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BCL2 B-cell CLL/lymphoma 2 [Homo sapiens (human)]

Gene Symbol BCL2
Entrez Gene ID 596
Full Name B-cell CLL/lymphoma 2
Synonyms Bcl-2, PPP1R50
General protein information
Preferred Names
apoptosis regulator Bcl-2
Names
apoptosis regulator Bcl-2
protein phosphatase 1, regulatory subunit 50
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

18

18q21.3

Summary This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

151430

Disorder Html: Leukemia/lymphoma, B-cell, 2 (3)





The following BCL2 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BCL2 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu51275 XM_011526135 PREDICTED: Homo sapiens B-cell CLL/lymphoma 2 (BCL2), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $199
OHu25505 NM_000633 Homo sapiens B-cell CLL/lymphoma 2 (BCL2), transcript variant alpha, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$269
OHu25321 NM_000657 Homo sapiens B-cell CLL/lymphoma 2 (BCL2), transcript variant beta, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $269

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu51275
Accession Version XM_011526135.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 675bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product apoptosis regulator Bcl-2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010966.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCGCACG CTGGGAGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAT 
TATAAGCTGT CGCAGAGGGG CTACGAGTGG GATGCGGGAG ATGTGGGCGC CGCGCCCCCG
GGGGCCGCCC CCGCACCGGG CATCTTCTCC TCCCAGCCCG GGCACACGCC CCATCCAGCC
GCATCCCGGG ACCCGGTCGC CAGGACCTCG CCGCTGCAGA CCCCGGCTGC CCCCGGCGCC
GCCGCGGGGC CTGCGCTCAG CCCGGTGCCA CCTGTGGTCC ACCTGACCCT CCGCCAGGCC
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC
CTGACGCCCT TCACCGCGCG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAC
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC
CTGAACCGGC ACCTGCACAC CTGGATCCAG GATAACGGAG GCTGGGATGC CCTTGTGTTA
TTTGCAGGGC TAAGGGAGTT TCAGCATGGG CCAGGAAACT ACTTTCTTAA AAGAAACTTA
AACGCGAAAT TCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011524437.1
CDS1113..1787
Misc Feature(1)1113..1721(+)
Misc Feature(2)1131..1211(+)
Misc Feature(3)1368..1715(+)
Misc Feature(4)1401..1427(+)
Misc Feature(5)1410..1715(+)
Misc Feature(6)1518..1574(+)
Misc Feature(7)1671..1715(+)
Translation
Position Chain Variation Link
61 61 a, g dbSNP:751718993
82 82 a, g dbSNP:574045431
102 102 a, g dbSNP:557378066
139 139 c, g dbSNP:764236005
159 159 c, g dbSNP:746970168
175 175 a, c dbSNP:2279115
210 210 g, t dbSNP:747107129
239 239 c, t dbSNP:571580090
255 255 a, c dbSNP:746016409
275 275 a, g dbSNP:551383147
326 326 a, t dbSNP:538522646
411 411 a, g dbSNP:566462049
416 416 -, a dbSNP:34360708
417 417 c, t dbSNP:779249610
463 463 c, g dbSNP:1473418
469 469 c, g dbSNP:192114822
476 476 c, g dbSNP:560936021
521 521 g, t dbSNP:34223591
524 524 g, t dbSNP:550615446
574 574 a, g dbSNP:530542966
600 600 -, a dbSNP:757809447
647 647 a, g dbSNP:4987706
702 702 a, g dbSNP:545059985
740 740 a, g dbSNP:4987707
772 772 a, g dbSNP:769699373
779 779 c, t dbSNP:560134104
799 799 c, t dbSNP:756643057
825 825 a, g dbSNP:189636103
969 969 a, g dbSNP:772250802
970 970 c, t dbSNP:765740867
980 980 c, t dbSNP:55805819
1044 1044 c, t dbSNP:552075974
1064 1064 a, g dbSNP:780722376
1071 1071 c, t dbSNP:114373512
1078 1078 c, t dbSNP:764979303
1085 1085 c, g dbSNP:751238437
1087 1087 c, t dbSNP:778057839
1096 1096 -, c dbSNP:769934430
1099 1099 g, t dbSNP:758699052
1102 1102 c, g dbSNP:753168582
1104 1104 c, t dbSNP:765644873
1105 1105 c, t dbSNP:760135041
1112 1112 a, g dbSNP:193014588
1118 1118 g, t dbSNP:766594821
1120 1120 a, g dbSNP:557714365
1121 1121 c, t dbSNP:773580172
1122 1122 g, t dbSNP:768498963
1133 1133 a, g dbSNP:1801018
1135 1135 a, g dbSNP:775404824
1144 1144 a, g dbSNP:769588208
1155 1155 c, g dbSNP:745851862
1160 1160 a, g dbSNP:776360417
1161 1161 a, g dbSNP:780634396
1163 1163 a, g dbSNP:565741123
1167 1167 a, c dbSNP:746600883
1170 1170 a, c dbSNP:777401949
1172 1172 g, t dbSNP:758123306
1184 1184 g, t dbSNP:752978070
1191 1191 a, g dbSNP:779372254
1196 1196 c, t dbSNP:755397980
1202 1202 a, g dbSNP:754418219
1203 1203 c, g dbSNP:766508625
1206 1206 c, g dbSNP:760840889
1207 1207 c, g dbSNP:546449806
1209 1209 a, g dbSNP:767612613
1211 1211 -, a dbSNP:759748900
1212 1212 c, g, t dbSNP:540701354
1214 1214 c, t dbSNP:762137430
1215 1215 a, g dbSNP:775108608
1223 1223 a, c dbSNP:769656259
1229 1229 a, c, t dbSNP:776558326
1231 1231 c, t dbSNP:770925898
1232 1232 a, g dbSNP:746504624
1233 1233 a, g dbSNP:777309369
1239 1239 a, g dbSNP:1800477
1244 1244 a, c dbSNP:747863570
1245 1245 g, t dbSNP:779096658
1246 1246 c, g dbSNP:755382644
1248 1248 c, g dbSNP:749745319
1249 1249 c, t dbSNP:369277721
1250 1250 a, g dbSNP:756734795
1251 1251 a, g dbSNP:368344129
1256 1256 c, t dbSNP:767668991
1259 1259 c, t dbSNP:200972039
1265 1265 c, t dbSNP:751822316
1271 1271 c, t dbSNP:764877973
1275 1275 c, t dbSNP:759325300
1277 1277 c, t dbSNP:776473075
1279 1279 c, g dbSNP:766277782
1293 1293 a, g, t dbSNP:772554403
1297 1297 a, c, t dbSNP:747696230
1299 1299 c, t dbSNP:774056251
1305 1305 c, t dbSNP:768259110
1308 1308 a, g dbSNP:749649089
1311 1311 a, g dbSNP:780606244
1312 1312 c, t dbSNP:756650851
1314 1314 a, g dbSNP:746401408
1316 1316 a, g dbSNP:781072794
1322 1322 a, g dbSNP:770596713
1325 1325 a, g dbSNP:376115863
1336 1336 c, t dbSNP:757360142
1337 1337 a, g, t dbSNP:764359199
1343 1343 c, t dbSNP:758673021
1344 1344 c, t dbSNP:753603356
1347 1347 c, g dbSNP:766196038
1351 1351 c, t dbSNP:760559670
1357 1357 c, t dbSNP:201827119
1360 1360 a, g dbSNP:375594294
1361 1361 a, g dbSNP:766872577
1362 1362 c, t dbSNP:761382113
1364 1364 g, t dbSNP:773791165
1370 1370 c, t dbSNP:768392602
1371 1371 a, g dbSNP:748978916
1373 1373 c, g, t dbSNP:770293857
1375 1375 c, g, t dbSNP:781718615
1385 1385 c, t dbSNP:771396277
1390 1390 c, t dbSNP:565014924
1391 1391 c, g dbSNP:747085111
1393 1393 a, c dbSNP:551395951
1395 1395 c, g, t dbSNP:373889054
1398 1398 a, g dbSNP:752942153
1400 1400 c, g dbSNP:557922819
1402 1402 c, t dbSNP:528042823
1405 1405 g, t dbSNP:755908754
1408 1408 a, g dbSNP:559397524
1411 1411 c, t dbSNP:750253286
1412 1412 c, t dbSNP:61733416
1416 1416 a, g dbSNP:761696930
1423 1423 c, t dbSNP:751038951
1425 1425 c, t dbSNP:763718170
1426 1426 c, t dbSNP:762635201
1451 1451 c, g, t dbSNP:769635292
1465 1465 a, g dbSNP:759928495
1467 1467 c, t dbSNP:776905089
1477 1477 c, t dbSNP:373065228
1482 1482 c, t dbSNP:61733415
1494 1494 a, g dbSNP:747504890
1497 1497 c, t dbSNP:777784952
1505 1505 a, c dbSNP:772156175
1508 1508 c, g dbSNP:748305332
1532 1532 c, t dbSNP:779104297
1534 1534 a, g dbSNP:755252289
1559 1559 c, t dbSNP:750210029
1560 1560 c, t dbSNP:780880487
1562 1562 c, t dbSNP:574271017
1571 1571 c, t dbSNP:137945099
1609 1609 c, t dbSNP:201085318
1614 1614 c, t dbSNP:751464195
1631 1631 c, t dbSNP:368944844
1634 1634 c, t dbSNP:762545906
1653 1653 c, g dbSNP:752310933
1655 1655 c, g dbSNP:764957396
1664 1664 c, t dbSNP:563883873
1688 1688 c, t dbSNP:376214839
1704 1704 c, t dbSNP:377619754
1742 1742 g, t dbSNP:4941195
1777 1777 c, t dbSNP:189218577
1778 1778 a, g dbSNP:553199829
1804 1804 c, t dbSNP:185187774
1814 1814 c, t dbSNP:767397631
1821 1821 a, t dbSNP:573888693
1839 1839 c, t dbSNP:557057039
1860 1860 a, c dbSNP:537168875
1914 1914 a, g dbSNP:571132328
1918 1918 -, a dbSNP:544384134
1973 1973 a, g dbSNP:759666859
2010 2010 c, t dbSNP:565663208
2061 2061 a, g dbSNP:751641309
2074 2074 a, g dbSNP:180785978
2130 2130 a, g dbSNP:766600787
2142 2142 a, g dbSNP:149016023

