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SGSH cDNA ORF clone, Homo sapiens (human)

Gene Symbol SGSH
Entrez Gene ID 6448
Full Name N-sulfoglucosamine sulfohydrolase
Synonyms HSS, MPS3A, SFMD
General protein information
Preferred Names
N-sulphoglucosamine sulphohydrolase
Names
N-sulphoglucosamine sulphohydrolase
sulphamidase
heparan sulfate sulfatase
sulfoglucosamine sulfamidase
mucopolysaccharidosis type IIIA
NP_000190.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q25.3

Summary This gene encodes one of several enzymes involved in the lysosomal degradation of heparan sulfate. Mutations in this gene are associated with Sanfilippo syndrome A, one type of the lysosomal storage disease mucopolysaccaridosis III, which results from impaired degradation of heparan sulfate. Transcripts of varying sizes have been reported but their biological validity has not been determined. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

605270

Disorder Html: Mucopolysaccharidisis type IIIA (Sanfilippo A), 252900 (3)





The following SGSH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SGSH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu50585 XM_005257582 PREDICTED: Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu50586 XM_005257583 PREDICTED: Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu60463 XM_011525126 PREDICTED: Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu60464 XM_011525127 PREDICTED: Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu18039 NM_000199 Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$265.30
$379.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu50585
Accession Version XM_005257582.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1089bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product N-sulphoglucosamine sulphohydrolase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530412677. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGG CACTGAGGCC
CAGAGAGATG ACGTGGCGGT GAGAGGTGTC GGCTCGACAA GGCACCTCCC GCTGCTGATT
GGATTGGAGA AGGGAGCCGC CCAGAGTGGC GTAGGTTCGG CCCTTCCGAG GGCAGCTCCT
GTGTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_005257639.1
CDS21..1109
Misc Feature(1)78..>968(+)
Misc Feature(2)87..962(+)
Translation
Position Chain Variation Link
4 4 c, t dbSNP:751812573
5 5 c, t dbSNP:566268948
38 38 c, g dbSNP:544042160
39 39 a, g dbSNP:548145029
56 56 c, g dbSNP:777433840
87 87 c, t dbSNP:529571746
96 96 c, t dbSNP:755001766
103 103 -, gct dbSNP:786200990
105 105 a, c dbSNP:562218609
108 108 c, g dbSNP:758793075
109 109 c, t dbSNP:767227727
110 110 a, g dbSNP:549213151
116 116 c, t dbSNP:139850991
117 117 a, g dbSNP:398123246
118 118 a, g dbSNP:762732038
135 135 a, g dbSNP:773137508
136 136 c, t dbSNP:372283734
137 137 a, g dbSNP:201132655
140 140 a, c dbSNP:562933313
141 141 a, g dbSNP:768751214
145 145 -, aca dbSNP:746784710
149 149 c, t dbSNP:747247188
155 155 c, t dbSNP:775770560
156 156 a, g dbSNP:772636496
158 158 c, t dbSNP:746172006
163 163 c, t dbSNP:779581338
164 164 a, g dbSNP:145970971
165 165 c, t dbSNP:749768976
167 167 c, t dbSNP:780614886
168 168 c, g dbSNP:376752886
173 173 c, t dbSNP:372226239
174 174 a, g dbSNP:551094331
175 175 c, t dbSNP:758203457
183 183 c, t dbSNP:368650877
184 184 a, g dbSNP:558991918
186 186 c, t dbSNP:761780038
189 189 a, g dbSNP:753795068
201 201 c, t dbSNP:142309764
202 202 a, g, t dbSNP:573342437
203 203 c, t dbSNP:561475181
209 209 c, g dbSNP:760006618
217 217 c, g, t dbSNP:104894637
218 218 a, g dbSNP:771397375
231 231 c, t dbSNP:398123245
240 240 c, t dbSNP:104894636
241 241 a, g dbSNP:778336949
242 242 c, t dbSNP:768056322
243 243 a, g dbSNP:137953179
255 255 a, c dbSNP:779703983
257 257 c, t dbSNP:758007069
258 258 a, g dbSNP:750048841
261 261 c, t dbSNP:778730409
263 263 a, g, t dbSNP:543297521
270 270 -, catcag dbSNP:757278903
288 288 a, g dbSNP:774010006
296 296 c, g dbSNP:201478799
302 302 c, t dbSNP:748857638
312 312 c, t dbSNP:777669364
317 317 c, g dbSNP:755846091
323 323 c, t dbSNP:150482611
324 324 a, g dbSNP:781207515
328 328 a, g dbSNP:755122392
329 329 a, g dbSNP:751641089
330 330 a, g dbSNP:766542212
333 333 c, t dbSNP:375755239
334 334 a, g dbSNP:375058146
335 335 c, g dbSNP:765693219
343 343 c, t dbSNP:762164487
344 344 a, g dbSNP:147938702
347 347 a, g dbSNP:764531454
350 350 a, g dbSNP:547771707
355 355 c, g dbSNP:773947080
364 364 g, t dbSNP:770321343
367 367 c, g, t dbSNP:148347556
369 369 c, t dbSNP:772732363
370 370 a, g dbSNP:769633955
371 371 c, t dbSNP:747892803
373 373 c, t dbSNP:781299370
384 384 a, g dbSNP:761607612
386 386 a, g dbSNP:776410862
395 395 c, t dbSNP:774482734
396 396 a, c, g dbSNP:775553760
398 398 g, t dbSNP:771878830
399 399 c, g dbSNP:745996666
403 403 c, t dbSNP:104894642
404 404 a, g dbSNP:770944273
410 410 c, g, t dbSNP:111761143
411 411 a, g dbSNP:370636303
417 417 a, c dbSNP:199643693
418 418 c, t dbSNP:781743238
419 419 a, c, g dbSNP:752056541
426 426 a, t dbSNP:764852930
427 427 -, tt dbSNP:754004591
430 430 c, t dbSNP:367805575
431 431 a, g dbSNP:142557761
435 435 a, g, t dbSNP:760564328
436 436 c, t dbSNP:775112689
437 437 a, g dbSNP:142729872
443 443 c, g dbSNP:769601150
451 451 c, t dbSNP:759410190
452 452 c, t dbSNP:200194908
453 453 a, g dbSNP:771068876
457 457 c, t dbSNP:749358773
466 466 g, t dbSNP:773474993
469 469 a, g dbSNP:104894638
475 475 c, t dbSNP:748362261
476 476 c, g dbSNP:781433391
479 479 c, t dbSNP:755311312
481 481 -, t dbSNP:36003850
485 485 g, t dbSNP:747521615
488 488 g, t dbSNP:780700374
489 489 c, g dbSNP:535751912
494 494 c, g, t dbSNP:75828254
495 495 a, g dbSNP:755749619
498 498 c, t dbSNP:189571889
499 499 a, g dbSNP:767224326
500 500 -, t dbSNP:34014912
506 506 c, t dbSNP:371061954
523 523 a, c dbSNP:774333201
525 525 c, t dbSNP:770108057
528 528 c, g dbSNP:527760024
536 536 c, t dbSNP:758123603
539 539 c, t dbSNP:750394348
540 540 c, t dbSNP:765206157
542 542 c, t dbSNP:147064455
543 543 a, g dbSNP:753921560
546 546 a, g dbSNP:141533664
547 547 c, g dbSNP:760996554
548 548 a, c dbSNP:775792252
554 554 c, t dbSNP:139484283
555 555 a, c, g dbSNP:774773010
557 557 c, t dbSNP:771440037
560 560 c, g dbSNP:749721134
562 562 a, g dbSNP:776228545
564 564 c, t dbSNP:529855742
565 565 a, g dbSNP:372911015
576 576 a, t dbSNP:779677047
581 581 g, t dbSNP:758246399
583 583 c, t dbSNP:745651937
587 587 a, g dbSNP:778755182
590 590 c, t dbSNP:149951602
591 591 a, g dbSNP:753666460
594 594 a, g dbSNP:764278674
605 605 c, g dbSNP:756193095
617 617 c, t dbSNP:753030382
618 618 a, g dbSNP:767834681
626 626 c, t dbSNP:544509805
627 627 a, c, g dbSNP:369368791
631 631 c, t dbSNP:766605672
636 636 c, t dbSNP:532180050
637 637 a, c, g dbSNP:104894643
650 650 a, g dbSNP:373031930
652 652 c, t dbSNP:746508510
653 653 a, c dbSNP:187147954
656 656 c, t dbSNP:771680187
657 657 a, c dbSNP:200644359
661 661 a, c dbSNP:74000629
663 663 c, t dbSNP:756939944
665 665 c, t dbSNP:151227799
666 666 a, g dbSNP:777843748
677 677 c, t dbSNP:756315850
678 678 a, c, g, t dbSNP:150508741
681 681 c, t dbSNP:751677255
688 688 c, t dbSNP:760542684
690 690 -, t dbSNP:766980170
695 695 c, g, t dbSNP:34520362
696 696 a, g dbSNP:145967352
700 700 c, g dbSNP:774602372
703 703 a, g dbSNP:771177142
704 704 c, t dbSNP:562717448
707 707 a, c dbSNP:778017231
708 708 c, t dbSNP:756437160
709 709 c, t dbSNP:748671633
710 710 a, g dbSNP:781715169
711 711 -, gcagcccg dbSNP:761216892
717 717 c, g, t dbSNP:374621913
718 718 a, g dbSNP:370291256
721 721 c, g dbSNP:113641837
723 723 a, g dbSNP:753472891
724 724 a, t dbSNP:763800418
730 730 c, t dbSNP:760510972
732 732 a, g dbSNP:752632460
733 733 c, t dbSNP:767416947
740 740 c, t dbSNP:367654527
742 742 c, t dbSNP:774439487
747 747 a, g dbSNP:770944322
749 749 a, c, t dbSNP:17854435
750 750 a, g dbSNP:773480617
754 754 a, g dbSNP:104894635
769 769 c, t dbSNP:765180942
771 771 a, g dbSNP:528633031
772 772 c, g dbSNP:144461610
777 777 -, g dbSNP:760281672
793 793 a, g dbSNP:549606472
797 797 c, t dbSNP:761104676
798 798 a, g dbSNP:776183743
800 800 c, t dbSNP:530964770
801 801 a, g dbSNP:149248428
812 812 c, t dbSNP:147962306
817 817 c, t dbSNP:769121533
818 818 a, g dbSNP:199562202
826 826 a, t dbSNP:780812356
828 828 c, t dbSNP:570170198
832 832 c, g dbSNP:746776254
833 833 a, g dbSNP:779676466
836 836 c, t dbSNP:200485062
842 842 c, t dbSNP:138927823
843 843 g, t dbSNP:765361603
860 860 c, g, t dbSNP:146904798
861 861 a, g dbSNP:541222703
875 875 c, g dbSNP:377285325
877 877 a, c dbSNP:776026960
878 878 c, t dbSNP:767718910
883 883 c, t dbSNP:760096011
884 884 a, g dbSNP:573496328
897 897 c, t dbSNP:143947056
912 912 c, t dbSNP:138504221
916 916 c, t dbSNP:776306411
917 917 a, g dbSNP:147061152
929 929 -, a dbSNP:775136193
929 929 a, g dbSNP:746617014
931 931 a, c, g, t dbSNP:745884647
946 946 a, c, g dbSNP:536930894
948 948 a, g dbSNP:575904470
952 952 a, c dbSNP:753934983
953 953 c, t dbSNP:777957953
956 956 c, t dbSNP:147716878
957 957 a, g, t dbSNP:769385447
959 959 a, g dbSNP:267605082
962 962 c, t dbSNP:759810426
969 969 c, g dbSNP:140513686
1010 1010 c, t dbSNP:749969632
1011 1011 a, g dbSNP:537686455
1015 1015 c, t dbSNP:570139600
1030 1030 c, t dbSNP:558462653
1053 1053 a, g dbSNP:186827026
1071 1071 a, g dbSNP:373607901
1081 1081 c, t dbSNP:537972451
1087 1087 c, t dbSNP:568359164
1099 1099 c, t dbSNP:775257114
1104 1104 c, t dbSNP:771588725
1107 1107 c, t dbSNP:759184649
1108 1108 c, g dbSNP:774035693
1115 1115 c, g dbSNP:770790232
1122 1122 c, t dbSNP:749308322
1130 1130 g, t dbSNP:182544870
1132 1132 c, t dbSNP:770050710
1133 1133 a, g dbSNP:568262258
1135 1135 a, g dbSNP:374872971
1139 1139 c, t dbSNP:755179111
1148 1148 a, c dbSNP:747197522
1153 1153 a, g dbSNP:780403434
1157 1157 a, g dbSNP:758756630
1160 1160 a, g dbSNP:151243903
1171 1171 g, t dbSNP:201413321
1177 1177 a, c, g dbSNP:143223388
1183 1183 a, g dbSNP:752176531
1195 1195 c, t dbSNP:149139346
1198 1198 c, t dbSNP:145596938
1209 1209 c, t dbSNP:143526757
1217 1217 a, t dbSNP:766337909
1218 1218 c, t dbSNP:762822596
1223 1223 -, c dbSNP:778700037
1226 1226 a, g, t dbSNP:769651235
1231 1231 c, t dbSNP:748069998
1232 1232 c, t dbSNP:139918604
1233 1233 a, g dbSNP:367883023
1234 1234 a, g dbSNP:747259484
1236 1236 c, t dbSNP:780239925
1238 1238 c, t dbSNP:146494740

