×

CCL20 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CCL20
Entrez Gene ID 6364
Full Name chemokine (C-C motif) ligand 20
Synonyms CKb4, Exodus, LARC, MIP-3-alpha, MIP-3a, MIP3A, SCYA20, ST38
General protein information
Preferred Names
C-C motif chemokine 20
Names
C-C motif chemokine 20
CC chemokine LARC
beta chemokine exodus-1
small-inducible cytokine A20
macrophage inflammatory protein 3 alpha
liver and activation-regulated chemokine
small inducible cytokine subfamily A (Cys-Cys), member 20
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2q36.3

Summary This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]. lac of sum
Disorder MIM:

601960

Disorder Html:

mRNA and Protein(s)

mRNA Protein Name
NM_004591 NP_004582 C-C motif chemokine 20 isoform 1 precursor
NM_001130046 NP_001123518 C-C motif chemokine 20 isoform 2 precursor

KEGG
hsa04060 Cytokine-cytokine receptor interaction
hsa04062 Chemokine signaling pathway
hsa05323 Rheumatoid arthritis
hsa04668 TNF signaling pathway
Reactome
R-HSA-162582 Signal Transduction
R-HSA-500792 GPCR ligand binding
R-HSA-388396 GPCR downstream signaling
R-HSA-418594 G alpha (i) signalling events
R-HSA-372790 Signaling by GPCR
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-380108 Chemokine receptors bind chemokines
R-HSA-375276 Peptide ligand-binding receptors
WikiPathways
WP262 EBV LMP1 signaling


Homo sapiens (human) CCL20 NP_001123518.1
Pan troglodytes (chimpanzee) CCL20 XP_516133.2
Macaca mulatta (Rhesus monkey) CCL20 NP_001028026.1
Canis lupus familiaris (dog) CCL20 NP_001005254.1
Bos taurus (cattle) CCL20 NP_776688.1
Mus musculus (house mouse) Ccl20 NP_058656.1
Rattus norvegicus (Norway rat) Ccl20 NP_062106.1
Gallus gallus (chicken) CCL20 NP_989769.1

Component

ID Name Evidence
GO:0005576 extracellular region EXP
GO:0005576 extracellular region TAS
GO:0005615 extracellular space IEA

Function

ID Name Evidence
GO:0008009 chemokine activity IEA

Process

ID Name Evidence
GO:0006935 chemotaxis IEA
GO:0006954 inflammatory response IEA
GO:0006955 immune response IEA
GO:0007165 signal transduction TAS
GO:0007267 cell-cell signaling TAS
GO:0042466 chemokinesis IEA
GO:0042742 defense response to bacterium IEA

