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BLM cDNA ORF clone, Homo sapiens (human)

Gene Symbol BLM
Entrez Gene ID 641
Full Name Bloom syndrome, RecQ helicase-like
Synonyms BS, RECQ2, RECQL2, RECQL3
General protein information
Preferred Names
Bloom syndrome protein
Names
Bloom syndrome protein
recQ protein-like 3
DNA helicase, RecQ-like type 2
NP_000048.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

15

15q26.1

Summary The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

604610

Disorder Html: Bloom syndrome, 210900 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011521881 XP_011520183 Bloom syndrome protein isoform X1
XM_011521882 XP_011520184 Bloom syndrome protein isoform X2
XM_006720632 XP_006720695 Bloom syndrome protein isoform X3
NM_000057 NP_000048 Bloom syndrome protein isoform 1
NM_001287246 NP_001274175 Bloom syndrome protein isoform 1
NM_001287247 NP_001274176 Bloom syndrome protein isoform 2
NM_001287248 NP_001274177 Bloom syndrome protein isoform 3



Homo sapiens (human) BLM NP_000048.1
Pan troglodytes (chimpanzee) BLM XP_510594.2
Macaca mulatta (Rhesus monkey) BLM XP_001097543.1
Canis lupus familiaris (dog) BLM XP_003434427.1
Bos taurus (cattle) BLM XP_002696629.1
Mus musculus (house mouse) Blm NP_031576.4
Rattus norvegicus (Norway rat) Blm XP_006229520.1
Gallus gallus (chicken) BLM NP_001007088.2
Danio rerio (zebrafish) blm XP_701357.4
Xenopus (Silurana) tropicalis (western clawed frog) blm NP_001243837.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BLM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BLM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu56490 XM_011521881 PREDICTED: Homo sapiens Bloom syndrome, RecQ helicase-like (BLM), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $475.30
$679.00
OHu56491 XM_011521882 PREDICTED: Homo sapiens Bloom syndrome, RecQ helicase-like (BLM), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu48483 XM_006720632 PREDICTED: Homo sapiens Bloom syndrome, RecQ helicase-like (BLM), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00
OHu20445 NM_000057 Homo sapiens Bloom syndrome, RecQ helicase-like (BLM), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 16 $727.30
$1039.00
OHu20445 NM_001287246 Homo sapiens Bloom syndrome, RecQ helicase-like (BLM), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 16 $727.30
$1039.00
OHu20391 NM_001287247 Homo sapiens Bloom syndrome, RecQ helicase-like (BLM), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu20947 NM_001287248 Homo sapiens Bloom syndrome, RecQ helicase-like (BLM), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 16 $559.30
$799.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu56490
Clone ID Related Accession (Same CDS sequence) XM_011521881
Accession Version XM_011521881.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2940bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product Bloom syndrome protein isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGGAGGGTG ATTCCTGCCC TACAGGGAAT TCTATGAAGG AGTTAAATTT TTCACACCTT 
CCCTCAAATT CTGTTTCTCC TGGGGACTGT TTACTGACTA CCACCCTAGG AAAGACAGGA
TTCTCTGCCA CCAGGAAGAA TCTTTTTGAA AGGCCTTTAT TCAATACCCA TTTACAGAAG
TCCTTTGTAA GTAGCAACTG GGCTGAAACA CCAAGACTAG GAAAAAAAAA TGAAAGCTCT
TATTTCCCAG GAAATGTTCT CACAAGCACT GCTGTGAAAG ATCAGAATAA ACATACTGCT
TCAATAAATG ACTTAGAAAG AGAAACCCAA CCTTCCTATG ATATTGATAA TTTTGACATA
GATGACTTTG ATGATGATGA TGACTGGGAA GACATAATGC ATAATTTAGC AGCCAGCAAA
TCTTCCACAG CTGCCTATCA ACCCATCAAG GAAGGTCGGC CAATTAAATC AGTATCAGAA
AGACTTTCCT CAGCCAAGAC AGACTGTCTT CCAGTGTCAT CTACTGCTCA AAATATAAAC
TTCTCAGAGT CAATTCAGAA TTATACTGAC AAGTCAGCAC AAAATTTAGC ATCCAGAAAT
CTGAAACATG AGCGTTTCCA AAGTCTTAGT TTTCCTCATA CAAAGGAAAT GATGAAGATT
TTTCATAAAA AATTTGGCCT GCATAATTTT AGAACTAATC AGCTAGAGGC GATCAATGCT
GCACTGCTTG GTGAAGACTG TTTTATCCTG ATGCCGACTG GAGGTGGTAA GAGTTTGTGT
TACCAGCTCC CTGCCTGTGT TTCTCCTGGG GTCACTGTTG TCATTTCTCC CTTGAGATCA
CTTATCGTAG ATCAAGTCCA AAAGCTGACT TCCTTGGATA TTCCAGCTAC ATATCTGACA
GGTGATAAGA CTGACTCAGA AGCTACAAAT ATTTACCTCC AGTTATCAAA AAAAGACCCA
ATCATAAAAC TTCTATATGT CACTCCAGAA AAGATCTGTG CAAGTAACAG ACTCATTTCT
ACTCTGGAGA ATCTCTATGA GAGGAAGCTC TTGGCACGTT TTGTTATTGA TGAAGCACAT
TGTGTCAGTC AGTGGGGACA TGATTTTCGT CAAGATTACA AAAGAATGAA TATGCTTCGC
CAGAAGTTTC CTTCTGTTCC GGTGATGGCT CTTACGGCCA CAGCTAATCC CAGGGTACAG
AAGGACATCC TGACTCAGCT GAAGATTCTC AGACCTCAGG TGTTTAGCAT GAGCTTTAAC
AGACATAATC TGAAATACTA TGTATTACCG AAAAAGCCTA AAAAGGTGGC ATTTGATTGC
CTAGAATGGA TCAGAAAGCA CCACCCATAT GATTCAGGGA TAATTTACTG CCTCTCCAGG
CGAGAATGTG ACACCATGGC TGACACGTTA CAGAGAGATG GGCTCGCTGC TCTTGCTTAC
CATGCTGGCC TCAGTGATTC TGCCAGAGAT GAAGTGCAGC AGAAGTGGAT TAATCAGGAT
GGCTGTCAGG TTATCTGTGC TACAATTGCA TTTGGAATGG GGATTGACAA ACCGGACGTG
CGATTTGTGA TTCATGCATC TCTCCCTAAA TCTGTGGAGG GTTACTACCA AGAATCTGGC
AGAGCTGGAA GAGATGGGGA AATATCTCAC TGCCTGCTTT TCTATACCTA TCATGATGTG
ACCAGACTGA AAAGACTTAT AATGATGGAA AAAGATGGAA ACCATCATAC AAGAGAAACT
CACTTCAATA ATTTGTATAG CATGGTACAT TACTGTGAAA ATATAACGGA ATGCAGGAGA
ATACAGCTTT TGGCCTACTT TGGTGAAAAT GGATTTAATC CTGATTTTTG TAAGAAACAC
CCAGATGTTT CTTGTGATAA TTGCTGTAAA ACAAAGGATT ATAAAACAAG AGATGTGACT
GACGATGTGA AAAGTATTGT AAGATTTGTT CAAGAACATA GTTCATCACA AGGAATGAGA
AATATAAAAC ATGTAGGTCC TTCTGGAAGA TTTACTATGA ATATGCTGGT CGACATTTTC
TTGGGGAGTA AGAGTGCAAA AATCCAGTCA GGTATATTTG GAAAAGGATC TGCTTATTCA
CGACACAATG CCGAAAGACT TTTTAAAAAG CTGATACTTG ACAAGATTTT GGATGAAGAC
TTATATATCA ATGCCAATGA CCAGGCGATC GCTTATGTGA TGCTCGGAAA TAAAGCCCAA
ACTGTACTAA ATGGCAATTT AAAGGTAGAC TTTATGGAAA CAGAAAATTC CAGCAGTGTG
AAAAAACAAA AAGCGTTAGT AGCAAAAGTG TCTCAGAGGG AAGAGATGGT TAAAAAATGT
CTTGGAGAAC TTACAGAAGT CTGCAAATCT CTGGGGAAAG TTTTTGGTGT CCATTACTTC
AATATTTTTA ATACCGTCAC TCTCAAGAAG CTTGCAGAAT CTTTATCTTC TGATCCTGAG
GTTTTGCTTC AAATTGATGG TGTTACTGAA GACAAACTGG AAAAATATGG TGCGGAAGTG
ATTTCAGTAT TACAGAAATA CTCTGAATGG ACATCGCCAG CTGAAGACAG TTCCCCAGGG
ATAAGCCTGT CCAGCAGCAG AGGCCCCGGA AGAAGTGCCG CTGAGGAGCT CGACGAGGAA
ATACCCGTAT CTTCCCACTA CTTTGCAAGT AAAACCAGAA ATGAAAGGAA GAGGAAAAAG
ATGCCAGCCT CCCAAAGGTC TAAGAGGAGA AAAACTGCTT CCAGTGGTTC CAAGGCAAAG
GGGGGGTCTG CCACATGTAG AAAGATATCT TCCAAAACGA AATCCTCCAG CATCATTGGA
TCCAGTTCAG CCTCACATAC TTCTCAAGCG ACATCAGGAG CCAATAGCAA ATTGGGGATT
ATGGCTCCAC CGAAGCCTAT AAATAGACCG TTTCTTAAGC CTTCATATGC ATTCTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011520183.1
CDS443..3382
Misc Feature(1)1097..2557(+)
Misc Feature(2)1175..1624(+)
Misc Feature(3)1202..1216(+)
Misc Feature(4)1511..1522(+)
Misc Feature(5)1709..2104(+)
Misc Feature(6)1814..1972(+)
Misc Feature(7)1988..2074(+)
Misc Feature(8)2354..2671(+)
Misc Feature(9)2765..3004(+)
Translation

