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SDHA cDNA ORF clone, Homo sapiens (human)

Gene Symbol SDHA
Entrez Gene ID 6389
Full Name succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
Synonyms CMD1GG, FP, PGL5, SDH1, SDH2, SDHF
General protein information
Preferred Names
succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
Names
succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
flavoprotein subunit of complex II
succinate dehydrogenase complex flavoprotein subunit
NP_004159.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

5

5p15

Summary This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]. lac of sum
Disorder MIM:

600857

Disorder Html: Leigh syndrome, 256000 (3); Mitochondrial respiratory chain complex

mRNA and Protein(s)

mRNA Protein Name
XM_011514072 XP_011512374 succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform X1
XM_011514073 XP_011512375 succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform X2
XM_005248331 XP_005248388 succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform X3
NM_004168 NP_004159 succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform 1
NM_001294332 NP_001281261 succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform 2



Homo sapiens (human) SDHA NP_004159.2
Pan troglodytes (chimpanzee) SDHA XP_003310656.1
Macaca mulatta (Rhesus monkey) LOC708018 XP_001094170.2
Canis lupus familiaris (dog) SDHA XP_535807.4
Bos taurus (cattle) SDHA NP_776603.1
Mus musculus (house mouse) Sdha NP_075770.1
Rattus norvegicus (Norway rat) Sdha NP_569112.1
Gallus gallus (chicken) SDHA NP_001264327.1
Danio rerio (zebrafish) sdha NP_957204.1
Drosophila melanogaster (fruit fly) SdhA NP_725882.1
Caenorhabditis elegans sdha-1 NP_509446.1
Caenorhabditis elegans sdha-2 NP_492798.1
Arabidopsis thaliana (thale cress) SDH1-1 NP_201477.1
Xenopus (Silurana) tropicalis (western clawed frog) sdha NP_001015989.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SDHA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SDHA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu60440 XM_011514072 PREDICTED: Homo sapiens succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (SDHA), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu60441 XM_011514073 PREDICTED: Homo sapiens succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (SDHA), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu39348 XM_005248331 PREDICTED: Homo sapiens succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (SDHA), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu28692
NM_004168 Homo sapiens succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (SDHA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu28693 NM_001294332 Homo sapiens succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (SDHA), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu60440
Clone ID Related Accession (Same CDS sequence) XM_011514072
Accession Version XM_011514072.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2043bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_006576.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGGGAGGAAT CAATGCTGCT CTGGGGAACA TGGAGGAGGA CAACTGGAGG
TGGCATTTCT ACGACACCGT GAAGGGCTCC GACTGGCTGG GGGACCAGGA TGCCATCCAC
TACATGACGG AGCAGGCCCC CGCCGCCGTG GTCGAGCTAG AAAATTATGG CATGCCGTTT
AGCAGAACTG AAGATGGGAA GATTTATCAG CGTGCATTTG GTGGACAGAG CCTCAAGTTT
GGAAAGGGCG GGCAGGCCCA TCGGTGCTGC TGTGTGGCTG ATCGGACTGG CCACTCGCTA
TTGCACACCT TATATGGAAG GTCTCTGCGA TATGATACCA GCTATTTTGT GGAGTATTTT
GCCTTGGATC TCCTGATGGA GAATGGGGAG TGCCGTGGTG TCATCGCACT GTGCATAGAG
GACGGGTCCA TCCATCGCAT AAGAGCAAAG AACACTGTTG TTGCCACAGG AGGCTACGGG
CGCACCTACT TCAGCTGCAC GTCTGCCCAC ACCAGCACTG GCGACGGCAC GGCCATGATC
ACCAGGGCAG GCCTTCCTTG CCAGGACCTA GAGTTTGTTC AGTTCCACCC TACAGGCATA
TATGGTGCTG GTTGTCTCAT TACGGAAGGA TGTCGTGGAG AGGGAGGCAT TCTCATTAAC
AGTCAAGGCG AAAGGTTTAT GGAGCGATAC GCCCCTGTCG CGAAGGACCT GGCGTCTAGA
GATGTGGTGT CTCGGTCCAT GACTCTGGAG ATCCGAGAAG GAAGAGGCTG TGGCCCTGAG
AAAGATCACG TCTACCTGCA GCTGCACCAC CTACCTCCAG AGCAGCTGGC CACGCGCCTG
CCTGGCATTT CAGAGACAGC CATGATCTTC GCTGGCGTGG ACGTCACGAA GGAGCCGATC
CCTGTCCTCC CCACCGTGCA TTATAACATG GGCGGCATTC CCACCAACTA CAAGGGGCAG
GTCCTGAGGC ACGTGAATGG CCAGGATCAG ATTGTGCCCG GCCTGTACGC CTGTGGGGAG
GCCGCCTGTG CCTCGGTACA TGGTGCCAAC CGCCTCGGGG CAAACTCGCT CTTGGACCTG
GTTGTCTTTG GTCGGGCATG TGCCCTGAGC ATCGAAGAGT CATGCAGGCC TGGAGATAAA
GTCCCTCCAA TTAAACCAAA CGCTGGGGAA GAATCTGTCA TGAATCTTGA CAAATTGAGA
TTTGCTGATG GAAGCATAAG AACATCGGAA CTGCGACTCA GCATGCAGAA GTCAATGCAA
AATCATGCTG CCGTGTTCCG TGTGGGAAGC GTGTTGCAAG AAGGTTGTGG GAAAATCAGC
AAGCTCTATG GAGACCTAAA GCACCTGAAG ACGTTCGACC GGGGAATGGT CTGGAACACG
GACCTGGTGG AGACCCTGGA GCTGCAGAAC CTGATGCTGT GTGCGCTGCA GACCATCTAC
GGAGCAGAGG CACGGAAGGA GTCACGGGGC GCGCATGCCA GGGAAGACTA CAAGGTGCGG
ATTGATGAGT ACGATTACTC CAAGCCCATC CAGGGGCAAC AGAAGAAGCC CTTTGAGGAG
CACTGGAGGA AGCACACCCT GTCCTATGTG GACGTTGGCA CTGGGAAGTG CACAGCCAGA
GAACAACACA TCACACACAG GAAACAGTTG TGCTCATGTG ATGGGGGCCT CAGCACTAGG
AAGGAGCGGA CTATTGGTGC ACGCAGCAGC TTGAATAAAT CTGAAAGTCA CTATGCTGTG
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011512374.1
CDS134..2176
Misc Feature(1)251..2041(+)
Misc Feature(2)386..1546(+)
Misc Feature(3)1667..2026(+)
Translation

