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BCL2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol BCL2
Entrez Gene ID 596
Full Name B-cell CLL/lymphoma 2
Synonyms Bcl-2, PPP1R50
General protein information
Preferred Names
apoptosis regulator Bcl-2
Names
apoptosis regulator Bcl-2
protein phosphatase 1, regulatory subunit 50
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

18

18q21.3

Summary This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

151430

Disorder Html: Leukemia/lymphoma, B-cell, 2 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011526135 XP_011524437 apoptosis regulator Bcl-2 isoform X1
NM_000633 NP_000624 apoptosis regulator Bcl-2 alpha isoform
NM_000657 NP_000648 apoptosis regulator Bcl-2 beta isoform



Homo sapiens (human) BCL2 NP_000624.2
Pan troglodytes (chimpanzee) BCL2 XP_001145537.1
Canis lupus familiaris (dog) BCL2 NP_001002949.1
Bos taurus (cattle) BCL2 NP_001159958.1
Mus musculus (house mouse) Bcl2 NP_033871.2
Rattus norvegicus (Norway rat) Bcl2 NP_058689.1
Gallus gallus (chicken) BCL2 NP_990670.1
Xenopus (Silurana) tropicalis (western clawed frog) bcl2 XP_002934442.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BCL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BCL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu51275 XM_011526135 PREDICTED: Homo sapiens B-cell CLL/lymphoma 2 (BCL2), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $139.30
$199.00
OHu25505
NM_000633 Homo sapiens B-cell CLL/lymphoma 2 (BCL2), transcript variant alpha, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu25321 NM_000657 Homo sapiens B-cell CLL/lymphoma 2 (BCL2), transcript variant beta, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu51275
Clone ID Related Accession (Same CDS sequence) XM_011526135
Accession Version XM_011526135.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 675bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product apoptosis regulator Bcl-2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010966.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCGCACG CTGGGAGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAT 
TATAAGCTGT CGCAGAGGGG CTACGAGTGG GATGCGGGAG ATGTGGGCGC CGCGCCCCCG
GGGGCCGCCC CCGCACCGGG CATCTTCTCC TCCCAGCCCG GGCACACGCC CCATCCAGCC
GCATCCCGGG ACCCGGTCGC CAGGACCTCG CCGCTGCAGA CCCCGGCTGC CCCCGGCGCC
GCCGCGGGGC CTGCGCTCAG CCCGGTGCCA CCTGTGGTCC ACCTGACCCT CCGCCAGGCC
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC
CTGACGCCCT TCACCGCGCG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAC
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC
CTGAACCGGC ACCTGCACAC CTGGATCCAG GATAACGGAG GCTGGGATGC CCTTGTGTTA
TTTGCAGGGC TAAGGGAGTT TCAGCATGGG CCAGGAAACT ACTTTCTTAA AAGAAACTTA
AACGCGAAAT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011524437.1
CDS1113..1787
Misc Feature(1)1113..1721(+)
Misc Feature(2)1131..1211(+)
Misc Feature(3)1368..1715(+)
Misc Feature(4)1401..1427(+)
Misc Feature(5)1410..1715(+)
Misc Feature(6)1518..1574(+)
Misc Feature(7)1671..1715(+)
Translation

Target ORF information:

RefSeq Version XM_011526135
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens B-cell CLL/lymphoma 2 (BCL2), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011526135

