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SFTPB cDNA ORF clone, Homo sapiens (human)

Gene Symbol SFTPB
Entrez Gene ID 6439
Full Name surfactant protein B
Synonyms PSP-B, SFTB3, SFTP3, SMDP1, SP-B
General protein information
Preferred Names
pulmonary surfactant-associated protein B
Names
pulmonary surfactant-associated protein B
6 kDa protein
18 kDa pulmonary-surfactant protein
pulmonary surfactant-associated proteolipid SPL(Phe)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2p12-p11.2

Summary This gene encodes the pulmonary-associated surfactant protein B (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period. Alternatively spliced transcript variants encoding the same protein have been identified.[provided by RefSeq, Feb 2010]. lac of sum
Disorder MIM:

178640

Disorder Html: Surfactant metabolism dysfunction, pulmonary, 1, 265120 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_005264487 XP_005264544 pulmonary surfactant-associated protein B isoform X1
XM_005264488 XP_005264545 pulmonary surfactant-associated protein B isoform X2
XM_005264490 XP_005264547 pulmonary surfactant-associated protein B isoform X3
NM_198843 NP_942140 pulmonary surfactant-associated protein B precursor
NM_000542 NP_000533 pulmonary surfactant-associated protein B precursor


Homo sapiens (human) SFTPB NP_000533.3
Pan troglodytes (chimpanzee) SFTPB XP_001168379.2
Macaca mulatta (Rhesus monkey) SFTPB XP_001089841.1
Canis lupus familiaris (dog) SFTPB XP_005630540.1
Bos taurus (cattle) SFTPB NP_001068779.1
Mus musculus (house mouse) Sftpb NP_001269000.1
Rattus norvegicus (Norway rat) Sftpb NP_620197.1
Xenopus (Silurana) tropicalis (western clawed frog) sftpb XP_002932589.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SFTPB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SFTPB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu09313 XM_005264487 PREDICTED: Homo sapiens surfactant protein B (SFTPB), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu60453 XM_005264488 PREDICTED: Homo sapiens surfactant protein B (SFTPB), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu60454 XM_005264490 PREDICTED: Homo sapiens surfactant protein B (SFTPB), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu09313 NM_198843 Homo sapiens surfactant protein B (SFTPB), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu09313 NM_000542 Homo sapiens surfactant protein B (SFTPB), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu09313
Clone ID Related Accession (Same CDS sequence) NM_000542 , NM_198843 , XM_005264487
Accession Version XM_005264487.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pulmonary surfactant-associated protein B isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022184.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530367949. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACCAAG CAGGGTACCC AGGCTGCAGA GGTGCCATGG CTGAGTCACA CCTGCTGCAG 
TGGCTGCTGC TGCTGCTGCC CACGCTCTGT GGCCCAGGCA CTGCTGCCTG GACCACCTCA
TCCTTGGCCT GTGCCCAGGG CCCTGAGTTC TGGTGCCAAA GCCTGGAGCA AGCATTGCAG
TGCAGAGCCC TAGGGCATTG CCTACAGGAA GTCTGGGGAC ATGTGGGAGC CGATGACCTA
TGCCAAGAGT GTGAGGACAT CGTCCACATC CTTAACAAGA TGGCCAAGGA GGCCATTTTC
CAGGACACGA TGAGGAAGTT CCTGGAGCAG GAGTGCAACG TCCTCCCCTT GAAGCTGCTC
ATGCCCCAGT GCAACCAAGT GCTTGACGAC TACTTCCCCC TGGTCATCGA CTACTTCCAG
AACCAGACTG ACTCAAACGG CATCTGTATG CACCTGGGCC TGTGCAAATC CCGGCAGCCA
GAGCCAGAGC AGGAGCCAGG GATGTCAGAC CCCCTGCCCA AACCTCTGCG GGACCCTCTG
CCAGACCCTC TGCTGGACAA GCTCGTCCTC CCTGTGCTGC CCGGGGCCCT CCAGGCGAGG
CCTGGGCCTC ACACACAGGA TCTCTCCGAG CAGCAATTCC CCATTCCTCT CCCCTATTGC
TGGCTCTGCA GGGCTCTGAT CAAGCGGATC CAAGCCATGA TTCCCAAGGG TGCGCTAGCT
GTGGCAGTGG CCCAGGTGTG CCGCGTGGTA CCTCTGGTGG CGGGCGGCAT CTGCCAGTGC
CTGGCTGAGC GCTACTCCGT CATCCTGCTC GACACGCTGC TGGGCCGCAT GCTGCCCCAG
CTGGTCTGCC GCCTCGTCCT CCGGTGCTCC ATGGATGACA GCGCTGGCCC AAGGTCGCCG
ACAGGAGAAT GGCTGCCGCG AGACTCTGAG TGCCACCTCT GCATGTCCGT GACCACCCAG
GCCGGGAACA GCAGCGAGCA GGCCATACCA CAGGCAATGC TCCAGGCCTG TGTTGGCTCC
TGGCTGGACA GGGAAAAGTG CAAGCAATTT GTGGAGCAGC ACACGCCCCA GCTGCTGACC
CTGGTGCCCA GGGGCTGGGA TGCCCACACC ACCTGCCAGG CCCTCGGGGT GTGTGGGACC
ATGTCCAGCC CTCTCCAGTG TATCCACAGC CCCGACCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005264544.1
CDS16..1197
Misc Feature(1)133..234(+)
Misc Feature(2)247..363(+)
Misc Feature(3)250..480(+)
Misc Feature(4)379..474(+)
Misc Feature(5)667..882(+)
Misc Feature(6)940..1149(+)
Misc Feature(7)1045..1143(+)
Translation