Target ORF information:

RefSeq Version XM_005257582
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257582

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGG CACTGAGGCC
CAGAGAGATG ACGTGGCGGT GAGAGGTGTC GGCTCGACAA GGCACCTCCC GCTGCTGATT
GGATTGGAGA AGGGAGCCGC CCAGAGTGGC GTAGGTTCGG CCCTTCCGAG GGCAGCTCCT
GTGTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50586
Accession Version XM_005257583.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1023bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product N-sulphoglucosamine sulphohydrolase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578831247. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGT GACTAGGCTA
CTTTTGCCCA CTGTAAAAGG CACCGTCATC CTGGTATCAA GATTCTGGAG AGGCCAGATT
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_005257640.1
CDS21..1043
Misc Feature(1)78..>995(+)
Misc Feature(2)87..962(+)
Translation
Position Chain Variation Link
4 4 c, t dbSNP:751812573
5 5 c, t dbSNP:566268948
38 38 c, g dbSNP:544042160
39 39 a, g dbSNP:548145029
56 56 c, g dbSNP:777433840
87 87 c, t dbSNP:529571746
96 96 c, t dbSNP:755001766
103 103 -, gct dbSNP:786200990
105 105 a, c dbSNP:562218609
108 108 c, g dbSNP:758793075
109 109 c, t dbSNP:767227727
110 110 a, g dbSNP:549213151
116 116 c, t dbSNP:139850991
117 117 a, g dbSNP:398123246
118 118 a, g dbSNP:762732038
135 135 a, g dbSNP:773137508
136 136 c, t dbSNP:372283734
137 137 a, g dbSNP:201132655
140 140 a, c dbSNP:562933313
141 141 a, g dbSNP:768751214
145 145 -, aca dbSNP:746784710
149 149 c, t dbSNP:747247188
155 155 c, t dbSNP:775770560
156 156 a, g dbSNP:772636496
158 158 c, t dbSNP:746172006
163 163 c, t dbSNP:779581338
164 164 a, g dbSNP:145970971
165 165 c, t dbSNP:749768976
167 167 c, t dbSNP:780614886
168 168 c, g dbSNP:376752886
173 173 c, t dbSNP:372226239
174 174 a, g dbSNP:551094331
175 175 c, t dbSNP:758203457
183 183 c, t dbSNP:368650877
184 184 a, g dbSNP:558991918
186 186 c, t dbSNP:761780038
189 189 a, g dbSNP:753795068
201 201 c, t dbSNP:142309764
202 202 a, g, t dbSNP:573342437
203 203 c, t dbSNP:561475181
209 209 c, g dbSNP:760006618
217 217 c, g, t dbSNP:104894637
218 218 a, g dbSNP:771397375
231 231 c, t dbSNP:398123245
240 240 c, t dbSNP:104894636
241 241 a, g dbSNP:778336949
242 242 c, t dbSNP:768056322
243 243 a, g dbSNP:137953179
255 255 a, c dbSNP:779703983
257 257 c, t dbSNP:758007069
258 258 a, g dbSNP:750048841
261 261 c, t dbSNP:778730409
263 263 a, g, t dbSNP:543297521
270 270 -, catcag dbSNP:757278903
288 288 a, g dbSNP:774010006
296 296 c, g dbSNP:201478799
302 302 c, t dbSNP:748857638
312 312 c, t dbSNP:777669364
317 317 c, g dbSNP:755846091
323 323 c, t dbSNP:150482611
324 324 a, g dbSNP:781207515
328 328 a, g dbSNP:755122392
329 329 a, g dbSNP:751641089
330 330 a, g dbSNP:766542212
333 333 c, t dbSNP:375755239
334 334 a, g dbSNP:375058146
335 335 c, g dbSNP:765693219
343 343 c, t dbSNP:762164487
344 344 a, g dbSNP:147938702
347 347 a, g dbSNP:764531454
350 350 a, g dbSNP:547771707
355 355 c, g dbSNP:773947080
364 364 g, t dbSNP:770321343
367 367 c, g, t dbSNP:148347556
369 369 c, t dbSNP:772732363
370 370 a, g dbSNP:769633955
371 371 c, t dbSNP:747892803
373 373 c, t dbSNP:781299370
384 384 a, g dbSNP:761607612
386 386 a, g dbSNP:776410862
395 395 c, t dbSNP:774482734
396 396 a, c, g dbSNP:775553760
398 398 g, t dbSNP:771878830
399 399 c, g dbSNP:745996666
403 403 c, t dbSNP:104894642
404 404 a, g dbSNP:770944273
410 410 c, g, t dbSNP:111761143
411 411 a, g dbSNP:370636303
417 417 a, c dbSNP:199643693
418 418 c, t dbSNP:781743238
419 419 a, c, g dbSNP:752056541
426 426 a, t dbSNP:764852930
427 427 -, tt dbSNP:754004591
430 430 c, t dbSNP:367805575
431 431 a, g dbSNP:142557761
435 435 a, g, t dbSNP:760564328
436 436 c, t dbSNP:775112689
437 437 a, g dbSNP:142729872
443 443 c, g dbSNP:769601150
451 451 c, t dbSNP:759410190
452 452 c, t dbSNP:200194908
453 453 a, g dbSNP:771068876
457 457 c, t dbSNP:749358773
466 466 g, t dbSNP:773474993
469 469 a, g dbSNP:104894638
475 475 c, t dbSNP:748362261
476 476 c, g dbSNP:781433391
479 479 c, t dbSNP:755311312
481 481 -, t dbSNP:36003850
485 485 g, t dbSNP:747521615
488 488 g, t dbSNP:780700374
489 489 c, g dbSNP:535751912
494 494 c, g, t dbSNP:75828254
495 495 a, g dbSNP:755749619
498 498 c, t dbSNP:189571889
499 499 a, g dbSNP:767224326
500 500 -, t dbSNP:34014912
506 506 c, t dbSNP:371061954
523 523 a, c dbSNP:774333201
525 525 c, t dbSNP:770108057
528 528 c, g dbSNP:527760024
536 536 c, t dbSNP:758123603
539 539 c, t dbSNP:750394348
540 540 c, t dbSNP:765206157
542 542 c, t dbSNP:147064455
543 543 a, g dbSNP:753921560
546 546 a, g dbSNP:141533664
547 547 c, g dbSNP:760996554
548 548 a, c dbSNP:775792252
554 554 c, t dbSNP:139484283
555 555 a, c, g dbSNP:774773010
557 557 c, t dbSNP:771440037
560 560 c, g dbSNP:749721134
562 562 a, g dbSNP:776228545
564 564 c, t dbSNP:529855742
565 565 a, g dbSNP:372911015
576 576 a, t dbSNP:779677047
581 581 g, t dbSNP:758246399
583 583 c, t dbSNP:745651937
587 587 a, g dbSNP:778755182
590 590 c, t dbSNP:149951602
591 591 a, g dbSNP:753666460
594 594 a, g dbSNP:764278674
605 605 c, g dbSNP:756193095
617 617 c, t dbSNP:753030382
618 618 a, g dbSNP:767834681
626 626 c, t dbSNP:544509805
627 627 a, c, g dbSNP:369368791
631 631 c, t dbSNP:766605672
636 636 c, t dbSNP:532180050
637 637 a, c, g dbSNP:104894643
650 650 a, g dbSNP:373031930
652 652 c, t dbSNP:746508510
653 653 a, c dbSNP:187147954
656 656 c, t dbSNP:771680187
657 657 a, c dbSNP:200644359
661 661 a, c dbSNP:74000629
663 663 c, t dbSNP:756939944
665 665 c, t dbSNP:151227799
666 666 a, g dbSNP:777843748
677 677 c, t dbSNP:756315850
678 678 a, c, g, t dbSNP:150508741
681 681 c, t dbSNP:751677255
688 688 c, t dbSNP:760542684
690 690 -, t dbSNP:766980170
695 695 c, g, t dbSNP:34520362
696 696 a, g dbSNP:145967352
700 700 c, g dbSNP:774602372
703 703 a, g dbSNP:771177142
704 704 c, t dbSNP:562717448
707 707 a, c dbSNP:778017231
708 708 c, t dbSNP:756437160
709 709 c, t dbSNP:748671633
710 710 a, g dbSNP:781715169
711 711 -, gcagcccg dbSNP:761216892
717 717 c, g, t dbSNP:374621913
718 718 a, g dbSNP:370291256
721 721 c, g dbSNP:113641837
723 723 a, g dbSNP:753472891
724 724 a, t dbSNP:763800418
730 730 c, t dbSNP:760510972
732 732 a, g dbSNP:752632460
733 733 c, t dbSNP:767416947
740 740 c, t dbSNP:367654527
742 742 c, t dbSNP:774439487
747 747 a, g dbSNP:770944322
749 749 a, c, t dbSNP:17854435
750 750 a, g dbSNP:773480617
754 754 a, g dbSNP:104894635
769 769 c, t dbSNP:765180942
771 771 a, g dbSNP:528633031
772 772 c, g dbSNP:144461610
777 777 -, g dbSNP:760281672
793 793 a, g dbSNP:549606472
797 797 c, t dbSNP:761104676
798 798 a, g dbSNP:776183743
800 800 c, t dbSNP:530964770
801 801 a, g dbSNP:149248428
812 812 c, t dbSNP:147962306
817 817 c, t dbSNP:769121533
818 818 a, g dbSNP:199562202
826 826 a, t dbSNP:780812356
828 828 c, t dbSNP:570170198
832 832 c, g dbSNP:746776254
833 833 a, g dbSNP:779676466
836 836 c, t dbSNP:200485062
842 842 c, t dbSNP:138927823
843 843 g, t dbSNP:765361603
860 860 c, g, t dbSNP:146904798
861 861 a, g dbSNP:541222703
875 875 c, g dbSNP:377285325
877 877 a, c dbSNP:776026960
878 878 c, t dbSNP:767718910
883 883 c, t dbSNP:760096011
884 884 a, g dbSNP:573496328
897 897 c, t dbSNP:143947056
912 912 c, t dbSNP:138504221
916 916 c, t dbSNP:776306411
917 917 a, g dbSNP:147061152
929 929 -, a dbSNP:775136193
929 929 a, g dbSNP:746617014
931 931 a, c, g, t dbSNP:745884647
946 946 a, c, g dbSNP:536930894
948 948 a, g dbSNP:575904470
952 952 a, c dbSNP:753934983
953 953 c, t dbSNP:777957953
956 956 c, t dbSNP:147716878
957 957 a, g, t dbSNP:769385447
959 959 a, g dbSNP:267605082
962 962 c, t dbSNP:759810426
969 969 c, g dbSNP:140513686
998 998 -, a dbSNP:775117492
1004 1004 c, t dbSNP:566562657
1005 1005 a, g dbSNP:76215371
1010 1010 c, t dbSNP:540831886
1027 1027 a, g dbSNP:8078181
1047 1047 c, t dbSNP:759439863
1060 1060 a, g dbSNP:372790630
1062 1062 c, t dbSNP:372586928
1063 1063 a, g dbSNP:146356100
1066 1066 a, g dbSNP:530156168
1071 1071 c, t dbSNP:763985273
1072 1072 a, g dbSNP:150378107
1075 1075 a, g dbSNP:752794881
1082 1082 a, g dbSNP:767438527
1093 1093 c, g dbSNP:759807969
1095 1095 g, t dbSNP:199555156
1097 1097 a, g dbSNP:766816713
1100 1100 c, g dbSNP:201965466
1102 1102 a, g dbSNP:773673818
1108 1108 a, g dbSNP:770449198
1110 1110 c, t dbSNP:748610851
1112 1112 c, t dbSNP:775074934
1113 1113 a, c dbSNP:771482198
1122 1122 c, t dbSNP:375006162
1123 1123 a, g dbSNP:778405709
1128 1128 c, g dbSNP:142664965
1139 1139 a, g dbSNP:749111630

Target ORF information:

RefSeq Version XM_005257583
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257583

