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SCNN1A cDNA ORF clone, Homo sapiens (human)

Gene Symbol SCNN1A
Entrez Gene ID 6337
Full Name sodium channel, non voltage gated 1 alpha subunit
Synonyms BESC2, ENaCa, ENaCalpha, SCNEA, SCNN1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12p13

Summary Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2009]. lac of sum
Disorder MIM:

600228

Disorder Html: Pseudohypoaldosteronism, type I, 264350 (3); Bronchiectasis with

mRNA and Protein(s)

mRNA Protein Name
NM_001038 NP_001029 amiloride-sensitive sodium channel subunit alpha isoform 1
NM_001159575 NP_001153047 amiloride-sensitive sodium channel subunit alpha isoform 3
NM_001159576 NP_001153048 amiloride-sensitive sodium channel subunit alpha isoform 2



Homo sapiens (human) SCNN1A NP_001153047.1
Pan troglodytes (chimpanzee) SCNN1A XP_508948.4
Macaca mulatta (Rhesus monkey) SCNN1A XP_001103017.2
Canis lupus familiaris (dog) SCNN1A XP_005637310.1
Bos taurus (cattle) SCNN1A NP_777023.1
Mus musculus (house mouse) Scnn1a NP_035454.2
Rattus norvegicus (Norway rat) Scnn1a NP_113736.1
Gallus gallus (chicken) SCNN1A NP_990476.2
Xenopus (Silurana) tropicalis (western clawed frog) scnn1a NP_001184124.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SCNN1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SCNN1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19374
NM_001038 Homo sapiens sodium channel, non voltage gated 1 alpha subunit (SCNN1A), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
OHu14485 NM_001159575 Homo sapiens sodium channel, non voltage gated 1 alpha subunit (SCNN1A), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $489.30
$699.00
OHu14548 NM_001159576 Homo sapiens sodium channel, non voltage gated 1 alpha subunit (SCNN1A), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $517.30
$739.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19374
Clone ID Related Accession (Same CDS sequence) NM_001038
Accession Version NM_001038.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2010bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product amiloride-sensitive sodium channel subunit alpha isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB232484.1, X76180.1 and BU680600.1. This sequence is a reference standard in the RefSeqGene project. On Apr 23, 2009 this sequence version replaced gi:47834319. Transcript Variant: This variant (1, also known as alpha-ENaC1) represents the predominant transcript, and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X76180.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962346 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGAGGGGA ACAAGCTGGA GGAGCAGGAC TCTAGCCCTC CACAGTCCAC TCCAGGGCTC 
ATGAAGGGGA ACAAGCGTGA GGAGCAGGGG CTGGGCCCCG AACCTGCGGC GCCCCAGCAG
CCCACGGCGG AGGAGGAGGC CCTGATCGAG TTCCACCGCT CCTACCGAGA GCTCTTCGAG
TTCTTCTGCA ACAACACCAC CATCCACGGC GCCATCCGCC TGGTGTGCTC CCAGCACAAC
