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SFTPB cDNA ORF clone, Homo sapiens (human)

Gene Symbol SFTPB
Entrez Gene ID 6439
Full Name surfactant protein B
Synonyms PSP-B, SFTB3, SFTP3, SMDP1, SP-B
General protein information
Preferred Names
pulmonary surfactant-associated protein B
Names
pulmonary surfactant-associated protein B
6 kDa protein
18 kDa pulmonary-surfactant protein
pulmonary surfactant-associated proteolipid SPL(Phe)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2p12-p11.2

Summary This gene encodes the pulmonary-associated surfactant protein B (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period. Alternatively spliced transcript variants encoding the same protein have been identified.[provided by RefSeq, Feb 2010]. lac of sum
Disorder MIM:

178640

Disorder Html: Surfactant metabolism dysfunction, pulmonary, 1, 265120 (3)





The following SFTPB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SFTPB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu09313 XM_005264487 PREDICTED: Homo sapiens surfactant protein B (SFTPB), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu60453 XM_005264488 PREDICTED: Homo sapiens surfactant protein B (SFTPB), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu60454 XM_005264490 PREDICTED: Homo sapiens surfactant protein B (SFTPB), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu09313 NM_198843 Homo sapiens surfactant protein B (SFTPB), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu09313 NM_000542 Homo sapiens surfactant protein B (SFTPB), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu09313
Accession Version XM_005264487.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pulmonary surfactant-associated protein B isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022184.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530367949. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACCAAG CAGGGTACCC AGGCTGCAGA GGTGCCATGG CTGAGTCACA CCTGCTGCAG 
TGGCTGCTGC TGCTGCTGCC CACGCTCTGT GGCCCAGGCA CTGCTGCCTG GACCACCTCA
TCCTTGGCCT GTGCCCAGGG CCCTGAGTTC TGGTGCCAAA GCCTGGAGCA AGCATTGCAG
TGCAGAGCCC TAGGGCATTG CCTACAGGAA GTCTGGGGAC ATGTGGGAGC CGATGACCTA
TGCCAAGAGT GTGAGGACAT CGTCCACATC CTTAACAAGA TGGCCAAGGA GGCCATTTTC
CAGGACACGA TGAGGAAGTT CCTGGAGCAG GAGTGCAACG TCCTCCCCTT GAAGCTGCTC
ATGCCCCAGT GCAACCAAGT GCTTGACGAC TACTTCCCCC TGGTCATCGA CTACTTCCAG
AACCAGACTG ACTCAAACGG CATCTGTATG CACCTGGGCC TGTGCAAATC CCGGCAGCCA
GAGCCAGAGC AGGAGCCAGG GATGTCAGAC CCCCTGCCCA AACCTCTGCG GGACCCTCTG
CCAGACCCTC TGCTGGACAA GCTCGTCCTC CCTGTGCTGC CCGGGGCCCT CCAGGCGAGG
CCTGGGCCTC ACACACAGGA TCTCTCCGAG CAGCAATTCC CCATTCCTCT CCCCTATTGC
TGGCTCTGCA GGGCTCTGAT CAAGCGGATC CAAGCCATGA TTCCCAAGGG TGCGCTAGCT
GTGGCAGTGG CCCAGGTGTG CCGCGTGGTA CCTCTGGTGG CGGGCGGCAT CTGCCAGTGC
CTGGCTGAGC GCTACTCCGT CATCCTGCTC GACACGCTGC TGGGCCGCAT GCTGCCCCAG
CTGGTCTGCC GCCTCGTCCT CCGGTGCTCC ATGGATGACA GCGCTGGCCC AAGGTCGCCG
ACAGGAGAAT GGCTGCCGCG AGACTCTGAG TGCCACCTCT GCATGTCCGT GACCACCCAG
GCCGGGAACA GCAGCGAGCA GGCCATACCA CAGGCAATGC TCCAGGCCTG TGTTGGCTCC
TGGCTGGACA GGGAAAAGTG CAAGCAATTT GTGGAGCAGC ACACGCCCCA GCTGCTGACC
CTGGTGCCCA GGGGCTGGGA TGCCCACACC ACCTGCCAGG CCCTCGGGGT GTGTGGGACC
ATGTCCAGCC CTCTCCAGTG TATCCACAGC CCCGACCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_005264544.1
CDS16..1197
Misc Feature(1)133..234(+)
Misc Feature(2)247..363(+)
Misc Feature(3)250..480(+)
Misc Feature(4)379..474(+)
Misc Feature(5)667..882(+)
Misc Feature(6)940..1149(+)
Misc Feature(7)1045..1143(+)
Translation
Position Chain Variation Link
1 1 a, c dbSNP:768729156
10 10 c, t dbSNP:3024792
12 12 a, g dbSNP:763190494
14 14 c, t dbSNP:35531973
20 20 a, c dbSNP:2077079
21 21 c, t dbSNP:35509268
44 44 g, t dbSNP:745949381
51 51 c, t dbSNP:757550990
92 92 -, tgc dbSNP:147057701
99 99 a, g dbSNP:3024793
114 114 c, g dbSNP:369145822
125 125 g, t dbSNP:561295206
126 126 c, g dbSNP:763238120
129 129 c, t dbSNP:775711586
143 143 c, t dbSNP:765446107
156 156 c, t dbSNP:779654891
160 160 a, g dbSNP:776744560
166 166 c, t dbSNP:771970362
183 183 a, g dbSNP:377675937
187 187 g, t dbSNP:774179232
193 193 a, c dbSNP:768442204
200 200 g, t dbSNP:749043722
209 209 c, g dbSNP:543297835
215 215 a, g dbSNP:779741450
217 217 c, t dbSNP:755877314
238 238 a, g, t dbSNP:780804657
240 240 a, g dbSNP:756849429
241 241 a, g dbSNP:148914290
242 242 a, g dbSNP:764512527
246 246 c, t dbSNP:557499560
255 255 a, g dbSNP:770763661
268 268 a, g dbSNP:541110574
276 276 c, t dbSNP:145895555
277 277 a, g dbSNP:753519344
279 279 c, t dbSNP:748555153
286 286 c, t dbSNP:761090575
298 298 g, t dbSNP:573709240
323 323 a, c, t dbSNP:45488101
324 324 a, g dbSNP:45478492
326 326 c, t dbSNP:757279120
333 333 g, t dbSNP:751745613
336 336 c, t dbSNP:776398334
338 338 c, t dbSNP:765241667
341 341 a, g dbSNP:755008142
342 342 a, g dbSNP:34682912
343 343 c, g dbSNP:564056875
345 345 a, g dbSNP:766040982
348 348 c, g dbSNP:189048961
354 354 c, t dbSNP:772677222
355 355 a, g dbSNP:767270671
363 363 c, t dbSNP:761303463
376 376 a, g dbSNP:773902434
386 386 a, g dbSNP:768183539
402 402 c, t dbSNP:147417469
403 403 a, g dbSNP:45557339
408 408 c, t dbSNP:368293273
409 409 c, t dbSNP:138729391
412 412 -, aa dbSNP:779795223
412 412 c, g dbSNP:141905538
412 412 c, gaa dbSNP:35328240
423 423 c, t dbSNP:757546697
424 424 a, g dbSNP:140667432
426 426 -, c dbSNP:766821377
427 427 g, t dbSNP:184305471
438 438 c, g dbSNP:758573590
439 439 c, g dbSNP:753771008
443 443 c, t dbSNP:1130866
447 447 c, t dbSNP:529554098
453 453 c, t dbSNP:374131015
454 454 a, g dbSNP:35373464
463 463 a, g dbSNP:751412409
464 464 c, t dbSNP:777381019
468 468 c, t dbSNP:758084893
471 471 a, g dbSNP:752321943
473 473 a, g dbSNP:549877442
487 487 c, t dbSNP:531280852
488 488 a, c, g dbSNP:367831550
510 510 a, g, t dbSNP:773765909
523 523 c, g dbSNP:778541429
525 525 a, c dbSNP:762058470
526 526 c, g, t dbSNP:768712184
530 530 c, t dbSNP:375203471
536 536 a, c dbSNP:781094026
539 539 a, c dbSNP:770903531
544 544 c, t dbSNP:370715446
545 545 a, g dbSNP:370750455
561 561 a, c dbSNP:149904344
567 567 a, c, g dbSNP:377624872
570 570 a, g dbSNP:778434631
571 571 c, g dbSNP:548370069
576 576 c, g dbSNP:754507327
577 577 c, t dbSNP:3024801
579 579 c, t dbSNP:147209702
580 580 a, c, g dbSNP:34550459
582 582 c, t dbSNP:751001412
594 594 a, g dbSNP:767932734
595 595 c, t dbSNP:138274558
597 597 c, t dbSNP:45505791
598 598 a, g dbSNP:184494733
599 599 a, g dbSNP:35524245
601 601 a, c, g dbSNP:778119902
601 601 -, g dbSNP:749997825
611 611 c, t dbSNP:756571077
612 612 a, g dbSNP:543913426
615 615 a, g dbSNP:773147825
620 620 a, g dbSNP:771834627
621 621 c, g dbSNP:141123597
623 623 c, t dbSNP:377545629
627 627 c, t dbSNP:45601634
631 631 c, g dbSNP:748780640
634 634 a, g dbSNP:267599489
642 642 c, t dbSNP:45530632
643 643 a, g dbSNP:747957177
645 645 a, g dbSNP:774056762
648 648 a, g dbSNP:768532112
654 654 a, c dbSNP:190610109
658 658 a, g dbSNP:149956217
662 662 c, g, t dbSNP:150799880
663 663 c, t dbSNP:745426045
666 666 c, t dbSNP:781706067
671 671 a, g dbSNP:757742686
684 684 c, t dbSNP:35049407
700 700 a, c, t dbSNP:758775152
701 701 a, g dbSNP:762917358
713 713 c, t dbSNP:765510340
727 727 g, t dbSNP:753120082
728 728 c, t dbSNP:369801396
729 729 a, g dbSNP:550631097
733 733 c, g dbSNP:2854728
739 739 g, t dbSNP:754123363
745 745 -, g dbSNP:145611216
746 746 c, t dbSNP:766325255
754 754 c, t dbSNP:760826724
757 757 c, t dbSNP:137853202
759 759 c, t dbSNP:367661082
760 760 a, g dbSNP:762818048
763 763 a, g dbSNP:775462975
776 776 a, c, t dbSNP:759213352
777 777 a, g dbSNP:144359413
788 788 a, g dbSNP:770278561
792 792 a, g dbSNP:727503418
795 795 a, c dbSNP:746597889
798 798 a, g dbSNP:773578596
805 805 c, t dbSNP:772619023
806 806 a, g dbSNP:748482999
807 807 a, c dbSNP:779211401
813 813 c, t dbSNP:755360810
814 814 a, g dbSNP:186576601
816 816 a, c, t dbSNP:756130868
825 825 c, g, t dbSNP:764088882
826 826 c, g dbSNP:758367650
830 830 c, t dbSNP:546778802
831 831 a, g dbSNP:528288865
833 833 g, t dbSNP:759379195
837 837 c, g dbSNP:138686998
841 841 c, t dbSNP:45595337
842 842 a, g, t dbSNP:371267944
849 849 g, t dbSNP:771604538
852 852 c, t dbSNP:565870876
866 866 a, g, t dbSNP:3024809
871 871 a, g dbSNP:768946529
877 877 c, t dbSNP:542291993
878 878 a, g dbSNP:201996765
890 890 a, g dbSNP:756396147
894 894 c, t dbSNP:3024810
896 896 g, t dbSNP:45551431
898 898 a, g dbSNP:36210375
911 911 c, t dbSNP:751911024
912 912 a, g dbSNP:747055391
914 914 c, t dbSNP:35076740
915 915 a, g dbSNP:772068983
920 920 a, c, g dbSNP:45504597
926 926 c, g dbSNP:755937427
933 933 a, g dbSNP:750280427
935 935 a, g dbSNP:780787602
939 939 a, c, t dbSNP:538947359
944 944 a, c dbSNP:763598761
948 948 c, t dbSNP:762371851
951 951 c, g dbSNP:571612847
963 963 c, t dbSNP:753242123
964 964 a, g dbSNP:765720471
969 969 c, t dbSNP:546905132
973 973 c, t dbSNP:777177263
979 979 a, g dbSNP:201986026
990 990 c, t dbSNP:529085827
1006 1006 a, c dbSNP:561113178
1011 1011 a, g dbSNP:772126727
1015 1015 c, t dbSNP:527424726
1023 1023 a, c dbSNP:548414751
1035 1035 c, t dbSNP:775488654
1037 1037 a, g dbSNP:769758832
1053 1053 c, g dbSNP:745832819
1055 1055 a, g dbSNP:767694183
1061 1061 a, t dbSNP:143687195
1079 1079 c, t dbSNP:567506649
1080 1080 a, g dbSNP:45606043
1081 1081 a, c dbSNP:745744765
1082 1082 -, gcc dbSNP:781302871
1092 1092 a, c, g dbSNP:368681670
1097 1097 g, t dbSNP:746836305
1101 1101 a, g dbSNP:777459025
1135 1135 g, t dbSNP:780688071
1140 1140 c, t dbSNP:756623575
1141 1141 a, g dbSNP:750958534
1146 1146 a, g dbSNP:781349851
1148 1148 g, t dbSNP:757642887
1160 1160 c, t dbSNP:374932425
1164 1164 a, c dbSNP:370528656
1166 1166 c, g dbSNP:368016212
1169 1169 -, tc dbSNP:751886630
1173 1173 c, g dbSNP:551956264
1187 1187 c, g dbSNP:144831319
1189 1189 a, g dbSNP:370785493
1200 1200 a, t dbSNP:773204473
1206 1206 c, t dbSNP:771704983
1209 1209 a, c dbSNP:45529633
1212 1212 c, t dbSNP:371255380
1215 1215 a, g dbSNP:774139517
1216 1216 g, t dbSNP:768460155
1286 1286 a, g dbSNP:555613505
1291 1291 c, g dbSNP:369263105
1326 1326 a, c dbSNP:56811788
1335 1335 a, g dbSNP:543912566
1375 1375 a, g dbSNP:576528370
1387 1387 g, t dbSNP:1804246
1397 1397 a, g dbSNP:114691084
1399 1399 c, g dbSNP:9752
1413 1413 a, g dbSNP:566196796
1422 1422 c, t dbSNP:555632775
1476 1476 a, g dbSNP:3024822
1477 1477 c, t dbSNP:568626838
1494 1494 c, t dbSNP:549483539
1497 1497 c, t dbSNP:188417591
1559 1559 c, t dbSNP:570119766
1567 1567 c, t dbSNP:551707788
1592 1592 c, t dbSNP:3024823
1613 1613 a, g dbSNP:560227586
1628 1628 c, t dbSNP:745310719
1629 1629 c, t dbSNP:545321542
1677 1677 c, t dbSNP:749387121
1686 1686 a, t dbSNP:569810989
1719 1719 a, g dbSNP:578093456
1721 1721 a, g dbSNP:182975929
1724 1724 c, t dbSNP:3024824
1732 1732 -, taga dbSNP:369576391
1759 1759 g, t dbSNP:191130863
1773 1773 a, g dbSNP:562675679
1787 1787 a, g dbSNP:185386014
1795 1795 g, t dbSNP:148430793
1796 1796 a, g dbSNP:571402858
1809 1809 c, t dbSNP:756851833
1834 1834 c, t dbSNP:564476672
1835 1835 a, g dbSNP:144475205
1836 1836 a, g dbSNP:572397585
1845 1845 g, t dbSNP:554205820
1853 1853 a, g dbSNP:367597303
1858 1858 a, g dbSNP:779988988
1890 1890 -, atg dbSNP:3024825
1890 1890 cat, g dbSNP:367798135
1890 1890 c, g dbSNP:574947393
1895 1895 -, ta dbSNP:147067432
1901 1901 c, g dbSNP:3024826
1925 1925 a, g dbSNP:758155280
1946 1946 g, t dbSNP:537548634
1959 1959 a, g dbSNP:367966703
1997 1997 c, g dbSNP:3024827
2001 2001 -, g dbSNP:35502847
2005 2005 a, g dbSNP:764992096
2052 2052 a, g dbSNP:11544469
2053 2053 a, g dbSNP:7316