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCGCACG CTGGGAGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAT 
TATAAGCTGT CGCAGAGGGG CTACGAGTGG GATGCGGGAG ATGTGGGCGC CGCGCCCCCG
GGGGCCGCCC CCGCACCGGG CATCTTCTCC TCCCAGCCCG GGCACACGCC CCATCCAGCC
GCATCCCGGG ACCCGGTCGC CAGGACCTCG CCGCTGCAGA CCCCGGCTGC CCCCGGCGCC
GCCGCGGGGC CTGCGCTCAG CCCGGTGCCA CCTGTGGTCC ACCTGACCCT CCGCCAGGCC
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC
CTGACGCCCT TCACCGCGCG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAC
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC
CTGAACCGGC ACCTGCACAC CTGGATCCAG GATAACGGAG GCTGGGATGC CCTTGTGTTA
TTTGCAGGGC TAAGGGAGTT TCAGCATGGG CCAGGAAACT ACTTTCTTAA AAGAAACTTA
AACGCGAAAT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25505
Clone ID Related Accession (Same CDS sequence) NM_000633
Accession Version NM_000633.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 720bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product apoptosis regulator Bcl-2 alpha isoform
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC021803.8, BC027258.1 and AC022726.13. This sequence is a reference standard in the RefSeqGene project. On Aug 10, 2005 this sequence version replaced gi:4557354. Transcript Variant: This variant (alpha) represents the longer transcript and encodes the longer isoform (alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta regulatory uORF :: PMID: 9645350 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC027258.1, X06487.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962337, SAMEA962343 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCGCACG CTGGGAGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAT 
TATAAGCTGT CGCAGAGGGG CTACGAGTGG GATGCGGGAG ATGTGGGCGC CGCGCCCCCG
GGGGCCGCCC CCGCACCGGG CATCTTCTCC TCCCAGCCCG GGCACACGCC CCATCCAGCC
GCATCCCGGG ACCCGGTCGC CAGGACCTCG CCGCTGCAGA CCCCGGCTGC CCCCGGCGCC
GCCGCGGGGC CTGCGCTCAG CCCGGTGCCA CCTGTGGTCC ACCTGACCCT CCGCCAGGCC
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC
CTGACGCCCT TCACCGCGCG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAC
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC
CTGAACCGGC ACCTGCACAC CTGGATCCAG GATAACGGAG GCTGGGATGC CTTTGTGGAA
CTGTACGGCC CCAGCATGCG GCCTCTGTTT GATTTCTCCT GGCTGTCTCT GAAGACTCTG
CTCAGTTTGG CCCTGGTGGG AGCTTGCATC ACCCTGGGTG CCTATCTGGG CCACAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000624.2
CDS494..1213
Misc Feature(1)375..410(+)
Misc Feature(2)392..394(+)
Misc Feature(3)494..1210(+)
Misc Feature(4)512..592(+)
Misc Feature(5)521..583(+)
Misc Feature(6)563..565(+)
Misc Feature(7)593..598(+)
Misc Feature(8)593..595(+)
Misc Feature(9)659..661(+)
Misc Feature(10)698..700(+)
Misc Feature(11)698..700(+)
Misc Feature(12)701..703(+)
Misc Feature(13)701..703(+)
Misc Feature(14)701..703(+)
Misc Feature(15)713..715(+)
Misc Feature(16)749..1096(+)
Misc Feature(17)752..754(+)
Misc Feature(18)752..754(+)
Misc Feature(19)752..754(+)
Misc Feature(20)770..814(+)
Misc Feature(21)782..808(+)
Misc Feature(22)791..1096(+)
Misc Feature(23)899..958(+)
Misc Feature(24)899..955(+)
Misc Feature(25)1052..1099(+)
Misc Feature(26)1052..1096(+)
Misc Feature(27)1127..1192(+)
Exon (1)1..207
Gene:BCL2
Gene Synonym:
Exon (2)208..1078
Gene:BCL2
Gene Synonym:
Exon (3)1079..6492
Gene:BCL2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000633
Organism Homo sapiens (human)
Definition Homo sapiens B-cell CLL/lymphoma 2 (BCL2), transcript variant alpha, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000633