CGCATGAAGA CGGCCTTCTG GGCAGTGCTG TGGCTCTGCA CCTTTGGCAT GATGTACTGG
CAATTCGGCC TGCTTTTCGG AGAGTACTTC AGCTACCCCG TCAGCCTCAA CATCAACCTC
AACTCGGACA AGCTCGTCTT CCCCGCAGTG ACCATCTGCA CCCTCAATCC CTACAGGTAC
CCGGAAATTA AAGAGGAGCT GGAGGAGCTG GACCGCATCA CAGAGCAGAC GCTCTTTGAC
CTGTACAAAT ACAGCTCCTT CACCACTCTC GTGGCCGGCT CCCGCAGCCG TCGCGACCTG
CGGGGGACTC TGCCGCACCC CTTGCAGCGC CTGAGGGTCC CGCCCCCGCC TCACGGGGCC
CGTCGAGCCC GTAGCGTGGC CTCCAGCTTG CGGGACAACA ACCCCCAGGT GGACTGGAAG
GACTGGAAGA TCGGCTTCCA GCTGTGCAAC CAGAACAAAT CGGACTGCTT CTACCAGACA
TACTCATCAG GGGTGGATGC GGTGAGGGAG TGGTACCGCT TCCACTACAT CAACATCCTG
TCGAGGCTGC CAGAGACTCT GCCATCCCTG GAGGAGGACA CGCTGGGCAA CTTCATCTTC
GCCTGCCGCT TCAACCAGGT CTCCTGCAAC CAGGCGAATT ACTCTCACTT CCACCACCCG
ATGTATGGAA ACTGCTATAC TTTCAATGAC AAGAACAACT CCAACCTCTG GATGTCTTCC
ATGCCTGGAA TCAACAACGG TCTGTCCCTG ATGCTGCGCG CAGAGCAGAA TGACTTCATT
CCCCTGCTGT CCACAGTGAC TGGGGCCCGG GTAATGGTGC ACGGGCAGGA TGAACCTGCC
TTTATGGATG ATGGTGGCTT TAACTTGCGG CCTGGCGTGG AGACCTCCAT CAGCATGAGG
AAGGAAACCC TGGACAGACT TGGGGGCGAT TATGGCGACT GCACCAAGAA TGGCAGTGAT
GTTCCTGTTG AGAACCTTTA CCCTTCAAAG TACACACAGC AGGTGTGTAT TCACTCCTGC
TTCCAGGAGA GCATGATCAA GGAGTGTGGC TGTGCCTACA TCTTCTATCC GCGGCCCCAG
AACGTGGAGT ACTGTGACTA CAGAAAGCAC AGTTCCTGGG GGTACTGCTA CTATAAGCTC
CAGGTTGACT TCTCCTCAGA CCACCTGGGC TGTTTCACCA AGTGCCGGAA GCCATGCAGC
GTGACCAGCT ACCAGCTCTC TGCTGGTTAC TCACGATGGC CCTCGGTGAC ATCCCAGGAA
TGGGTCTTCC AGATGCTATC GCGACAGAAC AATTACACCG TCAACAACAA GAGAAATGGA
GTGGCCAAAG TCAACATCTT CTTCAAGGAG CTGAACTACA AAACCAATTC TGAGTCTCCC
TCTGTCACGA TGGTCACCCT CCTGTCCAAC CTGGGCAGCC AGTGGAGCCT GTGGTTCGGC
TCCTCGGTGT TGTCTGTGGT GGAGATGGCT GAGCTCGTCT TTGACCTGCT GGTCATCATG
TTCCTCATGC TGCTCCGAAG GTTCCGAAGC CGATACTGGT CTCCAGGCCG AGGGGGCAGG
GGTGCTCAGG AGGTAGCCTC CACCCTGGCA TCCTCCCCTC CTTCCCACTT CTGCCCCCAC
CCCATGTCTC TGTCCTTGTC CCAGCCAGGC CCTGCTCCCT CTCCAGCCTT GACAGCCCCT
CCCCCTGCCT ATGCCACCCT GGGCCCCCGC CCATCTCCAG GGGGCTCTGC AGGGGCCAGT
TCCTCCACCT GTCCTCTGGG GGGGCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001029.1
CDS290..2299
Misc Feature(1)164..166(+)
Misc Feature(2)449..2230(+)
Misc Feature(3)473..2005(+)
Misc Feature(4)548..619(+)
Misc Feature(5)1919..2011(+)
Exon (1)1..235
Gene:SCNN1A
Gene Synonym:
Exon (2)236..705
Gene:SCNN1A
Gene Synonym:
Exon (3)706..973
Gene:SCNN1A
Gene Synonym:
Exon (4)974..1164
Gene:SCNN1A
Gene Synonym:
Exon (5)1165..1268
Gene:SCNN1A
Gene Synonym:
Exon (6)1269..1432
Gene:SCNN1A
Gene Synonym:
Exon (7)1433..1531
Gene:SCNN1A
Gene Synonym:
Exon (8)1532..1649
Gene:SCNN1A
Gene Synonym:
Exon (9)1650..1728
Gene:SCNN1A
Gene Synonym:
Exon (10)1729..1786
Gene:SCNN1A
Gene Synonym:
Exon (11)1787..1842
Gene:SCNN1A
Gene Synonym:
Exon (12)1843..1918
Gene:SCNN1A
Gene Synonym:
Exon (13)1919..3329
Gene:SCNN1A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001038
Organism Homo sapiens (human)
Definition Homo sapiens sodium channel, non voltage gated 1 alpha subunit (SCNN1A), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001038