Target ORF information:

RefSeq Version XM_011514072
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (SDHA), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011514072

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGGGAGGAAT CAATGCTGCT CTGGGGAACA TGGAGGAGGA CAACTGGAGG
TGGCATTTCT ACGACACCGT GAAGGGCTCC GACTGGCTGG GGGACCAGGA TGCCATCCAC
TACATGACGG AGCAGGCCCC CGCCGCCGTG GTCGAGCTAG AAAATTATGG CATGCCGTTT
AGCAGAACTG AAGATGGGAA GATTTATCAG CGTGCATTTG GTGGACAGAG CCTCAAGTTT
GGAAAGGGCG GGCAGGCCCA TCGGTGCTGC TGTGTGGCTG ATCGGACTGG CCACTCGCTA
TTGCACACCT TATATGGAAG GTCTCTGCGA TATGATACCA GCTATTTTGT GGAGTATTTT
GCCTTGGATC TCCTGATGGA GAATGGGGAG TGCCGTGGTG TCATCGCACT GTGCATAGAG
GACGGGTCCA TCCATCGCAT AAGAGCAAAG AACACTGTTG TTGCCACAGG AGGCTACGGG
CGCACCTACT TCAGCTGCAC GTCTGCCCAC ACCAGCACTG GCGACGGCAC GGCCATGATC
ACCAGGGCAG GCCTTCCTTG CCAGGACCTA GAGTTTGTTC AGTTCCACCC TACAGGCATA
TATGGTGCTG GTTGTCTCAT TACGGAAGGA TGTCGTGGAG AGGGAGGCAT TCTCATTAAC
AGTCAAGGCG AAAGGTTTAT GGAGCGATAC GCCCCTGTCG CGAAGGACCT GGCGTCTAGA
GATGTGGTGT CTCGGTCCAT GACTCTGGAG ATCCGAGAAG GAAGAGGCTG TGGCCCTGAG
AAAGATCACG TCTACCTGCA GCTGCACCAC CTACCTCCAG AGCAGCTGGC CACGCGCCTG
CCTGGCATTT CAGAGACAGC CATGATCTTC GCTGGCGTGG ACGTCACGAA GGAGCCGATC
CCTGTCCTCC CCACCGTGCA TTATAACATG GGCGGCATTC CCACCAACTA CAAGGGGCAG
GTCCTGAGGC ACGTGAATGG CCAGGATCAG ATTGTGCCCG GCCTGTACGC CTGTGGGGAG
GCCGCCTGTG CCTCGGTACA TGGTGCCAAC CGCCTCGGGG CAAACTCGCT CTTGGACCTG
GTTGTCTTTG GTCGGGCATG TGCCCTGAGC ATCGAAGAGT CATGCAGGCC TGGAGATAAA
GTCCCTCCAA TTAAACCAAA CGCTGGGGAA GAATCTGTCA TGAATCTTGA CAAATTGAGA
TTTGCTGATG GAAGCATAAG AACATCGGAA CTGCGACTCA GCATGCAGAA GTCAATGCAA
AATCATGCTG CCGTGTTCCG TGTGGGAAGC GTGTTGCAAG AAGGTTGTGG GAAAATCAGC
AAGCTCTATG GAGACCTAAA GCACCTGAAG ACGTTCGACC GGGGAATGGT CTGGAACACG
GACCTGGTGG AGACCCTGGA GCTGCAGAAC CTGATGCTGT GTGCGCTGCA GACCATCTAC
GGAGCAGAGG CACGGAAGGA GTCACGGGGC GCGCATGCCA GGGAAGACTA CAAGGTGCGG
ATTGATGAGT ACGATTACTC CAAGCCCATC CAGGGGCAAC AGAAGAAGCC CTTTGAGGAG
CACTGGAGGA AGCACACCCT GTCCTATGTG GACGTTGGCA CTGGGAAGTG CACAGCCAGA
GAACAACACA TCACACACAG GAAACAGTTG TGCTCATGTG ATGGGGGCCT CAGCACTAGG
AAGGAGCGGA CTATTGGTGC ACGCAGCAGC TTGAATAAAT CTGAAAGTCA CTATGCTGTG
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60441
Clone ID Related Accession (Same CDS sequence) XM_011514073
Accession Version XM_011514073.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1800bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_006576.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGGGAGGAAT CAATGCTGCT CTGGGGAACA TGGAGGAGGA CAACTGGAGG
TGGCATTTCT ACGACACCGT GAAGGGCTCC GACTGGCTGG GGGACCAGGA TGCCATCCAC
TACATGACGG AGCAGGCCCC CGCCGCCGTG GTCGAGCTAG AAAATTATGG CATGCCGTTT
AGCAGAACTG AAGATGGGAA GATTTATCAG CGTGCATTTG GTGGACAGAG CCTCAAGTTT
GGAAAGGGCG GGCAGGCCCA TCGGTGCTGC TGTGTGGCTG ATCGGACTGG CCACTCGCTA
TTGCACACCT TATATGGAAG GTCTCTGCGA TATGATACCA GCTATTTTGT GGAGTATTTT
GCCTTGGATC TCCTGATGGA GAATGGGGAG TGCCGTGGTG TCATCGCACT GTGCATAGAG
GACGGGTCCA TCCATCGCAT AAGAGCAAAG AACACTGTTG TTGCCACAGG AGGCTACGGG
CGCACCTACT TCAGCTGCAC GTCTGCCCAC ACCAGCACTG GCGACGGCAC GGCCATGATC
ACCAGGGCAG GCCTTCCTTG CCAGGACCTA GAGTTTGTTC AGTTCCACCC TACAGGCATA
TATGGTGCTG GTTGTCTCAT TACGGAAGGA TGTCGTGGAG AGGGAGGCAT TCTCATTAAC
AGTCAAGGCG AAAGGTTTAT GGAGCGATAC GCCCCTGTCG CGAAGGACCT GGCGTCTAGA
GATGTGGTGT CTCGGTCCAT GACTCTGGAG ATCCGAGAAG GAAGAGGCTG TGGCCCTGAG
AAAGATCACG TCTACCTGCA GCTGCACCAC CTACCTCCAG AGCAGCTGGC CACGCGCCTG
CCTGGCATTT CAGAGACAGC CATGATCTTC GCTGGCGTGG ACGTCACGAA GGAGCCGATC
CCTGTCCTCC CCACCGTGCA TTATAACATG GGCGGCATTC CCACCAACTA CAAGGGGCAG
GTCCTGAGGC ACGTGAATGG CCAGGATCAG ATTGTGCCCG GCCTGTACGC CTGTGGGGAG
GCCGCCTGTG CCTCGGTACA TGGTGCCAAC CGCCTCGGGG CAAACTCGCT CTTGGACCTG
GTTGTCTTTG GTCGGGCATG TGCCCTGAGC ATCGAAGAGT CATGCAGGCC TGGAGATAAA
GTCCCTCCAA TTAAACCAAA CGCTGGGGAA GAATCTGTCA TGAATCTTGA CAAATTGAGA
TTTGCTGATG GAAGCATAAG AACATCGGAA CTGCGACTCA GCATGCAGAA GGTGCGGATT
GATGAGTACG ATTACTCCAA GCCCATCCAG GGGCAACAGA AGAAGCCCTT TGAGGAGCAC
TGGAGGAAGC ACACCCTGTC CTATGTGGAC GTTGGCACTG GGAAGTGCAC AGCCAGAGAA
CAACACATCA CACACAGGAA ACAGTTGTGC TCATGTGATG GGGGCCTCAG CACTAGGAAG
GAGCGGACTA TTGGTGCACG CAGCAGCTTG AATAAATCTG AAAGTCACTA TGCTGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011512375.1
CDS134..1933
Misc Feature(1)371..1798(+)
Misc Feature(2)386..1546(+)
Translation