Target ORF information:

RefSeq Version XM_011521881
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Bloom syndrome, RecQ helicase-like (BLM), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011521881

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGGAGGGTG ATTCCTGCCC TACAGGGAAT TCTATGAAGG AGTTAAATTT TTCACACCTT 
CCCTCAAATT CTGTTTCTCC TGGGGACTGT TTACTGACTA CCACCCTAGG AAAGACAGGA
TTCTCTGCCA CCAGGAAGAA TCTTTTTGAA AGGCCTTTAT TCAATACCCA TTTACAGAAG
TCCTTTGTAA GTAGCAACTG GGCTGAAACA CCAAGACTAG GAAAAAAAAA TGAAAGCTCT
TATTTCCCAG GAAATGTTCT CACAAGCACT GCTGTGAAAG ATCAGAATAA ACATACTGCT
TCAATAAATG ACTTAGAAAG AGAAACCCAA CCTTCCTATG ATATTGATAA TTTTGACATA
GATGACTTTG ATGATGATGA TGACTGGGAA GACATAATGC ATAATTTAGC AGCCAGCAAA
TCTTCCACAG CTGCCTATCA ACCCATCAAG GAAGGTCGGC CAATTAAATC AGTATCAGAA
AGACTTTCCT CAGCCAAGAC AGACTGTCTT CCAGTGTCAT CTACTGCTCA AAATATAAAC
TTCTCAGAGT CAATTCAGAA TTATACTGAC AAGTCAGCAC AAAATTTAGC ATCCAGAAAT
CTGAAACATG AGCGTTTCCA AAGTCTTAGT TTTCCTCATA CAAAGGAAAT GATGAAGATT
TTTCATAAAA AATTTGGCCT GCATAATTTT AGAACTAATC AGCTAGAGGC GATCAATGCT
GCACTGCTTG GTGAAGACTG TTTTATCCTG ATGCCGACTG GAGGTGGTAA GAGTTTGTGT
TACCAGCTCC CTGCCTGTGT TTCTCCTGGG GTCACTGTTG TCATTTCTCC CTTGAGATCA
CTTATCGTAG ATCAAGTCCA AAAGCTGACT TCCTTGGATA TTCCAGCTAC ATATCTGACA
GGTGATAAGA CTGACTCAGA AGCTACAAAT ATTTACCTCC AGTTATCAAA AAAAGACCCA
ATCATAAAAC TTCTATATGT CACTCCAGAA AAGATCTGTG CAAGTAACAG ACTCATTTCT
ACTCTGGAGA ATCTCTATGA GAGGAAGCTC TTGGCACGTT TTGTTATTGA TGAAGCACAT
TGTGTCAGTC AGTGGGGACA TGATTTTCGT CAAGATTACA AAAGAATGAA TATGCTTCGC
CAGAAGTTTC CTTCTGTTCC GGTGATGGCT CTTACGGCCA CAGCTAATCC CAGGGTACAG
AAGGACATCC TGACTCAGCT GAAGATTCTC AGACCTCAGG TGTTTAGCAT GAGCTTTAAC
AGACATAATC TGAAATACTA TGTATTACCG AAAAAGCCTA AAAAGGTGGC ATTTGATTGC
CTAGAATGGA TCAGAAAGCA CCACCCATAT GATTCAGGGA TAATTTACTG CCTCTCCAGG
CGAGAATGTG ACACCATGGC TGACACGTTA CAGAGAGATG GGCTCGCTGC TCTTGCTTAC
CATGCTGGCC TCAGTGATTC TGCCAGAGAT GAAGTGCAGC AGAAGTGGAT TAATCAGGAT
GGCTGTCAGG TTATCTGTGC TACAATTGCA TTTGGAATGG GGATTGACAA ACCGGACGTG
CGATTTGTGA TTCATGCATC TCTCCCTAAA TCTGTGGAGG GTTACTACCA AGAATCTGGC
AGAGCTGGAA GAGATGGGGA AATATCTCAC TGCCTGCTTT TCTATACCTA TCATGATGTG
ACCAGACTGA AAAGACTTAT AATGATGGAA AAAGATGGAA ACCATCATAC AAGAGAAACT
CACTTCAATA ATTTGTATAG CATGGTACAT TACTGTGAAA ATATAACGGA ATGCAGGAGA
ATACAGCTTT TGGCCTACTT TGGTGAAAAT GGATTTAATC CTGATTTTTG TAAGAAACAC
CCAGATGTTT CTTGTGATAA TTGCTGTAAA ACAAAGGATT ATAAAACAAG AGATGTGACT
GACGATGTGA AAAGTATTGT AAGATTTGTT CAAGAACATA GTTCATCACA AGGAATGAGA
AATATAAAAC ATGTAGGTCC TTCTGGAAGA TTTACTATGA ATATGCTGGT CGACATTTTC
TTGGGGAGTA AGAGTGCAAA AATCCAGTCA GGTATATTTG GAAAAGGATC TGCTTATTCA
CGACACAATG CCGAAAGACT TTTTAAAAAG CTGATACTTG ACAAGATTTT GGATGAAGAC
TTATATATCA ATGCCAATGA CCAGGCGATC GCTTATGTGA TGCTCGGAAA TAAAGCCCAA
ACTGTACTAA ATGGCAATTT AAAGGTAGAC TTTATGGAAA CAGAAAATTC CAGCAGTGTG
AAAAAACAAA AAGCGTTAGT AGCAAAAGTG TCTCAGAGGG AAGAGATGGT TAAAAAATGT
CTTGGAGAAC TTACAGAAGT CTGCAAATCT CTGGGGAAAG TTTTTGGTGT CCATTACTTC
AATATTTTTA ATACCGTCAC TCTCAAGAAG CTTGCAGAAT CTTTATCTTC TGATCCTGAG
GTTTTGCTTC AAATTGATGG TGTTACTGAA GACAAACTGG AAAAATATGG TGCGGAAGTG
ATTTCAGTAT TACAGAAATA CTCTGAATGG ACATCGCCAG CTGAAGACAG TTCCCCAGGG
ATAAGCCTGT CCAGCAGCAG AGGCCCCGGA AGAAGTGCCG CTGAGGAGCT CGACGAGGAA
ATACCCGTAT CTTCCCACTA CTTTGCAAGT AAAACCAGAA ATGAAAGGAA GAGGAAAAAG
ATGCCAGCCT CCCAAAGGTC TAAGAGGAGA AAAACTGCTT CCAGTGGTTC CAAGGCAAAG
GGGGGGTCTG CCACATGTAG AAAGATATCT TCCAAAACGA AATCCTCCAG CATCATTGGA
TCCAGTTCAG CCTCACATAC TTCTCAAGCG ACATCAGGAG CCAATAGCAA ATTGGGGATT
ATGGCTCCAC CGAAGCCTAT AAATAGACCG TTTCTTAAGC CTTCATATGC ATTCTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56491
Clone ID Related Accession (Same CDS sequence) XM_011521882
Accession Version XM_011521882.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2724bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product Bloom syndrome protein isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTAATTG TACAACCATC CCCAATATCT
AAGTTTACAG CATTTTTATC ACCCCAGCGA GAAACCTGGT ACCCATTAGC AGCTGCTCCG
CATCTTCTCC CTCTTGTTTG TGCCCGGCTT CTTTCACTGA GCGTAGTTTT CAAGGGTCAT
CCATGTTGTA GCATTGGTCA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011520184.1
CDS107..2830
Misc Feature(1)674..928(+)
Misc Feature(2)1217..1339(+)
Misc Feature(3)2153..2602(+)
Misc Feature(4)2180..2194(+)
Misc Feature(5)2489..2500(+)
Translation

Target ORF information:

RefSeq Version XM_011521882
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Bloom syndrome, RecQ helicase-like (BLM), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011521882

