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RCN2 reticulocalbin 2, EF-hand calcium binding domain [Homo sapiens (human)]

Gene Symbol RCN2
Entrez Gene ID 5955
Full Name reticulocalbin 2, EF-hand calcium binding domain
Synonyms E6BP, ERC-55, ERC55, TCBP49
General protein information
Preferred Names
reticulocalbin-2
Names
reticulocalbin-2
E6-binding protein
calcium-binding protein ERC-55
reticulocalbin 2, EF-hand calcium binding domain (endoplasmic reticulum calcium-binding protein, 55kD)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

15

15q23

Summary The protein encoded by this gene is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. This gene maps to the same region as type 4 Bardet-Biedl syndrome, suggesting a possible causative role for this gene in the disorder. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2012]. lac of sum
Disorder MIM:

602584

Disorder Html:





The following RCN2 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RCN2 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu00150 NM_001271837 Homo sapiens reticulocalbin 2, EF-hand calcium binding domain (RCN2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $319
OHu31757 NM_002902 Homo sapiens reticulocalbin 2, EF-hand calcium binding domain (RCN2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $309

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu00150
Accession Version NM_001271837.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1008bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product reticulocalbin-2 isoform b precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB452318.1, BP275421.1, AV711990.1, AK313833.1 and AC051643.5. Summary: The protein encoded by this gene is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. This gene maps to the same region as type 4 Bardet-Biedl syndrome, suggesting a possible causative role for this gene in the disorder. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2012]. Transcript Variant: This variant (2) has an additional in-frame exon in the coding region, compared to variant 1. The resulting isoform (b) is longer than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGCGGCTGG GCCCGAGGAC CGCGGCGTTG GGGCTGCTGC TGCTGTGCGC CGCCGCGGCC 
GGCGCCGGCA AGGCCGAGGA GCTGCACTAC CCGCTGGGCG AGCGCCGCAG CGACTACGAC
CGCGAGGCGC TGCTGGGCGT CCAGGAAGAT GTGGATGAAT ATGTTAAACT CGGCCACGAA
GAGCAGCAAA AAAGACTGCA GGCGATCATA AAGAAAATCG ACTTGGACTC AGATGGCTTT
CTCACTGAAA GTGAACTCAG TTCATGGATT CAGATGTCTT TTAAGCATTA TGCTATGCAA
GAAGCAAAAC AACAGTTTGT TGAATATGAT AAAAACAGTG ATGATACTGT GACTTGGGAT
GAATATAACA TTCAGATGTA TGATCGTGTG ATTGACTTTG ATGAGAACAC TGCTCTGGAT
GATGCAGAAG AGGAGTCCTT TAGGAAGGAA TTTGCCATTT GTAAAAAACA GTCTTTCTGT
TTTTGGCTTC TTCGATTTAA TCTTCACTTA AAGGACAAGA AGCGATTTGA AAAAGCTAAC
CAGGATTCAG GTCCCGGTTT GAGTCTTGAA GAATTTATTG CTTTTGAGCA TCCTGAAGAA
GTTGATTATA TGACGGAATT TGTCATTCAA GAAGCTTTAG AAGAACATGA CAAAAATGGT
GATGGATTTG TTAGTTTGGA AGAATTTCTT GGTGATTACA GGTGGGATCC AACTGCAAAT
GAAGATCCAG AATGGATACT TGTTGAGAAA GACAGATTCG TGAATGATTA TGACAAAGAT
AACGATGGCA GGCTTGATCC CCAAGAGCTG TTACCTTGGG TAGTACCTAA TAATCAGGGC
ATTGCACAAG AGGAGGCGCT TCATCTAATT GATGAAATGG ATTTGAATGG TGACAAAAAG