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGAGGGGA ACAAGCTGGA GGAGCAGGAC TCTAGCCCTC CACAGTCCAC TCCAGGGCTC 
ATGAAGGGGA ACAAGCGTGA GGAGCAGGGG CTGGGCCCCG AACCTGCGGC GCCCCAGCAG
CCCACGGCGG AGGAGGAGGC CCTGATCGAG TTCCACCGCT CCTACCGAGA GCTCTTCGAG
TTCTTCTGCA ACAACACCAC CATCCACGGC GCCATCCGCC TGGTGTGCTC CCAGCACAAC
CGCATGAAGA CGGCCTTCTG GGCAGTGCTG TGGCTCTGCA CCTTTGGCAT GATGTACTGG
CAATTCGGCC TGCTTTTCGG AGAGTACTTC AGCTACCCCG TCAGCCTCAA CATCAACCTC
AACTCGGACA AGCTCGTCTT CCCCGCAGTG ACCATCTGCA CCCTCAATCC CTACAGGTAC
CCGGAAATTA AAGAGGAGCT GGAGGAGCTG GACCGCATCA CAGAGCAGAC GCTCTTTGAC
CTGTACAAAT ACAGCTCCTT CACCACTCTC GTGGCCGGCT CCCGCAGCCG TCGCGACCTG
CGGGGGACTC TGCCGCACCC CTTGCAGCGC CTGAGGGTCC CGCCCCCGCC TCACGGGGCC
CGTCGAGCCC GTAGCGTGGC CTCCAGCTTG CGGGACAACA ACCCCCAGGT GGACTGGAAG
GACTGGAAGA TCGGCTTCCA GCTGTGCAAC CAGAACAAAT CGGACTGCTT CTACCAGACA
TACTCATCAG GGGTGGATGC GGTGAGGGAG TGGTACCGCT TCCACTACAT CAACATCCTG
TCGAGGCTGC CAGAGACTCT GCCATCCCTG GAGGAGGACA CGCTGGGCAA CTTCATCTTC
GCCTGCCGCT TCAACCAGGT CTCCTGCAAC CAGGCGAATT ACTCTCACTT CCACCACCCG
ATGTATGGAA ACTGCTATAC TTTCAATGAC AAGAACAACT CCAACCTCTG GATGTCTTCC
ATGCCTGGAA TCAACAACGG TCTGTCCCTG ATGCTGCGCG CAGAGCAGAA TGACTTCATT
CCCCTGCTGT CCACAGTGAC TGGGGCCCGG GTAATGGTGC ACGGGCAGGA TGAACCTGCC
TTTATGGATG ATGGTGGCTT TAACTTGCGG CCTGGCGTGG AGACCTCCAT CAGCATGAGG
AAGGAAACCC TGGACAGACT TGGGGGCGAT TATGGCGACT GCACCAAGAA TGGCAGTGAT
GTTCCTGTTG AGAACCTTTA CCCTTCAAAG TACACACAGC AGGTGTGTAT TCACTCCTGC
TTCCAGGAGA GCATGATCAA GGAGTGTGGC TGTGCCTACA TCTTCTATCC GCGGCCCCAG
AACGTGGAGT ACTGTGACTA CAGAAAGCAC AGTTCCTGGG GGTACTGCTA CTATAAGCTC
CAGGTTGACT TCTCCTCAGA CCACCTGGGC TGTTTCACCA AGTGCCGGAA GCCATGCAGC
GTGACCAGCT ACCAGCTCTC TGCTGGTTAC TCACGATGGC CCTCGGTGAC ATCCCAGGAA
TGGGTCTTCC AGATGCTATC GCGACAGAAC AATTACACCG TCAACAACAA GAGAAATGGA
GTGGCCAAAG TCAACATCTT CTTCAAGGAG CTGAACTACA AAACCAATTC TGAGTCTCCC
TCTGTCACGA TGGTCACCCT CCTGTCCAAC CTGGGCAGCC AGTGGAGCCT GTGGTTCGGC
TCCTCGGTGT TGTCTGTGGT GGAGATGGCT GAGCTCGTCT TTGACCTGCT GGTCATCATG
TTCCTCATGC TGCTCCGAAG GTTCCGAAGC CGATACTGGT CTCCAGGCCG AGGGGGCAGG
GGTGCTCAGG AGGTAGCCTC CACCCTGGCA TCCTCCCCTC CTTCCCACTT CTGCCCCCAC
CCCATGTCTC TGTCCTTGTC CCAGCCAGGC CCTGCTCCCT CTCCAGCCTT GACAGCCCCT
CCCCCTGCCT ATGCCACCCT GGGCCCCCGC CCATCTCCAG GGGGCTCTGC AGGGGCCAGT
TCCTCCACCT GTCCTCTGGG GGGGCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14485
Clone ID Related Accession (Same CDS sequence) NM_001159575
Accession Version NM_001159575.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2079bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product amiloride-sensitive sodium channel subunit alpha isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK304379.1, X76180.1 and BU680600.1. Transcript Variant: This variant (3) contains an alternate exon in the 5' coding region, and uses an in-frame upstream start codon compared to variant 1. This results in an isoform (3) with a longer N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304379.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGAGCAGTA TCAAGGGGAA CAAGCTGGAG GAGCAGGACC CTAGACCTCT GCAGCCCATA 
CCAGGTCTCA TGGAGGGGAA CAAGCTGGAG GAGCAGGACT CTAGCCCTCC ACAGTCCACT