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGT GACTAGGCTA
CTTTTGCCCA CTGTAAAAGG CACCGTCATC CTGGTATCAA GATTCTGGAG AGGCCAGATT
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60463
Accession Version XM_011525126.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1011bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product N-sulphoglucosamine sulphohydrolase isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA GGAAAAGAAT
GAGGCTCAGA GAGGTGAAGT AAGCTGCTCA GGATTGCACA GCTGGCACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011523428.1
CDS21..1031
Misc Feature(1)78..>968(+)
Misc Feature(2)87..962(+)
Translation
Position Chain Variation Link
4 4 c, t dbSNP:751812573
5 5 c, t dbSNP:566268948
38 38 c, g dbSNP:544042160
39 39 a, g dbSNP:548145029
56 56 c, g dbSNP:777433840
87 87 c, t dbSNP:529571746
96 96 c, t dbSNP:755001766
103 103 -, gct dbSNP:786200990
105 105 a, c dbSNP:562218609
108 108 c, g dbSNP:758793075
109 109 c, t dbSNP:767227727
110 110 a, g dbSNP:549213151
116 116 c, t dbSNP:139850991
117 117 a, g dbSNP:398123246
118 118 a, g dbSNP:762732038
135 135 a, g dbSNP:773137508
136 136 c, t dbSNP:372283734
137 137 a, g dbSNP:201132655
140 140 a, c dbSNP:562933313
141 141 a, g dbSNP:768751214
145 145 -, aca dbSNP:746784710
149 149 c, t dbSNP:747247188
155 155 c, t dbSNP:775770560
156 156 a, g dbSNP:772636496
158 158 c, t dbSNP:746172006
163 163 c, t dbSNP:779581338
164 164 a, g dbSNP:145970971
165 165 c, t dbSNP:749768976
167 167 c, t dbSNP:780614886
168 168 c, g dbSNP:376752886
173 173 c, t dbSNP:372226239
174 174 a, g dbSNP:551094331
175 175 c, t dbSNP:758203457
183 183 c, t dbSNP:368650877
184 184 a, g dbSNP:558991918
186 186 c, t dbSNP:761780038
189 189 a, g dbSNP:753795068
201 201 c, t dbSNP:142309764
202 202 a, g, t dbSNP:573342437
203 203 c, t dbSNP:561475181
209 209 c, g dbSNP:760006618
217 217 c, g, t dbSNP:104894637
218 218 a, g dbSNP:771397375
231 231 c, t dbSNP:398123245
240 240 c, t dbSNP:104894636
241 241 a, g dbSNP:778336949
242 242 c, t dbSNP:768056322
243 243 a, g dbSNP:137953179
255 255 a, c dbSNP:779703983
257 257 c, t dbSNP:758007069
258 258 a, g dbSNP:750048841
261 261 c, t dbSNP:778730409
263 263 a, g, t dbSNP:543297521
270 270 -, catcag dbSNP:757278903
288 288 a, g dbSNP:774010006
296 296 c, g dbSNP:201478799
302 302 c, t dbSNP:748857638
312 312 c, t dbSNP:777669364
317 317 c, g dbSNP:755846091
323 323 c, t dbSNP:150482611
324 324 a, g dbSNP:781207515
328 328 a, g dbSNP:755122392
329 329 a, g dbSNP:751641089
330 330 a, g dbSNP:766542212
333 333 c, t dbSNP:375755239
334 334 a, g dbSNP:375058146
335 335 c, g dbSNP:765693219
343 343 c, t dbSNP:762164487
344 344 a, g dbSNP:147938702
347 347 a, g dbSNP:764531454
350 350 a, g dbSNP:547771707
355 355 c, g dbSNP:773947080
364 364 g, t dbSNP:770321343
367 367 c, g, t dbSNP:148347556
369 369 c, t dbSNP:772732363
370 370 a, g dbSNP:769633955
371 371 c, t dbSNP:747892803
373 373 c, t dbSNP:781299370
384 384 a, g dbSNP:761607612
386 386 a, g dbSNP:776410862
395 395 c, t dbSNP:774482734
396 396 a, c, g dbSNP:775553760
398 398 g, t dbSNP:771878830
399 399 c, g dbSNP:745996666
403 403 c, t dbSNP:104894642
404 404 a, g dbSNP:770944273
410 410 c, g, t dbSNP:111761143
411 411 a, g dbSNP:370636303
417 417 a, c dbSNP:199643693
418 418 c, t dbSNP:781743238
419 419 a, c, g dbSNP:752056541
426 426 a, t dbSNP:764852930
427 427 -, tt dbSNP:754004591
430 430 c, t dbSNP:367805575
431 431 a, g dbSNP:142557761
435 435 a, g, t dbSNP:760564328
436 436 c, t dbSNP:775112689
437 437 a, g dbSNP:142729872
443 443 c, g dbSNP:769601150
451 451 c, t dbSNP:759410190
452 452 c, t dbSNP:200194908
453 453 a, g dbSNP:771068876
457 457 c, t dbSNP:749358773
466 466 g, t dbSNP:773474993
469 469 a, g dbSNP:104894638
475 475 c, t dbSNP:748362261
476 476 c, g dbSNP:781433391
479 479 c, t dbSNP:755311312
481 481 -, t dbSNP:36003850
485 485 g, t dbSNP:747521615
488 488 g, t dbSNP:780700374
489 489 c, g dbSNP:535751912
494 494 c, g, t dbSNP:75828254
495 495 a, g dbSNP:755749619
498 498 c, t dbSNP:189571889
499 499 a, g dbSNP:767224326
500 500 -, t dbSNP:34014912
506 506 c, t dbSNP:371061954
523 523 a, c dbSNP:774333201
525 525 c, t dbSNP:770108057
528 528 c, g dbSNP:527760024
536 536 c, t dbSNP:758123603
539 539 c, t dbSNP:750394348
540 540 c, t dbSNP:765206157
542 542 c, t dbSNP:147064455
543 543 a, g dbSNP:753921560
546 546 a, g dbSNP:141533664
547 547 c, g dbSNP:760996554
548 548 a, c dbSNP:775792252
554 554 c, t dbSNP:139484283
555 555 a, c, g dbSNP:774773010
557 557 c, t dbSNP:771440037
560 560 c, g dbSNP:749721134
562 562 a, g dbSNP:776228545
564 564 c, t dbSNP:529855742
565 565 a, g dbSNP:372911015
576 576 a, t dbSNP:779677047
581 581 g, t dbSNP:758246399
583 583 c, t dbSNP:745651937
587 587 a, g dbSNP:778755182
590 590 c, t dbSNP:149951602
591 591 a, g dbSNP:753666460
594 594 a, g dbSNP:764278674
605 605 c, g dbSNP:756193095
617 617 c, t dbSNP:753030382
618 618 a, g dbSNP:767834681
626 626 c, t dbSNP:544509805
627 627 a, c, g dbSNP:369368791
631 631 c, t dbSNP:766605672
636 636 c, t dbSNP:532180050
637 637 a, c, g dbSNP:104894643
650 650 a, g dbSNP:373031930
652 652 c, t dbSNP:746508510
653 653 a, c dbSNP:187147954
656 656 c, t dbSNP:771680187
657 657 a, c dbSNP:200644359
661 661 a, c dbSNP:74000629
663 663 c, t dbSNP:756939944
665 665 c, t dbSNP:151227799
666 666 a, g dbSNP:777843748
677 677 c, t dbSNP:756315850
678 678 a, c, g, t dbSNP:150508741
681 681 c, t dbSNP:751677255
688 688 c, t dbSNP:760542684
690 690 -, t dbSNP:766980170
695 695 c, g, t dbSNP:34520362
696 696 a, g dbSNP:145967352
700 700 c, g dbSNP:774602372
703 703 a, g dbSNP:771177142
704 704 c, t dbSNP:562717448
707 707 a, c dbSNP:778017231
708 708 c, t dbSNP:756437160
709 709 c, t dbSNP:748671633
710 710 a, g dbSNP:781715169
711 711 -, gcagcccg dbSNP:761216892
717 717 c, g, t dbSNP:374621913
718 718 a, g dbSNP:370291256
721 721 c, g dbSNP:113641837
723 723 a, g dbSNP:753472891
724 724 a, t dbSNP:763800418
730 730 c, t dbSNP:760510972
732 732 a, g dbSNP:752632460
733 733 c, t dbSNP:767416947
740 740 c, t dbSNP:367654527
742 742 c, t dbSNP:774439487
747 747 a, g dbSNP:770944322
749 749 a, c, t dbSNP:17854435
750 750 a, g dbSNP:773480617
754 754 a, g dbSNP:104894635
769 769 c, t dbSNP:765180942
771 771 a, g dbSNP:528633031
772 772 c, g dbSNP:144461610
777 777 -, g dbSNP:760281672
793 793 a, g dbSNP:549606472
797 797 c, t dbSNP:761104676
798 798 a, g dbSNP:776183743
800 800 c, t dbSNP:530964770
801 801 a, g dbSNP:149248428
812 812 c, t dbSNP:147962306
817 817 c, t dbSNP:769121533
818 818 a, g dbSNP:199562202
826 826 a, t dbSNP:780812356
828 828 c, t dbSNP:570170198
832 832 c, g dbSNP:746776254
833 833 a, g dbSNP:779676466
836 836 c, t dbSNP:200485062
842 842 c, t dbSNP:138927823
843 843 g, t dbSNP:765361603
860 860 c, g, t dbSNP:146904798
861 861 a, g dbSNP:541222703
875 875 c, g dbSNP:377285325
877 877 a, c dbSNP:776026960
878 878 c, t dbSNP:767718910
883 883 c, t dbSNP:760096011
884 884 a, g dbSNP:573496328
897 897 c, t dbSNP:143947056
912 912 c, t dbSNP:138504221
916 916 c, t dbSNP:776306411
917 917 a, g dbSNP:147061152
929 929 -, a dbSNP:775136193
929 929 a, g dbSNP:746617014
931 931 a, c, g, t dbSNP:745884647
946 946 a, c, g dbSNP:536930894
948 948 a, g dbSNP:575904470
952 952 a, c dbSNP:753934983
953 953 c, t dbSNP:777957953
956 956 c, t dbSNP:147716878
957 957 a, g, t dbSNP:769385447
959 959 a, g dbSNP:267605082
962 962 c, t dbSNP:759810426
969 969 c, g dbSNP:140513686
990 990 a, g dbSNP:559844728
996 996 a, g dbSNP:766682820
1013 1013 a, t dbSNP:763466630
1017 1017 c, t dbSNP:541156370

Target ORF information:

RefSeq Version XM_011525126
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011525126