Target ORF information:

RefSeq Version XM_005264487
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens surfactant protein B (SFTPB), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005264487

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACCAAG CAGGGTACCC AGGCTGCAGA GGTGCCATGG CTGAGTCACA CCTGCTGCAG 
TGGCTGCTGC TGCTGCTGCC CACGCTCTGT GGCCCAGGCA CTGCTGCCTG GACCACCTCA
TCCTTGGCCT GTGCCCAGGG CCCTGAGTTC TGGTGCCAAA GCCTGGAGCA AGCATTGCAG
TGCAGAGCCC TAGGGCATTG CCTACAGGAA GTCTGGGGAC ATGTGGGAGC CGATGACCTA
TGCCAAGAGT GTGAGGACAT CGTCCACATC CTTAACAAGA TGGCCAAGGA GGCCATTTTC
CAGGACACGA TGAGGAAGTT CCTGGAGCAG GAGTGCAACG TCCTCCCCTT GAAGCTGCTC
ATGCCCCAGT GCAACCAAGT GCTTGACGAC TACTTCCCCC TGGTCATCGA CTACTTCCAG
AACCAGACTG ACTCAAACGG CATCTGTATG CACCTGGGCC TGTGCAAATC CCGGCAGCCA
GAGCCAGAGC AGGAGCCAGG GATGTCAGAC CCCCTGCCCA AACCTCTGCG GGACCCTCTG
CCAGACCCTC TGCTGGACAA GCTCGTCCTC CCTGTGCTGC CCGGGGCCCT CCAGGCGAGG
CCTGGGCCTC ACACACAGGA TCTCTCCGAG CAGCAATTCC CCATTCCTCT CCCCTATTGC
TGGCTCTGCA GGGCTCTGAT CAAGCGGATC CAAGCCATGA TTCCCAAGGG TGCGCTAGCT
GTGGCAGTGG CCCAGGTGTG CCGCGTGGTA CCTCTGGTGG CGGGCGGCAT CTGCCAGTGC
CTGGCTGAGC GCTACTCCGT CATCCTGCTC GACACGCTGC TGGGCCGCAT GCTGCCCCAG
CTGGTCTGCC GCCTCGTCCT CCGGTGCTCC ATGGATGACA GCGCTGGCCC AAGGTCGCCG
ACAGGAGAAT GGCTGCCGCG AGACTCTGAG TGCCACCTCT GCATGTCCGT GACCACCCAG
GCCGGGAACA GCAGCGAGCA GGCCATACCA CAGGCAATGC TCCAGGCCTG TGTTGGCTCC
TGGCTGGACA GGGAAAAGTG CAAGCAATTT GTGGAGCAGC ACACGCCCCA GCTGCTGACC
CTGGTGCCCA GGGGCTGGGA TGCCCACACC ACCTGCCAGG CCCTCGGGGT GTGTGGGACC
ATGTCCAGCC CTCTCCAGTG TATCCACAGC CCCGACCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60453
Clone ID Related Accession (Same CDS sequence) XM_005264488
Accession Version XM_005264488.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1134bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pulmonary surfactant-associated protein B isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022184.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530367951. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGAGT CACACCTGCT GCAGTGGCTG CTGCTGCTGC TGCCCACGCT CTGTGGCCCA 
GGCACTGCTG CCTGGACCAC CTCATCCTTG GCCTGTGCCC AGGGCCCTGA GTTCTGGTGC
CAAAGCCTGG AGCAAGCATT GCAGTGCAGA GCCCTAGGGC ATTGCCTACA GGAAGTCTGG
GGACATGTGG GAGCCGATGA CCTATGCCAA GAGTGTGAGG ACATCGTCCA CATCCTTAAC
AAGATGGCCA AGGAGGCCAT TTTCCAGGAC ACGATGAGGA AGTTCCTGGA GCAGGAGTGC
AACGTCCTCC CCTTGAAGCT GCTCATGCCC CAGTGCAACC AAGTGCTTGA CGACTACTTC
CCCCTGGTCA TCGACTACTT CCAGAACCAG ACTGACTCAA ACGGCATCTG TATGCACCTG
GGCCTGTGCA AATCCCGGCA GCCAGAGCCA GAGCAGGAGC CAGGGATGTC AGACCCCCTG
CCCAAACCTC TGCGGGACCC TCTGCCAGAC CCTCTGCTGG ACAAGCTCGT CCTCCCTGTG
CTGCCCGGGG CCCTCCAGGC GAGGCCTGGG CCTCACACAC AGGATCTCTC CGAGCAGCAA
TTCCCCATTC CTCTCCCCTA TTGCTGGCTC TGCAGGGCTC TGATCAAGCG GATCCAAGCC
ATGATTCCCA AGGGTGCGCT AGCTGTGGCA GTGGCCCAGG TGTGCCGCGT GGTACCTCTG
GTGGCGGGCG GCATCTGCCA GTGCCTGGCT GAGCGCTACT CCGTCATCCT GCTCGACACG
CTGCTGGGCC GCATGCTGCC CCAGCTGGTC TGCCGCCTCG TCCTCCGGTG CTCCATGGAT
GACAGCGCTG GCCCAAGAGA ATGGCTGCCG CGAGACTCTG AGTGCCACCT CTGCATGTCC
GTGACCACCC AGGCCGGGAA CAGCAGCGAG CAGGCCATAC CACAGGCAAT GCTCCAGGCC
TGTGTTGGCT CCTGGCTGGA CAGGGAAAAG TGCAAGCAAT TTGTGGAGCA GCACACGCCC
CAGCTGCTGA CCCTGGTGCC CAGGGGCTGG GATGCCCACA CCACCTGCCA GGCCCTCGGG
GTGTGTGGGA CCATGTCCAG CCCTCTCCAG TGTATCCACA GCCCCGACCT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005264545.2
CDS17..1150
Misc Feature(1)98..199(+)
Misc Feature(2)212..328(+)
Misc Feature(3)215..445(+)
Misc Feature(4)344..439(+)
Misc Feature(5)632..847(+)
Misc Feature(6)893..1102(+)
Misc Feature(7)998..1096(+)
Translation

Target ORF information:

RefSeq Version XM_005264488
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens surfactant protein B (SFTPB), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005264488

