Email to GenScript

SFTPC surfactant protein C [Homo sapiens (human)]

Gene Symbol SFTPC
Entrez Gene ID 6440
Full Name surfactant protein C
Synonyms BRICD6, PSP-C, SFTP2, SMDP2, SP-C
General protein information
Preferred Names
pulmonary surfactant-associated protein C
Names
pulmonary surfactant-associated protein C
SP5
BRICHOS domain containing 6
pulmonary surfactant apoprotein-2 SP-C
pulmonary surfactant-associated proteolipid SPL(Val)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

8

8p21

Summary This gene encodes the pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 2, also called pulmonary alveolar proteinosis due to surfactant protein C deficiency, and are associated with interstitial lung disease in older infants, children, and adults. Alternatively spliced transcript variants encoding different protein isoforms have been identified.[provided by RefSeq, Feb 2010]. lac of sum
Disorder MIM:

178620

Disorder Html: Surfactant metabolism dysfunction, pulmonary, 2, 610913 (3)





The following SFTPC gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SFTPC gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu22858 XM_006716379 PREDICTED: Homo sapiens surfactant protein C (SFTPC), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99
OHu22858 XM_006716380 PREDICTED: Homo sapiens surfactant protein C (SFTPC), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99
OHu60455 XM_011544612 PREDICTED: Homo sapiens surfactant protein C (SFTPC), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99
OHu60455 XM_011544613 PREDICTED: Homo sapiens surfactant protein C (SFTPC), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99
OHu22601 NM_003018 Homo sapiens surfactant protein C (SFTPC), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $99
OHu22858 NM_001172357 Homo sapiens surfactant protein C (SFTPC), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99
OHu22601 NM_001172410 Homo sapiens surfactant protein C (SFTPC), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $99

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu22858
Accession Version XM_006716379.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 576bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pulmonary surfactant-associated protein C isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167187.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578815432. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
ATGGATGTGG GCAGCAAAGA GGTCCTGATG GAGAGCCCGC CGGACTACTC CGCAGCTCCC 
CGGGGCCGAT TTGGCATTCC CTGCTGCCCA GTGCACCTGA AACGCCTTCT TATCGTGGTG
GTGGTGGTGG TCCTCATCGT CGTGGTGATT GTGGGAGCCC TGCTCATGGG TCTCCACATG
AGCCAGAAAC ACACGGAGAT GGTTCTGGAG ATGAGCATTG GGGCGCCGGA AGCCCAGCAA
CGCCTGGCCC TGAGTGAGCA CCTGGTTACC ACTGCCACCT TCTCCATCGG CTCCACTGGC
CTCGTGGTGT ATGACTACCA GCAGCTGCTG ATCGCCTACA AGCCAGCCCC TGGCACCTGC
TGCTACATCA TGAAGATAGC TCCAGAGAGC ATCCCCAGTC TTGAGGCTCT CACTAGAAAA
GTCCACAACT TCCAGGCCAA GCCCGCAGTG CCTACGTCTA AGCTGGGCCA GGCAGAGGGG
CGAGATGCAG GCTCAGCACC CTCCGGAGGG GACCCGGCCT TCCTGGGCAT GGCCGTGAGC
ACCCTGTGTG GCGAGGTGCC GCTCTACTAC ATCTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006716442.1
CDS78..653
Misc Feature(1)78..650(+)
Misc Feature(2)78..356(+)
Misc Feature(3)357..650(+)
Translation
Position Chain Variation Link
1 1 c, g dbSNP:549699699
8 8 c, g dbSNP:750070143
10 10 a, g dbSNP:567738879
11 11 c, t dbSNP:377267823
12 12 a, g dbSNP:367559125
14 14 a, g dbSNP:758318330
21 21 -, aa dbSNP:545529507
21 21 a, t dbSNP:763225526
25 25 a, c, g dbSNP:766625842
26 26 a, c, t dbSNP:373338880
27 27 c, t dbSNP:767971320
34 34 a, c dbSNP:753248473
41 41 -, ggagagga dbSNP:772183067
44 44 g, t dbSNP:756372282
47 47 a, g dbSNP:200665803
52 52 -, gag dbSNP:773257848
60 60 c, t dbSNP:78201008
61 61 a, g dbSNP:758095773
68 68 -, ct dbSNP:760698424
68 68 c, g dbSNP:371755328
75 75 a, c dbSNP:746473012
76 76 a, g dbSNP:768313265
77 77 a, g dbSNP:781209749
87 87 a, g dbSNP:201896861
89 89 a, c dbSNP:564964303
101 101 c, g dbSNP:144603526
103 103 c, t dbSNP:11552815
105 105 a, t dbSNP:773026639
113 113 c, t dbSNP:369413166
116 116 a, c, g dbSNP:532360636
119 119 a, g dbSNP:199905878
120 120 a, g dbSNP:376399274
128 128 c, t dbSNP:752201591
129 129 a, g dbSNP:755609275
131 131 a, g dbSNP:11552816
135 135 c, t dbSNP:777698296
137 137 c, t dbSNP:749232449
139 139 a, g dbSNP:371321234
142 142 a, g dbSNP:778672867
143 143 a, c dbSNP:745700017
144 144 c, t dbSNP:375581850
145 145 a, g, t dbSNP:75902455
150 150 a, g dbSNP:768626980
153 153 a, g dbSNP:368656172
154 154 c, t dbSNP:777139008
157 157 c, t dbSNP:762396958
164 164 c, t dbSNP:770491816
170 170 a, g dbSNP:773682315
171 171 c, t dbSNP:192391655
173 173 c, t dbSNP:767156369
180 180 c, t dbSNP:199854076
191 191 -, gtg dbSNP:762667784
191 191 c, t dbSNP:375940265
192 192 -, gtg dbSNP:752666425
192 192 a, g, t dbSNP:183533911
194 194 -, gtg dbSNP:752783626
207 207 a, g dbSNP:757152791
215 215 c, t dbSNP:548523225
216 216 a, g dbSNP:750172249
218 218 c, g, t dbSNP:764818190
219 219 a, g dbSNP:566914013
221 221 a, g dbSNP:747210099
227 227 g, t dbSNP:768981838
230 230 a, g dbSNP:781417860
234 234 a, g dbSNP:200039720
236 236 c, t dbSNP:376182452
246 246 a, g dbSNP:369239281
253 253 a, g dbSNP:201567623
271 271 c, t dbSNP:547252810
272 272 a, g dbSNP:188074517
273 273 a, g dbSNP:121917836
295 295 a, c, t dbSNP:121917834
302 302 a, g, t dbSNP:369210212
304 304 c, t dbSNP:201454136
305 305 a, c, g dbSNP:75413490
306 306 a, g dbSNP:772841457
318 318 c, t dbSNP:377222742
319 319 a, g dbSNP:766383484
326 326 c, t dbSNP:751292601
333 333 a, g dbSNP:754831645
341 341 a, g dbSNP:370641482
344 344 c, t dbSNP:752977645
353 353 a, c dbSNP:747430307
355 355 c, t dbSNP:777995190
356 356 a, c dbSNP:527723291
361 361 a, c dbSNP:552621627
365 365 c, t dbSNP:79866047
368 368 c, t dbSNP:746180618
371 371 c, t dbSNP:772431756
377 377 c, t dbSNP:377655715
378 378 c, t dbSNP:747785707
380 380 c, t dbSNP:370825102
381 381 a, g dbSNP:772568845
395 395 a, c dbSNP:201933678
405 405 a, c dbSNP:760567018
410 410 c, t dbSNP:764179769
411 411 a, g dbSNP:372529179
416 416 c, t dbSNP:761742367
424 424 a, c dbSNP:121918559
428 428 c, g, t dbSNP:35457216
429 429 c, g dbSNP:758875625
441 441 a, c, t dbSNP:75609798
442 442 a, c dbSNP:77034580
444 444 a, c, g dbSNP:78029713
448 448 c, t dbSNP:755369109
466 466 a, g dbSNP:762396570
476 476 c, t dbSNP:777230179
481 481 -, aggct dbSNP:751323841
484 484 c, t dbSNP:748954723
490 490 a, c dbSNP:4715
491 491 c, t dbSNP:778451328
510 510 c, t dbSNP:745419854
513 513 a, g dbSNP:781120153
520 520 c, t dbSNP:752593536
522 522 c, g dbSNP:202145169
526 526 g, t dbSNP:777510956
528 528 c, t dbSNP:749126105
531 531 a, g dbSNP:771089238
533 533 c, g dbSNP:373783536
539 539 a, g dbSNP:745893171
549 549 a, g dbSNP:371013652
557 557 a, g dbSNP:775696772
559 559 a, g dbSNP:34957318
567 567 a, g dbSNP:769328550
571 571 c, t dbSNP:777114058
579 579 a, t dbSNP:546357906
582 582 a, g dbSNP:766093799
586 586 a, g dbSNP:537383138
592 592 c, t dbSNP:759031390
593 593 a, g dbSNP:149606090
594 594 c, g dbSNP:752289567
595 595 c, t dbSNP:531634308
600 600 c, g dbSNP:201685063
606 606 a, g dbSNP:777707126
607 607 g, t dbSNP:753589128
609 609 a, g dbSNP:757050514
611 611 c, t dbSNP:779136923
616 616 a, g dbSNP:1124
622 622 a, t dbSNP:121917835
624 624 c, t dbSNP:76821562
629 629 c, t dbSNP:529959941
630 630 a, g dbSNP:200839715
632 632 a, c, g dbSNP:747175730
634 634 c, t dbSNP:769238316
640 640 c, t dbSNP:121918560
650 650 c, t dbSNP:3203602
652 652 a, g dbSNP:777312443
653 653 c, g dbSNP:370068089
660 660 g, t dbSNP:566370122
663 663 a, g dbSNP:773687273
665 665 a, c, t dbSNP:200469074
669 669 a, c, g dbSNP:12229
681 681 a, g dbSNP:1804453
689 689 a, g dbSNP:75518353
693 693 g, t dbSNP:543423602
708 708 a, g dbSNP:748654127
764 764 c, g dbSNP:565381858
765 765 c, t dbSNP:562115512
767 767 c, t dbSNP:11552817
776 776 a, g dbSNP:7592
782 782 a, g dbSNP:749681094
792 792 a, g dbSNP:541769233
826 826 c, t dbSNP:1126931
827 827 a, g, t dbSNP:1139547
830 830 c, t dbSNP:552366898
834 834 a, g dbSNP:763580777
835 835 c, g, t dbSNP:8192331
845 845 c, t dbSNP:776190058
851 851 a, c dbSNP:761708440
869 869 c, t dbSNP:8192342
874 874 c, t dbSNP:765269411

