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RPS17 cDNA ORF clone, Homo sapiens (human)

Gene Symbol RPS17
Entrez Gene ID 6218
Full Name ribosomal protein S17
Synonyms DBA4, RPS17L, RPS17L1, RPS17L2, S17
General protein information
Preferred Names
40S ribosomal protein S17
Names
40S ribosomal protein S17
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

15

15q

Summary Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S17E family of ribosomal proteins and is located in the cytoplasm. Mutations in this gene cause Diamond-Blackfan anemia 4. Alternative splicing of this gene results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Apr 2014]. lac of sum
Disorder MIM:

180472

Disorder Html: Diamond-Blackfan anemia 4, 612527 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001021 NP_001012 40S ribosomal protein S17

KEGG
hsa03010 Ribosome
hsa_M00177 Ribosome, eukaryotes
Reactome
R-HSA-1643685 Disease
R-HSA-168254 Influenza Infection
R-HSA-168255 Influenza Life Cycle
R-HSA-5663205 Infectious disease
R-HSA-168273 Influenza Viral RNA Transcription and Replication
R-HSA-192823 Viral mRNA Translation
R-HSA-392499 Metabolism of proteins
R-HSA-74160 Gene Expression
R-HSA-72737 Cap-dependent Translation Initiation
R-HSA-72662 Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S
R-HSA-72613 Eukaryotic Translation Initiation
R-HSA-72649 Translation initiation complex formation
R-HSA-156902 Peptide chain elongation
R-HSA-156842 Eukaryotic Translation Elongation
R-HSA-72764 Eukaryotic Translation Termination
R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-HSA-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-HSA-72766 Translation
R-HSA-1799339 SRP-dependent cotranslational protein targeting to membrane
R-HSA-927802 Nonsense-Mediated Decay (NMD)
R-HSA-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)
R-HSA-72689 Formation of a pool of free 40S subunits
R-HSA-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-HSA-72702 Ribosomal scanning and start codon recognition
R-HSA-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
WikiPathways
WP477 Cytoplasmic Ribosomal Proteins


Homo sapiens (human) RPS17 NP_001012.1
Pan troglodytes (chimpanzee) RPS17 XP_525570.2
Macaca mulatta (Rhesus monkey) RPS17L XP_002804971.1
Canis lupus familiaris (dog) RPS17 NP_001003099.1
Bos taurus (cattle) RPS17 NP_001092680.1
Mus musculus (house mouse) Rps17 NP_033118.1
Gallus gallus (chicken) RPS17L NP_989548.1
Danio rerio (zebrafish) zgc:114188 NP_001013473.1
Caenorhabditis elegans rps-17 NP_491795.1
Arabidopsis thaliana (thale cress) AT3G10610 NP_187672.1
Arabidopsis thaliana (thale cress) AT5G04800 NP_001031834.1
Arabidopsis thaliana (thale cress) AT2G04390 NP_178520.1
Arabidopsis thaliana (thale cress) AT2G05220 NP_565320.1
Xenopus (Silurana) tropicalis (western clawed frog) rps17 XP_002938919.1

Component

ID Name Evidence
GO:0005622 intracellular IEA
GO:0005829 cytosol EXP
GO:0005829 cytosol TAS
GO:0005840 ribosome NAS
GO:0022627 cytosolic small ribosomal subunit IDA
GO:0022627 cytosolic small ribosomal subunit ISS

Function

ID Name Evidence
GO:0003735 structural constituent of ribosome NAS

Process

ID Name Evidence
GO:0000028 ribosomal small subunit assembly ISS
GO:0006364 rRNA processing IMP
GO:0006412 translation IC
GO:0006412 translation TAS
GO:0006413 translational initiation NAS
GO:0006414 translational elongation EXP
GO:0006414 translational elongation ISS
GO:0006415 translational termination TAS
GO:0010467 gene expression TAS
GO:0016032 viral reproduction TAS
GO:0019058 viral infectious cycle TAS
GO:0019083 viral transcription TAS
GO:0031018 endocrine pancreas development TAS
GO:0034101 erythrocyte homeostasis IMP
GO:0042274 ribosomal small subunit biogenesis IMP
GO:0044267 cellular protein metabolic process TAS