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTAATTG TACAACCATC CCCAATATCT
AAGTTTACAG CATTTTTATC ACCCCAGCGA GAAACCTGGT ACCCATTAGC AGCTGCTCCG
CATCTTCTCC CTCTTGTTTG TGCCCGGCTT CTTTCACTGA GCGTAGTTTT CAAGGGTCAT
CCATGTTGTA GCATTGGTCA GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48483
Clone ID Related Accession (Same CDS sequence) XM_006720632
Accession Version XM_006720632.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2292bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product Bloom syndrome protein isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578827269. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGATGAAGA TTTTTCATAA AAAATTTGGC CTGCATAATT TTAGAACTAA TCAGCTAGAG 
GCGATCAATG CTGCACTGCT TGGTGAAGAC TGTTTTATCC TGATGCCGAC TGGAGGTGGT
AAGAGTTTGT GTTACCAGCT CCCTGCCTGT GTTTCTCCTG GGGTCACTGT TGTCATTTCT
CCCTTGAGAT CACTTATCGT AGATCAAGTC CAAAAGCTGA CTTCCTTGGA TATTCCAGCT
ACATATCTGA CAGGTGATAA GACTGACTCA GAAGCTACAA ATATTTACCT CCAGTTATCA
AAAAAAGACC CAATCATAAA ACTTCTATAT GTCACTCCAG AAAAGATCTG TGCAAGTAAC
AGACTCATTT CTACTCTGGA GAATCTCTAT GAGAGGAAGC TCTTGGCACG TTTTGTTATT
GATGAAGCAC ATTGTGTCAG TCAGTGGGGA CATGATTTTC GTCAAGATTA CAAAAGAATG
AATATGCTTC GCCAGAAGTT TCCTTCTGTT CCGGTGATGG CTCTTACGGC CACAGCTAAT
CCCAGGGTAC AGAAGGACAT CCTGACTCAG CTGAAGATTC TCAGACCTCA GGTGTTTAGC
ATGAGCTTTA ACAGACATAA TCTGAAATAC TATGTATTAC CGAAAAAGCC TAAAAAGGTG
GCATTTGATT GCCTAGAATG GATCAGAAAG CACCACCCAT ATGATTCAGG GATAATTTAC
TGCCTCTCCA GGCGAGAATG TGACACCATG GCTGACACGT TACAGAGAGA TGGGCTCGCT
GCTCTTGCTT ACCATGCTGG CCTCAGTGAT TCTGCCAGAG ATGAAGTGCA GCAGAAGTGG
ATTAATCAGG ATGGCTGTCA GGTTATCTGT GCTACAATTG CATTTGGAAT GGGGATTGAC
AAACCGGACG TGCGATTTGT GATTCATGCA TCTCTCCCTA AATCTGTGGA GGGTTACTAC
CAAGAATCTG GCAGAGCTGG AAGAGATGGG GAAATATCTC ACTGCCTGCT TTTCTATACC
TATCATGATG TGACCAGACT GAAAAGACTT ATAATGATGG AAAAAGATGG AAACCATCAT
ACAAGAGAAA CTCACTTCAA TAATTTGTAT AGCATGGTAC ATTACTGTGA AAATATAACG
GAATGCAGGA GAATACAGCT TTTGGCCTAC TTTGGTGAAA ATGGATTTAA TCCTGATTTT
TGTAAGAAAC ACCCAGATGT TTCTTGTGAT AATTGCTGTA AAACAAAGGA TTATAAAACA
AGAGATGTGA CTGACGATGT GAAAAGTATT GTAAGATTTG TTCAAGAACA TAGTTCATCA
CAAGGAATGA GAAATATAAA ACATGTAGGT CCTTCTGGAA GATTTACTAT GAATATGCTG
GTCGACATTT TCTTGGGGAG TAAGAGTGCA AAAATCCAGT CAGGTATATT TGGAAAAGGA
TCTGCTTATT CACGACACAA TGCCGAAAGA CTTTTTAAAA AGCTGATACT TGACAAGATT
TTGGATGAAG ACTTATATAT CAATGCCAAT GACCAGGCGA TCGCTTATGT GATGCTCGGA
AATAAAGCCC AAACTGTACT AAATGGCAAT TTAAAGGTAG ACTTTATGGA AACAGAAAAT
TCCAGCAGTG TGAAAAAACA AAAAGCGTTA GTAGCAAAAG TGTCTCAGAG GGAAGAGATG
GTTAAAAAAT GTCTTGGAGA ACTTACAGAA GTCTGCAAAT CTCTGGGGAA AGTTTTTGGT
GTCCATTACT TCAATATTTT TAATACCGTC ACTCTCAAGA AGCTTGCAGA ATCTTTATCT
TCTGATCCTG AGGTTTTGCT TCAAATTGAT GGTGTTACTG AAGACAAACT GGAAAAATAT
GGTGCGGAAG TGATTTCAGT ATTACAGAAA TACTCTGAAT GGACATCGCC AGCTGAAGAC
AGTTCCCCAG GGATAAGCCT GTCCAGCAGC AGAGGCCCCG GAAGAAGTGC CGCTGAGGAG
CTCGACGAGG AAATACCCGT ATCTTCCCAC TACTTTGCAA GTAAAACCAG AAATGAAAGG
AAGAGGAAAA AGATGCCAGC CTCCCAAAGG TCTAAGAGGA GAAAAACTGC TTCCAGTGGT
TCCAAGGCAA AGGGGGGGTC TGCCACATGT AGAAAGATAT CTTCCAAAAC GAAATCCTCC
AGCATCATTG GATCCAGTTC AGCCTCACAT ACTTCTCAAG CGACATCAGG AGCCAATAGC
AAATTGGGGA TTATGGCTCC ACCGAAGCCT ATAAATAGAC CGTTTCTTAA GCCTTCATAT
GCATTCTCAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006720695.1
CDS140..2431
Misc Feature(1)146..1606(+)
Misc Feature(2)224..673(+)
Misc Feature(3)251..265(+)
Misc Feature(4)560..571(+)
Misc Feature(5)758..1153(+)
Misc Feature(6)863..1021(+)
Misc Feature(7)1037..1123(+)
Misc Feature(8)1403..1720(+)
Misc Feature(9)1814..2053(+)
Translation

Target ORF information:

RefSeq Version XM_006720632
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Bloom syndrome, RecQ helicase-like (BLM), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006720632