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA GGAAAAGAAT
GAGGCTCAGA GAGGTGAAGT AAGCTGCTCA GGATTGCACA GCTGGCACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60464
Accession Version XM_011525127.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1005bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product N-sulphoglucosamine sulphohydrolase isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA GGAAAAGAAT
GAGGCTCAGA GAGGTGAAGT AAGCTGCTCA GGATTGCACA GCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011523429.1
CDS21..1025
Misc Feature(1)78..>968(+)
Misc Feature(2)87..962(+)
Translation
Position Chain Variation Link
4 4 c, t dbSNP:751812573
5 5 c, t dbSNP:566268948
38 38 c, g dbSNP:544042160
39 39 a, g dbSNP:548145029
56 56 c, g dbSNP:777433840
87 87 c, t dbSNP:529571746
96 96 c, t dbSNP:755001766
103 103 -, gct dbSNP:786200990
105 105 a, c dbSNP:562218609
108 108 c, g dbSNP:758793075
109 109 c, t dbSNP:767227727
110 110 a, g dbSNP:549213151
116 116 c, t dbSNP:139850991
117 117 a, g dbSNP:398123246
118 118 a, g dbSNP:762732038
135 135 a, g dbSNP:773137508
136 136 c, t dbSNP:372283734
137 137 a, g dbSNP:201132655
140 140 a, c dbSNP:562933313
141 141 a, g dbSNP:768751214
145 145 -, aca dbSNP:746784710
149 149 c, t dbSNP:747247188
155 155 c, t dbSNP:775770560
156 156 a, g dbSNP:772636496
158 158 c, t dbSNP:746172006
163 163 c, t dbSNP:779581338
164 164 a, g dbSNP:145970971
165 165 c, t dbSNP:749768976
167 167 c, t dbSNP:780614886
168 168 c, g dbSNP:376752886
173 173 c, t dbSNP:372226239
174 174 a, g dbSNP:551094331
175 175 c, t dbSNP:758203457
183 183 c, t dbSNP:368650877
184 184 a, g dbSNP:558991918
186 186 c, t dbSNP:761780038
189 189 a, g dbSNP:753795068
201 201 c, t dbSNP:142309764
202 202 a, g, t dbSNP:573342437
203 203 c, t dbSNP:561475181
209 209 c, g dbSNP:760006618
217 217 c, g, t dbSNP:104894637
218 218 a, g dbSNP:771397375
231 231 c, t dbSNP:398123245
240 240 c, t dbSNP:104894636
241 241 a, g dbSNP:778336949
242 242 c, t dbSNP:768056322
243 243 a, g dbSNP:137953179
255 255 a, c dbSNP:779703983
257 257 c, t dbSNP:758007069
258 258 a, g dbSNP:750048841
261 261 c, t dbSNP:778730409
263 263 a, g, t dbSNP:543297521
270 270 -, catcag dbSNP:757278903
288 288 a, g dbSNP:774010006
296 296 c, g dbSNP:201478799
302 302 c, t dbSNP:748857638
312 312 c, t dbSNP:777669364
317 317 c, g dbSNP:755846091
323 323 c, t dbSNP:150482611
324 324 a, g dbSNP:781207515
328 328 a, g dbSNP:755122392
329 329 a, g dbSNP:751641089
330 330 a, g dbSNP:766542212
333 333 c, t dbSNP:375755239
334 334 a, g dbSNP:375058146
335 335 c, g dbSNP:765693219
343 343 c, t dbSNP:762164487
344 344 a, g dbSNP:147938702
347 347 a, g dbSNP:764531454
350 350 a, g dbSNP:547771707
355 355 c, g dbSNP:773947080
364 364 g, t dbSNP:770321343
367 367 c, g, t dbSNP:148347556
369 369 c, t dbSNP:772732363
370 370 a, g dbSNP:769633955
371 371 c, t dbSNP:747892803
373 373 c, t dbSNP:781299370
384 384 a, g dbSNP:761607612
386 386 a, g dbSNP:776410862
395 395 c, t dbSNP:774482734
396 396 a, c, g dbSNP:775553760
398 398 g, t dbSNP:771878830
399 399 c, g dbSNP:745996666
403 403 c, t dbSNP:104894642
404 404 a, g dbSNP:770944273
410 410 c, g, t dbSNP:111761143
411 411 a, g dbSNP:370636303
417 417 a, c dbSNP:199643693
418 418 c, t dbSNP:781743238
419 419 a, c, g dbSNP:752056541
426 426 a, t dbSNP:764852930
427 427 -, tt dbSNP:754004591
430 430 c, t dbSNP:367805575
431 431 a, g dbSNP:142557761
435 435 a, g, t dbSNP:760564328
436 436 c, t dbSNP:775112689
437 437 a, g dbSNP:142729872
443 443 c, g dbSNP:769601150
451 451 c, t dbSNP:759410190
452 452 c, t dbSNP:200194908
453 453 a, g dbSNP:771068876
457 457 c, t dbSNP:749358773
466 466 g, t dbSNP:773474993
469 469 a, g dbSNP:104894638
475 475 c, t dbSNP:748362261
476 476 c, g dbSNP:781433391
479 479 c, t dbSNP:755311312
481 481 -, t dbSNP:36003850
485 485 g, t dbSNP:747521615
488 488 g, t dbSNP:780700374
489 489 c, g dbSNP:535751912
494 494 c, g, t dbSNP:75828254
495 495 a, g dbSNP:755749619
498 498 c, t dbSNP:189571889
499 499 a, g dbSNP:767224326
500 500 -, t dbSNP:34014912
506 506 c, t dbSNP:371061954
523 523 a, c dbSNP:774333201
525 525 c, t dbSNP:770108057
528 528 c, g dbSNP:527760024
536 536 c, t dbSNP:758123603
539 539 c, t dbSNP:750394348
540 540 c, t dbSNP:765206157
542 542 c, t dbSNP:147064455
543 543 a, g dbSNP:753921560
546 546 a, g dbSNP:141533664
547 547 c, g dbSNP:760996554
548 548 a, c dbSNP:775792252
554 554 c, t dbSNP:139484283
555 555 a, c, g dbSNP:774773010
557 557 c, t dbSNP:771440037
560 560 c, g dbSNP:749721134
562 562 a, g dbSNP:776228545
564 564 c, t dbSNP:529855742
565 565 a, g dbSNP:372911015
576 576 a, t dbSNP:779677047
581 581 g, t dbSNP:758246399
583 583 c, t dbSNP:745651937
587 587 a, g dbSNP:778755182
590 590 c, t dbSNP:149951602
591 591 a, g dbSNP:753666460
594 594 a, g dbSNP:764278674
605 605 c, g dbSNP:756193095
617 617 c, t dbSNP:753030382
618 618 a, g dbSNP:767834681
626 626 c, t dbSNP:544509805
627 627 a, c, g dbSNP:369368791
631 631 c, t dbSNP:766605672
636 636 c, t dbSNP:532180050
637 637 a, c, g dbSNP:104894643
650 650 a, g dbSNP:373031930
652 652 c, t dbSNP:746508510
653 653 a, c dbSNP:187147954
656 656 c, t dbSNP:771680187
657 657 a, c dbSNP:200644359
661 661 a, c dbSNP:74000629
663 663 c, t dbSNP:756939944
665 665 c, t dbSNP:151227799
666 666 a, g dbSNP:777843748
677 677 c, t dbSNP:756315850
678 678 a, c, g, t dbSNP:150508741
681 681 c, t dbSNP:751677255
688 688 c, t dbSNP:760542684
690 690 -, t dbSNP:766980170
695 695 c, g, t dbSNP:34520362
696 696 a, g dbSNP:145967352
700 700 c, g dbSNP:774602372
703 703 a, g dbSNP:771177142
704 704 c, t dbSNP:562717448
707 707 a, c dbSNP:778017231
708 708 c, t dbSNP:756437160
709 709 c, t dbSNP:748671633
710 710 a, g dbSNP:781715169
711 711 -, gcagcccg dbSNP:761216892
717 717 c, g, t dbSNP:374621913
718 718 a, g dbSNP:370291256
721 721 c, g dbSNP:113641837
723 723 a, g dbSNP:753472891
724 724 a, t dbSNP:763800418
730 730 c, t dbSNP:760510972
732 732 a, g dbSNP:752632460
733 733 c, t dbSNP:767416947
740 740 c, t dbSNP:367654527
742 742 c, t dbSNP:774439487
747 747 a, g dbSNP:770944322
749 749 a, c, t dbSNP:17854435
750 750 a, g dbSNP:773480617
754 754 a, g dbSNP:104894635
769 769 c, t dbSNP:765180942
771 771 a, g dbSNP:528633031
772 772 c, g dbSNP:144461610
777 777 -, g dbSNP:760281672
793 793 a, g dbSNP:549606472
797 797 c, t dbSNP:761104676
798 798 a, g dbSNP:776183743
800 800 c, t dbSNP:530964770
801 801 a, g dbSNP:149248428
812 812 c, t dbSNP:147962306
817 817 c, t dbSNP:769121533
818 818 a, g dbSNP:199562202
826 826 a, t dbSNP:780812356
828 828 c, t dbSNP:570170198
832 832 c, g dbSNP:746776254
833 833 a, g dbSNP:779676466
836 836 c, t dbSNP:200485062
842 842 c, t dbSNP:138927823
843 843 g, t dbSNP:765361603
860 860 c, g, t dbSNP:146904798
861 861 a, g dbSNP:541222703
875 875 c, g dbSNP:377285325
877 877 a, c dbSNP:776026960
878 878 c, t dbSNP:767718910
883 883 c, t dbSNP:760096011
884 884 a, g dbSNP:573496328
897 897 c, t dbSNP:143947056
912 912 c, t dbSNP:138504221
916 916 c, t dbSNP:776306411
917 917 a, g dbSNP:147061152
929 929 -, a dbSNP:775136193
929 929 a, g dbSNP:746617014
931 931 a, c, g, t dbSNP:745884647
946 946 a, c, g dbSNP:536930894
948 948 a, g dbSNP:575904470
952 952 a, c dbSNP:753934983
953 953 c, t dbSNP:777957953
956 956 c, t dbSNP:147716878
957 957 a, g, t dbSNP:769385447
959 959 a, g dbSNP:267605082
962 962 c, t dbSNP:759810426
969 969 c, g dbSNP:140513686
990 990 a, g dbSNP:559844728
996 996 a, g dbSNP:766682820
1013 1013 a, t dbSNP:763466630
1017 1017 c, t dbSNP:541156370
1027 1027 a, c dbSNP:747197522
1032 1032 a, g dbSNP:780403434
1036 1036 a, g dbSNP:758756630
1039 1039 a, g dbSNP:151243903
1050 1050 g, t dbSNP:201413321
1056 1056 a, c, g dbSNP:143223388
1062 1062 a, g dbSNP:752176531
1074 1074 c, t dbSNP:149139346
1077 1077 c, t dbSNP:145596938
1088 1088 c, t dbSNP:143526757
1096 1096 a, t dbSNP:766337909
1097 1097 c, t dbSNP:762822596
1102 1102 -, c dbSNP:778700037
1105 1105 a, g, t dbSNP:769651235
1110 1110 c, t dbSNP:748069998
1111 1111 c, t dbSNP:139918604
1112 1112 a, g dbSNP:367883023

