×

BDNF cDNA ORF clone, Homo sapiens (human)

Gene Symbol BDNF
Entrez Gene ID 627
Full Name brain-derived neurotrophic factor
Synonyms ANON2, BULN2
General protein information
Preferred Names
brain-derived neurotrophic factor
Names
brain-derived neurotrophic factor
abrineurin
neurotrophin
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p13

Summary The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jan 2009]. lac of sum
Disorder MIM:

113505

Disorder Html: {Memory impairment, susceptibility to} (3); Central hypoventilation

mRNA and Protein(s)

mRNA Protein Name
NM_001143809 NP_001137281 brain-derived neurotrophic factor isoform d preproprotein
NM_001143808 NP_001137280 brain-derived neurotrophic factor isoform a preproprotein
NM_001143807 NP_001137279 brain-derived neurotrophic factor isoform a preproprotein
NM_170735 NP_733931 brain-derived neurotrophic factor isoform a preproprotein
NM_170734 NP_733930 brain-derived neurotrophic factor isoform c preproprotein
XM_011520280 XP_011518582 brain-derived neurotrophic factor isoform X1
NM_170731 NP_733927 brain-derived neurotrophic factor isoform b preproprotein
NM_170732 NP_733928 brain-derived neurotrophic factor isoform a preproprotein
NM_170733 NP_733929 brain-derived neurotrophic factor isoform a preproprotein
NM_001709 NP_001700 brain-derived neurotrophic factor isoform a preproprotein
NM_001143805 NP_001137277 brain-derived neurotrophic factor isoform a preproprotein
NM_001143806 NP_001137278 brain-derived neurotrophic factor isoform a preproprotein
NM_001143810 NP_001137282 brain-derived neurotrophic factor isoform e
NM_001143811 NP_001137283 brain-derived neurotrophic factor isoform a preproprotein
NM_001143812 NP_001137284 brain-derived neurotrophic factor isoform a preproprotein
NM_001143813 NP_001137285 brain-derived neurotrophic factor isoform a preproprotein
NM_001143814 NP_001137286 brain-derived neurotrophic factor isoform a preproprotein
NM_001143816 NP_001137288 brain-derived neurotrophic factor isoform a preproprotein



Homo sapiens (human) BDNF NP_001137282.1
Pan troglodytes (chimpanzee) BDNF NP_001012443.1
Macaca mulatta (Rhesus monkey) BDNF XP_001089568.2
Canis lupus familiaris (dog) BDNF NP_001002975.1
Bos taurus (cattle) BDNF NP_001040072.1
Mus musculus (house mouse) Bdnf NP_031566.4
Rattus norvegicus (Norway rat) Bdnf NP_036645.2
Gallus gallus (chicken) BDNF NP_001026787.1
Danio rerio (zebrafish) bdnf NP_571670.2
Xenopus (Silurana) tropicalis (western clawed frog) bdnf NP_001090767.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BDNF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BDNF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu27396 NM_001143809 Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 17, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu26594
NM_001143808 Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu26594
NM_001143807 Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu26594
NM_170735 Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu26730
NM_170734 Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu26743 XM_011520280 PREDICTED: Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu27197
NM_170731 Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu26594
NM_170732 Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu26594
NM_170733 Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu26594
NM_001709 Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu26594
NM_001143805 Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu26594
NM_001143806 Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu26743 NM_001143810 Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 18, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu26594
NM_001143811 Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu26594
NM_001143812 Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu26594
NM_001143813 Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu26594
NM_001143814 Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 14, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu26594
NM_001143816 Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 16, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu27396
Clone ID Related Accession (Same CDS sequence) NM_001143809
Accession Version NM_001143809.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 831bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-MAY-2014
Organism Homo sapiens (human)
Product brain-derived neurotrophic factor isoform d preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF689012.1. Transcript Variant: This variant (17), also known as Vb, represents use of an alternate promoter and differs in the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (d) contains a longer N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF689012.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCTCTGTG CGATTTCATT GTGTGCTCGC GTTCGCAAGC TCCGTAGTGC AGGAAGGTGC 
GGGAAGTTCC ACCAGGTGAG AAGAGTGATG ACCATCCTTT TCCTTACTAT GGTTATTTCA
TACTTTGGTT GCATGAAGGC TGCCCCCATG AAAGAAGCAA ACATCCGAGG ACAAGGTGGC
TTGGCCTACC CAGGTGTGCG GACCCATGGG ACTCTGGAGA GCGTGAATGG GCCCAAGGCA
GGTTCAAGAG GCTTGACATC ATTGGCTGAC ACTTTCGAAC ACGTGATAGA AGAGCTGTTG
GATGAGGACC AGAAAGTTCG GCCCAATGAA GAAAACAATA AGGACGCAGA CTTGTACACG
TCCAGGGTGA TGCTCAGTAG TCAAGTGCCT TTGGAGCCTC CTCTTCTCTT TCTGCTGGAG
GAATACAAAA ATTACCTAGA TGCTGCAAAC ATGTCCATGA GGGTCCGGCG CCACTCTGAC
CCTGCCCGCC GAGGGGAGCT GAGCGTGTGT GACAGTATTA GTGAGTGGGT AACGGCGGCA
GACAAAAAGA CTGCAGTGGA CATGTCGGGC GGGACGGTCA CAGTCCTTGA AAAGGTCCCT
GTATCAAAAG GCCAACTGAA GCAATACTTC TACGAGACCA AGTGCAATCC CATGGGTTAC
ACAAAAGAAG GCTGCAGGGG CATAGACAAA AGGCATTGGA ACTCCCAGTG CCGAACTACC
CAGTCGTACG TGCGGGCCCT TACCATGGAT AGCAAAAAGA GAATTGGCTG GCGATTCATA
AGGATAGACA CTTCTTGTGT ATGTACATTG ACCATTAAAA GGGGAAGATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001137281.1
CDS18..848
Misc Feature(1)3..3(+)
Misc Feature(2)4..4(+)
Misc Feature(3)492..845(+)
Exon (1)1..83
Gene:BDNF
Gene Synonym:
Exon (2)84..3774
Gene:BDNF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001143809
Organism Homo sapiens (human)
Definition Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 17, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001143809

