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CCL4 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CCL4
Entrez Gene ID 6351
Full Name chemokine (C-C motif) ligand 4
Synonyms ACT2, AT744.1, G-26, HC21, LAG-1, LAG1, MIP-1-beta, MIP1B, MIP1B1, SCYA2, SCYA4
General protein information
Preferred Names
C-C motif chemokine 4
Names
C-C motif chemokine 4
PAT 744
SIS-gamma
MIP-1-beta(1-69)
secreted protein G-26
T-cell activation protein 2
G-26 T-lymphocyte-secreted protein
lymphocyte activation gene 1 protein
macrophage inflammatory protein 1-beta
small inducible cytokine A4 (homologous to mouse Mip-1b)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q12

Summary The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. The encoded protein is secreted and has chemokinetic and inflammatory functions. [provided by RefSeq, Dec 2012]. lac of sum
Disorder MIM:

182284

Disorder Html:

mRNA and Protein(s)

mRNA Protein Name
NM_002984 NP_002975 C-C motif chemokine 4 precursor

KEGG
hsa04060 Cytokine-cytokine receptor interaction
hsa04620 Toll-like receptor signaling pathway
hsa04623 Cytosolic DNA-sensing pathway
hsa04062 Chemokine signaling pathway
hsa05132 Salmonella infection
hsa04064 NF-kappa B signaling pathway
Reactome
R-HSA-162582 Signal Transduction
R-HSA-500792 GPCR ligand binding
R-HSA-372790 Signaling by GPCR
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-380108 Chemokine receptors bind chemokines
R-HSA-375276 Peptide ligand-binding receptors
Pathway Interaction Database
il12_2pathway IL12-mediated signaling events
WikiPathways
WP75 Toll-like receptor signaling pathway
WP1449 Regulation of toll-like receptor signaling pathway


Homo sapiens (human) CCL4 NP_002975.1
Pan troglodytes (chimpanzee) CCL4 XP_001173914.1
Macaca mulatta (Rhesus monkey) CCL4L1 NP_001028045.1
Macaca mulatta (Rhesus monkey) LOC100430627 XP_002800427.1
Macaca mulatta (Rhesus monkey) LOC100426632 XP_002800713.1
Macaca mulatta (Rhesus monkey) LOC100423861 XP_002800704.1
Canis lupus familiaris (dog) CCL4 NP_001005250.1
Bos taurus (cattle) CCL4 NP_001068615.1
Mus musculus (house mouse) Ccl4 NP_038680.1
Rattus norvegicus (Norway rat) Ccl4 NP_446310.1
Gallus gallus (chicken) CCL4 NP_990051.1

Component

ID Name Evidence
GO:0005576 extracellular region EXP
GO:0005615 extracellular space IEA

Function

ID Name Evidence
GO:0004716 receptor signaling protein tyrosine kinase activity TAS
GO:0005125 cytokine activity TAS
GO:0008009 chemokine activity IEA

Process

ID Name Evidence
GO:0006928 cellular component movement TAS
GO:0006935 chemotaxis IEA
GO:0006954 inflammatory response IEA
GO:0006955 immune response IEA
GO:0007155 cell adhesion TAS
GO:0007163 establishment or maintenance of cell polarity TAS
GO:0007165 signal transduction TAS
GO:0007267 cell-cell signaling TAS
GO:0009615 response to virus TAS
GO:0019079 viral genome replication TAS