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGAGT CACACCTGCT GCAGTGGCTG CTGCTGCTGC TGCCCACGCT CTGTGGCCCA 
GGCACTGCTG CCTGGACCAC CTCATCCTTG GCCTGTGCCC AGGGCCCTGA GTTCTGGTGC
CAAAGCCTGG AGCAAGCATT GCAGTGCAGA GCCCTAGGGC ATTGCCTACA GGAAGTCTGG
GGACATGTGG GAGCCGATGA CCTATGCCAA GAGTGTGAGG ACATCGTCCA CATCCTTAAC
AAGATGGCCA AGGAGGCCAT TTTCCAGGAC ACGATGAGGA AGTTCCTGGA GCAGGAGTGC
AACGTCCTCC CCTTGAAGCT GCTCATGCCC CAGTGCAACC AAGTGCTTGA CGACTACTTC
CCCCTGGTCA TCGACTACTT CCAGAACCAG ACTGACTCAA ACGGCATCTG TATGCACCTG
GGCCTGTGCA AATCCCGGCA GCCAGAGCCA GAGCAGGAGC CAGGGATGTC AGACCCCCTG
CCCAAACCTC TGCGGGACCC TCTGCCAGAC CCTCTGCTGG ACAAGCTCGT CCTCCCTGTG
CTGCCCGGGG CCCTCCAGGC GAGGCCTGGG CCTCACACAC AGGATCTCTC CGAGCAGCAA
TTCCCCATTC CTCTCCCCTA TTGCTGGCTC TGCAGGGCTC TGATCAAGCG GATCCAAGCC
ATGATTCCCA AGGGTGCGCT AGCTGTGGCA GTGGCCCAGG TGTGCCGCGT GGTACCTCTG
GTGGCGGGCG GCATCTGCCA GTGCCTGGCT GAGCGCTACT CCGTCATCCT GCTCGACACG
CTGCTGGGCC GCATGCTGCC CCAGCTGGTC TGCCGCCTCG TCCTCCGGTG CTCCATGGAT
GACAGCGCTG GCCCAAGAGA ATGGCTGCCG CGAGACTCTG AGTGCCACCT CTGCATGTCC
GTGACCACCC AGGCCGGGAA CAGCAGCGAG CAGGCCATAC CACAGGCAAT GCTCCAGGCC
TGTGTTGGCT CCTGGCTGGA CAGGGAAAAG TGCAAGCAAT TTGTGGAGCA GCACACGCCC
CAGCTGCTGA CCCTGGTGCC CAGGGGCTGG GATGCCCACA CCACCTGCCA GGCCCTCGGG
GTGTGTGGGA CCATGTCCAG CCCTCTCCAG TGTATCCACA GCCCCGACCT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60454
Clone ID Related Accession (Same CDS sequence) XM_005264490
Accession Version XM_005264490.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pulmonary surfactant-associated protein B isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022184.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578803094. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGAGT CACACCTGCT GCAGTGGCTG CTGCTGCTGC TGCCCACGCT CTGTGGCCCA 
GGCACTGCTG CCTGGACCAC CTCATCCTTG GCCTGTGCCC AGGGCCCTGA GTTCTGGTGC
CAAAGCCTGG AGCAAGCATT GCAGTGCAGA GCCCTAGGGC ATTGCCTACA GGAAGTCTGG
GGACATGTGG GAGCCGATGA CCTATGCCAA GAGTGTGAGG ACATCGTCCA CATCCTTAAC
AAGATGGCCA AGGAGGCCAT TTTCCAGGAC ACGATGAGGA AGTTCCTGGA GCAGGAGTGC
AACGTCCTCC CCTTGAAGCT GCTCATGCCC CAGTGCAACC AAGTGCTTGA CGACTACTTC
CCCCTGGTCA TCGACTACTT CCAGAACCAG ACTGACTCAA ACGGCATCTG TATGCACCTG
GGCCTGTGCA AATCCCGGCA GCCAGAGCCA GAGCAGGAGC CAGGGATGTC AGACCCCCTG
CCCAAACCTC TGCGGGACCC TCTGCCAGAC CCTCTGCTGG ACAAGCTCGT CCTCCCTGTG
CTGCCCGGGG CCCTCCAGGC GAGGCCTGGG CCTCACACAC AGGATCTCTC CGAGCAGCAA
TTCCCCATTC CTCTCCCCTA TTGCTGGCTC TGCAGGGCTC TGATCAAGCG GATCCAAGCC
ATGATTCCCA AGGGTGCGCT AGCTGTGGCA GTGGCCCAGG TGTGCCGCGT GGTACCTCTG
GTGGCGGGCG GCATCTGCCA GTGCCTGGCT GAGCGCTACT CCGTCATCCT GCTCGACACG
CTGCTGGGCC GCATGCTGCC CCAGCTGGTC TGCCGCCTCG TCCTCCGGTG CTCCATGGAT
GACAGCGCTG GCCCAAGGTC GCCGACAGGA GAATGGCTGC CGCGAGACTC TGAGTGCCAC
CTCTGCATGT CCGTGACCAC CCAGGCCGGG AACAGCAGCG AGCAGGCCAT ACCACAGGCA
ATGCTCCAGG CCTGTGTTGG CTCCTGGCTG GACAGGGAAA AGCTGGCTCC AGTTTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005264547.2
CDS17..1036
Misc Feature(1)98..199(+)
Misc Feature(2)212..328(+)
Misc Feature(3)215..445(+)
Misc Feature(4)344..439(+)
Misc Feature(5)632..847(+)
Translation