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCTCTGTG CGATTTCATT GTGTGCTCGC GTTCGCAAGC TCCGTAGTGC AGGAAGGTGC 
GGGAAGTTCC ACCAGGTGAG AAGAGTGATG ACCATCCTTT TCCTTACTAT GGTTATTTCA
TACTTTGGTT GCATGAAGGC TGCCCCCATG AAAGAAGCAA ACATCCGAGG ACAAGGTGGC
TTGGCCTACC CAGGTGTGCG GACCCATGGG ACTCTGGAGA GCGTGAATGG GCCCAAGGCA
GGTTCAAGAG GCTTGACATC ATTGGCTGAC ACTTTCGAAC ACGTGATAGA AGAGCTGTTG
GATGAGGACC AGAAAGTTCG GCCCAATGAA GAAAACAATA AGGACGCAGA CTTGTACACG
TCCAGGGTGA TGCTCAGTAG TCAAGTGCCT TTGGAGCCTC CTCTTCTCTT TCTGCTGGAG
GAATACAAAA ATTACCTAGA TGCTGCAAAC ATGTCCATGA GGGTCCGGCG CCACTCTGAC
CCTGCCCGCC GAGGGGAGCT GAGCGTGTGT GACAGTATTA GTGAGTGGGT AACGGCGGCA
GACAAAAAGA CTGCAGTGGA CATGTCGGGC GGGACGGTCA CAGTCCTTGA AAAGGTCCCT
GTATCAAAAG GCCAACTGAA GCAATACTTC TACGAGACCA AGTGCAATCC CATGGGTTAC
ACAAAAGAAG GCTGCAGGGG CATAGACAAA AGGCATTGGA ACTCCCAGTG CCGAACTACC
CAGTCGTACG TGCGGGCCCT TACCATGGAT AGCAAAAAGA GAATTGGCTG GCGATTCATA
AGGATAGACA CTTCTTGTGT ATGTACATTG ACCATTAAAA GGGGAAGATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26594
Clone ID Related Accession (Same CDS sequence) NM_001143805 , NM_001143806 , NM_001143807 , NM_001143808 , NM_001143811 , NM_001143812 , NM_001143813 , NM_001143814 , NM_001143816 , NM_001709 , NM_170732 , NM_170733 , NM_170735
Accession Version NM_001143808.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 744bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-MAY-2014
Organism Homo sapiens (human)
Product brain-derived neurotrophic factor isoform a preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF689011.1. Transcript Variant: This variant (10), also known as Va, differs in the 5' UTR and represents use of an alternate promoter compared to variant 1. Variants 1, 2, 4, 5, and 7-16 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF689011.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001137280.1
CDS95..838
Misc Feature(1)3..3(+)
Misc Feature(2)4..4(+)
Misc Feature(3)62..64(+)
Misc Feature(4)263..268(+)
Misc Feature(5)482..835(+)
Exon (1)1..73
Gene:BDNF
Gene Synonym:
Exon (2)74..3764
Gene:BDNF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001143808
Organism Homo sapiens (human)
Definition Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001143808

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26594
Clone ID Related Accession (Same CDS sequence) NM_001143805 , NM_001143806 , NM_001143807 , NM_001143808 , NM_001143811 , NM_001143812 , NM_001143813 , NM_001143814 , NM_001143816 , NM_001709 , NM_170732 , NM_170733 , NM_170735
Accession Version NM_001143807.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 744bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-MAY-2014
Organism Homo sapiens (human)
Product brain-derived neurotrophic factor isoform a preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF674520.1. Transcript Variant: This variant (9), also known as III or BDNF3, differs in the 5' UTR and represents use of an alternate promoter compared to variant 1. Variants 1, 2, 4, 5, and 7-16 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF674520.1, AY054396.2 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001137279.1
CDS259..1002
Misc Feature(1)46..46(+)
Misc Feature(2)229..231(+)
Misc Feature(3)427..432(+)
Misc Feature(4)646..999(+)
Exon (1)1..237
Gene:BDNF
Gene Synonym:
Exon (2)238..3928
Gene:BDNF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001143807
Organism Homo sapiens (human)
Definition Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001143807

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26594
Clone ID Related Accession (Same CDS sequence) NM_001143805 , NM_001143806 , NM_001143807 , NM_001143808 , NM_001143811 , NM_001143812 , NM_001143813 , NM_001143814 , NM_001143816 , NM_001709 , NM_170732 , NM_170733 , NM_170735
Accession Version NM_170735.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 744bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 26-MAY-2014
Organism Homo sapiens (human)
Product brain-derived neurotrophic factor isoform a preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF689009.1. On Jan 9, 2009 this sequence version replaced gi:60218885. Transcript Variant: This variant (1), also known as IX, represents the longest transcript. Variants 1, 2, 4, 5, and 7-16 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: EF689009.1 [ECO:0000345] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_733931.1
CDS1086..1829
Misc Feature(1)1026..1028(+)
Misc Feature(2)1254..1259(+)
Misc Feature(3)1254..1256(+)
Misc Feature(4)1473..1826(+)
Exon (1)1..4755
Gene:BDNF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_170735
Organism Homo sapiens (human)
Definition Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_170735

