAG
AAAGGTTATC TTCTGAAAAA GGGCAAAGGA AAACGTTGGA AAAATTTATA TTTTATCTTA
GAGGGTAGTG ATGCCCAACT TATTTATTTT GAAAGCGAAA AACGAGCTAC CAAACCAAAA
GGATTAATAG ATCTCAGTGT ATGTTCTGTC TATGTCGTTC ATGATAGTCT CTTTGGCAGG
CCAAACTGTT TTCAGATAGT AGTTCAGCAC TTTAGTGAAG AACATTACAT CTTTTACTTT
GCAGGAGAAA CTCCAGAACA AGCAGAGGAT TGGATGAAAG GTCTGCAGGC ATTTTGCAAT
TTACGGAAAA GTAGTCCAGG GACATCCAAT AAACGCCTTC GTCAGGTCAG CAGCCTTGTT
TTACATATTG AAGAAGCCCA TAAACTCCCA GTAAAACATT TTACTAATCC ATATTGTAAC
ATCTACCTGA ATAGTGTCCA AGTAGCAAAA ACTCATGCAA GGGAAGGGCA AAACCCAGTA
TGGTCAGAAG AGTTTGTCTT TGATGATCTT CCTCCTGACA TCAATAGATT TGAAATAACT
CTTAGTAATA AAACAAAGAA AAGCAAAGAT CCTGATATCT TATTTATGCG CTGCCAGTTG
AGCCGATTAC AGAAAGGGCA TGCCACAGAT GAATGGTTTC TGCTCAGCTC CCATATACCA
TTAAAAGGTA TTGAACCAGG GTCCCTGCGT GTTCGAGCAC GATACTCTAT GGAAAAAATC
ATGCCAGAAG AAGAGTACAG TGAATTTAAA GAGCTTATAC TGCAAAAGGA ACTTCATGTA
GTCTATGCTT TATCACATGT ATGTGGACAA GACCGAACAC TACTGGCCAG CATCCTACTG
AGGATTTTTC TTCACGAAAA GCTTGAATCG TTGTTGTTAT GCACACTAAA TGACAGAGAA
ATAAGCATGG AAGATGAAGC CACTACCCTA TTTCGAGCCA CAACACTTGC AAGCACCTTG
ATGGAGCAGT ATATGAAAGC CACTGCTACA CAGTTTGTTC ATCATGCTTT GAAAGACTCT
ATTTTAAAGA TAATGGAAAG CAAGCAGTCT TGTGAGTTAA GTCCATCAAA GTTAGAAAAA
AATGAAGATG TGAACACTAA TTTAACACAC CTATTGAACA TACTTTCAGA GCTTGTGGAG
AAAATATTCA TGGCTTCAGA AATACTTCCA CCGACATTGA GATATATTTA TGGGTGTTTA
CAGAAATCTG TTCAGCATAA GTGGCCTACA AATACCACCA TGAGAACAAG AGTTGTTAGT
GGTTTTGTTT TTCTTCGACT CATCTGTCCT GCCATCCTGA ATCCACGGAT GTTCAATATC
ATCTCAGATT CTCCATCTCC TATTGCTGCA AGAACACTGA TATTAGTGGC TAAATCTGTG
CAGAACTTAG CAAATCTTGT GGAATTTGGA GCTAAGGAGC CCTACATGGA AGGTGTCAAT
CCATTCATCA AAAGCAACAA ACATCGTATG ATCATGTTTT TAGATGAACT TGGGAATGTA
CCTGAACTTC CGGACACTAC AGAGCATTCT AGAACGGACC TGTCCCGTGA TTTAGCAGCA
TTGCATGAGA TTTGCGTGGC TCATTCAGAT GAACTTCGAA CGCTCAGTAA TGAGCGTGGT
GCACAGCAGC ACGTATTGAA AAAGCTTCTG GCTATAACAG AACTGCTTCA ACAAAAACAA
AACCAGTATA CAAAAACCAA TGATGTCAGG TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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