Target ORF information:

RefSeq Version XM_006716379
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens surfactant protein C (SFTPC), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006716379

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGATGTGG GCAGCAAAGA GGTCCTGATG GAGAGCCCGC CGGACTACTC CGCAGCTCCC 
CGGGGCCGAT TTGGCATTCC CTGCTGCCCA GTGCACCTGA AACGCCTTCT TATCGTGGTG
GTGGTGGTGG TCCTCATCGT CGTGGTGATT GTGGGAGCCC TGCTCATGGG TCTCCACATG
AGCCAGAAAC ACACGGAGAT GGTTCTGGAG ATGAGCATTG GGGCGCCGGA AGCCCAGCAA
CGCCTGGCCC TGAGTGAGCA CCTGGTTACC ACTGCCACCT TCTCCATCGG CTCCACTGGC
CTCGTGGTGT ATGACTACCA GCAGCTGCTG ATCGCCTACA AGCCAGCCCC TGGCACCTGC
TGCTACATCA TGAAGATAGC TCCAGAGAGC ATCCCCAGTC TTGAGGCTCT CACTAGAAAA
GTCCACAACT TCCAGGCCAA GCCCGCAGTG CCTACGTCTA AGCTGGGCCA GGCAGAGGGG
CGAGATGCAG GCTCAGCACC CTCCGGAGGG GACCCGGCCT TCCTGGGCAT GGCCGTGAGC
ACCCTGTGTG GCGAGGTGCC GCTCTACTAC ATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22858
Accession Version XM_006716380.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 576bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pulmonary surfactant-associated protein C isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167187.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578815434. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
ATGGATGTGG GCAGCAAAGA GGTCCTGATG GAGAGCCCGC CGGACTACTC CGCAGCTCCC 
CGGGGCCGAT TTGGCATTCC CTGCTGCCCA GTGCACCTGA AACGCCTTCT TATCGTGGTG
GTGGTGGTGG TCCTCATCGT CGTGGTGATT GTGGGAGCCC TGCTCATGGG TCTCCACATG
AGCCAGAAAC ACACGGAGAT GGTTCTGGAG ATGAGCATTG GGGCGCCGGA AGCCCAGCAA
CGCCTGGCCC TGAGTGAGCA CCTGGTTACC ACTGCCACCT TCTCCATCGG CTCCACTGGC
CTCGTGGTGT ATGACTACCA GCAGCTGCTG ATCGCCTACA AGCCAGCCCC TGGCACCTGC
TGCTACATCA TGAAGATAGC TCCAGAGAGC ATCCCCAGTC TTGAGGCTCT CACTAGAAAA
GTCCACAACT TCCAGGCCAA GCCCGCAGTG CCTACGTCTA AGCTGGGCCA GGCAGAGGGG
CGAGATGCAG GCTCAGCACC CTCCGGAGGG GACCCGGCCT TCCTGGGCAT GGCCGTGAGC
ACCCTGTGTG GCGAGGTGCC GCTCTACTAC ATCTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006716443.1
CDS79..654
Misc Feature(1)79..651(+)
Misc Feature(2)79..357(+)
Misc Feature(3)358..651(+)
Translation
Position Chain Variation Link
1 1 a, c dbSNP:762050676
2 2 c, g dbSNP:549699699
9 9 c, g dbSNP:750070143
11 11 a, g dbSNP:567738879
12 12 c, t dbSNP:377267823
13 13 a, g dbSNP:367559125
15 15 a, g dbSNP:758318330
22 22 -, aa dbSNP:545529507
22 22 a, t dbSNP:763225526
26 26 a, c, g dbSNP:766625842
27 27 a, c, t dbSNP:373338880
28 28 c, t dbSNP:767971320
35 35 a, c dbSNP:753248473
42 42 -, ggagagga dbSNP:772183067
45 45 g, t dbSNP:756372282
48 48 a, g dbSNP:200665803
53 53 -, gag dbSNP:773257848
61 61 c, t dbSNP:78201008
62 62 a, g dbSNP:758095773
69 69 -, ct dbSNP:760698424
69 69 c, g dbSNP:371755328
76 76 a, c dbSNP:746473012
77 77 a, g dbSNP:768313265
78 78 a, g dbSNP:781209749
88 88 a, g dbSNP:201896861
90 90 a, c dbSNP:564964303
102 102 c, g dbSNP:144603526
104 104 c, t dbSNP:11552815
106 106 a, t dbSNP:773026639
114 114 c, t dbSNP:369413166
117 117 a, c, g dbSNP:532360636
120 120 a, g dbSNP:199905878
121 121 a, g dbSNP:376399274
129 129 c, t dbSNP:752201591
130 130 a, g dbSNP:755609275
132 132 a, g dbSNP:11552816
136 136 c, t dbSNP:777698296
138 138 c, t dbSNP:749232449
140 140 a, g dbSNP:371321234
143 143 a, g dbSNP:778672867
144 144 a, c dbSNP:745700017
145 145 c, t dbSNP:375581850
146 146 a, g, t dbSNP:75902455
151 151 a, g dbSNP:768626980
154 154 a, g dbSNP:368656172
155 155 c, t dbSNP:777139008
158 158 c, t dbSNP:762396958
165 165 c, t dbSNP:770491816
171 171 a, g dbSNP:773682315
172 172 c, t dbSNP:192391655
174 174 c, t dbSNP:767156369
181 181 c, t dbSNP:199854076
192 192 -, gtg dbSNP:762667784
192 192 c, t dbSNP:375940265
193 193 -, gtg dbSNP:752666425
193 193 a, g, t dbSNP:183533911
195 195 -, gtg dbSNP:752783626
208 208 a, g dbSNP:757152791
216 216 c, t dbSNP:548523225
217 217 a, g dbSNP:750172249
219 219 c, g, t dbSNP:764818190
220 220 a, g dbSNP:566914013
222 222 a, g dbSNP:747210099
228 228 g, t dbSNP:768981838
231 231 a, g dbSNP:781417860
235 235 a, g dbSNP:200039720
237 237 c, t dbSNP:376182452
247 247 a, g dbSNP:369239281
254 254 a, g dbSNP:201567623
272 272 c, t dbSNP:547252810
273 273 a, g dbSNP:188074517
274 274 a, g dbSNP:121917836
296 296 a, c, t dbSNP:121917834
303 303 a, g, t dbSNP:369210212
305 305 c, t dbSNP:201454136
306 306 a, c, g dbSNP:75413490
307 307 a, g dbSNP:772841457
319 319 c, t dbSNP:377222742
320 320 a, g dbSNP:766383484
327 327 c, t dbSNP:751292601
334 334 a, g dbSNP:754831645
342 342 a, g dbSNP:370641482
345 345 c, t dbSNP:752977645
354 354 a, c dbSNP:747430307
356 356 c, t dbSNP:777995190
357 357 a, c dbSNP:527723291
362 362 a, c dbSNP:552621627
366 366 c, t dbSNP:79866047
369 369 c, t dbSNP:746180618
372 372 c, t dbSNP:772431756
378 378 c, t dbSNP:377655715
379 379 c, t dbSNP:747785707
381 381 c, t dbSNP:370825102
382 382 a, g dbSNP:772568845
396 396 a, c dbSNP:201933678
406 406 a, c dbSNP:760567018
411 411 c, t dbSNP:764179769
412 412 a, g dbSNP:372529179
417 417 c, t dbSNP:761742367
425 425 a, c dbSNP:121918559
429 429 c, g, t dbSNP:35457216
430 430 c, g dbSNP:758875625
442 442 a, c, t dbSNP:75609798
443 443 a, c dbSNP:77034580
445 445 a, c, g dbSNP:78029713
449 449 c, t dbSNP:755369109
467 467 a, g dbSNP:762396570
477 477 c, t dbSNP:777230179
482 482 -, aggct dbSNP:751323841
485 485 c, t dbSNP:748954723
491 491 a, c dbSNP:4715
492 492 c, t dbSNP:778451328
511 511 c, t dbSNP:745419854
514 514 a, g dbSNP:781120153
521 521 c, t dbSNP:752593536
523 523 c, g dbSNP:202145169
527 527 g, t dbSNP:777510956
529 529 c, t dbSNP:749126105
532 532 a, g dbSNP:771089238
534 534 c, g dbSNP:373783536
540 540 a, g dbSNP:745893171
550 550 a, g dbSNP:371013652
558 558 a, g dbSNP:775696772
560 560 a, g dbSNP:34957318
568 568 a, g dbSNP:769328550
572 572 c, t dbSNP:777114058
580 580 a, t dbSNP:546357906
583 583 a, g dbSNP:766093799
587 587 a, g dbSNP:537383138
593 593 c, t dbSNP:759031390
594 594 a, g dbSNP:149606090
595 595 c, g dbSNP:752289567
596 596 c, t dbSNP:531634308
601 601 c, g dbSNP:201685063
607 607 a, g dbSNP:777707126
608 608 g, t dbSNP:753589128
610 610 a, g dbSNP:757050514
612 612 c, t dbSNP:779136923
617 617 a, g dbSNP:1124
623 623 a, t dbSNP:121917835
625 625 c, t dbSNP:76821562
630 630 c, t dbSNP:529959941
631 631 a, g dbSNP:200839715
633 633 a, c, g dbSNP:747175730
635 635 c, t dbSNP:769238316
641 641 c, t dbSNP:121918560
651 651 c, t dbSNP:3203602
653 653 a, g dbSNP:777312443
654 654 c, g dbSNP:370068089
661 661 g, t dbSNP:566370122
664 664 a, g dbSNP:773687273
674 674 a, g dbSNP:1804453
682 682 a, g dbSNP:75518353
686 686 g, t dbSNP:543423602
701 701 a, g dbSNP:748654127
757 757 c, g dbSNP:565381858
758 758 c, t dbSNP:562115512
760 760 c, t dbSNP:11552817
769 769 a, g dbSNP:7592
775 775 a, g dbSNP:749681094
785 785 a, g dbSNP:541769233
819 819 c, t dbSNP:1126931
820 820 a, g, t dbSNP:1139547
823 823 c, t dbSNP:552366898
827 827 a, g dbSNP:763580777
828 828 c, g, t dbSNP:8192331
838 838 c, t dbSNP:776190058
844 844 a, c dbSNP:761708440
862 862 c, t dbSNP:8192342
867 867 c, t dbSNP:765269411

