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CX3CL1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CX3CL1
Entrez Gene ID 6376
Full Name chemokine (C-X3-C motif) ligand 1
Synonyms ABCD-3, C3Xkine, CXC3, CXC3C, NTN, NTT, SCYD1, fractalkine, neurotactin
General protein information
Preferred Names
fractalkine
Names
fractalkine
C-X3-C motif chemokine 1
small-inducible cytokine D1
CX3C membrane-anchored chemokine
small inducible cytokine subfamily D (Cys-X3-Cys), member-1
small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

16

16q13

Summary lac of sum
Disorder MIM:

601880


mRNA and Protein(s)

mRNA Protein Name
NM_001304392 NP_001291321 fractalkine isoform 2
NM_002996 NP_002987 fractalkine isoform 1 precursor



Homo sapiens (human) CX3CL1 NP_002987.1
Pan troglodytes (chimpanzee) CX3CL1 XP_510989.2
Macaca mulatta (Rhesus monkey) CX3CL1 NP_001028053.1
Canis lupus familiaris (dog) CX3CL1 XP_005617579.1
Bos taurus (cattle) CX3CL1 XP_002694931.1
Mus musculus (house mouse) Cx3cl1 NP_033168.2
Rattus norvegicus (Norway rat) Cx3cl1 NP_604450.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CX3CL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CX3CL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu60433 NM_001304392 Homo sapiens chemokine (C-X3-C motif) ligand 1 (CX3CL1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $139.30
$199.00
OHu23577
NM_002996 Homo sapiens chemokine (C-X3-C motif) ligand 1 (CX3CL1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu60433
Clone ID Related Accession (Same CDS sequence) NM_001304392
Accession Version NM_001304392.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 939bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product fractalkine isoform 2
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY114023.1, CA396869.1, BC001163.1, AC009052.8 and GU047346.1. Transcript Variant: This variant (2) lacks an alternate exon in the 5' end and initiates translation at an alternate start codon compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CA396869.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCAGCATC TGGACCGCCA GGCTGCTGCC CTAACTCGAA ATGGCGGCAC CTTCGAGAAG 
CAGATCGGCG AGGTGAAGCC CAGGACCACC CCTGCCGCCG GGGGAATGGA CGAGTCTGTG
GTCCTGGAGC CCGAAGCCAC AGGCGAAAGC AGTAGCCTGG AGCCGACTCC TTCTTCCCAG
GAAGCACAGA GGGCCCTGGG GACCTCCCCA GAGCTGCCGA CGGGCGTGAC TGGTTCCTCA
GGGACCAGGC TCCCCCCGAC GCCAAAGGCT CAGGATGGAG GGCCTGTGGG CACGGAGCTT
TTCCGAGTGC CTCCCGTCTC CACTGCCGCC ACGTGGCAGA GTTCTGCTCC CCACCAACCT
GGGCCCAGCC TCTGGGCTGA GGCAAAGACC TCTGAGGCCC CGTCCACCCA GGACCCCTCC
ACCCAGGCCT CCACTGCGTC CTCCCCAGCC CCAGAGGAGA ATGCTCCGTC TGAAGGCCAG
CGTGTGTGGG GTCAGGGACA GAGCCCCAGG CCAGAGAACT CTCTGGAGCG GGAGGAGATG
GGTCCCGTGC CAGCGCACAC GGATGCCTTC CAGGACTGGG GGCCTGGCAG CATGGCCCAC
GTCTCTGTGG TCCCTGTCTC CTCAGAAGGG ACCCCCAGCA GGGAGCCAGT GGCTTCAGGC
AGCTGGACCC CTAAGGCTGA GGAACCCATC CATGCCACCA TGGACCCCCA GAGGCTGGGC
GTCCTTATCA CTCCTGTCCC TGACGCCCAG GCTGCCACCC GGAGGCAGGC GGTGGGGCTG
CTGGCCTTCC TTGGCCTCCT CTTCTGCCTG GGGGTGGCCA TGTTCACCTA CCAGAGCCTC
CAGGGCTGCC CTCGAAAGAT GGCAGGAGAG ATGGCGGAGG GCCTTCGCTA CATCCCCCGG
AGCTGTGGTA GTAATTCATA TGTCCTGGTG CCCGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001291321.1
CDS243..1181
Misc Feature(1)63..65(+)
Misc Feature(2)243..287(+)
Exon (1)1..178
Gene:CX3CL1
Gene Synonym:
Exon (2)179..3197
Gene:CX3CL1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001304392
Organism Homo sapiens (human)
Definition Homo sapiens chemokine (C-X3-C motif) ligand 1 (CX3CL1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001304392