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CCL20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCL20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu09144
NM_004591 Homo sapiens chemokine (C-C motif) ligand 20 (CCL20), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.30
$99.00
OHu09153
NM_001130046 Homo sapiens chemokine (C-C motif) ligand 20 (CCL20), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.30
$99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu09144
Accession Version NM_004591.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 291bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product C-C motif chemokine 20 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D17012.1, D86955.1, U77035.1 and U64197.1. On Jul 12, 2008 this sequence version replaced gi:4759075. Summary: This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 12949249 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: CD523297.1, D86955.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
ATGTGCTGTA CCAAGAGTTT GCTCCTGGCT GCTTTGATGT CAGTGCTGCT ACTCCACCTC 
TGCGGCGAAT CAGAAGCAGC AAGCAACTTT GACTGCTGTC TTGGATACAC AGACCGTATT
CTTCATCCTA AATTTATTGT GGGCTTCACA CGGCAGCTGG CCAATGAAGG CTGTGACATC
AATGCTATCA TCTTTCACAC AAAGAAAAAG TTGTCTGTGT GCGCAAATCC AAAACAGACT
TGGGTGAAAT ATATTGTGCG TCTCCTCAGT AAAAAAGTCA AGAACATGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_004582.1
CDS71..361
Misc Feature(1)41..43(+)
Misc Feature(2)158..340(+)
Misc Feature(3)161..172(+)
Misc Feature(4)164..340(+)
Misc Feature(5)170..340(+)
Misc Feature(6)170..292(+)
Misc Feature(7)170..289(+)
Misc Feature(8)170..205(+)
Misc Feature(9)176..340(+)
Misc Feature(10)182..283(+)
Misc Feature(11)221..331(+)
Misc Feature(12)239..244(+)
Misc Feature(13)269..277(+)
Exon (1)1..146
Gene:CCL20
Gene Synonym:
Exon (2)147..261
Gene:CCL20
Gene Synonym:
Exon (3)262..339
Gene:CCL20
Gene Synonym:
Exon (4)340..842
Gene:CCL20
Gene Synonym:
Translation
Position Chain Variation Link
13 13 c, t dbSNP:574701262
32 32 a, g dbSNP:542065571
36 36 a, g dbSNP:767249258
52 52 a, g dbSNP:200671152
59 59 c, g dbSNP:75547135
65 65 a, g dbSNP:779095378
67 67 a, g, t dbSNP:376118694
70 70 c, t dbSNP:778149292
78 78 g, t dbSNP:528011058
79 79 c, t dbSNP:771446659
83 83 a, c dbSNP:774606083
86 86 a, g dbSNP:746428450
91 91 a, g dbSNP:546455394
96 96 c, t dbSNP:775124124
111 111 c, t dbSNP:762365734
124 124 a, c dbSNP:763536188
127 127 c, t dbSNP:774014346
128 128 c, t dbSNP:761413460
133 133 c, t dbSNP:767339095
136 136 c, t dbSNP:750159420
137 137 a, g dbSNP:138641525
138 138 a, t dbSNP:766218950
150 150 c, t dbSNP:764313330
153 153 -, g dbSNP:768443077
176 176 c, t dbSNP:144260473
178 178 c, t dbSNP:757613186
185 185 c, t dbSNP:781267345
186 186 a, g dbSNP:750593133
197 197 c, t dbSNP:756456074
199 199 g, t dbSNP:780425512
206 206 a, g dbSNP:749833569
209 209 a, g dbSNP:1049617
212 212 a, g dbSNP:535020054
217 217 c, t dbSNP:75543153
221 221 c, t dbSNP:747651092
222 222 a, g dbSNP:771540021
227 227 c, g dbSNP:761561150
234 234 a, g dbSNP:191551340
247 247 a, c dbSNP:62190019
250 250 c, t dbSNP:770664886
252 252 a, g dbSNP:148327362
257 257 a, g dbSNP:374526713
260 260 a, c, g dbSNP:758012880
262 262 c, g dbSNP:746684972
285 285 c, t dbSNP:770599570
287 287 a, g dbSNP:776627370
289 289 g, t dbSNP:745654288
292 292 c, g, t dbSNP:150823624
293 293 a, g dbSNP:534991528
298 298 c, t dbSNP:767532562
307 307 a, c, g dbSNP:773319304
310 310 c, t dbSNP:766616352
323 323 a, g dbSNP:368201222
329 329 c, t dbSNP:755346614
330 330 a, g dbSNP:765540742
384 384 a, g dbSNP:776857675
386 386 a, g dbSNP:759717525
404 404 a, g dbSNP:150460518
425 425 a, t dbSNP:531262731
654 654 a, g dbSNP:549639591
662 662 -, g dbSNP:756829217
708 708 g, t dbSNP:189256502
713 713 -, a dbSNP:200141595
713 713 a, t dbSNP:192889020
714 714 a, t dbSNP:185328021
729 729 a, g dbSNP:188367228
756 756 a, t dbSNP:538599471
774 774 a, g dbSNP:181269900
819 819 -, aaag dbSNP:778294919
829 829 a, c dbSNP:116536511

Target ORF information:

RefSeq Version NM_004591
Organism Homo sapiens (human)
Definition Homo sapiens chemokine (C-C motif) ligand 20 (CCL20), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004591

ORF Insert Sequence:

