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TRPV4 cDNA ORF clone, Homo sapiens (human)

Gene Symbol TRPV4
Entrez Gene ID 59341
Full Name transient receptor potential cation channel, subfamily V, member 4
Synonyms BCYM3, CMT2C, HMSN2C, OTRPC4, SMAL, SPSMA, SSQTL1, TRP12, VRL2, VROAC
General protein information
Preferred Names
transient receptor potential cation channel subfamily V member 4
Names
transient receptor potential cation channel subfamily V member 4
osm-9-like TRP channel 4
vanilloid receptor-like channel 2
transient receptor potential protein 12
OSM9-like transient receptor potential channel 4
osmosensitive transient receptor potential channel 4
vanilloid receptor-related osmotically activated channel
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12q24.1

Summary This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Mutations in this gene are the cause of spondylometaphyseal and metatropic dysplasia and hereditary motor and sensory neuropathy type IIC. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]. lac of sum
Disorder MIM:

605427

Disorder Html: Brachyolmia type 3, 113500 (3); Spondylometaphyseal dysplasia,

mRNA and Protein(s)

mRNA Protein Name
NM_001177428 NP_001170899 transient receptor potential cation channel subfamily V member 4 isoform e
NM_001177431 NP_001170902 transient receptor potential cation channel subfamily V member 4 isoform d
NM_001177433 NP_001170904 transient receptor potential cation channel subfamily V member 4 isoform c
NM_021625 NP_067638 transient receptor potential cation channel subfamily V member 4 isoform a
NM_147204 NP_671737 transient receptor potential cation channel subfamily V member 4 isoform b
XM_005253918 XP_005253975 transient receptor potential cation channel subfamily V member 4 isoform X2
XM_011538630 XP_011536932 transient receptor potential cation channel subfamily V member 4 isoform X2
XM_011538631 XP_011536933 transient receptor potential cation channel subfamily V member 4 isoform X3
XM_011538632 XP_011536934 transient receptor potential cation channel subfamily V member 4 isoform X1
XM_011538633 XP_011536935 transient receptor potential cation channel subfamily V member 4 isoform X4
XM_011538634 XP_011536936 transient receptor potential cation channel subfamily V member 4 isoform X5
XM_011538635 XP_011536937 transient receptor potential cation channel subfamily V member 4 isoform X6
XM_011538636 XP_011536938 transient receptor potential cation channel subfamily V member 4 isoform X7



Homo sapiens (human) TRPV4 NP_067638.3
Pan troglodytes (chimpanzee) TRPV4 XP_003314024.1
Canis lupus familiaris (dog) TRPV4 NP_001120787.1
Bos taurus (cattle) TRPV4 NP_001179314.1
Mus musculus (house mouse) Trpv4 NP_071300.2
Rattus norvegicus (Norway rat) Trpv4 NP_076460.1
Gallus gallus (chicken) TRPV4 NP_990023.1
Danio rerio (zebrafish) trpv4 NP_001036195.1
Xenopus (Silurana) tropicalis (western clawed frog) trpv4 XP_002932129.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TRPV4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRPV4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu22834 NM_001177428 Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu25097 NM_001177431 Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $594.30
$849.00
OHu27266 NM_001177433 Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu21707
NM_021625 Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu23056 NM_147204 Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu21707
XM_005253918 PREDICTED: Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu21707
XM_011538630 PREDICTED: Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu22834 XM_011538631 PREDICTED: Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu23056 XM_011538632 PREDICTED: Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu27266 XM_011538633 PREDICTED: Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu71031 XM_011538634 PREDICTED: Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu71032 XM_011538635 PREDICTED: Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu71033 XM_011538636 PREDICTED: Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22834
Clone ID Related Accession (Same CDS sequence) NM_001177428 , XM_011538631
Accession Version NM_001177428.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2475bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 18-MAR-2015
Organism Homo sapiens (human)
Product transient receptor potential cation channel subfamily V member 4 isoform e
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA869138.1, DQ059644.1, AC007834.40 and AB032427.1. Transcript Variant: This variant (5) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (c) is shorter, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ059644.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG TGGGGAGGCT TTTCCTCTCT CCTCCCTGGC CAATCTGTTT
GAGGGGGAGG ATGGCTCCCT TTCGCCCTCA CCGGCTGATG CCAGTCGCCC TGCTGGCCCA
GGCGATGGGC GACCAAATCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC
AACCCCATCG ATCTGCTGGA GTCCACCCTA TATGAGTCCT CGGTGGTGCC TGGGCCCAAG
AAAGCACCCA TGGACTCACT GTTTGACTAC GGCACCTATC GTCACCACTC CAGTGACAAC
AAGAGGTGGA GGAAGAAGAT CATAGAGAAG CAGCCGCAGA GCCCCAAAGC CCCTGCCCCT
CAGCCGCCCC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG
GGCTCCACTG CTGACCTGGA CGGGCTGCTC CCATTCTTGC TGACCCACAA GAAACGCCTA
ACTGATGAGG AGTTTCGAGA GCCATCTACG GGGAAGACCT GCCTGCCCAA GGCCTTGCTG
AACCTGAGCA ATGGCCGCAA CGACACCATC CCTGTGCTGC TGGACATCGC GGAGCGCACC
GGCAACATGA GGGAGTTCAT TAACTCGCCC TTCCGTGACA TCTACTATCG AGGGGAGCTG
CCCCTGTCGC TGGCTGCCTG CACCAACCAG CCCCACATTG TCAACTACCT GACGGAGAAC
CCCCACAAGA AGGCGGACAT GCGGCGCCAG GACTCGCGAG GCAACACAGT GCTGCATGCG
CTGGTGGCCA TTGCTGACAA CACCCGTGAG AACACCAAGT TTGTTACCAA GATGTACGAC
CTGCTGCTGC TCAAGTGTGC CCGCCTCTTC CCCGACAGCA ACCTGGAGGC CGTGCTCAAC
AACGACGGCC TCTCGCCCCT CATGATGGCT GCCAAGACGG GCAAGATTGG GATCTTTCAG
CACATCATCC GGCGGGAGGT GACGGATGAG GACACACGGC ACCTGTCCCG CAAGTTCAAG
GACTGGGCCT ATGGGCCAGT GTATTCCTCG CTTTATGACC TCTCCTCCCT GGACACGTGT
GGGGAAGAGG CCTCCGTGCT GGAGATCCTG GTGTACAACA GCAAGATTGA GAACCGCCAC
GAGATGCTGG CTGTGGAGCC CATCAATGAA CTGCTGCGGG ACAAGTGGCG CAAGTTCGGG
GCCGTCTCCT TCTACATCAA CGTGGTCTCC TACCTGTGTG CCATGGTCAT CTTCACTCTC
ACCGCCTACT ACCAGCCGCT GGAGGGCACA CCGCCGTACC CTTACCGCAC CACGGTGGAC
TACCTGCGGC TGGCTGGCGA GGTCATTACG CTCTTCACTG GGGTCCTGTT CTTCTTCACC
AACATCAAAG ACTTGTTCAT GAAGAAATGC CCTGGAGTGA ATTCTCTCTT CATTGATGGC
TCCTTCCAGC TGCTCTACTT CATCTACTCT GTCCTGGTGA TCGTCTCAGC AGCCCTCTAC
CTGGCAGGGA TCGAGGCCTA CCTGGCCGTG ATGGTCTTTG CCCTGGTCCT GGGCTGGATG
AATGCCCTTT ACTTCACCCG TGGGCTGAAG CTGACGGGGA CCTATAGCAT CATGATCCAG
AAGATTCTCT TCAAGGACCT TTTCCGATTC CTGCTCGTCT ACTTGCTCTT CATGATCGGC
TACGCTTCAG CCCTGGTCTC CCTCCTGAAC CCGTGTGCCA ACATGAAGGT GTGCAATGAG
GACCAGACCA ACTGCACAGT GCCCACTTAC CCCTCGTGCC GTGACAGCGA GACCTTCAGC
ACCTTCCTCC TGGACCTGTT TAAGCTGACC ATCGGCATGG GCGACCTGGA GATGCTGAGC
AGCACCAAGT ACCCCGTGGT CTTCATCATC CTGCTGGTGA CCTACATCAT CCTCACCTTT
GTGCTGCTCC TCAACATGCT CATTGCCCTC ATGGGCGAGA CAGTGGGCCA GGTCTCCAAG
GAGAGCAAGC ACATCTGGAA GCTGCAGTGG GCCACCACCA TCCTGGACAT TGAGCGCTCC
TTCCCCGTAT TCCTGAGGAA GGCCTTCCGC TCTGGGGAGA TGGTCACCGT GGGCAAGAGC
TCGGACGGCA CTCCTGACCG CAGGTGGTGC TTCAGGGTGG ATGAGGTGAA CTGGTCTCAC
TGGAACCAGA ACTTGGGCAT CATCAACGAG GACCCGGGCA AGAATGAGAC CTACCAGTAT
TATGGCTTCT CGCATACCGT GGGCCGCCTC CGCAGGGATC GCTGGTCCTC GGTGGTACCC
CGCGTGGTGG AACTGAACAA GAACTCGAAC CCGGACGAGG TGGTGGTGCC TCTGGACAGC
ATGGGGAACC CCCGCTGCGA TGGCCACCAG CAGGGTTACC CCCGCAAGTG GAGGACTGAT
GACGCCCCGC TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001170899.1
CDS32..2506
Misc Feature(1)737..1060(+)
Misc Feature(2)755..1069(+)
Misc Feature(3)1550..2044(+)
Exon (1)1..417
Gene:TRPV4
Gene Synonym:
Exon (2)418..590
Gene:TRPV4
Gene Synonym:
Exon (3)591..743
Gene:TRPV4
Gene Synonym:
Exon (4)744..1042
Gene:TRPV4
Gene Synonym:
Exon (5)1043..1222
Gene:TRPV4
Gene Synonym:
Exon (6)1223..1381
Gene:TRPV4
Gene Synonym:
Exon (7)1382..1474
Gene:TRPV4
Gene Synonym:
Exon (8)1475..1548
Gene:TRPV4
Gene Synonym:
Exon (9)1549..1714
Gene:TRPV4
Gene Synonym:
Exon (10)1715..1781
Gene:TRPV4
Gene Synonym:
Exon (11)1782..2098
Gene:TRPV4
Gene Synonym:
Exon (12)2099..2226
Gene:TRPV4
Gene Synonym:
Exon (13)2227..2348
Gene:TRPV4
Gene Synonym:
Exon (14)2349..3040
Gene:TRPV4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001177428
Organism Homo sapiens (human)
Definition Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001177428

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG TGGGGAGGCT TTTCCTCTCT CCTCCCTGGC CAATCTGTTT
GAGGGGGAGG ATGGCTCCCT TTCGCCCTCA CCGGCTGATG CCAGTCGCCC TGCTGGCCCA
GGCGATGGGC GACCAAATCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC
AACCCCATCG ATCTGCTGGA GTCCACCCTA TATGAGTCCT CGGTGGTGCC TGGGCCCAAG
AAAGCACCCA TGGACTCACT GTTTGACTAC GGCACCTATC GTCACCACTC CAGTGACAAC
AAGAGGTGGA GGAAGAAGAT CATAGAGAAG CAGCCGCAGA GCCCCAAAGC CCCTGCCCCT
CAGCCGCCCC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG
GGCTCCACTG CTGACCTGGA CGGGCTGCTC CCATTCTTGC TGACCCACAA GAAACGCCTA
ACTGATGAGG AGTTTCGAGA GCCATCTACG GGGAAGACCT GCCTGCCCAA GGCCTTGCTG
AACCTGAGCA ATGGCCGCAA CGACACCATC CCTGTGCTGC TGGACATCGC GGAGCGCACC
GGCAACATGA GGGAGTTCAT TAACTCGCCC TTCCGTGACA TCTACTATCG AGGGGAGCTG
CCCCTGTCGC TGGCTGCCTG CACCAACCAG CCCCACATTG TCAACTACCT GACGGAGAAC
CCCCACAAGA AGGCGGACAT GCGGCGCCAG GACTCGCGAG GCAACACAGT GCTGCATGCG
CTGGTGGCCA TTGCTGACAA CACCCGTGAG AACACCAAGT TTGTTACCAA GATGTACGAC
CTGCTGCTGC TCAAGTGTGC CCGCCTCTTC CCCGACAGCA ACCTGGAGGC CGTGCTCAAC
AACGACGGCC TCTCGCCCCT CATGATGGCT GCCAAGACGG GCAAGATTGG GATCTTTCAG
CACATCATCC GGCGGGAGGT GACGGATGAG GACACACGGC ACCTGTCCCG CAAGTTCAAG
GACTGGGCCT ATGGGCCAGT GTATTCCTCG CTTTATGACC TCTCCTCCCT GGACACGTGT
GGGGAAGAGG CCTCCGTGCT GGAGATCCTG GTGTACAACA GCAAGATTGA GAACCGCCAC
GAGATGCTGG CTGTGGAGCC CATCAATGAA CTGCTGCGGG ACAAGTGGCG CAAGTTCGGG
GCCGTCTCCT TCTACATCAA CGTGGTCTCC TACCTGTGTG CCATGGTCAT CTTCACTCTC
ACCGCCTACT ACCAGCCGCT GGAGGGCACA CCGCCGTACC CTTACCGCAC CACGGTGGAC
TACCTGCGGC TGGCTGGCGA GGTCATTACG CTCTTCACTG GGGTCCTGTT CTTCTTCACC
AACATCAAAG ACTTGTTCAT GAAGAAATGC CCTGGAGTGA ATTCTCTCTT CATTGATGGC
TCCTTCCAGC TGCTCTACTT CATCTACTCT GTCCTGGTGA TCGTCTCAGC AGCCCTCTAC
CTGGCAGGGA TCGAGGCCTA CCTGGCCGTG ATGGTCTTTG CCCTGGTCCT GGGCTGGATG
AATGCCCTTT ACTTCACCCG TGGGCTGAAG CTGACGGGGA CCTATAGCAT CATGATCCAG
AAGATTCTCT TCAAGGACCT TTTCCGATTC CTGCTCGTCT ACTTGCTCTT CATGATCGGC
TACGCTTCAG CCCTGGTCTC CCTCCTGAAC CCGTGTGCCA ACATGAAGGT GTGCAATGAG
GACCAGACCA ACTGCACAGT GCCCACTTAC CCCTCGTGCC GTGACAGCGA GACCTTCAGC
ACCTTCCTCC TGGACCTGTT TAAGCTGACC ATCGGCATGG GCGACCTGGA GATGCTGAGC
AGCACCAAGT ACCCCGTGGT CTTCATCATC CTGCTGGTGA CCTACATCAT CCTCACCTTT
GTGCTGCTCC TCAACATGCT CATTGCCCTC ATGGGCGAGA CAGTGGGCCA GGTCTCCAAG
GAGAGCAAGC ACATCTGGAA GCTGCAGTGG GCCACCACCA TCCTGGACAT TGAGCGCTCC
TTCCCCGTAT TCCTGAGGAA GGCCTTCCGC TCTGGGGAGA TGGTCACCGT GGGCAAGAGC
TCGGACGGCA CTCCTGACCG CAGGTGGTGC TTCAGGGTGG ATGAGGTGAA CTGGTCTCAC
TGGAACCAGA ACTTGGGCAT CATCAACGAG GACCCGGGCA AGAATGAGAC CTACCAGTAT
TATGGCTTCT CGCATACCGT GGGCCGCCTC CGCAGGGATC GCTGGTCCTC GGTGGTACCC
CGCGTGGTGG AACTGAACAA GAACTCGAAC CCGGACGAGG TGGTGGTGCC TCTGGACAGC
ATGGGGAACC CCCGCTGCGA TGGCCACCAG CAGGGTTACC CCCGCAAGTG GAGGACTGAT
GACGCCCCGC TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25097
Clone ID Related Accession (Same CDS sequence) NM_001177431
Accession Version NM_001177431.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2514bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-MAR-2015
Organism Homo sapiens (human)
Product transient receptor potential cation channel subfamily V member 4 isoform d
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ059645.1, AC007834.40, AF279673.1 and AB032427.1. Transcript Variant: This variant (4) lacks a segment in the 5' coding region coding region, compared to variant 1. The encoded isoform (d) is shorter, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ059645.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG CGATGGGCGA CCAAATCTGC GCATGAAGTT CCAGGGCGCC
TTCCGCAAGG GGGTGCCCAA CCCCATCGAT CTGCTGGAGT CCACCCTATA TGAGTCCTCG
GTGGTGCCTG GGCCCAAGAA AGCACCCATG GACTCACTGT TTGACTACGG CACCTATCGT
CACCACTCCA GTGACAACAA GAGGTGGAGG AAGAAGATCA TAGAGAAGCA GCCGCAGAGC
CCCAAAGCCC CTGCCCCTCA GCCGCCCCCC ATCCTCAAAG TCTTCAACCG GCCTATCCTC
TTTGACATCG TGTCCCGGGG CTCCACTGCT GACCTGGACG GGCTGCTCCC ATTCTTGCTG
ACCCACAAGA AACGCCTAAC TGATGAGGAG TTTCGAGAGC CATCTACGGG GAAGACCTGC
CTGCCCAAGG CCTTGCTGAA CCTGAGCAAT GGCCGCAACG ACACCATCCC TGTGCTGCTG
GACATCGCGG AGCGCACCGG CAACATGAGG GAGTTCATTA ACTCGCCCTT CCGTGACATC
TACTATCGAG GTCAGACAGC CCTGCACATC GCCATTGAGC GTCGCTGCAA ACACTACGTG
GAACTTCTCG TGGCCCAGGG AGCTGATGTC CACGCCCAGG CCCGTGGGCG CTTCTTCCAG
CCCAAGGATG AGGGGGGCTA CTTCTACTTT GGGGAGCTGC CCCTGTCGCT GGCTGCCTGC
ACCAACCAGC CCCACATTGT CAACTACCTG ACGGAGAACC CCCACAAGAA GGCGGACATG
CGGCGCCAGG ACTCGCGAGG CAACACAGTG CTGCATGCGC TGGTGGCCAT TGCTGACAAC
ACCCGTGAGA ACACCAAGTT TGTTACCAAG ATGTACGACC TGCTGCTGCT CAAGTGTGCC
CGCCTCTTCC CCGACAGCAA CCTGGAGGCC GTGCTCAACA ACGACGGCCT CTCGCCCCTC
ATGATGGCTG CCAAGACGGG CAAGATTGGG ATCTTTCAGC ACATCATCCG GCGGGAGGTG
ACGGATGAGG ACACACGGCA CCTGTCCCGC AAGTTCAAGG ACTGGGCCTA TGGGCCAGTG
TATTCCTCGC TTTATGACCT CTCCTCCCTG GACACGTGTG GGGAAGAGGC CTCCGTGCTG
GAGATCCTGG TGTACAACAG CAAGATTGAG AACCGCCACG AGATGCTGGC TGTGGAGCCC
ATCAATGAAC TGCTGCGGGA CAAGTGGCGC AAGTTCGGGG CCGTCTCCTT CTACATCAAC
GTGGTCTCCT ACCTGTGTGC CATGGTCATC TTCACTCTCA CCGCCTACTA CCAGCCGCTG
GAGGGCACAC CGCCGTACCC TTACCGCACC ACGGTGGACT ACCTGCGGCT GGCTGGCGAG
GTCATTACGC TCTTCACTGG GGTCCTGTTC TTCTTCACCA ACATCAAAGA CTTGTTCATG
AAGAAATGCC CTGGAGTGAA TTCTCTCTTC ATTGATGGCT CCTTCCAGCT GCTCTACTTC
ATCTACTCTG TCCTGGTGAT CGTCTCAGCA GCCCTCTACC TGGCAGGGAT CGAGGCCTAC
CTGGCCGTGA TGGTCTTTGC CCTGGTCCTG GGCTGGATGA ATGCCCTTTA CTTCACCCGT
GGGCTGAAGC TGACGGGGAC CTATAGCATC ATGATCCAGA AGATTCTCTT CAAGGACCTT
TTCCGATTCC TGCTCGTCTA CTTGCTCTTC ATGATCGGCT ACGCTTCAGC CCTGGTCTCC
CTCCTGAACC CGTGTGCCAA CATGAAGGTG TGCAATGAGG ACCAGACCAA CTGCACAGTG
CCCACTTACC CCTCGTGCCG TGACAGCGAG ACCTTCAGCA CCTTCCTCCT GGACCTGTTT
AAGCTGACCA TCGGCATGGG CGACCTGGAG ATGCTGAGCA GCACCAAGTA CCCCGTGGTC
TTCATCATCC TGCTGGTGAC CTACATCATC CTCACCTTTG TGCTGCTCCT CAACATGCTC
ATTGCCCTCA TGGGCGAGAC AGTGGGCCAG GTCTCCAAGG AGAGCAAGCA CATCTGGAAG
CTGCAGTGGG CCACCACCAT CCTGGACATT GAGCGCTCCT TCCCCGTATT CCTGAGGAAG
GCCTTCCGCT CTGGGGAGAT GGTCACCGTG GGCAAGAGCT CGGACGGCAC TCCTGACCGC
AGGTGGTGCT TCAGGGTGGA TGAGGTGAAC TGGTCTCACT GGAACCAGAA CTTGGGCATC
ATCAACGAGG ACCCGGGCAA GAATGAGACC TACCAGTATT ATGGCTTCTC GCATACCGTG
GGCCGCCTCC GCAGGGATCG CTGGTCCTCG GTGGTACCCC GCGTGGTGGA ACTGAACAAG
AACTCGAACC CGGACGAGGT GGTGGTGCCT CTGGACAGCA TGGGGAACCC CCGCTGCGAT
GGCCACCAGC AGGGTTACCC CCGCAAGTGG AGGACTGATG ACGCCCCGCT CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001170902.1
CDS1..2514
Misc Feature(1)538..852(+)
Misc Feature(2)610..1068(+)
Misc Feature(3)1558..2052(+)
Exon (1)1..79
Gene:TRPV4
Gene Synonym:
Exon (2)80..284
Gene:TRPV4
Gene Synonym:
Exon (3)285..457
Gene:TRPV4
Gene Synonym:
Exon (4)458..610
Gene:TRPV4
Gene Synonym:
Exon (5)611..751
Gene:TRPV4
Gene Synonym:
Exon (6)752..1050
Gene:TRPV4
Gene Synonym:
Exon (7)1051..1230
Gene:TRPV4
Gene Synonym:
Exon (8)1231..1389
Gene:TRPV4
Gene Synonym:
Exon (9)1390..1482
Gene:TRPV4
Gene Synonym:
Exon (10)1483..1556
Gene:TRPV4
Gene Synonym:
Exon (11)1557..1722
Gene:TRPV4
Gene Synonym:
Exon (12)1723..1789
Gene:TRPV4
Gene Synonym:
Exon (13)1790..2106
Gene:TRPV4
Gene Synonym:
Exon (14)2107..2234
Gene:TRPV4
Gene Synonym:
Exon (15)2235..2356
Gene:TRPV4
Gene Synonym:
Exon (16)2357..3048
Gene:TRPV4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001177431
Organism Homo sapiens (human)
Definition Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001177431