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26730
Clone ID Related Accession (Same CDS sequence) NM_170734
Accession Version NM_170734.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 789bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-MAY-2014
Organism Homo sapiens (human)
Product brain-derived neurotrophic factor isoform c preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF689018.1. On Jan 9, 2009 this sequence version replaced gi:34106709. Transcript Variant: This variant (6), also known as VIIb, BDNF6B, or 4I-5, represents use of an alternate promoter and differs in the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (c) contains a longer N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF689018.1, AB038674.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCAGAGCC GGGAAGAGGA ATGGTTCCAC CAGGTGAGAA GAGTGATGAC CATCCTTTTC 
CTTACTATGG TTATTTCATA CTTTGGTTGC ATGAAGGCTG CCCCCATGAA AGAAGCAAAC
ATCCGAGGAC AAGGTGGCTT GGCCTACCCA GGTGTGCGGA CCCATGGGAC TCTGGAGAGC
GTGAATGGGC CCAAGGCAGG TTCAAGAGGC TTGACATCAT TGGCTGACAC TTTCGAACAC
GTGATAGAAG AGCTGTTGGA TGAGGACCAG AAAGTTCGGC CCAATGAAGA AAACAATAAG
GACGCAGACT TGTACACGTC CAGGGTGATG CTCAGTAGTC AAGTGCCTTT GGAGCCTCCT
CTTCTCTTTC TGCTGGAGGA ATACAAAAAT TACCTAGATG CTGCAAACAT GTCCATGAGG
GTCCGGCGCC ACTCTGACCC TGCCCGCCGA GGGGAGCTGA GCGTGTGTGA CAGTATTAGT
GAGTGGGTAA CGGCGGCAGA CAAAAAGACT GCAGTGGACA TGTCGGGCGG GACGGTCACA
GTCCTTGAAA AGGTCCCTGT ATCAAAAGGC CAACTGAAGC AATACTTCTA CGAGACCAAG
TGCAATCCCA TGGGTTACAC AAAAGAAGGC TGCAGGGGCA TAGACAAAAG GCATTGGAAC
TCCCAGTGCC GAACTACCCA GTCGTACGTG CGGGCCCTTA CCATGGATAG CAAAAAGAGA
ATTGGCTGGC GATTCATAAG GATAGACACT TCTTGTGTAT GTACATTGAC CATTAAAAGG
GGAAGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_733930.1
CDS262..1050
Misc Feature(1)250..252(+)
Misc Feature(2)430..432(+)
Misc Feature(3)694..1047(+)
Exon (1)1..285
Gene:BDNF
Gene Synonym:
Exon (2)286..3976
Gene:BDNF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_170734
Organism Homo sapiens (human)
Definition Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_170734

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCAGAGCC GGGAAGAGGA ATGGTTCCAC CAGGTGAGAA GAGTGATGAC CATCCTTTTC 
CTTACTATGG TTATTTCATA CTTTGGTTGC ATGAAGGCTG CCCCCATGAA AGAAGCAAAC
ATCCGAGGAC AAGGTGGCTT GGCCTACCCA GGTGTGCGGA CCCATGGGAC TCTGGAGAGC
GTGAATGGGC CCAAGGCAGG TTCAAGAGGC TTGACATCAT TGGCTGACAC TTTCGAACAC
GTGATAGAAG AGCTGTTGGA TGAGGACCAG AAAGTTCGGC CCAATGAAGA AAACAATAAG
GACGCAGACT TGTACACGTC CAGGGTGATG CTCAGTAGTC AAGTGCCTTT GGAGCCTCCT
CTTCTCTTTC TGCTGGAGGA ATACAAAAAT TACCTAGATG CTGCAAACAT GTCCATGAGG
GTCCGGCGCC ACTCTGACCC TGCCCGCCGA GGGGAGCTGA GCGTGTGTGA CAGTATTAGT
GAGTGGGTAA CGGCGGCAGA CAAAAAGACT GCAGTGGACA TGTCGGGCGG GACGGTCACA
GTCCTTGAAA AGGTCCCTGT ATCAAAAGGC CAACTGAAGC AATACTTCTA CGAGACCAAG
TGCAATCCCA TGGGTTACAC AAAAGAAGGC TGCAGGGGCA TAGACAAAAG GCATTGGAAC
TCCCAGTGCC GAACTACCCA GTCGTACGTG CGGGCCCTTA CCATGGATAG CAAAAAGAGA
ATTGGCTGGC GATTCATAAG GATAGACACT TCTTGTGTAT GTACATTGAC CATTAAAAGG
GGAAGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26743
Clone ID Related Accession (Same CDS sequence) NM_001143810 , XM_011520280
Accession Version XM_011520280.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 990bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product brain-derived neurotrophic factor isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTGTGGAG CCACCAGTTT TCTCCATGAG TGCACAAGGT TAATCCTTGT TACTACTCAG 
AATGCTGAGT TTCTACAGAA AGGGTTGCAG GTCCACACAT GTTTTGGCGT CTACCCACAC
GCTTCTGTAT GGCATGACTG TGCATCCCAG AAGAAGGGCT GTGCTGTGTA CCTCCACGTT
TCAGTGGAAT TTAACAAACT GATCCCTGAA AATGGTTTCA TAAAGTTCCA CCAGGTGAGA
AGAGTGATGA CCATCCTTTT CCTTACTATG GTTATTTCAT ACTTTGGTTG CATGAAGGCT
GCCCCCATGA AAGAAGCAAA CATCCGAGGA CAAGGTGGCT TGGCCTACCC AGGTGTGCGG
ACCCATGGGA CTCTGGAGAG CGTGAATGGG CCCAAGGCAG GTTCAAGAGG CTTGACATCA
TTGGCTGACA CTTTCGAACA CGTGATAGAA GAGCTGTTGG ATGAGGACCA GAAAGTTCGG
CCCAATGAAG AAAACAATAA GGACGCAGAC TTGTACACGT CCAGGGTGAT GCTCAGTAGT
CAAGTGCCTT TGGAGCCTCC TCTTCTCTTT CTGCTGGAGG AATACAAAAA TTACCTAGAT
GCTGCAAACA TGTCCATGAG GGTCCGGCGC CACTCTGACC CTGCCCGCCG AGGGGAGCTG
AGCGTGTGTG ACAGTATTAG TGAGTGGGTA ACGGCGGCAG ACAAAAAGAC TGCAGTGGAC
ATGTCGGGCG GGACGGTCAC AGTCCTTGAA AAGGTCCCTG TATCAAAAGG CCAACTGAAG
CAATACTTCT ACGAGACCAA GTGCAATCCC ATGGGTTACA CAAAAGAAGG CTGCAGGGGC
ATAGACAAAA GGCATTGGAA CTCCCAGTGC CGAACTACCC AGTCGTACGT GCGGGCCCTT
ACCATGGATA GCAAAAAGAG AATTGGCTGG CGATTCATAA GGATAGACAC TTCTTGTGTA
TGTACATTGA CCATTAAAAG GGGAAGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011518582.1
CDS1006..1995
Misc Feature(1)1639..1992(+)
Translation