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CCL4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCL4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu60429 NM_002984 Homo sapiens chemokine (C-C motif) ligand 4 (CCL4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $99.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OHu60429
Accession Version NM_002984.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 279bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product C-C motif chemokine 4 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY766446.1. This sequence is a reference standard in the RefSeqGene project. On Jan 17, 2015 this sequence version replaced gi:90704850. Summary: The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. The encoded protein is secreted and has chemokinetic and inflammatory functions. [provided by RefSeq, Dec 2012]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY766446.1, CD521723.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
ATGAAGCTCT GCGTGACTGT CCTGTCTCTC CTCATGCTAG TAGCTGCCTT CTGCTCTCCA 
GCGCTCTCAG CACCAATGGG CTCAGACCCT CCCACCGCCT GCTGCTTTTC TTACACCGCG
AGGAAGCTTC CTCGCAACTT TGTGGTAGAT TACTATGAGA CCAGCAGCCT CTGCTCCCAG
CCAGCTGTGG TATTCCAAAC CAAAAGAAGC AAGCAAGTCT GTGCTGATCC CAGTGAATCC
TGGGTCCAGG AGTACGTGTA TGACCTGGAA CTGAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_002975.1
CDS80..358
Misc Feature(1)53..55(+)
Misc Feature(2)152..154(+)
Misc Feature(3)179..349(+)
Misc Feature(4)179..349(+)
Misc Feature(5)185..349(+)
Misc Feature(6)185..301(+)
Misc Feature(7)185..298(+)
Misc Feature(8)185..220(+)
Misc Feature(9)191..349(+)
Misc Feature(10)197..292(+)
Misc Feature(11)236..340(+)
Misc Feature(12)248..253(+)
Misc Feature(13)278..286(+)
Misc Feature(14)650..655(+)
Exon (1)1..155
Gene:CCL4
Gene Synonym:
Exon (2)156..270
Gene:CCL4
Gene Synonym:
Exon (3)271..667
Gene:CCL4
Gene Synonym:
Translation
Position Chain Variation Link
17 17 a, g dbSNP:2905541
35 35 g, t dbSNP:749163324
44 44 a, c, g dbSNP:768274643
50 50 c, g dbSNP:137963281
52 52 c, g dbSNP:771865798
68 68 g, t dbSNP:1049750
73 73 c, g dbSNP:761153872
75 75 a, g dbSNP:372846688
78 78 c, t dbSNP:777243507
86 86 c, t dbSNP:555952117
91 91 a, c, g, t dbSNP:199619169
92 92 a, g dbSNP:758412463
94 94 c, g dbSNP:149488954
103 103 a, c, g dbSNP:146515425
105 105 c, t dbSNP:779705125
109 109 c, t dbSNP:749317304
112 112 c, t dbSNP:754879682
113 113 a, g dbSNP:1049752
125 125 a, g dbSNP:370817313
127 127 c, t dbSNP:62079739
131 131 c, t dbSNP:368788496
138 138 c, t dbSNP:1130750
141 141 c, t dbSNP:143966312
142 142 a, g dbSNP:1130755
144 144 c, t dbSNP:556935273
145 145 c, g, t dbSNP:776854056
150 150 a, c dbSNP:765673337
155 155 a, g dbSNP:150142971
156 156 c, t dbSNP:758405029
175 175 c, t dbSNP:777786306
176 176 a, g dbSNP:751043350
189 189 c, g, t dbSNP:756740495
191 191 -, a dbSNP:772377163
191 191 a, c, t dbSNP:376875441
193 193 c, t dbSNP:770188150
196 196 c, t dbSNP:1719146
197 197 a, g dbSNP:200881527
198 198 c, t dbSNP:529554188
199 199 a, g dbSNP:774792725
202 202 a, g dbSNP:761832053
206 206 c, g dbSNP:149276903
207 207 a, t dbSNP:773355755
210 210 a, c dbSNP:200124119
212 212 c, t dbSNP:560093309
213 213 a, g, t dbSNP:528656426
226 226 a, g dbSNP:371362421
231 231 a, g dbSNP:751471947
234 234 a, g dbSNP:756755581
235 235 a, t dbSNP:780727741
240 240 c, t dbSNP:750097760
242 242 a, c dbSNP:755772909
244 244 c, t dbSNP:779627759
246 246 g, t dbSNP:3210001
258 258 a, g dbSNP:146953505
261 261 c, t dbSNP:755299219
269 269 c, g dbSNP:137950182
271 271 a, g dbSNP:202017534
279 279 a, c, t dbSNP:771093072
280 280 c, g dbSNP:746063217
283 283 a, g dbSNP:201503735
287 287 a, g dbSNP:1130760
287 287 a, g dbSNP:768389694
289 289 a, c, g dbSNP:546176473
291 291 a, g dbSNP:767516104
293 293 c, g dbSNP:772985242
301 301 c, t dbSNP:1049804
301 301 c, t dbSNP:760220601
307 307 c, t dbSNP:1049805
307 307 c, t dbSNP:765853749
308 308 c, t dbSNP:566173519
316 316 at, ga dbSNP:386796798
316 316 a, g dbSNP:1049807
317 317 a, t dbSNP:1719152
320 320 g, t dbSNP:753117621
333 333 -, acg dbSNP:768340780
334 334 a, c, t dbSNP:371375580
335 335 a, g dbSNP:374631046
339 339 a, g dbSNP:757459200
340 340 g, t dbSNP:745754686
342 342 a, t dbSNP:781405487
359 359 a, g dbSNP:538011270
360 360 c, g dbSNP:746155669
369 369 c, g dbSNP:770072341
374 374 c, g dbSNP:780094697
377 377 g, t dbSNP:181269866
378 378 g, t dbSNP:771735921
385 385 a, g dbSNP:773184436
388 388 a, g dbSNP:200241576
390 390 a, g dbSNP:367933726
400 400 c, t dbSNP:776123799
402 402 c, t dbSNP:1049811
403 403 a, g dbSNP:372180205
420 420 a, c dbSNP:1049813
420 420 a, g dbSNP:536777553
422 422 a, g dbSNP:1049815
422 422 a, g dbSNP:553393313
450 450 c, g dbSNP:563676929
464 464 c, t dbSNP:1049819
476 476 g, t dbSNP:1049821
481 481 c, g dbSNP:573320537
483 483 c, t dbSNP:1049823
493 493 c, t dbSNP:111543488
494 494 c, t dbSNP:1049824
497 497 a, g dbSNP:1049826
507 507 g, t dbSNP:772045543
514 514 c, t dbSNP:1049828
516 516 a, t dbSNP:545421012
521 521 a, g dbSNP:559251821
525 525 c, t dbSNP:184099447
556 556 a, c dbSNP:544761314
576 576 c, g dbSNP:1049833
582 582 c, g dbSNP:1049834
592 592 a, t dbSNP:143747443
597 597 c, t dbSNP:148133700
611 611 a, g dbSNP:751217142
615 615 a, g dbSNP:1049837
618 618 a, t dbSNP:1049838
624 624 c, t dbSNP:1049839
625 625 a, g dbSNP:1049840
640 640 -, a dbSNP:771695635
661 661 a, g dbSNP:531942592
664 664 a, g dbSNP:759193668