CCAGGGCTCA TGAAGGGGAA CAAGCGTGAG GAGCAGGGGC TGGGCCCCGA ACCTGCGGCG
CCCCAGCAGC CCACGGCGGA GGAGGAGGCC CTGATCGAGT TCCACCGCTC CTACCGAGAG
CTCTTCGAGT TCTTCTGCAA CAACACCACC ATCCACGGCG CCATCCGCCT GGTGTGCTCC
CAGCACAACC GCATGAAGAC GGCCTTCTGG GCAGTGCTGT GGCTCTGCAC CTTTGGCATG
ATGTACTGGC AATTCGGCCT GCTTTTCGGA GAGTACTTCA GCTACCCCGT CAGCCTCAAC
ATCAACCTCA ACTCGGACAA GCTCGTCTTC CCCGCAGTGA CCATCTGCAC CCTCAATCCC
TACAGGTACC CGGAAATTAA AGAGGAGCTG GAGGAGCTGG ACCGCATCAC AGAGCAGACG
CTCTTTGACC TGTACAAATA CAGCTCCTTC ACCACTCTCG TGGCCGGCTC CCGCAGCCGT
CGCGACCTGC GGGGGACTCT GCCGCACCCC TTGCAGCGCC TGAGGGTCCC GCCCCCGCCT
CACGGGGCCC GTCGAGCCCG TAGCGTGGCC TCCAGCTTGC GGGACAACAA CCCCCAGGTG
GACTGGAAGG ACTGGAAGAT CGGCTTCCAG CTGTGCAACC AGAACAAATC GGACTGCTTC
TACCAGACAT ACTCATCAGG GGTGGATGCG GTGAGGGAGT GGTACCGCTT CCACTACATC
AACATCCTGT CGAGGCTGCC AGAGACTCTG CCATCCCTGG AGGAGGACAC GCTGGGCAAC
TTCATCTTCG CCTGCCGCTT CAACCAGGTC TCCTGCAACC AGGCGAATTA CTCTCACTTC
CACCACCCGA TGTATGGAAA CTGCTATACT TTCAATGACA AGAACAACTC CAACCTCTGG
ATGTCTTCCA TGCCTGGAAT CAACAACGGT CTGTCCCTGA TGCTGCGCGC AGAGCAGAAT
GACTTCATTC CCCTGCTGTC CACAGTGACT GGGGCCCGGG TAATGGTGCA CGGGCAGGAT
GAACCTGCCT TTATGGATGA TGGTGGCTTT AACTTGCGGC CTGGCGTGGA GACCTCCATC
AGCATGAGGA AGGAAACCCT GGACAGACTT GGGGGCGATT ATGGCGACTG CACCAAGAAT
GGCAGTGATG TTCCTGTTGA GAACCTTTAC CCTTCAAAGT ACACACAGCA GGTGTGTATT
CACTCCTGCT TCCAGGAGAG CATGATCAAG GAGTGTGGCT GTGCCTACAT CTTCTATCCG
CGGCCCCAGA ACGTGGAGTA CTGTGACTAC AGAAAGCACA GTTCCTGGGG GTACTGCTAC
TATAAGCTCC AGGTTGACTT CTCCTCAGAC CACCTGGGCT GTTTCACCAA GTGCCGGAAG
CCATGCAGCG TGACCAGCTA CCAGCTCTCT GCTGGTTACT CACGATGGCC CTCGGTGACA
TCCCAGGAAT GGGTCTTCCA GATGCTATCG CGACAGAACA ATTACACCGT CAACAACAAG
AGAAATGGAG TGGCCAAAGT CAACATCTTC TTCAAGGAGC TGAACTACAA AACCAATTCT
GAGTCTCCCT CTGTCACGAT GGTCACCCTC CTGTCCAACC TGGGCAGCCA GTGGAGCCTG
TGGTTCGGCT CCTCGGTGTT GTCTGTGGTG GAGATGGCTG AGCTCGTCTT TGACCTGCTG
GTCATCATGT TCCTCATGCT GCTCCGAAGG TTCCGAAGCC GATACTGGTC TCCAGGCCGA
GGGGGCAGGG GTGCTCAGGA GGTAGCCTCC ACCCTGGCAT CCTCCCCTCC TTCCCACTTC
TGCCCCCACC CCATGTCTCT GTCCTTGTCC CAGCCAGGCC CTGCTCCCTC TCCAGCCTTG
ACAGCCCCTC CCCCTGCCTA TGCCACCCTG GGCCCCCGCC CATCTCCAGG GGGCTCTGCA
GGGGCCAGTT CCTCCACCTG TCCTCTGGGG GGGCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001153047.1
CDS92..2170
Misc Feature(1)53..55(+)
Misc Feature(2)320..2101(+)
Misc Feature(3)344..1876(+)
Exon (1)1..106
Gene:SCNN1A
Gene Synonym:
Exon (2)107..576
Gene:SCNN1A
Gene Synonym:
Exon (3)577..844
Gene:SCNN1A
Gene Synonym:
Exon (4)845..1035
Gene:SCNN1A
Gene Synonym:
Exon (5)1036..1139
Gene:SCNN1A
Gene Synonym:
Exon (6)1140..1303
Gene:SCNN1A
Gene Synonym:
Exon (7)1304..1402
Gene:SCNN1A
Gene Synonym:
Exon (8)1403..1520
Gene:SCNN1A
Gene Synonym:
Exon (9)1521..1599
Gene:SCNN1A
Gene Synonym:
Exon (10)1600..1657
Gene:SCNN1A
Gene Synonym:
Exon (11)1658..1713
Gene:SCNN1A
Gene Synonym:
Exon (12)1714..1789
Gene:SCNN1A
Gene Synonym:
Exon (13)1790..3200
Gene:SCNN1A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001159575
Organism Homo sapiens (human)
Definition Homo sapiens sodium channel, non voltage gated 1 alpha subunit (SCNN1A), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001159575