CTCTCTGAAG AAGAGATTCT GGAAAACCCG GACTTGTTTC TCACCAGTGA AGCCACAGAT
TATGGCAGAC AGCTCCATGA TGACTATTTC TATCATGATG AGCTTTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001258766.1
CDS222..1229
Misc Feature(1)414..584(+)
Misc Feature(2)441..584(+)
Misc Feature(3)846..1043(+)
Misc Feature(4)870..1028(+)
Misc Feature(5)984..1145(+)
Misc Feature(6)990..1145(+)
Misc Feature(7)993..1136(+)
Exon (1)1..365
Gene:RCN2
Gene Synonym:
Exon (2)366..471
Gene:RCN2
Gene Synonym:
Exon (3)472..668
Gene:RCN2
Gene Synonym:
Exon (4)669..722
Gene:RCN2
Gene Synonym:
Exon (5)723..836
Gene:RCN2
Gene Synonym:
Exon (6)837..933
Gene:RCN2
Gene Synonym:
Exon (7)934..1076
Gene:RCN2
Gene Synonym:
Exon (8)1077..2267
Gene:RCN2
Gene Synonym:
Translation
Position Chain Variation Link
7 7 a, c dbSNP:555740558
11 11 a, t dbSNP:573942694
24 24 a, c dbSNP:537648395
40 40 a, g dbSNP:774845630
47 47 c, t dbSNP:556294689
56 56 c, g dbSNP:374769638
58 58 c, g dbSNP:546312014
61 61 a, t dbSNP:564400916
62 62 g, t dbSNP:572946866
65 65 c, t dbSNP:540221073
92 92 c, t dbSNP:759554170
122 122 g, t dbSNP:767643437
147 147 g, t dbSNP:533126822
149 149 a, g dbSNP:529495010
182 182 c, t dbSNP:781504195
184 184 c, g dbSNP:550912225
278 278 a, g dbSNP:756265024
281 281 c, t dbSNP:1567672
300 300 a, g dbSNP:563134720
328 328 c, g dbSNP:780392204
331 331 c, g dbSNP:775367134
353 353 a, g dbSNP:749858758
360 360 a, g dbSNP:533344320
367 367 a, g dbSNP:762930236
380 380 -, at dbSNP:752482231
380 380 a, g dbSNP:557795097
390 390 a, c dbSNP:144252373
393 393 a, g dbSNP:761688264
395 395 c, t dbSNP:368019817
397 397 a, g dbSNP:767610268
398 398 c, g dbSNP:750696653
405 405 -, c dbSNP:760643300
406 406 a, c dbSNP:756142940
407 407 c, g dbSNP:780165153
421 421 a, g dbSNP:763777775
425 425 g, t dbSNP:148834022
434 434 g, t dbSNP:755438411
444 444 c, t dbSNP:779419307
446 446 a, g dbSNP:371622367
476 476 a, g dbSNP:151334127
483 483 c, t dbSNP:778138548
485 485 a, c dbSNP:747335141
486 486 c, t dbSNP:755755482
496 496 g, t dbSNP:779878227
512 512 c, t dbSNP:752058125
513 513 a, g dbSNP:768325166
516 516 a, g dbSNP:774428228
536 536 a, g dbSNP:748176166
554 554 a, g dbSNP:11547510
558 558 a, g dbSNP:373091250
564 564 a, g dbSNP:771917750
565 565 a, g dbSNP:114754379
579 579 c, g dbSNP:760409700
591 591 a, c dbSNP:766564487
597 597 a, t dbSNP:776877755
605 605 g, t dbSNP:759605295
606 606 c, t dbSNP:765356365
607 607 a, g dbSNP:752730439
613 613 -, ttgact dbSNP:763993456
630 630 a, g dbSNP:758870150
631 631 c, g dbSNP:764535405
632 632 c, t dbSNP:751907756
637 637 c, t dbSNP:757748341
648 648 a, g dbSNP:779824870
653 653 a, g dbSNP:749017806
655 655 a, c dbSNP:754662111
660 660 c, t dbSNP:754524655
693 693 g, t dbSNP:773781635
700 700 a, g dbSNP:750832692
714 714 c, t dbSNP:754612421
715 715 a, g dbSNP:556906206
716 716 a, g dbSNP:578238571
720 720 a, g dbSNP:200469558
727 727 a, g dbSNP:763061885
728 728 c, g dbSNP:78925049
740 740 a, g dbSNP:768688268
744 744 c, t dbSNP:774911517
745 745 a, g dbSNP:762353999
765 765 c, g dbSNP:768143673
776 776 c, g, t dbSNP:142859413
777 777 a, g dbSNP:191064420
792 792 g, t dbSNP:752356817
797 797 c, t dbSNP:757910578
801 801 a, g dbSNP:144772621