Target ORF information:

RefSeq Version XM_011520280
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011520280

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTGTGGAG CCACCAGTTT TCTCCATGAG TGCACAAGGT TAATCCTTGT TACTACTCAG 
AATGCTGAGT TTCTACAGAA AGGGTTGCAG GTCCACACAT GTTTTGGCGT CTACCCACAC
GCTTCTGTAT GGCATGACTG TGCATCCCAG AAGAAGGGCT GTGCTGTGTA CCTCCACGTT
TCAGTGGAAT TTAACAAACT GATCCCTGAA AATGGTTTCA TAAAGTTCCA CCAGGTGAGA
AGAGTGATGA CCATCCTTTT CCTTACTATG GTTATTTCAT ACTTTGGTTG CATGAAGGCT
GCCCCCATGA AAGAAGCAAA CATCCGAGGA CAAGGTGGCT TGGCCTACCC AGGTGTGCGG
ACCCATGGGA CTCTGGAGAG CGTGAATGGG CCCAAGGCAG GTTCAAGAGG CTTGACATCA
TTGGCTGACA CTTTCGAACA CGTGATAGAA GAGCTGTTGG ATGAGGACCA GAAAGTTCGG
CCCAATGAAG AAAACAATAA GGACGCAGAC TTGTACACGT CCAGGGTGAT GCTCAGTAGT
CAAGTGCCTT TGGAGCCTCC TCTTCTCTTT CTGCTGGAGG AATACAAAAA TTACCTAGAT
GCTGCAAACA TGTCCATGAG GGTCCGGCGC CACTCTGACC CTGCCCGCCG AGGGGAGCTG
AGCGTGTGTG ACAGTATTAG TGAGTGGGTA ACGGCGGCAG ACAAAAAGAC TGCAGTGGAC
ATGTCGGGCG GGACGGTCAC AGTCCTTGAA AAGGTCCCTG TATCAAAAGG CCAACTGAAG
CAATACTTCT ACGAGACCAA GTGCAATCCC ATGGGTTACA CAAAAGAAGG CTGCAGGGGC
ATAGACAAAA GGCATTGGAA CTCCCAGTGC CGAACTACCC AGTCGTACGT GCGGGCCCTT
ACCATGGATA GCAAAAAGAG AATTGGCTGG CGATTCATAA GGATAGACAC TTCTTGTGTA
TGTACATTGA CCATTAAAAG GGGAAGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27197
Clone ID Related Accession (Same CDS sequence) NM_170731
Accession Version NM_170731.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 768bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product brain-derived neurotrophic factor isoform b preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF689021.1. This sequence is a reference standard in the RefSeqGene project. On Jan 9, 2009 this sequence version replaced gi:60218883. Transcript Variant: This variant (3), also known as I, BDNF1, or 1-5, represents use of an alternate promoter and differs in the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (b) contains a longer N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF689021.1, AK289853.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTTCCACC AGGTGAGAAG AGTGATGACC ATCCTTTTCC TTACTATGGT TATTTCATAC 
TTTGGTTGCA TGAAGGCTGC CCCCATGAAA GAAGCAAACA TCCGAGGACA AGGTGGCTTG
GCCTACCCAG GTGTGCGGAC CCATGGGACT CTGGAGAGCG TGAATGGGCC CAAGGCAGGT
TCAAGAGGCT TGACATCATT GGCTGACACT TTCGAACACG TGATAGAAGA GCTGTTGGAT
GAGGACCAGA AAGTTCGGCC CAATGAAGAA AACAATAAGG ACGCAGACTT GTACACGTCC
AGGGTGATGC TCAGTAGTCA AGTGCCTTTG GAGCCTCCTC TTCTCTTTCT GCTGGAGGAA
TACAAAAATT ACCTAGATGC TGCAAACATG TCCATGAGGG TCCGGCGCCA CTCTGACCCT
GCCCGCCGAG GGGAGCTGAG CGTGTGTGAC AGTATTAGTG AGTGGGTAAC GGCGGCAGAC
AAAAAGACTG CAGTGGACAT GTCGGGCGGG ACGGTCACAG TCCTTGAAAA GGTCCCTGTA
TCAAAAGGCC AACTGAAGCA ATACTTCTAC GAGACCAAGT GCAATCCCAT GGGTTACACA
AAAGAAGGCT GCAGGGGCAT AGACAAAAGG CATTGGAACT CCCAGTGCCG AACTACCCAG
TCGTACGTGC GGGCCCTTAC CATGGATAGC AAAAAGAGAA TTGGCTGGCG ATTCATAAGG
ATAGACACTT CTTGTGTATG TACATTGACC ATTAAAAGGG GAAGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_733927.1
CDS645..1412
Misc Feature(1)219..219(+)
Misc Feature(2)531..533(+)
Misc Feature(3)837..839(+)
Misc Feature(4)1056..1409(+)
Exon (1)1..647
Gene:BDNF
Gene Synonym:
Exon (2)648..4338
Gene:BDNF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_170731
Organism Homo sapiens (human)
Definition Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_170731