Target ORF information:

RefSeq Version XM_011526135
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens B-cell CLL/lymphoma 2 (BCL2), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011526135

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCGCACG CTGGGAGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAT 
TATAAGCTGT CGCAGAGGGG CTACGAGTGG GATGCGGGAG ATGTGGGCGC CGCGCCCCCG
GGGGCCGCCC CCGCACCGGG CATCTTCTCC TCCCAGCCCG GGCACACGCC CCATCCAGCC
GCATCCCGGG ACCCGGTCGC CAGGACCTCG CCGCTGCAGA CCCCGGCTGC CCCCGGCGCC
GCCGCGGGGC CTGCGCTCAG CCCGGTGCCA CCTGTGGTCC ACCTGACCCT CCGCCAGGCC
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC
CTGACGCCCT TCACCGCGCG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAC
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC
CTGAACCGGC ACCTGCACAC CTGGATCCAG GATAACGGAG GCTGGGATGC CCTTGTGTTA
TTTGCAGGGC TAAGGGAGTT TCAGCATGGG CCAGGAAACT ACTTTCTTAA AAGAAACTTA
AACGCGAAAT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25505
Accession Version NM_000633.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 720bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu25505D_COA.pdf (pdf)
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product apoptosis regulator Bcl-2 alpha isoform
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC021803.8, BC027258.1 and AC022726.13. This sequence is a reference standard in the RefSeqGene project. On Aug 10, 2005 this sequence version replaced gi:4557354. Summary: This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (alpha) represents the longer transcript and encodes the longer isoform (alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta regulatory uORF :: PMID: 9645350 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC027258.1, X06487.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962337, SAMEA962343 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCGCACG CTGGGAGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAT 
TATAAGCTGT CGCAGAGGGG CTACGAGTGG GATGCGGGAG ATGTGGGCGC CGCGCCCCCG
GGGGCCGCCC CCGCACCGGG CATCTTCTCC TCCCAGCCCG GGCACACGCC CCATCCAGCC
GCATCCCGGG ACCCGGTCGC CAGGACCTCG CCGCTGCAGA CCCCGGCTGC CCCCGGCGCC
GCCGCGGGGC CTGCGCTCAG CCCGGTGCCA CCTGTGGTCC ACCTGACCCT CCGCCAGGCC
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC
CTGACGCCCT TCACCGCGCG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAC
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC
CTGAACCGGC ACCTGCACAC CTGGATCCAG GATAACGGAG GCTGGGATGC CTTTGTGGAA
CTGTACGGCC CCAGCATGCG GCCTCTGTTT GATTTCTCCT GGCTGTCTCT GAAGACTCTG
CTCAGTTTGG CCCTGGTGGG AGCTTGCATC ACCCTGGGTG CCTATCTGGG CCACAAGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000624.2
CDS494..1213
Misc Feature(1)375..410(+)
Misc Feature(2)392..394(+)
Misc Feature(3)494..1210(+)
Misc Feature(4)512..592(+)
Misc Feature(5)521..583(+)
Misc Feature(6)563..565(+)
Misc Feature(7)593..598(+)
Misc Feature(8)593..595(+)
Misc Feature(9)659..661(+)
Misc Feature(10)698..700(+)
Misc Feature(11)698..700(+)
Misc Feature(12)701..703(+)
Misc Feature(13)701..703(+)
Misc Feature(14)701..703(+)
Misc Feature(15)713..715(+)
Misc Feature(16)749..1096(+)
Misc Feature(17)752..754(+)
Misc Feature(18)752..754(+)
Misc Feature(19)752..754(+)
Misc Feature(20)770..814(+)
Misc Feature(21)782..808(+)
Misc Feature(22)791..1096(+)
Misc Feature(23)899..958(+)
Misc Feature(24)899..955(+)
Misc Feature(25)1052..1099(+)
Misc Feature(26)1052..1096(+)
Misc Feature(27)1127..1192(+)
Exon (1)1..207
Gene:BCL2
Gene Synonym:
Exon (2)208..1078
Gene:BCL2
Gene Synonym:
Exon (3)1079..6492
Gene:BCL2
Gene Synonym:
Translation
Position Chain Variation Link
13 13 a, g dbSNP:566462049
18 18 -, a dbSNP:34360708
19 19 c, t dbSNP:779249610
65 65 c, g dbSNP:1473418
71 71 c, g dbSNP:192114822
78 78 c, g dbSNP:560936021
123 123 g, t dbSNP:34223591
126 126 g, t dbSNP:550615446
176 176 a, g dbSNP:530542966
202 202 -, a dbSNP:757809447
350 350 a, g dbSNP:772250802
351 351 c, t dbSNP:765740867
361 361 c, t dbSNP:55805819
425 425 c, t dbSNP:552075974
445 445 a, g dbSNP:780722376
452 452 c, t dbSNP:114373512
459 459 c, t dbSNP:764979303
466 466 c, g dbSNP:751238437
468 468 c, t dbSNP:778057839
477 477 -, c dbSNP:769934430
480 480 g, t dbSNP:758699052