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG CGATGGGCGA CCAAATCTGC GCATGAAGTT CCAGGGCGCC
TTCCGCAAGG GGGTGCCCAA CCCCATCGAT CTGCTGGAGT CCACCCTATA TGAGTCCTCG
GTGGTGCCTG GGCCCAAGAA AGCACCCATG GACTCACTGT TTGACTACGG CACCTATCGT
CACCACTCCA GTGACAACAA GAGGTGGAGG AAGAAGATCA TAGAGAAGCA GCCGCAGAGC
CCCAAAGCCC CTGCCCCTCA GCCGCCCCCC ATCCTCAAAG TCTTCAACCG GCCTATCCTC
TTTGACATCG TGTCCCGGGG CTCCACTGCT GACCTGGACG GGCTGCTCCC ATTCTTGCTG
ACCCACAAGA AACGCCTAAC TGATGAGGAG TTTCGAGAGC CATCTACGGG GAAGACCTGC
CTGCCCAAGG CCTTGCTGAA CCTGAGCAAT GGCCGCAACG ACACCATCCC TGTGCTGCTG
GACATCGCGG AGCGCACCGG CAACATGAGG GAGTTCATTA ACTCGCCCTT CCGTGACATC
TACTATCGAG GTCAGACAGC CCTGCACATC GCCATTGAGC GTCGCTGCAA ACACTACGTG
GAACTTCTCG TGGCCCAGGG AGCTGATGTC CACGCCCAGG CCCGTGGGCG CTTCTTCCAG
CCCAAGGATG AGGGGGGCTA CTTCTACTTT GGGGAGCTGC CCCTGTCGCT GGCTGCCTGC
ACCAACCAGC CCCACATTGT CAACTACCTG ACGGAGAACC CCCACAAGAA GGCGGACATG
CGGCGCCAGG ACTCGCGAGG CAACACAGTG CTGCATGCGC TGGTGGCCAT TGCTGACAAC
ACCCGTGAGA ACACCAAGTT TGTTACCAAG ATGTACGACC TGCTGCTGCT CAAGTGTGCC
CGCCTCTTCC CCGACAGCAA CCTGGAGGCC GTGCTCAACA ACGACGGCCT CTCGCCCCTC
ATGATGGCTG CCAAGACGGG CAAGATTGGG ATCTTTCAGC ACATCATCCG GCGGGAGGTG
ACGGATGAGG ACACACGGCA CCTGTCCCGC AAGTTCAAGG ACTGGGCCTA TGGGCCAGTG
TATTCCTCGC TTTATGACCT CTCCTCCCTG GACACGTGTG GGGAAGAGGC CTCCGTGCTG
GAGATCCTGG TGTACAACAG CAAGATTGAG AACCGCCACG AGATGCTGGC TGTGGAGCCC
ATCAATGAAC TGCTGCGGGA CAAGTGGCGC AAGTTCGGGG CCGTCTCCTT CTACATCAAC
GTGGTCTCCT ACCTGTGTGC CATGGTCATC TTCACTCTCA CCGCCTACTA CCAGCCGCTG
GAGGGCACAC CGCCGTACCC TTACCGCACC ACGGTGGACT ACCTGCGGCT GGCTGGCGAG
GTCATTACGC TCTTCACTGG GGTCCTGTTC TTCTTCACCA ACATCAAAGA CTTGTTCATG
AAGAAATGCC CTGGAGTGAA TTCTCTCTTC ATTGATGGCT CCTTCCAGCT GCTCTACTTC
ATCTACTCTG TCCTGGTGAT CGTCTCAGCA GCCCTCTACC TGGCAGGGAT CGAGGCCTAC
CTGGCCGTGA TGGTCTTTGC CCTGGTCCTG GGCTGGATGA ATGCCCTTTA CTTCACCCGT
GGGCTGAAGC TGACGGGGAC CTATAGCATC ATGATCCAGA AGATTCTCTT CAAGGACCTT
TTCCGATTCC TGCTCGTCTA CTTGCTCTTC ATGATCGGCT ACGCTTCAGC CCTGGTCTCC
CTCCTGAACC CGTGTGCCAA CATGAAGGTG TGCAATGAGG ACCAGACCAA CTGCACAGTG
CCCACTTACC CCTCGTGCCG TGACAGCGAG ACCTTCAGCA CCTTCCTCCT GGACCTGTTT
AAGCTGACCA TCGGCATGGG CGACCTGGAG ATGCTGAGCA GCACCAAGTA CCCCGTGGTC
TTCATCATCC TGCTGGTGAC CTACATCATC CTCACCTTTG TGCTGCTCCT CAACATGCTC
ATTGCCCTCA TGGGCGAGAC AGTGGGCCAG GTCTCCAAGG AGAGCAAGCA CATCTGGAAG
CTGCAGTGGG CCACCACCAT CCTGGACATT GAGCGCTCCT TCCCCGTATT CCTGAGGAAG
GCCTTCCGCT CTGGGGAGAT GGTCACCGTG GGCAAGAGCT CGGACGGCAC TCCTGACCGC
AGGTGGTGCT TCAGGGTGGA TGAGGTGAAC TGGTCTCACT GGAACCAGAA CTTGGGCATC
ATCAACGAGG ACCCGGGCAA GAATGAGACC TACCAGTATT ATGGCTTCTC GCATACCGTG
GGCCGCCTCC GCAGGGATCG CTGGTCCTCG GTGGTACCCC GCGTGGTGGA ACTGAACAAG
AACTCGAACC CGGACGAGGT GGTGGTGCCT CTGGACAGCA TGGGGAACCC CCGCTGCGAT
GGCCACCAGC AGGGTTACCC CCGCAAGTGG AGGACTGATG ACGCCCCGCT CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27266
Clone ID Related Accession (Same CDS sequence) NM_001177433 , XM_011538633
Accession Version NM_001177433.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2295bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-MAR-2015
Organism Homo sapiens (human)
Product transient receptor potential cation channel subfamily V member 4 isoform c
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC143315.1, DQ059646.1 and AB032427.1. Transcript Variant: This variant (3) lacks two in-frame exons in the coding region, compared to variant 1. The encoded isoform (c) is shorter, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ059646.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG TGGGGAGGCT TTTCCTCTCT CCTCCCTGGC CAATCTGTTT
GAGGGGGAGG ATGGCTCCCT TTCGCCCTCA CCGGCTGATG CCAGTCGCCC TGCTGGCCCA
GGCGATGGGC GACCAAATCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC
AACCCCATCG ATCTGCTGGA GTCCACCCTA TATGAGTCCT CGGTGGTGCC TGGGCCCAAG
AAAGCACCCA TGGACTCACT GTTTGACTAC GGCACCTATC GTCACCACTC CAGTGACAAC
AAGAGGTGGA GGAAGAAGAT CATAGAGAAG CAGCCGCAGA GCCCCAAAGC CCCTGCCCCT
CAGCCGCCCC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG
GGCTCCACTG CTGACCTGGA CGGGCTGCTC CCATTCTTGC TGACCCACAA GAAACGCCTA
ACTGATGAGG AGTTTCGAGA GCCATCTACG GGGAAGACCT GCCTGCCCAA GGCCTTGCTG
AACCTGAGCA ATGGCCGCAA CGACACCATC CCTGTGCTGC TGGACATCGC GGAGCGCACC
GGCAACATGA GGGAGTTCAT TAACTCGCCC TTCCGTGACA TCTACTATCG AGGGGAGCTG
CCCCTGTCGC TGGCTGCCTG CACCAACCAG CCCCACATTG TCAACTACCT GACGGAGAAC
CCCCACAAGA AGGCGGACAT GCGGCGCCAG GACTCGCGAG GCAACACAGT GCTGCATGCG
CTGGTGGCCA TTGCTGACAA CACCCGTGAG AACACCAAGT TTGTTACCAA GATGTACGAC
CTGCTGCTGC TCAAGTGTGC CCGCCTCTTC CCCGACAGCA ACCTGGAGGC CGTGCTCAAC
AACGACGGCC TCTCGCCCCT CATGATGGCT GCCAAGACGG GCAAGATTGG GAACCGCCAC
GAGATGCTGG CTGTGGAGCC CATCAATGAA CTGCTGCGGG ACAAGTGGCG CAAGTTCGGG
GCCGTCTCCT TCTACATCAA CGTGGTCTCC TACCTGTGTG CCATGGTCAT CTTCACTCTC
ACCGCCTACT ACCAGCCGCT GGAGGGCACA CCGCCGTACC CTTACCGCAC CACGGTGGAC
TACCTGCGGC TGGCTGGCGA GGTCATTACG CTCTTCACTG GGGTCCTGTT CTTCTTCACC
AACATCAAAG ACTTGTTCAT GAAGAAATGC CCTGGAGTGA ATTCTCTCTT CATTGATGGC
TCCTTCCAGC TGCTCTACTT CATCTACTCT GTCCTGGTGA TCGTCTCAGC AGCCCTCTAC
CTGGCAGGGA TCGAGGCCTA CCTGGCCGTG ATGGTCTTTG CCCTGGTCCT GGGCTGGATG
AATGCCCTTT ACTTCACCCG TGGGCTGAAG CTGACGGGGA CCTATAGCAT CATGATCCAG
AAGATTCTCT TCAAGGACCT TTTCCGATTC CTGCTCGTCT ACTTGCTCTT CATGATCGGC
TACGCTTCAG CCCTGGTCTC CCTCCTGAAC CCGTGTGCCA ACATGAAGGT GTGCAATGAG
GACCAGACCA ACTGCACAGT GCCCACTTAC CCCTCGTGCC GTGACAGCGA GACCTTCAGC
ACCTTCCTCC TGGACCTGTT TAAGCTGACC ATCGGCATGG GCGACCTGGA GATGCTGAGC
AGCACCAAGT ACCCCGTGGT CTTCATCATC CTGCTGGTGA CCTACATCAT CCTCACCTTT
GTGCTGCTCC TCAACATGCT CATTGCCCTC ATGGGCGAGA CAGTGGGCCA GGTCTCCAAG
GAGAGCAAGC ACATCTGGAA GCTGCAGTGG GCCACCACCA TCCTGGACAT TGAGCGCTCC
TTCCCCGTAT TCCTGAGGAA GGCCTTCCGC TCTGGGGAGA TGGTCACCGT GGGCAAGAGC
TCGGACGGCA CTCCTGACCG CAGGTGGTGC TTCAGGGTGG ATGAGGTGAA CTGGTCTCAC
TGGAACCAGA ACTTGGGCAT CATCAACGAG GACCCGGGCA AGAATGAGAC CTACCAGTAT
TATGGCTTCT CGCATACCGT GGGCCGCCTC CGCAGGGATC GCTGGTCCTC GGTGGTACCC
CGCGTGGTGG AACTGAACAA GAACTCGAAC CCGGACGAGG TGGTGGTGCC TCTGGACAGC
ATGGGGAACC CCCGCTGCGA TGGCCACCAG CAGGGTTACC CCCGCAAGTG GAGGACTGAT
GACGCCCCGC TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001170904.1
CDS32..2326
Misc Feature(1)476..2107(+)
Misc Feature(2)737..1039(+)
Exon (1)1..417
Gene:TRPV4
Gene Synonym:
Exon (2)418..590
Gene:TRPV4
Gene Synonym:
Exon (3)591..743
Gene:TRPV4
Gene Synonym:
Exon (4)744..1042
Gene:TRPV4
Gene Synonym:
Exon (5)1043..1201
Gene:TRPV4
Gene Synonym:
Exon (6)1202..1294
Gene:TRPV4
Gene Synonym:
Exon (7)1295..1368
Gene:TRPV4
Gene Synonym:
Exon (8)1369..1534
Gene:TRPV4
Gene Synonym:
Exon (9)1535..1601
Gene:TRPV4
Gene Synonym:
Exon (10)1602..1918
Gene:TRPV4
Gene Synonym:
Exon (11)1919..2046
Gene:TRPV4
Gene Synonym:
Exon (12)2047..2168
Gene:TRPV4
Gene Synonym:
Exon (13)2169..2860
Gene:TRPV4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001177433
Organism Homo sapiens (human)
Definition Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001177433