Target ORF information:

RefSeq Version XM_005264487
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens surfactant protein B (SFTPB), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005264487

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACCAAG CAGGGTACCC AGGCTGCAGA GGTGCCATGG CTGAGTCACA CCTGCTGCAG 
TGGCTGCTGC TGCTGCTGCC CACGCTCTGT GGCCCAGGCA CTGCTGCCTG GACCACCTCA
TCCTTGGCCT GTGCCCAGGG CCCTGAGTTC TGGTGCCAAA GCCTGGAGCA AGCATTGCAG
TGCAGAGCCC TAGGGCATTG CCTACAGGAA GTCTGGGGAC ATGTGGGAGC CGATGACCTA
TGCCAAGAGT GTGAGGACAT CGTCCACATC CTTAACAAGA TGGCCAAGGA GGCCATTTTC
CAGGACACGA TGAGGAAGTT CCTGGAGCAG GAGTGCAACG TCCTCCCCTT GAAGCTGCTC
ATGCCCCAGT GCAACCAAGT GCTTGACGAC TACTTCCCCC TGGTCATCGA CTACTTCCAG
AACCAGACTG ACTCAAACGG CATCTGTATG CACCTGGGCC TGTGCAAATC CCGGCAGCCA
GAGCCAGAGC AGGAGCCAGG GATGTCAGAC CCCCTGCCCA AACCTCTGCG GGACCCTCTG
CCAGACCCTC TGCTGGACAA GCTCGTCCTC CCTGTGCTGC CCGGGGCCCT CCAGGCGAGG
CCTGGGCCTC ACACACAGGA TCTCTCCGAG CAGCAATTCC CCATTCCTCT CCCCTATTGC
TGGCTCTGCA GGGCTCTGAT CAAGCGGATC CAAGCCATGA TTCCCAAGGG TGCGCTAGCT
GTGGCAGTGG CCCAGGTGTG CCGCGTGGTA CCTCTGGTGG CGGGCGGCAT CTGCCAGTGC
CTGGCTGAGC GCTACTCCGT CATCCTGCTC GACACGCTGC TGGGCCGCAT GCTGCCCCAG
CTGGTCTGCC GCCTCGTCCT CCGGTGCTCC ATGGATGACA GCGCTGGCCC AAGGTCGCCG
ACAGGAGAAT GGCTGCCGCG AGACTCTGAG TGCCACCTCT GCATGTCCGT GACCACCCAG
GCCGGGAACA GCAGCGAGCA GGCCATACCA CAGGCAATGC TCCAGGCCTG TGTTGGCTCC
TGGCTGGACA GGGAAAAGTG CAAGCAATTT GTGGAGCAGC ACACGCCCCA GCTGCTGACC
CTGGTGCCCA GGGGCTGGGA TGCCCACACC ACCTGCCAGG CCCTCGGGGT GTGTGGGACC
ATGTCCAGCC CTCTCCAGTG TATCCACAGC CCCGACCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60453
Accession Version XM_005264488.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1134bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pulmonary surfactant-associated protein B isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022184.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530367951. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGAGT CACACCTGCT GCAGTGGCTG CTGCTGCTGC TGCCCACGCT CTGTGGCCCA 
GGCACTGCTG CCTGGACCAC CTCATCCTTG GCCTGTGCCC AGGGCCCTGA GTTCTGGTGC
CAAAGCCTGG AGCAAGCATT GCAGTGCAGA GCCCTAGGGC ATTGCCTACA GGAAGTCTGG
GGACATGTGG GAGCCGATGA CCTATGCCAA GAGTGTGAGG ACATCGTCCA CATCCTTAAC
AAGATGGCCA AGGAGGCCAT TTTCCAGGAC ACGATGAGGA AGTTCCTGGA GCAGGAGTGC
AACGTCCTCC CCTTGAAGCT GCTCATGCCC CAGTGCAACC AAGTGCTTGA CGACTACTTC
CCCCTGGTCA TCGACTACTT CCAGAACCAG ACTGACTCAA ACGGCATCTG TATGCACCTG
GGCCTGTGCA AATCCCGGCA GCCAGAGCCA GAGCAGGAGC CAGGGATGTC AGACCCCCTG
CCCAAACCTC TGCGGGACCC TCTGCCAGAC CCTCTGCTGG ACAAGCTCGT CCTCCCTGTG
CTGCCCGGGG CCCTCCAGGC GAGGCCTGGG CCTCACACAC AGGATCTCTC CGAGCAGCAA
TTCCCCATTC CTCTCCCCTA TTGCTGGCTC TGCAGGGCTC TGATCAAGCG GATCCAAGCC
ATGATTCCCA AGGGTGCGCT AGCTGTGGCA GTGGCCCAGG TGTGCCGCGT GGTACCTCTG
GTGGCGGGCG GCATCTGCCA GTGCCTGGCT GAGCGCTACT CCGTCATCCT GCTCGACACG
CTGCTGGGCC GCATGCTGCC CCAGCTGGTC TGCCGCCTCG TCCTCCGGTG CTCCATGGAT
GACAGCGCTG GCCCAAGAGA ATGGCTGCCG CGAGACTCTG AGTGCCACCT CTGCATGTCC
GTGACCACCC AGGCCGGGAA CAGCAGCGAG CAGGCCATAC CACAGGCAAT GCTCCAGGCC
TGTGTTGGCT CCTGGCTGGA CAGGGAAAAG TGCAAGCAAT TTGTGGAGCA GCACACGCCC
CAGCTGCTGA CCCTGGTGCC CAGGGGCTGG GATGCCCACA CCACCTGCCA GGCCCTCGGG
GTGTGTGGGA CCATGTCCAG CCCTCTCCAG TGTATCCACA GCCCCGACCT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_005264545.2
CDS17..1150
Misc Feature(1)98..199(+)
Misc Feature(2)212..328(+)
Misc Feature(3)215..445(+)
Misc Feature(4)344..439(+)
Misc Feature(5)632..847(+)
Misc Feature(6)893..1102(+)
Misc Feature(7)998..1096(+)
Translation
Position Chain Variation Link
9 9 g, t dbSNP:745949381
16 16 c, t dbSNP:757550990
57 57 -, tgc dbSNP:147057701
64 64 a, g dbSNP:3024793
79 79 c, g dbSNP:369145822
90 90 g, t dbSNP:561295206
91 91 c, g dbSNP:763238120
94 94 c, t dbSNP:775711586
108 108 c, t dbSNP:765446107
121 121 c, t dbSNP:779654891
125 125 a, g dbSNP:776744560
131 131 c, t dbSNP:771970362
148 148 a, g dbSNP:377675937
152 152 g, t dbSNP:774179232
158 158 a, c dbSNP:768442204
165 165 g, t dbSNP:749043722
174 174 c, g dbSNP:543297835
180 180 a, g dbSNP:779741450
182 182 c, t dbSNP:755877314
203 203 a, g, t dbSNP:780804657
205 205 a, g dbSNP:756849429
206 206 a, g dbSNP:148914290
207 207 a, g dbSNP:764512527
211 211 c, t dbSNP:557499560
220 220 a, g dbSNP:770763661
233 233 a, g dbSNP:541110574
241 241 c, t dbSNP:145895555
242 242 a, g dbSNP:753519344
244 244 c, t dbSNP:748555153
251 251 c, t dbSNP:761090575
263 263 g, t dbSNP:573709240
288 288 a, c, t dbSNP:45488101
289 289 a, g dbSNP:45478492
291 291 c, t dbSNP:757279120
298 298 g, t dbSNP:751745613
301 301 c, t dbSNP:776398334
303 303 c, t dbSNP:765241667
306 306 a, g dbSNP:755008142
307 307 a, g dbSNP:34682912
308 308 c, g dbSNP:564056875
310 310 a, g dbSNP:766040982
313 313 c, g dbSNP:189048961
319 319 c, t dbSNP:772677222
320 320 a, g dbSNP:767270671
328 328 c, t dbSNP:761303463
341 341 a, g dbSNP:773902434
351 351 a, g dbSNP:768183539
367 367 c, t dbSNP:147417469
368 368 a, g dbSNP:45557339
373 373 c, t dbSNP:368293273
374 374 c, t dbSNP:138729391
377 377 -, aa dbSNP:779795223
377 377 c, g dbSNP:141905538
377 377 c, gaa dbSNP:35328240
388 388 c, t dbSNP:757546697
389 389 a, g dbSNP:140667432
391 391 -, c dbSNP:766821377
392 392 g, t dbSNP:184305471
403 403 c, g dbSNP:758573590
404 404 c, g dbSNP:753771008
408 408 c, t dbSNP:1130866
412 412 c, t dbSNP:529554098
418 418 c, t dbSNP:374131015
419 419 a, g dbSNP:35373464
428 428 a, g dbSNP:751412409
429 429 c, t dbSNP:777381019
433 433 c, t dbSNP:758084893
436 436 a, g dbSNP:752321943
438 438 a, g dbSNP:549877442
452 452 c, t dbSNP:531280852
453 453 a, c, g dbSNP:367831550
475 475 a, g, t dbSNP:773765909
488 488 c, g dbSNP:778541429
490 490 a, c dbSNP:762058470
491 491 c, g, t dbSNP:768712184
495 495 c, t dbSNP:375203471
501 501 a, c dbSNP:781094026
504 504 a, c dbSNP:770903531
509 509 c, t dbSNP:370715446
510 510 a, g dbSNP:370750455
526 526 a, c dbSNP:149904344
532 532 a, c, g dbSNP:377624872
535 535 a, g dbSNP:778434631
536 536 c, g dbSNP:548370069
541 541 c, g dbSNP:754507327
542 542 c, t dbSNP:3024801
544 544 c, t dbSNP:147209702
545 545 a, c, g dbSNP:34550459
547 547 c, t dbSNP:751001412
559 559 a, g dbSNP:767932734
560 560 c, t dbSNP:138274558
562 562 c, t dbSNP:45505791
563 563 a, g dbSNP:184494733
564 564 a, g dbSNP:35524245
566 566 a, c, g dbSNP:778119902
566 566 -, g dbSNP:749997825
576 576 c, t dbSNP:756571077
577 577 a, g dbSNP:543913426
580 580 a, g dbSNP:773147825
585 585 a, g dbSNP:771834627
586 586 c, g dbSNP:141123597
588 588 c, t dbSNP:377545629
592 592 c, t dbSNP:45601634
596 596 c, g dbSNP:748780640
599 599 a, g dbSNP:267599489
607 607 c, t dbSNP:45530632
608 608 a, g dbSNP:747957177
610 610 a, g dbSNP:774056762
613 613 a, g dbSNP:768532112
619 619 a, c dbSNP:190610109
623 623 a, g dbSNP:149956217
627 627 c, g, t dbSNP:150799880
628 628 c, t dbSNP:745426045
631 631 c, t dbSNP:781706067
636 636 a, g dbSNP:757742686
649 649 c, t dbSNP:35049407
665 665 a, c, t dbSNP:758775152
666 666 a, g dbSNP:762917358
678 678 c, t dbSNP:765510340
692 692 g, t dbSNP:753120082
693 693 c, t dbSNP:369801396
694 694 a, g dbSNP:550631097
698 698 c, g dbSNP:2854728
704 704 g, t dbSNP:754123363
710 710 -, g dbSNP:145611216
711 711 c, t dbSNP:766325255
719 719 c, t dbSNP:760826724
722 722 c, t dbSNP:137853202
724 724 c, t dbSNP:367661082
725 725 a, g dbSNP:762818048
728 728 a, g dbSNP:775462975
741 741 a, c, t dbSNP:759213352
742 742 a, g dbSNP:144359413
753 753 a, g dbSNP:770278561
757 757 a, g dbSNP:727503418
760 760 a, c dbSNP:746597889
763 763 a, g dbSNP:773578596
770 770 c, t dbSNP:772619023
771 771 a, g dbSNP:748482999
772 772 a, c dbSNP:779211401
778 778 c, t dbSNP:755360810
779 779 a, g dbSNP:186576601
781 781 a, c, t dbSNP:756130868
790 790 c, g, t dbSNP:764088882
791 791 c, g dbSNP:758367650
795 795 c, t dbSNP:546778802
796 796 a, g dbSNP:528288865
798 798 g, t dbSNP:759379195
802 802 c, g dbSNP:138686998
806 806 c, t dbSNP:45595337
807 807 a, g, t dbSNP:371267944
814 814 g, t dbSNP:771604538
817 817 c, t dbSNP:565870876
831 831 a, g, t dbSNP:3024809
836 836 a, g dbSNP:768946529
842 842 c, t dbSNP:542291993
843 843 a, g dbSNP:201996765
855 855 a, g dbSNP:756396147
859 859 c, t dbSNP:3024810
861 861 g, t dbSNP:45551431
863 863 a, g dbSNP:36210375
873 873 a, c, g dbSNP:45504597
879 879 c, g dbSNP:755937427
886 886 a, g dbSNP:750280427
888 888 a, g dbSNP:780787602
892 892 a, c, t dbSNP:538947359
897 897 a, c dbSNP:763598761
901 901 c, t dbSNP:762371851
904 904 c, g dbSNP:571612847
916 916 c, t dbSNP:753242123
917 917 a, g dbSNP:765720471
922 922 c, t dbSNP:546905132
926 926 c, t dbSNP:777177263
932 932 a, g dbSNP:201986026
943 943 c, t dbSNP:529085827
959 959 a, c dbSNP:561113178
964 964 a, g dbSNP:772126727
968 968 c, t dbSNP:527424726
976 976 a, c dbSNP:548414751
988 988 c, t dbSNP:775488654
990 990 a, g dbSNP:769758832
1006 1006 c, g dbSNP:745832819
1008 1008 a, g dbSNP:767694183
1014 1014 a, t dbSNP:143687195
1032 1032 c, t dbSNP:567506649
1033 1033 a, g dbSNP:45606043
1034 1034 a, c dbSNP:745744765
1035 1035 -, gcc dbSNP:781302871
1045 1045 a, c, g dbSNP:368681670
1050 1050 g, t dbSNP:746836305
1054 1054 a, g dbSNP:777459025
1088 1088 g, t dbSNP:780688071
1093 1093 c, t dbSNP:756623575
1094 1094 a, g dbSNP:750958534
1099 1099 a, g dbSNP:781349851
1101 1101 g, t dbSNP:757642887
1113 1113 c, t dbSNP:374932425
1117 1117 a, c dbSNP:370528656
1119 1119 c, g dbSNP:368016212
1122 1122 -, tc dbSNP:751886630
1126 1126 c, g dbSNP:551956264
1140 1140 c, g dbSNP:144831319
1142 1142 a, g dbSNP:370785493
1153 1153 a, t dbSNP:773204473
1159 1159 c, t dbSNP:771704983
1162 1162 a, c dbSNP:45529633
1165 1165 c, t dbSNP:371255380
1168 1168 a, g dbSNP:774139517
1169 1169 g, t dbSNP:768460155
1195 1195 a, g dbSNP:555613505
1200 1200 c, g dbSNP:369263105
1235 1235 a, c dbSNP:56811788
1244 1244 a, g dbSNP:543912566
1284 1284 a, g dbSNP:576528370
1296 1296 g, t dbSNP:1804246
1306 1306 a, g dbSNP:114691084
1308 1308 c, g dbSNP:9752
1322 1322 a, g dbSNP:566196796
1331 1331 c, t dbSNP:555632775
1385 1385 a, g dbSNP:3024822
1386 1386 c, t dbSNP:568626838
1403 1403 c, t dbSNP:549483539
1406 1406 c, t dbSNP:188417591
1468 1468 c, t dbSNP:570119766
1476 1476 c, t dbSNP:551707788
1501 1501 c, t dbSNP:3024823
1522 1522 a, g dbSNP:560227586
1537 1537 c, t dbSNP:745310719
1538 1538 c, t dbSNP:545321542
1586 1586 c, t dbSNP:749387121
1595 1595 a, t dbSNP:569810989
1628 1628 a, g dbSNP:578093456
1630 1630 a, g dbSNP:182975929
1633 1633 c, t dbSNP:3024824
1641 1641 -, taga dbSNP:369576391
1668 1668 g, t dbSNP:191130863
1682 1682 a, g dbSNP:562675679
1696 1696 a, g dbSNP:185386014
1704 1704 g, t dbSNP:148430793
1705 1705 a, g dbSNP:571402858
1718 1718 c, t dbSNP:756851833
1743 1743 c, t dbSNP:564476672
1744 1744 a, g dbSNP:144475205
1745 1745 a, g dbSNP:572397585
1754 1754 g, t dbSNP:554205820
1762 1762 a, g dbSNP:367597303
1767 1767 a, g dbSNP:779988988
1799 1799 -, atg dbSNP:3024825
1799 1799 cat, g dbSNP:367798135
1799 1799 c, g dbSNP:574947393
1804 1804 -, ta dbSNP:147067432
1810 1810 c, g dbSNP:3024826
1834 1834 a, g dbSNP:758155280
1855 1855 g, t dbSNP:537548634
1868 1868 a, g dbSNP:367966703
1906 1906 c, g dbSNP:3024827
1910 1910 -, g dbSNP:35502847
1914 1914 a, g dbSNP:764992096
1961 1961 a, g dbSNP:11544469
1962 1962 a, g dbSNP:7316

Target ORF information:

RefSeq Version XM_005264488
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens surfactant protein B (SFTPB), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005264488