483 483 c, g dbSNP:753168582
485 485 c, t dbSNP:765644873
486 486 c, t dbSNP:760135041
493 493 a, g dbSNP:193014588
499 499 g, t dbSNP:766594821
501 501 a, g dbSNP:557714365
502 502 c, t dbSNP:773580172
503 503 g, t dbSNP:768498963
514 514 a, g dbSNP:1801018
516 516 a, g dbSNP:775404824
525 525 a, g dbSNP:769588208
536 536 c, g dbSNP:745851862
541 541 a, g dbSNP:776360417
542 542 a, g dbSNP:780634396
544 544 a, g dbSNP:565741123
548 548 a, c dbSNP:746600883
551 551 a, c dbSNP:777401949
553 553 g, t dbSNP:758123306
565 565 g, t dbSNP:752978070
572 572 a, g dbSNP:779372254
577 577 c, t dbSNP:755397980
583 583 a, g dbSNP:754418219
584 584 c, g dbSNP:766508625
587 587 c, g dbSNP:760840889
588 588 c, g dbSNP:546449806
590 590 a, g dbSNP:767612613
592 592 -, a dbSNP:759748900
593 593 c, g, t dbSNP:540701354
595 595 c, t dbSNP:762137430
596 596 a, g dbSNP:775108608
604 604 a, c dbSNP:769656259
610 610 a, c, t dbSNP:776558326
612 612 c, t dbSNP:770925898
613 613 a, g dbSNP:746504624
614 614 a, g dbSNP:777309369
620 620 a, g dbSNP:1800477
625 625 a, c dbSNP:747863570
626 626 g, t dbSNP:779096658
627 627 c, g dbSNP:755382644
629 629 c, g dbSNP:749745319
630 630 c, t dbSNP:369277721
631 631 a, g dbSNP:756734795
632 632 a, g dbSNP:368344129
637 637 c, t dbSNP:767668991
640 640 c, t dbSNP:200972039
646 646 c, t dbSNP:751822316
652 652 c, t dbSNP:764877973
656 656 c, t dbSNP:759325300
658 658 c, t dbSNP:776473075
660 660 c, g dbSNP:766277782
674 674 a, g, t dbSNP:772554403
678 678 a, c, t dbSNP:747696230
680 680 c, t dbSNP:774056251
686 686 c, t dbSNP:768259110
689 689 a, g dbSNP:749649089
692 692 a, g dbSNP:780606244
693 693 c, t dbSNP:756650851
695 695 a, g dbSNP:746401408
697 697 a, g dbSNP:781072794
703 703 a, g dbSNP:770596713
706 706 a, g dbSNP:376115863
717 717 c, t dbSNP:757360142
718 718 a, g, t dbSNP:764359199
724 724 c, t dbSNP:758673021
725 725 c, t dbSNP:753603356
728 728 c, g dbSNP:766196038
732 732 c, t dbSNP:760559670
738 738 c, t dbSNP:201827119
741 741 a, g dbSNP:375594294
742 742 a, g dbSNP:766872577
743 743 c, t dbSNP:761382113
745 745 g, t dbSNP:773791165
751 751 c, t dbSNP:768392602
752 752 a, g dbSNP:748978916
754 754 c, g, t dbSNP:770293857
756 756 c, g, t dbSNP:781718615
766 766 c, t dbSNP:771396277
771 771 c, t dbSNP:565014924
772 772 c, g dbSNP:747085111
774 774 a, c dbSNP:551395951
776 776 c, g, t dbSNP:373889054
779 779 a, g dbSNP:752942153
781 781 c, g dbSNP:557922819
783 783 c, t dbSNP:528042823
786 786 g, t dbSNP:755908754
789 789 a, g dbSNP:559397524
792 792 c, t dbSNP:750253286
793 793 c, t dbSNP:61733416
797 797 a, g dbSNP:761696930
804 804 c, t dbSNP:751038951
806 806 c, t dbSNP:763718170
807 807 c, t dbSNP:762635201
832 832 c, g, t dbSNP:769635292
846 846 a, g dbSNP:759928495
848 848 c, t dbSNP:776905089
858 858 c, t dbSNP:373065228
863 863 c, t dbSNP:61733415
875 875 a, g dbSNP:747504890
878 878 c, t dbSNP:777784952
886 886 a, c dbSNP:772156175
889 889 c, g dbSNP:748305332
913 913 c, t dbSNP:779104297
915 915 a, g dbSNP:755252289
940 940 c, t dbSNP:750210029
941 941 c, t dbSNP:780880487
943 943 c, t dbSNP:574271017
952 952 c, t dbSNP:137945099
990 990 c, t dbSNP:201085318
995 995 c, t dbSNP:751464195
1012 1012 c, t dbSNP:368944844
1015 1015 c, t dbSNP:762545906
1034 1034 c, g dbSNP:752310933
1036 1036 c, g dbSNP:764957396
1045 1045 c, t dbSNP:563883873
1069 1069 c, t dbSNP:376214839
1084 1084 c, t dbSNP:770109390
1094 1094 c, t dbSNP:143441810
1100 1100 a, g dbSNP:148811059
1105 1105 c, t dbSNP:777747659
1109 1109 a, g dbSNP:758367430
1112 1112 c, t dbSNP:748122615
1113 1113 a, g dbSNP:369294037
1114 1114 a, g dbSNP:754454655
1124 1124 c, g dbSNP:753457022
1154 1154 c, t dbSNP:574148249
1157 1157 a, g dbSNP:113163226
1165 1165 c, t dbSNP:568070522
1168 1168 c, g dbSNP:755841158
1181 1181 a, g dbSNP:750755544
1187 1187 c, t dbSNP:767891249
1191 1191 a, g dbSNP:376149674
1195 1195 c, t dbSNP:537410586
1202 1202 a, g, t dbSNP:138465313
1207 1207 c, t dbSNP:747762568
1208 1208 a, g dbSNP:762925672
1209 1209 a, g dbSNP:775686567
1215 1215 a, g dbSNP:373385925
1218 1218 a, g dbSNP:769872480
1226 1226 g, t dbSNP:759865650
1230 1230 c, g dbSNP:375217502
1235 1235 a, c dbSNP:200716283