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGATGAAGA TTTTTCATAA AAAATTTGGC CTGCATAATT TTAGAACTAA TCAGCTAGAG 
GCGATCAATG CTGCACTGCT TGGTGAAGAC TGTTTTATCC TGATGCCGAC TGGAGGTGGT
AAGAGTTTGT GTTACCAGCT CCCTGCCTGT GTTTCTCCTG GGGTCACTGT TGTCATTTCT
CCCTTGAGAT CACTTATCGT AGATCAAGTC CAAAAGCTGA CTTCCTTGGA TATTCCAGCT
ACATATCTGA CAGGTGATAA GACTGACTCA GAAGCTACAA ATATTTACCT CCAGTTATCA
AAAAAAGACC CAATCATAAA ACTTCTATAT GTCACTCCAG AAAAGATCTG TGCAAGTAAC
AGACTCATTT CTACTCTGGA GAATCTCTAT GAGAGGAAGC TCTTGGCACG TTTTGTTATT
GATGAAGCAC ATTGTGTCAG TCAGTGGGGA CATGATTTTC GTCAAGATTA CAAAAGAATG
AATATGCTTC GCCAGAAGTT TCCTTCTGTT CCGGTGATGG CTCTTACGGC CACAGCTAAT
CCCAGGGTAC AGAAGGACAT CCTGACTCAG CTGAAGATTC TCAGACCTCA GGTGTTTAGC
ATGAGCTTTA ACAGACATAA TCTGAAATAC TATGTATTAC CGAAAAAGCC TAAAAAGGTG
GCATTTGATT GCCTAGAATG GATCAGAAAG CACCACCCAT ATGATTCAGG GATAATTTAC
TGCCTCTCCA GGCGAGAATG TGACACCATG GCTGACACGT TACAGAGAGA TGGGCTCGCT
GCTCTTGCTT ACCATGCTGG CCTCAGTGAT TCTGCCAGAG ATGAAGTGCA GCAGAAGTGG
ATTAATCAGG ATGGCTGTCA GGTTATCTGT GCTACAATTG CATTTGGAAT GGGGATTGAC
AAACCGGACG TGCGATTTGT GATTCATGCA TCTCTCCCTA AATCTGTGGA GGGTTACTAC
CAAGAATCTG GCAGAGCTGG AAGAGATGGG GAAATATCTC ACTGCCTGCT TTTCTATACC
TATCATGATG TGACCAGACT GAAAAGACTT ATAATGATGG AAAAAGATGG AAACCATCAT
ACAAGAGAAA CTCACTTCAA TAATTTGTAT AGCATGGTAC ATTACTGTGA AAATATAACG
GAATGCAGGA GAATACAGCT TTTGGCCTAC TTTGGTGAAA ATGGATTTAA TCCTGATTTT
TGTAAGAAAC ACCCAGATGT TTCTTGTGAT AATTGCTGTA AAACAAAGGA TTATAAAACA
AGAGATGTGA CTGACGATGT GAAAAGTATT GTAAGATTTG TTCAAGAACA TAGTTCATCA
CAAGGAATGA GAAATATAAA ACATGTAGGT CCTTCTGGAA GATTTACTAT GAATATGCTG
GTCGACATTT TCTTGGGGAG TAAGAGTGCA AAAATCCAGT CAGGTATATT TGGAAAAGGA
TCTGCTTATT CACGACACAA TGCCGAAAGA CTTTTTAAAA AGCTGATACT TGACAAGATT
TTGGATGAAG ACTTATATAT CAATGCCAAT GACCAGGCGA TCGCTTATGT GATGCTCGGA
AATAAAGCCC AAACTGTACT AAATGGCAAT TTAAAGGTAG ACTTTATGGA AACAGAAAAT
TCCAGCAGTG TGAAAAAACA AAAAGCGTTA GTAGCAAAAG TGTCTCAGAG GGAAGAGATG
GTTAAAAAAT GTCTTGGAGA ACTTACAGAA GTCTGCAAAT CTCTGGGGAA AGTTTTTGGT
GTCCATTACT TCAATATTTT TAATACCGTC ACTCTCAAGA AGCTTGCAGA ATCTTTATCT
TCTGATCCTG AGGTTTTGCT TCAAATTGAT GGTGTTACTG AAGACAAACT GGAAAAATAT
GGTGCGGAAG TGATTTCAGT ATTACAGAAA TACTCTGAAT GGACATCGCC AGCTGAAGAC
AGTTCCCCAG GGATAAGCCT GTCCAGCAGC AGAGGCCCCG GAAGAAGTGC CGCTGAGGAG
CTCGACGAGG AAATACCCGT ATCTTCCCAC TACTTTGCAA GTAAAACCAG AAATGAAAGG
AAGAGGAAAA AGATGCCAGC CTCCCAAAGG TCTAAGAGGA GAAAAACTGC TTCCAGTGGT
TCCAAGGCAA AGGGGGGGTC TGCCACATGT AGAAAGATAT CTTCCAAAAC GAAATCCTCC
AGCATCATTG GATCCAGTTC AGCCTCACAT ACTTCTCAAG CGACATCAGG AGCCAATAGC
AAATTGGGGA TTATGGCTCC ACCGAAGCCT ATAAATAGAC CGTTTCTTAA GCCTTCATAT
GCATTCTCAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20445
Clone ID Related Accession (Same CDS sequence) NM_000057 , NM_001287246
Accession Version NM_000057.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4254bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product Bloom syndrome protein isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD707743.1, BC062697.1, U39817.1, BC115030.1, BC034480.1 and AC124248.6. On Dec 11, 2013 this sequence version replaced gi:105990533. Transcript Variant: This variant (1) encodes the longest protein (isoform 1). Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC034480.1, U39817.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT
AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA
TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA
TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT
GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT
CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT
GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT
GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA
CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC
AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG
CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT
GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT
GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA
AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGGG
AGTAAGAGTG CAAAAATCCA GTCAGGTATA TTTGGAAAAG GATCTGCTTA TTCACGACAC
AATGCCGAAA GACTTTTTAA AAAGCTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT
ATCAATGCCA ATGACCAGGC GATCGCTTAT GTGATGCTCG GAAATAAAGC CCAAACTGTA
CTAAATGGCA ATTTAAAGGT AGACTTTATG GAAACAGAAA ATTCCAGCAG TGTGAAAAAA
CAAAAAGCGT TAGTAGCAAA AGTGTCTCAG AGGGAAGAGA TGGTTAAAAA ATGTCTTGGA
GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GTGTCCATTA CTTCAATATT
TTTAATACCG TCACTCTCAA GAAGCTTGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG
CTTCAAATTG ATGGTGTTAC TGAAGACAAA CTGGAAAAAT ATGGTGCGGA AGTGATTTCA
GTATTACAGA AATACTCTGA ATGGACATCG CCAGCTGAAG ACAGTTCCCC AGGGATAAGC
CTGTCCAGCA GCAGAGGCCC CGGAAGAAGT GCCGCTGAGG AGCTCGACGA GGAAATACCC
GTATCTTCCC ACTACTTTGC AAGTAAAACC AGAAATGAAA GGAAGAGGAA AAAGATGCCA
GCCTCCCAAA GGTCTAAGAG GAGAAAAACT GCTTCCAGTG GTTCCAAGGC AAAGGGGGGG
TCTGCCACAT GTAGAAAGAT ATCTTCCAAA ACGAAATCCT CCAGCATCAT TGGATCCAGT
TCAGCCTCAC ATACTTCTCA AGCGACATCA GGAGCCAATA GCAAATTGGG GATTATGGCT
CCACCGAAGC CTATAAATAG ACCGTTTCTT AAGCCTTCAT ATGCATTCTC ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000048.1
CDS119..4372
Misc Feature(1)200..202(+)
Misc Feature(2)260..262(+)
Misc Feature(3)287..289(+)
Misc Feature(4)458..460(+)
Misc Feature(5)620..622(+)
Misc Feature(6)629..631(+)
Misc Feature(7)686..940(+)
Misc Feature(8)1019..1918(+)
Misc Feature(9)1190..1192(+)
Misc Feature(10)1229..1351(+)
Misc Feature(11)1373..1375(+)
Misc Feature(12)1373..1375(+)
Misc Feature(13)1382..1384(+)
Misc Feature(14)1382..1384(+)
Misc Feature(15)1394..1396(+)
Misc Feature(16)1424..1426(+)
Misc Feature(17)1613..1615(+)
Misc Feature(18)1640..1642(+)
Misc Feature(19)2087..3547(+)
Misc Feature(20)2165..2614(+)
Misc Feature(21)2192..2206(+)
Misc Feature(22)2201..2203(+)
Misc Feature(23)2501..2512(+)
Misc Feature(24)2501..2512(+)
Misc Feature(25)2699..3094(+)
Misc Feature(26)2705..2707(+)
Misc Feature(27)2804..2962(+)
Misc Feature(28)2978..3064(+)
Misc Feature(29)3344..3661(+)
Misc Feature(30)3755..3994(+)
Misc Feature(31)4001..4003(+)
Misc Feature(32)4004..4006(+)
Misc Feature(33)4004..4006(+)
Misc Feature(34)4046..4048(+)
Misc Feature(35)4118..4165(+)
Exon (1)1..114
Gene:BLM
Gene Synonym:
Exon (2)115..216
Gene:BLM
Gene Synonym:
Exon (3)217..917
Gene:BLM
Gene Synonym:
Exon (4)918..1077
Gene:BLM
Gene Synonym:
Exon (5)1078..1205
Gene:BLM
Gene Synonym:
Exon (6)1206..1338
Gene:BLM
Gene Synonym:
Exon (7)1339..2000
Gene:BLM
Gene Synonym:
Exon (8)2001..2192
Gene:BLM
Gene Synonym:
Exon (9)2193..2311
Gene:BLM
Gene Synonym:
Exon (10)2312..2425
Gene:BLM
Gene Synonym:
Exon (11)2426..2524
Gene:BLM
Gene Synonym:
Exon (12)2525..2673
Gene:BLM
Gene Synonym:
Exon (13)2674..2780
Gene:BLM
Gene Synonym:
Exon (14)2781..2941
Gene:BLM
Gene Synonym:
Exon (15)2942..3137
Gene:BLM
Gene Synonym:
Exon (16)3138..3328
Gene:BLM
Gene Synonym:
Exon (17)3329..3476
Gene:BLM
Gene Synonym:
Exon (18)3477..3676
Gene:BLM
Gene Synonym:
Exon (19)3677..3869
Gene:BLM
Gene Synonym:
Exon (20)3870..3992
Gene:BLM
Gene Synonym:
Exon (21)3993..4194
Gene:BLM
Gene Synonym:
Exon (22)4195..4555
Gene:BLM
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000057
Organism Homo sapiens (human)
Definition Homo sapiens Bloom syndrome, RecQ helicase-like (BLM), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000057