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGAGT CACACCTGCT GCAGTGGCTG CTGCTGCTGC TGCCCACGCT CTGTGGCCCA 
GGCACTGCTG CCTGGACCAC CTCATCCTTG GCCTGTGCCC AGGGCCCTGA GTTCTGGTGC
CAAAGCCTGG AGCAAGCATT GCAGTGCAGA GCCCTAGGGC ATTGCCTACA GGAAGTCTGG
GGACATGTGG GAGCCGATGA CCTATGCCAA GAGTGTGAGG ACATCGTCCA CATCCTTAAC
AAGATGGCCA AGGAGGCCAT TTTCCAGGAC ACGATGAGGA AGTTCCTGGA GCAGGAGTGC
AACGTCCTCC CCTTGAAGCT GCTCATGCCC CAGTGCAACC AAGTGCTTGA CGACTACTTC
CCCCTGGTCA TCGACTACTT CCAGAACCAG ACTGACTCAA ACGGCATCTG TATGCACCTG
GGCCTGTGCA AATCCCGGCA GCCAGAGCCA GAGCAGGAGC CAGGGATGTC AGACCCCCTG
CCCAAACCTC TGCGGGACCC TCTGCCAGAC CCTCTGCTGG ACAAGCTCGT CCTCCCTGTG
CTGCCCGGGG CCCTCCAGGC GAGGCCTGGG CCTCACACAC AGGATCTCTC CGAGCAGCAA
TTCCCCATTC CTCTCCCCTA TTGCTGGCTC TGCAGGGCTC TGATCAAGCG GATCCAAGCC
ATGATTCCCA AGGGTGCGCT AGCTGTGGCA GTGGCCCAGG TGTGCCGCGT GGTACCTCTG
GTGGCGGGCG GCATCTGCCA GTGCCTGGCT GAGCGCTACT CCGTCATCCT GCTCGACACG
CTGCTGGGCC GCATGCTGCC CCAGCTGGTC TGCCGCCTCG TCCTCCGGTG CTCCATGGAT
GACAGCGCTG GCCCAAGAGA ATGGCTGCCG CGAGACTCTG AGTGCCACCT CTGCATGTCC
GTGACCACCC AGGCCGGGAA CAGCAGCGAG CAGGCCATAC CACAGGCAAT GCTCCAGGCC
TGTGTTGGCT CCTGGCTGGA CAGGGAAAAG TGCAAGCAAT TTGTGGAGCA GCACACGCCC
CAGCTGCTGA CCCTGGTGCC CAGGGGCTGG GATGCCCACA CCACCTGCCA GGCCCTCGGG
GTGTGTGGGA CCATGTCCAG CCCTCTCCAG TGTATCCACA GCCCCGACCT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60454
Accession Version XM_005264490.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pulmonary surfactant-associated protein B isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022184.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578803094. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGAGT CACACCTGCT GCAGTGGCTG CTGCTGCTGC TGCCCACGCT CTGTGGCCCA 
GGCACTGCTG CCTGGACCAC CTCATCCTTG GCCTGTGCCC AGGGCCCTGA GTTCTGGTGC
CAAAGCCTGG AGCAAGCATT GCAGTGCAGA GCCCTAGGGC ATTGCCTACA GGAAGTCTGG
GGACATGTGG GAGCCGATGA CCTATGCCAA GAGTGTGAGG ACATCGTCCA CATCCTTAAC
AAGATGGCCA AGGAGGCCAT TTTCCAGGAC ACGATGAGGA AGTTCCTGGA GCAGGAGTGC
AACGTCCTCC CCTTGAAGCT GCTCATGCCC CAGTGCAACC AAGTGCTTGA CGACTACTTC
CCCCTGGTCA TCGACTACTT CCAGAACCAG ACTGACTCAA ACGGCATCTG TATGCACCTG
GGCCTGTGCA AATCCCGGCA GCCAGAGCCA GAGCAGGAGC CAGGGATGTC AGACCCCCTG
CCCAAACCTC TGCGGGACCC TCTGCCAGAC CCTCTGCTGG ACAAGCTCGT CCTCCCTGTG
CTGCCCGGGG CCCTCCAGGC GAGGCCTGGG CCTCACACAC AGGATCTCTC CGAGCAGCAA
TTCCCCATTC CTCTCCCCTA TTGCTGGCTC TGCAGGGCTC TGATCAAGCG GATCCAAGCC
ATGATTCCCA AGGGTGCGCT AGCTGTGGCA GTGGCCCAGG TGTGCCGCGT GGTACCTCTG
GTGGCGGGCG GCATCTGCCA GTGCCTGGCT GAGCGCTACT CCGTCATCCT GCTCGACACG
CTGCTGGGCC GCATGCTGCC CCAGCTGGTC TGCCGCCTCG TCCTCCGGTG CTCCATGGAT
GACAGCGCTG GCCCAAGGTC GCCGACAGGA GAATGGCTGC CGCGAGACTC TGAGTGCCAC
CTCTGCATGT CCGTGACCAC CCAGGCCGGG AACAGCAGCG AGCAGGCCAT ACCACAGGCA
ATGCTCCAGG CCTGTGTTGG CTCCTGGCTG GACAGGGAAA AGCTGGCTCC AGTTTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_005264547.2
CDS17..1036
Misc Feature(1)98..199(+)
Misc Feature(2)212..328(+)
Misc Feature(3)215..445(+)
Misc Feature(4)344..439(+)
Misc Feature(5)632..847(+)
Translation
Position Chain Variation Link
9 9 g, t dbSNP:745949381
16 16 c, t dbSNP:757550990
57 57 -, tgc dbSNP:147057701
64 64 a, g dbSNP:3024793
79 79 c, g dbSNP:369145822
90 90 g, t dbSNP:561295206
91 91 c, g dbSNP:763238120
94 94 c, t dbSNP:775711586
108 108 c, t dbSNP:765446107
121 121 c, t dbSNP:779654891
125 125 a, g dbSNP:776744560
131 131 c, t dbSNP:771970362
148 148 a, g dbSNP:377675937
152 152 g, t dbSNP:774179232
158 158 a, c dbSNP:768442204
165 165 g, t dbSNP:749043722
174 174 c, g dbSNP:543297835
180 180 a, g dbSNP:779741450
182 182 c, t dbSNP:755877314
203 203 a, g, t dbSNP:780804657
205 205 a, g dbSNP:756849429
206 206 a, g dbSNP:148914290
207 207 a, g dbSNP:764512527
211 211 c, t dbSNP:557499560
220 220 a, g dbSNP:770763661
233 233 a, g dbSNP:541110574
241 241 c, t dbSNP:145895555
242 242 a, g dbSNP:753519344
244 244 c, t dbSNP:748555153
251 251 c, t dbSNP:761090575
263 263 g, t dbSNP:573709240
288 288 a, c, t dbSNP:45488101
289 289 a, g dbSNP:45478492
291 291 c, t dbSNP:757279120
298 298 g, t dbSNP:751745613
301 301 c, t dbSNP:776398334
303 303 c, t dbSNP:765241667
306 306 a, g dbSNP:755008142
307 307 a, g dbSNP:34682912
308 308 c, g dbSNP:564056875
310 310 a, g dbSNP:766040982
313 313 c, g dbSNP:189048961
319 319 c, t dbSNP:772677222
320 320 a, g dbSNP:767270671
328 328 c, t dbSNP:761303463
341 341 a, g dbSNP:773902434
351 351 a, g dbSNP:768183539
367 367 c, t dbSNP:147417469
368 368 a, g dbSNP:45557339
373 373 c, t dbSNP:368293273
374 374 c, t dbSNP:138729391
377 377 -, aa dbSNP:779795223
377 377 c, g dbSNP:141905538
377 377 c, gaa dbSNP:35328240
388 388 c, t dbSNP:757546697
389 389 a, g dbSNP:140667432
391 391 -, c dbSNP:766821377
392 392 g, t dbSNP:184305471
403 403 c, g dbSNP:758573590
404 404 c, g dbSNP:753771008
408 408 c, t dbSNP:1130866
412 412 c, t dbSNP:529554098
418 418 c, t dbSNP:374131015
419 419 a, g dbSNP:35373464
428 428 a, g dbSNP:751412409
429 429 c, t dbSNP:777381019
433 433 c, t dbSNP:758084893
436 436 a, g dbSNP:752321943
438 438 a, g dbSNP:549877442
452 452 c, t dbSNP:531280852
453 453 a, c, g dbSNP:367831550
475 475 a, g, t dbSNP:773765909
488 488 c, g dbSNP:778541429
490 490 a, c dbSNP:762058470
491 491 c, g, t dbSNP:768712184
495 495 c, t dbSNP:375203471
501 501 a, c dbSNP:781094026
504 504 a, c dbSNP:770903531
509 509 c, t dbSNP:370715446
510 510 a, g dbSNP:370750455
526 526 a, c dbSNP:149904344
532 532 a, c, g dbSNP:377624872
535 535 a, g dbSNP:778434631
536 536 c, g dbSNP:548370069
541 541 c, g dbSNP:754507327
542 542 c, t dbSNP:3024801
544 544 c, t dbSNP:147209702
545 545 a, c, g dbSNP:34550459
547 547 c, t dbSNP:751001412
559 559 a, g dbSNP:767932734
560 560 c, t dbSNP:138274558
562 562 c, t dbSNP:45505791
563 563 a, g dbSNP:184494733
564 564 a, g dbSNP:35524245
566 566 a, c, g dbSNP:778119902
566 566 -, g dbSNP:749997825
576 576 c, t dbSNP:756571077
577 577 a, g dbSNP:543913426
580 580 a, g dbSNP:773147825
585 585 a, g dbSNP:771834627
586 586 c, g dbSNP:141123597
588 588 c, t dbSNP:377545629
592 592 c, t dbSNP:45601634
596 596 c, g dbSNP:748780640
599 599 a, g dbSNP:267599489
607 607 c, t dbSNP:45530632
608 608 a, g dbSNP:747957177
610 610 a, g dbSNP:774056762
613 613 a, g dbSNP:768532112
619 619 a, c dbSNP:190610109
623 623 a, g dbSNP:149956217
627 627 c, g, t dbSNP:150799880
628 628 c, t dbSNP:745426045
631 631 c, t dbSNP:781706067
636 636 a, g dbSNP:757742686
649 649 c, t dbSNP:35049407
665 665 a, c, t dbSNP:758775152
666 666 a, g dbSNP:762917358
678 678 c, t dbSNP:765510340
692 692 g, t dbSNP:753120082
693 693 c, t dbSNP:369801396
694 694 a, g dbSNP:550631097
698 698 c, g dbSNP:2854728
704 704 g, t dbSNP:754123363
710 710 -, g dbSNP:145611216
711 711 c, t dbSNP:766325255
719 719 c, t dbSNP:760826724
722 722 c, t dbSNP:137853202
724 724 c, t dbSNP:367661082
725 725 a, g dbSNP:762818048
728 728 a, g dbSNP:775462975
741 741 a, c, t dbSNP:759213352
742 742 a, g dbSNP:144359413
753 753 a, g dbSNP:770278561
757 757 a, g dbSNP:727503418
760 760 a, c dbSNP:746597889
763 763 a, g dbSNP:773578596
770 770 c, t dbSNP:772619023
771 771 a, g dbSNP:748482999
772 772 a, c dbSNP:779211401
778 778 c, t dbSNP:755360810
779 779 a, g dbSNP:186576601
781 781 a, c, t dbSNP:756130868
790 790 c, g, t dbSNP:764088882
791 791 c, g dbSNP:758367650
795 795 c, t dbSNP:546778802
796 796 a, g dbSNP:528288865
798 798 g, t dbSNP:759379195
802 802 c, g dbSNP:138686998
806 806 c, t dbSNP:45595337
807 807 a, g, t dbSNP:371267944
814 814 g, t dbSNP:771604538
817 817 c, t dbSNP:565870876
831 831 a, g, t dbSNP:3024809
836 836 a, g dbSNP:768946529
842 842 c, t dbSNP:542291993
843 843 a, g dbSNP:201996765
855 855 a, g dbSNP:756396147
859 859 c, t dbSNP:3024810
861 861 g, t dbSNP:45551431
863 863 a, g dbSNP:36210375
876 876 c, t dbSNP:751911024
877 877 a, g dbSNP:747055391
879 879 c, t dbSNP:35076740
880 880 a, g dbSNP:772068983
885 885 a, c, g dbSNP:45504597
891 891 c, g dbSNP:755937427
898 898 a, g dbSNP:750280427
900 900 a, g dbSNP:780787602
904 904 a, c, t dbSNP:538947359
909 909 a, c dbSNP:763598761
913 913 c, t dbSNP:762371851
916 916 c, g dbSNP:571612847
928 928 c, t dbSNP:753242123
929 929 a, g dbSNP:765720471
934 934 c, t dbSNP:546905132
938 938 c, t dbSNP:777177263
944 944 a, g dbSNP:201986026
955 955 c, t dbSNP:529085827
971 971 a, c dbSNP:561113178
976 976 a, g dbSNP:772126727
980 980 c, t dbSNP:527424726
988 988 a, c dbSNP:548414751
1000 1000 c, t dbSNP:775488654
1002 1002 a, g dbSNP:769758832
1018 1018 c, g dbSNP:745832819
1028 1028 a, g dbSNP:561335918
1032 1032 a, g dbSNP:3024813
1085 1085 c, t dbSNP:759743663
1094 1094 a, g dbSNP:560192535
1108 1108 c, t dbSNP:557124359
1135 1135 c, t dbSNP:34757227
1136 1136 a, g dbSNP:35287250
1143 1143 -, gagtcctg dbSNP:560946240
1151 1151 c, t dbSNP:35297133