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG TGGGGAGGCT TTTCCTCTCT CCTCCCTGGC CAATCTGTTT
GAGGGGGAGG ATGGCTCCCT TTCGCCCTCA CCGGCTGATG CCAGTCGCCC TGCTGGCCCA
GGCGATGGGC GACCAAATCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC
AACCCCATCG ATCTGCTGGA GTCCACCCTA TATGAGTCCT CGGTGGTGCC TGGGCCCAAG
AAAGCACCCA TGGACTCACT GTTTGACTAC GGCACCTATC GTCACCACTC CAGTGACAAC
AAGAGGTGGA GGAAGAAGAT CATAGAGAAG CAGCCGCAGA GCCCCAAAGC CCCTGCCCCT
CAGCCGCCCC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG
GGCTCCACTG CTGACCTGGA CGGGCTGCTC CCATTCTTGC TGACCCACAA GAAACGCCTA
ACTGATGAGG AGTTTCGAGA GCCATCTACG GGGAAGACCT GCCTGCCCAA GGCCTTGCTG
AACCTGAGCA ATGGCCGCAA CGACACCATC CCTGTGCTGC TGGACATCGC GGAGCGCACC
GGCAACATGA GGGAGTTCAT TAACTCGCCC TTCCGTGACA TCTACTATCG AGGGGAGCTG
CCCCTGTCGC TGGCTGCCTG CACCAACCAG CCCCACATTG TCAACTACCT GACGGAGAAC
CCCCACAAGA AGGCGGACAT GCGGCGCCAG GACTCGCGAG GCAACACAGT GCTGCATGCG
CTGGTGGCCA TTGCTGACAA CACCCGTGAG AACACCAAGT TTGTTACCAA GATGTACGAC
CTGCTGCTGC TCAAGTGTGC CCGCCTCTTC CCCGACAGCA ACCTGGAGGC CGTGCTCAAC
AACGACGGCC TCTCGCCCCT CATGATGGCT GCCAAGACGG GCAAGATTGG GAACCGCCAC
GAGATGCTGG CTGTGGAGCC CATCAATGAA CTGCTGCGGG ACAAGTGGCG CAAGTTCGGG
GCCGTCTCCT TCTACATCAA CGTGGTCTCC TACCTGTGTG CCATGGTCAT CTTCACTCTC
ACCGCCTACT ACCAGCCGCT GGAGGGCACA CCGCCGTACC CTTACCGCAC CACGGTGGAC
TACCTGCGGC TGGCTGGCGA GGTCATTACG CTCTTCACTG GGGTCCTGTT CTTCTTCACC
AACATCAAAG ACTTGTTCAT GAAGAAATGC CCTGGAGTGA ATTCTCTCTT CATTGATGGC
TCCTTCCAGC TGCTCTACTT CATCTACTCT GTCCTGGTGA TCGTCTCAGC AGCCCTCTAC
CTGGCAGGGA TCGAGGCCTA CCTGGCCGTG ATGGTCTTTG CCCTGGTCCT GGGCTGGATG
AATGCCCTTT ACTTCACCCG TGGGCTGAAG CTGACGGGGA CCTATAGCAT CATGATCCAG
AAGATTCTCT TCAAGGACCT TTTCCGATTC CTGCTCGTCT ACTTGCTCTT CATGATCGGC
TACGCTTCAG CCCTGGTCTC CCTCCTGAAC CCGTGTGCCA ACATGAAGGT GTGCAATGAG
GACCAGACCA ACTGCACAGT GCCCACTTAC CCCTCGTGCC GTGACAGCGA GACCTTCAGC
ACCTTCCTCC TGGACCTGTT TAAGCTGACC ATCGGCATGG GCGACCTGGA GATGCTGAGC
AGCACCAAGT ACCCCGTGGT CTTCATCATC CTGCTGGTGA CCTACATCAT CCTCACCTTT
GTGCTGCTCC TCAACATGCT CATTGCCCTC ATGGGCGAGA CAGTGGGCCA GGTCTCCAAG
GAGAGCAAGC ACATCTGGAA GCTGCAGTGG GCCACCACCA TCCTGGACAT TGAGCGCTCC
TTCCCCGTAT TCCTGAGGAA GGCCTTCCGC TCTGGGGAGA TGGTCACCGT GGGCAAGAGC
TCGGACGGCA CTCCTGACCG CAGGTGGTGC TTCAGGGTGG ATGAGGTGAA CTGGTCTCAC
TGGAACCAGA ACTTGGGCAT CATCAACGAG GACCCGGGCA AGAATGAGAC CTACCAGTAT
TATGGCTTCT CGCATACCGT GGGCCGCCTC CGCAGGGATC GCTGGTCCTC GGTGGTACCC
CGCGTGGTGG AACTGAACAA GAACTCGAAC CCGGACGAGG TGGTGGTGCC TCTGGACAGC
ATGGGGAACC CCCGCTGCGA TGGCCACCAG CAGGGTTACC CCCGCAAGTG GAGGACTGAT
GACGCCCCGC TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21707
Clone ID Related Accession (Same CDS sequence) NM_021625 , XM_005253918 , XM_011538630
Accession Version NM_021625.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2616bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-MAR-2015
Organism Homo sapiens (human)
Product transient receptor potential cation channel subfamily V member 4 isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ296305.1, AC007834.40 and AB032427.1. This sequence is a reference standard in the RefSeqGene project. On Apr 9, 2010 this sequence version replaced gi:22547183. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ296305.1, AL834192.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG TGGGGAGGCT TTTCCTCTCT CCTCCCTGGC CAATCTGTTT
GAGGGGGAGG ATGGCTCCCT TTCGCCCTCA CCGGCTGATG CCAGTCGCCC TGCTGGCCCA
GGCGATGGGC GACCAAATCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC
AACCCCATCG ATCTGCTGGA GTCCACCCTA TATGAGTCCT CGGTGGTGCC TGGGCCCAAG
AAAGCACCCA TGGACTCACT GTTTGACTAC GGCACCTATC GTCACCACTC CAGTGACAAC
AAGAGGTGGA GGAAGAAGAT CATAGAGAAG CAGCCGCAGA GCCCCAAAGC CCCTGCCCCT
CAGCCGCCCC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG
GGCTCCACTG CTGACCTGGA CGGGCTGCTC CCATTCTTGC TGACCCACAA GAAACGCCTA
ACTGATGAGG AGTTTCGAGA GCCATCTACG GGGAAGACCT GCCTGCCCAA GGCCTTGCTG
AACCTGAGCA ATGGCCGCAA CGACACCATC CCTGTGCTGC TGGACATCGC GGAGCGCACC
GGCAACATGA GGGAGTTCAT TAACTCGCCC TTCCGTGACA TCTACTATCG AGGTCAGACA
GCCCTGCACA TCGCCATTGA GCGTCGCTGC AAACACTACG TGGAACTTCT CGTGGCCCAG
GGAGCTGATG TCCACGCCCA GGCCCGTGGG CGCTTCTTCC AGCCCAAGGA TGAGGGGGGC
TACTTCTACT TTGGGGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATT
GTCAACTACC TGACGGAGAA CCCCCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGA
GGCAACACAG TGCTGCATGC GCTGGTGGCC ATTGCTGACA ACACCCGTGA GAACACCAAG
TTTGTTACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC
AACCTGGAGG CCGTGCTCAA CAACGACGGC CTCTCGCCCC TCATGATGGC TGCCAAGACG
GGCAAGATTG GGATCTTTCA GCACATCATC CGGCGGGAGG TGACGGATGA GGACACACGG
CACCTGTCCC GCAAGTTCAA GGACTGGGCC TATGGGCCAG TGTATTCCTC GCTTTATGAC
CTCTCCTCCC TGGACACGTG TGGGGAAGAG GCCTCCGTGC TGGAGATCCT GGTGTACAAC
AGCAAGATTG AGAACCGCCA CGAGATGCTG GCTGTGGAGC CCATCAATGA ACTGCTGCGG
GACAAGTGGC GCAAGTTCGG GGCCGTCTCC TTCTACATCA ACGTGGTCTC CTACCTGTGT
GCCATGGTCA TCTTCACTCT CACCGCCTAC TACCAGCCGC TGGAGGGCAC ACCGCCGTAC
CCTTACCGCA CCACGGTGGA CTACCTGCGG CTGGCTGGCG AGGTCATTAC GCTCTTCACT
GGGGTCCTGT TCTTCTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG
AATTCTCTCT TCATTGATGG CTCCTTCCAG CTGCTCTACT TCATCTACTC TGTCCTGGTG
ATCGTCTCAG CAGCCCTCTA CCTGGCAGGG ATCGAGGCCT ACCTGGCCGT GATGGTCTTT
GCCCTGGTCC TGGGCTGGAT GAATGCCCTT TACTTCACCC GTGGGCTGAA GCTGACGGGG
ACCTATAGCA TCATGATCCA GAAGATTCTC TTCAAGGACC TTTTCCGATT CCTGCTCGTC
TACTTGCTCT TCATGATCGG CTACGCTTCA GCCCTGGTCT CCCTCCTGAA CCCGTGTGCC
AACATGAAGG TGTGCAATGA GGACCAGACC AACTGCACAG TGCCCACTTA CCCCTCGTGC
CGTGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTGT TTAAGCTGAC CATCGGCATG
GGCGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTG
ACCTACATCA TCCTCACCTT TGTGCTGCTC CTCAACATGC TCATTGCCCT CATGGGCGAG
ACAGTGGGCC AGGTCTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC
ATCCTGGACA TTGAGCGCTC CTTCCCCGTA TTCCTGAGGA AGGCCTTCCG CTCTGGGGAG
ATGGTCACCG TGGGCAAGAG CTCGGACGGC ACTCCTGACC GCAGGTGGTG CTTCAGGGTG
GATGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATCAACGA GGACCCGGGC
AAGAATGAGA CCTACCAGTA TTATGGCTTC TCGCATACCG TGGGCCGCCT CCGCAGGGAT
CGCTGGTCCT CGGTGGTACC CCGCGTGGTG GAACTGAACA AGAACTCGAA CCCGGACGAG
GTGGTGGTGC CTCTGGACAG CATGGGGAAC CCCCGCTGCG ATGGCCACCA GCAGGGTTAC
CCCCGCAAGT GGAGGACTGA TGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_067638.3
CDS90..2705
Misc Feature(1)729..1043(+)
Misc Feature(2)798..887(+)
Misc Feature(3)801..1259(+)
Misc Feature(4)846..848(+)
Misc Feature(5)939..1028(+)
Misc Feature(6)1194..1283(+)
Misc Feature(7)1320..1322(+)
Misc Feature(8)1485..1547(+)
Misc Feature(9)1614..1676(+)
Misc Feature(10)1740..1802(+)
Misc Feature(11)1749..2243(+)
Misc Feature(12)1806..1868(+)
Misc Feature(13)1938..2000(+)
Misc Feature(14)2160..2222(+)
Misc Feature(15)2523..2582(+)
Misc Feature(16)2559..2561(+)
Exon (1)1..58
Gene:TRPV4
Gene Synonym:
Exon (2)59..475
Gene:TRPV4
Gene Synonym:
Exon (3)476..648
Gene:TRPV4
Gene Synonym:
Exon (4)649..801
Gene:TRPV4
Gene Synonym:
Exon (5)802..942
Gene:TRPV4
Gene Synonym:
Exon (6)943..1241
Gene:TRPV4
Gene Synonym:
Exon (7)1242..1421
Gene:TRPV4
Gene Synonym:
Exon (8)1422..1580
Gene:TRPV4
Gene Synonym:
Exon (9)1581..1673
Gene:TRPV4
Gene Synonym:
Exon (10)1674..1747
Gene:TRPV4
Gene Synonym:
Exon (11)1748..1913
Gene:TRPV4
Gene Synonym:
Exon (12)1914..1980
Gene:TRPV4
Gene Synonym:
Exon (13)1981..2297
Gene:TRPV4
Gene Synonym:
Exon (14)2298..2425
Gene:TRPV4
Gene Synonym:
Exon (15)2426..2547
Gene:TRPV4
Gene Synonym:
Exon (16)2548..3239
Gene:TRPV4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_021625
Organism Homo sapiens (human)
Definition Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_021625

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG TGGGGAGGCT TTTCCTCTCT CCTCCCTGGC CAATCTGTTT
GAGGGGGAGG ATGGCTCCCT TTCGCCCTCA CCGGCTGATG CCAGTCGCCC TGCTGGCCCA
GGCGATGGGC GACCAAATCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC
AACCCCATCG ATCTGCTGGA GTCCACCCTA TATGAGTCCT CGGTGGTGCC TGGGCCCAAG
AAAGCACCCA TGGACTCACT GTTTGACTAC GGCACCTATC GTCACCACTC CAGTGACAAC
AAGAGGTGGA GGAAGAAGAT CATAGAGAAG CAGCCGCAGA GCCCCAAAGC CCCTGCCCCT
CAGCCGCCCC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG
GGCTCCACTG CTGACCTGGA CGGGCTGCTC CCATTCTTGC TGACCCACAA GAAACGCCTA
ACTGATGAGG AGTTTCGAGA GCCATCTACG GGGAAGACCT GCCTGCCCAA GGCCTTGCTG
AACCTGAGCA ATGGCCGCAA CGACACCATC CCTGTGCTGC TGGACATCGC GGAGCGCACC
GGCAACATGA GGGAGTTCAT TAACTCGCCC TTCCGTGACA TCTACTATCG AGGTCAGACA
GCCCTGCACA TCGCCATTGA GCGTCGCTGC AAACACTACG TGGAACTTCT CGTGGCCCAG
GGAGCTGATG TCCACGCCCA GGCCCGTGGG CGCTTCTTCC AGCCCAAGGA TGAGGGGGGC
TACTTCTACT TTGGGGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATT
GTCAACTACC TGACGGAGAA CCCCCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGA
GGCAACACAG TGCTGCATGC GCTGGTGGCC ATTGCTGACA ACACCCGTGA GAACACCAAG
TTTGTTACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC
AACCTGGAGG CCGTGCTCAA CAACGACGGC CTCTCGCCCC TCATGATGGC TGCCAAGACG
GGCAAGATTG GGATCTTTCA GCACATCATC CGGCGGGAGG TGACGGATGA GGACACACGG
CACCTGTCCC GCAAGTTCAA GGACTGGGCC TATGGGCCAG TGTATTCCTC GCTTTATGAC
CTCTCCTCCC TGGACACGTG TGGGGAAGAG GCCTCCGTGC TGGAGATCCT GGTGTACAAC
AGCAAGATTG AGAACCGCCA CGAGATGCTG GCTGTGGAGC CCATCAATGA ACTGCTGCGG
GACAAGTGGC GCAAGTTCGG GGCCGTCTCC TTCTACATCA ACGTGGTCTC CTACCTGTGT
GCCATGGTCA TCTTCACTCT CACCGCCTAC TACCAGCCGC TGGAGGGCAC ACCGCCGTAC
CCTTACCGCA CCACGGTGGA CTACCTGCGG CTGGCTGGCG AGGTCATTAC GCTCTTCACT
GGGGTCCTGT TCTTCTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG
AATTCTCTCT TCATTGATGG CTCCTTCCAG CTGCTCTACT TCATCTACTC TGTCCTGGTG
ATCGTCTCAG CAGCCCTCTA CCTGGCAGGG ATCGAGGCCT ACCTGGCCGT GATGGTCTTT
GCCCTGGTCC TGGGCTGGAT GAATGCCCTT TACTTCACCC GTGGGCTGAA GCTGACGGGG
ACCTATAGCA TCATGATCCA GAAGATTCTC TTCAAGGACC TTTTCCGATT CCTGCTCGTC
TACTTGCTCT TCATGATCGG CTACGCTTCA GCCCTGGTCT CCCTCCTGAA CCCGTGTGCC
AACATGAAGG TGTGCAATGA GGACCAGACC AACTGCACAG TGCCCACTTA CCCCTCGTGC
CGTGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTGT TTAAGCTGAC CATCGGCATG
GGCGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTG
ACCTACATCA TCCTCACCTT TGTGCTGCTC CTCAACATGC TCATTGCCCT CATGGGCGAG
ACAGTGGGCC AGGTCTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC
ATCCTGGACA TTGAGCGCTC CTTCCCCGTA TTCCTGAGGA AGGCCTTCCG CTCTGGGGAG
ATGGTCACCG TGGGCAAGAG CTCGGACGGC ACTCCTGACC GCAGGTGGTG CTTCAGGGTG
GATGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATCAACGA GGACCCGGGC
AAGAATGAGA CCTACCAGTA TTATGGCTTC TCGCATACCG TGGGCCGCCT CCGCAGGGAT
CGCTGGTCCT CGGTGGTACC CCGCGTGGTG GAACTGAACA AGAACTCGAA CCCGGACGAG
GTGGTGGTGC CTCTGGACAG CATGGGGAAC CCCCGCTGCG ATGGCCACCA GCAGGGTTAC
CCCCGCAAGT GGAGGACTGA TGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23056
Clone ID Related Accession (Same CDS sequence) NM_147204 , XM_011538632
Accession Version NM_147204.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2436bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-MAR-2015
Organism Homo sapiens (human)
Product transient receptor potential cation channel subfamily V member 4 isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC143315.1, AB073669.1, AC007834.40 and AB032427.1. On Apr 9, 2010 this sequence version replaced gi:22547179. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1, resulting in an isoform (b) that lacks an internal segment compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB073669.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG TGGGGAGGCT TTTCCTCTCT CCTCCCTGGC CAATCTGTTT
GAGGGGGAGG ATGGCTCCCT TTCGCCCTCA CCGGCTGATG CCAGTCGCCC TGCTGGCCCA
GGCGATGGGC GACCAAATCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC
AACCCCATCG ATCTGCTGGA GTCCACCCTA TATGAGTCCT CGGTGGTGCC TGGGCCCAAG
AAAGCACCCA TGGACTCACT GTTTGACTAC GGCACCTATC GTCACCACTC CAGTGACAAC
AAGAGGTGGA GGAAGAAGAT CATAGAGAAG CAGCCGCAGA GCCCCAAAGC CCCTGCCCCT
CAGCCGCCCC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG
GGCTCCACTG CTGACCTGGA CGGGCTGCTC CCATTCTTGC TGACCCACAA GAAACGCCTA
ACTGATGAGG AGTTTCGAGA GCCATCTACG GGGAAGACCT GCCTGCCCAA GGCCTTGCTG
AACCTGAGCA ATGGCCGCAA CGACACCATC CCTGTGCTGC TGGACATCGC GGAGCGCACC
GGCAACATGA GGGAGTTCAT TAACTCGCCC TTCCGTGACA TCTACTATCG AGGTCAGACA
GCCCTGCACA TCGCCATTGA GCGTCGCTGC AAACACTACG TGGAACTTCT CGTGGCCCAG
GGAGCTGATG TCCACGCCCA GGCCCGTGGG CGCTTCTTCC AGCCCAAGGA TGAGGGGGGC
TACTTCTACT TTGGGGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATT
GTCAACTACC TGACGGAGAA CCCCCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGA
GGCAACACAG TGCTGCATGC GCTGGTGGCC ATTGCTGACA ACACCCGTGA GAACACCAAG
TTTGTTACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC
AACCTGGAGG CCGTGCTCAA CAACGACGGC CTCTCGCCCC TCATGATGGC TGCCAAGACG
GGCAAGATTG GGAACCGCCA CGAGATGCTG GCTGTGGAGC CCATCAATGA ACTGCTGCGG
GACAAGTGGC GCAAGTTCGG GGCCGTCTCC TTCTACATCA ACGTGGTCTC CTACCTGTGT
GCCATGGTCA TCTTCACTCT CACCGCCTAC TACCAGCCGC TGGAGGGCAC ACCGCCGTAC
CCTTACCGCA CCACGGTGGA CTACCTGCGG CTGGCTGGCG AGGTCATTAC GCTCTTCACT
GGGGTCCTGT TCTTCTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG
AATTCTCTCT TCATTGATGG CTCCTTCCAG CTGCTCTACT TCATCTACTC TGTCCTGGTG
ATCGTCTCAG CAGCCCTCTA CCTGGCAGGG ATCGAGGCCT ACCTGGCCGT GATGGTCTTT
GCCCTGGTCC TGGGCTGGAT GAATGCCCTT TACTTCACCC GTGGGCTGAA GCTGACGGGG
ACCTATAGCA TCATGATCCA GAAGATTCTC TTCAAGGACC TTTTCCGATT CCTGCTCGTC
TACTTGCTCT TCATGATCGG CTACGCTTCA GCCCTGGTCT CCCTCCTGAA CCCGTGTGCC
AACATGAAGG TGTGCAATGA GGACCAGACC AACTGCACAG TGCCCACTTA CCCCTCGTGC
CGTGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTGT TTAAGCTGAC CATCGGCATG
GGCGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTG
ACCTACATCA TCCTCACCTT TGTGCTGCTC CTCAACATGC TCATTGCCCT CATGGGCGAG
ACAGTGGGCC AGGTCTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC
ATCCTGGACA TTGAGCGCTC CTTCCCCGTA TTCCTGAGGA AGGCCTTCCG CTCTGGGGAG
ATGGTCACCG TGGGCAAGAG CTCGGACGGC ACTCCTGACC GCAGGTGGTG CTTCAGGGTG
GATGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATCAACGA GGACCCGGGC
AAGAATGAGA CCTACCAGTA TTATGGCTTC TCGCATACCG TGGGCCGCCT CCGCAGGGAT
CGCTGGTCCT CGGTGGTACC CCGCGTGGTG GAACTGAACA AGAACTCGAA CCCGGACGAG
GTGGTGGTGC CTCTGGACAG CATGGGGAAC CCCCGCTGCG ATGGCCACCA GCAGGGTTAC
CCCCGCAAGT GGAGGACTGA TGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_671737.1
CDS32..2467
Misc Feature(1)671..985(+)
Misc Feature(2)743..1180(+)
Misc Feature(3)788..790(+)
Misc Feature(4)1511..2005(+)
Exon (1)1..417
Gene:TRPV4
Gene Synonym:
Exon (2)418..590
Gene:TRPV4
Gene Synonym:
Exon (3)591..743
Gene:TRPV4
Gene Synonym:
Exon (4)744..884
Gene:TRPV4
Gene Synonym:
Exon (5)885..1183
Gene:TRPV4
Gene Synonym:
Exon (6)1184..1342
Gene:TRPV4
Gene Synonym:
Exon (7)1343..1435
Gene:TRPV4
Gene Synonym:
Exon (8)1436..1509
Gene:TRPV4
Gene Synonym:
Exon (9)1510..1675
Gene:TRPV4
Gene Synonym:
Exon (10)1676..1742
Gene:TRPV4
Gene Synonym:
Exon (11)1743..2059
Gene:TRPV4
Gene Synonym:
Exon (12)2060..2187
Gene:TRPV4
Gene Synonym:
Exon (13)2188..2309
Gene:TRPV4
Gene Synonym:
Exon (14)2310..3001
Gene:TRPV4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_147204
Organism Homo sapiens (human)
Definition Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_147204