814 814 c, t dbSNP:751070780
822 822 a, g dbSNP:374950090
829 829 a, g dbSNP:147190935
834 834 a, t dbSNP:745644595
835 835 c, t dbSNP:769520193
836 836 a, g dbSNP:780321268
860 860 a, g dbSNP:377671184
865 865 a, g dbSNP:747999248
884 884 c, t dbSNP:779300135
899 899 a, g dbSNP:577910675
900 900 a, g dbSNP:748216713
921 921 a, g dbSNP:772343896
934 934 c, t dbSNP:777931699
940 940 a, g dbSNP:747140334
948 948 c, g dbSNP:771290377
958 958 c, t dbSNP:781745077
964 964 c, t dbSNP:746184103
971 971 a, g dbSNP:770214433
974 974 a, c dbSNP:773984025
977 977 a, g dbSNP:140517552
980 980 c, t dbSNP:201141990
991 991 -, a dbSNP:776066726
1003 1003 a, g dbSNP:772558525
1004 1004 c, t dbSNP:144470086
1005 1005 a, g dbSNP:766204743
1007 1007 c, t dbSNP:753854459
1024 1024 a, g dbSNP:759391065
1029 1029 c, g dbSNP:765067463
1040 1040 a, g dbSNP:11547509
1048 1048 c, g dbSNP:534252054
1057 1057 a, g dbSNP:758650962
1058 1058 c, g dbSNP:778161456
1061 1061 c, t dbSNP:374423736
1064 1064 a, t dbSNP:556021501
1065 1065 a, g dbSNP:751566644
1078 1078 c, t dbSNP:539579177
1079 1079 a, g dbSNP:17849874
1079 1079 a, g dbSNP:58665121
1081 1081 a, t dbSNP:181742547
1109 1109 c, t dbSNP:140410491
1111 1111 a, g dbSNP:749632542
1112 1112 g, t dbSNP:76782339
1132 1132 a, g dbSNP:755376949
1150 1150 c, t dbSNP:771048379
1151 1151 a, g dbSNP:199624474
1157 1157 g, t dbSNP:770494865
1166 1166 a, c dbSNP:776165141
1174 1174 c, g dbSNP:745392983
1176 1176 a, g dbSNP:769828935
1189 1189 g, t dbSNP:775604128
1190 1190 a, g dbSNP:762832825
1193 1193 g, t dbSNP:776644239
1196 1196 c, g dbSNP:375983396
1199 1199 c, t dbSNP:370868767
1202 1202 c, t dbSNP:769262379
1206 1206 c, t dbSNP:762030069
1208 1208 c, t dbSNP:15939
1212 1212 c, t dbSNP:750454357
1219 1219 a, c dbSNP:756237554
1220 1220 c, t dbSNP:766840449
1224 1224 c, t dbSNP:754410852
1232 1232 c, t dbSNP:755453022
1234 1234 c, g, t dbSNP:1711018
1235 1235 a, g dbSNP:371890911
1237 1237 g, t dbSNP:780753720
1246 1246 a, g dbSNP:745335936
1247 1247 a, g dbSNP:375076612
1251 1251 a, g dbSNP:775552734
1252 1252 a, g dbSNP:368850553
1255 1255 c, t dbSNP:768485583
1262 1262 c, t dbSNP:774160317
1265 1265 a, t dbSNP:761539651
1268 1268 -, a dbSNP:760416825
1277 1277 a, g dbSNP:767757361
1304 1304 g, t dbSNP:76720056
1401 1401 a, g dbSNP:116415216
1402 1402 a, g dbSNP:138335347
1417 1417 c, t dbSNP:1706848
1461 1461 a, g dbSNP:186348430
1481 1481 a, c dbSNP:764689827
1530 1530 c, g dbSNP:752149606
1565 1565 a, g dbSNP:542561711
1573 1573 a, g dbSNP:564384405
1583 1583 a, g dbSNP:531465109
1587 1587 a, g dbSNP:765754860
1608 1608 c, g dbSNP:753397331
1755 1755 c, t dbSNP:543297045
1766 1766 a, g dbSNP:564765831
1840 1840 a, g dbSNP:79159814
1886 1886 c, t dbSNP:551623311
1914 1914 -, tgtt dbSNP:35334964
1920 1920 -, tttg dbSNP:76311305
1968 1968 a, t dbSNP:566910732
1975 1975 a, t dbSNP:527707748
1978 1978 c, t dbSNP:549325285
1979 1979 -, g dbSNP:573996156
2055 2055 -, t dbSNP:577533640
2088 2088 a, g dbSNP:754534224
2099 2099 c, t dbSNP:778688215
2157 2157 -, c dbSNP:112938628
2164 2164 c, t dbSNP:567649887
2209 2209 g, t dbSNP:538569442
2230 2230 a, g dbSNP:764722513
2245 2245 a, c dbSNP:752432106