1257 1257 a, c, g dbSNP:371826692
1259 1259 a, g dbSNP:778714573
1261 1261 a, c dbSNP:768697984
1352 1352 a, g dbSNP:537472384
1363 1363 a, c dbSNP:190262124
1373 1373 -, ca dbSNP:140093696
1399 1399 c, t dbSNP:558855674
1400 1400 a, g dbSNP:538519659
1403 1403 a, g dbSNP:560105440
1427 1427 -, g dbSNP:747788648
1452 1452 -, tta dbSNP:568132412
1467 1467 a, t dbSNP:779804119
1471 1471 a, t dbSNP:4987842
1480 1480 a, g dbSNP:754709154
1485 1485 a, c dbSNP:546351646
1513 1513 c, t dbSNP:543205988
1516 1516 c, t dbSNP:745804189
1518 1518 a, t dbSNP:567047705
1530 1530 a, g dbSNP:4987843
1536 1536 c, t dbSNP:757162378
1537 1537 a, t dbSNP:185016407
1539 1539 c, t dbSNP:571116372
1565 1565 c, g dbSNP:552053320
1573 1573 a, g dbSNP:753911659
1575 1575 a, g dbSNP:764118446
1578 1578 g, t dbSNP:116616724
1602 1602 a, g dbSNP:559912455
1624 1624 c, t dbSNP:367947421
1647 1647 c, t dbSNP:753026404
1703 1703 g, t dbSNP:543279713
1734 1734 a, c dbSNP:113379949
1739 1739 a, g dbSNP:563822390
1753 1753 g, t dbSNP:41301945
1758 1758 -, g, gg dbSNP:774070039
1765 1765 a, c dbSNP:577979796
1779 1779 c, t dbSNP:377403870
1793 1793 c, t dbSNP:759914693
1798 1798 a, c dbSNP:4987844
1841 1841 a, g dbSNP:191858049
1879 1879 c, t dbSNP:573086110
1880 1880 a, g dbSNP:3744937
1882 1882 a, c dbSNP:536087403
1883 1883 a, g dbSNP:4987845
1890 1890 a, g dbSNP:3744936
1893 1893 a, g dbSNP:4987846
1894 1894 a, t dbSNP:768103380
1917 1917 c, t dbSNP:746655788
1919 1919 c, g dbSNP:775194968
1943 1943 a, g dbSNP:150966214
1997 1997 a, g dbSNP:1016860
2011 2011 c, t dbSNP:745609712
2020 2020 a, g dbSNP:538259407
2031 2031 a, g dbSNP:528074102
2050 2050 a, g dbSNP:4987847
2108 2108 a, g dbSNP:141174596
2117 2117 c, t dbSNP:777893248
2118 2118 a, g, t dbSNP:116117134
2129 2129 a, g dbSNP:563834758
2140 2140 c, t dbSNP:543902439
2154 2154 c, t dbSNP:756328207
2155 2155 a, g dbSNP:4987848
2161 2161 a, g dbSNP:781507624
2188 2188 c, t dbSNP:755273824
2189 2189 a, g dbSNP:564586762
2198 2198 a, g dbSNP:541093229
2238 2238 c, t dbSNP:751914066
2239 2239 a, g dbSNP:572399575
2240 2240 a, g dbSNP:552888307
2257 2257 c, t dbSNP:371590615
2278 2278 c, t dbSNP:2078303
2302 2302 a, g dbSNP:144527240
2317 2317 a, g dbSNP:573504228
2322 2322 c, g dbSNP:556708975
2333 2333 a, g dbSNP:537166590
2385 2385 a, c dbSNP:766695698
2391 2391 a, g dbSNP:3744935
2399 2399 g, t dbSNP:4987849
2416 2416 c, t dbSNP:750951587
2417 2417 a, g dbSNP:1564483
2462 2462 a, c dbSNP:565671491
2469 2469 a, c dbSNP:549709463
2505 2505 c, t dbSNP:145009492
2506 2506 a, g dbSNP:369042063
2510 2510 a, g dbSNP:760985165
2518 2518 a, t dbSNP:552258175
2526 2526 c, t dbSNP:187455180
2548 2548 a, g dbSNP:771669959
2555 2555 a, g dbSNP:4987850
2568 2568 a, c dbSNP:149940394
2592 2592 a, g dbSNP:139755068
2658 2658 a, g dbSNP:1050618
2670 2670 a, c dbSNP:371452924
2678 2678 a, g dbSNP:150653783
2725 2725 c, t dbSNP:774172635
2739 2739 a, c dbSNP:770687321
2747 2747 -, a dbSNP:202112192
2775 2775 c, t dbSNP:561700123
2784 2784 c, t dbSNP:181495000
2794 2794 a, t dbSNP:180847268
2795 2795 a, g, t dbSNP:113207678
2803 2803 -, a dbSNP:5825508
2812 2812 a, g dbSNP:4987851
2877 2877 -, t dbSNP:530340306
2890 2890 a, t dbSNP:543280509
2906 2906 c, t dbSNP:777503643
2938 2938 a, g dbSNP:550786606
2976 2976 c, g dbSNP:577765262
3041 3041 -, t dbSNP:369915593
3041 3041 a, g dbSNP:769867822
3048 3048 -, t dbSNP:777364862
3059 3059 c, g dbSNP:557610580
3103 3103 a, g dbSNP:534676168
3111 3111 c, t dbSNP:374062303
3123 3123 a, g dbSNP:781454557
3142 3142 a, g dbSNP:184698169
3150 3150 a, g dbSNP:4987852
3249 3249 c, t dbSNP:181211289
3289 3289 c, t dbSNP:570388509
3311 3311 a, g dbSNP:550443719
3319 3319 c, t dbSNP:142756703
3320 3320 a, g dbSNP:139528731
3341 3341 g, t dbSNP:547576951
3375 3375 a, g dbSNP:188797102
3381 3381 c, t dbSNP:780455853
3386 3386 g, t dbSNP:760051790
3406 3406 c, t dbSNP:373487515
3416 3416 a, g dbSNP:4987853
3439 3439 a, g dbSNP:548212819
3445 3445 a, g dbSNP:4987854
3446 3446 g, t dbSNP:765680013
3482 3482 c, g dbSNP:762334439
3522 3522 a, g dbSNP:4987855
3527 3527 a, c dbSNP:767060901
3550 3550 a, g dbSNP:117640948
3575 3575 -, a dbSNP:776245002
3577 3577 a, g dbSNP:4987856