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG TGGGGAGGCT TTTCCTCTCT CCTCCCTGGC CAATCTGTTT
GAGGGGGAGG ATGGCTCCCT TTCGCCCTCA CCGGCTGATG CCAGTCGCCC TGCTGGCCCA
GGCGATGGGC GACCAAATCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC
AACCCCATCG ATCTGCTGGA GTCCACCCTA TATGAGTCCT CGGTGGTGCC TGGGCCCAAG
AAAGCACCCA TGGACTCACT GTTTGACTAC GGCACCTATC GTCACCACTC CAGTGACAAC
AAGAGGTGGA GGAAGAAGAT CATAGAGAAG CAGCCGCAGA GCCCCAAAGC CCCTGCCCCT
CAGCCGCCCC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG
GGCTCCACTG CTGACCTGGA CGGGCTGCTC CCATTCTTGC TGACCCACAA GAAACGCCTA
ACTGATGAGG AGTTTCGAGA GCCATCTACG GGGAAGACCT GCCTGCCCAA GGCCTTGCTG
AACCTGAGCA ATGGCCGCAA CGACACCATC CCTGTGCTGC TGGACATCGC GGAGCGCACC
GGCAACATGA GGGAGTTCAT TAACTCGCCC TTCCGTGACA TCTACTATCG AGGTCAGACA
GCCCTGCACA TCGCCATTGA GCGTCGCTGC AAACACTACG TGGAACTTCT CGTGGCCCAG
GGAGCTGATG TCCACGCCCA GGCCCGTGGG CGCTTCTTCC AGCCCAAGGA TGAGGGGGGC
TACTTCTACT TTGGGGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATT
GTCAACTACC TGACGGAGAA CCCCCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGA
GGCAACACAG TGCTGCATGC GCTGGTGGCC ATTGCTGACA ACACCCGTGA GAACACCAAG
TTTGTTACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC
AACCTGGAGG CCGTGCTCAA CAACGACGGC CTCTCGCCCC TCATGATGGC TGCCAAGACG
GGCAAGATTG GGAACCGCCA CGAGATGCTG GCTGTGGAGC CCATCAATGA ACTGCTGCGG
GACAAGTGGC GCAAGTTCGG GGCCGTCTCC TTCTACATCA ACGTGGTCTC CTACCTGTGT
GCCATGGTCA TCTTCACTCT CACCGCCTAC TACCAGCCGC TGGAGGGCAC ACCGCCGTAC
CCTTACCGCA CCACGGTGGA CTACCTGCGG CTGGCTGGCG AGGTCATTAC GCTCTTCACT
GGGGTCCTGT TCTTCTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG
AATTCTCTCT TCATTGATGG CTCCTTCCAG CTGCTCTACT TCATCTACTC TGTCCTGGTG
ATCGTCTCAG CAGCCCTCTA CCTGGCAGGG ATCGAGGCCT ACCTGGCCGT GATGGTCTTT
GCCCTGGTCC TGGGCTGGAT GAATGCCCTT TACTTCACCC GTGGGCTGAA GCTGACGGGG
ACCTATAGCA TCATGATCCA GAAGATTCTC TTCAAGGACC TTTTCCGATT CCTGCTCGTC
TACTTGCTCT TCATGATCGG CTACGCTTCA GCCCTGGTCT CCCTCCTGAA CCCGTGTGCC
AACATGAAGG TGTGCAATGA GGACCAGACC AACTGCACAG TGCCCACTTA CCCCTCGTGC
CGTGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTGT TTAAGCTGAC CATCGGCATG
GGCGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTG
ACCTACATCA TCCTCACCTT TGTGCTGCTC CTCAACATGC TCATTGCCCT CATGGGCGAG
ACAGTGGGCC AGGTCTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC
ATCCTGGACA TTGAGCGCTC CTTCCCCGTA TTCCTGAGGA AGGCCTTCCG CTCTGGGGAG
ATGGTCACCG TGGGCAAGAG CTCGGACGGC ACTCCTGACC GCAGGTGGTG CTTCAGGGTG
GATGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATCAACGA GGACCCGGGC
AAGAATGAGA CCTACCAGTA TTATGGCTTC TCGCATACCG TGGGCCGCCT CCGCAGGGAT
CGCTGGTCCT CGGTGGTACC CCGCGTGGTG GAACTGAACA AGAACTCGAA CCCGGACGAG
GTGGTGGTGC CTCTGGACAG CATGGGGAAC CCCCGCTGCG ATGGCCACCA GCAGGGTTAC
CCCCGCAAGT GGAGGACTGA TGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21707
Clone ID Related Accession (Same CDS sequence) NM_021625 , XM_005253918 , XM_011538630
Accession Version XM_005253918.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2616bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product transient receptor potential cation channel subfamily V member 4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG TGGGGAGGCT TTTCCTCTCT CCTCCCTGGC CAATCTGTTT
GAGGGGGAGG ATGGCTCCCT TTCGCCCTCA CCGGCTGATG CCAGTCGCCC TGCTGGCCCA
GGCGATGGGC GACCAAATCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC
AACCCCATCG ATCTGCTGGA GTCCACCCTA TATGAGTCCT CGGTGGTGCC TGGGCCCAAG
AAAGCACCCA TGGACTCACT GTTTGACTAC GGCACCTATC GTCACCACTC CAGTGACAAC
AAGAGGTGGA GGAAGAAGAT CATAGAGAAG CAGCCGCAGA GCCCCAAAGC CCCTGCCCCT
CAGCCGCCCC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG
GGCTCCACTG CTGACCTGGA CGGGCTGCTC CCATTCTTGC TGACCCACAA GAAACGCCTA
ACTGATGAGG AGTTTCGAGA GCCATCTACG GGGAAGACCT GCCTGCCCAA GGCCTTGCTG
AACCTGAGCA ATGGCCGCAA CGACACCATC CCTGTGCTGC TGGACATCGC GGAGCGCACC
GGCAACATGA GGGAGTTCAT TAACTCGCCC TTCCGTGACA TCTACTATCG AGGTCAGACA
GCCCTGCACA TCGCCATTGA GCGTCGCTGC AAACACTACG TGGAACTTCT CGTGGCCCAG
GGAGCTGATG TCCACGCCCA GGCCCGTGGG CGCTTCTTCC AGCCCAAGGA TGAGGGGGGC
TACTTCTACT TTGGGGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATT
GTCAACTACC TGACGGAGAA CCCCCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGA
GGCAACACAG TGCTGCATGC GCTGGTGGCC ATTGCTGACA ACACCCGTGA GAACACCAAG
TTTGTTACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC
AACCTGGAGG CCGTGCTCAA CAACGACGGC CTCTCGCCCC TCATGATGGC TGCCAAGACG
GGCAAGATTG GGATCTTTCA GCACATCATC CGGCGGGAGG TGACGGATGA GGACACACGG
CACCTGTCCC GCAAGTTCAA GGACTGGGCC TATGGGCCAG TGTATTCCTC GCTTTATGAC
CTCTCCTCCC TGGACACGTG TGGGGAAGAG GCCTCCGTGC TGGAGATCCT GGTGTACAAC
AGCAAGATTG AGAACCGCCA CGAGATGCTG GCTGTGGAGC CCATCAATGA ACTGCTGCGG
GACAAGTGGC GCAAGTTCGG GGCCGTCTCC TTCTACATCA ACGTGGTCTC CTACCTGTGT
GCCATGGTCA TCTTCACTCT CACCGCCTAC TACCAGCCGC TGGAGGGCAC ACCGCCGTAC
CCTTACCGCA CCACGGTGGA CTACCTGCGG CTGGCTGGCG AGGTCATTAC GCTCTTCACT
GGGGTCCTGT TCTTCTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG
AATTCTCTCT TCATTGATGG CTCCTTCCAG CTGCTCTACT TCATCTACTC TGTCCTGGTG
ATCGTCTCAG CAGCCCTCTA CCTGGCAGGG ATCGAGGCCT ACCTGGCCGT GATGGTCTTT
GCCCTGGTCC TGGGCTGGAT GAATGCCCTT TACTTCACCC GTGGGCTGAA GCTGACGGGG
ACCTATAGCA TCATGATCCA GAAGATTCTC TTCAAGGACC TTTTCCGATT CCTGCTCGTC
TACTTGCTCT TCATGATCGG CTACGCTTCA GCCCTGGTCT CCCTCCTGAA CCCGTGTGCC
AACATGAAGG TGTGCAATGA GGACCAGACC AACTGCACAG TGCCCACTTA CCCCTCGTGC
CGTGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTGT TTAAGCTGAC CATCGGCATG
GGCGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTG
ACCTACATCA TCCTCACCTT TGTGCTGCTC CTCAACATGC TCATTGCCCT CATGGGCGAG
ACAGTGGGCC AGGTCTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC
ATCCTGGACA TTGAGCGCTC CTTCCCCGTA TTCCTGAGGA AGGCCTTCCG CTCTGGGGAG
ATGGTCACCG TGGGCAAGAG CTCGGACGGC ACTCCTGACC GCAGGTGGTG CTTCAGGGTG
GATGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATCAACGA GGACCCGGGC
AAGAATGAGA CCTACCAGTA TTATGGCTTC TCGCATACCG TGGGCCGCCT CCGCAGGGAT
CGCTGGTCCT CGGTGGTACC CCGCGTGGTG GAACTGAACA AGAACTCGAA CCCGGACGAG
GTGGTGGTGC CTCTGGACAG CATGGGGAAC CCCCGCTGCG ATGGCCACCA GCAGGGTTAC
CCCCGCAAGT GGAGGACTGA TGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005253975.1
CDS450..3065
Misc Feature(1)1089..1403(+)
Misc Feature(2)1161..1619(+)
Misc Feature(3)2109..2603(+)
Translation

Target ORF information:

RefSeq Version XM_005253918
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005253918