Target ORF information:

RefSeq Version XM_011514073
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (SDHA), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011514073

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGGGAGGAAT CAATGCTGCT CTGGGGAACA TGGAGGAGGA CAACTGGAGG
TGGCATTTCT ACGACACCGT GAAGGGCTCC GACTGGCTGG GGGACCAGGA TGCCATCCAC
TACATGACGG AGCAGGCCCC CGCCGCCGTG GTCGAGCTAG AAAATTATGG CATGCCGTTT
AGCAGAACTG AAGATGGGAA GATTTATCAG CGTGCATTTG GTGGACAGAG CCTCAAGTTT
GGAAAGGGCG GGCAGGCCCA TCGGTGCTGC TGTGTGGCTG ATCGGACTGG CCACTCGCTA
TTGCACACCT TATATGGAAG GTCTCTGCGA TATGATACCA GCTATTTTGT GGAGTATTTT
GCCTTGGATC TCCTGATGGA GAATGGGGAG TGCCGTGGTG TCATCGCACT GTGCATAGAG
GACGGGTCCA TCCATCGCAT AAGAGCAAAG AACACTGTTG TTGCCACAGG AGGCTACGGG
CGCACCTACT TCAGCTGCAC GTCTGCCCAC ACCAGCACTG GCGACGGCAC GGCCATGATC
ACCAGGGCAG GCCTTCCTTG CCAGGACCTA GAGTTTGTTC AGTTCCACCC TACAGGCATA
TATGGTGCTG GTTGTCTCAT TACGGAAGGA TGTCGTGGAG AGGGAGGCAT TCTCATTAAC
AGTCAAGGCG AAAGGTTTAT GGAGCGATAC GCCCCTGTCG CGAAGGACCT GGCGTCTAGA
GATGTGGTGT CTCGGTCCAT GACTCTGGAG ATCCGAGAAG GAAGAGGCTG TGGCCCTGAG
AAAGATCACG TCTACCTGCA GCTGCACCAC CTACCTCCAG AGCAGCTGGC CACGCGCCTG
CCTGGCATTT CAGAGACAGC CATGATCTTC GCTGGCGTGG ACGTCACGAA GGAGCCGATC
CCTGTCCTCC CCACCGTGCA TTATAACATG GGCGGCATTC CCACCAACTA CAAGGGGCAG
GTCCTGAGGC ACGTGAATGG CCAGGATCAG ATTGTGCCCG GCCTGTACGC CTGTGGGGAG
GCCGCCTGTG CCTCGGTACA TGGTGCCAAC CGCCTCGGGG CAAACTCGCT CTTGGACCTG
GTTGTCTTTG GTCGGGCATG TGCCCTGAGC ATCGAAGAGT CATGCAGGCC TGGAGATAAA
GTCCCTCCAA TTAAACCAAA CGCTGGGGAA GAATCTGTCA TGAATCTTGA CAAATTGAGA
TTTGCTGATG GAAGCATAAG AACATCGGAA CTGCGACTCA GCATGCAGAA GGTGCGGATT
GATGAGTACG ATTACTCCAA GCCCATCCAG GGGCAACAGA AGAAGCCCTT TGAGGAGCAC
TGGAGGAAGC ACACCCTGTC CTATGTGGAC GTTGGCACTG GGAAGTGCAC AGCCAGAGAA
CAACACATCA CACACAGGAA ACAGTTGTGC TCATGTGATG GGGGCCTCAG CACTAGGAAG
GAGCGGACTA TTGGTGCACG CAGCAGCTTG AATAAATCTG AAAGTCACTA TGCTGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu39348
Clone ID Related Accession (Same CDS sequence) XM_005248331
Accession Version XM_005248331.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1752bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_006576.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530378874. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGGGAGGAAT CAATGCTGCT CTGGGGAACA TGGAGGAGGA CAACTGGAGG
TGGCATTTCT ACGACACCGT GAAGGGCTCC GACTGGCTGG GGGACCAGGA TGCCATCCAC
TACATGACGG AGCAGGCCCC CGCCGCCGTG GTCGAGCTAG AAAATTATGG CATGCCGTTT
AGCAGAACTG AAGATGGGAA GATTTATCAG CGTGCATTTG GTGGACAGAG CCTCAAGTTT
GGAAAGGGCG GGCAGGCCCA TCGGTGCTGC TGTGTGGCTG ATCGGACTGG CCACTCGCTA
TTGCACACCT TATATGGAAG GTCTCTGCGA TATGATACCA GCTATTTTGT GGAGTATTTT
GCCTTGGATC TCCTGATGGA GAATGGGGAG TGCCGTGGTG TCATCGCACT GTGCATAGAG
GACGGGTCCA TCCATCGCAT AAGAGCAAAG AACACTGTTG TTGCCACAGG AGGCTACGGG
CGCACCTACT TCAGCTGCAC GTCTGCCCAC ACCAGCACTG GCGACGGCAC GGCCATGATC
ACCAGGGCAG GCCTTCCTTG CCAGGACCTA GAGTTTGTTC AGTTCCACCC TACAGGCATA
TATGGTGCTG GTTGTCTCAT TACGGAAGGA TGTCGTGGAG AGGGAGGCAT TCTCATTAAC
AGTCAAGGCG AAAGGTTTAT GGAGCGATAC GCCCCTGTCG CGAAGGACCT GGCGTCTAGA
GATGTGGTGT CTCGGTCCAT GACTCTGGAG ATCCGAGAAG GAAGAGGCTG TGGCCCTGAG
AAAGATCACG TCTACCTGCA GCTGCACCAC CTACCTCCAG AGCAGCTGGC CACGCGCCTG
CCTGGCATTT CAGAGACAGC CATGATCTTC GCTGGCGTGG ACGTCACGAA GGAGCCGATC
CCTGTCCTCC CCACCGTGCA TTATAACATG GGCGGCATTC CCACCAACTA CAAGGGGCAG
GTCCTGAGGC ACGTGAATGG CCAGGATCAG ATTGTGCCCG GCCTGTACGC CTGTGGGGAG
GCCGCCTGTG CCTCGGTACA TGGTGCCAAC CGCCTCGGGG CAAACTCGCT CTTGGACCTG
GTTGTCTTTG GTCGGGCATG TGCCCTGAGC ATCGAAGAGT CATGCAGGCC TGGAGATAAA
GTCCCTCCAA TTAAACCAAA CGCTGGGGAA GAATCTGTCA TGAATCTTGA CAAATTGAGA
TTTGCTGATG GAAGCATAAG AACATCGGAA CTGCGACTCA GCATGCAGAA GGTGCGGATT
GATGAGTACG ATTACTCCAA GCCCATCCAG GGGCAACAGA AGAAGCCCTT TGAGGAGCAC
TGGAGGAAGC ACACCCTGTC CTATGTGGAC GTTGGCACTG GGAAGGTCAC TCTGGAATAT
AGACCCGTGA TCGACAAAAC TTTGAACGAG GCTGACTGTG CCACCGTCCC GCCAGCCATT
CGCTCCTACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005248388.1
CDS134..1885
Misc Feature(1)251..1882(+)
Misc Feature(2)386..1546(+)
Misc Feature(3)1748..1882(+)
Translation

Target ORF information:

RefSeq Version XM_005248331
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (SDHA), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005248331