Target ORF information:

RefSeq Version XM_005264490
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens surfactant protein B (SFTPB), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005264490

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGAGT CACACCTGCT GCAGTGGCTG CTGCTGCTGC TGCCCACGCT CTGTGGCCCA 
GGCACTGCTG CCTGGACCAC CTCATCCTTG GCCTGTGCCC AGGGCCCTGA GTTCTGGTGC
CAAAGCCTGG AGCAAGCATT GCAGTGCAGA GCCCTAGGGC ATTGCCTACA GGAAGTCTGG
GGACATGTGG GAGCCGATGA CCTATGCCAA GAGTGTGAGG ACATCGTCCA CATCCTTAAC
AAGATGGCCA AGGAGGCCAT TTTCCAGGAC ACGATGAGGA AGTTCCTGGA GCAGGAGTGC
AACGTCCTCC CCTTGAAGCT GCTCATGCCC CAGTGCAACC AAGTGCTTGA CGACTACTTC
CCCCTGGTCA TCGACTACTT CCAGAACCAG ACTGACTCAA ACGGCATCTG TATGCACCTG
GGCCTGTGCA AATCCCGGCA GCCAGAGCCA GAGCAGGAGC CAGGGATGTC AGACCCCCTG
CCCAAACCTC TGCGGGACCC TCTGCCAGAC CCTCTGCTGG ACAAGCTCGT CCTCCCTGTG
CTGCCCGGGG CCCTCCAGGC GAGGCCTGGG CCTCACACAC AGGATCTCTC CGAGCAGCAA
TTCCCCATTC CTCTCCCCTA TTGCTGGCTC TGCAGGGCTC TGATCAAGCG GATCCAAGCC
ATGATTCCCA AGGGTGCGCT AGCTGTGGCA GTGGCCCAGG TGTGCCGCGT GGTACCTCTG
GTGGCGGGCG GCATCTGCCA GTGCCTGGCT GAGCGCTACT CCGTCATCCT GCTCGACACG
CTGCTGGGCC GCATGCTGCC CCAGCTGGTC TGCCGCCTCG TCCTCCGGTG CTCCATGGAT
GACAGCGCTG GCCCAAGGTC GCCGACAGGA GAATGGCTGC CGCGAGACTC TGAGTGCCAC
CTCTGCATGT CCGTGACCAC CCAGGCCGGG AACAGCAGCG AGCAGGCCAT ACCACAGGCA
ATGCTCCAGG CCTGTGTTGG CTCCTGGCTG GACAGGGAAA AGCTGGCTCC AGTTTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09313
Clone ID Related Accession (Same CDS sequence) NM_000542 , NM_198843 , XM_005264487
Accession Version NM_198843.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pulmonary surfactant-associated protein B precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL548140.3, BC032785.1, AB439563.1, CA437348.1 and AW204600.1. This sequence is a reference standard in the RefSeqGene project. On Feb 13, 2010 this sequence version replaced gi:38504666. Transcript Variant: This variant (2) lacks an internal segment in the 3' UTR, as compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2147920, SAMEA2155770 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACCAAG CAGGGTACCC AGGCTGCAGA GGTGCCATGG CTGAGTCACA CCTGCTGCAG 
TGGCTGCTGC TGCTGCTGCC CACGCTCTGT GGCCCAGGCA CTGCTGCCTG GACCACCTCA