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTTCCACC AGGTGAGAAG AGTGATGACC ATCCTTTTCC TTACTATGGT TATTTCATAC 
TTTGGTTGCA TGAAGGCTGC CCCCATGAAA GAAGCAAACA TCCGAGGACA AGGTGGCTTG
GCCTACCCAG GTGTGCGGAC CCATGGGACT CTGGAGAGCG TGAATGGGCC CAAGGCAGGT
TCAAGAGGCT TGACATCATT GGCTGACACT TTCGAACACG TGATAGAAGA GCTGTTGGAT
GAGGACCAGA AAGTTCGGCC CAATGAAGAA AACAATAAGG ACGCAGACTT GTACACGTCC
AGGGTGATGC TCAGTAGTCA AGTGCCTTTG GAGCCTCCTC TTCTCTTTCT GCTGGAGGAA
TACAAAAATT ACCTAGATGC TGCAAACATG TCCATGAGGG TCCGGCGCCA CTCTGACCCT
GCCCGCCGAG GGGAGCTGAG CGTGTGTGAC AGTATTAGTG AGTGGGTAAC GGCGGCAGAC
AAAAAGACTG CAGTGGACAT GTCGGGCGGG ACGGTCACAG TCCTTGAAAA GGTCCCTGTA
TCAAAAGGCC AACTGAAGCA ATACTTCTAC GAGACCAAGT GCAATCCCAT GGGTTACACA
AAAGAAGGCT GCAGGGGCAT AGACAAAAGG CATTGGAACT CCCAGTGCCG AACTACCCAG
TCGTACGTGC GGGCCCTTAC CATGGATAGC AAAAAGAGAA TTGGCTGGCG ATTCATAAGG
ATAGACACTT CTTGTGTATG TACATTGACC ATTAAAAGGG GAAGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26594
Clone ID Related Accession (Same CDS sequence) NM_001143805 , NM_001143806 , NM_001143807 , NM_001143808 , NM_001143811 , NM_001143812 , NM_001143813 , NM_001143814 , NM_001143816 , NM_001709 , NM_170732 , NM_170733 , NM_170735
Accession Version NM_170732.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 744bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product brain-derived neurotrophic factor isoform a preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF674519.1. On Jan 9, 2009 this sequence version replaced gi:60218884. Transcript Variant: This variant (2), also known as IIc, BDNF2C, or 2-5, differs in the 5' UTR and represents use of an alternate promoter compared to variant 1. Variants 1, 2, 4, 5, and 7-16 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF674519.1, AK289763.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_733928.1
CDS456..1199
Misc Feature(1)11..11(+)
Misc Feature(2)12..12(+)
Misc Feature(3)18..18(+)
Misc Feature(4)27..27(+)
Misc Feature(5)34..34(+)
Misc Feature(6)208..208(+)
Misc Feature(7)210..210(+)
Misc Feature(8)230..230(+)
Misc Feature(9)234..234(+)
Misc Feature(10)356..356(+)
Misc Feature(11)387..387(+)
Misc Feature(12)420..422(+)
Misc Feature(13)624..629(+)
Misc Feature(14)624..626(+)
Misc Feature(15)843..1196(+)
Exon (1)1..434
Gene:BDNF
Gene Synonym:
Exon (2)435..4125
Gene:BDNF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_170732
Organism Homo sapiens (human)
Definition Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_170732

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26594
Clone ID Related Accession (Same CDS sequence) NM_001143805 , NM_001143806 , NM_001143807 , NM_001143808 , NM_001143811 , NM_001143812 , NM_001143813 , NM_001143814 , NM_001143816 , NM_001709 , NM_170732 , NM_170733 , NM_170735
Accession Version NM_170733.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 744bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product brain-derived neurotrophic factor isoform a preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF674521.1. On Jan 9, 2009 this sequence version replaced gi:34106708. Transcript Variant: This variant (5), also known as IV, BDNF4, or 3-5, differs in the 5' UTR and represents use of an alternate promoter compared to variant 1. Variants 1, 2, 4, 5, and 7-16 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF674521.1, BC029795.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_733929.1
CDS359..1102
Misc Feature(1)5..5(+)
Misc Feature(2)64..64(+)
Misc Feature(3)123..123(+)
Misc Feature(4)317..319(+)
Misc Feature(5)527..532(+)
Misc Feature(6)527..529(+)
Misc Feature(7)746..1099(+)
Exon (1)1..337
Gene:BDNF
Gene Synonym:
Exon (2)338..4028
Gene:BDNF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_170733
Organism Homo sapiens (human)
Definition Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_170733

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26594
Clone ID Related Accession (Same CDS sequence) NM_001143805 , NM_001143806 , NM_001143807 , NM_001143808 , NM_001143811 , NM_001143812 , NM_001143813 , NM_001143814 , NM_001143816 , NM_001709 , NM_170732 , NM_170733 , NM_170735
Accession Version NM_001709.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 744bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product brain-derived neurotrophic factor isoform a preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF689015.1. On Jan 9, 2009 this sequence version replaced gi:34106711. Transcript Variant: This variant (4), also known as VIb or BDNF5, differs in the 5' UTR and represents use of an alternate promoter compared to variant 1. Variants 1, 2, 4, 5, and 7-16 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF689015.1, X60201.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158569 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001700.2
CDS347..1090
Misc Feature(1)2..2(+)
Misc Feature(2)6..6(+)
Misc Feature(3)7..7(+)
Misc Feature(4)11..11(+)
Misc Feature(5)17..19(+)
Misc Feature(6)515..520(+)
Misc Feature(7)515..517(+)
Misc Feature(8)734..1087(+)
Exon (1)1..325
Gene:BDNF
Gene Synonym:
Exon (2)326..4016
Gene:BDNF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001709
Organism Homo sapiens (human)
Definition Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001709