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGGGAGGAAT CAATGCTGCT CTGGGGAACA TGGAGGAGGA CAACTGGAGG
TGGCATTTCT ACGACACCGT GAAGGGCTCC GACTGGCTGG GGGACCAGGA TGCCATCCAC
TACATGACGG AGCAGGCCCC CGCCGCCGTG GTCGAGCTAG AAAATTATGG CATGCCGTTT
AGCAGAACTG AAGATGGGAA GATTTATCAG CGTGCATTTG GTGGACAGAG CCTCAAGTTT
GGAAAGGGCG GGCAGGCCCA TCGGTGCTGC TGTGTGGCTG ATCGGACTGG CCACTCGCTA
TTGCACACCT TATATGGAAG GTCTCTGCGA TATGATACCA GCTATTTTGT GGAGTATTTT
GCCTTGGATC TCCTGATGGA GAATGGGGAG TGCCGTGGTG TCATCGCACT GTGCATAGAG
GACGGGTCCA TCCATCGCAT AAGAGCAAAG AACACTGTTG TTGCCACAGG AGGCTACGGG
CGCACCTACT TCAGCTGCAC GTCTGCCCAC ACCAGCACTG GCGACGGCAC GGCCATGATC
ACCAGGGCAG GCCTTCCTTG CCAGGACCTA GAGTTTGTTC AGTTCCACCC TACAGGCATA
TATGGTGCTG GTTGTCTCAT TACGGAAGGA TGTCGTGGAG AGGGAGGCAT TCTCATTAAC
AGTCAAGGCG AAAGGTTTAT GGAGCGATAC GCCCCTGTCG CGAAGGACCT GGCGTCTAGA
GATGTGGTGT CTCGGTCCAT GACTCTGGAG ATCCGAGAAG GAAGAGGCTG TGGCCCTGAG
AAAGATCACG TCTACCTGCA GCTGCACCAC CTACCTCCAG AGCAGCTGGC CACGCGCCTG
CCTGGCATTT CAGAGACAGC CATGATCTTC GCTGGCGTGG ACGTCACGAA GGAGCCGATC
CCTGTCCTCC CCACCGTGCA TTATAACATG GGCGGCATTC CCACCAACTA CAAGGGGCAG
GTCCTGAGGC ACGTGAATGG CCAGGATCAG ATTGTGCCCG GCCTGTACGC CTGTGGGGAG
GCCGCCTGTG CCTCGGTACA TGGTGCCAAC CGCCTCGGGG CAAACTCGCT CTTGGACCTG
GTTGTCTTTG GTCGGGCATG TGCCCTGAGC ATCGAAGAGT CATGCAGGCC TGGAGATAAA
GTCCCTCCAA TTAAACCAAA CGCTGGGGAA GAATCTGTCA TGAATCTTGA CAAATTGAGA
TTTGCTGATG GAAGCATAAG AACATCGGAA CTGCGACTCA GCATGCAGAA GGTGCGGATT
GATGAGTACG ATTACTCCAA GCCCATCCAG GGGCAACAGA AGAAGCCCTT TGAGGAGCAC
TGGAGGAAGC ACACCCTGTC CTATGTGGAC GTTGGCACTG GGAAGGTCAC TCTGGAATAT
AGACCCGTGA TCGACAAAAC TTTGAACGAG GCTGACTGTG CCACCGTCCC GCCAGCCATT
CGCTCCTACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28692
Clone ID Related Accession (Same CDS sequence) NM_004168
Accession Version NM_004168.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1995bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA160919.1, BC001380.2, AK291311.1, AC021087.5 and HY265959.1. On Jul 1, 2014 this sequence version replaced gi:156416002. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK225027.1, BC001380.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGGGAGGAAT CAATGCTGCT CTGGGGAACA TGGAGGAGGA CAACTGGAGG
TGGCATTTCT ACGACACCGT GAAGGGCTCC GACTGGCTGG GGGACCAGGA TGCCATCCAC
TACATGACGG AGCAGGCCCC CGCCGCCGTG GTCGAGCTAG AAAATTATGG CATGCCGTTT
AGCAGAACTG AAGATGGGAA GATTTATCAG CGTGCATTTG GTGGACAGAG CCTCAAGTTT
GGAAAGGGCG GGCAGGCCCA TCGGTGCTGC TGTGTGGCTG ATCGGACTGG CCACTCGCTA
TTGCACACCT TATATGGAAG GTCTCTGCGA TATGATACCA GCTATTTTGT GGAGTATTTT
GCCTTGGATC TCCTGATGGA GAATGGGGAG TGCCGTGGTG TCATCGCACT GTGCATAGAG