3624 3624 c, t dbSNP:146379399
3625 3625 a, g dbSNP:529635154
3627 3627 c, t dbSNP:4987857
3654 3654 a, c dbSNP:142265965
3655 3655 c, g dbSNP:773083543
3686 3686 c, t dbSNP:769814545
3715 3715 a, g dbSNP:748138761
3741 3741 a, g dbSNP:148341476
3840 3840 a, g dbSNP:17070680
3874 3874 c, t dbSNP:563706706
3880 3880 c, t dbSNP:768917760
3923 3923 a, g dbSNP:747252845
3933 3933 a, c dbSNP:780076934
3935 3935 c, t dbSNP:544015519
4029 4029 c, t dbSNP:746174746
4033 4033 a, c dbSNP:375734256
4054 4054 -, t dbSNP:374357488
4097 4097 c, t dbSNP:183691931
4147 4147 c, t dbSNP:570978368
4153 4153 -, ctt dbSNP:555342230
4246 4246 -, t dbSNP:780393617
4263 4263 a, t dbSNP:757735599
4277 4277 c, t dbSNP:4987858
4321 4321 c, t dbSNP:534110924
4338 4338 c, t dbSNP:778690174
4393 4393 c, t dbSNP:137980414
4396 4396 c, t dbSNP:41306972
4411 4411 c, t dbSNP:377487726
4414 4414 a, g dbSNP:4987859
4451 4451 a, g dbSNP:569192313
4463 4463 c, t dbSNP:549811647
4469 4469 c, t dbSNP:533229451
4484 4484 a, g dbSNP:4987860
4499 4499 a, g dbSNP:544557307
4507 4507 c, t dbSNP:4987861
4519 4519 c, t dbSNP:188618198
4525 4525 c, t dbSNP:762697811
4572 4572 c, t dbSNP:773113174
4577 4577 c, t dbSNP:552589178
4594 4594 a, c dbSNP:146967889
4644 4644 a, t dbSNP:541905026
4678 4678 c, g dbSNP:575950034
4686 4686 c, t dbSNP:556095664
4698 4698 a, g dbSNP:546195385
4724 4724 c, t dbSNP:141080340
4725 4725 a, g, t dbSNP:147777348
4739 4739 a, t dbSNP:756346453
4764 4764 a, g dbSNP:4987862
4771 4771 c, t dbSNP:185704013
4790 4790 c, t dbSNP:193151005
4815 4815 a, g dbSNP:554654182
4834 4834 c, t dbSNP:537903627
4850 4850 c, t dbSNP:200305018
4876 4876 a, t dbSNP:569181589
4885 4885 -, aaactc dbSNP:370395757
4885 4885 a, g dbSNP:755532366
4896 4896 -, aatt dbSNP:572382935
4897 4897 a, t dbSNP:146086350
4915 4915 a, g dbSNP:748870593
4923 4923 g, t dbSNP:532390266
4926 4926 -, g dbSNP:142611734
4932 4932 -, agac dbSNP:374368978
4932 4932 a, t dbSNP:570769069
4988 4988 a, g dbSNP:187334313
4994 4994 g, t dbSNP:4987863
5032 5032 a, c dbSNP:561816906
5080 5080 c, g dbSNP:768646925
5082 5082 c, t dbSNP:541966669
5114 5114 a, c dbSNP:767916494
5118 5118 c, t dbSNP:183000521
5163 5163 c, t dbSNP:140875276
5167 5167 a, g dbSNP:539609896
5175 5175 c, t dbSNP:45520140
5183 5183 a, g dbSNP:77530335
5184 5184 -, t dbSNP:372843127
5184 5184 a, t dbSNP:76721111
5190 5190 -, t dbSNP:386387940
5194 5194 -, t, tt dbSNP:34811186
5194 5194 a, t dbSNP:113158648
5195 5195 a, t dbSNP:77938688
5197 5197 -, a dbSNP:554443203
5294 5294 g, t dbSNP:554085830
5299 5299 c, g dbSNP:747006093
5305 5305 a, c dbSNP:375819800
5312 5312 c, t dbSNP:775666508
5337 5337 g, t dbSNP:4987864
5438 5438 a, g dbSNP:371518171
5503 5503 a, g dbSNP:554717379
5513 5513 c, t dbSNP:370035615
5514 5514 a, g dbSNP:4987865
5567 5567 a, t dbSNP:190011740
5601 5601 -, a dbSNP:45489598
5655 5655 a, g dbSNP:539061071
5690 5690 c, g dbSNP:566710209
5697 5697 -, t dbSNP:376082112
5713 5713 c, t dbSNP:547715314
5721 5721 a, g dbSNP:571076818
5729 5729 -, a dbSNP:765856143
5745 5745 a, t dbSNP:45468492
5750 5750 a, g dbSNP:4987866
5766 5766 c, g dbSNP:573907712
5814 5814 c, t dbSNP:749655144
5837 5837 a, c dbSNP:577768081
5856 5856 c, t dbSNP:187081713
5876 5876 a, g dbSNP:778194812
5883 5883 c, g dbSNP:756666269
5898 5898 a, c dbSNP:556851434
5905 5905 a, g dbSNP:142616439
5944 5944 -, c dbSNP:762163340
5958 5958 a, c dbSNP:562649519
5977 5977 c, t dbSNP:551977130
5999 5999 a, g dbSNP:4987867
6021 6021 c, t dbSNP:56041624
6068 6068 -, g dbSNP:760256968
6082 6082 -, t dbSNP:34143126
6121 6121 g, t dbSNP:765955215
6127 6127 a, g dbSNP:540538416
6140 6140 a, g dbSNP:148888145
6166 6166 a, g dbSNP:757967145
6199 6199 a, g dbSNP:750160246
6220 6220 g, t dbSNP:561221675
6223 6223 a, t dbSNP:544821608
6254 6254 c, t dbSNP:765122650
6262 6262 a, c dbSNP:4987868
6269 6269 a, g dbSNP:776584611
6273 6273 c, t dbSNP:559015384
6285 6285 c, g dbSNP:182433677
6326 6326 c, t dbSNP:45503395
6341 6341 a, c dbSNP:4987869
6378 6378 a, g dbSNP:770721911
6383 6383 -, t dbSNP:79620975
6384 6384 -, t dbSNP:4987870
6394 6394 c, t dbSNP:535174883
6408 6408 c, t dbSNP:536627558
6420 6420 a, g dbSNP:775407079
6444 6444 c, t dbSNP:566072775