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCAGCATC TGGACCGCCA GGCTGCTGCC CTAACTCGAA ATGGCGGCAC CTTCGAGAAG 
CAGATCGGCG AGGTGAAGCC CAGGACCACC CCTGCCGCCG GGGGAATGGA CGAGTCTGTG
GTCCTGGAGC CCGAAGCCAC AGGCGAAAGC AGTAGCCTGG AGCCGACTCC TTCTTCCCAG
GAAGCACAGA GGGCCCTGGG GACCTCCCCA GAGCTGCCGA CGGGCGTGAC TGGTTCCTCA
GGGACCAGGC TCCCCCCGAC GCCAAAGGCT CAGGATGGAG GGCCTGTGGG CACGGAGCTT
TTCCGAGTGC CTCCCGTCTC CACTGCCGCC ACGTGGCAGA GTTCTGCTCC CCACCAACCT
GGGCCCAGCC TCTGGGCTGA GGCAAAGACC TCTGAGGCCC CGTCCACCCA GGACCCCTCC
ACCCAGGCCT CCACTGCGTC CTCCCCAGCC CCAGAGGAGA ATGCTCCGTC TGAAGGCCAG
CGTGTGTGGG GTCAGGGACA GAGCCCCAGG CCAGAGAACT CTCTGGAGCG GGAGGAGATG
GGTCCCGTGC CAGCGCACAC GGATGCCTTC CAGGACTGGG GGCCTGGCAG CATGGCCCAC
GTCTCTGTGG TCCCTGTCTC CTCAGAAGGG ACCCCCAGCA GGGAGCCAGT GGCTTCAGGC
AGCTGGACCC CTAAGGCTGA GGAACCCATC CATGCCACCA TGGACCCCCA GAGGCTGGGC
GTCCTTATCA CTCCTGTCCC TGACGCCCAG GCTGCCACCC GGAGGCAGGC GGTGGGGCTG
CTGGCCTTCC TTGGCCTCCT CTTCTGCCTG GGGGTGGCCA TGTTCACCTA CCAGAGCCTC
CAGGGCTGCC CTCGAAAGAT GGCAGGAGAG ATGGCGGAGG GCCTTCGCTA CATCCCCCGG
AGCTGTGGTA GTAATTCATA TGTCCTGGTG CCCGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23577
Clone ID Related Accession (Same CDS sequence) NM_002996
Accession Version NM_002996.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1194bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product fractalkine isoform 1 precursor
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY114023.1, BC001163.1, AC009052.8 and GU047346.1. On Jan 22, 2015 this sequence version replaced gi:54111253. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY114023.1, BC001163.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGCTCCGA TATCTCTGTC GTGGCTGCTC CGCTTGGCCA CCTTCTGCCA TCTGACTGTC 
CTGCTGGCTG GACAGCACCA CGGTGTGACG AAATGCAACA TCACGTGCAG CAAGATGACA
TCAAAGATAC CTGTAGCTTT GCTCATCCAC TATCAACAGA ACCAGGCATC ATGCGGCAAA
CGCGCAATCA TCTTGGAGAC GAGACAGCAC AGGCTGTTCT GTGCCGACCC GAAGGAGCAA
TGGGTCAAGG ACGCGATGCA GCATCTGGAC CGCCAGGCTG CTGCCCTAAC TCGAAATGGC
GGCACCTTCG AGAAGCAGAT CGGCGAGGTG AAGCCCAGGA CCACCCCTGC CGCCGGGGGA
ATGGACGAGT CTGTGGTCCT GGAGCCCGAA GCCACAGGCG AAAGCAGTAG CCTGGAGCCG
ACTCCTTCTT CCCAGGAAGC ACAGAGGGCC CTGGGGACCT CCCCAGAGCT GCCGACGGGC
GTGACTGGTT CCTCAGGGAC CAGGCTCCCC CCGACGCCAA AGGCTCAGGA TGGAGGGCCT
GTGGGCACGG AGCTTTTCCG AGTGCCTCCC GTCTCCACTG CCGCCACGTG GCAGAGTTCT
GCTCCCCACC AACCTGGGCC CAGCCTCTGG GCTGAGGCAA AGACCTCTGA GGCCCCGTCC
ACCCAGGACC CCTCCACCCA GGCCTCCACT GCGTCCTCCC CAGCCCCAGA GGAGAATGCT
CCGTCTGAAG GCCAGCGTGT GTGGGGTCAG GGACAGAGCC CCAGGCCAGA GAACTCTCTG
GAGCGGGAGG AGATGGGTCC CGTGCCAGCG CACACGGATG CCTTCCAGGA CTGGGGGCCT
GGCAGCATGG CCCACGTCTC TGTGGTCCCT GTCTCCTCAG AAGGGACCCC CAGCAGGGAG
CCAGTGGCTT CAGGCAGCTG GACCCCTAAG GCTGAGGAAC CCATCCATGC CACCATGGAC
CCCCAGAGGC TGGGCGTCCT TATCACTCCT GTCCCTGACG CCCAGGCTGC CACCCGGAGG
CAGGCGGTGG GGCTGCTGGC CTTCCTTGGC CTCCTCTTCT GCCTGGGGGT GGCCATGTTC
ACCTACCAGA GCCTCCAGGG CTGCCCTCGA AAGATGGCAG GAGAGATGGC GGAGGGCCTT
CGCTACATCC CCCGGAGCTG TGGTAGTAAT TCATATGTCC TGGTGCCCGT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002987.1
CDS109..1302
Misc Feature(1)43..45(+)
Misc Feature(2)181..408(+)
Misc Feature(3)181..408(+)
Misc Feature(4)181..378(+)
Misc Feature(5)202..324(+)
Misc Feature(6)205..252(+)
Misc Feature(7)205..213(+)
Misc Feature(8)220..402(+)
Misc Feature(9)268..294(+)
Misc Feature(10)286..381(+)
Misc Feature(11)307..321(+)
Misc Feature(12)409..1131(+)
Misc Feature(13)1123..1128(+)
Misc Feature(14)1132..1194(+)
Exon (1)1..178
Gene:CX3CL1
Gene Synonym:
Exon (2)179..299
Gene:CX3CL1
Gene Synonym:
Exon (3)300..3318
Gene:CX3CL1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002996
Organism Homo sapiens (human)
Definition Homo sapiens chemokine (C-X3-C motif) ligand 1 (CX3CL1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002996