GACGGGTCCA TCCATCGCAT AAGAGCAAAG AACACTGTTG TTGCCACAGG AGGCTACGGG
CGCACCTACT TCAGCTGCAC GTCTGCCCAC ACCAGCACTG GCGACGGCAC GGCCATGATC
ACCAGGGCAG GCCTTCCTTG CCAGGACCTA GAGTTTGTTC AGTTCCACCC TACAGGCATA
TATGGTGCTG GTTGTCTCAT TACGGAAGGA TGTCGTGGAG AGGGAGGCAT TCTCATTAAC
AGTCAAGGCG AAAGGTTTAT GGAGCGATAC GCCCCTGTCG CGAAGGACCT GGCGTCTAGA
GATGTGGTGT CTCGGTCCAT GACTCTGGAG ATCCGAGAAG GAAGAGGCTG TGGCCCTGAG
AAAGATCACG TCTACCTGCA GCTGCACCAC CTACCTCCAG AGCAGCTGGC CACGCGCCTG
CCTGGCATTT CAGAGACAGC CATGATCTTC GCTGGCGTGG ACGTCACGAA GGAGCCGATC
CCTGTCCTCC CCACCGTGCA TTATAACATG GGCGGCATTC CCACCAACTA CAAGGGGCAG
GTCCTGAGGC ACGTGAATGG CCAGGATCAG ATTGTGCCCG GCCTGTACGC CTGTGGGGAG
GCCGCCTGTG CCTCGGTACA TGGTGCCAAC CGCCTCGGGG CAAACTCGCT CTTGGACCTG
GTTGTCTTTG GTCGGGCATG TGCCCTGAGC ATCGAAGAGT CATGCAGGCC TGGAGATAAA
GTCCCTCCAA TTAAACCAAA CGCTGGGGAA GAATCTGTCA TGAATCTTGA CAAATTGAGA
TTTGCTGATG GAAGCATAAG AACATCGGAA CTGCGACTCA GCATGCAGAA GTCAATGCAA
AATCATGCTG CCGTGTTCCG TGTGGGAAGC GTGTTGCAAG AAGGTTGTGG GAAAATCAGC
AAGCTCTATG GAGACCTAAA GCACCTGAAG ACGTTCGACC GGGGAATGGT CTGGAACACG
GACCTGGTGG AGACCCTGGA GCTGCAGAAC CTGATGCTGT GTGCGCTGCA GACCATCTAC
GGAGCAGAGG CACGGAAGGA GTCACGGGGC GCGCATGCCA GGGAAGACTA CAAGGTGCGG
ATTGATGAGT ACGATTACTC CAAGCCCATC CAGGGGCAAC AGAAGAAGCC CTTTGAGGAG
CACTGGAGGA AGCACACCCT GTCCTATGTG GACGTTGGCA CTGGGAAGGT CACTCTGGAA
TATAGACCCG TGATCGACAA AACTTTGAAC GAGGCTGACT GTGCCACCGT CCCGCCAGCC
ATTCGCTCCT ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004159.2
CDS134..2128
Misc Feature(1)251..2125(+)
Misc Feature(2)386..1546(+)
Misc Feature(3)668..670(+)
Misc Feature(4)776..778(+)
Misc Feature(5)1136..1138(+)
Misc Feature(6)1667..2125(+)
Misc Feature(7)1754..1756(+)
Misc Feature(8)1955..1957(+)
Exon (1)1..196
Gene:SDHA
Gene Synonym:
Exon (2)197..283
Gene:SDHA
Gene Synonym:
Exon (3)284..445
Gene:SDHA
Gene Synonym:
Exon (4)446..589
Gene:SDHA
Gene Synonym:
Exon (5)590..754
Gene:SDHA
Gene Synonym:
Exon (6)755..903
Gene:SDHA
Gene Synonym:
Exon (7)904..1028
Gene:SDHA
Gene Synonym:
Exon (8)1029..1197
Gene:SDHA
Gene Synonym:
Exon (9)1198..1393
Gene:SDHA
Gene Synonym:
Exon (10)1394..1565
Gene:SDHA
Gene Synonym:
Exon (11)1566..1684
Gene:SDHA
Gene Synonym:
Exon (12)1685..1796
Gene:SDHA
Gene Synonym:
Exon (13)1797..1927
Gene:SDHA
Gene Synonym:
Exon (14)1928..2041
Gene:SDHA
Gene Synonym:
Exon (15)2042..2790
Gene:SDHA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_004168
Organism Homo sapiens (human)
Definition Homo sapiens succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (SDHA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004168