Target ORF information:

RefSeq Version NM_000633
Organism Homo sapiens (human)
Definition Homo sapiens B-cell CLL/lymphoma 2 (BCL2), transcript variant alpha, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000633

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCGCACG CTGGGAGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAT 
TATAAGCTGT CGCAGAGGGG CTACGAGTGG GATGCGGGAG ATGTGGGCGC CGCGCCCCCG
GGGGCCGCCC CCGCACCGGG CATCTTCTCC TCCCAGCCCG GGCACACGCC CCATCCAGCC
GCATCCCGGG ACCCGGTCGC CAGGACCTCG CCGCTGCAGA CCCCGGCTGC CCCCGGCGCC
GCCGCGGGGC CTGCGCTCAG CCCGGTGCCA CCTGTGGTCC ACCTGACCCT CCGCCAGGCC
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC
CTGACGCCCT TCACCGCGCG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAC
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC
CTGAACCGGC ACCTGCACAC CTGGATCCAG GATAACGGAG GCTGGGATGC CTTTGTGGAA
CTGTACGGCC CCAGCATGCG GCCTCTGTTT GATTTCTCCT GGCTGTCTCT GAAGACTCTG
CTCAGTTTGG CCCTGGTGGG AGCTTGCATC ACCCTGGGTG CCTATCTGGG CCACAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25321
Accession Version NM_000657.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 618bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product apoptosis regulator Bcl-2 beta isoform
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC021803.8 and AI401297.1. On Aug 10, 2005 this sequence version replaced gi:4557356. Summary: This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (beta) differs in the 3' UTR and coding region compared to variant alpha. The resulting isoform (beta) is shorter and has a distinct C-terminus compared to isoform alpha. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta regulatory uORF :: PMID: 9645350 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BQ056709.1, BG258362.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962337, SAMEA962343 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGGCGCACG CTGGGAGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAT 
TATAAGCTGT CGCAGAGGGG CTACGAGTGG GATGCGGGAG ATGTGGGCGC CGCGCCCCCG
GGGGCCGCCC CCGCACCGGG CATCTTCTCC TCCCAGCCCG GGCACACGCC CCATCCAGCC
GCATCCCGGG ACCCGGTCGC CAGGACCTCG CCGCTGCAGA CCCCGGCTGC CCCCGGCGCC
GCCGCGGGGC CTGCGCTCAG CCCGGTGCCA CCTGTGGTCC ACCTGACCCT CCGCCAGGCC
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC
CTGACGCCCT TCACCGCGCG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAC
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC
CTGAACCGGC ACCTGCACAC CTGGATCCAG GATAACGGAG GCTGGGTAGG TGCACTTGGT
GATGTGAGTC TGGGCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000648.2
CDS494..1111
Misc Feature(1)375..410(+)
Misc Feature(2)392..394(+)
Misc Feature(3)494..>1078(+)
Misc Feature(4)512..592(+)
Misc Feature(5)521..583(+)
Misc Feature(6)563..565(+)
Misc Feature(7)593..598(+)
Misc Feature(8)593..595(+)
Misc Feature(9)659..661(+)
Misc Feature(10)698..700(+)
Misc Feature(11)698..700(+)
Misc Feature(12)701..703(+)
Misc Feature(13)701..703(+)
Misc Feature(14)713..715(+)
Misc Feature(15)749..1084(+)
Misc Feature(16)752..754(+)
Misc Feature(17)752..754(+)
Misc Feature(18)752..754(+)
Misc Feature(19)752..754(+)
Misc Feature(20)770..814(+)
Misc Feature(21)782..808(+)
Misc Feature(22)791..964(+)
Misc Feature(23)899..958(+)
Misc Feature(24)899..955(+)
Misc Feature(25)1052..1084(+)
Exon (1)1..207
Gene:BCL2
Gene Synonym:
Exon (2)208..1206
Gene:BCL2
Gene Synonym:
Translation
Position Chain Variation Link
13 13 a, g dbSNP:566462049
18 18 -, a dbSNP:34360708
19 19 c, t dbSNP:779249610
65 65 c, g dbSNP:1473418
71 71 c, g dbSNP:192114822
78 78 c, g dbSNP:560936021
123 123 g, t dbSNP:34223591
126 126 g, t dbSNP:550615446
176 176 a, g dbSNP:530542966
202 202 -, a dbSNP:757809447
350 350 a, g dbSNP:772250802
351 351 c, t dbSNP:765740867
361 361 c, t dbSNP:55805819
425 425 c, t dbSNP:552075974
445 445 a, g dbSNP:780722376
452 452 c, t dbSNP:114373512
459 459 c, t dbSNP:764979303
466 466 c, g dbSNP:751238437
468 468 c, t dbSNP:778057839
477 477 -, c dbSNP:769934430
480 480 g, t dbSNP:758699052
483 483 c, g dbSNP:753168582
485 485 c, t dbSNP:765644873