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG TGGGGAGGCT TTTCCTCTCT CCTCCCTGGC CAATCTGTTT
GAGGGGGAGG ATGGCTCCCT TTCGCCCTCA CCGGCTGATG CCAGTCGCCC TGCTGGCCCA
GGCGATGGGC GACCAAATCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC
AACCCCATCG ATCTGCTGGA GTCCACCCTA TATGAGTCCT CGGTGGTGCC TGGGCCCAAG
AAAGCACCCA TGGACTCACT GTTTGACTAC GGCACCTATC GTCACCACTC CAGTGACAAC
AAGAGGTGGA GGAAGAAGAT CATAGAGAAG CAGCCGCAGA GCCCCAAAGC CCCTGCCCCT
CAGCCGCCCC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG
GGCTCCACTG CTGACCTGGA CGGGCTGCTC CCATTCTTGC TGACCCACAA GAAACGCCTA
ACTGATGAGG AGTTTCGAGA GCCATCTACG GGGAAGACCT GCCTGCCCAA GGCCTTGCTG
AACCTGAGCA ATGGCCGCAA CGACACCATC CCTGTGCTGC TGGACATCGC GGAGCGCACC
GGCAACATGA GGGAGTTCAT TAACTCGCCC TTCCGTGACA TCTACTATCG AGGTCAGACA
GCCCTGCACA TCGCCATTGA GCGTCGCTGC AAACACTACG TGGAACTTCT CGTGGCCCAG
GGAGCTGATG TCCACGCCCA GGCCCGTGGG CGCTTCTTCC AGCCCAAGGA TGAGGGGGGC
TACTTCTACT TTGGGGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATT
GTCAACTACC TGACGGAGAA CCCCCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGA
GGCAACACAG TGCTGCATGC GCTGGTGGCC ATTGCTGACA ACACCCGTGA GAACACCAAG
TTTGTTACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC
AACCTGGAGG CCGTGCTCAA CAACGACGGC CTCTCGCCCC TCATGATGGC TGCCAAGACG
GGCAAGATTG GGATCTTTCA GCACATCATC CGGCGGGAGG TGACGGATGA GGACACACGG
CACCTGTCCC GCAAGTTCAA GGACTGGGCC TATGGGCCAG TGTATTCCTC GCTTTATGAC
CTCTCCTCCC TGGACACGTG TGGGGAAGAG GCCTCCGTGC TGGAGATCCT GGTGTACAAC
AGCAAGATTG AGAACCGCCA CGAGATGCTG GCTGTGGAGC CCATCAATGA ACTGCTGCGG
GACAAGTGGC GCAAGTTCGG GGCCGTCTCC TTCTACATCA ACGTGGTCTC CTACCTGTGT
GCCATGGTCA TCTTCACTCT CACCGCCTAC TACCAGCCGC TGGAGGGCAC ACCGCCGTAC
CCTTACCGCA CCACGGTGGA CTACCTGCGG CTGGCTGGCG AGGTCATTAC GCTCTTCACT
GGGGTCCTGT TCTTCTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG
AATTCTCTCT TCATTGATGG CTCCTTCCAG CTGCTCTACT TCATCTACTC TGTCCTGGTG
ATCGTCTCAG CAGCCCTCTA CCTGGCAGGG ATCGAGGCCT ACCTGGCCGT GATGGTCTTT
GCCCTGGTCC TGGGCTGGAT GAATGCCCTT TACTTCACCC GTGGGCTGAA GCTGACGGGG
ACCTATAGCA TCATGATCCA GAAGATTCTC TTCAAGGACC TTTTCCGATT CCTGCTCGTC
TACTTGCTCT TCATGATCGG CTACGCTTCA GCCCTGGTCT CCCTCCTGAA CCCGTGTGCC
AACATGAAGG TGTGCAATGA GGACCAGACC AACTGCACAG TGCCCACTTA CCCCTCGTGC
CGTGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTGT TTAAGCTGAC CATCGGCATG
GGCGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTG
ACCTACATCA TCCTCACCTT TGTGCTGCTC CTCAACATGC TCATTGCCCT CATGGGCGAG
ACAGTGGGCC AGGTCTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC
ATCCTGGACA TTGAGCGCTC CTTCCCCGTA TTCCTGAGGA AGGCCTTCCG CTCTGGGGAG
ATGGTCACCG TGGGCAAGAG CTCGGACGGC ACTCCTGACC GCAGGTGGTG CTTCAGGGTG
GATGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATCAACGA GGACCCGGGC
AAGAATGAGA CCTACCAGTA TTATGGCTTC TCGCATACCG TGGGCCGCCT CCGCAGGGAT
CGCTGGTCCT CGGTGGTACC CCGCGTGGTG GAACTGAACA AGAACTCGAA CCCGGACGAG
GTGGTGGTGC CTCTGGACAG CATGGGGAAC CCCCGCTGCG ATGGCCACCA GCAGGGTTAC
CCCCGCAAGT GGAGGACTGA TGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21707
Clone ID Related Accession (Same CDS sequence) NM_021625 , XM_005253918 , XM_011538630
Accession Version XM_011538630.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2616bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product transient receptor potential cation channel subfamily V member 4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG TGGGGAGGCT TTTCCTCTCT CCTCCCTGGC CAATCTGTTT
GAGGGGGAGG ATGGCTCCCT TTCGCCCTCA CCGGCTGATG CCAGTCGCCC TGCTGGCCCA
GGCGATGGGC GACCAAATCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC
AACCCCATCG ATCTGCTGGA GTCCACCCTA TATGAGTCCT CGGTGGTGCC TGGGCCCAAG
AAAGCACCCA TGGACTCACT GTTTGACTAC GGCACCTATC GTCACCACTC CAGTGACAAC
AAGAGGTGGA GGAAGAAGAT CATAGAGAAG CAGCCGCAGA GCCCCAAAGC CCCTGCCCCT
CAGCCGCCCC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG
GGCTCCACTG CTGACCTGGA CGGGCTGCTC CCATTCTTGC TGACCCACAA GAAACGCCTA
ACTGATGAGG AGTTTCGAGA GCCATCTACG GGGAAGACCT GCCTGCCCAA GGCCTTGCTG
AACCTGAGCA ATGGCCGCAA CGACACCATC CCTGTGCTGC TGGACATCGC GGAGCGCACC
GGCAACATGA GGGAGTTCAT TAACTCGCCC TTCCGTGACA TCTACTATCG AGGTCAGACA
GCCCTGCACA TCGCCATTGA GCGTCGCTGC AAACACTACG TGGAACTTCT CGTGGCCCAG
GGAGCTGATG TCCACGCCCA GGCCCGTGGG CGCTTCTTCC AGCCCAAGGA TGAGGGGGGC
TACTTCTACT TTGGGGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATT
GTCAACTACC TGACGGAGAA CCCCCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGA
GGCAACACAG TGCTGCATGC GCTGGTGGCC ATTGCTGACA ACACCCGTGA GAACACCAAG
TTTGTTACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC
AACCTGGAGG CCGTGCTCAA CAACGACGGC CTCTCGCCCC TCATGATGGC TGCCAAGACG
GGCAAGATTG GGATCTTTCA GCACATCATC CGGCGGGAGG TGACGGATGA GGACACACGG
CACCTGTCCC GCAAGTTCAA GGACTGGGCC TATGGGCCAG TGTATTCCTC GCTTTATGAC
CTCTCCTCCC TGGACACGTG TGGGGAAGAG GCCTCCGTGC TGGAGATCCT GGTGTACAAC
AGCAAGATTG AGAACCGCCA CGAGATGCTG GCTGTGGAGC CCATCAATGA ACTGCTGCGG
GACAAGTGGC GCAAGTTCGG GGCCGTCTCC TTCTACATCA ACGTGGTCTC CTACCTGTGT
GCCATGGTCA TCTTCACTCT CACCGCCTAC TACCAGCCGC TGGAGGGCAC ACCGCCGTAC
CCTTACCGCA CCACGGTGGA CTACCTGCGG CTGGCTGGCG AGGTCATTAC GCTCTTCACT
GGGGTCCTGT TCTTCTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG
AATTCTCTCT TCATTGATGG CTCCTTCCAG CTGCTCTACT TCATCTACTC TGTCCTGGTG
ATCGTCTCAG CAGCCCTCTA CCTGGCAGGG ATCGAGGCCT ACCTGGCCGT GATGGTCTTT
GCCCTGGTCC TGGGCTGGAT GAATGCCCTT TACTTCACCC GTGGGCTGAA GCTGACGGGG
ACCTATAGCA TCATGATCCA GAAGATTCTC TTCAAGGACC TTTTCCGATT CCTGCTCGTC
TACTTGCTCT TCATGATCGG CTACGCTTCA GCCCTGGTCT CCCTCCTGAA CCCGTGTGCC
AACATGAAGG TGTGCAATGA GGACCAGACC AACTGCACAG TGCCCACTTA CCCCTCGTGC
CGTGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTGT TTAAGCTGAC CATCGGCATG
GGCGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTG
ACCTACATCA TCCTCACCTT TGTGCTGCTC CTCAACATGC TCATTGCCCT CATGGGCGAG
ACAGTGGGCC AGGTCTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC
ATCCTGGACA TTGAGCGCTC CTTCCCCGTA TTCCTGAGGA AGGCCTTCCG CTCTGGGGAG
ATGGTCACCG TGGGCAAGAG CTCGGACGGC ACTCCTGACC GCAGGTGGTG CTTCAGGGTG
GATGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATCAACGA GGACCCGGGC
AAGAATGAGA CCTACCAGTA TTATGGCTTC TCGCATACCG TGGGCCGCCT CCGCAGGGAT
CGCTGGTCCT CGGTGGTACC CCGCGTGGTG GAACTGAACA AGAACTCGAA CCCGGACGAG
GTGGTGGTGC CTCTGGACAG CATGGGGAAC CCCCGCTGCG ATGGCCACCA GCAGGGTTAC
CCCCGCAAGT GGAGGACTGA TGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011536932.1
CDS138..2753
Misc Feature(1)777..1091(+)
Misc Feature(2)849..1307(+)
Misc Feature(3)1797..2291(+)
Translation

Target ORF information:

RefSeq Version XM_011538630
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011538630

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG TGGGGAGGCT TTTCCTCTCT CCTCCCTGGC CAATCTGTTT
GAGGGGGAGG ATGGCTCCCT TTCGCCCTCA CCGGCTGATG CCAGTCGCCC TGCTGGCCCA
GGCGATGGGC GACCAAATCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC
AACCCCATCG ATCTGCTGGA GTCCACCCTA TATGAGTCCT CGGTGGTGCC TGGGCCCAAG
AAAGCACCCA TGGACTCACT GTTTGACTAC GGCACCTATC GTCACCACTC CAGTGACAAC
AAGAGGTGGA GGAAGAAGAT CATAGAGAAG CAGCCGCAGA GCCCCAAAGC CCCTGCCCCT
CAGCCGCCCC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG
GGCTCCACTG CTGACCTGGA CGGGCTGCTC CCATTCTTGC TGACCCACAA GAAACGCCTA
ACTGATGAGG AGTTTCGAGA GCCATCTACG GGGAAGACCT GCCTGCCCAA GGCCTTGCTG
AACCTGAGCA ATGGCCGCAA CGACACCATC CCTGTGCTGC TGGACATCGC GGAGCGCACC
GGCAACATGA GGGAGTTCAT TAACTCGCCC TTCCGTGACA TCTACTATCG AGGTCAGACA
GCCCTGCACA TCGCCATTGA GCGTCGCTGC AAACACTACG TGGAACTTCT CGTGGCCCAG
GGAGCTGATG TCCACGCCCA GGCCCGTGGG CGCTTCTTCC AGCCCAAGGA TGAGGGGGGC
TACTTCTACT TTGGGGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATT
GTCAACTACC TGACGGAGAA CCCCCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGA
GGCAACACAG TGCTGCATGC GCTGGTGGCC ATTGCTGACA ACACCCGTGA GAACACCAAG
TTTGTTACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC
AACCTGGAGG CCGTGCTCAA CAACGACGGC CTCTCGCCCC TCATGATGGC TGCCAAGACG
GGCAAGATTG GGATCTTTCA GCACATCATC CGGCGGGAGG TGACGGATGA GGACACACGG
CACCTGTCCC GCAAGTTCAA GGACTGGGCC TATGGGCCAG TGTATTCCTC GCTTTATGAC
CTCTCCTCCC TGGACACGTG TGGGGAAGAG GCCTCCGTGC TGGAGATCCT GGTGTACAAC
AGCAAGATTG AGAACCGCCA CGAGATGCTG GCTGTGGAGC CCATCAATGA ACTGCTGCGG
GACAAGTGGC GCAAGTTCGG GGCCGTCTCC TTCTACATCA ACGTGGTCTC CTACCTGTGT
GCCATGGTCA TCTTCACTCT CACCGCCTAC TACCAGCCGC TGGAGGGCAC ACCGCCGTAC
CCTTACCGCA CCACGGTGGA CTACCTGCGG CTGGCTGGCG AGGTCATTAC GCTCTTCACT
GGGGTCCTGT TCTTCTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG
AATTCTCTCT TCATTGATGG CTCCTTCCAG CTGCTCTACT TCATCTACTC TGTCCTGGTG
ATCGTCTCAG CAGCCCTCTA CCTGGCAGGG ATCGAGGCCT ACCTGGCCGT GATGGTCTTT
GCCCTGGTCC TGGGCTGGAT GAATGCCCTT TACTTCACCC GTGGGCTGAA GCTGACGGGG
ACCTATAGCA TCATGATCCA GAAGATTCTC TTCAAGGACC TTTTCCGATT CCTGCTCGTC
TACTTGCTCT TCATGATCGG CTACGCTTCA GCCCTGGTCT CCCTCCTGAA CCCGTGTGCC
AACATGAAGG TGTGCAATGA GGACCAGACC AACTGCACAG TGCCCACTTA CCCCTCGTGC
CGTGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTGT TTAAGCTGAC CATCGGCATG
GGCGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTG
ACCTACATCA TCCTCACCTT TGTGCTGCTC CTCAACATGC TCATTGCCCT CATGGGCGAG
ACAGTGGGCC AGGTCTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC
ATCCTGGACA TTGAGCGCTC CTTCCCCGTA TTCCTGAGGA AGGCCTTCCG CTCTGGGGAG
ATGGTCACCG TGGGCAAGAG CTCGGACGGC ACTCCTGACC GCAGGTGGTG CTTCAGGGTG
GATGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATCAACGA GGACCCGGGC
AAGAATGAGA CCTACCAGTA TTATGGCTTC TCGCATACCG TGGGCCGCCT CCGCAGGGAT
CGCTGGTCCT CGGTGGTACC CCGCGTGGTG GAACTGAACA AGAACTCGAA CCCGGACGAG
GTGGTGGTGC CTCTGGACAG CATGGGGAAC CCCCGCTGCG ATGGCCACCA GCAGGGTTAC
CCCCGCAAGT GGAGGACTGA TGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22834
Clone ID Related Accession (Same CDS sequence) NM_001177428 , XM_011538631
Accession Version XM_011538631.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2475bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product transient receptor potential cation channel subfamily V member 4 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG TGGGGAGGCT TTTCCTCTCT CCTCCCTGGC CAATCTGTTT
GAGGGGGAGG ATGGCTCCCT TTCGCCCTCA CCGGCTGATG CCAGTCGCCC TGCTGGCCCA
GGCGATGGGC GACCAAATCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC
AACCCCATCG ATCTGCTGGA GTCCACCCTA TATGAGTCCT CGGTGGTGCC TGGGCCCAAG
AAAGCACCCA TGGACTCACT GTTTGACTAC GGCACCTATC GTCACCACTC CAGTGACAAC
AAGAGGTGGA GGAAGAAGAT CATAGAGAAG CAGCCGCAGA GCCCCAAAGC CCCTGCCCCT
CAGCCGCCCC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG
GGCTCCACTG CTGACCTGGA CGGGCTGCTC CCATTCTTGC TGACCCACAA GAAACGCCTA
ACTGATGAGG AGTTTCGAGA GCCATCTACG GGGAAGACCT GCCTGCCCAA GGCCTTGCTG
AACCTGAGCA ATGGCCGCAA CGACACCATC CCTGTGCTGC TGGACATCGC GGAGCGCACC
GGCAACATGA GGGAGTTCAT TAACTCGCCC TTCCGTGACA TCTACTATCG AGGGGAGCTG
CCCCTGTCGC TGGCTGCCTG CACCAACCAG CCCCACATTG TCAACTACCT GACGGAGAAC
CCCCACAAGA AGGCGGACAT GCGGCGCCAG GACTCGCGAG GCAACACAGT GCTGCATGCG
CTGGTGGCCA TTGCTGACAA CACCCGTGAG AACACCAAGT TTGTTACCAA GATGTACGAC
CTGCTGCTGC TCAAGTGTGC CCGCCTCTTC CCCGACAGCA ACCTGGAGGC CGTGCTCAAC
AACGACGGCC TCTCGCCCCT CATGATGGCT GCCAAGACGG GCAAGATTGG GATCTTTCAG
CACATCATCC GGCGGGAGGT GACGGATGAG GACACACGGC ACCTGTCCCG CAAGTTCAAG
GACTGGGCCT ATGGGCCAGT GTATTCCTCG CTTTATGACC TCTCCTCCCT GGACACGTGT
GGGGAAGAGG CCTCCGTGCT GGAGATCCTG GTGTACAACA GCAAGATTGA GAACCGCCAC
GAGATGCTGG CTGTGGAGCC CATCAATGAA CTGCTGCGGG ACAAGTGGCG CAAGTTCGGG
GCCGTCTCCT TCTACATCAA CGTGGTCTCC TACCTGTGTG CCATGGTCAT CTTCACTCTC
ACCGCCTACT ACCAGCCGCT GGAGGGCACA CCGCCGTACC CTTACCGCAC CACGGTGGAC
TACCTGCGGC TGGCTGGCGA GGTCATTACG CTCTTCACTG GGGTCCTGTT CTTCTTCACC
AACATCAAAG ACTTGTTCAT GAAGAAATGC CCTGGAGTGA ATTCTCTCTT CATTGATGGC
TCCTTCCAGC TGCTCTACTT CATCTACTCT GTCCTGGTGA TCGTCTCAGC AGCCCTCTAC
CTGGCAGGGA TCGAGGCCTA CCTGGCCGTG ATGGTCTTTG CCCTGGTCCT GGGCTGGATG
AATGCCCTTT ACTTCACCCG TGGGCTGAAG CTGACGGGGA CCTATAGCAT CATGATCCAG
AAGATTCTCT TCAAGGACCT TTTCCGATTC CTGCTCGTCT ACTTGCTCTT CATGATCGGC
TACGCTTCAG CCCTGGTCTC CCTCCTGAAC CCGTGTGCCA ACATGAAGGT GTGCAATGAG
GACCAGACCA ACTGCACAGT GCCCACTTAC CCCTCGTGCC GTGACAGCGA GACCTTCAGC
ACCTTCCTCC TGGACCTGTT TAAGCTGACC ATCGGCATGG GCGACCTGGA GATGCTGAGC
AGCACCAAGT ACCCCGTGGT CTTCATCATC CTGCTGGTGA CCTACATCAT CCTCACCTTT
GTGCTGCTCC TCAACATGCT CATTGCCCTC ATGGGCGAGA CAGTGGGCCA GGTCTCCAAG
GAGAGCAAGC ACATCTGGAA GCTGCAGTGG GCCACCACCA TCCTGGACAT TGAGCGCTCC
TTCCCCGTAT TCCTGAGGAA GGCCTTCCGC TCTGGGGAGA TGGTCACCGT GGGCAAGAGC
TCGGACGGCA CTCCTGACCG CAGGTGGTGC TTCAGGGTGG ATGAGGTGAA CTGGTCTCAC
TGGAACCAGA ACTTGGGCAT CATCAACGAG GACCCGGGCA AGAATGAGAC CTACCAGTAT
TATGGCTTCT CGCATACCGT GGGCCGCCTC CGCAGGGATC GCTGGTCCTC GGTGGTACCC
CGCGTGGTGG AACTGAACAA GAACTCGAAC CCGGACGAGG TGGTGGTGCC TCTGGACAGC
ATGGGGAACC CCCGCTGCGA TGGCCACCAG CAGGGTTACC CCCGCAAGTG GAGGACTGAT
GACGCCCCGC TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011536933.1
CDS139..2613
Misc Feature(1)844..1167(+)
Misc Feature(2)862..1176(+)
Misc Feature(3)1657..2151(+)
Translation

Target ORF information:

RefSeq Version XM_011538631
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011538631