1
61
121
181
241
ATGTGCTGTA CCAAGAGTTT GCTCCTGGCT GCTTTGATGT CAGTGCTGCT ACTCCACCTC 
TGCGGCGAAT CAGAAGCAGC AAGCAACTTT GACTGCTGTC TTGGATACAC AGACCGTATT
CTTCATCCTA AATTTATTGT GGGCTTCACA CGGCAGCTGG CCAATGAAGG CTGTGACATC
AATGCTATCA TCTTTCACAC AAAGAAAAAG TTGTCTGTGT GCGCAAATCC AAAACAGACT
TGGGTGAAAT ATATTGTGCG TCTCCTCAGT AAAAAAGTCA AGAACATGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09153
Accession Version NM_001130046.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 288bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product C-C motif chemokine 20 isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC020698.1, U77035.1 and U64197.1. Summary: This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]. Transcript Variant: This variant (2) uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 12949249 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC020698.1, BG534134.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
ATGTGCTGTA CCAAGAGTTT GCTCCTGGCT GCTTTGATGT CAGTGCTGCT ACTCCACCTC 
TGCGGCGAAT CAGAAGCAAG CAACTTTGAC TGCTGTCTTG GATACACAGA CCGTATTCTT
CATCCTAAAT TTATTGTGGG CTTCACACGG CAGCTGGCCA ATGAAGGCTG TGACATCAAT
GCTATCATCT TTCACACAAA GAAAAAGTTG TCTGTGTGCG CAAATCCAAA ACAGACTTGG
GTGAAATATA TTGTGCGTCT CCTCAGTAAA AAAGTCAAGA ACATGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001123518.1
CDS71..358
Misc Feature(1)41..43(+)
Misc Feature(2)155..337(+)
Misc Feature(3)158..169(+)
Misc Feature(4)161..337(+)
Misc Feature(5)167..337(+)
Misc Feature(6)167..289(+)
Misc Feature(7)167..286(+)
Misc Feature(8)167..202(+)
Misc Feature(9)173..337(+)
Misc Feature(10)179..280(+)
Misc Feature(11)218..328(+)
Misc Feature(12)236..241(+)
Misc Feature(13)266..274(+)
Exon (1)1..146
Gene:CCL20
Gene Synonym:
Exon (2)147..258
Gene:CCL20
Gene Synonym:
Exon (3)259..336
Gene:CCL20
Gene Synonym:
Exon (4)337..839
Gene:CCL20
Gene Synonym:
Translation
Position Chain Variation Link
13 13 c, t dbSNP:574701262
32 32 a, g dbSNP:542065571
36 36 a, g dbSNP:767249258
52 52 a, g dbSNP:200671152
59 59 c, g dbSNP:75547135
65 65 a, g dbSNP:779095378
67 67 a, g, t dbSNP:376118694
70 70 c, t dbSNP:778149292
78 78 g, t dbSNP:528011058
79 79 c, t dbSNP:771446659
83 83 a, c dbSNP:774606083
86 86 a, g dbSNP:746428450
91 91 a, g dbSNP:546455394
96 96 c, t dbSNP:775124124
111 111 c, t dbSNP:762365734
124 124 a, c dbSNP:763536188
127 127 c, t dbSNP:774014346
128 128 c, t dbSNP:761413460
133 133 c, t dbSNP:767339095
136 136 c, t dbSNP:750159420
137 137 a, g dbSNP:138641525
138 138 a, t dbSNP:766218950
147 147 c, t dbSNP:764313330
150 150 -, g dbSNP:768443077
173 173 c, t dbSNP:144260473
175 175 c, t dbSNP:757613186
182 182 c, t dbSNP:781267345
183 183 a, g dbSNP:750593133
194 194 c, t dbSNP:756456074
196 196 g, t dbSNP:780425512
203 203 a, g dbSNP:749833569
206 206 a, g dbSNP:1049617
209 209 a, g dbSNP:535020054
214 214 c, t dbSNP:75543153
218 218 c, t dbSNP:747651092
219 219 a, g dbSNP:771540021
224 224 c, g dbSNP:761561150
231 231 a, g dbSNP:191551340
244 244 a, c dbSNP:62190019
247 247 c, t dbSNP:770664886
249 249 a, g dbSNP:148327362
254 254 a, g dbSNP:374526713
257 257 a, c, g dbSNP:758012880
259 259 c, g dbSNP:746684972
282 282 c, t dbSNP:770599570
284 284 a, g dbSNP:776627370
286 286 g, t dbSNP:745654288
289 289 c, g, t dbSNP:150823624
290 290 a, g dbSNP:534991528
295 295 c, t dbSNP:767532562
304 304 a, c, g dbSNP:773319304
307 307 c, t dbSNP:766616352
320 320 a, g dbSNP:368201222
326 326 c, t dbSNP:755346614
327 327 a, g dbSNP:765540742
381 381 a, g dbSNP:776857675
383 383 a, g dbSNP:759717525
401 401 a, g dbSNP:150460518
422 422 a, t dbSNP:531262731
651 651 a, g dbSNP:549639591
659 659 -, g dbSNP:756829217
705 705 g, t dbSNP:189256502
710 710 -, a dbSNP:200141595
710 710 a, t dbSNP:192889020
711 711 a, t dbSNP:185328021
726 726 a, g dbSNP:188367228
753 753 a, t dbSNP:538599471
771 771 a, g dbSNP:181269900
816 816 -, aaag dbSNP:778294919
826 826 a, c dbSNP:116536511