486 486 c, t dbSNP:760135041
493 493 a, g dbSNP:193014588
499 499 g, t dbSNP:766594821
501 501 a, g dbSNP:557714365
502 502 c, t dbSNP:773580172
503 503 g, t dbSNP:768498963
514 514 a, g dbSNP:1801018
516 516 a, g dbSNP:775404824
525 525 a, g dbSNP:769588208
536 536 c, g dbSNP:745851862
541 541 a, g dbSNP:776360417
542 542 a, g dbSNP:780634396
544 544 a, g dbSNP:565741123
548 548 a, c dbSNP:746600883
551 551 a, c dbSNP:777401949
553 553 g, t dbSNP:758123306
565 565 g, t dbSNP:752978070
572 572 a, g dbSNP:779372254
577 577 c, t dbSNP:755397980
583 583 a, g dbSNP:754418219
584 584 c, g dbSNP:766508625
587 587 c, g dbSNP:760840889
588 588 c, g dbSNP:546449806
590 590 a, g dbSNP:767612613
592 592 -, a dbSNP:759748900
593 593 c, g, t dbSNP:540701354
595 595 c, t dbSNP:762137430
596 596 a, g dbSNP:775108608
604 604 a, c dbSNP:769656259
610 610 a, c, t dbSNP:776558326
612 612 c, t dbSNP:770925898
613 613 a, g dbSNP:746504624
614 614 a, g dbSNP:777309369
620 620 a, g dbSNP:1800477
625 625 a, c dbSNP:747863570
626 626 g, t dbSNP:779096658
627 627 c, g dbSNP:755382644
629 629 c, g dbSNP:749745319
630 630 c, t dbSNP:369277721
631 631 a, g dbSNP:756734795
632 632 a, g dbSNP:368344129
637 637 c, t dbSNP:767668991
640 640 c, t dbSNP:200972039
646 646 c, t dbSNP:751822316
652 652 c, t dbSNP:764877973
656 656 c, t dbSNP:759325300
658 658 c, t dbSNP:776473075
660 660 c, g dbSNP:766277782
674 674 a, g, t dbSNP:772554403
678 678 a, c, t dbSNP:747696230
680 680 c, t dbSNP:774056251
686 686 c, t dbSNP:768259110
689 689 a, g dbSNP:749649089
692 692 a, g dbSNP:780606244
693 693 c, t dbSNP:756650851
695 695 a, g dbSNP:746401408
697 697 a, g dbSNP:781072794
703 703 a, g dbSNP:770596713
706 706 a, g dbSNP:376115863
717 717 c, t dbSNP:757360142
718 718 a, g, t dbSNP:764359199
724 724 c, t dbSNP:758673021
725 725 c, t dbSNP:753603356
728 728 c, g dbSNP:766196038
732 732 c, t dbSNP:760559670
738 738 c, t dbSNP:201827119
741 741 a, g dbSNP:375594294
742 742 a, g dbSNP:766872577
743 743 c, t dbSNP:761382113
745 745 g, t dbSNP:773791165
751 751 c, t dbSNP:768392602
752 752 a, g dbSNP:748978916
754 754 c, g, t dbSNP:770293857
756 756 c, g, t dbSNP:781718615
766 766 c, t dbSNP:771396277
771 771 c, t dbSNP:565014924
772 772 c, g dbSNP:747085111
774 774 a, c dbSNP:551395951
776 776 c, g, t dbSNP:373889054
779 779 a, g dbSNP:752942153
781 781 c, g dbSNP:557922819
783 783 c, t dbSNP:528042823
786 786 g, t dbSNP:755908754
789 789 a, g dbSNP:559397524
792 792 c, t dbSNP:750253286
793 793 c, t dbSNP:61733416
797 797 a, g dbSNP:761696930
804 804 c, t dbSNP:751038951
806 806 c, t dbSNP:763718170
807 807 c, t dbSNP:762635201
832 832 c, g, t dbSNP:769635292
846 846 a, g dbSNP:759928495
848 848 c, t dbSNP:776905089
858 858 c, t dbSNP:373065228
863 863 c, t dbSNP:61733415
875 875 a, g dbSNP:747504890
878 878 c, t dbSNP:777784952
886 886 a, c dbSNP:772156175
889 889 c, g dbSNP:748305332
913 913 c, t dbSNP:779104297
915 915 a, g dbSNP:755252289
940 940 c, t dbSNP:750210029
941 941 c, t dbSNP:780880487
943 943 c, t dbSNP:574271017
952 952 c, t dbSNP:137945099
990 990 c, t dbSNP:201085318
995 995 c, t dbSNP:751464195
1012 1012 c, t dbSNP:368944844
1015 1015 c, t dbSNP:762545906
1034 1034 c, g dbSNP:752310933
1036 1036 c, g dbSNP:764957396
1045 1045 c, t dbSNP:563883873
1069 1069 c, t dbSNP:376214839
1091 1091 g, t dbSNP:771226616
1094 1094 a, g dbSNP:371685903
1097 1097 g, t dbSNP:557000269
1101 1101 a, g dbSNP:377753316
1108 1108 c, g dbSNP:772635691
1109 1109 c, t dbSNP:748213542
1115 1115 c, t dbSNP:543688353
1117 1117 c, t dbSNP:768883638
1119 1119 a, g, t dbSNP:780981306
1124 1124 a, g dbSNP:760535243
1126 1126 a, g dbSNP:757005204
1131 1131 a, g dbSNP:746865248
1133 1133 a, g dbSNP:777837472
1134 1134 a, g dbSNP:758455722
1135 1135 g, t dbSNP:752219121
1139 1139 a, c, g dbSNP:4987710
1150 1150 a, g dbSNP:376849362
1153 1153 a, t dbSNP:557876106
1157 1157 g, t dbSNP:760944654
1166 1166 a, g dbSNP:541138483
1183 1183 a, c, t dbSNP:572076117
1198 1198 c, g dbSNP:555499082
1202 1202 c, t dbSNP:772028186