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG TGGGGAGGCT TTTCCTCTCT CCTCCCTGGC CAATCTGTTT
GAGGGGGAGG ATGGCTCCCT TTCGCCCTCA CCGGCTGATG CCAGTCGCCC TGCTGGCCCA
GGCGATGGGC GACCAAATCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC
AACCCCATCG ATCTGCTGGA GTCCACCCTA TATGAGTCCT CGGTGGTGCC TGGGCCCAAG
AAAGCACCCA TGGACTCACT GTTTGACTAC GGCACCTATC GTCACCACTC CAGTGACAAC
AAGAGGTGGA GGAAGAAGAT CATAGAGAAG CAGCCGCAGA GCCCCAAAGC CCCTGCCCCT
CAGCCGCCCC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG
GGCTCCACTG CTGACCTGGA CGGGCTGCTC CCATTCTTGC TGACCCACAA GAAACGCCTA
ACTGATGAGG AGTTTCGAGA GCCATCTACG GGGAAGACCT GCCTGCCCAA GGCCTTGCTG
AACCTGAGCA ATGGCCGCAA CGACACCATC CCTGTGCTGC TGGACATCGC GGAGCGCACC
GGCAACATGA GGGAGTTCAT TAACTCGCCC TTCCGTGACA TCTACTATCG AGGGGAGCTG
CCCCTGTCGC TGGCTGCCTG CACCAACCAG CCCCACATTG TCAACTACCT GACGGAGAAC
CCCCACAAGA AGGCGGACAT GCGGCGCCAG GACTCGCGAG GCAACACAGT GCTGCATGCG
CTGGTGGCCA TTGCTGACAA CACCCGTGAG AACACCAAGT TTGTTACCAA GATGTACGAC
CTGCTGCTGC TCAAGTGTGC CCGCCTCTTC CCCGACAGCA ACCTGGAGGC CGTGCTCAAC
AACGACGGCC TCTCGCCCCT CATGATGGCT GCCAAGACGG GCAAGATTGG GATCTTTCAG
CACATCATCC GGCGGGAGGT GACGGATGAG GACACACGGC ACCTGTCCCG CAAGTTCAAG
GACTGGGCCT ATGGGCCAGT GTATTCCTCG CTTTATGACC TCTCCTCCCT GGACACGTGT
GGGGAAGAGG CCTCCGTGCT GGAGATCCTG GTGTACAACA GCAAGATTGA GAACCGCCAC
GAGATGCTGG CTGTGGAGCC CATCAATGAA CTGCTGCGGG ACAAGTGGCG CAAGTTCGGG
GCCGTCTCCT TCTACATCAA CGTGGTCTCC TACCTGTGTG CCATGGTCAT CTTCACTCTC
ACCGCCTACT ACCAGCCGCT GGAGGGCACA CCGCCGTACC CTTACCGCAC CACGGTGGAC
TACCTGCGGC TGGCTGGCGA GGTCATTACG CTCTTCACTG GGGTCCTGTT CTTCTTCACC
AACATCAAAG ACTTGTTCAT GAAGAAATGC CCTGGAGTGA ATTCTCTCTT CATTGATGGC
TCCTTCCAGC TGCTCTACTT CATCTACTCT GTCCTGGTGA TCGTCTCAGC AGCCCTCTAC
CTGGCAGGGA TCGAGGCCTA CCTGGCCGTG ATGGTCTTTG CCCTGGTCCT GGGCTGGATG
AATGCCCTTT ACTTCACCCG TGGGCTGAAG CTGACGGGGA CCTATAGCAT CATGATCCAG
AAGATTCTCT TCAAGGACCT TTTCCGATTC CTGCTCGTCT ACTTGCTCTT CATGATCGGC
TACGCTTCAG CCCTGGTCTC CCTCCTGAAC CCGTGTGCCA ACATGAAGGT GTGCAATGAG
GACCAGACCA ACTGCACAGT GCCCACTTAC CCCTCGTGCC GTGACAGCGA GACCTTCAGC
ACCTTCCTCC TGGACCTGTT TAAGCTGACC ATCGGCATGG GCGACCTGGA GATGCTGAGC
AGCACCAAGT ACCCCGTGGT CTTCATCATC CTGCTGGTGA CCTACATCAT CCTCACCTTT
GTGCTGCTCC TCAACATGCT CATTGCCCTC ATGGGCGAGA CAGTGGGCCA GGTCTCCAAG
GAGAGCAAGC ACATCTGGAA GCTGCAGTGG GCCACCACCA TCCTGGACAT TGAGCGCTCC
TTCCCCGTAT TCCTGAGGAA GGCCTTCCGC TCTGGGGAGA TGGTCACCGT GGGCAAGAGC
TCGGACGGCA CTCCTGACCG CAGGTGGTGC TTCAGGGTGG ATGAGGTGAA CTGGTCTCAC
TGGAACCAGA ACTTGGGCAT CATCAACGAG GACCCGGGCA AGAATGAGAC CTACCAGTAT
TATGGCTTCT CGCATACCGT GGGCCGCCTC CGCAGGGATC GCTGGTCCTC GGTGGTACCC
CGCGTGGTGG AACTGAACAA GAACTCGAAC CCGGACGAGG TGGTGGTGCC TCTGGACAGC
ATGGGGAACC CCCGCTGCGA TGGCCACCAG CAGGGTTACC CCCGCAAGTG GAGGACTGAT
GACGCCCCGC TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23056
Clone ID Related Accession (Same CDS sequence) NM_147204 , XM_011538632
Accession Version XM_011538632.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2436bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product transient receptor potential cation channel subfamily V member 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG TGGGGAGGCT TTTCCTCTCT CCTCCCTGGC CAATCTGTTT
GAGGGGGAGG ATGGCTCCCT TTCGCCCTCA CCGGCTGATG CCAGTCGCCC TGCTGGCCCA
GGCGATGGGC GACCAAATCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC
AACCCCATCG ATCTGCTGGA GTCCACCCTA TATGAGTCCT CGGTGGTGCC TGGGCCCAAG
AAAGCACCCA TGGACTCACT GTTTGACTAC GGCACCTATC GTCACCACTC CAGTGACAAC
AAGAGGTGGA GGAAGAAGAT CATAGAGAAG CAGCCGCAGA GCCCCAAAGC CCCTGCCCCT
CAGCCGCCCC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG
GGCTCCACTG CTGACCTGGA CGGGCTGCTC CCATTCTTGC TGACCCACAA GAAACGCCTA
ACTGATGAGG AGTTTCGAGA GCCATCTACG GGGAAGACCT GCCTGCCCAA GGCCTTGCTG
AACCTGAGCA ATGGCCGCAA CGACACCATC CCTGTGCTGC TGGACATCGC GGAGCGCACC
GGCAACATGA GGGAGTTCAT TAACTCGCCC TTCCGTGACA TCTACTATCG AGGTCAGACA
GCCCTGCACA TCGCCATTGA GCGTCGCTGC AAACACTACG TGGAACTTCT CGTGGCCCAG
GGAGCTGATG TCCACGCCCA GGCCCGTGGG CGCTTCTTCC AGCCCAAGGA TGAGGGGGGC
TACTTCTACT TTGGGGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATT
GTCAACTACC TGACGGAGAA CCCCCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGA
GGCAACACAG TGCTGCATGC GCTGGTGGCC ATTGCTGACA ACACCCGTGA GAACACCAAG
TTTGTTACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC
AACCTGGAGG CCGTGCTCAA CAACGACGGC CTCTCGCCCC TCATGATGGC TGCCAAGACG
GGCAAGATTG GGAACCGCCA CGAGATGCTG GCTGTGGAGC CCATCAATGA ACTGCTGCGG
GACAAGTGGC GCAAGTTCGG GGCCGTCTCC TTCTACATCA ACGTGGTCTC CTACCTGTGT
GCCATGGTCA TCTTCACTCT CACCGCCTAC TACCAGCCGC TGGAGGGCAC ACCGCCGTAC
CCTTACCGCA CCACGGTGGA CTACCTGCGG CTGGCTGGCG AGGTCATTAC GCTCTTCACT
GGGGTCCTGT TCTTCTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG
AATTCTCTCT TCATTGATGG CTCCTTCCAG CTGCTCTACT TCATCTACTC TGTCCTGGTG
ATCGTCTCAG CAGCCCTCTA CCTGGCAGGG ATCGAGGCCT ACCTGGCCGT GATGGTCTTT
GCCCTGGTCC TGGGCTGGAT GAATGCCCTT TACTTCACCC GTGGGCTGAA GCTGACGGGG
ACCTATAGCA TCATGATCCA GAAGATTCTC TTCAAGGACC TTTTCCGATT CCTGCTCGTC
TACTTGCTCT TCATGATCGG CTACGCTTCA GCCCTGGTCT CCCTCCTGAA CCCGTGTGCC
AACATGAAGG TGTGCAATGA GGACCAGACC AACTGCACAG TGCCCACTTA CCCCTCGTGC
CGTGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTGT TTAAGCTGAC CATCGGCATG
GGCGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTG
ACCTACATCA TCCTCACCTT TGTGCTGCTC CTCAACATGC TCATTGCCCT CATGGGCGAG
ACAGTGGGCC AGGTCTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC
ATCCTGGACA TTGAGCGCTC CTTCCCCGTA TTCCTGAGGA AGGCCTTCCG CTCTGGGGAG
ATGGTCACCG TGGGCAAGAG CTCGGACGGC ACTCCTGACC GCAGGTGGTG CTTCAGGGTG
GATGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATCAACGA GGACCCGGGC
AAGAATGAGA CCTACCAGTA TTATGGCTTC TCGCATACCG TGGGCCGCCT CCGCAGGGAT
CGCTGGTCCT CGGTGGTACC CCGCGTGGTG GAACTGAACA AGAACTCGAA CCCGGACGAG
GTGGTGGTGC CTCTGGACAG CATGGGGAAC CCCCGCTGCG ATGGCCACCA GCAGGGTTAC
CCCCGCAAGT GGAGGACTGA TGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011536934.1
CDS139..2574
Misc Feature(1)778..1092(+)
Misc Feature(2)850..1287(+)
Misc Feature(3)1618..2112(+)
Translation

Target ORF information:

RefSeq Version XM_011538632
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011538632

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG TGGGGAGGCT TTTCCTCTCT CCTCCCTGGC CAATCTGTTT
GAGGGGGAGG ATGGCTCCCT TTCGCCCTCA CCGGCTGATG CCAGTCGCCC TGCTGGCCCA
GGCGATGGGC GACCAAATCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC
AACCCCATCG ATCTGCTGGA GTCCACCCTA TATGAGTCCT CGGTGGTGCC TGGGCCCAAG
AAAGCACCCA TGGACTCACT GTTTGACTAC GGCACCTATC GTCACCACTC CAGTGACAAC
AAGAGGTGGA GGAAGAAGAT CATAGAGAAG CAGCCGCAGA GCCCCAAAGC CCCTGCCCCT
CAGCCGCCCC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG
GGCTCCACTG CTGACCTGGA CGGGCTGCTC CCATTCTTGC TGACCCACAA GAAACGCCTA
ACTGATGAGG AGTTTCGAGA GCCATCTACG GGGAAGACCT GCCTGCCCAA GGCCTTGCTG
AACCTGAGCA ATGGCCGCAA CGACACCATC CCTGTGCTGC TGGACATCGC GGAGCGCACC
GGCAACATGA GGGAGTTCAT TAACTCGCCC TTCCGTGACA TCTACTATCG AGGTCAGACA
GCCCTGCACA TCGCCATTGA GCGTCGCTGC AAACACTACG TGGAACTTCT CGTGGCCCAG
GGAGCTGATG TCCACGCCCA GGCCCGTGGG CGCTTCTTCC AGCCCAAGGA TGAGGGGGGC
TACTTCTACT TTGGGGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATT
GTCAACTACC TGACGGAGAA CCCCCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGA
GGCAACACAG TGCTGCATGC GCTGGTGGCC ATTGCTGACA ACACCCGTGA GAACACCAAG
TTTGTTACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC
AACCTGGAGG CCGTGCTCAA CAACGACGGC CTCTCGCCCC TCATGATGGC TGCCAAGACG
GGCAAGATTG GGAACCGCCA CGAGATGCTG GCTGTGGAGC CCATCAATGA ACTGCTGCGG
GACAAGTGGC GCAAGTTCGG GGCCGTCTCC TTCTACATCA ACGTGGTCTC CTACCTGTGT
GCCATGGTCA TCTTCACTCT CACCGCCTAC TACCAGCCGC TGGAGGGCAC ACCGCCGTAC
CCTTACCGCA CCACGGTGGA CTACCTGCGG CTGGCTGGCG AGGTCATTAC GCTCTTCACT
GGGGTCCTGT TCTTCTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG
AATTCTCTCT TCATTGATGG CTCCTTCCAG CTGCTCTACT TCATCTACTC TGTCCTGGTG
ATCGTCTCAG CAGCCCTCTA CCTGGCAGGG ATCGAGGCCT ACCTGGCCGT GATGGTCTTT
GCCCTGGTCC TGGGCTGGAT GAATGCCCTT TACTTCACCC GTGGGCTGAA GCTGACGGGG
ACCTATAGCA TCATGATCCA GAAGATTCTC TTCAAGGACC TTTTCCGATT CCTGCTCGTC
TACTTGCTCT TCATGATCGG CTACGCTTCA GCCCTGGTCT CCCTCCTGAA CCCGTGTGCC
AACATGAAGG TGTGCAATGA GGACCAGACC AACTGCACAG TGCCCACTTA CCCCTCGTGC
CGTGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTGT TTAAGCTGAC CATCGGCATG
GGCGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTG
ACCTACATCA TCCTCACCTT TGTGCTGCTC CTCAACATGC TCATTGCCCT CATGGGCGAG
ACAGTGGGCC AGGTCTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGTG GGCCACCACC
ATCCTGGACA TTGAGCGCTC CTTCCCCGTA TTCCTGAGGA AGGCCTTCCG CTCTGGGGAG
ATGGTCACCG TGGGCAAGAG CTCGGACGGC ACTCCTGACC GCAGGTGGTG CTTCAGGGTG
GATGAGGTGA ACTGGTCTCA CTGGAACCAG AACTTGGGCA TCATCAACGA GGACCCGGGC
AAGAATGAGA CCTACCAGTA TTATGGCTTC TCGCATACCG TGGGCCGCCT CCGCAGGGAT
CGCTGGTCCT CGGTGGTACC CCGCGTGGTG GAACTGAACA AGAACTCGAA CCCGGACGAG
GTGGTGGTGC CTCTGGACAG CATGGGGAAC CCCCGCTGCG ATGGCCACCA GCAGGGTTAC
CCCCGCAAGT GGAGGACTGA TGACGCCCCG CTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27266
Clone ID Related Accession (Same CDS sequence) NM_001177433 , XM_011538633
Accession Version XM_011538633.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2295bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product transient receptor potential cation channel subfamily V member 4 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG TGGGGAGGCT TTTCCTCTCT CCTCCCTGGC CAATCTGTTT
GAGGGGGAGG ATGGCTCCCT TTCGCCCTCA CCGGCTGATG CCAGTCGCCC TGCTGGCCCA
GGCGATGGGC GACCAAATCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC
AACCCCATCG ATCTGCTGGA GTCCACCCTA TATGAGTCCT CGGTGGTGCC TGGGCCCAAG
AAAGCACCCA TGGACTCACT GTTTGACTAC GGCACCTATC GTCACCACTC CAGTGACAAC
AAGAGGTGGA GGAAGAAGAT CATAGAGAAG CAGCCGCAGA GCCCCAAAGC CCCTGCCCCT
CAGCCGCCCC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG
GGCTCCACTG CTGACCTGGA CGGGCTGCTC CCATTCTTGC TGACCCACAA GAAACGCCTA
ACTGATGAGG AGTTTCGAGA GCCATCTACG GGGAAGACCT GCCTGCCCAA GGCCTTGCTG
AACCTGAGCA ATGGCCGCAA CGACACCATC CCTGTGCTGC TGGACATCGC GGAGCGCACC
GGCAACATGA GGGAGTTCAT TAACTCGCCC TTCCGTGACA TCTACTATCG AGGGGAGCTG
CCCCTGTCGC TGGCTGCCTG CACCAACCAG CCCCACATTG TCAACTACCT GACGGAGAAC
CCCCACAAGA AGGCGGACAT GCGGCGCCAG GACTCGCGAG GCAACACAGT GCTGCATGCG
CTGGTGGCCA TTGCTGACAA CACCCGTGAG AACACCAAGT TTGTTACCAA GATGTACGAC
CTGCTGCTGC TCAAGTGTGC CCGCCTCTTC CCCGACAGCA ACCTGGAGGC CGTGCTCAAC
AACGACGGCC TCTCGCCCCT CATGATGGCT GCCAAGACGG GCAAGATTGG GAACCGCCAC
GAGATGCTGG CTGTGGAGCC CATCAATGAA CTGCTGCGGG ACAAGTGGCG CAAGTTCGGG
GCCGTCTCCT TCTACATCAA CGTGGTCTCC TACCTGTGTG CCATGGTCAT CTTCACTCTC
ACCGCCTACT ACCAGCCGCT GGAGGGCACA CCGCCGTACC CTTACCGCAC CACGGTGGAC
TACCTGCGGC TGGCTGGCGA GGTCATTACG CTCTTCACTG GGGTCCTGTT CTTCTTCACC
AACATCAAAG ACTTGTTCAT GAAGAAATGC CCTGGAGTGA ATTCTCTCTT CATTGATGGC
TCCTTCCAGC TGCTCTACTT CATCTACTCT GTCCTGGTGA TCGTCTCAGC AGCCCTCTAC
CTGGCAGGGA TCGAGGCCTA CCTGGCCGTG ATGGTCTTTG CCCTGGTCCT GGGCTGGATG
AATGCCCTTT ACTTCACCCG TGGGCTGAAG CTGACGGGGA CCTATAGCAT CATGATCCAG
AAGATTCTCT TCAAGGACCT TTTCCGATTC CTGCTCGTCT ACTTGCTCTT CATGATCGGC
TACGCTTCAG CCCTGGTCTC CCTCCTGAAC CCGTGTGCCA ACATGAAGGT GTGCAATGAG
GACCAGACCA ACTGCACAGT GCCCACTTAC CCCTCGTGCC GTGACAGCGA GACCTTCAGC
ACCTTCCTCC TGGACCTGTT TAAGCTGACC ATCGGCATGG GCGACCTGGA GATGCTGAGC
AGCACCAAGT ACCCCGTGGT CTTCATCATC CTGCTGGTGA CCTACATCAT CCTCACCTTT
GTGCTGCTCC TCAACATGCT CATTGCCCTC ATGGGCGAGA CAGTGGGCCA GGTCTCCAAG
GAGAGCAAGC ACATCTGGAA GCTGCAGTGG GCCACCACCA TCCTGGACAT TGAGCGCTCC
TTCCCCGTAT TCCTGAGGAA GGCCTTCCGC TCTGGGGAGA TGGTCACCGT GGGCAAGAGC
TCGGACGGCA CTCCTGACCG CAGGTGGTGC TTCAGGGTGG ATGAGGTGAA CTGGTCTCAC
TGGAACCAGA ACTTGGGCAT CATCAACGAG GACCCGGGCA AGAATGAGAC CTACCAGTAT
TATGGCTTCT CGCATACCGT GGGCCGCCTC CGCAGGGATC GCTGGTCCTC GGTGGTACCC
CGCGTGGTGG AACTGAACAA GAACTCGAAC CCGGACGAGG TGGTGGTGCC TCTGGACAGC
ATGGGGAACC CCCGCTGCGA TGGCCACCAG CAGGGTTACC CCCGCAAGTG GAGGACTGAT
GACGCCCCGC TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011536935.1
CDS139..2433
Misc Feature(1)583..2214(+)
Misc Feature(2)844..1146(+)
Translation