Target ORF information:

RefSeq Version XM_005264490
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens surfactant protein B (SFTPB), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005264490

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGAGT CACACCTGCT GCAGTGGCTG CTGCTGCTGC TGCCCACGCT CTGTGGCCCA 
GGCACTGCTG CCTGGACCAC CTCATCCTTG GCCTGTGCCC AGGGCCCTGA GTTCTGGTGC
CAAAGCCTGG AGCAAGCATT GCAGTGCAGA GCCCTAGGGC ATTGCCTACA GGAAGTCTGG
GGACATGTGG GAGCCGATGA CCTATGCCAA GAGTGTGAGG ACATCGTCCA CATCCTTAAC
AAGATGGCCA AGGAGGCCAT TTTCCAGGAC ACGATGAGGA AGTTCCTGGA GCAGGAGTGC
AACGTCCTCC CCTTGAAGCT GCTCATGCCC CAGTGCAACC AAGTGCTTGA CGACTACTTC
CCCCTGGTCA TCGACTACTT CCAGAACCAG ACTGACTCAA ACGGCATCTG TATGCACCTG
GGCCTGTGCA AATCCCGGCA GCCAGAGCCA GAGCAGGAGC CAGGGATGTC AGACCCCCTG
CCCAAACCTC TGCGGGACCC TCTGCCAGAC CCTCTGCTGG ACAAGCTCGT CCTCCCTGTG
CTGCCCGGGG CCCTCCAGGC GAGGCCTGGG CCTCACACAC AGGATCTCTC CGAGCAGCAA
TTCCCCATTC CTCTCCCCTA TTGCTGGCTC TGCAGGGCTC TGATCAAGCG GATCCAAGCC
ATGATTCCCA AGGGTGCGCT AGCTGTGGCA GTGGCCCAGG TGTGCCGCGT GGTACCTCTG
GTGGCGGGCG GCATCTGCCA GTGCCTGGCT GAGCGCTACT CCGTCATCCT GCTCGACACG
CTGCTGGGCC GCATGCTGCC CCAGCTGGTC TGCCGCCTCG TCCTCCGGTG CTCCATGGAT
GACAGCGCTG GCCCAAGGTC GCCGACAGGA GAATGGCTGC CGCGAGACTC TGAGTGCCAC
CTCTGCATGT CCGTGACCAC CCAGGCCGGG AACAGCAGCG AGCAGGCCAT ACCACAGGCA
ATGCTCCAGG CCTGTGTTGG CTCCTGGCTG GACAGGGAAA AGCTGGCTCC AGTTTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09313
Accession Version NM_198843.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pulmonary surfactant-associated protein B precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL548140.3, BC032785.1, AB439563.1, CA437348.1 and AW204600.1. This sequence is a reference standard in the RefSeqGene project. On Feb 13, 2010 this sequence version replaced gi:38504666. Summary: This gene encodes the pulmonary-associated surfactant protein B (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period. Alternatively spliced transcript variants encoding the same protein have been identified.[provided by RefSeq, Feb 2010]. Transcript Variant: This variant (2) lacks an internal segment in the 3' UTR, as compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2147920, SAMEA2155770 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACCAAG CAGGGTACCC AGGCTGCAGA GGTGCCATGG CTGAGTCACA CCTGCTGCAG 
TGGCTGCTGC TGCTGCTGCC CACGCTCTGT GGCCCAGGCA CTGCTGCCTG GACCACCTCA
TCCTTGGCCT GTGCCCAGGG CCCTGAGTTC TGGTGCCAAA GCCTGGAGCA AGCATTGCAG
TGCAGAGCCC TAGGGCATTG CCTACAGGAA GTCTGGGGAC ATGTGGGAGC CGATGACCTA
TGCCAAGAGT GTGAGGACAT CGTCCACATC CTTAACAAGA TGGCCAAGGA GGCCATTTTC
CAGGACACGA TGAGGAAGTT CCTGGAGCAG GAGTGCAACG TCCTCCCCTT GAAGCTGCTC
ATGCCCCAGT GCAACCAAGT GCTTGACGAC TACTTCCCCC TGGTCATCGA CTACTTCCAG
AACCAGACTG ACTCAAACGG CATCTGTATG CACCTGGGCC TGTGCAAATC CCGGCAGCCA
GAGCCAGAGC AGGAGCCAGG GATGTCAGAC CCCCTGCCCA AACCTCTGCG GGACCCTCTG
CCAGACCCTC TGCTGGACAA GCTCGTCCTC CCTGTGCTGC CCGGGGCCCT CCAGGCGAGG
CCTGGGCCTC ACACACAGGA TCTCTCCGAG CAGCAATTCC CCATTCCTCT CCCCTATTGC
TGGCTCTGCA GGGCTCTGAT CAAGCGGATC CAAGCCATGA TTCCCAAGGG TGCGCTAGCT
GTGGCAGTGG CCCAGGTGTG CCGCGTGGTA CCTCTGGTGG CGGGCGGCAT CTGCCAGTGC
CTGGCTGAGC GCTACTCCGT CATCCTGCTC GACACGCTGC TGGGCCGCAT GCTGCCCCAG
CTGGTCTGCC GCCTCGTCCT CCGGTGCTCC ATGGATGACA GCGCTGGCCC AAGGTCGCCG
ACAGGAGAAT GGCTGCCGCG AGACTCTGAG TGCCACCTCT GCATGTCCGT GACCACCCAG
GCCGGGAACA GCAGCGAGCA GGCCATACCA CAGGCAATGC TCCAGGCCTG TGTTGGCTCC
TGGCTGGACA GGGAAAAGTG CAAGCAATTT GTGGAGCAGC ACACGCCCCA GCTGCTGACC
CTGGTGCCCA GGGGCTGGGA TGCCCACACC ACCTGCCAGG CCCTCGGGGT GTGTGGGACC
ATGTCCAGCC CTCTCCAGTG TATCCACAGC CCCGACCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_942140.2
CDS101..1282
Misc Feature(1)92..94(+)
Misc Feature(2)218..319(+)
Misc Feature(3)332..448(+)
Misc Feature(4)335..565(+)
Misc Feature(5)464..559(+)
Misc Feature(6)752..967(+)
Misc Feature(7)1025..1234(+)
Misc Feature(8)1130..1228(+)
Exon (1)1..68
Gene:SFTPB
Gene Synonym:
Exon (2)69..203
Gene:SFTPB
Gene Synonym:
Exon (3)204..331
Gene:SFTPB
Gene Synonym:
Exon (4)332..403
Gene:SFTPB
Gene Synonym:
Exon (5)404..529
Gene:SFTPB
Gene Synonym:
Exon (6)530..718
Gene:SFTPB
Gene Synonym:
Exon (7)719..808
Gene:SFTPB
Gene Synonym:
Exon (8)809..992
Gene:SFTPB
Gene Synonym:
Exon (9)993..1138
Gene:SFTPB
Gene Synonym:
Exon (10)1139..1219
Gene:SFTPB
Gene Synonym:
Exon (11)1220..1301
Gene:SFTPB
Gene Synonym:
Exon (12)1302..2840
Gene:SFTPB
Gene Synonym:
Translation
Position Chain Variation Link
4 4 a, c dbSNP:530213624
28 28 c, g dbSNP:562887288
47 47 a, g dbSNP:762114371
77 77 c, t dbSNP:546582871
78 78 a, g dbSNP:762253717
82 82 c, t dbSNP:534925921
83 83 a, g dbSNP:34024265
86 86 a, c dbSNP:768729156
95 95 c, t dbSNP:3024792
97 97 a, g dbSNP:763190494
99 99 c, t dbSNP:35531973
105 105 a, c dbSNP:2077079
106 106 c, t dbSNP:35509268
129 129 g, t dbSNP:745949381
136 136 c, t dbSNP:757550990
177 177 -, tgc dbSNP:147057701
184 184 a, g dbSNP:3024793
199 199 c, g dbSNP:369145822
210 210 g, t dbSNP:561295206
211 211 c, g dbSNP:763238120
214 214 c, t dbSNP:775711586
228 228 c, t dbSNP:765446107
241 241 c, t dbSNP:779654891
245 245 a, g dbSNP:776744560
251 251 c, t dbSNP:771970362
268 268 a, g dbSNP:377675937
272 272 g, t dbSNP:774179232
278 278 a, c dbSNP:768442204
285 285 g, t dbSNP:749043722
294 294 c, g dbSNP:543297835
300 300 a, g dbSNP:779741450
302 302 c, t dbSNP:755877314
323 323 a, g, t dbSNP:780804657
325 325 a, g dbSNP:756849429
326 326 a, g dbSNP:148914290
327 327 a, g dbSNP:764512527
331 331 c, t dbSNP:557499560
340 340 a, g dbSNP:770763661
353 353 a, g dbSNP:541110574
361 361 c, t dbSNP:145895555
362 362 a, g dbSNP:753519344
364 364 c, t dbSNP:748555153
371 371 c, t dbSNP:761090575
383 383 g, t dbSNP:573709240
408 408 a, c, t dbSNP:45488101
409 409 a, g dbSNP:45478492
411 411 c, t dbSNP:757279120
418 418 g, t dbSNP:751745613
421 421 c, t dbSNP:776398334
423 423 c, t dbSNP:765241667
426 426 a, g dbSNP:755008142
427 427 a, g dbSNP:34682912
428 428 c, g dbSNP:564056875
430 430 a, g dbSNP:766040982
433 433 c, g dbSNP:189048961
439 439 c, t dbSNP:772677222
440 440 a, g dbSNP:767270671
448 448 c, t dbSNP:761303463
461 461 a, g dbSNP:773902434
471 471 a, g dbSNP:768183539
487 487 c, t dbSNP:147417469
488 488 a, g dbSNP:45557339
493 493 c, t dbSNP:368293273
494 494 c, t dbSNP:138729391
497 497 -, aa dbSNP:779795223
497 497 c, g dbSNP:141905538
497 497 c, gaa dbSNP:35328240
508 508 c, t dbSNP:757546697