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26594
Clone ID Related Accession (Same CDS sequence) NM_001143805 , NM_001143806 , NM_001143807 , NM_001143808 , NM_001143811 , NM_001143812 , NM_001143813 , NM_001143814 , NM_001143816 , NM_001709 , NM_170732 , NM_170733 , NM_170735
Accession Version NM_001143805.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 744bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product brain-derived neurotrophic factor isoform a preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF674517.1. Transcript Variant: This variant (7), also known as IIa or BDNF2A, differs in the 5' UTR and represents use of an alternate promoter compared to variant 1. Variants 1, 2, 4, 5, and 7-16 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF674517.1, AY054393.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001137277.1
CDS158..901
Misc Feature(1)11..11(+)
Misc Feature(2)12..12(+)
Misc Feature(3)18..18(+)
Misc Feature(4)27..27(+)
Misc Feature(5)34..34(+)
Misc Feature(6)74..76(+)
Misc Feature(7)326..331(+)
Misc Feature(8)545..898(+)
Exon (1)1..136
Gene:BDNF
Gene Synonym:
Exon (2)137..3827
Gene:BDNF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001143805
Organism Homo sapiens (human)
Definition Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001143805

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26594
Clone ID Related Accession (Same CDS sequence) NM_001143805 , NM_001143806 , NM_001143807 , NM_001143808 , NM_001143811 , NM_001143812 , NM_001143813 , NM_001143814 , NM_001143816 , NM_001709 , NM_170732 , NM_170733 , NM_170735
Accession Version NM_001143806.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 744bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product brain-derived neurotrophic factor isoform a preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF674518.1. Transcript Variant: This variant (8), also known as IIb or BDNF2B, differs in the 5' UTR and represents use of an alternate promoter compared to variant 1. Variants 1, 2, 4, 5, and 7-16 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF674518.1, AY054394.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001137278.1
CDS373..1116
Misc Feature(1)11..11(+)
Misc Feature(2)12..12(+)
Misc Feature(3)18..18(+)
Misc Feature(4)27..27(+)
Misc Feature(5)34..34(+)
Misc Feature(6)208..208(+)
Misc Feature(7)210..210(+)
Misc Feature(8)230..230(+)
Misc Feature(9)234..234(+)
Misc Feature(10)331..333(+)
Misc Feature(11)541..546(+)
Misc Feature(12)760..1113(+)
Exon (1)1..351
Gene:BDNF
Gene Synonym:
Exon (2)352..4042
Gene:BDNF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001143806
Organism Homo sapiens (human)
Definition Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001143806

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26743
Clone ID Related Accession (Same CDS sequence) NM_001143810 , XM_011520280
Accession Version NM_001143810.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 990bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product brain-derived neurotrophic factor isoform e
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF689019.1. Transcript Variant: This variant (18), also known as V-VIII, represents use of an alternate promoter and differs in the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (e) contains a longer N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF689019.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2145743, SAMEA2145893 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTGTGGAG CCACCAGTTT TCTCCATGAG TGCACAAGGT TAATCCTTGT TACTACTCAG 
AATGCTGAGT TTCTACAGAA AGGGTTGCAG GTCCACACAT GTTTTGGCGT CTACCCACAC
GCTTCTGTAT GGCATGACTG TGCATCCCAG AAGAAGGGCT GTGCTGTGTA CCTCCACGTT
TCAGTGGAAT TTAACAAACT GATCCCTGAA AATGGTTTCA TAAAGTTCCA CCAGGTGAGA
AGAGTGATGA CCATCCTTTT CCTTACTATG GTTATTTCAT ACTTTGGTTG CATGAAGGCT
GCCCCCATGA AAGAAGCAAA CATCCGAGGA CAAGGTGGCT TGGCCTACCC AGGTGTGCGG
ACCCATGGGA CTCTGGAGAG CGTGAATGGG CCCAAGGCAG GTTCAAGAGG CTTGACATCA
TTGGCTGACA CTTTCGAACA CGTGATAGAA GAGCTGTTGG ATGAGGACCA GAAAGTTCGG
CCCAATGAAG AAAACAATAA GGACGCAGAC TTGTACACGT CCAGGGTGAT GCTCAGTAGT
CAAGTGCCTT TGGAGCCTCC TCTTCTCTTT CTGCTGGAGG AATACAAAAA TTACCTAGAT
GCTGCAAACA TGTCCATGAG GGTCCGGCGC CACTCTGACC CTGCCCGCCG AGGGGAGCTG
AGCGTGTGTG ACAGTATTAG TGAGTGGGTA ACGGCGGCAG ACAAAAAGAC TGCAGTGGAC
ATGTCGGGCG GGACGGTCAC AGTCCTTGAA AAGGTCCCTG TATCAAAAGG CCAACTGAAG
CAATACTTCT ACGAGACCAA GTGCAATCCC ATGGGTTACA CAAAAGAAGG CTGCAGGGGC
ATAGACAAAA GGCATTGGAA CTCCCAGTGC CGAACTACCC AGTCGTACGT GCGGGCCCTT
ACCATGGATA GCAAAAAGAG AATTGGCTGG CGATTCATAA GGATAGACAC TTCTTGTGTA
TGTACATTGA CCATTAAAAG GGGAAGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001137282.1
CDS142..1131
Misc Feature(1)3..3(+)
Misc Feature(2)4..4(+)
Misc Feature(3)85..87(+)
Misc Feature(4)775..1128(+)
Exon (1)1..83
Gene:BDNF
Gene Synonym:
Exon (2)84..366
Gene:BDNF
Gene Synonym:
Exon (3)367..4057
Gene:BDNF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001143810
Organism Homo sapiens (human)
Definition Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 18, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001143810