Target ORF information:

RefSeq Version XM_006716380
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens surfactant protein C (SFTPC), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006716380

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGATGTGG GCAGCAAAGA GGTCCTGATG GAGAGCCCGC CGGACTACTC CGCAGCTCCC 
CGGGGCCGAT TTGGCATTCC CTGCTGCCCA GTGCACCTGA AACGCCTTCT TATCGTGGTG
GTGGTGGTGG TCCTCATCGT CGTGGTGATT GTGGGAGCCC TGCTCATGGG TCTCCACATG
AGCCAGAAAC ACACGGAGAT GGTTCTGGAG ATGAGCATTG GGGCGCCGGA AGCCCAGCAA
CGCCTGGCCC TGAGTGAGCA CCTGGTTACC ACTGCCACCT TCTCCATCGG CTCCACTGGC
CTCGTGGTGT ATGACTACCA GCAGCTGCTG ATCGCCTACA AGCCAGCCCC TGGCACCTGC
TGCTACATCA TGAAGATAGC TCCAGAGAGC ATCCCCAGTC TTGAGGCTCT CACTAGAAAA
GTCCACAACT TCCAGGCCAA GCCCGCAGTG CCTACGTCTA AGCTGGGCCA GGCAGAGGGG
CGAGATGCAG GCTCAGCACC CTCCGGAGGG GACCCGGCCT TCCTGGGCAT GGCCGTGAGC
ACCCTGTGTG GCGAGGTGCC GCTCTACTAC ATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60455
Accession Version XM_011544612.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 435bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pulmonary surfactant-associated protein C isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167187.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
ATGGATGTGG GCAGCAAAGA GGTCCTGATG GAGAGCCCGC CGGTTCTGGA GATGAGCATT 
GGGGCGCCGG AAGCCCAGCA ACGCCTGGCC CTGAGTGAGC ACCTGGTTAC CACTGCCACC
TTCTCCATCG GCTCCACTGG CCTCGTGGTG TATGACTACC AGCAGCTGCT GATCGCCTAC
AAGCCAGCCC CTGGCACCTG CTGCTACATC ATGAAGATAG CTCCAGAGAG CATCCCCAGT
CTTGAGGCTC TCACTAGAAA AGTCCACAAC TTCCAGATGG AATGCTCTCT GCAGGCCAAG
CCCGCAGTGC CTACGTCTAA GCTGGGCCAG GCAGAGGGGC GAGATGCAGG CTCAGCACCC
TCCGGAGGGG ACCCGGCCTT CCTGGGCATG GCCGTGAGCA CCCTGTGTGG CGAGGTGCCG
CTCTACTACA TCTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011542914.1
CDS95..529
Misc Feature(1)95..526(+)
Misc Feature(2)95..214(+)
Misc Feature(3)215..526(+)
Translation
Position Chain Variation Link
3 3 c, g dbSNP:79647630
4 4 c, t dbSNP:77253713
6 6 a, t dbSNP:779710215
12 12 c, g dbSNP:746560865
17 17 a, c dbSNP:762050676
18 18 c, g dbSNP:549699699
25 25 c, g dbSNP:750070143
27 27 a, g dbSNP:567738879
28 28 c, t dbSNP:377267823
29 29 a, g dbSNP:367559125
31 31 a, g dbSNP:758318330
38 38 -, aa dbSNP:545529507
38 38 a, t dbSNP:763225526
42 42 a, c, g dbSNP:766625842
43 43 a, c, t dbSNP:373338880
44 44 c, t dbSNP:767971320
51 51 a, c dbSNP:753248473
58 58 -, ggagagga dbSNP:772183067
61 61 g, t dbSNP:756372282
64 64 a, g dbSNP:200665803
69 69 -, gag dbSNP:773257848
77 77 c, t dbSNP:78201008
78 78 a, g dbSNP:758095773
85 85 -, ct dbSNP:760698424
85 85 c, g dbSNP:371755328
92 92 a, c dbSNP:746473012
93 93 a, g dbSNP:768313265
94 94 a, g dbSNP:781209749
104 104 a, g dbSNP:201896861
106 106 a, c dbSNP:564964303
118 118 c, g dbSNP:144603526
120 120 c, t dbSNP:11552815
122 122 a, t dbSNP:773026639
130 130 c, t dbSNP:369413166
133 133 a, c, g dbSNP:532360636
136 136 a, g dbSNP:199905878
153 153 a, c, t dbSNP:121917834
160 160 a, g, t dbSNP:369210212
162 162 c, t dbSNP:201454136
163 163 a, c, g dbSNP:75413490
164 164 a, g dbSNP:772841457
176 176 c, t dbSNP:377222742
177 177 a, g dbSNP:766383484
184 184 c, t dbSNP:751292601
191 191 a, g dbSNP:754831645
199 199 a, g dbSNP:370641482
202 202 c, t dbSNP:752977645
211 211 a, c dbSNP:747430307
213 213 c, t dbSNP:777995190
214 214 a, c dbSNP:527723291
219 219 a, c dbSNP:552621627
223 223 c, t dbSNP:79866047
226 226 c, t dbSNP:746180618
229 229 c, t dbSNP:772431756
235 235 c, t dbSNP:377655715
236 236 c, t dbSNP:747785707
238 238 c, t dbSNP:370825102
239 239 a, g dbSNP:772568845
253 253 a, c dbSNP:201933678
263 263 a, c dbSNP:760567018
268 268 c, t dbSNP:764179769
269 269 a, g dbSNP:372529179
274 274 c, t dbSNP:761742367
282 282 a, c dbSNP:121918559
286 286 c, g, t dbSNP:35457216
287 287 c, g dbSNP:758875625
299 299 a, c, t dbSNP:75609798
300 300 a, c dbSNP:77034580
302 302 a, c, g dbSNP:78029713
306 306 c, t dbSNP:755369109
324 324 a, g dbSNP:762396570
334 334 c, t dbSNP:777230179
339 339 -, aggct dbSNP:751323841
342 342 c, t dbSNP:748954723
348 348 a, c dbSNP:4715
349 349 c, t dbSNP:778451328
368 368 c, t dbSNP:745419854
374 374 a, g dbSNP:374909143
378 378 a, g dbSNP:766184622
379 379 c, t dbSNP:751034722
381 381 c, t dbSNP:754481467
389 389 a, g dbSNP:781120153
396 396 c, t dbSNP:752593536
398 398 c, g dbSNP:202145169
402 402 g, t dbSNP:777510956
404 404 c, t dbSNP:749126105
407 407 a, g dbSNP:771089238
409 409 c, g dbSNP:373783536
415 415 a, g dbSNP:745893171
425 425 a, g dbSNP:371013652
433 433 a, g dbSNP:775696772
435 435 a, g dbSNP:34957318
443 443 a, g dbSNP:769328550
447 447 c, t dbSNP:777114058
455 455 a, t dbSNP:546357906
458 458 a, g dbSNP:766093799
462 462 a, g dbSNP:537383138
468 468 c, t dbSNP:759031390
469 469 a, g dbSNP:149606090
470 470 c, g dbSNP:752289567
471 471 c, t dbSNP:531634308
476 476 c, g dbSNP:201685063
482 482 a, g dbSNP:777707126
483 483 g, t dbSNP:753589128
485 485 a, g dbSNP:757050514
487 487 c, t dbSNP:779136923
492 492 a, g dbSNP:1124
498 498 a, t dbSNP:121917835
500 500 c, t dbSNP:76821562
505 505 c, t dbSNP:529959941
506 506 a, g dbSNP:200839715
508 508 a, c, g dbSNP:747175730
510 510 c, t dbSNP:769238316
516 516 c, t dbSNP:121918560
526 526 c, t dbSNP:3203602
528 528 a, g dbSNP:777312443
529 529 c, g dbSNP:370068089
536 536 g, t dbSNP:566370122
539 539 a, g dbSNP:773687273
541 541 a, c, t dbSNP:200469074
545 545 a, c, g dbSNP:12229
557 557 a, g dbSNP:1804453
565 565 a, g dbSNP:75518353
569 569 g, t dbSNP:543423602
584 584 a, g dbSNP:748654127
640 640 c, g dbSNP:565381858
641 641 c, t dbSNP:562115512
643 643 c, t dbSNP:11552817
652 652 a, g dbSNP:7592
658 658 a, g dbSNP:749681094
668 668 a, g dbSNP:541769233
702 702 c, t dbSNP:1126931
703 703 a, g, t dbSNP:1139547
706 706 c, t dbSNP:552366898
710 710 a, g dbSNP:763580777
711 711 c, g, t dbSNP:8192331
721 721 c, t dbSNP:776190058
727 727 a, c dbSNP:761708440
745 745 c, t dbSNP:8192342
750 750 c, t dbSNP:765269411