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT
AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA
TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA
TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT
GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT
CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT
GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT
GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA
CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC
AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG
CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT
GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT
GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA
AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGGG
AGTAAGAGTG CAAAAATCCA GTCAGGTATA TTTGGAAAAG GATCTGCTTA TTCACGACAC
AATGCCGAAA GACTTTTTAA AAAGCTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT
ATCAATGCCA ATGACCAGGC GATCGCTTAT GTGATGCTCG GAAATAAAGC CCAAACTGTA
CTAAATGGCA ATTTAAAGGT AGACTTTATG GAAACAGAAA ATTCCAGCAG TGTGAAAAAA
CAAAAAGCGT TAGTAGCAAA AGTGTCTCAG AGGGAAGAGA TGGTTAAAAA ATGTCTTGGA
GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GTGTCCATTA CTTCAATATT
TTTAATACCG TCACTCTCAA GAAGCTTGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG
CTTCAAATTG ATGGTGTTAC TGAAGACAAA CTGGAAAAAT ATGGTGCGGA AGTGATTTCA
GTATTACAGA AATACTCTGA ATGGACATCG CCAGCTGAAG ACAGTTCCCC AGGGATAAGC
CTGTCCAGCA GCAGAGGCCC CGGAAGAAGT GCCGCTGAGG AGCTCGACGA GGAAATACCC
GTATCTTCCC ACTACTTTGC AAGTAAAACC AGAAATGAAA GGAAGAGGAA AAAGATGCCA
GCCTCCCAAA GGTCTAAGAG GAGAAAAACT GCTTCCAGTG GTTCCAAGGC AAAGGGGGGG
TCTGCCACAT GTAGAAAGAT ATCTTCCAAA ACGAAATCCT CCAGCATCAT TGGATCCAGT
TCAGCCTCAC ATACTTCTCA AGCGACATCA GGAGCCAATA GCAAATTGGG GATTATGGCT
CCACCGAAGC CTATAAATAG ACCGTTTCTT AAGCCTTCAT ATGCATTCTC ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20445
Clone ID Related Accession (Same CDS sequence) NM_000057 , NM_001287246
Accession Version NM_001287246.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4254bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product Bloom syndrome protein isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD707743.1, BP363946.1, BC143288.1, BC115032.1, DB520927.1 and AC124248.6. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143288.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT
AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA
TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA
TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT
GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT
CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT
GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT
GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA
CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC
AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG
CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT
GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT
GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA
AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGGG
AGTAAGAGTG CAAAAATCCA GTCAGGTATA TTTGGAAAAG GATCTGCTTA TTCACGACAC
AATGCCGAAA GACTTTTTAA AAAGCTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT
ATCAATGCCA ATGACCAGGC GATCGCTTAT GTGATGCTCG GAAATAAAGC CCAAACTGTA
CTAAATGGCA ATTTAAAGGT AGACTTTATG GAAACAGAAA ATTCCAGCAG TGTGAAAAAA
CAAAAAGCGT TAGTAGCAAA AGTGTCTCAG AGGGAAGAGA TGGTTAAAAA ATGTCTTGGA
GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GTGTCCATTA CTTCAATATT
TTTAATACCG TCACTCTCAA GAAGCTTGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG
CTTCAAATTG ATGGTGTTAC TGAAGACAAA CTGGAAAAAT ATGGTGCGGA AGTGATTTCA
GTATTACAGA AATACTCTGA ATGGACATCG CCAGCTGAAG ACAGTTCCCC AGGGATAAGC
CTGTCCAGCA GCAGAGGCCC CGGAAGAAGT GCCGCTGAGG AGCTCGACGA GGAAATACCC
GTATCTTCCC ACTACTTTGC AAGTAAAACC AGAAATGAAA GGAAGAGGAA AAAGATGCCA
GCCTCCCAAA GGTCTAAGAG GAGAAAAACT GCTTCCAGTG GTTCCAAGGC AAAGGGGGGG
TCTGCCACAT GTAGAAAGAT ATCTTCCAAA ACGAAATCCT CCAGCATCAT TGGATCCAGT
TCAGCCTCAC ATACTTCTCA AGCGACATCA GGAGCCAATA GCAAATTGGG GATTATGGCT
CCACCGAAGC CTATAAATAG ACCGTTTCTT AAGCCTTCAT ATGCATTCTC ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001274175.1
CDS229..4482
Misc Feature(1)196..198(+)
Misc Feature(2)310..312(+)
Misc Feature(3)370..372(+)
Misc Feature(4)397..399(+)
Misc Feature(5)568..570(+)
Misc Feature(6)730..732(+)
Misc Feature(7)739..741(+)
Misc Feature(8)796..1050(+)
Misc Feature(9)1129..2028(+)
Misc Feature(10)1300..1302(+)
Misc Feature(11)1339..1461(+)
Misc Feature(12)1483..1485(+)
Misc Feature(13)1492..1494(+)
Misc Feature(14)1504..1506(+)
Misc Feature(15)1723..1725(+)
Misc Feature(16)1750..1752(+)
Misc Feature(17)2197..3657(+)
Misc Feature(18)2275..2724(+)
Misc Feature(19)2302..2316(+)
Misc Feature(20)2611..2622(+)
Misc Feature(21)2611..2622(+)
Misc Feature(22)2809..3204(+)
Misc Feature(23)2815..2817(+)
Misc Feature(24)2914..3072(+)
Misc Feature(25)3088..3174(+)
Misc Feature(26)3454..3771(+)
Misc Feature(27)3865..4104(+)
Misc Feature(28)4111..4113(+)
Misc Feature(29)4114..4116(+)
Misc Feature(30)4156..4158(+)
Misc Feature(31)4228..4275(+)
Exon (1)1..114
Gene:BLM
Gene Synonym:
Exon (2)115..224
Gene:BLM
Gene Synonym:
Exon (3)225..326
Gene:BLM
Gene Synonym:
Exon (4)327..1027
Gene:BLM
Gene Synonym:
Exon (5)1028..1187
Gene:BLM
Gene Synonym:
Exon (6)1188..1315
Gene:BLM
Gene Synonym:
Exon (7)1316..1448
Gene:BLM
Gene Synonym:
Exon (8)1449..2110
Gene:BLM
Gene Synonym:
Exon (9)2111..2302
Gene:BLM
Gene Synonym:
Exon (10)2303..2421
Gene:BLM
Gene Synonym:
Exon (11)2422..2535
Gene:BLM
Gene Synonym:
Exon (12)2536..2634
Gene:BLM
Gene Synonym:
Exon (13)2635..2783
Gene:BLM
Gene Synonym:
Exon (14)2784..2890
Gene:BLM
Gene Synonym:
Exon (15)2891..3051
Gene:BLM
Gene Synonym:
Exon (16)3052..3247
Gene:BLM
Gene Synonym:
Exon (17)3248..3438
Gene:BLM
Gene Synonym:
Exon (18)3439..3586
Gene:BLM
Gene Synonym:
Exon (19)3587..3786
Gene:BLM
Gene Synonym:
Exon (20)3787..3979
Gene:BLM
Gene Synonym:
Exon (21)3980..4102
Gene:BLM
Gene Synonym:
Exon (22)4103..4304
Gene:BLM
Gene Synonym:
Exon (23)4305..4665
Gene:BLM
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001287246
Organism Homo sapiens (human)
Definition Homo sapiens Bloom syndrome, RecQ helicase-like (BLM), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001287246

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT
AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA
TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA
TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT
GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT
CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT
GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT
GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA
CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC
AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG
CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT
GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT
GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA
AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGGG
AGTAAGAGTG CAAAAATCCA GTCAGGTATA TTTGGAAAAG GATCTGCTTA TTCACGACAC
AATGCCGAAA GACTTTTTAA AAAGCTGATA CTTGACAAGA TTTTGGATGA AGACTTATAT
ATCAATGCCA ATGACCAGGC GATCGCTTAT GTGATGCTCG GAAATAAAGC CCAAACTGTA
CTAAATGGCA ATTTAAAGGT AGACTTTATG GAAACAGAAA ATTCCAGCAG TGTGAAAAAA
CAAAAAGCGT TAGTAGCAAA AGTGTCTCAG AGGGAAGAGA TGGTTAAAAA ATGTCTTGGA
GAACTTACAG AAGTCTGCAA ATCTCTGGGG AAAGTTTTTG GTGTCCATTA CTTCAATATT
TTTAATACCG TCACTCTCAA GAAGCTTGCA GAATCTTTAT CTTCTGATCC TGAGGTTTTG
CTTCAAATTG ATGGTGTTAC TGAAGACAAA CTGGAAAAAT ATGGTGCGGA AGTGATTTCA
GTATTACAGA AATACTCTGA ATGGACATCG CCAGCTGAAG ACAGTTCCCC AGGGATAAGC
CTGTCCAGCA GCAGAGGCCC CGGAAGAAGT GCCGCTGAGG AGCTCGACGA GGAAATACCC
GTATCTTCCC ACTACTTTGC AAGTAAAACC AGAAATGAAA GGAAGAGGAA AAAGATGCCA
GCCTCCCAAA GGTCTAAGAG GAGAAAAACT GCTTCCAGTG GTTCCAAGGC AAAGGGGGGG
TCTGCCACAT GTAGAAAGAT ATCTTCCAAA ACGAAATCCT CCAGCATCAT TGGATCCAGT
TCAGCCTCAC ATACTTCTCA AGCGACATCA GGAGCCAATA GCAAATTGGG GATTATGGCT
CCACCGAAGC CTATAAATAG ACCGTTTCTT AAGCCTTCAT ATGCATTCTC ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20391
Clone ID Related Accession (Same CDS sequence) NM_001287247
Accession Version NM_001287247.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product Bloom syndrome protein isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD707743.1, BC062697.1, BC115028.1, BC101567.1, DB520927.1 and AC124248.6. Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 3' coding region compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC115028.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2150385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT
AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA
TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA
TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT
GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT
CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT
GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT
GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA
CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC
AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG
CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT
GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT
GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA
AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGAA
TCTTTATCTT CTGATCCTGA GGTTTTGCTT CAAATTGATG GTGTTACTGA AGACAAACTG
GAAAAATATG GTGCGGAAGT GATTTCAGTA TTACAGAAAT ACTCTGAATG GACATCGCCA
GCTGAAGACA GTTCCCCAGG GATAAGCCTG TCCAGCAGCA GAGGCCCCGG AAGAAGTGCC
GCTGAGGAGC TCGACGAGGA AATACCCGTA TCTTCCCACT ACTTTGCAAG TAAAACCAGA
AATGAAAGGA AGAGGAAAAA GATGCCAGCC TCCCAAAGGT CTAAGAGGAG AAAAACTGCT
TCCAGTGGTT CCAAGGCAAA GGGGGGGTCT GCCACATGTA GAAAGATATC TTCCAAAACG
AAATCCTCCA GCATCATTGG ATCCAGTTCA GCCTCACATA CTTCTCAAGC GACATCAGGA
GCCAATAGCA AATTGGGGAT TATGGCTCCA CCGAAGCCTA TAAATAGACC GTTTCTTAAG
CCTTCATATG CATTCTCATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001274176.1
CDS119..3979
Misc Feature(1)200..202(+)
Misc Feature(2)260..262(+)
Misc Feature(3)287..289(+)
Misc Feature(4)458..460(+)
Misc Feature(5)620..622(+)
Misc Feature(6)629..631(+)
Misc Feature(7)686..940(+)
Misc Feature(8)1019..1918(+)
Misc Feature(9)1190..1192(+)
Misc Feature(10)1229..1351(+)
Misc Feature(11)1373..1375(+)
Misc Feature(12)1382..1384(+)
Misc Feature(13)1394..1396(+)
Misc Feature(14)1613..1615(+)
Misc Feature(15)1640..1642(+)
Misc Feature(16)2087..3487(+)
Misc Feature(17)2165..2614(+)
Misc Feature(18)2192..2206(+)
Misc Feature(19)2501..2512(+)
Misc Feature(20)2501..2512(+)
Misc Feature(21)2699..3094(+)
Misc Feature(22)2705..2707(+)
Misc Feature(23)2804..2962(+)
Misc Feature(24)2978..3064(+)
Misc Feature(25)3443..3601(+)
Exon (1)1..114
Gene:BLM
Gene Synonym:
Exon (2)115..216
Gene:BLM
Gene Synonym:
Exon (3)217..917
Gene:BLM
Gene Synonym:
Exon (4)918..1077
Gene:BLM
Gene Synonym:
Exon (5)1078..1205
Gene:BLM
Gene Synonym:
Exon (6)1206..1338
Gene:BLM
Gene Synonym:
Exon (7)1339..2000
Gene:BLM
Gene Synonym:
Exon (8)2001..2192
Gene:BLM
Gene Synonym:
Exon (9)2193..2311
Gene:BLM
Gene Synonym:
Exon (10)2312..2425
Gene:BLM
Gene Synonym:
Exon (11)2426..2524
Gene:BLM
Gene Synonym:
Exon (12)2525..2673
Gene:BLM
Gene Synonym:
Exon (13)2674..2780
Gene:BLM
Gene Synonym:
Exon (14)2781..2941
Gene:BLM
Gene Synonym:
Exon (15)2942..3137
Gene:BLM
Gene Synonym:
Exon (16)3138..3328
Gene:BLM
Gene Synonym:
Exon (17)3329..3476
Gene:BLM
Gene Synonym:
Exon (18)3477..3599
Gene:BLM
Gene Synonym:
Exon (19)3600..3801
Gene:BLM
Gene Synonym:
Exon (20)3802..4162
Gene:BLM
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001287247
Organism Homo sapiens (human)
Definition Homo sapiens Bloom syndrome, RecQ helicase-like (BLM), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001287247

