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CCL2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CCL2
Entrez Gene ID 6347
Full Name chemokine (C-C motif) ligand 2
Synonyms GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, SCYA2, SMC-CF
General protein information
Preferred Names
C-C motif chemokine 2
Names
C-C motif chemokine 2
small-inducible cytokine A2
monocyte secretory protein JE
monocyte chemotactic protein 1
monocyte chemoattractant protein 1
monocyte chemoattractant protein-1
monocyte chemotactic and activating factor
small inducible cytokine subfamily A (Cys-Cys), member 2
small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q11.2-q12

Summary This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]. lac of sum
Disorder MIM:

158105

Disorder Html: {HIV-1, resistance to}, 609423 (3); {Spina bifida, susceptibility

mRNA and Protein(s)

mRNA Protein Name
NM_002982 NP_002973 C-C motif chemokine 2 precursor



Homo sapiens (human) CCL2 NP_002973.1
Pan troglodytes (chimpanzee) CCL2 XP_001174551.1
Macaca mulatta (Rhesus monkey) CCL2 NP_001027993.1
Canis lupus familiaris (dog) CCL2 NP_001003297.1
Bos taurus (cattle) CCL2 NP_776431.1
Mus musculus (house mouse) Ccl12 NP_035461.2
Rattus norvegicus (Norway rat) Ccl12 NP_001099292.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CCL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18411
NM_002982 Homo sapiens chemokine (C-C motif) ligand 2 (CCL2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18411
Clone ID Related Accession (Same CDS sequence) NM_002982
Accession Version NM_002982.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 300bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product C-C motif chemokine 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG530064.1 and BC009716.1. This sequence is a reference standard in the RefSeqGene project. On Nov 29, 2004 this sequence version replaced gi:22538812. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009716.1, CD522574.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
ATGAAAGTCT CTGCCGCCCT TCTGTGCCTG CTGCTCATAG CAGCCACCTT CATTCCCCAA 
GGGCTCGCTC AGCCAGATGC AATCAATGCC CCAGTCACCT GCTGTTATAA CTTCACCAAT
AGGAAGATCT CAGTGCAGAG GCTCGCGAGC TATAGAAGAA TCACCAGCAG CAAGTGTCCC
AAAGAAGCTG TGATCTTCAA GACCATTGTG GCCAAGGAGA TCTGTGCTGA CCCCAAGCAG
AAGTGGGTTC AGGATTCCAT GGACCACCTG GACAAGCAAA CCCAAACTCC GAAGACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002973.1
CDS74..373
Misc Feature(1)143..145(+)
Misc Feature(2)152..154(+)
Misc Feature(3)152..154(+)
Misc Feature(4)152..154(+)
Misc Feature(5)164..166(+)
Misc Feature(6)173..346(+)
Misc Feature(7)173..346(+)
Misc Feature(8)179..346(+)
Misc Feature(9)179..298(+)
Misc Feature(10)179..295(+)
Misc Feature(11)179..214(+)
Misc Feature(12)179..181(+)
Misc Feature(13)185..346(+)
Misc Feature(14)191..289(+)
Misc Feature(15)194..196(+)
Misc Feature(16)197..199(+)
Misc Feature(17)212..214(+)
Misc Feature(18)230..337(+)
Misc Feature(19)245..250(+)
Misc Feature(20)245..247(+)
Misc Feature(21)254..256(+)
Misc Feature(22)275..283(+)
Misc Feature(23)287..289(+)
Misc Feature(24)314..316(+)
Misc Feature(25)338..340(+)
Exon (1)1..149
Gene:CCL2
Gene Synonym:
Exon (2)150..267
Gene:CCL2
Gene Synonym:
Exon (3)268..749
Gene:CCL2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002982
Organism Homo sapiens (human)
Definition Homo sapiens chemokine (C-C motif) ligand 2 (CCL2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002982

ORF Insert Sequence:

1
61
121
181
241
ATGAAAGTCT CTGCCGCCCT TCTGTGCCTG CTGCTCATAG CAGCCACCTT CATTCCCCAA 
GGGCTCGCTC AGCCAGATGC AATCAATGCC CCAGTCACCT GCTGTTATAA CTTCACCAAT
AGGAAGATCT CAGTGCAGAG GCTCGCGAGC TATAGAAGAA TCACCAGCAG CAAGTGTCCC
AAAGAAGCTG TGATCTTCAA GACCATTGTG GCCAAGGAGA TCTGTGCTGA CCCCAAGCAG
AAGTGGGTTC AGGATTCCAT GGACCACCTG GACAAGCAAA CCCAAACTCC GAAGACTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

MCP-1and IL-1beta expression in the myocardia of two young patients with Type 1 diabetes mellitus and fatal diabetic ketoacidosis
Exp. Mol. Pathol. 96 (1), 71-79 (2014)
Niu J, Gilliland MG, Jin Z, Kolattukudy PE and Hoffman WH.


book

Chemokine-chemokine receptor CCL2-CCR2 and CX3CL1-CX3CR1 axis may play a role in the aggravated inflammation in primary biliary cirrhosis
Dig. Dis. Sci. 59 (2), 358-364 (2014)
Sasaki M, Miyakoshi M, Sato Y and Nakanuma Y.


book

[Association of chemokines and their receptors genes polymorphisms with risk of myocardial infarction]
Zhonghua Yi Xue Yi Chuan Xue Za Zhi 30 (5), 601-607 (2013)
Xu X, Wang LH, Liu HB, Xu CF, Zhang P, Yong F and Shi YP.


book

Expression of CC chemokines CCL2, CCL5, and CCL11 is associated with duration of disease and complications in type-1 diabetes: a study on Iranian diabetic patients
Clin. Lab. 59 (9-10), 993-1001 (2013)
Jamali Z, Nazari M, Khoramdelazad H, Hakimizadeh E, Mahmoodi M, Karimabad MN, Hassanshahi G, Rezaeian M, Balaei P, Darakhshan S and Poor NM.


book

Association of inflammatory gene polymorphisms and conventional risk factors with arterial stiffness by age
J Epidemiol 23 (6), 457-465 (2013)
Kheradmand M, Niimura H, Kuwabara K, Nakahata N, Nakamura A, Ogawa S, Mantjoro EM, Shimatani K, Nerome Y, Owaki T, Kusano K and Takezaki T.


book

Assignment of the human small inducible cytokine A2 gene, SCYA2 (encoding JE or MCP-1), to 17q11.2-12: evolutionary relatedness of cytokines clustered at the same locus
Genomics 10 (2), 489-492 (1991)
Rollins BJ, Morton CC, Ledbetter DH, Eddy RL Jr and Shows TB.


book

Modeling the three-dimensional structure of the monocyte chemo-attractant and activating protein MCAF/MCP-1 on the basis of the solution structure of interleukin-8
Protein Eng. 4 (3), 263-269 (1991)
Gronenborn AM and Clore GM.


book

Localization of monocyte chemotactic protein-1 gene (SCYA2) to human chromosome 17q11.2-q21.1
Genomics 9 (1), 200-203 (1991)
Mehrabian M, Sparkes RS, Mohandas T, Fogelman AM and Lusis AJ.


book

Human monocyte chemoattractant protein-1 (MCP-1)
Adv. Exp. Med. Biol. 305, 47-56 (1991)
Yoshimura T and Leonard EJ.


book

Identification of the monocyte chemotactic protein from human osteosarcoma cells and monocytes: detection of a novel N-terminally processed form
Biochem. Biophys. Res. Commun. 167 (3), 904-909 (1990)
Decock B, Conings R, Lenaerts JP, Billiau A and Van Damme J.


 
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