Target ORF information:

RefSeq Version XM_011544612
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens surfactant protein C (SFTPC), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011544612

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGATGTGG GCAGCAAAGA GGTCCTGATG GAGAGCCCGC CGGTTCTGGA GATGAGCATT 
GGGGCGCCGG AAGCCCAGCA ACGCCTGGCC CTGAGTGAGC ACCTGGTTAC CACTGCCACC
TTCTCCATCG GCTCCACTGG CCTCGTGGTG TATGACTACC AGCAGCTGCT GATCGCCTAC
AAGCCAGCCC CTGGCACCTG CTGCTACATC ATGAAGATAG CTCCAGAGAG CATCCCCAGT
CTTGAGGCTC TCACTAGAAA AGTCCACAAC TTCCAGATGG AATGCTCTCT GCAGGCCAAG
CCCGCAGTGC CTACGTCTAA GCTGGGCCAG GCAGAGGGGC GAGATGCAGG CTCAGCACCC
TCCGGAGGGG ACCCGGCCTT CCTGGGCATG GCCGTGAGCA CCCTGTGTGG CGAGGTGCCG
CTCTACTACA TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60455
Accession Version XM_011544613.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 435bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product pulmonary surfactant-associated protein C isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167187.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
ATGGATGTGG GCAGCAAAGA GGTCCTGATG GAGAGCCCGC CGGTTCTGGA GATGAGCATT 
GGGGCGCCGG AAGCCCAGCA ACGCCTGGCC CTGAGTGAGC ACCTGGTTAC CACTGCCACC
TTCTCCATCG GCTCCACTGG CCTCGTGGTG TATGACTACC AGCAGCTGCT GATCGCCTAC
AAGCCAGCCC CTGGCACCTG CTGCTACATC ATGAAGATAG CTCCAGAGAG CATCCCCAGT
CTTGAGGCTC TCACTAGAAA AGTCCACAAC TTCCAGATGG AATGCTCTCT GCAGGCCAAG
CCCGCAGTGC CTACGTCTAA GCTGGGCCAG GCAGAGGGGC GAGATGCAGG CTCAGCACCC
TCCGGAGGGG ACCCGGCCTT CCTGGGCATG GCCGTGAGCA CCCTGTGTGG CGAGGTGCCG
CTCTACTACA TCTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011542915.1
CDS97..531
Misc Feature(1)97..528(+)
Misc Feature(2)97..216(+)
Misc Feature(3)217..528(+)
Translation
Position Chain Variation Link
2 2 g, t dbSNP:778358826
5 5 c, g dbSNP:79647630
6 6 c, t dbSNP:77253713
8 8 a, t dbSNP:779710215
14 14 c, g dbSNP:746560865
19 19 a, c dbSNP:762050676
20 20 c, g dbSNP:549699699
27 27 c, g dbSNP:750070143
29 29 a, g dbSNP:567738879
30 30 c, t dbSNP:377267823
31 31 a, g dbSNP:367559125
33 33 a, g dbSNP:758318330
40 40 -, aa dbSNP:545529507
40 40 a, t dbSNP:763225526
44 44 a, c, g dbSNP:766625842
45 45 a, c, t dbSNP:373338880
46 46 c, t dbSNP:767971320
53 53 a, c dbSNP:753248473
60 60 -, ggagagga dbSNP:772183067
63 63 g, t dbSNP:756372282
66 66 a, g dbSNP:200665803
71 71 -, gag dbSNP:773257848
79 79 c, t dbSNP:78201008
80 80 a, g dbSNP:758095773
87 87 -, ct dbSNP:760698424
87 87 c, g dbSNP:371755328
94 94 a, c dbSNP:746473012
95 95 a, g dbSNP:768313265
96 96 a, g dbSNP:781209749
106 106 a, g dbSNP:201896861
108 108 a, c dbSNP:564964303
120 120 c, g dbSNP:144603526
122 122 c, t dbSNP:11552815
124 124 a, t dbSNP:773026639
132 132 c, t dbSNP:369413166
135 135 a, c, g dbSNP:532360636
138 138 a, g dbSNP:199905878
155 155 a, c, t dbSNP:121917834
162 162 a, g, t dbSNP:369210212
164 164 c, t dbSNP:201454136
165 165 a, c, g dbSNP:75413490
166 166 a, g dbSNP:772841457
178 178 c, t dbSNP:377222742
179 179 a, g dbSNP:766383484
186 186 c, t dbSNP:751292601
193 193 a, g dbSNP:754831645
201 201 a, g dbSNP:370641482
204 204 c, t dbSNP:752977645
213 213 a, c dbSNP:747430307
215 215 c, t dbSNP:777995190
216 216 a, c dbSNP:527723291
221 221 a, c dbSNP:552621627
225 225 c, t dbSNP:79866047
228 228 c, t dbSNP:746180618
231 231 c, t dbSNP:772431756
237 237 c, t dbSNP:377655715
238 238 c, t dbSNP:747785707
240 240 c, t dbSNP:370825102
241 241 a, g dbSNP:772568845
255 255 a, c dbSNP:201933678
265 265 a, c dbSNP:760567018
270 270 c, t dbSNP:764179769
271 271 a, g dbSNP:372529179
276 276 c, t dbSNP:761742367
284 284 a, c dbSNP:121918559
288 288 c, g, t dbSNP:35457216
289 289 c, g dbSNP:758875625
301 301 a, c, t dbSNP:75609798
302 302 a, c dbSNP:77034580
304 304 a, c, g dbSNP:78029713
308 308 c, t dbSNP:755369109
326 326 a, g dbSNP:762396570
336 336 c, t dbSNP:777230179
341 341 -, aggct dbSNP:751323841
344 344 c, t dbSNP:748954723
350 350 a, c dbSNP:4715
351 351 c, t dbSNP:778451328
370 370 c, t dbSNP:745419854
376 376 a, g dbSNP:374909143
380 380 a, g dbSNP:766184622
381 381 c, t dbSNP:751034722
383 383 c, t dbSNP:754481467
391 391 a, g dbSNP:781120153
398 398 c, t dbSNP:752593536
400 400 c, g dbSNP:202145169
404 404 g, t dbSNP:777510956
406 406 c, t dbSNP:749126105
409 409 a, g dbSNP:771089238
411 411 c, g dbSNP:373783536
417 417 a, g dbSNP:745893171
427 427 a, g dbSNP:371013652
435 435 a, g dbSNP:775696772
437 437 a, g dbSNP:34957318
445 445 a, g dbSNP:769328550
449 449 c, t dbSNP:777114058
457 457 a, t dbSNP:546357906
460 460 a, g dbSNP:766093799
464 464 a, g dbSNP:537383138
470 470 c, t dbSNP:759031390
471 471 a, g dbSNP:149606090
472 472 c, g dbSNP:752289567
473 473 c, t dbSNP:531634308
478 478 c, g dbSNP:201685063
484 484 a, g dbSNP:777707126
485 485 g, t dbSNP:753589128
487 487 a, g dbSNP:757050514
489 489 c, t dbSNP:779136923
494 494 a, g dbSNP:1124
500 500 a, t dbSNP:121917835
502 502 c, t dbSNP:76821562
507 507 c, t dbSNP:529959941
508 508 a, g dbSNP:200839715
510 510 a, c, g dbSNP:747175730
512 512 c, t dbSNP:769238316
518 518 c, t dbSNP:121918560
528 528 c, t dbSNP:3203602
530 530 a, g dbSNP:777312443
531 531 c, g dbSNP:370068089
538 538 g, t dbSNP:566370122
541 541 a, g dbSNP:773687273
551 551 a, g dbSNP:1804453
559 559 a, g dbSNP:75518353
563 563 g, t dbSNP:543423602
578 578 a, g dbSNP:748654127
634 634 c, g dbSNP:565381858
635 635 c, t dbSNP:562115512
637 637 c, t dbSNP:11552817
646 646 a, g dbSNP:7592
652 652 a, g dbSNP:749681094
662 662 a, g dbSNP:541769233
696 696 c, t dbSNP:1126931
697 697 a, g, t dbSNP:1139547
700 700 c, t dbSNP:552366898
704 704 a, g dbSNP:763580777
705 705 c, g, t dbSNP:8192331
715 715 c, t dbSNP:776190058
721 721 a, c dbSNP:761708440
739 739 c, t dbSNP:8192342
744 744 c, t dbSNP:765269411

Target ORF information:

RefSeq Version XM_011544613
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens surfactant protein C (SFTPC), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011544613