Target ORF information:

RefSeq Version NM_000657
Organism Homo sapiens (human)
Definition Homo sapiens B-cell CLL/lymphoma 2 (BCL2), transcript variant beta, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000657

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCGCACG CTGGGAGAAC AGGGTACGAT AACCGGGAGA TAGTGATGAA GTACATCCAT 
TATAAGCTGT CGCAGAGGGG CTACGAGTGG GATGCGGGAG ATGTGGGCGC CGCGCCCCCG
GGGGCCGCCC CCGCACCGGG CATCTTCTCC TCCCAGCCCG GGCACACGCC CCATCCAGCC
GCATCCCGGG ACCCGGTCGC CAGGACCTCG CCGCTGCAGA CCCCGGCTGC CCCCGGCGCC
GCCGCGGGGC CTGCGCTCAG CCCGGTGCCA CCTGTGGTCC ACCTGACCCT CCGCCAGGCC
GGCGACGACT TCTCCCGCCG CTACCGCCGC GACTTCGCCG AGATGTCCAG CCAGCTGCAC
CTGACGCCCT TCACCGCGCG GGGACGCTTT GCCACGGTGG TGGAGGAGCT CTTCAGGGAC
GGGGTGAACT GGGGGAGGAT TGTGGCCTTC TTTGAGTTCG GTGGGGTCAT GTGTGTGGAG
AGCGTCAACC GGGAGATGTC GCCCCTGGTG GACAACATCG CCCTGTGGAT GACTGAGTAC
CTGAACCGGC ACCTGCACAC CTGGATCCAG GATAACGGAG GCTGGGTAGG TGCACTTGGT
GATGTGAGTC TGGGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.