Target ORF information:

RefSeq Version XM_011538633
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011538633

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG TGGGGAGGCT TTTCCTCTCT CCTCCCTGGC CAATCTGTTT
GAGGGGGAGG ATGGCTCCCT TTCGCCCTCA CCGGCTGATG CCAGTCGCCC TGCTGGCCCA
GGCGATGGGC GACCAAATCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC
AACCCCATCG ATCTGCTGGA GTCCACCCTA TATGAGTCCT CGGTGGTGCC TGGGCCCAAG
AAAGCACCCA TGGACTCACT GTTTGACTAC GGCACCTATC GTCACCACTC CAGTGACAAC
AAGAGGTGGA GGAAGAAGAT CATAGAGAAG CAGCCGCAGA GCCCCAAAGC CCCTGCCCCT
CAGCCGCCCC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG
GGCTCCACTG CTGACCTGGA CGGGCTGCTC CCATTCTTGC TGACCCACAA GAAACGCCTA
ACTGATGAGG AGTTTCGAGA GCCATCTACG GGGAAGACCT GCCTGCCCAA GGCCTTGCTG
AACCTGAGCA ATGGCCGCAA CGACACCATC CCTGTGCTGC TGGACATCGC GGAGCGCACC
GGCAACATGA GGGAGTTCAT TAACTCGCCC TTCCGTGACA TCTACTATCG AGGGGAGCTG
CCCCTGTCGC TGGCTGCCTG CACCAACCAG CCCCACATTG TCAACTACCT GACGGAGAAC
CCCCACAAGA AGGCGGACAT GCGGCGCCAG GACTCGCGAG GCAACACAGT GCTGCATGCG
CTGGTGGCCA TTGCTGACAA CACCCGTGAG AACACCAAGT TTGTTACCAA GATGTACGAC
CTGCTGCTGC TCAAGTGTGC CCGCCTCTTC CCCGACAGCA ACCTGGAGGC CGTGCTCAAC
AACGACGGCC TCTCGCCCCT CATGATGGCT GCCAAGACGG GCAAGATTGG GAACCGCCAC
GAGATGCTGG CTGTGGAGCC CATCAATGAA CTGCTGCGGG ACAAGTGGCG CAAGTTCGGG
GCCGTCTCCT TCTACATCAA CGTGGTCTCC TACCTGTGTG CCATGGTCAT CTTCACTCTC
ACCGCCTACT ACCAGCCGCT GGAGGGCACA CCGCCGTACC CTTACCGCAC CACGGTGGAC
TACCTGCGGC TGGCTGGCGA GGTCATTACG CTCTTCACTG GGGTCCTGTT CTTCTTCACC
AACATCAAAG ACTTGTTCAT GAAGAAATGC CCTGGAGTGA ATTCTCTCTT CATTGATGGC
TCCTTCCAGC TGCTCTACTT CATCTACTCT GTCCTGGTGA TCGTCTCAGC AGCCCTCTAC
CTGGCAGGGA TCGAGGCCTA CCTGGCCGTG ATGGTCTTTG CCCTGGTCCT GGGCTGGATG
AATGCCCTTT ACTTCACCCG TGGGCTGAAG CTGACGGGGA CCTATAGCAT CATGATCCAG
AAGATTCTCT TCAAGGACCT TTTCCGATTC CTGCTCGTCT ACTTGCTCTT CATGATCGGC
TACGCTTCAG CCCTGGTCTC CCTCCTGAAC CCGTGTGCCA ACATGAAGGT GTGCAATGAG
GACCAGACCA ACTGCACAGT GCCCACTTAC CCCTCGTGCC GTGACAGCGA GACCTTCAGC
ACCTTCCTCC TGGACCTGTT TAAGCTGACC ATCGGCATGG GCGACCTGGA GATGCTGAGC
AGCACCAAGT ACCCCGTGGT CTTCATCATC CTGCTGGTGA CCTACATCAT CCTCACCTTT
GTGCTGCTCC TCAACATGCT CATTGCCCTC ATGGGCGAGA CAGTGGGCCA GGTCTCCAAG
GAGAGCAAGC ACATCTGGAA GCTGCAGTGG GCCACCACCA TCCTGGACAT TGAGCGCTCC
TTCCCCGTAT TCCTGAGGAA GGCCTTCCGC TCTGGGGAGA TGGTCACCGT GGGCAAGAGC
TCGGACGGCA CTCCTGACCG CAGGTGGTGC TTCAGGGTGG ATGAGGTGAA CTGGTCTCAC
TGGAACCAGA ACTTGGGCAT CATCAACGAG GACCCGGGCA AGAATGAGAC CTACCAGTAT
TATGGCTTCT CGCATACCGT GGGCCGCCTC CGCAGGGATC GCTGGTCCTC GGTGGTACCC
CGCGTGGTGG AACTGAACAA GAACTCGAAC CCGGACGAGG TGGTGGTGCC TCTGGACAGC
ATGGGGAACC CCCGCTGCGA TGGCCACCAG CAGGGTTACC CCCGCAAGTG GAGGACTGAT
GACGCCCCGC TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71031
Clone ID Related Accession (Same CDS sequence) XM_011538634
Accession Version XM_011538634.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2229bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product transient receptor potential cation channel subfamily V member 4 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG TGGGGAGGCT TTTCCTCTCT CCTCCCTGGC CAATCTGTTT
GAGGGGGAGG ATGGCTCCCT TTCGCCCTCA CCGGCTGATG CCAGTCGCCC TGCTGGCCCA
GGCGATGGGC GACCAAATCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC
AACCCCATCG ATCTGCTGGA GTCCACCCTA TATGAGTCCT CGGTGGTGCC TGGGCCCAAG
AAAGCACCCA TGGACTCACT GTTTGACTAC GGCACCTATC GTCACCACTC CAGTGACAAC
AAGAGGTGGA GGAAGAAGAT CATAGAGAAG CAGCCGCAGA GCCCCAAAGC CCCTGCCCCT
CAGCCGCCCC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG
GGCTCCACTG CTGACCTGGA CGGGCTGCTC CCATTCTTGC TGACCCACAA GAAACGCCTA
ACTGATGAGG AGTTTCGAGA GCCATCTACG GGGAAGACCT GCCTGCCCAA GGCCTTGCTG
AACCTGAGCA ATGGCCGCAA CGACACCATC CCTGTGCTGC TGGACATCGC GGAGCGCACC
GGCAACATGA GGGAGTTCAT TAACTCGCCC TTCCGTGACA TCTACTATCG AGGTCAGACA
GCCCTGCACA TCGCCATTGA GCGTCGCTGC AAACACTACG TGGAACTTCT CGTGGCCCAG
GGAGCTGATG TCCACGCCCA GGCCCGTGGG CGCTTCTTCC AGCCCAAGGA TGAGGGGGGC
TACTTCTACT TTGGGGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATT
GTCAACTACC TGACGGAGAA CCCCCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGA
GGCAACACAG TGCTGCATGC GCTGGTGGCC ATTGCTGACA ACACCCGTGA GAACACCAAG
TTTGTTACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC
AACCTGGAGG CCGTGCTCAA CAACGACGGC CTCTCGCCCC TCATGATGGC TGCCAAGACG
GGCAAGATTG GGATCTTTCA GCACATCATC CGGCGGGAGG TGACGGATGA GGACACACGG
CACCTGTCCC GCAAGTTCAA GGACTGGGCC TATGGGCCAG TGTATTCCTC GCTTTATGAC
CTCTCCTCCC TGGACACGTG TGGGGAAGAG GCCTCCGTGC TGGAGATCCT GGTGTACAAC
AGCAAGATTG AGAACCGCCA CGAGATGCTG GCTGTGGAGC CCATCAATGA ACTGCTGCGG
GACAAGTGGC GCAAGTTCGG GGCCGTCTCC TTCTACATCA ACGTGGTCTC CTACCTGTGT
GCCATGGTCA TCTTCACTCT CACCGCCTAC TACCAGCCGC TGGAGGGCAC ACCGCCGTAC
CCTTACCGCA CCACGGTGGA CTACCTGCGG CTGGCTGGCG AGGTCATTAC GCTCTTCACT
GGGGTCCTGT TCTTCTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG
AATTCTCTCT TCATTGATGG CTCCTTCCAG CTGCTCTACT TCATCTACTC TGTCCTGGTG
ATCGTCTCAG CAGCCCTCTA CCTGGCAGGG ATCGAGGCCT ACCTGGCCGT GATGGTCTTT
GCCCTGGTCC TGGGCTGGAT GAATGCCCTT TACTTCACCC GTGGGCTGAA GCTGACGGGG
ACCTATAGCA TCATGATCCA GAAGATTCTC TTCAAGGACC TTTTCCGATT CCTGCTCGTC
TACTTGCTCT TCATGATCGG CTACGCTTCA GCCCTGGTCT CCCTCCTGAA CCCGTGTGCC
AACATGAAGG TGTGCAATGA GGACCAGACC AACTGCACAG TGCCCACTTA CCCCTCGTGC
CGTGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTGT TTAAGCTGAC CATCGGCATG
GGCGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTG
ACCTACATCA TCCTCACCTT TGTGCTGCTC CTCAACATGC TCATTGCCCT CATGGGCGAG
ACAGTGGGCC AGGTCTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGAG CGGCAGGCGC
AGGCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011536936.1
CDS142..2370
Misc Feature(1)781..1095(+)
Misc Feature(2)853..1311(+)
Misc Feature(3)1801..2295(+)
Translation

Target ORF information:

RefSeq Version XM_011538634
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011538634

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCGGATT CCAGCGAAGG CCCCCGCGCG GGGCCCGGGG AGGTGGCTGA GCTCCCCGGG 
GATGAGAGTG GCACCCCAGG TGGGGAGGCT TTTCCTCTCT CCTCCCTGGC CAATCTGTTT
GAGGGGGAGG ATGGCTCCCT TTCGCCCTCA CCGGCTGATG CCAGTCGCCC TGCTGGCCCA
GGCGATGGGC GACCAAATCT GCGCATGAAG TTCCAGGGCG CCTTCCGCAA GGGGGTGCCC
AACCCCATCG ATCTGCTGGA GTCCACCCTA TATGAGTCCT CGGTGGTGCC TGGGCCCAAG
AAAGCACCCA TGGACTCACT GTTTGACTAC GGCACCTATC GTCACCACTC CAGTGACAAC
AAGAGGTGGA GGAAGAAGAT CATAGAGAAG CAGCCGCAGA GCCCCAAAGC CCCTGCCCCT
CAGCCGCCCC CCATCCTCAA AGTCTTCAAC CGGCCTATCC TCTTTGACAT CGTGTCCCGG
GGCTCCACTG CTGACCTGGA CGGGCTGCTC CCATTCTTGC TGACCCACAA GAAACGCCTA
ACTGATGAGG AGTTTCGAGA GCCATCTACG GGGAAGACCT GCCTGCCCAA GGCCTTGCTG
AACCTGAGCA ATGGCCGCAA CGACACCATC CCTGTGCTGC TGGACATCGC GGAGCGCACC
GGCAACATGA GGGAGTTCAT TAACTCGCCC TTCCGTGACA TCTACTATCG AGGTCAGACA
GCCCTGCACA TCGCCATTGA GCGTCGCTGC AAACACTACG TGGAACTTCT CGTGGCCCAG
GGAGCTGATG TCCACGCCCA GGCCCGTGGG CGCTTCTTCC AGCCCAAGGA TGAGGGGGGC
TACTTCTACT TTGGGGAGCT GCCCCTGTCG CTGGCTGCCT GCACCAACCA GCCCCACATT
GTCAACTACC TGACGGAGAA CCCCCACAAG AAGGCGGACA TGCGGCGCCA GGACTCGCGA
GGCAACACAG TGCTGCATGC GCTGGTGGCC ATTGCTGACA ACACCCGTGA GAACACCAAG
TTTGTTACCA AGATGTACGA CCTGCTGCTG CTCAAGTGTG CCCGCCTCTT CCCCGACAGC
AACCTGGAGG CCGTGCTCAA CAACGACGGC CTCTCGCCCC TCATGATGGC TGCCAAGACG
GGCAAGATTG GGATCTTTCA GCACATCATC CGGCGGGAGG TGACGGATGA GGACACACGG
CACCTGTCCC GCAAGTTCAA GGACTGGGCC TATGGGCCAG TGTATTCCTC GCTTTATGAC
CTCTCCTCCC TGGACACGTG TGGGGAAGAG GCCTCCGTGC TGGAGATCCT GGTGTACAAC
AGCAAGATTG AGAACCGCCA CGAGATGCTG GCTGTGGAGC CCATCAATGA ACTGCTGCGG
GACAAGTGGC GCAAGTTCGG GGCCGTCTCC TTCTACATCA ACGTGGTCTC CTACCTGTGT
GCCATGGTCA TCTTCACTCT CACCGCCTAC TACCAGCCGC TGGAGGGCAC ACCGCCGTAC
CCTTACCGCA CCACGGTGGA CTACCTGCGG CTGGCTGGCG AGGTCATTAC GCTCTTCACT
GGGGTCCTGT TCTTCTTCAC CAACATCAAA GACTTGTTCA TGAAGAAATG CCCTGGAGTG
AATTCTCTCT TCATTGATGG CTCCTTCCAG CTGCTCTACT TCATCTACTC TGTCCTGGTG
ATCGTCTCAG CAGCCCTCTA CCTGGCAGGG ATCGAGGCCT ACCTGGCCGT GATGGTCTTT
GCCCTGGTCC TGGGCTGGAT GAATGCCCTT TACTTCACCC GTGGGCTGAA GCTGACGGGG
ACCTATAGCA TCATGATCCA GAAGATTCTC TTCAAGGACC TTTTCCGATT CCTGCTCGTC
TACTTGCTCT TCATGATCGG CTACGCTTCA GCCCTGGTCT CCCTCCTGAA CCCGTGTGCC
AACATGAAGG TGTGCAATGA GGACCAGACC AACTGCACAG TGCCCACTTA CCCCTCGTGC
CGTGACAGCG AGACCTTCAG CACCTTCCTC CTGGACCTGT TTAAGCTGAC CATCGGCATG
GGCGACCTGG AGATGCTGAG CAGCACCAAG TACCCCGTGG TCTTCATCAT CCTGCTGGTG
ACCTACATCA TCCTCACCTT TGTGCTGCTC CTCAACATGC TCATTGCCCT CATGGGCGAG
ACAGTGGGCC AGGTCTCCAA GGAGAGCAAG CACATCTGGA AGCTGCAGAG CGGCAGGCGC
AGGCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71032
Clone ID Related Accession (Same CDS sequence) XM_011538635
Accession Version XM_011538635.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2088bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product transient receptor potential cation channel subfamily V member 4 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGAGAATGG GCGGCAGTAA TCATCCCTTC CTCCTGGGGG TGGTTGTGAA GCTTGAAGGA 
GACACTGTAA GCGCTCGGGT GGTACCCTGG TACTCCATCA CTAACCTGCT GTGTGACCTC
AGGCTGAGCA GTGCAGACGG GCCTGGGGCA GGCATGGCGG ATTCCAGCGA AGGCCCCCGC
GCGGGGCCCG GGGAGGTGGC TGAGCTCCCC GGGGATGAGA GTGGCACCCC AGGTGGGGAG
GCTTTTCCTC TCTCCTCCCT GGCCAATCTG TTTGAGGGGG AGGATGGCTC CCTTTCGCCC
TCACCGGCTG ATGCCAGTCG CCCTGCTGGC CCAGGCGATG GGCGACCAAA TCTGCGCATG
AAGTTCCAGG GCGCCTTCCG CAAGGGGGTG CCCAACCCCA TCGATCTGCT GGAGTCCACC
CTATATGAGT CCTCGGTGGT GCCTGGGCCC AAGAAAGCAC CCATGGACTC ACTGTTTGAC
TACGGCACCT ATCGTCACCA CTCCAGTGAC AACAAGAGGT GGAGGAAGAA GATCATAGAG
AAGCAGCCGC AGAGCCCCAA AGCCCCTGCC CCTCAGCCGC CCCCCATCCT CAAAGTCTTC
AACCGGCCTA TCCTCTTTGA CATCGTGTCC CGGGGCTCCA CTGCTGACCT GGACGGGCTG
CTCCCATTCT TGCTGACCCA CAAGAAACGC CTAACTGATG AGGAGTTTCG AGAGCCATCT
ACGGGGAAGA CCTGCCTGCC CAAGGCCTTG CTGAACCTGA GCAATGGCCG CAACGACACC
ATCCCTGTGC TGCTGGACAT CGCGGAGCGC ACCGGCAACA TGAGGGAGTT CATTAACTCG
CCCTTCCGTG ACATCTACTA TCGAGGTCAG ACAGCCCTGC ACATCGCCAT TGAGCGTCGC
TGCAAACACT ACGTGGAACT TCTCGTGGCC CAGGGAGCTG ATGTCCACGC CCAGGCCCGT
GGGCGCTTCT TCCAGCCCAA GGATGAGGGG GGCTACTTCT ACTTTGGGGA GCTGCCCCTG
TCGCTGGCTG CCTGCACCAA CCAGCCCCAC ATTGTCAACT ACCTGACGGA GAACCCCCAC
AAGAAGGCGG ACATGCGGCG CCAGGACTCG CGAGGCAACA CAGTGCTGCA TGCGCTGGTG
GCCATTGCTG ACAACACCCG TGAGAACACC AAGTTTGTTA CCAAGATGTA CGACCTGCTG
CTGCTCAAGT GTGCCCGCCT CTTCCCCGAC AGCAACCTGG AGGCCGTGCT CAACAACGAC
GGCCTCTCGC CCCTCATGAT GGCTGCCAAG ACGGGCAAGA TTGGGATCTT TCAGCACATC
ATCCGGCGGG AGGTGACGGA TGAGGACACA CGGCACCTGT CCCGCAAGTT CAAGGACTGG
GCCTATGGGC CAGTGTATTC CTCGCTTTAT GACCTCTCCT CCCTGGACAC GTGTGGGGAA
GAGGCCTCCG TGCTGGAGAT CCTGGTGTAC AACAGCAAGA TTGAGAACCG CCACGAGATG
CTGGCTGTGG AGCCCATCAA TGAACTGCTG CGGGACAAGT GGCGCAAGTT CGGGGCCGTC
TCCTTCTACA TCAACGTGGT CTCCTACCTG TGTGCCATGG TCATCTTCAC TCTCACCGCC
TACTACCAGC CGCTGGAGGG CACACCGCCG TACCCTTACC GCACCACGGT GGACTACCTG
CGGCTGGCTG GCGAGGTCAT TACGCTCTTC ACTGGGGTCC TGTTCTTCTT CACCAACATC
AAAGACTTGT TCATGAAGAA ATGCCCTGGA GTGAATTCTC TCTTCATTGA TGGCTCCTTC
CAGCTGCTCT ACTTCATCTA CTCTGTCCTG GTGATCGTCT CAGCAGCCCT CTACCTGGCA
GGGATCGAGG CCTACCTGGC CGTGATGGTC TTTGCCCTGG TCCTGGGCTG GATGAATGCC
CTTTACTTCA CCCGTGGGCT GAAGCTGACG GGGACCTATA GCATCATGAT CCAGAAGATT
CTCTTCAAGG ACCTTTTCCG ATTCCTGCTC GTCTACTTGC TCTTCATGAT CGGCTACGCT
TCAGTGAGTG CCACCCCTAT CCTCCCACAG CCCTGGTCTC CCTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011536937.1
CDS106..2193
Misc Feature(1)898..1212(+)
Misc Feature(2)970..1428(+)
Misc Feature(3)1918..>2154(+)
Translation