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGATGTGG GCAGCAAAGA GGTCCTGATG GAGAGCCCGC CGGTTCTGGA GATGAGCATT 
GGGGCGCCGG AAGCCCAGCA ACGCCTGGCC CTGAGTGAGC ACCTGGTTAC CACTGCCACC
TTCTCCATCG GCTCCACTGG CCTCGTGGTG TATGACTACC AGCAGCTGCT GATCGCCTAC
AAGCCAGCCC CTGGCACCTG CTGCTACATC ATGAAGATAG CTCCAGAGAG CATCCCCAGT
CTTGAGGCTC TCACTAGAAA AGTCCACAAC TTCCAGATGG AATGCTCTCT GCAGGCCAAG
CCCGCAGTGC CTACGTCTAA GCTGGGCCAG GCAGAGGGGC GAGATGCAGG CTCAGCACCC
TCCGGAGGGG ACCCGGCCTT CCTGGGCATG GCCGTGAGCA CCCTGTGTGG CGAGGTGCCG
CTCTACTACA TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22601
Accession Version NM_003018.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 594bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pulmonary surfactant-associated protein C isoform 1 proprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J03553.1, BC105594.1, DB537043.1 and J03517.1. This sequence is a reference standard in the RefSeqGene project. On Jun 28, 2007 this sequence version replaced gi:42476334. Summary: This gene encodes the pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 2, also called pulmonary alveolar proteinosis due to surfactant protein C deficiency, and are associated with interstitial lung disease in older infants, children, and adults. Alternatively spliced transcript variants encoding different protein isoforms have been identified.[provided by RefSeq, Feb 2010]. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J03517.1, J03553.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2142363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGATGTGG GCAGCAAAGA GGTCCTGATG GAGAGCCCGC CGGACTACTC CGCAGCTCCC 
CGGGGCCGAT TTGGCATTCC CTGCTGCCCA GTGCACCTGA AACGCCTTCT TATCGTGGTG
GTGGTGGTGG TCCTCATCGT CGTGGTGATT GTGGGAGCCC TGCTCATGGG TCTCCACATG
AGCCAGAAAC ACACGGAGAT GGTTCTGGAG ATGAGCATTG GGGCGCCGGA AGCCCAGCAA
CGCCTGGCCC TGAGTGAGCA CCTGGTTACC ACTGCCACCT TCTCCATCGG CTCCACTGGC
CTCGTGGTGT ATGACTACCA GCAGCTGCTG ATCGCCTACA AGCCAGCCCC TGGCACCTGC
TGCTACATCA TGAAGATAGC TCCAGAGAGC ATCCCCAGTC TTGAGGCTCT CACTAGAAAA
GTCCACAACT TCCAGATGGA ATGCTCTCTG CAGGCCAAGC CCGCAGTGCC TACGTCTAAG
CTGGGCCAGG CAGAGGGGCG AGATGCAGGC TCAGCACCCT CCGGAGGGGA CCCGGCCTTC
CTGGGCATGG CCGTGAGCAC CCTGTGTGGC GAGGTGCCGC TCTACTACAT CTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_003009.2
CDS159..752
Misc Feature(1)102..104(+)
Misc Feature(2)159..749(+)
Misc Feature(3)159..437(+)
Misc Feature(4)438..749(+)
Exon (1)1..200
Gene:SFTPC
Gene Synonym:
Exon (2)201..359
Gene:SFTPC
Gene Synonym:
Exon (3)360..482
Gene:SFTPC
Gene Synonym:
Exon (4)483..593
Gene:SFTPC
Gene Synonym:
Exon (5)594..770
Gene:SFTPC
Gene Synonym:
Exon (6)771..984
Gene:SFTPC
Gene Synonym:
Translation
Position Chain Variation Link
25 25 c, g dbSNP:28438700
57 57 a, g dbSNP:751703149
58 58 a, g dbSNP:755444690
59 59 a, g, t dbSNP:74639360
61 61 c, t dbSNP:756529000
64 64 g, t dbSNP:778358826
67 67 c, g dbSNP:79647630
68 68 c, t dbSNP:77253713
70 70 a, t dbSNP:779710215
76 76 c, g dbSNP:746560865
81 81 a, c dbSNP:762050676
82 82 c, g dbSNP:549699699
89 89 c, g dbSNP:750070143
91 91 a, g dbSNP:567738879
92 92 c, t dbSNP:377267823
93 93 a, g dbSNP:367559125
95 95 a, g dbSNP:758318330
102 102 -, aa dbSNP:545529507
102 102 a, t dbSNP:763225526
106 106 a, c, g dbSNP:766625842
107 107 a, c, t dbSNP:373338880
108 108 c, t dbSNP:767971320
115 115 a, c dbSNP:753248473
122 122 -, ggagagga dbSNP:772183067
125 125 g, t dbSNP:756372282
128 128 a, g dbSNP:200665803
133 133 -, gag dbSNP:773257848
141 141 c, t dbSNP:78201008
142 142 a, g dbSNP:758095773
149 149 -, ct dbSNP:760698424
149 149 c, g dbSNP:371755328
156 156 a, c dbSNP:746473012
157 157 a, g dbSNP:768313265
158 158 a, g dbSNP:781209749
168 168 a, g dbSNP:201896861
170 170 a, c dbSNP:564964303
182 182 c, g dbSNP:144603526
184 184 c, t dbSNP:11552815
186 186 a, t dbSNP:773026639
194 194 c, t dbSNP:369413166
197 197 a, c, g dbSNP:532360636
200 200 a, g dbSNP:199905878
201 201 a, g dbSNP:376399274
209 209 c, t dbSNP:752201591
210 210 a, g dbSNP:755609275
212 212 a, g dbSNP:11552816
216 216 c, t dbSNP:777698296
218 218 c, t dbSNP:749232449
220 220 a, g dbSNP:371321234
223 223 a, g dbSNP:778672867
224 224 a, c dbSNP:745700017
225 225 c, t dbSNP:375581850
226 226 a, g, t dbSNP:75902455
231 231 a, g dbSNP:768626980
234 234 a, g dbSNP:368656172
235 235 c, t dbSNP:777139008
238 238 c, t dbSNP:762396958
245 245 c, t dbSNP:770491816
251 251 a, g dbSNP:773682315
252 252 c, t dbSNP:192391655
254 254 c, t dbSNP:767156369
261 261 c, t dbSNP:199854076
272 272 -, gtg dbSNP:762667784
272 272 c, t dbSNP:375940265
273 273 -, gtg dbSNP:752666425
273 273 a, g, t dbSNP:183533911
275 275 -, gtg dbSNP:752783626
288 288 a, g dbSNP:757152791
296 296 c, t dbSNP:548523225
297 297 a, g dbSNP:750172249
299 299 c, g, t dbSNP:764818190
300 300 a, g dbSNP:566914013
302 302 a, g dbSNP:747210099
308 308 g, t dbSNP:768981838
311 311 a, g dbSNP:781417860
315 315 a, g dbSNP:200039720
317 317 c, t dbSNP:376182452
327 327 a, g dbSNP:369239281
334 334 a, g dbSNP:201567623
352 352 c, t dbSNP:547252810
353 353 a, g dbSNP:188074517
354 354 a, g dbSNP:121917836
376 376 a, c, t dbSNP:121917834
383 383 a, g, t dbSNP:369210212
385 385 c, t dbSNP:201454136
386 386 a, c, g dbSNP:75413490
387 387 a, g dbSNP:772841457
399 399 c, t dbSNP:377222742
400 400 a, g dbSNP:766383484
407 407 c, t dbSNP:751292601
414 414 a, g dbSNP:754831645
422 422 a, g dbSNP:370641482
425 425 c, t dbSNP:752977645
434 434 a, c dbSNP:747430307
436 436 c, t dbSNP:777995190
437 437 a, c dbSNP:527723291
442 442 a, c dbSNP:552621627
446 446 c, t dbSNP:79866047
449 449 c, t dbSNP:746180618
452 452 c, t dbSNP:772431756
458 458 c, t dbSNP:377655715
459 459 c, t dbSNP:747785707
461 461 c, t dbSNP:370825102
462 462 a, g dbSNP:772568845
476 476 a, c dbSNP:201933678
486 486 a, c dbSNP:760567018
491 491 c, t dbSNP:764179769
492 492 a, g dbSNP:372529179
497 497 c, t dbSNP:761742367
505 505 a, c dbSNP:121918559
509 509 c, g, t dbSNP:35457216
510 510 c, g dbSNP:758875625
522 522 a, c, t dbSNP:75609798
523 523 a, c dbSNP:77034580
525 525 a, c, g dbSNP:78029713
529 529 c, t dbSNP:755369109
547 547 a, g dbSNP:762396570
557 557 c, t dbSNP:777230179
562 562 -, aggct dbSNP:751323841
565 565 c, t dbSNP:748954723
571 571 a, c dbSNP:4715
572 572 c, t dbSNP:778451328
591 591 c, t dbSNP:745419854
597 597 a, g dbSNP:374909143
601 601 a, g dbSNP:766184622
602 602 c, t dbSNP:751034722
604 604 c, t dbSNP:754481467
612 612 a, g dbSNP:781120153
619 619 c, t dbSNP:752593536
621 621 c, g dbSNP:202145169
625 625 g, t dbSNP:777510956
627 627 c, t dbSNP:749126105
630 630 a, g dbSNP:771089238
632 632 c, g dbSNP:373783536
638 638 a, g dbSNP:745893171
648 648 a, g dbSNP:371013652
656 656 a, g dbSNP:775696772
658 658 a, g dbSNP:34957318
666 666 a, g dbSNP:769328550
670 670 c, t dbSNP:777114058
678 678 a, t dbSNP:546357906
681 681 a, g dbSNP:766093799
685 685 a, g dbSNP:537383138
691 691 c, t dbSNP:759031390
692 692 a, g dbSNP:149606090
693 693 c, g dbSNP:752289567
694 694 c, t dbSNP:531634308
699 699 c, g dbSNP:201685063
705 705 a, g dbSNP:777707126
706 706 g, t dbSNP:753589128
708 708 a, g dbSNP:757050514
710 710 c, t dbSNP:779136923
715 715 a, g dbSNP:1124
721 721 a, t dbSNP:121917835
723 723 c, t dbSNP:76821562
728 728 c, t dbSNP:529959941
729 729 a, g dbSNP:200839715
731 731 a, c, g dbSNP:747175730
733 733 c, t dbSNP:769238316
739 739 c, t dbSNP:121918560
749 749 c, t dbSNP:3203602
751 751 a, g dbSNP:777312443
752 752 c, g dbSNP:370068089
759 759 g, t dbSNP:566370122
762 762 a, g dbSNP:773687273
764 764 a, c, t dbSNP:200469074
768 768 a, c, g dbSNP:12229
780 780 a, g dbSNP:1804453
788 788 a, g dbSNP:75518353
792 792 g, t dbSNP:543423602
807 807 a, g dbSNP:748654127
863 863 c, g dbSNP:565381858
864 864 c, t dbSNP:562115512
866 866 c, t dbSNP:11552817
875 875 a, g dbSNP:7592
881 881 a, g dbSNP:749681094
891 891 a, g dbSNP:541769233
925 925 c, t dbSNP:1126931
926 926 a, g, t dbSNP:1139547
929 929 c, t dbSNP:552366898
933 933 a, g dbSNP:763580777
934 934 c, g, t dbSNP:8192331
944 944 c, t dbSNP:776190058
950 950 a, c dbSNP:761708440
968 968 c, t dbSNP:8192342
973 973 c, t dbSNP:765269411

