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CCL2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CCL2
Entrez Gene ID 6347
Full Name chemokine (C-C motif) ligand 2
Synonyms GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, SCYA2, SMC-CF
General protein information
Preferred Names
C-C motif chemokine 2
Names
C-C motif chemokine 2
small-inducible cytokine A2
monocyte secretory protein JE
monocyte chemotactic protein 1
monocyte chemoattractant protein 1
monocyte chemoattractant protein-1
monocyte chemotactic and activating factor
small inducible cytokine subfamily A (Cys-Cys), member 2
small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q11.2-q12

Summary This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]. lac of sum
Disorder MIM:

158105

Disorder Html: {HIV-1, resistance to}, 609423 (3); {Spina bifida, susceptibility

mRNA and Protein(s)

mRNA Protein Name
NM_002982 NP_002973 C-C motif chemokine 2 precursor

KEGG
hsa04060 Cytokine-cytokine receptor interaction
hsa04621 NOD-like receptor signaling pathway
hsa04062 Chemokine signaling pathway
hsa05142 Chagas disease (American trypanosomiasis)
hsa05144 Malaria
hsa05323 Rheumatoid arthritis
hsa05164 Influenza A
hsa05168 Herpes simplex infection
hsa04668 TNF signaling pathway
Reactome
R-HSA-381042 PERK regulates gene expression
R-HSA-380994 ATF4 activates genes
R-HSA-381119 Unfolded Protein Response (UPR)
R-HSA-162582 Signal Transduction
R-HSA-500792 GPCR ligand binding
R-HSA-372790 Signaling by GPCR
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-380108 Chemokine receptors bind chemokines
R-HSA-375276 Peptide ligand-binding receptors
R-HSA-392499 Metabolism of proteins
Pathway Interaction Database
fra_pathway Validated transcriptional targets of AP1 family members Fra1 and Fra2
il23pathway IL23-mediated signaling events
WikiPathways
WP15 Selenium Pathway
WP2374 Oncostatin M Signaling Pathway
WP2036 TWEAK Signaling Pathway
WP2431 Spinal Cord Injury


Homo sapiens (human) CCL2 NP_002973.1
Pan troglodytes (chimpanzee) CCL2 XP_001174551.1
Macaca mulatta (Rhesus monkey) CCL2 NP_001027993.1
Canis lupus familiaris (dog) CCL2 NP_001003297.1
Bos taurus (cattle) CCL2 NP_776431.1
Mus musculus (house mouse) Ccl12 NP_035461.2
Rattus norvegicus (Norway rat) Ccl12 NP_001099292.1

Component

ID Name Evidence
GO:0005576 extracellular region EXP
GO:0005615 extracellular space IEA
GO:0005737 cytoplasm IEA
GO:0043025 neuronal cell body IEA

Function

ID Name Evidence
GO:0004672 protein kinase activity TAS
GO:0004871 signal transducer activity TAS
GO:0005102 receptor binding TAS
GO:0005515 protein binding IPI
GO:0008009 chemokine activity IEA
GO:0008201 heparin binding IEA
GO:0031727 CCR2 chemokine receptor binding ISS
GO:0031727 CCR2 chemokine receptor binding TAS