Target ORF information:

RefSeq Version NM_001271837
Organism Homo sapiens (human)
Definition Homo sapiens reticulocalbin 2, EF-hand calcium binding domain (RCN2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001271837

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGCGGCTGG GCCCGAGGAC CGCGGCGTTG GGGCTGCTGC TGCTGTGCGC CGCCGCGGCC 
GGCGCCGGCA AGGCCGAGGA GCTGCACTAC CCGCTGGGCG AGCGCCGCAG CGACTACGAC
CGCGAGGCGC TGCTGGGCGT CCAGGAAGAT GTGGATGAAT ATGTTAAACT CGGCCACGAA
GAGCAGCAAA AAAGACTGCA GGCGATCATA AAGAAAATCG ACTTGGACTC AGATGGCTTT
CTCACTGAAA GTGAACTCAG TTCATGGATT CAGATGTCTT TTAAGCATTA TGCTATGCAA
GAAGCAAAAC AACAGTTTGT TGAATATGAT AAAAACAGTG ATGATACTGT GACTTGGGAT
GAATATAACA TTCAGATGTA TGATCGTGTG ATTGACTTTG ATGAGAACAC TGCTCTGGAT
GATGCAGAAG AGGAGTCCTT TAGGAAGGAA TTTGCCATTT GTAAAAAACA GTCTTTCTGT
TTTTGGCTTC TTCGATTTAA TCTTCACTTA AAGGACAAGA AGCGATTTGA AAAAGCTAAC
CAGGATTCAG GTCCCGGTTT GAGTCTTGAA GAATTTATTG CTTTTGAGCA TCCTGAAGAA
GTTGATTATA TGACGGAATT TGTCATTCAA GAAGCTTTAG AAGAACATGA CAAAAATGGT
GATGGATTTG TTAGTTTGGA AGAATTTCTT GGTGATTACA GGTGGGATCC AACTGCAAAT
GAAGATCCAG AATGGATACT TGTTGAGAAA GACAGATTCG TGAATGATTA TGACAAAGAT
AACGATGGCA GGCTTGATCC CCAAGAGCTG TTACCTTGGG TAGTACCTAA TAATCAGGGC
ATTGCACAAG AGGAGGCGCT TCATCTAATT GATGAAATGG ATTTGAATGG TGACAAAAAG
CTCTCTGAAG AAGAGATTCT GGAAAACCCG GACTTGTTTC TCACCAGTGA AGCCACAGAT
TATGGCAGAC AGCTCCATGA TGACTATTTC TATCATGATG AGCTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu31757
Accession Version NM_002902.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product reticulocalbin-2 isoform a precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB452318.1, AK313833.1 and AC051643.5. On Feb 10, 2009 this sequence version replaced gi:4506456. Summary: The protein encoded by this gene is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. This gene maps to the same region as type 4 Bardet-Biedl syndrome, suggesting a possible causative role for this gene in the disorder. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2012]. Transcript Variant: This variant (1) is a predominant transcript and encodes isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: X78669.1, BC004892.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCGGCTGG GCCCGAGGAC CGCGGCGTTG GGGCTGCTGC TGCTGTGCGC CGCCGCGGCC 
GGCGCCGGCA AGGCCGAGGA GCTGCACTAC CCGCTGGGCG AGCGCCGCAG CGACTACGAC
CGCGAGGCGC TGCTGGGCGT CCAGGAAGAT GTGGATGAAT ATGTTAAACT CGGCCACGAA
GAGCAGCAAA AAAGACTGCA GGCGATCATA AAGAAAATCG ACTTGGACTC AGATGGCTTT
CTCACTGAAA GTGAACTCAG TTCATGGATT CAGATGTCTT TTAAGCATTA TGCTATGCAA
GAAGCAAAAC AACAGTTTGT TGAATATGAT AAAAACAGTG ATGATACTGT GACTTGGGAT
GAATATAACA TTCAGATGTA TGATCGTGTG ATTGACTTTG ATGAGAACAC TGCTCTGGAT
GATGCAGAAG AGGAGTCCTT TAGGAAGCTT CACTTAAAGG ACAAGAAGCG ATTTGAAAAA
GCTAACCAGG ATTCAGGTCC CGGTTTGAGT CTTGAAGAAT TTATTGCTTT TGAGCATCCT
GAAGAAGTTG ATTATATGAC GGAATTTGTC ATTCAAGAAG CTTTAGAAGA ACATGACAAA
AATGGTGATG GATTTGTTAG TTTGGAAGAA TTTCTTGGTG ATTACAGGTG GGATCCAACT
GCAAATGAAG ATCCAGAATG GATACTTGTT GAGAAAGACA GATTCGTGAA TGATTATGAC
AAAGATAACG ATGGCAGGCT TGATCCCCAA GAGCTGTTAC CTTGGGTAGT ACCTAATAAT