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGGCTGCTG TTCCTCAAAA TAATCTACAG GAGCAACTAG AACGTCACTC AGCCAGAACA 
CTTAATAATA AATTAAGTCT TTCAAAACCA AAATTTTCAG GTTTCACTTT TAAAAAGAAA
ACATCTTCAG ATAACAATGT ATCTGTAACT AATGTGTCAG TAGCAAAAAC ACCTGTATTA
AGAAATAAAG ATGTTAATGT TACCGAAGAC TTTTCCTTCA GTGAACCTCT ACCCAACACC
ACAAATCAGC AAAGGGTCAA GGACTTCTTT AAAAATGCTC CAGCAGGACA GGAAACACAG
AGAGGTGGAT CAAAATCATT ATTGCCAGAT TTCTTGCAGA CTCCGAAGGA AGTTGTATGC
ACTACCCAAA ACACACCAAC TGTAAAGAAA TCCCGGGATA CTGCTCTCAA GAAATTAGAA
TTTAGTTCTT CACCAGATTC TTTAAGTACC ATCAATGATT GGGATGATAT GGATGACTTT
GATACTTCTG AGACTTCAAA ATCATTTGTT ACACCACCCC AAAGTCACTT TGTAAGAGTA
AGCACTGCTC AGAAATCAAA AAAGGGTAAG AGAAACTTTT TTAAAGCACA GCTTTATACA
ACAAACACAG TAAAGACTGA TTTGCCTCCA CCCTCCTCTG AAAGCGAGCA AATAGATTTG
ACTGAGGAAC AGAAGGATGA CTCAGAATGG TTAAGCAGCG ATGTGATTTG CATCGATGAT
GGCCCCATTG CTGAAGTGCA TATAAATGAA GATGCTCAGG AAAGTGACTC TCTGAAAACT
CATTTGGAAG ATGAAAGAGA TAATAGCGAA AAGAAGAAGA ATTTGGAAGA AGCTGAATTA
CATTCAACTG AGAAAGTTCC ATGTATTGAA TTTGATGATG ATGATTATGA TACGGATTTT
GTTCCACCTT CTCCAGAAGA AATTATTTCT GCTTCTTCTT CCTCTTCAAA ATGCCTTAGT
ACGTTAAAGG ACCTTGACAC CTCTGACAGA AAAGAGGATG TTCTTAGCAC ATCAAAAGAT
CTTTTGTCAA AACCTGAGAA AATGAGTATG CAGGAGCTGA ATCCAGAAAC CAGCACAGAC
TGTGACGCTA GACAGATAAG TTTACAGCAG CAGCTTATTC ATGTGATGGA GCACATCTGT
AAATTAATTG ATACTATTCC TGATGATAAA CTGAAACTTT TGGATTGTGG GAACGAACTG
CTTCAGCAGC GGAACATAAG AAGGAAACTT CTAACGGAAG TAGATTTTAA TAAAAGTGAT
GCCAGTCTTC TTGGCTCATT GTGGAGATAC AGGCCTGATT CACTTGATGG CCCTATGGAG
GGTGATTCCT GCCCTACAGG GAATTCTATG AAGGAGTTAA ATTTTTCACA CCTTCCCTCA
AATTCTGTTT CTCCTGGGGA CTGTTTACTG ACTACCACCC TAGGAAAGAC AGGATTCTCT
GCCACCAGGA AGAATCTTTT TGAAAGGCCT TTATTCAATA CCCATTTACA GAAGTCCTTT
GTAAGTAGCA ACTGGGCTGA AACACCAAGA CTAGGAAAAA AAAATGAAAG CTCTTATTTC
CCAGGAAATG TTCTCACAAG CACTGCTGTG AAAGATCAGA ATAAACATAC TGCTTCAATA
AATGACTTAG AAAGAGAAAC CCAACCTTCC TATGATATTG ATAATTTTGA CATAGATGAC
TTTGATGATG ATGATGACTG GGAAGACATA ATGCATAATT TAGCAGCCAG CAAATCTTCC
ACAGCTGCCT ATCAACCCAT CAAGGAAGGT CGGCCAATTA AATCAGTATC AGAAAGACTT
TCCTCAGCCA AGACAGACTG TCTTCCAGTG TCATCTACTG CTCAAAATAT AAACTTCTCA
GAGTCAATTC AGAATTATAC TGACAAGTCA GCACAAAATT TAGCATCCAG AAATCTGAAA
CATGAGCGTT TCCAAAGTCT TAGTTTTCCT CATACAAAGG AAATGATGAA GATTTTTCAT
AAAAAATTTG GCCTGCATAA TTTTAGAACT AATCAGCTAG AGGCGATCAA TGCTGCACTG
CTTGGTGAAG ACTGTTTTAT CCTGATGCCG ACTGGAGGTG GTAAGAGTTT GTGTTACCAG
CTCCCTGCCT GTGTTTCTCC TGGGGTCACT GTTGTCATTT CTCCCTTGAG ATCACTTATC
GTAGATCAAG TCCAAAAGCT GACTTCCTTG GATATTCCAG CTACATATCT GACAGGTGAT
AAGACTGACT CAGAAGCTAC AAATATTTAC CTCCAGTTAT CAAAAAAAGA CCCAATCATA
AAACTTCTAT ATGTCACTCC AGAAAAGATC TGTGCAAGTA ACAGACTCAT TTCTACTCTG
GAGAATCTCT ATGAGAGGAA GCTCTTGGCA CGTTTTGTTA TTGATGAAGC ACATTGTGTC
AGTCAGTGGG GACATGATTT TCGTCAAGAT TACAAAAGAA TGAATATGCT TCGCCAGAAG
TTTCCTTCTG TTCCGGTGAT GGCTCTTACG GCCACAGCTA ATCCCAGGGT ACAGAAGGAC
ATCCTGACTC AGCTGAAGAT TCTCAGACCT CAGGTGTTTA GCATGAGCTT TAACAGACAT
AATCTGAAAT ACTATGTATT ACCGAAAAAG CCTAAAAAGG TGGCATTTGA TTGCCTAGAA
TGGATCAGAA AGCACCACCC ATATGATTCA GGGATAATTT ACTGCCTCTC CAGGCGAGAA
TGTGACACCA TGGCTGACAC GTTACAGAGA GATGGGCTCG CTGCTCTTGC TTACCATGCT
GGCCTCAGTG ATTCTGCCAG AGATGAAGTG CAGCAGAAGT GGATTAATCA GGATGGCTGT
CAGGTTATCT GTGCTACAAT TGCATTTGGA ATGGGGATTG ACAAACCGGA CGTGCGATTT
GTGATTCATG CATCTCTCCC TAAATCTGTG GAGGGTTACT ACCAAGAATC TGGCAGAGCT
GGAAGAGATG GGGAAATATC TCACTGCCTG CTTTTCTATA CCTATCATGA TGTGACCAGA
CTGAAAAGAC TTATAATGAT GGAAAAAGAT GGAAACCATC ATACAAGAGA AACTCACTTC
AATAATTTGT ATAGCATGGT ACATTACTGT GAAAATATAA CGGAATGCAG GAGAATACAG
CTTTTGGCCT ACTTTGGTGA AAATGGATTT AATCCTGATT TTTGTAAGAA ACACCCAGAT
GTTTCTTGTG ATAATTGCTG TAAAACAAAG GATTATAAAA CAAGAGATGT GACTGACGAT
GTGAAAAGTA TTGTAAGATT TGTTCAAGAA CATAGTTCAT CACAAGGAAT GAGAAATATA
AAACATGTAG GTCCTTCTGG AAGATTTACT ATGAATATGC TGGTCGACAT TTTCTTGGAA
TCTTTATCTT CTGATCCTGA GGTTTTGCTT CAAATTGATG GTGTTACTGA AGACAAACTG
GAAAAATATG GTGCGGAAGT GATTTCAGTA TTACAGAAAT ACTCTGAATG GACATCGCCA
GCTGAAGACA GTTCCCCAGG GATAAGCCTG TCCAGCAGCA GAGGCCCCGG AAGAAGTGCC
GCTGAGGAGC TCGACGAGGA AATACCCGTA TCTTCCCACT ACTTTGCAAG TAAAACCAGA
AATGAAAGGA AGAGGAAAAA GATGCCAGCC TCCCAAAGGT CTAAGAGGAG AAAAACTGCT
TCCAGTGGTT CCAAGGCAAA GGGGGGGTCT GCCACATGTA GAAAGATATC TTCCAAAACG
AAATCCTCCA GCATCATTGG ATCCAGTTCA GCCTCACATA CTTCTCAAGC GACATCAGGA
GCCAATAGCA AATTGGGGAT TATGGCTCCA CCGAAGCCTA TAAATAGACC GTTTCTTAAG
CCTTCATATG CATTCTCATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20947
Clone ID Related Accession (Same CDS sequence) NM_001287248
Accession Version NM_001287248.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3129bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product Bloom syndrome protein isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD707743.1, BC062697.1, BC143280.1, AC021422.25, DA122319.1, BC115032.1, DB520927.1 and AC124248.6. Transcript Variant: This variant (4) uses an alternate splice site in a 5' exon and uses a downstream translation start compared to variant 1. The resulting protein (isoform 3) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143280.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGAGCACA TCTGTAAATT AATTGATACT ATTCCTGATG ATAAACTGAA ACTTTTGGAT 