Target ORF information:

RefSeq Version NM_001130046
Organism Homo sapiens (human)
Definition Homo sapiens chemokine (C-C motif) ligand 20 (CCL20), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001130046

ORF Insert Sequence:

1
61
121
181
241
ATGTGCTGTA CCAAGAGTTT GCTCCTGGCT GCTTTGATGT CAGTGCTGCT ACTCCACCTC 
TGCGGCGAAT CAGAAGCAAG CAACTTTGAC TGCTGTCTTG GATACACAGA CCGTATTCTT
CATCCTAAAT TTATTGTGGG CTTCACACGG CAGCTGGCCA ATGAAGGCTG TGACATCAAT
GCTATCATCT TTCACACAAA GAAAAAGTTG TCTGTGTGCG CAAATCCAAA ACAGACTTGG
GTGAAATATA TTGTGCGTCT CCTCAGTAAA AAAGTCAAGA ACATGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

CCL20 and CXCL8 synergize to promote progression and poor survival outcome in patients with colorectal cancer by collaborative induction of the epithelial-mesenchymal transition
Cancer Lett. 348 (1-2), 77-87 (2014)
Cheng XS, Li YF, Tan J, Sun B, Xiao YC, Fang XB, Zhang XF, Li Q, Dong JH, Li M, Qian HH, Yin ZF and Yang ZB.


book

Dermal clusters of mature dendritic cells and T cells are associated with the CCL20/CCR6 chemokine system in chronic psoriasis
J. Invest. Dermatol. 134 (5), 1462-1465 (2014)
Kim TG, Jee H, Fuentes-Duculan J, Wu WH, Byamba D, Kim DS, Kim DY, Lew DH, Yang WI, Krueger JG and Lee MG.


book

miR-21 and its target gene CCL20 are both highly overexpressed in the microenvironment of colorectal tumors: significance of their regulation
Oncol. Rep. 30 (3), 1285-1292 (2013)
Vicinus B, Rubie C, Stegmaier N, Frick VO, Kolsch K, Kauffels A, Ghadjar P, Wagner M and Glanemann M.


book

CCR6/CCL20 chemokine expression profile in distinct colorectal malignancies
Scand. J. Immunol. 78 (3), 298-305 (2013)
Frick VO, Rubie C, Kolsch K, Wagner M, Ghadjar P, Graeber S and Glanemann M.


book

Chemokine (C-C motif) ligand 20, a potential biomarker for Graves' disease, is regulated by osteopontin
PLoS ONE 8 (5), E64277 (2013)
Li X, Qi Y, Ma X, Huang F, Guo H, Jiang X, Hong J, Lin D, Cui B, Ning G, Xu L and Wang S.


book

Many chemokines including CCL20/MIP-3alpha display antimicrobial activity
J. Leukoc. Biol. 74 (3), 448-455 (2003)
Yang D, Chen Q, Hoover DM, Staley P, Tucker KD, Lubkowski J and Oppenheim JJ.


book

Identification of CCR6, the specific receptor for a novel lymphocyte-directed CC chemokine LARC
J. Biol. Chem. 272 (23), 14893-14898 (1997)
Baba M, Imai T, Nishimura M, Kakizaki M, Takagi S, Hieshima K, Nomiyama H and Yoshie O.


book

Cloning and characterization of exodus, a novel beta-chemokine
Blood 89 (9), 3315-3322 (1997)
Hromas R, Gray PW, Chantry D, Godiska R, Krathwohl M, Fife K, Bell GI, Takeda J, Aronica S, Gordon M, Cooper S, Broxmeyer HE and Klemsz MJ.


book

Molecular cloning of a novel human CC chemokine liver and activation-regulated chemokine (LARC) expressed in liver. Chemotactic activity for lymphocytes and gene localization on chromosome 2
J. Biol. Chem. 272 (9), 5846-5853 (1997)
Hieshima K, Imai T, Opdenakker G, Van Damme J, Kusuda J, Tei H, Sakaki Y, Takatsuki K, Miura R, Yoshie O and Nomiyama H.


book

Identification through bioinformatics of two new macrophage proinflammatory human chemokines: MIP-3alpha and MIP-3beta
J. Immunol. 158 (3), 1033-1036 (1997)
Rossi DL, Vicari AP, Franz-Bacon K, McClanahan TK and Zlotnik A.


 
*
*
*
*