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGGGAGGAAT CAATGCTGCT CTGGGGAACA TGGAGGAGGA CAACTGGAGG
TGGCATTTCT ACGACACCGT GAAGGGCTCC GACTGGCTGG GGGACCAGGA TGCCATCCAC
TACATGACGG AGCAGGCCCC CGCCGCCGTG GTCGAGCTAG AAAATTATGG CATGCCGTTT
AGCAGAACTG AAGATGGGAA GATTTATCAG CGTGCATTTG GTGGACAGAG CCTCAAGTTT
GGAAAGGGCG GGCAGGCCCA TCGGTGCTGC TGTGTGGCTG ATCGGACTGG CCACTCGCTA
TTGCACACCT TATATGGAAG GTCTCTGCGA TATGATACCA GCTATTTTGT GGAGTATTTT
GCCTTGGATC TCCTGATGGA GAATGGGGAG TGCCGTGGTG TCATCGCACT GTGCATAGAG
GACGGGTCCA TCCATCGCAT AAGAGCAAAG AACACTGTTG TTGCCACAGG AGGCTACGGG
CGCACCTACT TCAGCTGCAC GTCTGCCCAC ACCAGCACTG GCGACGGCAC GGCCATGATC
ACCAGGGCAG GCCTTCCTTG CCAGGACCTA GAGTTTGTTC AGTTCCACCC TACAGGCATA
TATGGTGCTG GTTGTCTCAT TACGGAAGGA TGTCGTGGAG AGGGAGGCAT TCTCATTAAC
AGTCAAGGCG AAAGGTTTAT GGAGCGATAC GCCCCTGTCG CGAAGGACCT GGCGTCTAGA
GATGTGGTGT CTCGGTCCAT GACTCTGGAG ATCCGAGAAG GAAGAGGCTG TGGCCCTGAG
AAAGATCACG TCTACCTGCA GCTGCACCAC CTACCTCCAG AGCAGCTGGC CACGCGCCTG
CCTGGCATTT CAGAGACAGC CATGATCTTC GCTGGCGTGG ACGTCACGAA GGAGCCGATC
CCTGTCCTCC CCACCGTGCA TTATAACATG GGCGGCATTC CCACCAACTA CAAGGGGCAG
GTCCTGAGGC ACGTGAATGG CCAGGATCAG ATTGTGCCCG GCCTGTACGC CTGTGGGGAG
GCCGCCTGTG CCTCGGTACA TGGTGCCAAC CGCCTCGGGG CAAACTCGCT CTTGGACCTG
GTTGTCTTTG GTCGGGCATG TGCCCTGAGC ATCGAAGAGT CATGCAGGCC TGGAGATAAA
GTCCCTCCAA TTAAACCAAA CGCTGGGGAA GAATCTGTCA TGAATCTTGA CAAATTGAGA
TTTGCTGATG GAAGCATAAG AACATCGGAA CTGCGACTCA GCATGCAGAA GTCAATGCAA
AATCATGCTG CCGTGTTCCG TGTGGGAAGC GTGTTGCAAG AAGGTTGTGG GAAAATCAGC
AAGCTCTATG GAGACCTAAA GCACCTGAAG ACGTTCGACC GGGGAATGGT CTGGAACACG
GACCTGGTGG AGACCCTGGA GCTGCAGAAC CTGATGCTGT GTGCGCTGCA GACCATCTAC
GGAGCAGAGG CACGGAAGGA GTCACGGGGC GCGCATGCCA GGGAAGACTA CAAGGTGCGG
ATTGATGAGT ACGATTACTC CAAGCCCATC CAGGGGCAAC AGAAGAAGCC CTTTGAGGAG
CACTGGAGGA AGCACACCCT GTCCTATGTG GACGTTGGCA CTGGGAAGGT CACTCTGGAA
TATAGACCCG TGATCGACAA AACTTTGAAC GAGGCTGACT GTGCCACCGT CCCGCCAGCC
ATTCGCTCCT ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28693
Clone ID Related Accession (Same CDS sequence) NM_001294332
Accession Version NM_001294332.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1851bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA160919.1, AK295937.1, AK291311.1, AC021087.5 and HY265959.1. Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295937.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGCTAGAAAA TTATGGCATG CCGTTTAGCA GAACTGAAGA TGGGAAGATT
TATCAGCGTG CATTTGGTGG ACAGAGCCTC AAGTTTGGAA AGGGCGGGCA GGCCCATCGG
TGCTGCTGTG TGGCTGATCG GACTGGCCAC TCGCTATTGC ACACCTTATA TGGAAGGTCT