Target ORF information:

RefSeq Version NM_003018
Organism Homo sapiens (human)
Definition Homo sapiens surfactant protein C (SFTPC), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003018

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGATGTGG GCAGCAAAGA GGTCCTGATG GAGAGCCCGC CGGACTACTC CGCAGCTCCC 
CGGGGCCGAT TTGGCATTCC CTGCTGCCCA GTGCACCTGA AACGCCTTCT TATCGTGGTG
GTGGTGGTGG TCCTCATCGT CGTGGTGATT GTGGGAGCCC TGCTCATGGG TCTCCACATG
AGCCAGAAAC ACACGGAGAT GGTTCTGGAG ATGAGCATTG GGGCGCCGGA AGCCCAGCAA
CGCCTGGCCC TGAGTGAGCA CCTGGTTACC ACTGCCACCT TCTCCATCGG CTCCACTGGC
CTCGTGGTGT ATGACTACCA GCAGCTGCTG ATCGCCTACA AGCCAGCCCC TGGCACCTGC
TGCTACATCA TGAAGATAGC TCCAGAGAGC ATCCCCAGTC TTGAGGCTCT CACTAGAAAA
GTCCACAACT TCCAGATGGA ATGCTCTCTG CAGGCCAAGC CCGCAGTGCC TACGTCTAAG
CTGGGCCAGG CAGAGGGGCG AGATGCAGGC TCAGCACCCT CCGGAGGGGA CCCGGCCTTC
CTGGGCATGG CCGTGAGCAC CCTGTGTGGC GAGGTGCCGC TCTACTACAT CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22858
Accession Version NM_001172357.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 576bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pulmonary surfactant-associated protein C isoform 2 proprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J03553.1, BC105594.1 and BM993053.1. Summary: This gene encodes the pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 2, also called pulmonary alveolar proteinosis due to surfactant protein C deficiency, and are associated with interstitial lung disease in older infants, children, and adults. Alternatively spliced transcript variants encoding different protein isoforms have been identified.[provided by RefSeq, Feb 2010]. Transcript Variant: This variant (3) lacks an in-frame segment in the 3' coding region and has an additional segment in the 3' UTR, as compared to variant 1. The transcript is longer than variant 1, but encodes a shorter isoform (2), as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC105594.1, BG542819.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
ATGGATGTGG GCAGCAAAGA GGTCCTGATG GAGAGCCCGC CGGACTACTC CGCAGCTCCC 
CGGGGCCGAT TTGGCATTCC CTGCTGCCCA GTGCACCTGA AACGCCTTCT TATCGTGGTG
GTGGTGGTGG TCCTCATCGT CGTGGTGATT GTGGGAGCCC TGCTCATGGG TCTCCACATG
AGCCAGAAAC ACACGGAGAT GGTTCTGGAG ATGAGCATTG GGGCGCCGGA AGCCCAGCAA
CGCCTGGCCC TGAGTGAGCA CCTGGTTACC ACTGCCACCT TCTCCATCGG CTCCACTGGC
CTCGTGGTGT ATGACTACCA GCAGCTGCTG ATCGCCTACA AGCCAGCCCC TGGCACCTGC
TGCTACATCA TGAAGATAGC TCCAGAGAGC ATCCCCAGTC TTGAGGCTCT CACTAGAAAA
GTCCACAACT TCCAGGCCAA GCCCGCAGTG CCTACGTCTA AGCTGGGCCA GGCAGAGGGG
CGAGATGCAG GCTCAGCACC CTCCGGAGGG GACCCGGCCT TCCTGGGCAT GGCCGTGAGC
ACCCTGTGTG GCGAGGTGCC GCTCTACTAC ATCTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001165828.1
CDS159..734
Misc Feature(1)102..104(+)
Misc Feature(2)159..731(+)
Misc Feature(3)159..437(+)
Misc Feature(4)438..731(+)
Exon (1)1..200
Gene:SFTPC
Gene Synonym:
Exon (2)201..359
Gene:SFTPC
Gene Synonym:
Exon (3)360..482
Gene:SFTPC
Gene Synonym:
Exon (4)483..593
Gene:SFTPC
Gene Synonym:
Exon (5)594..1172
Gene:SFTPC
Gene Synonym:
Translation
Position Chain Variation Link
25 25 c, g dbSNP:28438700
57 57 a, g dbSNP:751703149
58 58 a, g dbSNP:755444690
59 59 a, g, t dbSNP:74639360
61 61 c, t dbSNP:756529000
64 64 g, t dbSNP:778358826
67 67 c, g dbSNP:79647630
68 68 c, t dbSNP:77253713
70 70 a, t dbSNP:779710215
76 76 c, g dbSNP:746560865
81 81 a, c dbSNP:762050676
82 82 c, g dbSNP:549699699
89 89 c, g dbSNP:750070143
91 91 a, g dbSNP:567738879
92 92 c, t dbSNP:377267823
93 93 a, g dbSNP:367559125
95 95 a, g dbSNP:758318330
102 102 -, aa dbSNP:545529507
102 102 a, t dbSNP:763225526
106 106 a, c, g dbSNP:766625842
107 107 a, c, t dbSNP:373338880
108 108 c, t dbSNP:767971320
115 115 a, c dbSNP:753248473
122 122 -, ggagagga dbSNP:772183067
125 125 g, t dbSNP:756372282
128 128 a, g dbSNP:200665803
133 133 -, gag dbSNP:773257848
141 141 c, t dbSNP:78201008
142 142 a, g dbSNP:758095773
149 149 -, ct dbSNP:760698424
149 149 c, g dbSNP:371755328
156 156 a, c dbSNP:746473012
157 157 a, g dbSNP:768313265
158 158 a, g dbSNP:781209749
168 168 a, g dbSNP:201896861
170 170 a, c dbSNP:564964303
182 182 c, g dbSNP:144603526
184 184 c, t dbSNP:11552815
186 186 a, t dbSNP:773026639
194 194 c, t dbSNP:369413166
197 197 a, c, g dbSNP:532360636
200 200 a, g dbSNP:199905878
201 201 a, g dbSNP:376399274
209 209 c, t dbSNP:752201591
210 210 a, g dbSNP:755609275
212 212 a, g dbSNP:11552816
216 216 c, t dbSNP:777698296
218 218 c, t dbSNP:749232449
220 220 a, g dbSNP:371321234
223 223 a, g dbSNP:778672867
224 224 a, c dbSNP:745700017
225 225 c, t dbSNP:375581850
226 226 a, g, t dbSNP:75902455
231 231 a, g dbSNP:768626980
234 234 a, g dbSNP:368656172
235 235 c, t dbSNP:777139008
238 238 c, t dbSNP:762396958
245 245 c, t dbSNP:770491816
251 251 a, g dbSNP:773682315
252 252 c, t dbSNP:192391655
254 254 c, t dbSNP:767156369
261 261 c, t dbSNP:199854076
272 272 -, gtg dbSNP:762667784
272 272 c, t dbSNP:375940265
273 273 -, gtg dbSNP:752666425
273 273 a, g, t dbSNP:183533911
275 275 -, gtg dbSNP:752783626
288 288 a, g dbSNP:757152791
296 296 c, t dbSNP:548523225
297 297 a, g dbSNP:750172249
299 299 c, g, t dbSNP:764818190
300 300 a, g dbSNP:566914013
302 302 a, g dbSNP:747210099
308 308 g, t dbSNP:768981838
311 311 a, g dbSNP:781417860
315 315 a, g dbSNP:200039720
317 317 c, t dbSNP:376182452
327 327 a, g dbSNP:369239281
334 334 a, g dbSNP:201567623
352 352 c, t dbSNP:547252810
353 353 a, g dbSNP:188074517
354 354 a, g dbSNP:121917836
376 376 a, c, t dbSNP:121917834
383 383 a, g, t dbSNP:369210212
385 385 c, t dbSNP:201454136
386 386 a, c, g dbSNP:75413490
387 387 a, g dbSNP:772841457
399 399 c, t dbSNP:377222742
400 400 a, g dbSNP:766383484
407 407 c, t dbSNP:751292601
414 414 a, g dbSNP:754831645
422 422 a, g dbSNP:370641482
425 425 c, t dbSNP:752977645
434 434 a, c dbSNP:747430307
436 436 c, t dbSNP:777995190
437 437 a, c dbSNP:527723291
442 442 a, c dbSNP:552621627
446 446 c, t dbSNP:79866047
449 449 c, t dbSNP:746180618
452 452 c, t dbSNP:772431756
458 458 c, t dbSNP:377655715
459 459 c, t dbSNP:747785707
461 461 c, t dbSNP:370825102
462 462 a, g dbSNP:772568845
476 476 a, c dbSNP:201933678
486 486 a, c dbSNP:760567018
491 491 c, t dbSNP:764179769
492 492 a, g dbSNP:372529179
497 497 c, t dbSNP:761742367
505 505 a, c dbSNP:121918559
509 509 c, g, t dbSNP:35457216
510 510 c, g dbSNP:758875625
522 522 a, c, t dbSNP:75609798
523 523 a, c dbSNP:77034580
525 525 a, c, g dbSNP:78029713
529 529 c, t dbSNP:755369109
547 547 a, g dbSNP:762396570
557 557 c, t dbSNP:777230179
562 562 -, aggct dbSNP:751323841
565 565 c, t dbSNP:748954723
571 571 a, c dbSNP:4715
572 572 c, t dbSNP:778451328
591 591 c, t dbSNP:745419854
594 594 a, g dbSNP:781120153
601 601 c, t dbSNP:752593536
603 603 c, g dbSNP:202145169
607 607 g, t dbSNP:777510956
609 609 c, t dbSNP:749126105
612 612 a, g dbSNP:771089238
614 614 c, g dbSNP:373783536
620 620 a, g dbSNP:745893171
630 630 a, g dbSNP:371013652
638 638 a, g dbSNP:775696772
640 640 a, g dbSNP:34957318
648 648 a, g dbSNP:769328550
652 652 c, t dbSNP:777114058
660 660 a, t dbSNP:546357906
663 663 a, g dbSNP:766093799
667 667 a, g dbSNP:537383138
673 673 c, t dbSNP:759031390
674 674 a, g dbSNP:149606090
675 675 c, g dbSNP:752289567
676 676 c, t dbSNP:531634308
681 681 c, g dbSNP:201685063
687 687 a, g dbSNP:777707126
688 688 g, t dbSNP:753589128
690 690 a, g dbSNP:757050514
692 692 c, t dbSNP:779136923
697 697 a, g dbSNP:1124
703 703 a, t dbSNP:121917835
705 705 c, t dbSNP:76821562
710 710 c, t dbSNP:529959941
711 711 a, g dbSNP:200839715
713 713 a, c, g dbSNP:747175730
715 715 c, t dbSNP:769238316
721 721 c, t dbSNP:121918560
731 731 c, t dbSNP:3203602
733 733 a, g dbSNP:777312443
734 734 c, g dbSNP:370068089
741 741 g, t dbSNP:566370122
744 744 a, g dbSNP:773687273
746 746 a, c, t dbSNP:200469074
750 750 a, c, g dbSNP:12229
753 753 a, g dbSNP:752095125
764 764 a, g dbSNP:202194863
765 765 a, g dbSNP:74515418
767 767 a, c dbSNP:753786425
770 770 c, t dbSNP:375538518
772 772 a, g dbSNP:777132226
779 779 a, g dbSNP:750273486
781 781 c, t dbSNP:377337781
782 782 a, g dbSNP:758529805
784 784 a, c dbSNP:780345754
786 786 a, g dbSNP:747047170
790 790 c, g dbSNP:370783709
800 800 c, g dbSNP:568615387
807 807 a, g dbSNP:189992977
865 865 a, t dbSNP:181622448
869 869 a, g dbSNP:573006492
905 905 a, c dbSNP:187232702
911 911 a, t dbSNP:546294880
919 919 -, a dbSNP:3216166
920 920 -, a dbSNP:397797745
922 922 -, tctc dbSNP:779309006
936 936 c, t dbSNP:558003013
944 944 c, t dbSNP:767551791
956 956 c, t dbSNP:1126948
968 968 a, g dbSNP:1804453
976 976 a, g dbSNP:75518353
980 980 g, t dbSNP:543423602
995 995 a, g dbSNP:748654127
1051 1051 c, g dbSNP:565381858
1052 1052 c, t dbSNP:562115512
1054 1054 c, t dbSNP:11552817
1063 1063 a, g dbSNP:7592
1069 1069 a, g dbSNP:749681094
1079 1079 a, g dbSNP:541769233
1113 1113 c, t dbSNP:1126931
1114 1114 a, g, t dbSNP:1139547
1117 1117 c, t dbSNP:552366898
1121 1121 a, g dbSNP:763580777
1122 1122 c, g, t dbSNP:8192331
1132 1132 c, t dbSNP:776190058
1138 1138 a, c dbSNP:761708440
1156 1156 c, t dbSNP:8192342
1161 1161 c, t dbSNP:765269411