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGAGCAGTA TCAAGGGGAA CAAGCTGGAG GAGCAGGACC CTAGACCTCT GCAGCCCATA 
CCAGGTCTCA TGGAGGGGAA CAAGCTGGAG GAGCAGGACT CTAGCCCTCC ACAGTCCACT
CCAGGGCTCA TGAAGGGGAA CAAGCGTGAG GAGCAGGGGC TGGGCCCCGA ACCTGCGGCG
CCCCAGCAGC CCACGGCGGA GGAGGAGGCC CTGATCGAGT TCCACCGCTC CTACCGAGAG
CTCTTCGAGT TCTTCTGCAA CAACACCACC ATCCACGGCG CCATCCGCCT GGTGTGCTCC
CAGCACAACC GCATGAAGAC GGCCTTCTGG GCAGTGCTGT GGCTCTGCAC CTTTGGCATG
ATGTACTGGC AATTCGGCCT GCTTTTCGGA GAGTACTTCA GCTACCCCGT CAGCCTCAAC
ATCAACCTCA ACTCGGACAA GCTCGTCTTC CCCGCAGTGA CCATCTGCAC CCTCAATCCC
TACAGGTACC CGGAAATTAA AGAGGAGCTG GAGGAGCTGG ACCGCATCAC AGAGCAGACG
CTCTTTGACC TGTACAAATA CAGCTCCTTC ACCACTCTCG TGGCCGGCTC CCGCAGCCGT
CGCGACCTGC GGGGGACTCT GCCGCACCCC TTGCAGCGCC TGAGGGTCCC GCCCCCGCCT
CACGGGGCCC GTCGAGCCCG TAGCGTGGCC TCCAGCTTGC GGGACAACAA CCCCCAGGTG
GACTGGAAGG ACTGGAAGAT CGGCTTCCAG CTGTGCAACC AGAACAAATC GGACTGCTTC
TACCAGACAT ACTCATCAGG GGTGGATGCG GTGAGGGAGT GGTACCGCTT CCACTACATC
AACATCCTGT CGAGGCTGCC AGAGACTCTG CCATCCCTGG AGGAGGACAC GCTGGGCAAC
TTCATCTTCG CCTGCCGCTT CAACCAGGTC TCCTGCAACC AGGCGAATTA CTCTCACTTC
CACCACCCGA TGTATGGAAA CTGCTATACT TTCAATGACA AGAACAACTC CAACCTCTGG
ATGTCTTCCA TGCCTGGAAT CAACAACGGT CTGTCCCTGA TGCTGCGCGC AGAGCAGAAT
GACTTCATTC CCCTGCTGTC CACAGTGACT GGGGCCCGGG TAATGGTGCA CGGGCAGGAT
GAACCTGCCT TTATGGATGA TGGTGGCTTT AACTTGCGGC CTGGCGTGGA GACCTCCATC
AGCATGAGGA AGGAAACCCT GGACAGACTT GGGGGCGATT ATGGCGACTG CACCAAGAAT
GGCAGTGATG TTCCTGTTGA GAACCTTTAC CCTTCAAAGT ACACACAGCA GGTGTGTATT
CACTCCTGCT TCCAGGAGAG CATGATCAAG GAGTGTGGCT GTGCCTACAT CTTCTATCCG
CGGCCCCAGA ACGTGGAGTA CTGTGACTAC AGAAAGCACA GTTCCTGGGG GTACTGCTAC
TATAAGCTCC AGGTTGACTT CTCCTCAGAC CACCTGGGCT GTTTCACCAA GTGCCGGAAG
CCATGCAGCG TGACCAGCTA CCAGCTCTCT GCTGGTTACT CACGATGGCC CTCGGTGACA
TCCCAGGAAT GGGTCTTCCA GATGCTATCG CGACAGAACA ATTACACCGT CAACAACAAG
AGAAATGGAG TGGCCAAAGT CAACATCTTC TTCAAGGAGC TGAACTACAA AACCAATTCT
GAGTCTCCCT CTGTCACGAT GGTCACCCTC CTGTCCAACC TGGGCAGCCA GTGGAGCCTG
TGGTTCGGCT CCTCGGTGTT GTCTGTGGTG GAGATGGCTG AGCTCGTCTT TGACCTGCTG
GTCATCATGT TCCTCATGCT GCTCCGAAGG TTCCGAAGCC GATACTGGTC TCCAGGCCGA
GGGGGCAGGG GTGCTCAGGA GGTAGCCTCC ACCCTGGCAT CCTCCCCTCC TTCCCACTTC
TGCCCCCACC CCATGTCTCT GTCCTTGTCC CAGCCAGGCC CTGCTCCCTC TCCAGCCTTG
ACAGCCCCTC CCCCTGCCTA TGCCACCCTG GGCCCCCGCC CATCTCCAGG GGGCTCTGCA
GGGGCCAGTT CCTCCACCTG TCCTCTGGGG GGGCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14548
Clone ID Related Accession (Same CDS sequence) NM_001159576
Accession Version NM_001159576.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2187bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product amiloride-sensitive sodium channel subunit alpha isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK172792.1, BC006526.2 and BU680600.1. Transcript Variant: This variant (2, also known as alpha-ENaC2) contains an alternate segment in the 5' coding region, and uses an in-frame upstream start codon compared to variant 1. This results in an isoform (2) with a longer N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK172792.1, BC006526.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962346 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGGCATGG CCAGGGGCAG CCTCACTCGG GTTCCAGGGG TGATGGGAGA GGGCACTCAG 