CAGGGCATTG CACAAGAGGA GGCGCTTCAT CTAATTGATG AAATGGATTT GAATGGTGAC
AAAAAGCTCT CTGAAGAAGA GATTCTGGAA AACCCGGACT TGTTTCTCAC CAGTGAAGCC
ACAGATTATG GCAGACAGCT CCATGATGAC TATTTCTATC ATGATGAGCT TTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_002893.1
CDS222..1175
Misc Feature(1)414..584(+)
Misc Feature(2)441..584(+)
Misc Feature(3)792..989(+)
Misc Feature(4)816..974(+)
Misc Feature(5)930..1091(+)
Misc Feature(6)936..1091(+)
Misc Feature(7)939..1082(+)
Misc Feature(8)1161..1172(+)
Exon (1)1..365
Gene:RCN2
Gene Synonym:
Exon (2)366..471
Gene:RCN2
Gene Synonym:
Exon (3)472..668
Gene:RCN2
Gene Synonym:
Exon (4)669..782
Gene:RCN2
Gene Synonym:
Exon (5)783..879
Gene:RCN2
Gene Synonym:
Exon (6)880..1022
Gene:RCN2
Gene Synonym:
Exon (7)1023..2213
Gene:RCN2
Gene Synonym:
Translation
Position Chain Variation Link
7 7 a, c dbSNP:555740558
11 11 a, t dbSNP:573942694
24 24 a, c dbSNP:537648395
40 40 a, g dbSNP:774845630
47 47 c, t dbSNP:556294689
56 56 c, g dbSNP:374769638
58 58 c, g dbSNP:546312014
61 61 a, t dbSNP:564400916
62 62 g, t dbSNP:572946866
65 65 c, t dbSNP:540221073
92 92 c, t dbSNP:759554170
122 122 g, t dbSNP:767643437
147 147 g, t dbSNP:533126822
149 149 a, g dbSNP:529495010
182 182 c, t dbSNP:781504195
184 184 c, g dbSNP:550912225
278 278 a, g dbSNP:756265024
281 281 c, t dbSNP:1567672
300 300 a, g dbSNP:563134720
328 328 c, g dbSNP:780392204
331 331 c, g dbSNP:775367134
353 353 a, g dbSNP:749858758
360 360 a, g dbSNP:533344320
367 367 a, g dbSNP:762930236
380 380 -, at dbSNP:752482231
380 380 a, g dbSNP:557795097
390 390 a, c dbSNP:144252373
393 393 a, g dbSNP:761688264
395 395 c, t dbSNP:368019817
397 397 a, g dbSNP:767610268
398 398 c, g dbSNP:750696653
405 405 -, c dbSNP:760643300
406 406 a, c dbSNP:756142940
407 407 c, g dbSNP:780165153
421 421 a, g dbSNP:763777775
425 425 g, t dbSNP:148834022
434 434 g, t dbSNP:755438411
444 444 c, t dbSNP:779419307
446 446 a, g dbSNP:371622367
476 476 a, g dbSNP:151334127
483 483 c, t dbSNP:778138548
485 485 a, c dbSNP:747335141
486 486 c, t dbSNP:755755482
496 496 g, t dbSNP:779878227
512 512 c, t dbSNP:752058125
513 513 a, g dbSNP:768325166
516 516 a, g dbSNP:774428228
536 536 a, g dbSNP:748176166
554 554 a, g dbSNP:11547510
558 558 a, g dbSNP:373091250
564 564 a, g dbSNP:771917750
565 565 a, g dbSNP:114754379
579 579 c, g dbSNP:760409700
591 591 a, c dbSNP:766564487
597 597 a, t dbSNP:776877755
605 605 g, t dbSNP:759605295
606 606 c, t dbSNP:765356365
607 607 a, g dbSNP:752730439
613 613 -, ttgact dbSNP:763993456
630 630 a, g dbSNP:758870150
631 631 c, g dbSNP:764535405
632 632 c, t dbSNP:751907756
637 637 c, t dbSNP:757748341
648 648 a, g dbSNP:779824870
653 653 a, g dbSNP:749017806
655 655 a, c dbSNP:754662111
660 660 c, t dbSNP:754524655
673 673 a, g dbSNP:763061885
674 674 c, g dbSNP:78925049
686 686 a, g dbSNP:768688268
690 690 c, t dbSNP:774911517
691 691 a, g dbSNP:762353999
711 711 c, g dbSNP:768143673
722 722 c, g, t dbSNP:142859413
723 723 a, g dbSNP:191064420
738 738 g, t dbSNP:752356817
743 743 c, t dbSNP:757910578
747 747 a, g dbSNP:144772621
760 760 c, t dbSNP:751070780
768 768 a, g dbSNP:374950090