CTGCGATATG ATACCAGCTA TTTTGTGGAG TATTTTGCCT TGGATCTCCT GATGGAGAAT
GGGGAGTGCC GTGGTGTCAT CGCACTGTGC ATAGAGGACG GGTCCATCCA TCGCATAAGA
GCAAAGAACA CTGTTGTTGC CACAGGAGGC TACGGGCGCA CCTACTTCAG CTGCACGTCT
GCCCACACCA GCACTGGCGA CGGCACGGCC ATGATCACCA GGGCAGGCCT TCCTTGCCAG
GACCTAGAGT TTGTTCAGTT CCACCCTACA GGCATATATG GTGCTGGTTG TCTCATTACG
GAAGGATGTC GTGGAGAGGG AGGCATTCTC ATTAACAGTC AAGGCGAAAG GTTTATGGAG
CGATACGCCC CTGTCGCGAA GGACCTGGCG TCTAGAGATG TGGTGTCTCG GTCCATGACT
CTGGAGATCC GAGAAGGAAG AGGCTGTGGC CCTGAGAAAG ATCACGTCTA CCTGCAGCTG
CACCACCTAC CTCCAGAGCA GCTGGCCACG CGCCTGCCTG GCATTTCAGA GACAGCCATG
ATCTTCGCTG GCGTGGACGT CACGAAGGAG CCGATCCCTG TCCTCCCCAC CGTGCATTAT
AACATGGGCG GCATTCCCAC CAACTACAAG GGGCAGGTCC TGAGGCACGT GAATGGCCAG
GATCAGATTG TGCCCGGCCT GTACGCCTGT GGGGAGGCCG CCTGTGCCTC GGTACATGGT
GCCAACCGCC TCGGGGCAAA CTCGCTCTTG GACCTGGTTG TCTTTGGTCG GGCATGTGCC
CTGAGCATCG AAGAGTCATG CAGGCCTGGA GATAAAGTCC CTCCAATTAA ACCAAACGCT
GGGGAAGAAT CTGTCATGAA TCTTGACAAA TTGAGATTTG CTGATGGAAG CATAAGAACA
TCGGAACTGC GACTCAGCAT GCAGAAGTCA ATGCAAAATC ATGCTGCCGT GTTCCGTGTG
GGAAGCGTGT TGCAAGAAGG TTGTGGGAAA ATCAGCAAGC TCTATGGAGA CCTAAAGCAC
CTGAAGACGT TCGACCGGGG AATGGTCTGG AACACGGACC TGGTGGAGAC CCTGGAGCTG
CAGAACCTGA TGCTGTGTGC GCTGCAGACC ATCTACGGAG CAGAGGCACG GAAGGAGTCA
CGGGGCGCGC ATGCCAGGGA AGACTACAAG GTGCGGATTG ATGAGTACGA TTACTCCAAG
CCCATCCAGG GGCAACAGAA GAAGCCCTTT GAGGAGCACT GGAGGAAGCA CACCCTGTCC
TATGTGGACG TTGGCACTGG GAAGGTCACT CTGGAATATA GACCCGTGAT CGACAAAACT
TTGAACGAGG CTGACTGTGC CACCGTCCCG CCAGCCATTC GCTCCTACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001281261.1
CDS134..1984
Misc Feature(1)251..1981(+)
Misc Feature(2)443..1402(+)
Misc Feature(3)1523..1981(+)
Exon (1)1..196
Gene:SDHA
Gene Synonym:
Exon (2)197..283
Gene:SDHA
Gene Synonym:
Exon (3)284..445
Gene:SDHA
Gene Synonym:
Exon (4)446..610
Gene:SDHA
Gene Synonym:
Exon (5)611..759
Gene:SDHA
Gene Synonym:
Exon (6)760..884
Gene:SDHA
Gene Synonym:
Exon (7)885..1053
Gene:SDHA
Gene Synonym:
Exon (8)1054..1249
Gene:SDHA
Gene Synonym:
Exon (9)1250..1421
Gene:SDHA
Gene Synonym:
Exon (10)1422..1540
Gene:SDHA
Gene Synonym:
Exon (11)1541..1652
Gene:SDHA
Gene Synonym:
Exon (12)1653..1783
Gene:SDHA
Gene Synonym:
Exon (13)1784..1897
Gene:SDHA
Gene Synonym:
Exon (14)1898..2646
Gene:SDHA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001294332
Organism Homo sapiens (human)
Definition Homo sapiens succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (SDHA), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001294332