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCGCACG CTGGGAGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAT 
TATAAGCTGT CGCAGAGGGG CTACGAGTGG GATGCGGGAG ATGTGGGCGC CGCGCCCCCG
GGGGCCGCCC CCGCACCGGG CATCTTCTCC TCCCAGCCCG GGCACACGCC CCATCCAGCC
GCATCCCGGG ACCCGGTCGC CAGGACCTCG CCGCTGCAGA CCCCGGCTGC CCCCGGCGCC
GCCGCGGGGC CTGCGCTCAG CCCGGTGCCA CCTGTGGTCC ACCTGACCCT CCGCCAGGCC
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC
CTGACGCCCT TCACCGCGCG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAC
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC
CTGAACCGGC ACCTGCACAC CTGGATCCAG GATAACGGAG GCTGGGATGC CTTTGTGGAA
CTGTACGGCC CCAGCATGCG GCCTCTGTTT GATTTCTCCT GGCTGTCTCT GAAGACTCTG
CTCAGTTTGG CCCTGGTGGG AGCTTGCATC ACCCTGGGTG CCTATCTGGG CCACAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25321
Clone ID Related Accession (Same CDS sequence) NM_000657
Accession Version NM_000657.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 618bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product apoptosis regulator Bcl-2 beta isoform
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC021803.8 and AI401297.1. On Aug 10, 2005 this sequence version replaced gi:4557356. Transcript Variant: This variant (beta) differs in the 3' UTR and coding region compared to variant alpha. The resulting isoform (beta) is shorter and has a distinct C-terminus compared to isoform alpha. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta regulatory uORF :: PMID: 9645350 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BQ056709.1, BG258362.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962337, SAMEA962343 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGGCGCACG CTGGGAGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAT 
TATAAGCTGT CGCAGAGGGG CTACGAGTGG GATGCGGGAG ATGTGGGCGC CGCGCCCCCG
GGGGCCGCCC CCGCACCGGG CATCTTCTCC TCCCAGCCCG GGCACACGCC CCATCCAGCC
GCATCCCGGG ACCCGGTCGC CAGGACCTCG CCGCTGCAGA CCCCGGCTGC CCCCGGCGCC
GCCGCGGGGC CTGCGCTCAG CCCGGTGCCA CCTGTGGTCC ACCTGACCCT CCGCCAGGCC
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC
CTGACGCCCT TCACCGCGCG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAC
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC
CTGAACCGGC ACCTGCACAC CTGGATCCAG GATAACGGAG GCTGGGTAGG TGCACTTGGT
GATGTGAGTC TGGGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000648.2
CDS494..1111
Misc Feature(1)375..410(+)
Misc Feature(2)392..394(+)
Misc Feature(3)494..>1078(+)
Misc Feature(4)512..592(+)
Misc Feature(5)521..583(+)
Misc Feature(6)563..565(+)
Misc Feature(7)593..598(+)
Misc Feature(8)593..595(+)
Misc Feature(9)659..661(+)
Misc Feature(10)698..700(+)
Misc Feature(11)698..700(+)
Misc Feature(12)701..703(+)
Misc Feature(13)701..703(+)
Misc Feature(14)713..715(+)
Misc Feature(15)749..1084(+)
Misc Feature(16)752..754(+)
Misc Feature(17)752..754(+)
Misc Feature(18)752..754(+)
Misc Feature(19)752..754(+)
Misc Feature(20)770..814(+)
Misc Feature(21)782..808(+)
Misc Feature(22)791..964(+)
Misc Feature(23)899..958(+)
Misc Feature(24)899..955(+)
Misc Feature(25)1052..1084(+)
Exon (1)1..207
Gene:BCL2
Gene Synonym:
Exon (2)208..1206
Gene:BCL2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000657
Organism Homo sapiens (human)
Definition Homo sapiens B-cell CLL/lymphoma 2 (BCL2), transcript variant beta, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000657

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCGCACG CTGGGAGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAT 
TATAAGCTGT CGCAGAGGGG CTACGAGTGG GATGCGGGAG ATGTGGGCGC CGCGCCCCCG
GGGGCCGCCC CCGCACCGGG CATCTTCTCC TCCCAGCCCG GGCACACGCC CCATCCAGCC
GCATCCCGGG ACCCGGTCGC CAGGACCTCG CCGCTGCAGA CCCCGGCTGC CCCCGGCGCC
GCCGCGGGGC CTGCGCTCAG CCCGGTGCCA CCTGTGGTCC ACCTGACCCT CCGCCAGGCC
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC
CTGACGCCCT TCACCGCGCG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAC
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC
CTGAACCGGC ACCTGCACAC CTGGATCCAG GATAACGGAG GCTGGGTAGG TGCACTTGGT
GATGTGAGTC TGGGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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