775 775 a, g dbSNP:147190935
780 780 a, t dbSNP:745644595
781 781 c, t dbSNP:769520193
782 782 a, g dbSNP:780321268
806 806 a, g dbSNP:377671184
811 811 a, g dbSNP:747999248
830 830 c, t dbSNP:779300135
845 845 a, g dbSNP:577910675
846 846 a, g dbSNP:748216713
867 867 a, g dbSNP:772343896
880 880 c, t dbSNP:777931699
886 886 a, g dbSNP:747140334
894 894 c, g dbSNP:771290377
904 904 c, t dbSNP:781745077
910 910 c, t dbSNP:746184103
917 917 a, g dbSNP:770214433
920 920 a, c dbSNP:773984025
923 923 a, g dbSNP:140517552
926 926 c, t dbSNP:201141990
937 937 -, a dbSNP:776066726
949 949 a, g dbSNP:772558525
950 950 c, t dbSNP:144470086
951 951 a, g dbSNP:766204743
953 953 c, t dbSNP:753854459
970 970 a, g dbSNP:759391065
975 975 c, g dbSNP:765067463
986 986 a, g dbSNP:11547509
994 994 c, g dbSNP:534252054
1003 1003 a, g dbSNP:758650962
1004 1004 c, g dbSNP:778161456
1007 1007 c, t dbSNP:374423736
1010 1010 a, t dbSNP:556021501
1011 1011 a, g dbSNP:751566644
1024 1024 c, t dbSNP:539579177
1025 1025 a, g dbSNP:17849874
1025 1025 a, g dbSNP:58665121
1027 1027 a, t dbSNP:181742547
1055 1055 c, t dbSNP:140410491
1057 1057 a, g dbSNP:749632542
1058 1058 g, t dbSNP:76782339
1078 1078 a, g dbSNP:755376949
1096 1096 c, t dbSNP:771048379
1097 1097 a, g dbSNP:199624474
1103 1103 g, t dbSNP:770494865
1112 1112 a, c dbSNP:776165141
1120 1120 c, g dbSNP:745392983
1122 1122 a, g dbSNP:769828935
1135 1135 g, t dbSNP:775604128
1136 1136 a, g dbSNP:762832825
1139 1139 g, t dbSNP:776644239
1142 1142 c, g dbSNP:375983396
1145 1145 c, t dbSNP:370868767
1148 1148 c, t dbSNP:769262379
1152 1152 c, t dbSNP:762030069
1154 1154 c, t dbSNP:15939
1158 1158 c, t dbSNP:750454357
1165 1165 a, c dbSNP:756237554
1166 1166 c, t dbSNP:766840449
1170 1170 c, t dbSNP:754410852
1178 1178 c, t dbSNP:755453022
1180 1180 c, g, t dbSNP:1711018
1181 1181 a, g dbSNP:371890911
1183 1183 g, t dbSNP:780753720
1192 1192 a, g dbSNP:745335936
1193 1193 a, g dbSNP:375076612
1197 1197 a, g dbSNP:775552734
1198 1198 a, g dbSNP:368850553
1201 1201 c, t dbSNP:768485583
1208 1208 c, t dbSNP:774160317
1211 1211 a, t dbSNP:761539651
1214 1214 -, a dbSNP:760416825
1223 1223 a, g dbSNP:767757361
1250 1250 g, t dbSNP:76720056
1347 1347 a, g dbSNP:116415216
1348 1348 a, g dbSNP:138335347
1363 1363 c, t dbSNP:1706848
1407 1407 a, g dbSNP:186348430
1427 1427 a, c dbSNP:764689827
1476 1476 c, g dbSNP:752149606
1511 1511 a, g dbSNP:542561711
1519 1519 a, g dbSNP:564384405
1529 1529 a, g dbSNP:531465109
1533 1533 a, g dbSNP:765754860
1554 1554 c, g dbSNP:753397331
1701 1701 c, t dbSNP:543297045
1712 1712 a, g dbSNP:564765831
1786 1786 a, g dbSNP:79159814
1832 1832 c, t dbSNP:551623311
1860 1860 -, tgtt dbSNP:35334964
1866 1866 -, tttg dbSNP:76311305
1914 1914 a, t dbSNP:566910732
1921 1921 a, t dbSNP:527707748
1924 1924 c, t dbSNP:549325285
1925 1925 -, g dbSNP:573996156
2001 2001 -, t dbSNP:577533640
2034 2034 a, g dbSNP:754534224
2045 2045 c, t dbSNP:778688215
2103 2103 -, c dbSNP:112938628
2110 2110 c, t dbSNP:567649887
2155 2155 g, t dbSNP:538569442
2176 2176 a, g dbSNP:764722513
2191 2191 a, c dbSNP:752432106