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGCTCCGA TATCTCTGTC GTGGCTGCTC CGCTTGGCCA CCTTCTGCCA TCTGACTGTC 
CTGCTGGCTG GACAGCACCA CGGTGTGACG AAATGCAACA TCACGTGCAG CAAGATGACA
TCAAAGATAC CTGTAGCTTT GCTCATCCAC TATCAACAGA ACCAGGCATC ATGCGGCAAA
CGCGCAATCA TCTTGGAGAC GAGACAGCAC AGGCTGTTCT GTGCCGACCC GAAGGAGCAA
TGGGTCAAGG ACGCGATGCA GCATCTGGAC CGCCAGGCTG CTGCCCTAAC TCGAAATGGC
GGCACCTTCG AGAAGCAGAT CGGCGAGGTG AAGCCCAGGA CCACCCCTGC CGCCGGGGGA
ATGGACGAGT CTGTGGTCCT GGAGCCCGAA GCCACAGGCG AAAGCAGTAG CCTGGAGCCG
ACTCCTTCTT CCCAGGAAGC ACAGAGGGCC CTGGGGACCT CCCCAGAGCT GCCGACGGGC
GTGACTGGTT CCTCAGGGAC CAGGCTCCCC CCGACGCCAA AGGCTCAGGA TGGAGGGCCT
GTGGGCACGG AGCTTTTCCG AGTGCCTCCC GTCTCCACTG CCGCCACGTG GCAGAGTTCT
GCTCCCCACC AACCTGGGCC CAGCCTCTGG GCTGAGGCAA AGACCTCTGA GGCCCCGTCC
ACCCAGGACC CCTCCACCCA GGCCTCCACT GCGTCCTCCC CAGCCCCAGA GGAGAATGCT
CCGTCTGAAG GCCAGCGTGT GTGGGGTCAG GGACAGAGCC CCAGGCCAGA GAACTCTCTG
GAGCGGGAGG AGATGGGTCC CGTGCCAGCG CACACGGATG CCTTCCAGGA CTGGGGGCCT
GGCAGCATGG CCCACGTCTC TGTGGTCCCT GTCTCCTCAG AAGGGACCCC CAGCAGGGAG
CCAGTGGCTT CAGGCAGCTG GACCCCTAAG GCTGAGGAAC CCATCCATGC CACCATGGAC
CCCCAGAGGC TGGGCGTCCT TATCACTCCT GTCCCTGACG CCCAGGCTGC CACCCGGAGG
CAGGCGGTGG GGCTGCTGGC CTTCCTTGGC CTCCTCTTCT GCCTGGGGGT GGCCATGTTC
ACCTACCAGA GCCTCCAGGG CTGCCCTCGA AAGATGGCAG GAGAGATGGC GGAGGGCCTT
CGCTACATCC CCCGGAGCTG TGGTAGTAAT TCATATGTCC TGGTGCCCGT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