509 509 a, g dbSNP:140667432
511 511 -, c dbSNP:766821377
512 512 g, t dbSNP:184305471
523 523 c, g dbSNP:758573590
524 524 c, g dbSNP:753771008
528 528 c, t dbSNP:1130866
532 532 c, t dbSNP:529554098
538 538 c, t dbSNP:374131015
539 539 a, g dbSNP:35373464
548 548 a, g dbSNP:751412409
549 549 c, t dbSNP:777381019
553 553 c, t dbSNP:758084893
556 556 a, g dbSNP:752321943
558 558 a, g dbSNP:549877442
572 572 c, t dbSNP:531280852
573 573 a, c, g dbSNP:367831550
595 595 a, g, t dbSNP:773765909
608 608 c, g dbSNP:778541429
610 610 a, c dbSNP:762058470
611 611 c, g, t dbSNP:768712184
615 615 c, t dbSNP:375203471
621 621 a, c dbSNP:781094026
624 624 a, c dbSNP:770903531
629 629 c, t dbSNP:370715446
630 630 a, g dbSNP:370750455
646 646 a, c dbSNP:149904344
652 652 a, c, g dbSNP:377624872
655 655 a, g dbSNP:778434631
656 656 c, g dbSNP:548370069
661 661 c, g dbSNP:754507327
662 662 c, t dbSNP:3024801
664 664 c, t dbSNP:147209702
665 665 a, c, g dbSNP:34550459
667 667 c, t dbSNP:751001412
679 679 a, g dbSNP:767932734
680 680 c, t dbSNP:138274558
682 682 c, t dbSNP:45505791
683 683 a, g dbSNP:184494733
684 684 a, g dbSNP:35524245
686 686 a, c, g dbSNP:778119902
686 686 -, g dbSNP:749997825
696 696 c, t dbSNP:756571077
697 697 a, g dbSNP:543913426
700 700 a, g dbSNP:773147825
705 705 a, g dbSNP:771834627
706 706 c, g dbSNP:141123597
708 708 c, t dbSNP:377545629
712 712 c, t dbSNP:45601634
716 716 c, g dbSNP:748780640
719 719 a, g dbSNP:267599489
727 727 c, t dbSNP:45530632
728 728 a, g dbSNP:747957177
730 730 a, g dbSNP:774056762
733 733 a, g dbSNP:768532112
739 739 a, c dbSNP:190610109
743 743 a, g dbSNP:149956217
747 747 c, g, t dbSNP:150799880
748 748 c, t dbSNP:745426045
751 751 c, t dbSNP:781706067
756 756 a, g dbSNP:757742686
769 769 c, t dbSNP:35049407
785 785 a, c, t dbSNP:758775152
786 786 a, g dbSNP:762917358
798 798 c, t dbSNP:765510340
812 812 g, t dbSNP:753120082
813 813 c, t dbSNP:369801396
814 814 a, g dbSNP:550631097
818 818 c, g dbSNP:2854728
824 824 g, t dbSNP:754123363
830 830 -, g dbSNP:145611216
831 831 c, t dbSNP:766325255
839 839 c, t dbSNP:760826724
842 842 c, t dbSNP:137853202
844 844 c, t dbSNP:367661082
845 845 a, g dbSNP:762818048
848 848 a, g dbSNP:775462975
861 861 a, c, t dbSNP:759213352
862 862 a, g dbSNP:144359413
873 873 a, g dbSNP:770278561
877 877 a, g dbSNP:727503418
880 880 a, c dbSNP:746597889
883 883 a, g dbSNP:773578596
890 890 c, t dbSNP:772619023
891 891 a, g dbSNP:748482999
892 892 a, c dbSNP:779211401
898 898 c, t dbSNP:755360810
899 899 a, g dbSNP:186576601
901 901 a, c, t dbSNP:756130868
910 910 c, g, t dbSNP:764088882
911 911 c, g dbSNP:758367650
915 915 c, t dbSNP:546778802
916 916 a, g dbSNP:528288865
918 918 g, t dbSNP:759379195
922 922 c, g dbSNP:138686998
926 926 c, t dbSNP:45595337
927 927 a, g, t dbSNP:371267944
934 934 g, t dbSNP:771604538
937 937 c, t dbSNP:565870876
951 951 a, g, t dbSNP:3024809
956 956 a, g dbSNP:768946529
962 962 c, t dbSNP:542291993
963 963 a, g dbSNP:201996765
975 975 a, g dbSNP:756396147
979 979 c, t dbSNP:3024810
981 981 g, t dbSNP:45551431
983 983 a, g dbSNP:36210375
996 996 c, t dbSNP:751911024
997 997 a, g dbSNP:747055391
999 999 c, t dbSNP:35076740
1000 1000 a, g dbSNP:772068983
1005 1005 a, c, g dbSNP:45504597
1011 1011 c, g dbSNP:755937427
1018 1018 a, g dbSNP:750280427
1020 1020 a, g dbSNP:780787602
1024 1024 a, c, t dbSNP:538947359
1029 1029 a, c dbSNP:763598761
1033 1033 c, t dbSNP:762371851
1036 1036 c, g dbSNP:571612847
1048 1048 c, t dbSNP:753242123
1049 1049 a, g dbSNP:765720471
1054 1054 c, t dbSNP:546905132
1058 1058 c, t dbSNP:777177263
1064 1064 a, g dbSNP:201986026
1075 1075 c, t dbSNP:529085827
1091 1091 a, c dbSNP:561113178
1096 1096 a, g dbSNP:772126727
1100 1100 c, t dbSNP:527424726
1108 1108 a, c dbSNP:548414751
1120 1120 c, t dbSNP:775488654
1122 1122 a, g dbSNP:769758832
1138 1138 c, g dbSNP:745832819
1140 1140 a, g dbSNP:767694183
1146 1146 a, t dbSNP:143687195
1164 1164 c, t dbSNP:567506649
1165 1165 a, g dbSNP:45606043
1166 1166 a, c dbSNP:745744765
1167 1167 -, gcc dbSNP:781302871
1177 1177 a, c, g dbSNP:368681670
1182 1182 g, t dbSNP:746836305
1186 1186 a, g dbSNP:777459025
1220 1220 g, t dbSNP:780688071
1225 1225 c, t dbSNP:756623575
1226 1226 a, g dbSNP:750958534
1231 1231 a, g dbSNP:781349851
1233 1233 g, t dbSNP:757642887
1245 1245 c, t dbSNP:374932425
1249 1249 a, c dbSNP:370528656
1251 1251 c, g dbSNP:368016212
1254 1254 -, tc dbSNP:751886630
1258 1258 c, g dbSNP:551956264
1272 1272 c, g dbSNP:144831319
1274 1274 a, g dbSNP:370785493
1285 1285 a, t dbSNP:773204473
1291 1291 c, t dbSNP:771704983
1294 1294 a, c dbSNP:45529633
1297 1297 c, t dbSNP:371255380
1300 1300 a, g dbSNP:774139517
1301 1301 g, t dbSNP:768460155
1312 1312 c, t dbSNP:3024828
1356 1356 c, t dbSNP:754426598
1376 1376 g, t dbSNP:763552176
1383 1383 a, g dbSNP:566440917
1388 1388 a, g dbSNP:548209846
1400 1400 g, t dbSNP:529965924
1416 1416 c, g dbSNP:562683337
1589 1589 c, t dbSNP:760210420
1632 1632 a, t dbSNP:568399684
1644 1644 a, c dbSNP:114238614
1661 1661 c, t dbSNP:775883772
1696 1696 a, g dbSNP:375619953
1735 1735 c, g dbSNP:532344629
1775 1775 c, t dbSNP:768029277
1802 1802 c, t dbSNP:182172598
1818 1818 a, g dbSNP:191054350
1871 1871 a, g dbSNP:759899702
1878 1878 c, t dbSNP:572380293
1940 1940 c, g dbSNP:774641444
1943 1943 a, g dbSNP:560513740
1953 1953 c, t dbSNP:542165602
1957 1957 c, t dbSNP:575039299
1981 1981 c, t dbSNP:56204952
2013 2013 c, t dbSNP:773456371
2046 2046 a, c dbSNP:556431838
2095 2095 a, t dbSNP:186308421
2150 2150 c, t dbSNP:113189011
2185 2185 c, t dbSNP:549991026
2190 2190 c, t dbSNP:138799416
2218 2218 -, agaat dbSNP:374853987
2238 2238 c, t dbSNP:748215074
2247 2247 c, t dbSNP:10625
2260 2260 a, g dbSNP:558265142
2276 2276 a, g dbSNP:1065192
2302 2302 c, t dbSNP:115696450
2303 2303 a, g dbSNP:3024829
2312 2312 a, t dbSNP:745784912
2326 2326 g, t dbSNP:554501590
2332 2332 a, c dbSNP:536163759
2356 2356 c, t dbSNP:531786266
2367 2367 a, g dbSNP:113824447
2373 2373 a, c dbSNP:532373831
2396 2396 c, t dbSNP:371563274
2414 2414 c, t dbSNP:753594106
2499 2499 a, t dbSNP:564463967
2554 2554 a, g dbSNP:201660047
2597 2597 c, t dbSNP:200871431
2598 2598 a, c dbSNP:546289871
2636 2636 a, g dbSNP:3024830
2691 2691 c, t dbSNP:552403996
2695 2695 c, t dbSNP:752187313
2794 2794 a, g dbSNP:527741702
2803 2803 a, t dbSNP:56192916