Process

ID Name Evidence
GO:0001525 angiogenesis TAS
GO:0001666 response to hypoxia IEA
GO:0001938 positive regulation of endothelial cell proliferation IEA
GO:0002548 monocyte chemotaxis IDA
GO:0006468 protein phosphorylation TAS
GO:0006874 cellular calcium ion homeostasis IEA
GO:0006916 anti-apoptosis IDA
GO:0006935 chemotaxis IEA
GO:0006954 inflammatory response IEA
GO:0006959 humoral immune response TAS
GO:0007155 cell adhesion TAS
GO:0007165 signal transduction NAS
GO:0007166 cell surface receptor linked signaling pathway TAS
GO:0007179 transforming growth factor beta receptor signaling pathway IEA
GO:0007186 G-protein coupled receptor protein signaling pathway TAS
GO:0007187 G-protein signaling, coupled to cyclic nucleotide second messenger TAS
GO:0007259 JAK-STAT cascade TAS
GO:0009408 response to heat IEA
GO:0009612 response to mechanical stimulus IEA
GO:0009617 response to bacterium IEP
GO:0009887 organ morphogenesis TAS
GO:0010332 response to gamma radiation IEA
GO:0014823 response to activity IEA
GO:0019079 viral genome replication TAS
GO:0019221 cytokine-mediated signaling pathway IDA
GO:0019725 cellular homeostasis TAS
GO:0030593 neutrophil chemotaxis IEA
GO:0031100 organ regeneration IEA
GO:0032570 response to progesterone stimulus IEA
GO:0033552 response to vitamin B3 IEA
GO:0035684 helper T cell extravasation ISS
GO:0042493 response to drug IEA
GO:0043200 response to amino acid stimulus IEA
GO:0043277 apoptotic cell clearance ISS
GO:0043615 astrocyte cell migration IDA
GO:0045471 response to ethanol IEA
GO:0046677 response to antibiotic IEA
GO:0048010 vascular endothelial growth factor receptor signaling pathway IEA
GO:0048246 macrophage chemotaxis IDA
GO:0048247 lymphocyte chemotaxis IEA
GO:0050806 positive regulation of synaptic transmission IEA
GO:0050870 positive regulation of T cell activation ISS
GO:0051384 response to glucocorticoid stimulus IEA
GO:0051770 positive regulation of nitric-oxide synthase biosynthetic process ISS
GO:0060137 maternal process involved in parturition IEA
GO:0070098 chemokine-mediated signaling pathway IEA
GO:0071222 cellular response to lipopolysaccharide ISS
GO:0071346 cellular response to interferon-gamma ISS
GO:0071347 cellular response to interleukin-1 ISS
GO:0071356 cellular response to tumor necrosis factor ISS