TCCTTGGCCT GTGCCCAGGG CCCTGAGTTC TGGTGCCAAA GCCTGGAGCA AGCATTGCAG
TGCAGAGCCC TAGGGCATTG CCTACAGGAA GTCTGGGGAC ATGTGGGAGC CGATGACCTA
TGCCAAGAGT GTGAGGACAT CGTCCACATC CTTAACAAGA TGGCCAAGGA GGCCATTTTC
CAGGACACGA TGAGGAAGTT CCTGGAGCAG GAGTGCAACG TCCTCCCCTT GAAGCTGCTC
ATGCCCCAGT GCAACCAAGT GCTTGACGAC TACTTCCCCC TGGTCATCGA CTACTTCCAG
AACCAGACTG ACTCAAACGG CATCTGTATG CACCTGGGCC TGTGCAAATC CCGGCAGCCA
GAGCCAGAGC AGGAGCCAGG GATGTCAGAC CCCCTGCCCA AACCTCTGCG GGACCCTCTG
CCAGACCCTC TGCTGGACAA GCTCGTCCTC CCTGTGCTGC CCGGGGCCCT CCAGGCGAGG
CCTGGGCCTC ACACACAGGA TCTCTCCGAG CAGCAATTCC CCATTCCTCT CCCCTATTGC
TGGCTCTGCA GGGCTCTGAT CAAGCGGATC CAAGCCATGA TTCCCAAGGG TGCGCTAGCT
GTGGCAGTGG CCCAGGTGTG CCGCGTGGTA CCTCTGGTGG CGGGCGGCAT CTGCCAGTGC
CTGGCTGAGC GCTACTCCGT CATCCTGCTC GACACGCTGC TGGGCCGCAT GCTGCCCCAG
CTGGTCTGCC GCCTCGTCCT CCGGTGCTCC ATGGATGACA GCGCTGGCCC AAGGTCGCCG
ACAGGAGAAT GGCTGCCGCG AGACTCTGAG TGCCACCTCT GCATGTCCGT GACCACCCAG
GCCGGGAACA GCAGCGAGCA GGCCATACCA CAGGCAATGC TCCAGGCCTG TGTTGGCTCC
TGGCTGGACA GGGAAAAGTG CAAGCAATTT GTGGAGCAGC ACACGCCCCA GCTGCTGACC
CTGGTGCCCA GGGGCTGGGA TGCCCACACC ACCTGCCAGG CCCTCGGGGT GTGTGGGACC
ATGTCCAGCC CTCTCCAGTG TATCCACAGC CCCGACCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_942140.2
CDS101..1282
Misc Feature(1)92..94(+)
Misc Feature(2)218..319(+)
Misc Feature(3)332..448(+)
Misc Feature(4)335..565(+)
Misc Feature(5)464..559(+)
Misc Feature(6)752..967(+)
Misc Feature(7)1025..1234(+)
Misc Feature(8)1130..1228(+)
Exon (1)1..68
Gene:SFTPB
Gene Synonym:
Exon (2)69..203
Gene:SFTPB
Gene Synonym:
Exon (3)204..331
Gene:SFTPB
Gene Synonym:
Exon (4)332..403
Gene:SFTPB
Gene Synonym:
Exon (5)404..529
Gene:SFTPB
Gene Synonym:
Exon (6)530..718
Gene:SFTPB
Gene Synonym:
Exon (7)719..808
Gene:SFTPB
Gene Synonym:
Exon (8)809..992
Gene:SFTPB
Gene Synonym:
Exon (9)993..1138
Gene:SFTPB
Gene Synonym:
Exon (10)1139..1219
Gene:SFTPB
Gene Synonym:
Exon (11)1220..1301
Gene:SFTPB
Gene Synonym:
Exon (12)1302..2840
Gene:SFTPB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_198843
Organism Homo sapiens (human)
Definition Homo sapiens surfactant protein B (SFTPB), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_198843