Target ORF information:

RefSeq Version XM_011525127
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011525127

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA GGAAAAGAAT
GAGGCTCAGA GAGGTGAAGT AAGCTGCTCA GGATTGCACA GCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18039
Accession Version NM_000199.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1509bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product N-sulphoglucosamine sulphohydrolase precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC319407.1, U30894.1, AC087741.18, AW274014.1 and BF445930.1. This sequence is a reference standard in the RefSeqGene project. On Apr 12, 2008 this sequence version replaced gi:31543619. Summary: This gene encodes one of several enzymes involved in the lysosomal degradation of heparan sulfate. Mutations in this gene are associated with Sanfilippo syndrome A, one type of the lysosomal storage disease mucopolysaccaridosis III, which results from impaired degradation of heparan sulfate. Transcripts of varying sizes have been reported but their biological validity has not been determined. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC047318.1, U30894.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA CCTCACGCCC
ACCATCTTGG ATTGGTTCTC GATCCCGTAC CCCAGCTACG CCATCTTTGG CTCGAAGACC
ATCCACCTCA CTGGCCGGTC CCTCCTGCCG GCGCTGGAGG CCGAGCCCCT CTGGGCCACC
GTCTTTGGCA GCCAGAGCCA CCACGAGGTC ACCATGTCCT ACCCCATGCG CTCCGTGCAG
CACCGGCACT TCCGCCTCGT GCACAACCTC AACTTCAAGA TGCCCTTTCC CATCGACCAG
GACTTCTACG TCTCACCCAC CTTCCAGGAC CTCCTGAACC GCACCACAGC TGGTCAGCCC
ACGGGCTGGT ACAAGGACCT CCGTCATTAC TACTACCGGG CGCGCTGGGA GCTCTACGAC
CGGAGCCGGG ACCCCCACGA GACCCAGAAC CTGGCCACCG ACCCGCGCTT TGCTCAGCTT
CTGGAGATGC TTCGGGACCA GCTGGCCAAG TGGCAGTGGG AGACCCACGA CCCCTGGGTG
TGCGCCCCCG ACGGCGTCCT GGAGGAGAAG CTCTCTCCCC AGTGCCAGCC CCTCCACAAT
GAGCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_000190.1
CDS88..1596
Misc Feature(1)145..1419(+)
Misc Feature(2)154..1071(+)
Misc Feature(3)877..879(+)
Exon (1)1..175
Gene:SGSH
Gene Synonym:
Exon (2)176..336
Gene:SGSH
Gene Synonym:
Exon (3)337..442
Gene:SGSH
Gene Synonym:
Exon (4)443..593
Gene:SGSH
Gene Synonym:
Exon (5)594..750
Gene:SGSH
Gene Synonym:
Exon (6)751..832
Gene:SGSH
Gene Synonym:
Exon (7)833..1036
Gene:SGSH
Gene Synonym:
Exon (8)1037..2768
Gene:SGSH
Gene Synonym:
Translation
Position Chain Variation Link
26 26 c, g dbSNP:71389741
55 55 a, g dbSNP:748426231
59 59 a, c dbSNP:781265421
60 60 -, ggatcc dbSNP:769131507
60 60 c, t dbSNP:552260516
65 65 a, c dbSNP:533566888
71 71 c, t dbSNP:751812573
72 72 c, t dbSNP:566268948
105 105 c, g dbSNP:544042160
106 106 a, g dbSNP:548145029
123 123 c, g dbSNP:777433840
154 154 c, t dbSNP:529571746
163 163 c, t dbSNP:755001766
170 170 -, gct dbSNP:786200990
172 172 a, c dbSNP:562218609
175 175 c, g dbSNP:758793075
176 176 c, t dbSNP:767227727
177 177 a, g dbSNP:549213151
183 183 c, t dbSNP:139850991
184 184 a, g dbSNP:398123246
185 185 a, g dbSNP:762732038
202 202 a, g dbSNP:773137508
203 203 c, t dbSNP:372283734
204 204 a, g dbSNP:201132655
207 207 a, c dbSNP:562933313
208 208 a, g dbSNP:768751214
212 212 -, aca dbSNP:746784710
216 216 c, t dbSNP:747247188
222 222 c, t dbSNP:775770560
223 223 a, g dbSNP:772636496
225 225 c, t dbSNP:746172006
230 230 c, t dbSNP:779581338
231 231 a, g dbSNP:145970971
232 232 c, t dbSNP:749768976
234 234 c, t dbSNP:780614886
235 235 c, g dbSNP:376752886
240 240 c, t dbSNP:372226239
241 241 a, g dbSNP:551094331
242 242 c, t dbSNP:758203457
250 250 c, t dbSNP:368650877
251 251 a, g dbSNP:558991918
253 253 c, t dbSNP:761780038
256 256 a, g dbSNP:753795068
268 268 c, t dbSNP:142309764
269 269 a, g, t dbSNP:573342437
270 270 c, t dbSNP:561475181
276 276 c, g dbSNP:760006618
284 284 c, g, t dbSNP:104894637
285 285 a, g dbSNP:771397375
298 298 c, t dbSNP:398123245
307 307 c, t dbSNP:104894636
308 308 a, g dbSNP:778336949
309 309 c, t dbSNP:768056322
310 310 a, g dbSNP:137953179
322 322 a, c dbSNP:779703983
324 324 c, t dbSNP:758007069
325 325 a, g dbSNP:750048841
328 328 c, t dbSNP:778730409
330 330 a, g, t dbSNP:543297521
337 337 -, catcag dbSNP:757278903
355 355 a, g dbSNP:774010006
363 363 c, g dbSNP:201478799
369 369 c, t dbSNP:748857638
379 379 c, t dbSNP:777669364
384 384 c, g dbSNP:755846091
390 390 c, t dbSNP:150482611
391 391 a, g dbSNP:781207515
395 395 a, g dbSNP:755122392
396 396 a, g dbSNP:751641089
397 397 a, g dbSNP:766542212
400 400 c, t dbSNP:375755239
401 401 a, g dbSNP:375058146
402 402 c, g dbSNP:765693219
410 410 c, t dbSNP:762164487
411 411 a, g dbSNP:147938702
414 414 a, g dbSNP:764531454
417 417 a, g dbSNP:547771707
422 422 c, g dbSNP:773947080
431 431 g, t dbSNP:770321343
434 434 c, g, t dbSNP:148347556
436 436 c, t dbSNP:772732363
437 437 a, g dbSNP:769633955
438 438 c, t dbSNP:747892803
440 440 c, t dbSNP:781299370
451 451 a, g dbSNP:761607612
453 453 a, g dbSNP:776410862
462 462 c, t dbSNP:774482734
463 463 a, c, g dbSNP:775553760
465 465 g, t dbSNP:771878830
466 466 c, g dbSNP:745996666
470 470 c, t dbSNP:104894642
471 471 a, g dbSNP:770944273
477 477 c, g, t dbSNP:111761143
478 478 a, g dbSNP:370636303
484 484 a, c dbSNP:199643693
485 485 c, t dbSNP:781743238
486 486 a, c, g dbSNP:752056541
493 493 a, t dbSNP:764852930
494 494 -, tt dbSNP:754004591
497 497 c, t dbSNP:367805575
498 498 a, g dbSNP:142557761
502 502 a, g, t dbSNP:760564328
503 503 c, t dbSNP:775112689
504 504 a, g dbSNP:142729872
510 510 c, g dbSNP:769601150
518 518 c, t dbSNP:759410190