Target ORF information:

RefSeq Version NM_198843
Organism Homo sapiens (human)
Definition Homo sapiens surfactant protein B (SFTPB), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_198843

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACCAAG CAGGGTACCC AGGCTGCAGA GGTGCCATGG CTGAGTCACA CCTGCTGCAG 
TGGCTGCTGC TGCTGCTGCC CACGCTCTGT GGCCCAGGCA CTGCTGCCTG GACCACCTCA
TCCTTGGCCT GTGCCCAGGG CCCTGAGTTC TGGTGCCAAA GCCTGGAGCA AGCATTGCAG
TGCAGAGCCC TAGGGCATTG CCTACAGGAA GTCTGGGGAC ATGTGGGAGC CGATGACCTA
TGCCAAGAGT GTGAGGACAT CGTCCACATC CTTAACAAGA TGGCCAAGGA GGCCATTTTC
CAGGACACGA TGAGGAAGTT CCTGGAGCAG GAGTGCAACG TCCTCCCCTT GAAGCTGCTC
ATGCCCCAGT GCAACCAAGT GCTTGACGAC TACTTCCCCC TGGTCATCGA CTACTTCCAG
AACCAGACTG ACTCAAACGG CATCTGTATG CACCTGGGCC TGTGCAAATC CCGGCAGCCA
GAGCCAGAGC AGGAGCCAGG GATGTCAGAC CCCCTGCCCA AACCTCTGCG GGACCCTCTG
CCAGACCCTC TGCTGGACAA GCTCGTCCTC CCTGTGCTGC CCGGGGCCCT CCAGGCGAGG
CCTGGGCCTC ACACACAGGA TCTCTCCGAG CAGCAATTCC CCATTCCTCT CCCCTATTGC
TGGCTCTGCA GGGCTCTGAT CAAGCGGATC CAAGCCATGA TTCCCAAGGG TGCGCTAGCT
GTGGCAGTGG CCCAGGTGTG CCGCGTGGTA CCTCTGGTGG CGGGCGGCAT CTGCCAGTGC
CTGGCTGAGC GCTACTCCGT CATCCTGCTC GACACGCTGC TGGGCCGCAT GCTGCCCCAG
CTGGTCTGCC GCCTCGTCCT CCGGTGCTCC ATGGATGACA GCGCTGGCCC AAGGTCGCCG
ACAGGAGAAT GGCTGCCGCG AGACTCTGAG TGCCACCTCT GCATGTCCGT GACCACCCAG
GCCGGGAACA GCAGCGAGCA GGCCATACCA CAGGCAATGC TCCAGGCCTG TGTTGGCTCC
TGGCTGGACA GGGAAAAGTG CAAGCAATTT GTGGAGCAGC ACACGCCCCA GCTGCTGACC
CTGGTGCCCA GGGGCTGGGA TGCCCACACC ACCTGCCAGG CCCTCGGGGT GTGTGGGACC
ATGTCCAGCC CTCTCCAGTG TATCCACAGC CCCGACCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09313
Accession Version NM_000542.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pulmonary surfactant-associated protein B precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL548140.3, BC032785.1, BG482852.1, AB439563.1, CA437348.1 and AW204600.1. On Feb 13, 2010 this sequence version replaced gi:33943098. Summary: This gene encodes the pulmonary-associated surfactant protein B (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period. Alternatively spliced transcript variants encoding the same protein have been identified.[provided by RefSeq, Feb 2010]. Transcript Variant: This variant (1) is the longer transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2147920, SAMEA2155770 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACCAAG CAGGGTACCC AGGCTGCAGA GGTGCCATGG CTGAGTCACA CCTGCTGCAG 
TGGCTGCTGC TGCTGCTGCC CACGCTCTGT GGCCCAGGCA CTGCTGCCTG GACCACCTCA
TCCTTGGCCT GTGCCCAGGG CCCTGAGTTC TGGTGCCAAA GCCTGGAGCA AGCATTGCAG
TGCAGAGCCC TAGGGCATTG CCTACAGGAA GTCTGGGGAC ATGTGGGAGC CGATGACCTA
TGCCAAGAGT GTGAGGACAT CGTCCACATC CTTAACAAGA TGGCCAAGGA GGCCATTTTC
CAGGACACGA TGAGGAAGTT CCTGGAGCAG GAGTGCAACG TCCTCCCCTT GAAGCTGCTC
ATGCCCCAGT GCAACCAAGT GCTTGACGAC TACTTCCCCC TGGTCATCGA CTACTTCCAG
AACCAGACTG ACTCAAACGG CATCTGTATG CACCTGGGCC TGTGCAAATC CCGGCAGCCA
GAGCCAGAGC AGGAGCCAGG GATGTCAGAC CCCCTGCCCA AACCTCTGCG GGACCCTCTG
CCAGACCCTC TGCTGGACAA GCTCGTCCTC CCTGTGCTGC CCGGGGCCCT CCAGGCGAGG
CCTGGGCCTC ACACACAGGA TCTCTCCGAG CAGCAATTCC CCATTCCTCT CCCCTATTGC
TGGCTCTGCA GGGCTCTGAT CAAGCGGATC CAAGCCATGA TTCCCAAGGG TGCGCTAGCT
GTGGCAGTGG CCCAGGTGTG CCGCGTGGTA CCTCTGGTGG CGGGCGGCAT CTGCCAGTGC
CTGGCTGAGC GCTACTCCGT CATCCTGCTC GACACGCTGC TGGGCCGCAT GCTGCCCCAG
CTGGTCTGCC GCCTCGTCCT CCGGTGCTCC ATGGATGACA GCGCTGGCCC AAGGTCGCCG
ACAGGAGAAT GGCTGCCGCG AGACTCTGAG TGCCACCTCT GCATGTCCGT GACCACCCAG
GCCGGGAACA GCAGCGAGCA GGCCATACCA CAGGCAATGC TCCAGGCCTG TGTTGGCTCC
TGGCTGGACA GGGAAAAGTG CAAGCAATTT GTGGAGCAGC ACACGCCCCA GCTGCTGACC
CTGGTGCCCA GGGGCTGGGA TGCCCACACC ACCTGCCAGG CCCTCGGGGT GTGTGGGACC
ATGTCCAGCC CTCTCCAGTG TATCCACAGC CCCGACCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_000533.3
CDS101..1282
Misc Feature(1)92..94(+)
Misc Feature(2)218..319(+)
Misc Feature(3)332..448(+)
Misc Feature(4)335..565(+)
Misc Feature(5)464..559(+)
Misc Feature(6)752..967(+)
Misc Feature(7)1025..1234(+)
Misc Feature(8)1130..1228(+)
Exon (1)1..68
Gene:SFTPB
Gene Synonym:
Exon (2)69..203
Gene:SFTPB
Gene Synonym:
Exon (3)204..331
Gene:SFTPB
Gene Synonym:
Exon (4)332..403
Gene:SFTPB
Gene Synonym:
Exon (5)404..529
Gene:SFTPB
Gene Synonym:
Exon (6)530..718
Gene:SFTPB
Gene Synonym:
Exon (7)719..808
Gene:SFTPB
Gene Synonym:
Exon (8)809..992
Gene:SFTPB
Gene Synonym:
Exon (9)993..1138
Gene:SFTPB
Gene Synonym:
Exon (10)1139..1219
Gene:SFTPB
Gene Synonym:
Exon (11)1220..1301
Gene:SFTPB
Gene Synonym:
Exon (12)1302..3667
Gene:SFTPB
Gene Synonym:
Translation
Position Chain Variation Link
4 4 a, c dbSNP:530213624
28 28 c, g dbSNP:562887288
47 47 a, g dbSNP:762114371
77 77 c, t dbSNP:546582871
78 78 a, g dbSNP:762253717
82 82 c, t dbSNP:534925921
83 83 a, g dbSNP:34024265
86 86 a, c dbSNP:768729156
95 95 c, t dbSNP:3024792
97 97 a, g dbSNP:763190494
99 99 c, t dbSNP:35531973
105 105 a, c dbSNP:2077079
106 106 c, t dbSNP:35509268
129 129 g, t dbSNP:745949381
136 136 c, t dbSNP:757550990
177 177 -, tgc dbSNP:147057701
184 184 a, g dbSNP:3024793
199 199 c, g dbSNP:369145822
210 210 g, t dbSNP:561295206
211 211 c, g dbSNP:763238120
214 214 c, t dbSNP:775711586
228 228 c, t dbSNP:765446107
241 241 c, t dbSNP:779654891
245 245 a, g dbSNP:776744560
251 251 c, t dbSNP:771970362
268 268 a, g dbSNP:377675937
272 272 g, t dbSNP:774179232
278 278 a, c dbSNP:768442204
285 285 g, t dbSNP:749043722
294 294 c, g dbSNP:543297835
300 300 a, g dbSNP:779741450
302 302 c, t dbSNP:755877314
323 323 a, g, t dbSNP:780804657
325 325 a, g dbSNP:756849429
326 326 a, g dbSNP:148914290
327 327 a, g dbSNP:764512527
331 331 c, t dbSNP:557499560
340 340 a, g dbSNP:770763661
353 353 a, g dbSNP:541110574
361 361 c, t dbSNP:145895555
362 362 a, g dbSNP:753519344
364 364 c, t dbSNP:748555153
371 371 c, t dbSNP:761090575
383 383 g, t dbSNP:573709240
408 408 a, c, t dbSNP:45488101
409 409 a, g dbSNP:45478492
411 411 c, t dbSNP:757279120
418 418 g, t dbSNP:751745613
421 421 c, t dbSNP:776398334
423 423 c, t dbSNP:765241667
426 426 a, g dbSNP:755008142
427 427 a, g dbSNP:34682912
428 428 c, g dbSNP:564056875
430 430 a, g dbSNP:766040982
433 433 c, g dbSNP:189048961
439 439 c, t dbSNP:772677222
440 440 a, g dbSNP:767270671
448 448 c, t dbSNP:761303463
461 461 a, g dbSNP:773902434
471 471 a, g dbSNP:768183539
487 487 c, t dbSNP:147417469
488 488 a, g dbSNP:45557339
493 493 c, t dbSNP:368293273
494 494 c, t dbSNP:138729391
497 497 -, aa dbSNP:779795223
497 497 c, g dbSNP:141905538
497 497 c, gaa dbSNP:35328240
508 508 c, t dbSNP:757546697
509 509 a, g dbSNP:140667432
511 511 -, c dbSNP:766821377
512 512 g, t dbSNP:184305471
523 523 c, g dbSNP:758573590
524 524 c, g dbSNP:753771008
528 528 c, t dbSNP:1130866
532 532 c, t dbSNP:529554098
538 538 c, t dbSNP:374131015
539 539 a, g dbSNP:35373464
548 548 a, g dbSNP:751412409
549 549 c, t dbSNP:777381019
553 553 c, t dbSNP:758084893
556 556 a, g dbSNP:752321943
558 558 a, g dbSNP:549877442
572 572 c, t dbSNP:531280852
573 573 a, c, g dbSNP:367831550
595 595 a, g, t dbSNP:773765909
608 608 c, g dbSNP:778541429
610 610 a, c dbSNP:762058470
611 611 c, g, t dbSNP:768712184
615 615 c, t dbSNP:375203471
621 621 a, c dbSNP:781094026
624 624 a, c dbSNP:770903531
629 629 c, t dbSNP:370715446
630 630 a, g dbSNP:370750455