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACCAAG CAGGGTACCC AGGCTGCAGA GGTGCCATGG CTGAGTCACA CCTGCTGCAG 
TGGCTGCTGC TGCTGCTGCC CACGCTCTGT GGCCCAGGCA CTGCTGCCTG GACCACCTCA
TCCTTGGCCT GTGCCCAGGG CCCTGAGTTC TGGTGCCAAA GCCTGGAGCA AGCATTGCAG
TGCAGAGCCC TAGGGCATTG CCTACAGGAA GTCTGGGGAC ATGTGGGAGC CGATGACCTA
TGCCAAGAGT GTGAGGACAT CGTCCACATC CTTAACAAGA TGGCCAAGGA GGCCATTTTC
CAGGACACGA TGAGGAAGTT CCTGGAGCAG GAGTGCAACG TCCTCCCCTT GAAGCTGCTC
ATGCCCCAGT GCAACCAAGT GCTTGACGAC TACTTCCCCC TGGTCATCGA CTACTTCCAG
AACCAGACTG ACTCAAACGG CATCTGTATG CACCTGGGCC TGTGCAAATC CCGGCAGCCA
GAGCCAGAGC AGGAGCCAGG GATGTCAGAC CCCCTGCCCA AACCTCTGCG GGACCCTCTG
CCAGACCCTC TGCTGGACAA GCTCGTCCTC CCTGTGCTGC CCGGGGCCCT CCAGGCGAGG
CCTGGGCCTC ACACACAGGA TCTCTCCGAG CAGCAATTCC CCATTCCTCT CCCCTATTGC
TGGCTCTGCA GGGCTCTGAT CAAGCGGATC CAAGCCATGA TTCCCAAGGG TGCGCTAGCT
GTGGCAGTGG CCCAGGTGTG CCGCGTGGTA CCTCTGGTGG CGGGCGGCAT CTGCCAGTGC
CTGGCTGAGC GCTACTCCGT CATCCTGCTC GACACGCTGC TGGGCCGCAT GCTGCCCCAG
CTGGTCTGCC GCCTCGTCCT CCGGTGCTCC ATGGATGACA GCGCTGGCCC AAGGTCGCCG
ACAGGAGAAT GGCTGCCGCG AGACTCTGAG TGCCACCTCT GCATGTCCGT GACCACCCAG
GCCGGGAACA GCAGCGAGCA GGCCATACCA CAGGCAATGC TCCAGGCCTG TGTTGGCTCC
TGGCTGGACA GGGAAAAGTG CAAGCAATTT GTGGAGCAGC ACACGCCCCA GCTGCTGACC
CTGGTGCCCA GGGGCTGGGA TGCCCACACC ACCTGCCAGG CCCTCGGGGT GTGTGGGACC
ATGTCCAGCC CTCTCCAGTG TATCCACAGC CCCGACCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09313
Clone ID Related Accession (Same CDS sequence) NM_000542 , NM_198843 , XM_005264487
Accession Version NM_000542.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pulmonary surfactant-associated protein B precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL548140.3, BC032785.1, BG482852.1, AB439563.1, CA437348.1 and AW204600.1. On Feb 13, 2010 this sequence version replaced gi:33943098. Transcript Variant: This variant (1) is the longer transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2147920, SAMEA2155770 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACCAAG CAGGGTACCC AGGCTGCAGA GGTGCCATGG CTGAGTCACA CCTGCTGCAG 
TGGCTGCTGC TGCTGCTGCC CACGCTCTGT GGCCCAGGCA CTGCTGCCTG GACCACCTCA