519 519 c, t dbSNP:200194908
520 520 a, g dbSNP:771068876
524 524 c, t dbSNP:749358773
533 533 g, t dbSNP:773474993
536 536 a, g dbSNP:104894638
542 542 c, t dbSNP:748362261
543 543 c, g dbSNP:781433391
546 546 c, t dbSNP:755311312
548 548 -, t dbSNP:36003850
552 552 g, t dbSNP:747521615
555 555 g, t dbSNP:780700374
556 556 c, g dbSNP:535751912
561 561 c, g, t dbSNP:75828254
562 562 a, g dbSNP:755749619
565 565 c, t dbSNP:189571889
566 566 a, g dbSNP:767224326
567 567 -, t dbSNP:34014912
573 573 c, t dbSNP:371061954
590 590 a, c dbSNP:774333201
592 592 c, t dbSNP:770108057
595 595 c, g dbSNP:527760024
603 603 c, t dbSNP:758123603
606 606 c, t dbSNP:750394348
607 607 c, t dbSNP:765206157
609 609 c, t dbSNP:147064455
610 610 a, g dbSNP:753921560
613 613 a, g dbSNP:141533664
614 614 c, g dbSNP:760996554
615 615 a, c dbSNP:775792252
621 621 c, t dbSNP:139484283
622 622 a, c, g dbSNP:774773010
624 624 c, t dbSNP:771440037
627 627 c, g dbSNP:749721134
629 629 a, g dbSNP:776228545
631 631 c, t dbSNP:529855742
632 632 a, g dbSNP:372911015
643 643 a, t dbSNP:779677047
648 648 g, t dbSNP:758246399
650 650 c, t dbSNP:745651937
654 654 a, g dbSNP:778755182
657 657 c, t dbSNP:149951602
658 658 a, g dbSNP:753666460
661 661 a, g dbSNP:764278674
672 672 c, g dbSNP:756193095
684 684 c, t dbSNP:753030382
685 685 a, g dbSNP:767834681
693 693 c, t dbSNP:544509805
694 694 a, c, g dbSNP:369368791
698 698 c, t dbSNP:766605672
703 703 c, t dbSNP:532180050
704 704 a, c, g dbSNP:104894643
717 717 a, g dbSNP:373031930
719 719 c, t dbSNP:746508510
720 720 a, c dbSNP:187147954
723 723 c, t dbSNP:771680187
724 724 a, c dbSNP:200644359
728 728 a, c dbSNP:74000629
730 730 c, t dbSNP:756939944
732 732 c, t dbSNP:151227799
733 733 a, g dbSNP:777843748
744 744 c, t dbSNP:756315850
745 745 a, c, g, t dbSNP:150508741
748 748 c, t dbSNP:751677255
755 755 c, t dbSNP:760542684
757 757 -, t dbSNP:766980170
762 762 c, g, t dbSNP:34520362
763 763 a, g dbSNP:145967352
767 767 c, g dbSNP:774602372
770 770 a, g dbSNP:771177142
771 771 c, t dbSNP:562717448
774 774 a, c dbSNP:778017231
775 775 c, t dbSNP:756437160
776 776 c, t dbSNP:748671633
777 777 a, g dbSNP:781715169
778 778 -, gcagcccg dbSNP:761216892
784 784 c, g, t dbSNP:374621913
785 785 a, g dbSNP:370291256
788 788 c, g dbSNP:113641837
790 790 a, g dbSNP:753472891
791 791 a, t dbSNP:763800418
797 797 c, t dbSNP:760510972
799 799 a, g dbSNP:752632460
800 800 c, t dbSNP:767416947
807 807 c, t dbSNP:367654527
809 809 c, t dbSNP:774439487
814 814 a, g dbSNP:770944322
816 816 a, c, t dbSNP:17854435
817 817 a, g dbSNP:773480617
821 821 a, g dbSNP:104894635
836 836 c, t dbSNP:765180942
838 838 a, g dbSNP:528633031
839 839 c, g dbSNP:144461610
844 844 -, g dbSNP:760281672
860 860 a, g dbSNP:549606472
864 864 c, t dbSNP:761104676
865 865 a, g dbSNP:776183743
867 867 c, t dbSNP:530964770
868 868 a, g dbSNP:149248428
879 879 c, t dbSNP:147962306
884 884 c, t dbSNP:769121533
885 885 a, g dbSNP:199562202
893 893 a, t dbSNP:780812356
895 895 c, t dbSNP:570170198
899 899 c, g dbSNP:746776254
900 900 a, g dbSNP:779676466
903 903 c, t dbSNP:200485062
909 909 c, t dbSNP:138927823
910 910 g, t dbSNP:765361603
927 927 c, g, t dbSNP:146904798
928 928 a, g dbSNP:541222703
942 942 c, g dbSNP:377285325
944 944 a, c dbSNP:776026960
945 945 c, t dbSNP:767718910
950 950 c, t dbSNP:760096011
951 951 a, g dbSNP:573496328
964 964 c, t dbSNP:143947056
979 979 c, t dbSNP:138504221
983 983 c, t dbSNP:776306411
984 984 a, g dbSNP:147061152
996 996 -, a dbSNP:775136193
996 996 a, g dbSNP:746617014
998 998 a, c, g, t dbSNP:745884647
1013 1013 a, c, g dbSNP:536930894
1015 1015 a, g dbSNP:575904470
1019 1019 a, c dbSNP:753934983
1020 1020 c, t dbSNP:777957953
1023 1023 c, t dbSNP:147716878
1024 1024 a, g, t dbSNP:769385447
1026 1026 a, g dbSNP:267605082
1029 1029 c, t dbSNP:759810426
1036 1036 c, g dbSNP:140513686
1039 1039 a, c dbSNP:747197522
1044 1044 a, g dbSNP:780403434
1048 1048 a, g dbSNP:758756630
1051 1051 a, g dbSNP:151243903
1062 1062 g, t dbSNP:201413321
1068 1068 a, c, g dbSNP:143223388
1074 1074 a, g dbSNP:752176531
1086 1086 c, t dbSNP:149139346
1089 1089 c, t dbSNP:145596938
1100 1100 c, t dbSNP:143526757
1108 1108 a, t dbSNP:766337909
1109 1109 c, t dbSNP:762822596
1114 1114 -, c dbSNP:778700037
1117 1117 a, g, t dbSNP:769651235
1122 1122 c, t dbSNP:748069998
1123 1123 c, t dbSNP:139918604
1124 1124 a, g dbSNP:367883023
1125 1125 a, g dbSNP:747259484
1127 1127 c, t dbSNP:780239925
1129 1129 c, t dbSNP:146494740
1136 1136 c, t dbSNP:144035603
1137 1137 a, g dbSNP:373992449
1139 1139 c, t dbSNP:139075692
1140 1140 a, g dbSNP:757723989
1149 1149 c, t dbSNP:150750383
1150 1150 a, g dbSNP:766938111
1154 1154 a, c dbSNP:754499454
1156 1156 c, t dbSNP:71389740
1163 1163 c, t dbSNP:202150579
1167 1167 -, c dbSNP:770947426
1167 1167 c, t dbSNP:766102096
1168 1168 a, g dbSNP:9894254
1176 1176 c, t dbSNP:367631292
1191 1191 c, t dbSNP:111800668
1192 1192 a, c, g dbSNP:104894640
1195 1195 a, g, t dbSNP:201061563
1203 1203 a, g dbSNP:58786455
1205 1205 a, c dbSNP:768673952
1209 1209 a, c dbSNP:760719845
1214 1214 c, t dbSNP:775729169
1216 1216 c, t dbSNP:772311757
1217 1217 a, g dbSNP:746037899
1218 1218 c, t dbSNP:779260016
1221 1221 c, t dbSNP:771257931
1222 1222 -, g dbSNP:749292976
1222 1222 a, g dbSNP:541847913
1222 1222 -, g dbSNP:777956287
1226 1226 a, g dbSNP:144143780
1230 1230 a, c dbSNP:754489648
1231 1231 -, agcgcc dbSNP:398123244
1231 1231 c, t dbSNP:751150121
1232 1232 a, g dbSNP:779661554