646 646 a, c dbSNP:149904344
652 652 a, c, g dbSNP:377624872
655 655 a, g dbSNP:778434631
656 656 c, g dbSNP:548370069
661 661 c, g dbSNP:754507327
662 662 c, t dbSNP:3024801
664 664 c, t dbSNP:147209702
665 665 a, c, g dbSNP:34550459
667 667 c, t dbSNP:751001412
679 679 a, g dbSNP:767932734
680 680 c, t dbSNP:138274558
682 682 c, t dbSNP:45505791
683 683 a, g dbSNP:184494733
684 684 a, g dbSNP:35524245
686 686 a, c, g dbSNP:778119902
686 686 -, g dbSNP:749997825
696 696 c, t dbSNP:756571077
697 697 a, g dbSNP:543913426
700 700 a, g dbSNP:773147825
705 705 a, g dbSNP:771834627
706 706 c, g dbSNP:141123597
708 708 c, t dbSNP:377545629
712 712 c, t dbSNP:45601634
716 716 c, g dbSNP:748780640
719 719 a, g dbSNP:267599489
727 727 c, t dbSNP:45530632
728 728 a, g dbSNP:747957177
730 730 a, g dbSNP:774056762
733 733 a, g dbSNP:768532112
739 739 a, c dbSNP:190610109
743 743 a, g dbSNP:149956217
747 747 c, g, t dbSNP:150799880
748 748 c, t dbSNP:745426045
751 751 c, t dbSNP:781706067
756 756 a, g dbSNP:757742686
769 769 c, t dbSNP:35049407
785 785 a, c, t dbSNP:758775152
786 786 a, g dbSNP:762917358
798 798 c, t dbSNP:765510340
812 812 g, t dbSNP:753120082
813 813 c, t dbSNP:369801396
814 814 a, g dbSNP:550631097
818 818 c, g dbSNP:2854728
824 824 g, t dbSNP:754123363
830 830 -, g dbSNP:145611216
831 831 c, t dbSNP:766325255
839 839 c, t dbSNP:760826724
842 842 c, t dbSNP:137853202
844 844 c, t dbSNP:367661082
845 845 a, g dbSNP:762818048
848 848 a, g dbSNP:775462975
861 861 a, c, t dbSNP:759213352
862 862 a, g dbSNP:144359413
873 873 a, g dbSNP:770278561
877 877 a, g dbSNP:727503418
880 880 a, c dbSNP:746597889
883 883 a, g dbSNP:773578596
890 890 c, t dbSNP:772619023
891 891 a, g dbSNP:748482999
892 892 a, c dbSNP:779211401
898 898 c, t dbSNP:755360810
899 899 a, g dbSNP:186576601
901 901 a, c, t dbSNP:756130868
910 910 c, g, t dbSNP:764088882
911 911 c, g dbSNP:758367650
915 915 c, t dbSNP:546778802
916 916 a, g dbSNP:528288865
918 918 g, t dbSNP:759379195
922 922 c, g dbSNP:138686998
926 926 c, t dbSNP:45595337
927 927 a, g, t dbSNP:371267944
934 934 g, t dbSNP:771604538
937 937 c, t dbSNP:565870876
951 951 a, g, t dbSNP:3024809
956 956 a, g dbSNP:768946529
962 962 c, t dbSNP:542291993
963 963 a, g dbSNP:201996765
975 975 a, g dbSNP:756396147
979 979 c, t dbSNP:3024810
981 981 g, t dbSNP:45551431
983 983 a, g dbSNP:36210375
996 996 c, t dbSNP:751911024
997 997 a, g dbSNP:747055391
999 999 c, t dbSNP:35076740
1000 1000 a, g dbSNP:772068983
1005 1005 a, c, g dbSNP:45504597
1011 1011 c, g dbSNP:755937427
1018 1018 a, g dbSNP:750280427
1020 1020 a, g dbSNP:780787602
1024 1024 a, c, t dbSNP:538947359
1029 1029 a, c dbSNP:763598761
1033 1033 c, t dbSNP:762371851
1036 1036 c, g dbSNP:571612847
1048 1048 c, t dbSNP:753242123
1049 1049 a, g dbSNP:765720471
1054 1054 c, t dbSNP:546905132
1058 1058 c, t dbSNP:777177263
1064 1064 a, g dbSNP:201986026
1075 1075 c, t dbSNP:529085827
1091 1091 a, c dbSNP:561113178
1096 1096 a, g dbSNP:772126727
1100 1100 c, t dbSNP:527424726
1108 1108 a, c dbSNP:548414751
1120 1120 c, t dbSNP:775488654
1122 1122 a, g dbSNP:769758832
1138 1138 c, g dbSNP:745832819
1140 1140 a, g dbSNP:767694183
1146 1146 a, t dbSNP:143687195
1164 1164 c, t dbSNP:567506649
1165 1165 a, g dbSNP:45606043
1166 1166 a, c dbSNP:745744765
1167 1167 -, gcc dbSNP:781302871
1177 1177 a, c, g dbSNP:368681670
1182 1182 g, t dbSNP:746836305
1186 1186 a, g dbSNP:777459025
1220 1220 g, t dbSNP:780688071
1225 1225 c, t dbSNP:756623575
1226 1226 a, g dbSNP:750958534
1231 1231 a, g dbSNP:781349851
1233 1233 g, t dbSNP:757642887
1245 1245 c, t dbSNP:374932425
1249 1249 a, c dbSNP:370528656
1251 1251 c, g dbSNP:368016212
1254 1254 -, tc dbSNP:751886630
1258 1258 c, g dbSNP:551956264
1272 1272 c, g dbSNP:144831319
1274 1274 a, g dbSNP:370785493
1285 1285 a, t dbSNP:773204473
1291 1291 c, t dbSNP:771704983
1294 1294 a, c dbSNP:45529633
1297 1297 c, t dbSNP:371255380
1300 1300 a, g dbSNP:774139517
1301 1301 g, t dbSNP:768460155
1327 1327 a, g dbSNP:555613505
1332 1332 c, g dbSNP:369263105
1367 1367 a, c dbSNP:56811788
1376 1376 a, g dbSNP:543912566
1416 1416 a, g dbSNP:576528370
1428 1428 g, t dbSNP:1804246
1438 1438 a, g dbSNP:114691084
1440 1440 c, g dbSNP:9752
1454 1454 a, g dbSNP:566196796
1463 1463 c, t dbSNP:555632775
1517 1517 a, g dbSNP:3024822
1518 1518 c, t dbSNP:568626838
1535 1535 c, t dbSNP:549483539
1538 1538 c, t dbSNP:188417591
1600 1600 c, t dbSNP:570119766
1608 1608 c, t dbSNP:551707788
1633 1633 c, t dbSNP:3024823
1654 1654 a, g dbSNP:560227586
1669 1669 c, t dbSNP:745310719
1670 1670 c, t dbSNP:545321542
1718 1718 c, t dbSNP:749387121
1727 1727 a, t dbSNP:569810989
1760 1760 a, g dbSNP:578093456
1762 1762 a, g dbSNP:182975929
1765 1765 c, t dbSNP:3024824
1773 1773 -, taga dbSNP:369576391
1800 1800 g, t dbSNP:191130863
1814 1814 a, g dbSNP:562675679
1828 1828 a, g dbSNP:185386014
1836 1836 g, t dbSNP:148430793
1837 1837 a, g dbSNP:571402858
1850 1850 c, t dbSNP:756851833
1875 1875 c, t dbSNP:564476672
1876 1876 a, g dbSNP:144475205
1877 1877 a, g dbSNP:572397585
1886 1886 g, t dbSNP:554205820
1894 1894 a, g dbSNP:367597303
1899 1899 a, g dbSNP:779988988
1931 1931 -, atg dbSNP:3024825
1931 1931 cat, g dbSNP:367798135
1931 1931 c, g dbSNP:574947393
1936 1936 -, ta dbSNP:147067432
1942 1942 c, g dbSNP:3024826
1966 1966 a, g dbSNP:758155280
1987 1987 g, t dbSNP:537548634
2000 2000 a, g dbSNP:367966703
2038 2038 c, g dbSNP:3024827
2042 2042 -, g dbSNP:35502847
2046 2046 a, g dbSNP:764992096
2093 2093 a, g dbSNP:11544469
2094 2094 a, g dbSNP:7316
2139 2139 c, t dbSNP:3024828
2183 2183 c, t dbSNP:754426598
2203 2203 g, t dbSNP:763552176
2210 2210 a, g dbSNP:566440917
2215 2215 a, g dbSNP:548209846
2227 2227 g, t dbSNP:529965924
2243 2243 c, g dbSNP:562683337
2416 2416 c, t dbSNP:760210420
2459 2459 a, t dbSNP:568399684
2471 2471 a, c dbSNP:114238614
2488 2488 c, t dbSNP:775883772
2523 2523 a, g dbSNP:375619953
2562 2562 c, g dbSNP:532344629
2602 2602 c, t dbSNP:768029277
2629 2629 c, t dbSNP:182172598
2645 2645 a, g dbSNP:191054350
2698 2698 a, g dbSNP:759899702
2705 2705 c, t dbSNP:572380293
2767 2767 c, g dbSNP:774641444
2770 2770 a, g dbSNP:560513740
2780 2780 c, t dbSNP:542165602
2784 2784 c, t dbSNP:575039299
2808 2808 c, t dbSNP:56204952
2840 2840 c, t dbSNP:773456371
2873 2873 a, c dbSNP:556431838
2922 2922 a, t dbSNP:186308421
2977 2977 c, t dbSNP:113189011
3012 3012 c, t dbSNP:549991026
3017 3017 c, t dbSNP:138799416
3045 3045 -, agaat dbSNP:374853987
3065 3065 c, t dbSNP:748215074
3074 3074 c, t dbSNP:10625
3087 3087 a, g dbSNP:558265142
3103 3103 a, g dbSNP:1065192
3129 3129 c, t dbSNP:115696450
3130 3130 a, g dbSNP:3024829
3139 3139 a, t dbSNP:745784912
3153 3153 g, t dbSNP:554501590
3159 3159 a, c dbSNP:536163759
3183 3183 c, t dbSNP:531786266
3194 3194 a, g dbSNP:113824447
3200 3200 a, c dbSNP:532373831
3223 3223 c, t dbSNP:371563274
3241 3241 c, t dbSNP:753594106
3326 3326 a, t dbSNP:564463967
3381 3381 a, g dbSNP:201660047
3424 3424 c, t dbSNP:200871431
3425 3425 a, c dbSNP:546289871
3463 3463 a, g dbSNP:3024830
3518 3518 c, t dbSNP:552403996
3522 3522 c, t dbSNP:752187313
3621 3621 a, g dbSNP:527741702
3630 3630 a, t dbSNP:56192916