TCCTTGGCCT GTGCCCAGGG CCCTGAGTTC TGGTGCCAAA GCCTGGAGCA AGCATTGCAG
TGCAGAGCCC TAGGGCATTG CCTACAGGAA GTCTGGGGAC ATGTGGGAGC CGATGACCTA
TGCCAAGAGT GTGAGGACAT CGTCCACATC CTTAACAAGA TGGCCAAGGA GGCCATTTTC
CAGGACACGA TGAGGAAGTT CCTGGAGCAG GAGTGCAACG TCCTCCCCTT GAAGCTGCTC
ATGCCCCAGT GCAACCAAGT GCTTGACGAC TACTTCCCCC TGGTCATCGA CTACTTCCAG
AACCAGACTG ACTCAAACGG CATCTGTATG CACCTGGGCC TGTGCAAATC CCGGCAGCCA
GAGCCAGAGC AGGAGCCAGG GATGTCAGAC CCCCTGCCCA AACCTCTGCG GGACCCTCTG
CCAGACCCTC TGCTGGACAA GCTCGTCCTC CCTGTGCTGC CCGGGGCCCT CCAGGCGAGG
CCTGGGCCTC ACACACAGGA TCTCTCCGAG CAGCAATTCC CCATTCCTCT CCCCTATTGC
TGGCTCTGCA GGGCTCTGAT CAAGCGGATC CAAGCCATGA TTCCCAAGGG TGCGCTAGCT
GTGGCAGTGG CCCAGGTGTG CCGCGTGGTA CCTCTGGTGG CGGGCGGCAT CTGCCAGTGC
CTGGCTGAGC GCTACTCCGT CATCCTGCTC GACACGCTGC TGGGCCGCAT GCTGCCCCAG
CTGGTCTGCC GCCTCGTCCT CCGGTGCTCC ATGGATGACA GCGCTGGCCC AAGGTCGCCG
ACAGGAGAAT GGCTGCCGCG AGACTCTGAG TGCCACCTCT GCATGTCCGT GACCACCCAG
GCCGGGAACA GCAGCGAGCA GGCCATACCA CAGGCAATGC TCCAGGCCTG TGTTGGCTCC
TGGCTGGACA GGGAAAAGTG CAAGCAATTT GTGGAGCAGC ACACGCCCCA GCTGCTGACC
CTGGTGCCCA GGGGCTGGGA TGCCCACACC ACCTGCCAGG CCCTCGGGGT GTGTGGGACC
ATGTCCAGCC CTCTCCAGTG TATCCACAGC CCCGACCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000533.3
CDS101..1282
Misc Feature(1)92..94(+)
Misc Feature(2)218..319(+)
Misc Feature(3)332..448(+)
Misc Feature(4)335..565(+)
Misc Feature(5)464..559(+)
Misc Feature(6)752..967(+)
Misc Feature(7)1025..1234(+)
Misc Feature(8)1130..1228(+)
Exon (1)1..68
Gene:SFTPB
Gene Synonym:
Exon (2)69..203
Gene:SFTPB
Gene Synonym:
Exon (3)204..331
Gene:SFTPB
Gene Synonym:
Exon (4)332..403
Gene:SFTPB
Gene Synonym:
Exon (5)404..529
Gene:SFTPB
Gene Synonym:
Exon (6)530..718
Gene:SFTPB
Gene Synonym:
Exon (7)719..808
Gene:SFTPB
Gene Synonym:
Exon (8)809..992
Gene:SFTPB
Gene Synonym:
Exon (9)993..1138
Gene:SFTPB
Gene Synonym:
Exon (10)1139..1219
Gene:SFTPB
Gene Synonym:
Exon (11)1220..1301
Gene:SFTPB
Gene Synonym:
Exon (12)1302..3667
Gene:SFTPB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000542
Organism Homo sapiens (human)
Definition Homo sapiens surfactant protein B (SFTPB), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000542