Target ORF information:

RefSeq Version NM_002984
Organism Homo sapiens (human)
Definition Homo sapiens chemokine (C-C motif) ligand 4 (CCL4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002984

ORF Insert Sequence:

1
61
121
181
241
ATGAAGCTCT GCGTGACTGT CCTGTCTCTC CTCATGCTAG TAGCTGCCTT CTGCTCTCCA 
GCGCTCTCAG CACCAATGGG CTCAGACCCT CCCACCGCCT GCTGCTTTTC TTACACCGCG
AGGAAGCTTC CTCGCAACTT TGTGGTAGAT TACTATGAGA CCAGCAGCCT CTGCTCCCAG
CCAGCTGTGG TATTCCAAAC CAAAAGAAGC AAGCAAGTCT GTGCTGATCC CAGTGAATCC
TGGGTCCAGG AGTACGTGTA TGACCTGGAA CTGAACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Effects of beta-hydroxy-beta-methylbutyrate free acid and cold water immersion on expression of CR3 and MIP-1beta following resistance exercise
Am. J. Physiol. Regul. Integr. Comp. Physiol. 306 (7), R483-R489 (2014)
Gonzalez AM, Fragala MS, Jajtner AR, Townsend JR, Wells AJ, Beyer KS, Boone CH, Pruna GJ, Mangine GT, Bohner JD, Fukuda DH, Stout JR and Hoffman JR.


book

Increased expression of CCL4/MIP-1beta in CD8+ cells and CD4+ cells in sarcoidosis
Int J Immunopathol Pharmacol 27 (2), 185-193 (2014)
Barczyk A, Pierzchala E, Caramori G and Sozanska E.


book

Dynamics of chemokine, cytokine, and growth factor serum levels in BRAF-mutant melanoma patients during BRAF inhibitor treatment
J. Immunol. 192 (5), 2505-2513 (2014)
Wilmott JS, Haydu LE, Menzies AM, Lum T, Hyman J, Thompson JF, Hersey P, Kefford RF, Scolyer RA and Long GV.


book

27-Hydroxycholesterol and 7alpha-hydroxycholesterol trigger a sequence of events leading to migration of CCR5-expressing Th1 lymphocytes
Toxicol. Appl. Pharmacol. 274 (3), 462-470 (2014)
Kim SM, Kim BY, Lee SA, Eo SK, Yun Y, Kim CD and Kim K.


book

Human T cell leukaemia virus type 2 tax protein mediates CC-chemokine expression in peripheral blood mononuclear cells via the nuclear factor kappa B canonical pathway
Clin. Exp. Immunol. 175 (1), 92-103 (2014)
Barrios CS, Castillo L, Zhi H, Giam CZ and Beilke MA.


book

The gene encoding the Act-2 cytokine. Genomic structure, HTLV-I/Tax responsiveness of 5' upstream sequences, and chromosomal localization
J. Biol. Chem. 266 (26), 17531-17536 (1991)
Napolitano M, Modi WS, Cevario SJ, Gnarra JR, Seuanez HN and Leonard WJ.


book

Cloning and expression of a lymphocyte activation gene (LAG-1)
Mol. Immunol. 27 (11), 1091-1102 (1990)
Baixeras E, Roman-Roman S, Jitsukawa S, Genevee C, Mechiche S, Viegas-Pequignot E, Hercend T and Triebel F.


book

Two inflammatory mediator cytokine genes are closely linked and variably amplified on chromosome 17q
Nucleic Acids Res. 18 (11), 3261-3270 (1990)
Irving SG, Zipfel PF, Balke J, McBride OW, Morton CC, Burd PR, Siebenlist U and Kelly K.


book

A family of small inducible proteins secreted by leukocytes are members of a new superfamily that includes leukocyte and fibroblast-derived inflammatory agents, growth factors, and indicators of various activation processes
J. Immunol. 142 (2), 679-687 (1989)
Brown KD, Zurawski SM, Mosmann TR and Zurawski G.


book

Identification, cloning, and characterization of an immune activation gene
Proc. Natl. Acad. Sci. U.S.A. 85 (24), 9704-9708 (1988)
Lipes MA, Napolitano M, Jeang KT, Chang NT and Leonard WJ.


 
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