Target ORF information:

RefSeq Version NM_001172357
Organism Homo sapiens (human)
Definition Homo sapiens surfactant protein C (SFTPC), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001172357

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGATGTGG GCAGCAAAGA GGTCCTGATG GAGAGCCCGC CGGACTACTC CGCAGCTCCC 
CGGGGCCGAT TTGGCATTCC CTGCTGCCCA GTGCACCTGA AACGCCTTCT TATCGTGGTG
GTGGTGGTGG TCCTCATCGT CGTGGTGATT GTGGGAGCCC TGCTCATGGG TCTCCACATG
AGCCAGAAAC ACACGGAGAT GGTTCTGGAG ATGAGCATTG GGGCGCCGGA AGCCCAGCAA
CGCCTGGCCC TGAGTGAGCA CCTGGTTACC ACTGCCACCT TCTCCATCGG CTCCACTGGC
CTCGTGGTGT ATGACTACCA GCAGCTGCTG ATCGCCTACA AGCCAGCCCC TGGCACCTGC
TGCTACATCA TGAAGATAGC TCCAGAGAGC ATCCCCAGTC TTGAGGCTCT CACTAGAAAA
GTCCACAACT TCCAGGCCAA GCCCGCAGTG CCTACGTCTA AGCTGGGCCA GGCAGAGGGG
CGAGATGCAG GCTCAGCACC CTCCGGAGGG GACCCGGCCT TCCTGGGCAT GGCCGTGAGC
ACCCTGTGTG GCGAGGTGCC GCTCTACTAC ATCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22601
Accession Version NM_001172410.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 594bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product pulmonary surfactant-associated protein C isoform 1 proprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from J03553.1, BC005913.1, AK315742.1 and BQ027183.1. Summary: This gene encodes the pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 2, also called pulmonary alveolar proteinosis due to surfactant protein C deficiency, and are associated with interstitial lung disease in older infants, children, and adults. Alternatively spliced transcript variants encoding different protein isoforms have been identified.[provided by RefSeq, Feb 2010]. Transcript Variant: This variant (2) lacks an internal segment in the 3' UTR, as compared to variant 1. Both variants 1 and 2 encode the same isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005913.1, BG485480.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2142363 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
ATGGATGTGG GCAGCAAAGA GGTCCTGATG GAGAGCCCGC CGGACTACTC CGCAGCTCCC 
CGGGGCCGAT TTGGCATTCC CTGCTGCCCA GTGCACCTGA AACGCCTTCT TATCGTGGTG
GTGGTGGTGG TCCTCATCGT CGTGGTGATT GTGGGAGCCC TGCTCATGGG TCTCCACATG
AGCCAGAAAC ACACGGAGAT GGTTCTGGAG ATGAGCATTG GGGCGCCGGA AGCCCAGCAA
CGCCTGGCCC TGAGTGAGCA CCTGGTTACC ACTGCCACCT TCTCCATCGG CTCCACTGGC
CTCGTGGTGT ATGACTACCA GCAGCTGCTG ATCGCCTACA AGCCAGCCCC TGGCACCTGC
TGCTACATCA TGAAGATAGC TCCAGAGAGC ATCCCCAGTC TTGAGGCTCT CACTAGAAAA
GTCCACAACT TCCAGATGGA ATGCTCTCTG CAGGCCAAGC CCGCAGTGCC TACGTCTAAG
CTGGGCCAGG CAGAGGGGCG AGATGCAGGC TCAGCACCCT CCGGAGGGGA CCCGGCCTTC
CTGGGCATGG CCGTGAGCAC CCTGTGTGGC GAGGTGCCGC TCTACTACAT CTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001165881.1
CDS159..752
Misc Feature(1)102..104(+)
Misc Feature(2)159..749(+)
Misc Feature(3)159..437(+)
Misc Feature(4)438..749(+)
Exon (1)1..200
Gene:SFTPC
Gene Synonym:
Exon (2)201..359
Gene:SFTPC
Gene Synonym:
Exon (3)360..482
Gene:SFTPC
Gene Synonym:
Exon (4)483..593
Gene:SFTPC
Gene Synonym:
Exon (5)594..762
Gene:SFTPC
Gene Synonym:
Exon (6)763..976
Gene:SFTPC
Gene Synonym:
Translation
Position Chain Variation Link
25 25 c, g dbSNP:28438700
57 57 a, g dbSNP:751703149
58 58 a, g dbSNP:755444690
59 59 a, g, t dbSNP:74639360
61 61 c, t dbSNP:756529000
64 64 g, t dbSNP:778358826
67 67 c, g dbSNP:79647630
68 68 c, t dbSNP:77253713
70 70 a, t dbSNP:779710215
76 76 c, g dbSNP:746560865
81 81 a, c dbSNP:762050676
82 82 c, g dbSNP:549699699
89 89 c, g dbSNP:750070143
91 91 a, g dbSNP:567738879
92 92 c, t dbSNP:377267823
93 93 a, g dbSNP:367559125
95 95 a, g dbSNP:758318330
102 102 -, aa dbSNP:545529507
102 102 a, t dbSNP:763225526
106 106 a, c, g dbSNP:766625842
107 107 a, c, t dbSNP:373338880
108 108 c, t dbSNP:767971320
115 115 a, c dbSNP:753248473
122 122 -, ggagagga dbSNP:772183067
125 125 g, t dbSNP:756372282
128 128 a, g dbSNP:200665803
133 133 -, gag dbSNP:773257848
141 141 c, t dbSNP:78201008
142 142 a, g dbSNP:758095773
149 149 -, ct dbSNP:760698424
149 149 c, g dbSNP:371755328
156 156 a, c dbSNP:746473012
157 157 a, g dbSNP:768313265
158 158 a, g dbSNP:781209749
168 168 a, g dbSNP:201896861
170 170 a, c dbSNP:564964303
182 182 c, g dbSNP:144603526
184 184 c, t dbSNP:11552815
186 186 a, t dbSNP:773026639
194 194 c, t dbSNP:369413166
197 197 a, c, g dbSNP:532360636
200 200 a, g dbSNP:199905878
201 201 a, g dbSNP:376399274
209 209 c, t dbSNP:752201591
210 210 a, g dbSNP:755609275
212 212 a, g dbSNP:11552816
216 216 c, t dbSNP:777698296
218 218 c, t dbSNP:749232449
220 220 a, g dbSNP:371321234
223 223 a, g dbSNP:778672867
224 224 a, c dbSNP:745700017
225 225 c, t dbSNP:375581850
226 226 a, g, t dbSNP:75902455
231 231 a, g dbSNP:768626980
234 234 a, g dbSNP:368656172