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACCAAG CAGGGTACCC AGGCTGCAGA GGTGCCATGG CTGAGTCACA CCTGCTGCAG 
TGGCTGCTGC TGCTGCTGCC CACGCTCTGT GGCCCAGGCA CTGCTGCCTG GACCACCTCA
TCCTTGGCCT GTGCCCAGGG CCCTGAGTTC TGGTGCCAAA GCCTGGAGCA AGCATTGCAG
TGCAGAGCCC TAGGGCATTG CCTACAGGAA GTCTGGGGAC ATGTGGGAGC CGATGACCTA
TGCCAAGAGT GTGAGGACAT CGTCCACATC CTTAACAAGA TGGCCAAGGA GGCCATTTTC
CAGGACACGA TGAGGAAGTT CCTGGAGCAG GAGTGCAACG TCCTCCCCTT GAAGCTGCTC
ATGCCCCAGT GCAACCAAGT GCTTGACGAC TACTTCCCCC TGGTCATCGA CTACTTCCAG
AACCAGACTG ACTCAAACGG CATCTGTATG CACCTGGGCC TGTGCAAATC CCGGCAGCCA
GAGCCAGAGC AGGAGCCAGG GATGTCAGAC CCCCTGCCCA AACCTCTGCG GGACCCTCTG
CCAGACCCTC TGCTGGACAA GCTCGTCCTC CCTGTGCTGC CCGGGGCCCT CCAGGCGAGG
CCTGGGCCTC ACACACAGGA TCTCTCCGAG CAGCAATTCC CCATTCCTCT CCCCTATTGC
TGGCTCTGCA GGGCTCTGAT CAAGCGGATC CAAGCCATGA TTCCCAAGGG TGCGCTAGCT
GTGGCAGTGG CCCAGGTGTG CCGCGTGGTA CCTCTGGTGG CGGGCGGCAT CTGCCAGTGC
CTGGCTGAGC GCTACTCCGT CATCCTGCTC GACACGCTGC TGGGCCGCAT GCTGCCCCAG
CTGGTCTGCC GCCTCGTCCT CCGGTGCTCC ATGGATGACA GCGCTGGCCC AAGGTCGCCG
ACAGGAGAAT GGCTGCCGCG AGACTCTGAG TGCCACCTCT GCATGTCCGT GACCACCCAG
GCCGGGAACA GCAGCGAGCA GGCCATACCA CAGGCAATGC TCCAGGCCTG TGTTGGCTCC
TGGCTGGACA GGGAAAAGTG CAAGCAATTT GTGGAGCAGC ACACGCCCCA GCTGCTGACC
CTGGTGCCCA GGGGCTGGGA TGCCCACACC ACCTGCCAGG CCCTCGGGGT GTGTGGGACC
ATGTCCAGCC CTCTCCAGTG TATCCACAGC CCCGACCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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