1240 1240 c, g, t dbSNP:374599600
1245 1245 c, t dbSNP:765011477
1246 1246 a, g dbSNP:62620232
1249 1249 c, t dbSNP:753766233
1254 1254 a, c dbSNP:764057581
1269 1269 g, t dbSNP:34297805
1273 1273 c, t dbSNP:775569624
1277 1277 c, t dbSNP:772080776
1281 1281 c, t dbSNP:759758209
1284 1284 c, t dbSNP:371397297
1288 1288 a, g dbSNP:771251355
1296 1296 c, t dbSNP:115750405
1297 1297 a, g dbSNP:191465934
1299 1299 c, t dbSNP:558089661
1305 1305 c, t dbSNP:770446973
1316 1316 a, g dbSNP:748612821
1323 1323 a, g dbSNP:779718665
1328 1328 a, g, t dbSNP:200320139
1331 1331 c, g dbSNP:778602791
1334 1334 c, t dbSNP:757039773
1337 1337 c, g dbSNP:753694088
1339 1339 c, g dbSNP:763743161
1340 1340 a, g dbSNP:760618916
1346 1346 c, t dbSNP:150222010
1349 1349 c, t dbSNP:767649680
1350 1350 a, c, g dbSNP:140931352
1357 1357 c, t dbSNP:766657191
1360 1360 a, g dbSNP:763038283
1369 1369 c, t dbSNP:138267034
1370 1370 a, g dbSNP:144862290
1373 1373 a, g dbSNP:770220285
1383 1383 c, t dbSNP:141153056
1384 1384 c, t dbSNP:777267343
1385 1385 a, g dbSNP:104894641
1387 1387 c, g, t dbSNP:778242416
1388 1388 c, t dbSNP:76375023
1389 1389 a, g dbSNP:376787615
1390 1390 c, t dbSNP:373496399
1391 1391 a, g dbSNP:370528435
1399 1399 c, t dbSNP:752686885
1404 1404 c, t dbSNP:146522699
1407 1407 a, c, t dbSNP:751683275
1408 1408 c, t dbSNP:751604358
1409 1409 a, g dbSNP:142599919
1410 1410 c, g dbSNP:202209795
1414 1414 c, t dbSNP:765758095
1415 1415 a, g dbSNP:377605690
1417 1417 a, g, t dbSNP:373624543
1419 1419 c, t dbSNP:767871704
1424 1424 a, g dbSNP:148876719
1425 1425 c, g, t dbSNP:762208419
1426 1426 a, g dbSNP:104894639
1432 1432 c, t dbSNP:755951235
1438 1438 c, g dbSNP:747970238
1445 1445 c, g dbSNP:780918283
1446 1446 c, t dbSNP:751980056
1447 1447 a, g dbSNP:552049399
1448 1448 a, t dbSNP:145490072
1451 1451 c, t dbSNP:766272590
1452 1452 a, g dbSNP:750709138
1454 1454 a, g dbSNP:7503034
1455 1455 a, c dbSNP:762083993
1480 1480 c, t dbSNP:754273400
1482 1482 a, g dbSNP:764491308
1491 1491 a, g dbSNP:368952369
1512 1512 a, c dbSNP:775855154
1515 1515 c, t dbSNP:139460639
1516 1516 -, g dbSNP:781572815
1521 1521 c, g dbSNP:762345260
1524 1524 g, t dbSNP:772670559
1527 1527 a, g dbSNP:769459888
1530 1530 c, t dbSNP:747742566
1531 1531 a, g dbSNP:780969693
1532 1532 c, t dbSNP:376296818
1533 1533 c, t dbSNP:113679696
1536 1536 c, t dbSNP:779836312
1537 1537 a, g dbSNP:190505648
1539 1539 c, t dbSNP:763360021
1540 1540 a, g dbSNP:779083605
1542 1542 c, t dbSNP:757563981
1543 1543 a, c, g dbSNP:764585957
1549 1549 a, g dbSNP:145645179
1551 1551 c, g dbSNP:753295288
1552 1552 a, g dbSNP:767878626
1559 1559 c, g, t dbSNP:529747766
1563 1563 c, t dbSNP:769228670
1568 1568 a, g dbSNP:562445330
1574 1574 a, t dbSNP:776255667
1581 1581 c, t dbSNP:768548479
1585 1585 a, c dbSNP:746752625
1586 1586 a, g dbSNP:779856036
1587 1587 c, t dbSNP:771923742
1599 1599 a, g dbSNP:745738178
1609 1609 g, t dbSNP:779134651
1616 1616 c, t dbSNP:757408208
1618 1618 c, t dbSNP:754171086
1620 1620 c, t dbSNP:777834421
1621 1621 a, g dbSNP:756541046
1625 1625 c, g dbSNP:753027133
1638 1638 -, ga dbSNP:755392605
1641 1641 a, c dbSNP:767867805
1652 1652 a, g dbSNP:544198851
1665 1665 c, t dbSNP:576879796
1680 1680 a, g dbSNP:776117969
1689 1689 a, g dbSNP:111628992
1703 1703 c, t dbSNP:567331483
1722 1722 a, g dbSNP:546128310
1741 1741 -, gg dbSNP:561581731
1746 1746 c, t dbSNP:572591798
1762 1762 a, g dbSNP:770317133
1794 1794 c, t dbSNP:554079984
1797 1797 a, t dbSNP:139066649
1807 1807 c, t dbSNP:373690501
1808 1808 c, t dbSNP:574766725
1823 1823 c, t dbSNP:771727008
1828 1828 c, t dbSNP:113462619
1830 1830 g, t dbSNP:773111649
1838 1838 c, t dbSNP:538196345
1841 1841 a, g dbSNP:186034906
1873 1873 c, t dbSNP:115999892
1874 1874 a, g dbSNP:533785180
1900 1900 a, g dbSNP:546639018
1905 1905 a, g dbSNP:566654466
1911 1911 c, t dbSNP:2071148
1912 1912 a, g dbSNP:112147015
1974 1974 a, g dbSNP:372712517
2011 2011 a, g dbSNP:141810727
2013 2013 c, t dbSNP:749148905
2053 2053 c, t dbSNP:780068466
2054 2054 a, g dbSNP:373019387
2055 2055 c, t dbSNP:532130517
2067 2067 a, g dbSNP:138717045
2097 2097 c, t dbSNP:377165572
2101 2101 c, g dbSNP:781437136
2118 2118 a, g dbSNP:572581302
2143 2143 c, t dbSNP:768987400
2166 2166 g, t dbSNP:560338880
2234 2234 c, t dbSNP:557625052
2253 2253 c, t dbSNP:564023302
2265 2265 c, t dbSNP:747392113
2273 2273 a, g dbSNP:781501414
2280 2280 c, t dbSNP:757551760
2286 2286 g, t dbSNP:544420951
2297 2297 c, t dbSNP:764534420
2321 2321 c, t dbSNP:574803653
2322 2322 a, g dbSNP:575555131
2346 2346 c, t dbSNP:182008915
2347 2347 a, g dbSNP:753104840
2348 2348 a, g dbSNP:544324749
2359 2359 a, g dbSNP:111957961
2367 2367 c, g dbSNP:765797570
2379 2379 a, g dbSNP:577158386
2394 2394 c, t dbSNP:111693111
2429 2429 a, g dbSNP:2012452
2436 2436 a, g dbSNP:709678
2444 2444 c, g dbSNP:709677
2450 2450 a, g dbSNP:554819186
2451 2451 c, t dbSNP:1046832
2460 2460 c, t dbSNP:190998203
2473 2473 a, g dbSNP:530823641
2477 2477 c, g dbSNP:186120448
2486 2486 a, g dbSNP:111726823
2493 2493 a, g dbSNP:561947366
2508 2508 c, t dbSNP:200388157
2524 2524 a, g dbSNP:147726784
2530 2530 a, g dbSNP:774032554
2533 2533 a, g dbSNP:546252504
2540 2540 g, t dbSNP:202042823
2549 2549 a, g dbSNP:150031854
2631 2631 a, g dbSNP:560611957
2633 2633 -, g dbSNP:755197369
2633 2633 a, g dbSNP:542018784
2718 2718 a, g dbSNP:530225549
2727 2727 a, g dbSNP:541645056
2749 2749 a, g dbSNP:544872567
2761 2761 c, t dbSNP:572959943