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGTCGGGGG TCCGGGGCCT GTCGCGGCTG CTGAGCGCTC GGCGCCTGGC GCTGGCCAAG 
GCGTGGCCAA CAGTGTTGCA AACAGGAACC CGAGGTTTTC ACTTCACTGT TGATGGGAAC
AAGAGGGCAT CTGCTAAAGT TTCAGATTCC ATTTCTGCTC AGTATCCAGT AGTGGATCAT
GAATTTGATG CAGTGGTGGT AGGCGCTGGA GGGGCAGGCT TGCGAGCTGC ATTTGGCCTT
TCTGAGGCAG GGTTTAATAC AGCATGTGTT ACCAAGCTGT TTCCTACCAG GTCACACACT
GTTGCAGCAC AGCTAGAAAA TTATGGCATG CCGTTTAGCA GAACTGAAGA TGGGAAGATT
TATCAGCGTG CATTTGGTGG ACAGAGCCTC AAGTTTGGAA AGGGCGGGCA GGCCCATCGG
TGCTGCTGTG TGGCTGATCG GACTGGCCAC TCGCTATTGC ACACCTTATA TGGAAGGTCT
CTGCGATATG ATACCAGCTA TTTTGTGGAG TATTTTGCCT TGGATCTCCT GATGGAGAAT
GGGGAGTGCC GTGGTGTCAT CGCACTGTGC ATAGAGGACG GGTCCATCCA TCGCATAAGA
GCAAAGAACA CTGTTGTTGC CACAGGAGGC TACGGGCGCA CCTACTTCAG CTGCACGTCT
GCCCACACCA GCACTGGCGA CGGCACGGCC ATGATCACCA GGGCAGGCCT TCCTTGCCAG
GACCTAGAGT TTGTTCAGTT CCACCCTACA GGCATATATG GTGCTGGTTG TCTCATTACG
GAAGGATGTC GTGGAGAGGG AGGCATTCTC ATTAACAGTC AAGGCGAAAG GTTTATGGAG
CGATACGCCC CTGTCGCGAA GGACCTGGCG TCTAGAGATG TGGTGTCTCG GTCCATGACT
CTGGAGATCC GAGAAGGAAG AGGCTGTGGC CCTGAGAAAG ATCACGTCTA CCTGCAGCTG
CACCACCTAC CTCCAGAGCA GCTGGCCACG CGCCTGCCTG GCATTTCAGA GACAGCCATG
ATCTTCGCTG GCGTGGACGT CACGAAGGAG CCGATCCCTG TCCTCCCCAC CGTGCATTAT
AACATGGGCG GCATTCCCAC CAACTACAAG GGGCAGGTCC TGAGGCACGT GAATGGCCAG
GATCAGATTG TGCCCGGCCT GTACGCCTGT GGGGAGGCCG CCTGTGCCTC GGTACATGGT
GCCAACCGCC TCGGGGCAAA CTCGCTCTTG GACCTGGTTG TCTTTGGTCG GGCATGTGCC
CTGAGCATCG AAGAGTCATG CAGGCCTGGA GATAAAGTCC CTCCAATTAA ACCAAACGCT
GGGGAAGAAT CTGTCATGAA TCTTGACAAA TTGAGATTTG CTGATGGAAG CATAAGAACA
TCGGAACTGC GACTCAGCAT GCAGAAGTCA ATGCAAAATC ATGCTGCCGT GTTCCGTGTG
GGAAGCGTGT TGCAAGAAGG TTGTGGGAAA ATCAGCAAGC TCTATGGAGA CCTAAAGCAC
CTGAAGACGT TCGACCGGGG AATGGTCTGG AACACGGACC TGGTGGAGAC CCTGGAGCTG
CAGAACCTGA TGCTGTGTGC GCTGCAGACC ATCTACGGAG CAGAGGCACG GAAGGAGTCA
CGGGGCGCGC ATGCCAGGGA AGACTACAAG GTGCGGATTG ATGAGTACGA TTACTCCAAG
CCCATCCAGG GGCAACAGAA GAAGCCCTTT GAGGAGCACT GGAGGAAGCA CACCCTGTCC
TATGTGGACG TTGGCACTGG GAAGGTCACT CTGGAATATA GACCCGTGAT CGACAAAACT
TTGAACGAGG CTGACTGTGC CACCGTCCCG CCAGCCATTC GCTCCTACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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