Target ORF information:

RefSeq Version NM_000542
Organism Homo sapiens (human)
Definition Homo sapiens surfactant protein B (SFTPB), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000542

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACCAAG CAGGGTACCC AGGCTGCAGA GGTGCCATGG CTGAGTCACA CCTGCTGCAG 
TGGCTGCTGC TGCTGCTGCC CACGCTCTGT GGCCCAGGCA CTGCTGCCTG GACCACCTCA
TCCTTGGCCT GTGCCCAGGG CCCTGAGTTC TGGTGCCAAA GCCTGGAGCA AGCATTGCAG
TGCAGAGCCC TAGGGCATTG CCTACAGGAA GTCTGGGGAC ATGTGGGAGC CGATGACCTA
TGCCAAGAGT GTGAGGACAT CGTCCACATC CTTAACAAGA TGGCCAAGGA GGCCATTTTC
CAGGACACGA TGAGGAAGTT CCTGGAGCAG GAGTGCAACG TCCTCCCCTT GAAGCTGCTC
ATGCCCCAGT GCAACCAAGT GCTTGACGAC TACTTCCCCC TGGTCATCGA CTACTTCCAG
AACCAGACTG ACTCAAACGG CATCTGTATG CACCTGGGCC TGTGCAAATC CCGGCAGCCA
GAGCCAGAGC AGGAGCCAGG GATGTCAGAC CCCCTGCCCA AACCTCTGCG GGACCCTCTG
CCAGACCCTC TGCTGGACAA GCTCGTCCTC CCTGTGCTGC CCGGGGCCCT CCAGGCGAGG
CCTGGGCCTC ACACACAGGA TCTCTCCGAG CAGCAATTCC CCATTCCTCT CCCCTATTGC
TGGCTCTGCA GGGCTCTGAT CAAGCGGATC CAAGCCATGA TTCCCAAGGG TGCGCTAGCT
GTGGCAGTGG CCCAGGTGTG CCGCGTGGTA CCTCTGGTGG CGGGCGGCAT CTGCCAGTGC
CTGGCTGAGC GCTACTCCGT CATCCTGCTC GACACGCTGC TGGGCCGCAT GCTGCCCCAG
CTGGTCTGCC GCCTCGTCCT CCGGTGCTCC ATGGATGACA GCGCTGGCCC AAGGTCGCCG
ACAGGAGAAT GGCTGCCGCG AGACTCTGAG TGCCACCTCT GCATGTCCGT GACCACCCAG
GCCGGGAACA GCAGCGAGCA GGCCATACCA CAGGCAATGC TCCAGGCCTG TGTTGGCTCC
TGGCTGGACA GGGAAAAGTG CAAGCAATTT GTGGAGCAGC ACACGCCCCA GCTGCTGACC
CTGGTGCCCA GGGGCTGGGA TGCCCACACC ACCTGCCAGG CCCTCGGGGT GTGTGGGACC
ATGTCCAGCC CTCTCCAGTG TATCCACAGC CCCGACCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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