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGTGTGGAG CCACCAGTTT TCTCCATGAG TGCACAAGGT TAATCCTTGT TACTACTCAG 
AATGCTGAGT TTCTACAGAA AGGGTTGCAG GTCCACACAT GTTTTGGCGT CTACCCACAC
GCTTCTGTAT GGCATGACTG TGCATCCCAG AAGAAGGGCT GTGCTGTGTA CCTCCACGTT
TCAGTGGAAT TTAACAAACT GATCCCTGAA AATGGTTTCA TAAAGTTCCA CCAGGTGAGA
AGAGTGATGA CCATCCTTTT CCTTACTATG GTTATTTCAT ACTTTGGTTG CATGAAGGCT
GCCCCCATGA AAGAAGCAAA CATCCGAGGA CAAGGTGGCT TGGCCTACCC AGGTGTGCGG
ACCCATGGGA CTCTGGAGAG CGTGAATGGG CCCAAGGCAG GTTCAAGAGG CTTGACATCA
TTGGCTGACA CTTTCGAACA CGTGATAGAA GAGCTGTTGG ATGAGGACCA GAAAGTTCGG
CCCAATGAAG AAAACAATAA GGACGCAGAC TTGTACACGT CCAGGGTGAT GCTCAGTAGT
CAAGTGCCTT TGGAGCCTCC TCTTCTCTTT CTGCTGGAGG AATACAAAAA TTACCTAGAT
GCTGCAAACA TGTCCATGAG GGTCCGGCGC CACTCTGACC CTGCCCGCCG AGGGGAGCTG
AGCGTGTGTG ACAGTATTAG TGAGTGGGTA ACGGCGGCAG ACAAAAAGAC TGCAGTGGAC
ATGTCGGGCG GGACGGTCAC AGTCCTTGAA AAGGTCCCTG TATCAAAAGG CCAACTGAAG
CAATACTTCT ACGAGACCAA GTGCAATCCC ATGGGTTACA CAAAAGAAGG CTGCAGGGGC
ATAGACAAAA GGCATTGGAA CTCCCAGTGC CGAACTACCC AGTCGTACGT GCGGGCCCTT
ACCATGGATA GCAAAAAGAG AATTGGCTGG CGATTCATAA GGATAGACAC TTCTTGTGTA
TGTACATTGA CCATTAAAAG GGGAAGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26594
Clone ID Related Accession (Same CDS sequence) NM_001143805 , NM_001143806 , NM_001143807 , NM_001143808 , NM_001143811 , NM_001143812 , NM_001143813 , NM_001143814 , NM_001143816 , NM_001709 , NM_170732 , NM_170733 , NM_170735
Accession Version NM_001143811.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 744bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product brain-derived neurotrophic factor isoform a preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF689020.1. Transcript Variant: This variant (11), also known as V-VIII-VIIIh, differs in the 5' UTR and represents use of an alternate promoter compared to variant 1. Variants 1, 2, 4, 5, and 7-16 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF689020.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2158188 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001137283.1
CDS505..1248
Misc Feature(1)3..3(+)
Misc Feature(2)4..4(+)
Misc Feature(3)475..477(+)
Misc Feature(4)673..678(+)
Misc Feature(5)892..1245(+)
Exon (1)1..83
Gene:BDNF
Gene Synonym:
Exon (2)84..366
Gene:BDNF
Gene Synonym:
Exon (3)367..483
Gene:BDNF
Gene Synonym:
Exon (4)484..4174
Gene:BDNF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001143811
Organism Homo sapiens (human)
Definition Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001143811

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26594
Clone ID Related Accession (Same CDS sequence) NM_001143805 , NM_001143806 , NM_001143807 , NM_001143808 , NM_001143811 , NM_001143812 , NM_001143813 , NM_001143814 , NM_001143816 , NM_001709 , NM_170732 , NM_170733 , NM_170735
Accession Version NM_001143812.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 744bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product brain-derived neurotrophic factor isoform a preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF689013.1 and CX751420.1. Transcript Variant: This variant (12), also known as Vh, differs in the 5' UTR and represents use of an alternate promoter compared to variant 1. Variants 1, 2, 4, 5, and 7-16 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF689013.1, CX751420.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001137284.1
CDS247..990
Misc Feature(1)13..15(+)
Misc Feature(2)415..420(+)
Misc Feature(3)634..987(+)
Exon (1)1..225
Gene:BDNF
Gene Synonym:
Exon (2)226..3916
Gene:BDNF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001143812
Organism Homo sapiens (human)
Definition Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001143812

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26594
Clone ID Related Accession (Same CDS sequence) NM_001143805 , NM_001143806 , NM_001143807 , NM_001143808 , NM_001143811 , NM_001143812 , NM_001143813 , NM_001143814 , NM_001143816 , NM_001709 , NM_170732 , NM_170733 , NM_170735
Accession Version NM_001143813.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 744bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product brain-derived neurotrophic factor isoform a preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF689014.1. Transcript Variant: This variant (13), also known as VIa, differs in the 5' UTR and represents use of an alternate promoter compared to variant 1. Variants 1, 2, 4, 5, and 7-16 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF689014.1, BU506938.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001137285.1
CDS329..1072
Misc Feature(1)2..2(+)
Misc Feature(2)6..6(+)
Misc Feature(3)7..7(+)
Misc Feature(4)11..11(+)
Misc Feature(5)17..19(+)
Misc Feature(6)497..502(+)
Misc Feature(7)716..1069(+)
Exon (1)1..307
Gene:BDNF
Gene Synonym:
Exon (2)308..3998
Gene:BDNF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001143813
Organism Homo sapiens (human)
Definition Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 13, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001143813