235 235 c, t dbSNP:777139008
238 238 c, t dbSNP:762396958
245 245 c, t dbSNP:770491816
251 251 a, g dbSNP:773682315
252 252 c, t dbSNP:192391655
254 254 c, t dbSNP:767156369
261 261 c, t dbSNP:199854076
272 272 -, gtg dbSNP:762667784
272 272 c, t dbSNP:375940265
273 273 -, gtg dbSNP:752666425
273 273 a, g, t dbSNP:183533911
275 275 -, gtg dbSNP:752783626
288 288 a, g dbSNP:757152791
296 296 c, t dbSNP:548523225
297 297 a, g dbSNP:750172249
299 299 c, g, t dbSNP:764818190
300 300 a, g dbSNP:566914013
302 302 a, g dbSNP:747210099
308 308 g, t dbSNP:768981838
311 311 a, g dbSNP:781417860
315 315 a, g dbSNP:200039720
317 317 c, t dbSNP:376182452
327 327 a, g dbSNP:369239281
334 334 a, g dbSNP:201567623
352 352 c, t dbSNP:547252810
353 353 a, g dbSNP:188074517
354 354 a, g dbSNP:121917836
376 376 a, c, t dbSNP:121917834
383 383 a, g, t dbSNP:369210212
385 385 c, t dbSNP:201454136
386 386 a, c, g dbSNP:75413490
387 387 a, g dbSNP:772841457
399 399 c, t dbSNP:377222742
400 400 a, g dbSNP:766383484
407 407 c, t dbSNP:751292601
414 414 a, g dbSNP:754831645
422 422 a, g dbSNP:370641482
425 425 c, t dbSNP:752977645
434 434 a, c dbSNP:747430307
436 436 c, t dbSNP:777995190
437 437 a, c dbSNP:527723291
442 442 a, c dbSNP:552621627
446 446 c, t dbSNP:79866047
449 449 c, t dbSNP:746180618
452 452 c, t dbSNP:772431756
458 458 c, t dbSNP:377655715
459 459 c, t dbSNP:747785707
461 461 c, t dbSNP:370825102
462 462 a, g dbSNP:772568845
476 476 a, c dbSNP:201933678
486 486 a, c dbSNP:760567018
491 491 c, t dbSNP:764179769
492 492 a, g dbSNP:372529179
497 497 c, t dbSNP:761742367
505 505 a, c dbSNP:121918559
509 509 c, g, t dbSNP:35457216
510 510 c, g dbSNP:758875625
522 522 a, c, t dbSNP:75609798
523 523 a, c dbSNP:77034580
525 525 a, c, g dbSNP:78029713
529 529 c, t dbSNP:755369109
547 547 a, g dbSNP:762396570
557 557 c, t dbSNP:777230179
562 562 -, aggct dbSNP:751323841
565 565 c, t dbSNP:748954723
571 571 a, c dbSNP:4715
572 572 c, t dbSNP:778451328
591 591 c, t dbSNP:745419854
597 597 a, g dbSNP:374909143
601 601 a, g dbSNP:766184622
602 602 c, t dbSNP:751034722
604 604 c, t dbSNP:754481467
612 612 a, g dbSNP:781120153
619 619 c, t dbSNP:752593536
621 621 c, g dbSNP:202145169
625 625 g, t dbSNP:777510956
627 627 c, t dbSNP:749126105
630 630 a, g dbSNP:771089238
632 632 c, g dbSNP:373783536
638 638 a, g dbSNP:745893171
648 648 a, g dbSNP:371013652
656 656 a, g dbSNP:775696772
658 658 a, g dbSNP:34957318
666 666 a, g dbSNP:769328550
670 670 c, t dbSNP:777114058
678 678 a, t dbSNP:546357906
681 681 a, g dbSNP:766093799
685 685 a, g dbSNP:537383138
691 691 c, t dbSNP:759031390
692 692 a, g dbSNP:149606090
693 693 c, g dbSNP:752289567
694 694 c, t dbSNP:531634308
699 699 c, g dbSNP:201685063
705 705 a, g dbSNP:777707126
706 706 g, t dbSNP:753589128
708 708 a, g dbSNP:757050514
710 710 c, t dbSNP:779136923
715 715 a, g dbSNP:1124
721 721 a, t dbSNP:121917835
723 723 c, t dbSNP:76821562
728 728 c, t dbSNP:529959941
729 729 a, g dbSNP:200839715
731 731 a, c, g dbSNP:747175730
733 733 c, t dbSNP:769238316
739 739 c, t dbSNP:121918560
749 749 c, t dbSNP:3203602
751 751 a, g dbSNP:777312443
752 752 c, g dbSNP:370068089
759 759 g, t dbSNP:566370122
762 762 a, g dbSNP:773687273
772 772 a, g dbSNP:1804453
780 780 a, g dbSNP:75518353
784 784 g, t dbSNP:543423602
799 799 a, g dbSNP:748654127
855 855 c, g dbSNP:565381858
856 856 c, t dbSNP:562115512
858 858 c, t dbSNP:11552817
867 867 a, g dbSNP:7592
873 873 a, g dbSNP:749681094
883 883 a, g dbSNP:541769233
917 917 c, t dbSNP:1126931
918 918 a, g, t dbSNP:1139547
921 921 c, t dbSNP:552366898
925 925 a, g dbSNP:763580777
926 926 c, g, t dbSNP:8192331
936 936 c, t dbSNP:776190058
942 942 a, c dbSNP:761708440
960 960 c, t dbSNP:8192342
965 965 c, t dbSNP:765269411

Target ORF information:

RefSeq Version NM_001172410
Organism Homo sapiens (human)
Definition Homo sapiens surfactant protein C (SFTPC), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001172410

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGATGTGG GCAGCAAAGA GGTCCTGATG GAGAGCCCGC CGGACTACTC CGCAGCTCCC 
CGGGGCCGAT TTGGCATTCC CTGCTGCCCA GTGCACCTGA AACGCCTTCT TATCGTGGTG
GTGGTGGTGG TCCTCATCGT CGTGGTGATT GTGGGAGCCC TGCTCATGGG TCTCCACATG
AGCCAGAAAC ACACGGAGAT GGTTCTGGAG ATGAGCATTG GGGCGCCGGA AGCCCAGCAA
CGCCTGGCCC TGAGTGAGCA CCTGGTTACC ACTGCCACCT TCTCCATCGG CTCCACTGGC
CTCGTGGTGT ATGACTACCA GCAGCTGCTG ATCGCCTACA AGCCAGCCCC TGGCACCTGC
TGCTACATCA TGAAGATAGC TCCAGAGAGC ATCCCCAGTC TTGAGGCTCT CACTAGAAAA
GTCCACAACT TCCAGATGGA ATGCTCTCTG CAGGCCAAGC CCGCAGTGCC TACGTCTAAG
CTGGGCCAGG CAGAGGGGCG AGATGCAGGC TCAGCACCCT CCGGAGGGGA CCCGGCCTTC
CTGGGCATGG CCGTGAGCAC CCTGTGTGGC GAGGTGCCGC TCTACTACAT CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.