Target ORF information:

RefSeq Version NM_002902
Organism Homo sapiens (human)
Definition Homo sapiens reticulocalbin 2, EF-hand calcium binding domain (RCN2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002902

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCGGCTGG GCCCGAGGAC CGCGGCGTTG GGGCTGCTGC TGCTGTGCGC CGCCGCGGCC 
GGCGCCGGCA AGGCCGAGGA GCTGCACTAC CCGCTGGGCG AGCGCCGCAG CGACTACGAC
CGCGAGGCGC TGCTGGGCGT CCAGGAAGAT GTGGATGAAT ATGTTAAACT CGGCCACGAA
GAGCAGCAAA AAAGACTGCA GGCGATCATA AAGAAAATCG ACTTGGACTC AGATGGCTTT
CTCACTGAAA GTGAACTCAG TTCATGGATT CAGATGTCTT TTAAGCATTA TGCTATGCAA
GAAGCAAAAC AACAGTTTGT TGAATATGAT AAAAACAGTG ATGATACTGT GACTTGGGAT
GAATATAACA TTCAGATGTA TGATCGTGTG ATTGACTTTG ATGAGAACAC TGCTCTGGAT
GATGCAGAAG AGGAGTCCTT TAGGAAGCTT CACTTAAAGG ACAAGAAGCG ATTTGAAAAA
GCTAACCAGG ATTCAGGTCC CGGTTTGAGT CTTGAAGAAT TTATTGCTTT TGAGCATCCT
GAAGAAGTTG ATTATATGAC GGAATTTGTC ATTCAAGAAG CTTTAGAAGA ACATGACAAA
AATGGTGATG GATTTGTTAG TTTGGAAGAA TTTCTTGGTG ATTACAGGTG GGATCCAACT
GCAAATGAAG ATCCAGAATG GATACTTGTT GAGAAAGACA GATTCGTGAA TGATTATGAC
AAAGATAACG ATGGCAGGCT TGATCCCCAA GAGCTGTTAC CTTGGGTAGT ACCTAATAAT
CAGGGCATTG CACAAGAGGA GGCGCTTCAT CTAATTGATG AAATGGATTT GAATGGTGAC
AAAAAGCTCT CTGAAGAAGA GATTCTGGAA AACCCGGACT TGTTTCTCAC CAGTGAAGCC
ACAGATTATG GCAGACAGCT CCATGATGAC TATTTCTATC ATGATGAGCT TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.