Target ORF information:

RefSeq Version NM_000199
Organism Homo sapiens (human)
Definition Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000199

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA CCTCACGCCC
ACCATCTTGG ATTGGTTCTC GATCCCGTAC CCCAGCTACG CCATCTTTGG CTCGAAGACC
ATCCACCTCA CTGGCCGGTC CCTCCTGCCG GCGCTGGAGG CCGAGCCCCT CTGGGCCACC
GTCTTTGGCA GCCAGAGCCA CCACGAGGTC ACCATGTCCT ACCCCATGCG CTCCGTGCAG
CACCGGCACT TCCGCCTCGT GCACAACCTC AACTTCAAGA TGCCCTTTCC CATCGACCAG
GACTTCTACG TCTCACCCAC CTTCCAGGAC CTCCTGAACC GCACCACAGC TGGTCAGCCC
ACGGGCTGGT ACAAGGACCT CCGTCATTAC TACTACCGGG CGCGCTGGGA GCTCTACGAC
CGGAGCCGGG ACCCCCACGA GACCCAGAAC CTGGCCACCG ACCCGCGCTT TGCTCAGCTT
CTGGAGATGC TTCGGGACCA GCTGGCCAAG TGGCAGTGGG AGACCCACGA CCCCTGGGTG
TGCGCCCCCG ACGGCGTCCT GGAGGAGAAG CTCTCTCCCC AGTGCCAGCC CCTCCACAAT
GAGCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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