High coexpression of CCL2 and CX3CL1 is gender-specifically associated with good prognosis in soft tissue sarcoma patients
Int. J. Cancer 135 (9), 2096-2106 (2014)
Kehlen A, Greither T, Wach S, Nolte E, Kappler M, Bache M, Holzhausen HJ, Lautenschlager C, Gobel S, Wurl P, Immel UD, Agaimy A, Wullich B and Taubert H.


book

Functional analysis of the fractalkine gene promoter in human aortic smooth muscle cells exposed to proinflammatory conditions
FEBS J. 281 (17), 3869-3881 (2014)
Gan AM, Butoi E, Manea A, Pirvulescu MM, Stan D, Simion V, Calin M, Simionescu M and Manduteanu I.


book

Circulating human fractalkine is decreased post-operatively after orthopedic and coronary bypass surgery
In Vivo 28 (2), 185-188 (2014)
Akerfeldt T, Helmersson-Karlqvist J, Gordh T and Larsson A.


book

The M2 polarization of macrophage induced by fractalkine in the endometriotic milieu enhances invasiveness of endometrial stromal cells
Int J Clin Exp Pathol 7 (1), 194-203 (2013)
Wang Y, Fu Y, Xue S, Ai A, Chen H, Lyu Q and Kuang Y.


book

Neuron-glia crosstalk in health and disease: fractalkine and CX3CR1 take centre stage
Open Biol 3 (12), 130181 (2013)
Sheridan GK and Murphy KJ.


book

Identification of CX3CR1. A chemotactic receptor for the human CX3C chemokine fractalkine and a fusion coreceptor for HIV-1
J. Biol. Chem. 273 (37), 23799-23804 (1998)
Combadiere C, Salzwedel K, Smith ED, Tiffany HL, Berger EA and Murphy PM.


book

Human chemokines fractalkine (SCYD1), MDC (SCYA22) and TARC (SCYA17) are clustered on chromosome 16q13
Cytogenet. Cell Genet. 81 (1), 10-11 (1998)
Nomiyama H, Imai T, Kusuda J, Miura R, Callen DF and Yoshie O.


book

Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion
Cell 91 (4), 521-530 (1997)
Imai T, Hieshima K, Haskell C, Baba M, Nagira M, Nishimura M, Kakizaki M, Takagi S, Nomiyama H, Schall TJ and Yoshie O.


book

Neurotactin, a membrane-anchored chemokine upregulated in brain inflammation
Nature 387 (6633), 611-617 (1997)
Pan Y, Lloyd C, Zhou H, Dolich S, Deeds J, Gonzalo JA, Vath J, Gosselin M, Ma J, Dussault B, Woolf E, Alperin G, Culpepper J, Gutierrez-Ramos JC and Gearing D.


book

A new class of membrane-bound chemokine with a CX3C motif
Nature 385 (6617), 640-644 (1997)
Bazan JF, Bacon KB, Hardiman G, Wang W, Soo K, Rossi D, Greaves DR, Zlotnik A and Schall TJ.


 
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