TGTGGGAACG AACTGCTTCA GCAGCGGAAC ATAAGAAGGA AACTTCTAAC GGAAGTAGAT
TTTAATAAAA GTGATGCCAG TCTTCTTGGC TCATTGTGGA GATACAGGCC TGATTCACTT
GATGGCCCTA TGGAGGGTGA TTCCTGCCCT ACAGGGAATT CTATGAAGGA GTTAAATTTT
TCACACCTTC CCTCAAATTC TGTTTCTCCT GGGGACTGTT TACTGACTAC CACCCTAGGA
AAGACAGGAT TCTCTGCCAC CAGGAAGAAT CTTTTTGAAA GGCCTTTATT CAATACCCAT
TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CAAGACTAGG AAAAAAAAAT
GAAAGCTCTT ATTTCCCAGG AAATGTTCTC ACAAGCACTG CTGTGAAAGA TCAGAATAAA
CATACTGCTT CAATAAATGA CTTAGAAAGA GAAACCCAAC CTTCCTATGA TATTGATAAT
TTTGACATAG ATGACTTTGA TGATGATGAT GACTGGGAAG ACATAATGCA TAATTTAGCA
GCCAGCAAAT CTTCCACAGC TGCCTATCAA CCCATCAAGG AAGGTCGGCC AATTAAATCA
GTATCAGAAA GACTTTCCTC AGCCAAGACA GACTGTCTTC CAGTGTCATC TACTGCTCAA
AATATAAACT TCTCAGAGTC AATTCAGAAT TATACTGACA AGTCAGCACA AAATTTAGCA
TCCAGAAATC TGAAACATGA GCGTTTCCAA AGTCTTAGTT TTCCTCATAC AAAGGAAATG
ATGAAGATTT TTCATAAAAA ATTTGGCCTG CATAATTTTA GAACTAATCA GCTAGAGGCG
ATCAATGCTG CACTGCTTGG TGAAGACTGT TTTATCCTGA TGCCGACTGG AGGTGGTAAG
AGTTTGTGTT ACCAGCTCCC TGCCTGTGTT TCTCCTGGGG TCACTGTTGT CATTTCTCCC
TTGAGATCAC TTATCGTAGA TCAAGTCCAA AAGCTGACTT CCTTGGATAT TCCAGCTACA
TATCTGACAG GTGATAAGAC TGACTCAGAA GCTACAAATA TTTACCTCCA GTTATCAAAA
AAAGACCCAA TCATAAAACT TCTATATGTC ACTCCAGAAA AGATCTGTGC AAGTAACAGA
CTCATTTCTA CTCTGGAGAA TCTCTATGAG AGGAAGCTCT TGGCACGTTT TGTTATTGAT
GAAGCACATT GTGTCAGTCA GTGGGGACAT GATTTTCGTC AAGATTACAA AAGAATGAAT
ATGCTTCGCC AGAAGTTTCC TTCTGTTCCG GTGATGGCTC TTACGGCCAC AGCTAATCCC
AGGGTACAGA AGGACATCCT GACTCAGCTG AAGATTCTCA GACCTCAGGT GTTTAGCATG
AGCTTTAACA GACATAATCT GAAATACTAT GTATTACCGA AAAAGCCTAA AAAGGTGGCA
TTTGATTGCC TAGAATGGAT CAGAAAGCAC CACCCATATG ATTCAGGGAT AATTTACTGC
CTCTCCAGGC GAGAATGTGA CACCATGGCT GACACGTTAC AGAGAGATGG GCTCGCTGCT
CTTGCTTACC ATGCTGGCCT CAGTGATTCT GCCAGAGATG AAGTGCAGCA GAAGTGGATT
AATCAGGATG GCTGTCAGGT TATCTGTGCT ACAATTGCAT TTGGAATGGG GATTGACAAA
CCGGACGTGC GATTTGTGAT TCATGCATCT CTCCCTAAAT CTGTGGAGGG TTACTACCAA
GAATCTGGCA GAGCTGGAAG AGATGGGGAA ATATCTCACT GCCTGCTTTT CTATACCTAT
CATGATGTGA CCAGACTGAA AAGACTTATA ATGATGGAAA AAGATGGAAA CCATCATACA
AGAGAAACTC ACTTCAATAA TTTGTATAGC ATGGTACATT ACTGTGAAAA TATAACGGAA
TGCAGGAGAA TACAGCTTTT GGCCTACTTT GGTGAAAATG GATTTAATCC TGATTTTTGT
AAGAAACACC CAGATGTTTC TTGTGATAAT TGCTGTAAAA CAAAGGATTA TAAAACAAGA
GATGTGACTG ACGATGTGAA AAGTATTGTA AGATTTGTTC AAGAACATAG TTCATCACAA
GGAATGAGAA ATATAAAACA TGTAGGTCCT TCTGGAAGAT TTACTATGAA TATGCTGGTC
GACATTTTCT TGGGGAGTAA GAGTGCAAAA ATCCAGTCAG GTATATTTGG AAAAGGATCT
GCTTATTCAC GACACAATGC CGAAAGACTT TTTAAAAAGC TGATACTTGA CAAGATTTTG
GATGAAGACT TATATATCAA TGCCAATGAC CAGGCGATCG CTTATGTGAT GCTCGGAAAT
AAAGCCCAAA CTGTACTAAA TGGCAATTTA AAGGTAGACT TTATGGAAAC AGAAAATTCC
AGCAGTGTGA AAAAACAAAA AGCGTTAGTA GCAAAAGTGT CTCAGAGGGA AGAGATGGTT
AAAAAATGTC TTGGAGAACT TACAGAAGTC TGCAAATCTC TGGGGAAAGT TTTTGGTGTC
CATTACTTCA ATATTTTTAA TACCGTCACT CTCAAGAAGC TTGCAGAATC TTTATCTTCT
GATCCTGAGG TTTTGCTTCA AATTGATGGT GTTACTGAAG ACAAACTGGA AAAATATGGT
GCGGAAGTGA TTTCAGTATT ACAGAAATAC TCTGAATGGA CATCGCCAGC TGAAGACAGT
TCCCCAGGGA TAAGCCTGTC CAGCAGCAGA GGCCCCGGAA GAAGTGCCGC TGAGGAGCTC
GACGAGGAAA TACCCGTATC TTCCCACTAC TTTGCAAGTA AAACCAGAAA TGAAAGGAAG
AGGAAAAAGA TGCCAGCCTC CCAAAGGTCT AAGAGGAGAA AAACTGCTTC CAGTGGTTCC
AAGGCAAAGG GGGGGTCTGC CACATGTAGA AAGATATCTT CCAAAACGAA ATCCTCCAGC
ATCATTGGAT CCAGTTCAGC CTCACATACT TCTCAAGCGA CATCAGGAGC CAATAGCAAA
TTGGGGATTA TGGCTCCACC GAAGCCTATA AATAGACCGT TTCTTAAGCC TTCATATGCA
TTCTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001274177.1
CDS1410..4538
Misc Feature(1)1350..1352(+)
Misc Feature(2)1410..1517(+)
Misc Feature(3)2253..3713(+)
Misc Feature(4)2331..2780(+)
Misc Feature(5)2358..2372(+)
Misc Feature(6)2667..2678(+)
Misc Feature(7)2865..3260(+)
Misc Feature(8)2970..3128(+)
Misc Feature(9)3144..3230(+)
Misc Feature(10)3510..3827(+)
Misc Feature(11)3921..4160(+)
Exon (1)1..114
Gene:BLM
Gene Synonym:
Exon (2)115..216
Gene:BLM
Gene Synonym:
Exon (3)217..917
Gene:BLM
Gene Synonym:
Exon (4)918..1077
Gene:BLM
Gene Synonym:
Exon (5)1078..1205
Gene:BLM
Gene Synonym:
Exon (6)1206..1504
Gene:BLM
Gene Synonym:
Exon (7)1505..2166
Gene:BLM
Gene Synonym:
Exon (8)2167..2358
Gene:BLM
Gene Synonym:
Exon (9)2359..2477
Gene:BLM
Gene Synonym:
Exon (10)2478..2591
Gene:BLM
Gene Synonym:
Exon (11)2592..2690
Gene:BLM
Gene Synonym:
Exon (12)2691..2839
Gene:BLM
Gene Synonym:
Exon (13)2840..2946
Gene:BLM
Gene Synonym:
Exon (14)2947..3107
Gene:BLM
Gene Synonym:
Exon (15)3108..3303
Gene:BLM
Gene Synonym:
Exon (16)3304..3494
Gene:BLM
Gene Synonym:
Exon (17)3495..3642
Gene:BLM
Gene Synonym:
Exon (18)3643..3842
Gene:BLM
Gene Synonym:
Exon (19)3843..4035
Gene:BLM
Gene Synonym:
Exon (20)4036..4158
Gene:BLM
Gene Synonym:
Exon (21)4159..4360
Gene:BLM
Gene Synonym:
Exon (22)4361..4721
Gene:BLM
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001287248
Organism Homo sapiens (human)
Definition Homo sapiens Bloom syndrome, RecQ helicase-like (BLM), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001287248