GGCCCAGAGC TCAGCCTTGA CCCTGACCCT TGCTCTCCCC AATCCACTCC GGGGCTCATG
AAGGGGAACA AGCTGGAGGA GCAGGACCCT AGACCTCTGC AGCCCATACC AGGTCTCATG
GAGGGGAACA AGCTGGAGGA GCAGGACTCT AGCCCTCCAC AGTCCACTCC AGGGCTCATG
AAGGGGAACA AGCGTGAGGA GCAGGGGCTG GGCCCCGAAC CTGCGGCGCC CCAGCAGCCC
ACGGCGGAGG AGGAGGCCCT GATCGAGTTC CACCGCTCCT ACCGAGAGCT CTTCGAGTTC
TTCTGCAACA ACACCACCAT CCACGGCGCC ATCCGCCTGG TGTGCTCCCA GCACAACCGC
ATGAAGACGG CCTTCTGGGC AGTGCTGTGG CTCTGCACCT TTGGCATGAT GTACTGGCAA
TTCGGCCTGC TTTTCGGAGA GTACTTCAGC TACCCCGTCA GCCTCAACAT CAACCTCAAC
TCGGACAAGC TCGTCTTCCC CGCAGTGACC ATCTGCACCC TCAATCCCTA CAGGTACCCG
GAAATTAAAG AGGAGCTGGA GGAGCTGGAC CGCATCACAG AGCAGACGCT CTTTGACCTG
TACAAATACA GCTCCTTCAC CACTCTCGTG GCCGGCTCCC GCAGCCGTCG CGACCTGCGG
GGGACTCTGC CGCACCCCTT GCAGCGCCTG AGGGTCCCGC CCCCGCCTCA CGGGGCCCGT
CGAGCCCGTA GCGTGGCCTC CAGCTTGCGG GACAACAACC CCCAGGTGGA CTGGAAGGAC
TGGAAGATCG GCTTCCAGCT GTGCAACCAG AACAAATCGG ACTGCTTCTA CCAGACATAC
TCATCAGGGG TGGATGCGGT GAGGGAGTGG TACCGCTTCC ACTACATCAA CATCCTGTCG
AGGCTGCCAG AGACTCTGCC ATCCCTGGAG GAGGACACGC TGGGCAACTT CATCTTCGCC
TGCCGCTTCA ACCAGGTCTC CTGCAACCAG GCGAATTACT CTCACTTCCA CCACCCGATG
TATGGAAACT GCTATACTTT CAATGACAAG AACAACTCCA ACCTCTGGAT GTCTTCCATG
CCTGGAATCA ACAACGGTCT GTCCCTGATG CTGCGCGCAG AGCAGAATGA CTTCATTCCC
CTGCTGTCCA CAGTGACTGG GGCCCGGGTA ATGGTGCACG GGCAGGATGA ACCTGCCTTT
ATGGATGATG GTGGCTTTAA CTTGCGGCCT GGCGTGGAGA CCTCCATCAG CATGAGGAAG
GAAACCCTGG ACAGACTTGG GGGCGATTAT GGCGACTGCA CCAAGAATGG CAGTGATGTT
CCTGTTGAGA ACCTTTACCC TTCAAAGTAC ACACAGCAGG TGTGTATTCA CTCCTGCTTC
CAGGAGAGCA TGATCAAGGA GTGTGGCTGT GCCTACATCT TCTATCCGCG GCCCCAGAAC
GTGGAGTACT GTGACTACAG AAAGCACAGT TCCTGGGGGT ACTGCTACTA TAAGCTCCAG
GTTGACTTCT CCTCAGACCA CCTGGGCTGT TTCACCAAGT GCCGGAAGCC ATGCAGCGTG
ACCAGCTACC AGCTCTCTGC TGGTTACTCA CGATGGCCCT CGGTGACATC CCAGGAATGG
GTCTTCCAGA TGCTATCGCG ACAGAACAAT TACACCGTCA ACAACAAGAG AAATGGAGTG
GCCAAAGTCA ACATCTTCTT CAAGGAGCTG AACTACAAAA CCAATTCTGA GTCTCCCTCT
GTCACGATGG TCACCCTCCT GTCCAACCTG GGCAGCCAGT GGAGCCTGTG GTTCGGCTCC
TCGGTGTTGT CTGTGGTGGA GATGGCTGAG CTCGTCTTTG ACCTGCTGGT CATCATGTTC
CTCATGCTGC TCCGAAGGTT CCGAAGCCGA TACTGGTCTC CAGGCCGAGG GGGCAGGGGT
GCTCAGGAGG TAGCCTCCAC CCTGGCATCC TCCCCTCCTT CCCACTTCTG CCCCCACCCC
ATGTCTCTGT CCTTGTCCCA GCCAGGCCCT GCTCCCTCTC CAGCCTTGAC AGCCCCTCCC
CCTGCCTATG CCACCCTGGG CCCCCGCCCA TCTCCAGGGG GCTCTGCAGG GGCCAGTTCC
TCCACCTGTC CTCTGGGGGG GCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001153048.1
CDS265..2451
Misc Feature(1)40..42(+)
Misc Feature(2)601..2382(+)
Misc Feature(3)625..2157(+)
Exon (1)1..857
Gene:SCNN1A
Gene Synonym:
Exon (2)858..1125
Gene:SCNN1A
Gene Synonym:
Exon (3)1126..1316
Gene:SCNN1A
Gene Synonym:
Exon (4)1317..1420
Gene:SCNN1A
Gene Synonym:
Exon (5)1421..1584
Gene:SCNN1A
Gene Synonym:
Exon (6)1585..1683
Gene:SCNN1A
Gene Synonym:
Exon (7)1684..1801
Gene:SCNN1A
Gene Synonym:
Exon (8)1802..1880
Gene:SCNN1A
Gene Synonym:
Exon (9)1881..1938
Gene:SCNN1A
Gene Synonym:
Exon (10)1939..1994
Gene:SCNN1A
Gene Synonym:
Exon (11)1995..2070
Gene:SCNN1A
Gene Synonym:
Exon (12)2071..3481
Gene:SCNN1A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001159576
Organism Homo sapiens (human)
Definition Homo sapiens sodium channel, non voltage gated 1 alpha subunit (SCNN1A), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001159576