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26594
Clone ID Related Accession (Same CDS sequence) NM_001143805 , NM_001143806 , NM_001143807 , NM_001143808 , NM_001143811 , NM_001143812 , NM_001143813 , NM_001143814 , NM_001143816 , NM_001709 , NM_170732 , NM_170733 , NM_170735
Accession Version NM_001143814.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 744bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product brain-derived neurotrophic factor isoform a preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF689016.1. Transcript Variant: This variant (14), also known as VIb-IXbd, differs in the 5' UTR and represents use of an alternate promoter compared to variant 1. Variants 1, 2, 4, 5, and 7-16 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF689016.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2148874, SAMEA962344 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001137286.1
CDS454..1197
Misc Feature(1)2..2(+)
Misc Feature(2)6..6(+)
Misc Feature(3)7..7(+)
Misc Feature(4)11..11(+)
Misc Feature(5)424..426(+)
Misc Feature(6)622..627(+)
Misc Feature(7)841..1194(+)
Exon (1)1..325
Gene:BDNF
Gene Synonym:
Exon (2)326..432
Gene:BDNF
Gene Synonym:
Exon (3)433..4123
Gene:BDNF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001143814
Organism Homo sapiens (human)
Definition Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 14, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001143814

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26594
Clone ID Related Accession (Same CDS sequence) NM_001143805 , NM_001143806 , NM_001143807 , NM_001143808 , NM_001143811 , NM_001143812 , NM_001143813 , NM_001143814 , NM_001143816 , NM_001709 , NM_170732 , NM_170733 , NM_170735
Accession Version NM_001143816.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 744bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product brain-derived neurotrophic factor isoform a preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF689010.1. Transcript Variant: This variant (16), also known as IXabd, differs in the 5' UTR compared to variant 1. Variants 1, 2, 4, 5, and 7-16 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF689010.1, W48706.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA962344 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001137288.1
CDS852..1595
Misc Feature(1)822..824(+)
Misc Feature(2)1020..1025(+)
Misc Feature(3)1239..1592(+)
Exon (1)1..830
Gene:BDNF
Gene Synonym:
Exon (2)831..4521
Gene:BDNF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001143816
Organism Homo sapiens (human)
Definition Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 16, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001143816

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTTG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CAAACATCCG AGGACAAGGT GGCTTGGCCT ACCCAGGTGT GCGGACCCAT
GGGACTCTGG AGAGCGTGAA TGGGCCCAAG GCAGGTTCAA GAGGCTTGAC ATCATTGGCT
GACACTTTCG AACACGTGAT AGAAGAGCTG TTGGATGAGG ACCAGAAAGT TCGGCCCAAT
GAAGAAAACA ATAAGGACGC AGACTTGTAC ACGTCCAGGG TGATGCTCAG TAGTCAAGTG
CCTTTGGAGC CTCCTCTTCT CTTTCTGCTG GAGGAATACA AAAATTACCT AGATGCTGCA
AACATGTCCA TGAGGGTCCG GCGCCACTCT GACCCTGCCC GCCGAGGGGA GCTGAGCGTG
TGTGACAGTA TTAGTGAGTG GGTAACGGCG GCAGACAAAA AGACTGCAGT GGACATGTCG
GGCGGGACGG TCACAGTCCT TGAAAAGGTC CCTGTATCAA AAGGCCAACT GAAGCAATAC
TTCTACGAGA CCAAGTGCAA TCCCATGGGT TACACAAAAG AAGGCTGCAG GGGCATAGAC
AAAAGGCATT GGAACTCCCA GTGCCGAACT ACCCAGTCGT ACGTGCGGGC CCTTACCATG
GATAGCAAAA AGAGAATTGG CTGGCGATTC ATAAGGATAG ACACTTCTTG TGTATGTACA
TTGACCATTA AAAGGGGAAG ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Dosage effects of BDNF Val66Met polymorphism on cortical surface area and functional connectivity
J. Neurosci. 34 (7), 2645-2651 (2014)
Wang C, Zhang Y, Liu B, Long H, Yu C and Jiang T.


book

An adaptive role for BDNF Val66Met polymorphism in motor recovery in chronic stroke
J. Neurosci. 34 (7), 2493-2502 (2014)
Qin L, Jing D, Parauda S, Carmel J, Ratan RR, Lee FS and Cho S.


book

Impact of BDNF -196 G>A and BDNF -270 C>T polymorphisms on stroke rehabilitation outcome: sex and age differences
Top Stroke Rehabil 21 (SUPPL 1), S33-S41 (2014)
Mirowska-Guzel D, Gromadzka G, Mendel T, Janus-Laszuk B, Dzierka J, Sarzynska-Dlugosz I, Czlonkowski A and Czlonkowska A.


book

[The relationship between BDNF gene polymorphisms and alcoholics in Japan]
Nihon Arukoru Yakubutsu Igakkai Zasshi 48 (6), 407-414 (2013)
Narita,S., Nagahori,K., Yoshihara,E., Nishizawa,D., Ikeda,K., Kawai,A. and Iwahashi,K.


book

[Interaction effects of anxiety and the BDNF Val66Met polymorphism on attention in patients with schizophrenia spectrum disorders]
Zh Nevrol Psikhiatr Im S S Korsakova 113 (10), 49-54 (2013)
Golimbet,V.E., Lebedeva,I.S., Alfimova,M.V., Korovaitseva,G.I. and Lezheiko,T.V.


book

Hirschsprung Disease Overview
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Parisi,M.A.


book

Characterization of the 5'-flanking region of the human brain-derived neurotrophic factor gene
Biochem. Biophys. Res. Commun. 182 (1), 325-332 (1992)
Shintani A, Ono Y, Kaisho Y and Igarashi K.


book

Chromosomal mapping of brain-derived neurotrophic factor and neurotrophin-3 genes in man and mouse
Genomics 10 (3), 569-575 (1991)
Ozcelik T, Rosenthal A and Francke U.


book

Human and rat brain-derived neurotrophic factor and neurotrophin-3: gene structures, distributions, and chromosomal localizations
Genomics 10 (3), 558-568 (1991)
Maisonpierre PC, Le Beau MM, Espinosa R 3rd, Ip NY, Belluscio L, de la Monte SM, Squinto S, Furth ME and Yancopoulos GD.


book

Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in Xenopus ovary
Neuron 6 (5), 845-858 (1991)
Hallbook F, Ibanez CF and Persson H.


 
*
*
*
*