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGAGCACA TCTGTAAATT AATTGATACT ATTCCTGATG ATAAACTGAA ACTTTTGGAT 
TGTGGGAACG AACTGCTTCA GCAGCGGAAC ATAAGAAGGA AACTTCTAAC GGAAGTAGAT
TTTAATAAAA GTGATGCCAG TCTTCTTGGC TCATTGTGGA GATACAGGCC TGATTCACTT
GATGGCCCTA TGGAGGGTGA TTCCTGCCCT ACAGGGAATT CTATGAAGGA GTTAAATTTT
TCACACCTTC CCTCAAATTC TGTTTCTCCT GGGGACTGTT TACTGACTAC CACCCTAGGA
AAGACAGGAT TCTCTGCCAC CAGGAAGAAT CTTTTTGAAA GGCCTTTATT CAATACCCAT
TTACAGAAGT CCTTTGTAAG TAGCAACTGG GCTGAAACAC CAAGACTAGG AAAAAAAAAT
GAAAGCTCTT ATTTCCCAGG AAATGTTCTC ACAAGCACTG CTGTGAAAGA TCAGAATAAA
CATACTGCTT CAATAAATGA CTTAGAAAGA GAAACCCAAC CTTCCTATGA TATTGATAAT
TTTGACATAG ATGACTTTGA TGATGATGAT GACTGGGAAG ACATAATGCA TAATTTAGCA
GCCAGCAAAT CTTCCACAGC TGCCTATCAA CCCATCAAGG AAGGTCGGCC AATTAAATCA
GTATCAGAAA GACTTTCCTC AGCCAAGACA GACTGTCTTC CAGTGTCATC TACTGCTCAA
AATATAAACT TCTCAGAGTC AATTCAGAAT TATACTGACA AGTCAGCACA AAATTTAGCA
TCCAGAAATC TGAAACATGA GCGTTTCCAA AGTCTTAGTT TTCCTCATAC AAAGGAAATG
ATGAAGATTT TTCATAAAAA ATTTGGCCTG CATAATTTTA GAACTAATCA GCTAGAGGCG
ATCAATGCTG CACTGCTTGG TGAAGACTGT TTTATCCTGA TGCCGACTGG AGGTGGTAAG
AGTTTGTGTT ACCAGCTCCC TGCCTGTGTT TCTCCTGGGG TCACTGTTGT CATTTCTCCC
TTGAGATCAC TTATCGTAGA TCAAGTCCAA AAGCTGACTT CCTTGGATAT TCCAGCTACA
TATCTGACAG GTGATAAGAC TGACTCAGAA GCTACAAATA TTTACCTCCA GTTATCAAAA
AAAGACCCAA TCATAAAACT TCTATATGTC ACTCCAGAAA AGATCTGTGC AAGTAACAGA
CTCATTTCTA CTCTGGAGAA TCTCTATGAG AGGAAGCTCT TGGCACGTTT TGTTATTGAT
GAAGCACATT GTGTCAGTCA GTGGGGACAT GATTTTCGTC AAGATTACAA AAGAATGAAT
ATGCTTCGCC AGAAGTTTCC TTCTGTTCCG GTGATGGCTC TTACGGCCAC AGCTAATCCC
AGGGTACAGA AGGACATCCT GACTCAGCTG AAGATTCTCA GACCTCAGGT GTTTAGCATG
AGCTTTAACA GACATAATCT GAAATACTAT GTATTACCGA AAAAGCCTAA AAAGGTGGCA
TTTGATTGCC TAGAATGGAT CAGAAAGCAC CACCCATATG ATTCAGGGAT AATTTACTGC
CTCTCCAGGC GAGAATGTGA CACCATGGCT GACACGTTAC AGAGAGATGG GCTCGCTGCT
CTTGCTTACC ATGCTGGCCT CAGTGATTCT GCCAGAGATG AAGTGCAGCA GAAGTGGATT
AATCAGGATG GCTGTCAGGT TATCTGTGCT ACAATTGCAT TTGGAATGGG GATTGACAAA
CCGGACGTGC GATTTGTGAT TCATGCATCT CTCCCTAAAT CTGTGGAGGG TTACTACCAA
GAATCTGGCA GAGCTGGAAG AGATGGGGAA ATATCTCACT GCCTGCTTTT CTATACCTAT
CATGATGTGA CCAGACTGAA AAGACTTATA ATGATGGAAA AAGATGGAAA CCATCATACA
AGAGAAACTC ACTTCAATAA TTTGTATAGC ATGGTACATT ACTGTGAAAA TATAACGGAA
TGCAGGAGAA TACAGCTTTT GGCCTACTTT GGTGAAAATG GATTTAATCC TGATTTTTGT
AAGAAACACC CAGATGTTTC TTGTGATAAT TGCTGTAAAA CAAAGGATTA TAAAACAAGA
GATGTGACTG ACGATGTGAA AAGTATTGTA AGATTTGTTC AAGAACATAG TTCATCACAA
GGAATGAGAA ATATAAAACA TGTAGGTCCT TCTGGAAGAT TTACTATGAA TATGCTGGTC
GACATTTTCT TGGGGAGTAA GAGTGCAAAA ATCCAGTCAG GTATATTTGG AAAAGGATCT
GCTTATTCAC GACACAATGC CGAAAGACTT TTTAAAAAGC TGATACTTGA CAAGATTTTG
GATGAAGACT TATATATCAA TGCCAATGAC CAGGCGATCG CTTATGTGAT GCTCGGAAAT
AAAGCCCAAA CTGTACTAAA TGGCAATTTA AAGGTAGACT TTATGGAAAC AGAAAATTCC
AGCAGTGTGA AAAAACAAAA AGCGTTAGTA GCAAAAGTGT CTCAGAGGGA AGAGATGGTT
AAAAAATGTC TTGGAGAACT TACAGAAGTC TGCAAATCTC TGGGGAAAGT TTTTGGTGTC
CATTACTTCA ATATTTTTAA TACCGTCACT CTCAAGAAGC TTGCAGAATC TTTATCTTCT
GATCCTGAGG TTTTGCTTCA AATTGATGGT GTTACTGAAG ACAAACTGGA AAAATATGGT
GCGGAAGTGA TTTCAGTATT ACAGAAATAC TCTGAATGGA CATCGCCAGC TGAAGACAGT
TCCCCAGGGA TAAGCCTGTC CAGCAGCAGA GGCCCCGGAA GAAGTGCCGC TGAGGAGCTC
GACGAGGAAA TACCCGTATC TTCCCACTAC TTTGCAAGTA AAACCAGAAA TGAAAGGAAG
AGGAAAAAGA TGCCAGCCTC CCAAAGGTCT AAGAGGAGAA AAACTGCTTC CAGTGGTTCC
AAGGCAAAGG GGGGGTCTGC CACATGTAGA AAGATATCTT CCAAAACGAA ATCCTCCAGC
ATCATTGGAT CCAGTTCAGC CTCACATACT TCTCAAGCGA CATCAGGAGC CAATAGCAAA
TTGGGGATTA TGGCTCCACC GAAGCCTATA AATAGACCGT TTCTTAAGCC TTCATATGCA
TTCTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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