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGGCATGG CCAGGGGCAG CCTCACTCGG GTTCCAGGGG TGATGGGAGA GGGCACTCAG 
GGCCCAGAGC TCAGCCTTGA CCCTGACCCT TGCTCTCCCC AATCCACTCC GGGGCTCATG
AAGGGGAACA AGCTGGAGGA GCAGGACCCT AGACCTCTGC AGCCCATACC AGGTCTCATG
GAGGGGAACA AGCTGGAGGA GCAGGACTCT AGCCCTCCAC AGTCCACTCC AGGGCTCATG
AAGGGGAACA AGCGTGAGGA GCAGGGGCTG GGCCCCGAAC CTGCGGCGCC CCAGCAGCCC
ACGGCGGAGG AGGAGGCCCT GATCGAGTTC CACCGCTCCT ACCGAGAGCT CTTCGAGTTC
TTCTGCAACA ACACCACCAT CCACGGCGCC ATCCGCCTGG TGTGCTCCCA GCACAACCGC
ATGAAGACGG CCTTCTGGGC AGTGCTGTGG CTCTGCACCT TTGGCATGAT GTACTGGCAA
TTCGGCCTGC TTTTCGGAGA GTACTTCAGC TACCCCGTCA GCCTCAACAT CAACCTCAAC
TCGGACAAGC TCGTCTTCCC CGCAGTGACC ATCTGCACCC TCAATCCCTA CAGGTACCCG
GAAATTAAAG AGGAGCTGGA GGAGCTGGAC CGCATCACAG AGCAGACGCT CTTTGACCTG
TACAAATACA GCTCCTTCAC CACTCTCGTG GCCGGCTCCC GCAGCCGTCG CGACCTGCGG
GGGACTCTGC CGCACCCCTT GCAGCGCCTG AGGGTCCCGC CCCCGCCTCA CGGGGCCCGT
CGAGCCCGTA GCGTGGCCTC CAGCTTGCGG GACAACAACC CCCAGGTGGA CTGGAAGGAC
TGGAAGATCG GCTTCCAGCT GTGCAACCAG AACAAATCGG ACTGCTTCTA CCAGACATAC
TCATCAGGGG TGGATGCGGT GAGGGAGTGG TACCGCTTCC ACTACATCAA CATCCTGTCG
AGGCTGCCAG AGACTCTGCC ATCCCTGGAG GAGGACACGC TGGGCAACTT CATCTTCGCC
TGCCGCTTCA ACCAGGTCTC CTGCAACCAG GCGAATTACT CTCACTTCCA CCACCCGATG
TATGGAAACT GCTATACTTT CAATGACAAG AACAACTCCA ACCTCTGGAT GTCTTCCATG
CCTGGAATCA ACAACGGTCT GTCCCTGATG CTGCGCGCAG AGCAGAATGA CTTCATTCCC
CTGCTGTCCA CAGTGACTGG GGCCCGGGTA ATGGTGCACG GGCAGGATGA ACCTGCCTTT
ATGGATGATG GTGGCTTTAA CTTGCGGCCT GGCGTGGAGA CCTCCATCAG CATGAGGAAG
GAAACCCTGG ACAGACTTGG GGGCGATTAT GGCGACTGCA CCAAGAATGG CAGTGATGTT
CCTGTTGAGA ACCTTTACCC TTCAAAGTAC ACACAGCAGG TGTGTATTCA CTCCTGCTTC
CAGGAGAGCA TGATCAAGGA GTGTGGCTGT GCCTACATCT TCTATCCGCG GCCCCAGAAC
GTGGAGTACT GTGACTACAG AAAGCACAGT TCCTGGGGGT ACTGCTACTA TAAGCTCCAG
GTTGACTTCT CCTCAGACCA CCTGGGCTGT TTCACCAAGT GCCGGAAGCC ATGCAGCGTG
ACCAGCTACC AGCTCTCTGC TGGTTACTCA CGATGGCCCT CGGTGACATC CCAGGAATGG
GTCTTCCAGA TGCTATCGCG ACAGAACAAT TACACCGTCA ACAACAAGAG AAATGGAGTG
GCCAAAGTCA ACATCTTCTT CAAGGAGCTG AACTACAAAA CCAATTCTGA GTCTCCCTCT
GTCACGATGG TCACCCTCCT GTCCAACCTG GGCAGCCAGT GGAGCCTGTG GTTCGGCTCC
TCGGTGTTGT CTGTGGTGGA GATGGCTGAG CTCGTCTTTG ACCTGCTGGT CATCATGTTC
CTCATGCTGC TCCGAAGGTT CCGAAGCCGA TACTGGTCTC CAGGCCGAGG GGGCAGGGGT
GCTCAGGAGG TAGCCTCCAC CCTGGCATCC TCCCCTCCTT CCCACTTCTG CCCCCACCCC
ATGTCTCTGT CCTTGTCCCA GCCAGGCCCT GCTCCCTCTC CAGCCTTGAC AGCCCCTCCC
CCTGCCTATG CCACCCTGGG CCCCCGCCCA TCTCCAGGGG GCTCTGCAGG GGCCAGTTCC
TCCACCTGTC CTCTGGGGGG GCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