Target ORF information:

RefSeq Version XM_011538635
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011538635

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGAGAATGG GCGGCAGTAA TCATCCCTTC CTCCTGGGGG TGGTTGTGAA GCTTGAAGGA 
GACACTGTAA GCGCTCGGGT GGTACCCTGG TACTCCATCA CTAACCTGCT GTGTGACCTC
AGGCTGAGCA GTGCAGACGG GCCTGGGGCA GGCATGGCGG ATTCCAGCGA AGGCCCCCGC
GCGGGGCCCG GGGAGGTGGC TGAGCTCCCC GGGGATGAGA GTGGCACCCC AGGTGGGGAG
GCTTTTCCTC TCTCCTCCCT GGCCAATCTG TTTGAGGGGG AGGATGGCTC CCTTTCGCCC
TCACCGGCTG ATGCCAGTCG CCCTGCTGGC CCAGGCGATG GGCGACCAAA TCTGCGCATG
AAGTTCCAGG GCGCCTTCCG CAAGGGGGTG CCCAACCCCA TCGATCTGCT GGAGTCCACC
CTATATGAGT CCTCGGTGGT GCCTGGGCCC AAGAAAGCAC CCATGGACTC ACTGTTTGAC
TACGGCACCT ATCGTCACCA CTCCAGTGAC AACAAGAGGT GGAGGAAGAA GATCATAGAG
AAGCAGCCGC AGAGCCCCAA AGCCCCTGCC CCTCAGCCGC CCCCCATCCT CAAAGTCTTC
AACCGGCCTA TCCTCTTTGA CATCGTGTCC CGGGGCTCCA CTGCTGACCT GGACGGGCTG
CTCCCATTCT TGCTGACCCA CAAGAAACGC CTAACTGATG AGGAGTTTCG AGAGCCATCT
ACGGGGAAGA CCTGCCTGCC CAAGGCCTTG CTGAACCTGA GCAATGGCCG CAACGACACC
ATCCCTGTGC TGCTGGACAT CGCGGAGCGC ACCGGCAACA TGAGGGAGTT CATTAACTCG
CCCTTCCGTG ACATCTACTA TCGAGGTCAG ACAGCCCTGC ACATCGCCAT TGAGCGTCGC
TGCAAACACT ACGTGGAACT TCTCGTGGCC CAGGGAGCTG ATGTCCACGC CCAGGCCCGT
GGGCGCTTCT TCCAGCCCAA GGATGAGGGG GGCTACTTCT ACTTTGGGGA GCTGCCCCTG
TCGCTGGCTG CCTGCACCAA CCAGCCCCAC ATTGTCAACT ACCTGACGGA GAACCCCCAC
AAGAAGGCGG ACATGCGGCG CCAGGACTCG CGAGGCAACA CAGTGCTGCA TGCGCTGGTG
GCCATTGCTG ACAACACCCG TGAGAACACC AAGTTTGTTA CCAAGATGTA CGACCTGCTG
CTGCTCAAGT GTGCCCGCCT CTTCCCCGAC AGCAACCTGG AGGCCGTGCT CAACAACGAC
GGCCTCTCGC CCCTCATGAT GGCTGCCAAG ACGGGCAAGA TTGGGATCTT TCAGCACATC
ATCCGGCGGG AGGTGACGGA TGAGGACACA CGGCACCTGT CCCGCAAGTT CAAGGACTGG
GCCTATGGGC CAGTGTATTC CTCGCTTTAT GACCTCTCCT CCCTGGACAC GTGTGGGGAA
GAGGCCTCCG TGCTGGAGAT CCTGGTGTAC AACAGCAAGA TTGAGAACCG CCACGAGATG
CTGGCTGTGG AGCCCATCAA TGAACTGCTG CGGGACAAGT GGCGCAAGTT CGGGGCCGTC
TCCTTCTACA TCAACGTGGT CTCCTACCTG TGTGCCATGG TCATCTTCAC TCTCACCGCC
TACTACCAGC CGCTGGAGGG CACACCGCCG TACCCTTACC GCACCACGGT GGACTACCTG
CGGCTGGCTG GCGAGGTCAT TACGCTCTTC ACTGGGGTCC TGTTCTTCTT CACCAACATC
AAAGACTTGT TCATGAAGAA ATGCCCTGGA GTGAATTCTC TCTTCATTGA TGGCTCCTTC
CAGCTGCTCT ACTTCATCTA CTCTGTCCTG GTGATCGTCT CAGCAGCCCT CTACCTGGCA
GGGATCGAGG CCTACCTGGC CGTGATGGTC TTTGCCCTGG TCCTGGGCTG GATGAATGCC
CTTTACTTCA CCCGTGGGCT GAAGCTGACG GGGACCTATA GCATCATGAT CCAGAAGATT
CTCTTCAAGG ACCTTTTCCG ATTCCTGCTC GTCTACTTGC TCTTCATGAT CGGCTACGCT
TCAGTGAGTG CCACCCCTAT CCTCCCACAG CCCTGGTCTC CCTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71033
Clone ID Related Accession (Same CDS sequence) XM_011538636
Accession Version XM_011538636.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1812bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product transient receptor potential cation channel subfamily V member 4 isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGAGAATGG GCGGCAGTAA TCATCCCTTC CTCCTGGGGG TGGTTGTGAA GCTTGAAGGA 
GACACTGTAA GCGCTCGGGT GGTACCCTGG TACTCCATCA CTAACCTGCT GTGTGACCTC
AGGCTGAGCA GTGCAGACGG GCCTGGGGCA GGCATGGCGG ATTCCAGCGA AGGCCCCCGC
GCGGGGCCCG GGGAGGTGGC TGAGCTCCCC GGGGATGAGA GTGGCACCCC AGGTGGGGAG
GCTTTTCCTC TCTCCTCCCT GGCCAATCTG TTTGAGGGGG AGGATGGCTC CCTTTCGCCC
TCACCGGCTG ATGCCAGTCG CCCTGCTGGC CCAGGCGATG GGCGACCAAA TCTGCGCATG
AAGTTCCAGG GCGCCTTCCG CAAGGGGGTG CCCAACCCCA TCGATCTGCT GGAGTCCACC
CTATATGAGT CCTCGGTGGT GCCTGGGCCC AAGAAAGCAC CCATGGACTC ACTGTTTGAC
TACGGCACCT ATCGTCACCA CTCCAGTGAC AACAAGAGGT GGAGGAAGAA GATCATAGAG
AAGCAGCCGC AGAGCCCCAA AGCCCCTGCC CCTCAGCCGC CCCCCATCCT CAAAGTCTTC
AACCGGCCTA TCCTCTTTGA CATCGTGTCC CGGGGCTCCA CTGCTGACCT GGACGGGCTG
CTCCCATTCT TGCTGACCCA CAAGAAACGC CTAACTGATG AGGAGTTTCG AGAGCCATCT
ACGGGGAAGA CCTGCCTGCC CAAGGCCTTG CTGAACCTGA GCAATGGCCG CAACGACACC
ATCCCTGTGC TGCTGGACAT CGCGGAGCGC ACCGGCAACA TGAGGGAGTT CATTAACTCG
CCCTTCCGTG ACATCTACTA TCGAGGTCAG ACAGCCCTGC ACATCGCCAT TGAGCGTCGC
TGCAAACACT ACGTGGAACT TCTCGTGGCC CAGGGAGCTG ATGTCCACGC CCAGGCCCGT
GGGCGCTTCT TCCAGCCCAA GGATGAGGGG GGCTACTTCT ACTTTGGGGA GCTGCCCCTG
TCGCTGGCTG CCTGCACCAA CCAGCCCCAC ATTGTCAACT ACCTGACGGA GAACCCCCAC
AAGAAGGCGG ACATGCGGCG CCAGGACTCG CGAGGCAACA CAGTGCTGCA TGCGCTGGTG
GCCATTGCTG ACAACACCCG TGAGAACACC AAGTTTGTTA CCAAGATGTA CGACCTGCTG
CTGCTCAAGT GTGCCCGCCT CTTCCCCGAC AGCAACCTGG AGGCCGTGCT CAACAACGAC
GGCCTCTCGC CCCTCATGAT GGCTGCCAAG ACGGGCAAGA TTGGGATCTT TCAGCACATC
ATCCGGCGGG AGGTGACGGA TGAGGACACA CGGCACCTGT CCCGCAAGTT CAAGGACTGG
GCCTATGGGC CAGTGTATTC CTCGCTTTAT GACCTCTCCT CCCTGGACAC GTGTGGGGAA
GAGGCCTCCG TGCTGGAGAT CCTGGTGTAC AACAGCAAGA TTGAGAACCG CCACGAGATG
CTGGCTGTGG AGCCCATCAA TGAACTGCTG CGGGACAAGT GGCGCAAGTT CGGGGCCGTC
TCCTTCTACA TCAACGTGGT CTCCTACCTG TGTGCCATGG TCATCTTCAC TCTCACCGCC
TACTACCAGC CGCTGGAGGG CACACCGCCG TACCCTTACC GCACCACGGT GGACTACCTG
CGGCTGGCTG GCGAGGTCAT TACGCTCTTC ACTGGGGTCC TGTTCTTCTT CACCAACATC
AAAGACTTGT TCATGAAGAA ATGCCCTGGA GTGAATTCTC TCTTCATTGA TGGCTCCTTC
CAGCTGCTCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011536938.1
CDS106..1917
Misc Feature(1)898..1212(+)
Misc Feature(2)970..1428(+)
Translation

Target ORF information:

RefSeq Version XM_011538636
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011538636

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGAGAATGG GCGGCAGTAA TCATCCCTTC CTCCTGGGGG TGGTTGTGAA GCTTGAAGGA 
GACACTGTAA GCGCTCGGGT GGTACCCTGG TACTCCATCA CTAACCTGCT GTGTGACCTC
AGGCTGAGCA GTGCAGACGG GCCTGGGGCA GGCATGGCGG ATTCCAGCGA AGGCCCCCGC
GCGGGGCCCG GGGAGGTGGC TGAGCTCCCC GGGGATGAGA GTGGCACCCC AGGTGGGGAG
GCTTTTCCTC TCTCCTCCCT GGCCAATCTG TTTGAGGGGG AGGATGGCTC CCTTTCGCCC
TCACCGGCTG ATGCCAGTCG CCCTGCTGGC CCAGGCGATG GGCGACCAAA TCTGCGCATG
AAGTTCCAGG GCGCCTTCCG CAAGGGGGTG CCCAACCCCA TCGATCTGCT GGAGTCCACC
CTATATGAGT CCTCGGTGGT GCCTGGGCCC AAGAAAGCAC CCATGGACTC ACTGTTTGAC
TACGGCACCT ATCGTCACCA CTCCAGTGAC AACAAGAGGT GGAGGAAGAA GATCATAGAG
AAGCAGCCGC AGAGCCCCAA AGCCCCTGCC CCTCAGCCGC CCCCCATCCT CAAAGTCTTC
AACCGGCCTA TCCTCTTTGA CATCGTGTCC CGGGGCTCCA CTGCTGACCT GGACGGGCTG
CTCCCATTCT TGCTGACCCA CAAGAAACGC CTAACTGATG AGGAGTTTCG AGAGCCATCT
ACGGGGAAGA CCTGCCTGCC CAAGGCCTTG CTGAACCTGA GCAATGGCCG CAACGACACC
ATCCCTGTGC TGCTGGACAT CGCGGAGCGC ACCGGCAACA TGAGGGAGTT CATTAACTCG
CCCTTCCGTG ACATCTACTA TCGAGGTCAG ACAGCCCTGC ACATCGCCAT TGAGCGTCGC
TGCAAACACT ACGTGGAACT TCTCGTGGCC CAGGGAGCTG ATGTCCACGC CCAGGCCCGT
GGGCGCTTCT TCCAGCCCAA GGATGAGGGG GGCTACTTCT ACTTTGGGGA GCTGCCCCTG
TCGCTGGCTG CCTGCACCAA CCAGCCCCAC ATTGTCAACT ACCTGACGGA GAACCCCCAC
AAGAAGGCGG ACATGCGGCG CCAGGACTCG CGAGGCAACA CAGTGCTGCA TGCGCTGGTG
GCCATTGCTG ACAACACCCG TGAGAACACC AAGTTTGTTA CCAAGATGTA CGACCTGCTG
CTGCTCAAGT GTGCCCGCCT CTTCCCCGAC AGCAACCTGG AGGCCGTGCT CAACAACGAC
GGCCTCTCGC CCCTCATGAT GGCTGCCAAG ACGGGCAAGA TTGGGATCTT TCAGCACATC
ATCCGGCGGG AGGTGACGGA TGAGGACACA CGGCACCTGT CCCGCAAGTT CAAGGACTGG
GCCTATGGGC CAGTGTATTC CTCGCTTTAT GACCTCTCCT CCCTGGACAC GTGTGGGGAA
GAGGCCTCCG TGCTGGAGAT CCTGGTGTAC AACAGCAAGA TTGAGAACCG CCACGAGATG
CTGGCTGTGG AGCCCATCAA TGAACTGCTG CGGGACAAGT GGCGCAAGTT CGGGGCCGTC
TCCTTCTACA TCAACGTGGT CTCCTACCTG TGTGCCATGG TCATCTTCAC TCTCACCGCC
TACTACCAGC CGCTGGAGGG CACACCGCCG TACCCTTACC GCACCACGGT GGACTACCTG
CGGCTGGCTG GCGAGGTCAT TACGCTCTTC ACTGGGGTCC TGTTCTTCTT CACCAACATC
AAAGACTTGT TCATGAAGAA ATGCCCTGGA GTGAATTCTC TCTTCATTGA TGGCTCCTTC
CAGCTGCTCT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Role of transient receptor potential and pannexin channels in cigarette smoke-triggered ATP release in the lung
Thorax 69 (12), 1080-1089 (2014)
Baxter M, Eltom S, Dekkak B, Yew-Booth L, Dubuis ED, Maher SA, Belvisi MG and Birrell MA.


book

TRPV4, TRPC1, and TRPP2 assemble to form a flow-sensitive heteromeric channel
FASEB J. 28 (11), 4677-4685 (2014)
Du J, Ma X, Shen B, Huang Y, Birnbaumer L and Yao X.


book

Cathepsin S causes inflammatory pain via biased agonism of PAR2 and TRPV4
J. Biol. Chem. 289 (39), 27215-27234 (2014)
Zhao P, Lieu T, Barlow N, Metcalf M, Veldhuis NA, Jensen DD, Kocan M, Sostegni S, Haerteis S, Baraznenok V, Henderson I, Lindstrom E, Guerrero-Alba R, Valdez-Morales EE, Liedtke W, McIntyre P, Vanner SJ, Korbmacher C and Bunnett NW.


book

Primary cilia signaling mediates intraocular pressure sensation
Proc. Natl. Acad. Sci. U.S.A. 111 (35), 12871-12876 (2014)
Luo N, Conwell MD, Chen X, Kettenhofen CI, Westlake CJ, Cantor LB, Wells CD, Weinreb RN, Corson TW, Spandau DF, Joos KM, Iomini C, Obukhov AG and Sun Y.


book

OTRPC4, a nonselective cation channel that confers sensitivity to extracellular osmolarity
Nat. Cell Biol. 2 (10), 695-702 (2000)
Strotmann R, Harteneck C, Nunnenmacher K, Schultz G and Plant TD.


book

From worm to man: three subfamilies of TRP channels
Trends Neurosci. 23 (4), 159-166 (2000)
Harteneck C, Plant TD and Schultz G.


book

Varying occurrence of vocal cord paralysis in a family with autosomal dominant hereditary motor and sensory neuropathy
J. Neurol. 246 (7), 552-555 (1999)
Donaghy M and Kennett R.


book

TRPV4-Associated Disorders
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Schindler,A., Sumner,C. and Hoover-Fong,J.E.


book

Charcot-Marie-Tooth Hereditary Neuropathy Overview
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Bird,T.D.


book

Charcot-Marie-Tooth Neuropathy Type 2
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Bird,T.D.


 
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