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RPS17 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPS17 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu23517
NM_001021 Homo sapiens ribosomal protein S17 (RPS17), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $49.50
$99.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu23517
Clone ID Related Accession (Same CDS sequence) NM_001021
Accession Version NM_001021.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 408bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 40S ribosomal protein S17
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BM017772.1, BC062715.1 and BE905890.1. On Apr 8, 2014 this sequence version replaced gi:71772428. Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S17E family of ribosomal proteins and is located in the cytoplasm. Mutations in this gene cause Diamond-Blackfan anemia 4. Alternative splicing of this gene results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Apr 2014]. Transcript Variant: This variant (1) encodes the supported protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI085304.1, BC062715.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
ATGGGCCGCG TTCGCACCAA AACCGTGAAG AAGGCGGCCC GGGTCATCAT AGAAAAGTAC 
TACACGCGCC TGGGCAACGA CTTCCACACG AACAAGCGCG TGTGCGAGGA GATCGCCATT
ATCCCCAGCA AAAAGCTCCG CAACAAGATA GCAGGTTATG TCACGCATCT GATGAAGCGA
ATTCAGAGAG GCCCAGTAAG AGGTATCTCC ATCAAGCTGC AGGAGGAGGA GAGAGAAAGG
AGAGACAATT ATGTTCCTGA GGTCTCAGCC TTGGATCAGG AGATTATTGA AGTAGATCCT
GACACTAAGG AAATGCTGAA GCTTTTGGAC TTCGGCAGTC TGTCCAACCT TCAGGTCACT
CAGCCTACAG TTGGGATGAA TTTCAAAACG CCTCGGGGAC CTGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001012.1
CDS117..524
Misc Feature(1)57..59(+)
Misc Feature(2)117..482(+)
Misc Feature(3)453..455(+)
Misc Feature(4)453..455(+)
Misc Feature(5)504..506(+)
Exon (1)1..119
Gene:RPS17
Gene Synonym:
Exon (2)120..271
Gene:RPS17
Gene Synonym:
Exon (3)272..377
Gene:RPS17
Gene Synonym:
Exon (4)378..443
Gene:RPS17
Gene Synonym:
Exon (5)444..575
Gene:RPS17
Gene Synonym:
Translation
Position Chain Variation Link
85 85 c, t dbSNP:575115395
88 88 c, g dbSNP:762051874
93 93 c, t dbSNP:751731453
94 94 c, t dbSNP:764270175
103 103 a, t dbSNP:763087945
110 110 c, t dbSNP:775421410
116 116 a, c dbSNP:770348460
118 118 g, t dbSNP:116840811
275 275 c, g, t dbSNP:6991
316 316 -, ga dbSNP:116840812

Target ORF information:

RefSeq Version NM_001021
Organism Homo sapiens (human)
Definition Homo sapiens ribosomal protein S17 (RPS17), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001021

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGGGCCGCG TTCGCACCAA AACCGTGAAG AAGGCGGCCC GGGTCATCAT AGAAAAGTAC 
TACACGCGCC TGGGCAACGA CTTCCACACG AACAAGCGCG TGTGCGAGGA GATCGCCATT
ATCCCCAGCA AAAAGCTCCG CAACAAGATA GCAGGTTATG TCACGCATCT GATGAAGCGA
ATTCAGAGAG GCCCAGTAAG AGGTATCTCC ATCAAGCTGC AGGAGGAGGA GAGAGAAAGG
AGAGACAATT ATGTTCCTGA GGTCTCAGCC TTGGATCAGG AGATTATTGA AGTAGATCCT
GACACTAAGG AAATGCTGAA GCTTTTGGAC TTCGGCAGTC TGTCCAACCT TCAGGTCACT
CAGCCTACAG TTGGGATGAA TTTCAAAACG CCTCGGGGAC CTGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Ribosomal protein mutations in Korean patients with Diamond-Blackfan anemia
Exp. Mol. Med. 46, E88 (2014)
Chae H, Park J, Lee S, Kim M, Kim Y, Lee JW, Chung NG, Cho B, Chul Jeong D, Kim J, Kim JR and Park G.


book

Adaptation of a genotype 3 hepatitis E virus to efficient growth in cell culture depends on an inserted human gene segment acquired by recombination
J. Virol. 86 (10), 5697-5707 (2012)
Shukla P, Nguyen HT, Faulk K, Mather K, Torian U, Engle RE and Emerson SU.


book

Extensive gene deletions in Japanese patients with Diamond-Blackfan anemia
Blood 119 (10), 2376-2384 (2012)
Kuramitsu M, Sato-Otsubo A, Morio T, Takagi M, Toki T, Terui K, Wang R, Kanno H, Ohga S, Ohara A, Kojima S, Kitoh T, Goi K, Kudo K, Matsubayashi T, Mizue N, Ozeki M, Masumi A, Momose H, Takizawa K, Mizukami T, Yamaguchi K, Ogawa S, Ito E and Hamaguchi I.


book

Ribosomal protein gene deletions in Diamond-Blackfan anemia
Blood 118 (26), 6943-6951 (2011)
Farrar JE, Vlachos A, Atsidaftos E, Carlson-Donohoe H, Markello TC, Arceci RJ, Ellis SR, Lipton JM and Bodine DM.


book

Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry
Eur. J. Biochem. 239 (1), 144-149 (1996)
Vladimirov SN, Ivanov AV, Karpova GG, Musolyamov AK, Egorov TA, Thiede B, Wittmann-Liebold B and Otto A.


book

Structural arrangement of the codon-anticodon interaction area in human placenta ribosomes. Affinity labelling of the 40S subunits by derivatives of oligoribonucleotides containing the AUG codon
Biochim. Biophys. Acta 1173 (3), 273-282 (1993)
Mundus DA, Bulygin KN, Yamkovoy VI, Malygin AA, Repkova MN, Vratskikh LV, Venijaminova AG, Vladimirov SN and Karpova GG.


book

Diamond-Blackfan Anemia
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Clinton,C. and Gazda,H.T.


book

The mapping of seven intron-containing ribosomal protein genes shows they are unlinked in the human genome
Genomics 13 (1), 201-207 (1992)
Feo S, Davies B and Fried M.


book

The transcriptionally active human ribosomal protein S17 gene
Gene 70 (1), 107-116 (1988)
Chen IT and Roufa DJ.


book

Homologous ribosomal proteins in bacteria, yeast, and humans
Proc. Natl. Acad. Sci. U.S.A. 83 (18), 6907-6911 (1986)
Chen,I.T., Dixit,A., Rhoads,D.D. and Roufa,D.J.


 
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