miR-125b inhibits hepatitis B virus expression in vitro through targeting of the SCNN1A gene
Arch. Virol. 159 (12), 3335-3343 (2014)
Zhang Z, Chen J, He Y, Zhan X, Zhao R, Huang Y, Xu H, Zhu Z and Liu Q.


book

Gene regulation by glucocorticoid in ENaC-mediated Na(+) transport by middle ear epithelial cells
Laryngoscope 124 (2), E27-E33 (2014)
Kim BG, Kim JY, Kim M, Kim CH, Choi JY and Kim SH.


book

Identification of the SPLUNC1 ENaC-inhibitory domain yields novel strategies to treat sodium hyperabsorption in cystic fibrosis airway epithelial cultures
Am. J. Physiol. Lung Cell Mol. Physiol. 305 (12), L990-L1001 (2013)
Hobbs CA, Blanchard MG, Alijevic O, Tan CD, Kellenberger S, Bencharit S, Cao R, Kesimer M, Walton WG, Henderson AG, Redinbo MR, Stutts MJ and Tarran R.


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Molecular basis for pH-dependent mucosal dehydration in cystic fibrosis airways
Proc. Natl. Acad. Sci. U.S.A. 110 (40), 15973-15978 (2013)
Garland AL, Walton WG, Coakley RD, Tan CD, Gilmore RC, Hobbs CA, Tripathy A, Clunes LA, Bencharit S, Stutts MJ, Betts L, Redinbo MR and Tarran R.


book

Novel mutations in the SCNN1A gene causing Pseudohypoaldosteronism type 1
PLoS ONE 8 (6), E65676 (2013)
Wang J, Yu T, Yin L, Li J, Yu L, Shen Y, Yu Y, Shen Y and Fu Q.


book

Mutations in the amiloride-sensitive epithelial sodium channel in patients with cystic fibrosis-like disease
Hum. Mutat. 30 (7), 1093-1103 (2009)
Azad AK, Rauh R, Vermeulen F, Jaspers M, Korbmacher J, Boissier B, Bassinet L, Fichou Y, des Georges M, Stanke F, De Boeck K, Dupont L, Balascakova M, Hjelte L, Lebecque P, Radojkovic D, Castellani C, Schwartz M, Stuhrmann M, Schwarz M, Skalicka V, de Monestrol I, Girodon E, Ferec C, Claustres M, Tummler B, Cassiman JJ, Korbmacher C and Cuppens H.


book

Mutations in subunits of the epithelial sodium channel cause salt wasting with hyperkalaemic acidosis, pseudohypoaldosteronism type 1
Nat. Genet. 12 (3), 248-253 (1996)
Chang SS, Grunder S, Hanukoglu A, Rosler A, Mathew PM, Hanukoglu I, Schild L, Lu Y, Shimkets RA, Nelson-Williams C, Rossier BC and Lifton RP.


book

SCNN1, an epithelial cell sodium channel gene in the conserved linkage group on mouse chromosome 6 and human chromosome 12
Genomics 24 (1), 185-186 (1994)
Meisler MH, Barrow LL, Canessa CM and Rossier BC.


book

Cloning, expression, and tissue distribution of a human amiloride-sensitive Na+ channel
Am. J. Physiol. 266 (6 PT 1), L728-L734 (1994)
McDonald FJ, Snyder PM, McCray PB Jr and Welsh MJ.


book

The lung amiloride-sensitive Na+ channel: biophysical properties, pharmacology, ontogenesis, and molecular cloning
Proc. Natl. Acad. Sci. U.S.A. 91 (1), 247-251 (1994)
Voilley N, Lingueglia E, Champigny G, Mattei MG, Waldmann R, Lazdunski M and Barbry P.


 
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