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CCL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18411
NM_002982 Homo sapiens chemokine (C-C motif) ligand 2 (CCL2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.30
$99.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee that the protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OHu18411
Accession Version NM_002982.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 300bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product C-C motif chemokine 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG530064.1 and BC009716.1. This sequence is a reference standard in the RefSeqGene project. On Nov 29, 2004 this sequence version replaced gi:22538812. Summary: This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009716.1, CD522574.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
ATGAAAGTCT CTGCCGCCCT TCTGTGCCTG CTGCTCATAG CAGCCACCTT CATTCCCCAA 
GGGCTCGCTC AGCCAGATGC AATCAATGCC CCAGTCACCT GCTGTTATAA CTTCACCAAT
AGGAAGATCT CAGTGCAGAG GCTCGCGAGC TATAGAAGAA TCACCAGCAG CAAGTGTCCC
AAAGAAGCTG TGATCTTCAA GACCATTGTG GCCAAGGAGA TCTGTGCTGA CCCCAAGCAG
AAGTGGGTTC AGGATTCCAT GGACCACCTG GACAAGCAAA CCCAAACTCC GAAGACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_002973.1
CDS74..373
Misc Feature(1)143..145(+)
Misc Feature(2)152..154(+)
Misc Feature(3)152..154(+)
Misc Feature(4)152..154(+)
Misc Feature(5)164..166(+)
Misc Feature(6)173..346(+)
Misc Feature(7)173..346(+)
Misc Feature(8)179..346(+)
Misc Feature(9)179..298(+)
Misc Feature(10)179..295(+)
Misc Feature(11)179..214(+)
Misc Feature(12)179..181(+)
Misc Feature(13)185..346(+)
Misc Feature(14)191..289(+)
Misc Feature(15)194..196(+)
Misc Feature(16)197..199(+)
Misc Feature(17)212..214(+)
Misc Feature(18)230..337(+)
Misc Feature(19)245..250(+)
Misc Feature(20)245..247(+)
Misc Feature(21)254..256(+)
Misc Feature(22)275..283(+)
Misc Feature(23)287..289(+)
Misc Feature(24)314..316(+)
Misc Feature(25)338..340(+)
Exon (1)1..149
Gene:CCL2
Gene Synonym:
Exon (2)150..267
Gene:CCL2
Gene Synonym:
Exon (3)268..749
Gene:CCL2
Gene Synonym:
Translation
Position Chain Variation Link
3 3 a, g dbSNP:756777436
6 6 a, c dbSNP:767198628
9 9 a, g dbSNP:750066113
19 19 a, g dbSNP:755877280
22 22 g, t dbSNP:779871993
24 24 a, g dbSNP:749627296
37 37 a, c dbSNP:369439824
39 39 a, g dbSNP:779422035
47 47 a, g dbSNP:748665636
52 52 a, g dbSNP:557966833
53 53 g, t dbSNP:773321785
56 56 c, g, t dbSNP:141271352
58 58 c, g dbSNP:776841122
59 59 a, g dbSNP:762750838
62 62 -, t dbSNP:771283629
62 62 c, g dbSNP:763852250
64 64 c, g, t dbSNP:774337603
68 68 a, t dbSNP:370016526
71 71 a, g dbSNP:749900916
84 84 c, t dbSNP:755719349
94 94 c, t dbSNP:766065319
95 95 c, t dbSNP:753449998
97 97 a, g dbSNP:755360396
110 110 a, g dbSNP:144450837
111 111 c, t dbSNP:748658453
116 116 c, g dbSNP:758954928
120 120 c, g dbSNP:778276611
128 128 -, c dbSNP:774898999
129 129 c, t dbSNP:374418259
130 130 c, g dbSNP:747023179
155 155 a, g dbSNP:146604396
166 166 a, g dbSNP:746076530
178 178 c, t dbSNP:4586
188 188 a, g dbSNP:780351792
193 193 c, t dbSNP:371187910
195 195 a, g dbSNP:145056785
196 196 -, at dbSNP:772588061
217 217 c, t dbSNP:138768575
218 218 a, c, g dbSNP:760761240
219 219 c, t dbSNP:148937652
226 226 c, t dbSNP:759111149
233 233 a, g dbSNP:764759504
236 236 a, t dbSNP:752406037
238 238 c, t dbSNP:143644230
278 278 a, g dbSNP:148285031
284 284 a, g dbSNP:190996557
298 298 c, t dbSNP:750956211
303 303 a, g dbSNP:756136784
304 304 a, c dbSNP:766548953
308 308 a, g dbSNP:754083053
313 313 a, g dbSNP:755304641
325 325 a, g dbSNP:779304120
332 332 a, g dbSNP:746763384
333 333 a, t dbSNP:757082330
335 335 a, g dbSNP:780976524
339 339 a, c dbSNP:745882578
348 348 a, g dbSNP:563897168
355 355 c, t dbSNP:769152199
363 363 c, t dbSNP:199565451
364 364 a, g dbSNP:368861512
375 375 -, cact dbSNP:760747031
383 383 c, t dbSNP:376859120
385 385 a, g dbSNP:768257187
388 388 a, g dbSNP:773995779
390 390 a, c dbSNP:762024084
391 391 c, g dbSNP:763076758
407 407 a, t dbSNP:373290550
411 411 -, t dbSNP:764415777
415 415 g, t dbSNP:181021073
417 417 c, g dbSNP:761285800
419 419 c, t dbSNP:766347726
424 424 c, t dbSNP:753924842
438 438 c, t dbSNP:13900
476 476 c, t dbSNP:549055140
545 545 g, t dbSNP:186178409
597 597 a, c dbSNP:191032356
598 598 c, t dbSNP:2215182
642 642 a, c dbSNP:528328195
655 655 a, t dbSNP:75284178
656 656 -, t dbSNP:770114076
657 657 a, t dbSNP:75433364
657 657 -, t dbSNP:536851983
662 662 c, t dbSNP:532809366
665 665 a, g dbSNP:777894448
722 722 c, t dbSNP:552570373

Target ORF information:

RefSeq Version NM_002982
Organism Homo sapiens (human)
Definition Homo sapiens chemokine (C-C motif) ligand 2 (CCL2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002982

ORF Insert Sequence:

1
61
121
181
241
ATGAAAGTCT CTGCCGCCCT TCTGTGCCTG CTGCTCATAG CAGCCACCTT CATTCCCCAA 
GGGCTCGCTC AGCCAGATGC AATCAATGCC CCAGTCACCT GCTGTTATAA CTTCACCAAT
AGGAAGATCT CAGTGCAGAG GCTCGCGAGC TATAGAAGAA TCACCAGCAG CAAGTGTCCC
AAAGAAGCTG TGATCTTCAA GACCATTGTG GCCAAGGAGA TCTGTGCTGA CCCCAAGCAG
AAGTGGGTTC AGGATTCCAT GGACCACCTG GACAAGCAAA CCCAAACTCC GAAGACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

MCP-1and IL-1beta expression in the myocardia of two young patients with Type 1 diabetes mellitus and fatal diabetic ketoacidosis
Exp. Mol. Pathol. 96 (1), 71-79 (2014)
Niu J, Gilliland MG, Jin Z, Kolattukudy PE and Hoffman WH.


book

Chemokine-chemokine receptor CCL2-CCR2 and CX3CL1-CX3CR1 axis may play a role in the aggravated inflammation in primary biliary cirrhosis
Dig. Dis. Sci. 59 (2), 358-364 (2014)
Sasaki M, Miyakoshi M, Sato Y and Nakanuma Y.


book

[Association of chemokines and their receptors genes polymorphisms with risk of myocardial infarction]
Zhonghua Yi Xue Yi Chuan Xue Za Zhi 30 (5), 601-607 (2013)
Xu X, Wang LH, Liu HB, Xu CF, Zhang P, Yong F and Shi YP.


book

Expression of CC chemokines CCL2, CCL5, and CCL11 is associated with duration of disease and complications in type-1 diabetes: a study on Iranian diabetic patients
Clin. Lab. 59 (9-10), 993-1001 (2013)
Jamali Z, Nazari M, Khoramdelazad H, Hakimizadeh E, Mahmoodi M, Karimabad MN, Hassanshahi G, Rezaeian M, Balaei P, Darakhshan S and Poor NM.


book

Association of inflammatory gene polymorphisms and conventional risk factors with arterial stiffness by age
J Epidemiol 23 (6), 457-465 (2013)
Kheradmand M, Niimura H, Kuwabara K, Nakahata N, Nakamura A, Ogawa S, Mantjoro EM, Shimatani K, Nerome Y, Owaki T, Kusano K and Takezaki T.


book

Assignment of the human small inducible cytokine A2 gene, SCYA2 (encoding JE or MCP-1), to 17q11.2-12: evolutionary relatedness of cytokines clustered at the same locus
Genomics 10 (2), 489-492 (1991)
Rollins BJ, Morton CC, Ledbetter DH, Eddy RL Jr and Shows TB.


book

Modeling the three-dimensional structure of the monocyte chemo-attractant and activating protein MCAF/MCP-1 on the basis of the solution structure of interleukin-8
Protein Eng. 4 (3), 263-269 (1991)
Gronenborn AM and Clore GM.


book

Localization of monocyte chemotactic protein-1 gene (SCYA2) to human chromosome 17q11.2-q21.1
Genomics 9 (1), 200-203 (1991)
Mehrabian M, Sparkes RS, Mohandas T, Fogelman AM and Lusis AJ.


book

Human monocyte chemoattractant protein-1 (MCP-1)
Adv. Exp. Med. Biol. 305, 47-56 (1991)
Yoshimura T and Leonard EJ.


book

Identification of the monocyte chemotactic protein from human osteosarcoma cells and monocytes: detection of a novel N-terminally processed form
Biochem. Biophys. Res. Commun. 167 (3), 904-909 (1990)
Decock B, Conings R, Lenaerts JP, Billiau A and Van Damme J.


 
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