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SGSH cDNA ORF clone, Homo sapiens (human)

Gene Symbol SGSH
Entrez Gene ID 6448
Full Name N-sulfoglucosamine sulfohydrolase
Synonyms HSS, MPS3A, SFMD
General protein information
Preferred Names
N-sulphoglucosamine sulphohydrolase
Names
N-sulphoglucosamine sulphohydrolase
sulphamidase
heparan sulfate sulfatase
sulfoglucosamine sulfamidase
mucopolysaccharidosis type IIIA
NP_000190.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q25.3

Summary This gene encodes one of several enzymes involved in the lysosomal degradation of heparan sulfate. Mutations in this gene are associated with Sanfilippo syndrome A, one type of the lysosomal storage disease mucopolysaccaridosis III, which results from impaired degradation of heparan sulfate. Transcripts of varying sizes have been reported but their biological validity has not been determined. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

605270

Disorder Html: Mucopolysaccharidisis type IIIA (Sanfilippo A), 252900 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_005257582 XP_005257639 N-sulphoglucosamine sulphohydrolase isoform X1
XM_005257583 XP_005257640 N-sulphoglucosamine sulphohydrolase isoform X2
XM_011525126 XP_011523428 N-sulphoglucosamine sulphohydrolase isoform X3
XM_011525127 XP_011523429 N-sulphoglucosamine sulphohydrolase isoform X4
NM_000199 NP_000190 N-sulphoglucosamine sulphohydrolase precursor



Homo sapiens (human) SGSH NP_000190.1
Macaca mulatta (Rhesus monkey) SGSH XP_001110101.2
Canis lupus familiaris (dog) SGSH NP_001003114.1
Bos taurus (cattle) SGSH NP_001095659.2
Mus musculus (house mouse) Sgsh NP_061292.2
Gallus gallus (chicken) SGSH XP_420084.4
Danio rerio (zebrafish) sgsh NP_001116740.1
Drosophila melanogaster (fruit fly) CG14291 NP_650760.1
Xenopus (Silurana) tropicalis (western clawed frog) sgsh NP_001120065.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SGSH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SGSH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu50585 XM_005257582 PREDICTED: Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu50586 XM_005257583 PREDICTED: Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu60463 XM_011525126 PREDICTED: Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu60464 XM_011525127 PREDICTED: Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu18039
NM_000199 Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu50585
Clone ID Related Accession (Same CDS sequence) XM_005257582
Accession Version XM_005257582.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1089bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product N-sulphoglucosamine sulphohydrolase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530412677. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGG CACTGAGGCC
CAGAGAGATG ACGTGGCGGT GAGAGGTGTC GGCTCGACAA GGCACCTCCC GCTGCTGATT
GGATTGGAGA AGGGAGCCGC CCAGAGTGGC GTAGGTTCGG CCCTTCCGAG GGCAGCTCCT
GTGTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005257639.1
CDS21..1109
Misc Feature(1)78..>968(+)
Misc Feature(2)87..962(+)
Translation

Target ORF information:

RefSeq Version XM_005257582
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257582

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGG CACTGAGGCC
CAGAGAGATG ACGTGGCGGT GAGAGGTGTC GGCTCGACAA GGCACCTCCC GCTGCTGATT
GGATTGGAGA AGGGAGCCGC CCAGAGTGGC GTAGGTTCGG CCCTTCCGAG GGCAGCTCCT
GTGTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50586
Clone ID Related Accession (Same CDS sequence) XM_005257583
Accession Version XM_005257583.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1023bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product N-sulphoglucosamine sulphohydrolase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578831247. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGT GACTAGGCTA
CTTTTGCCCA CTGTAAAAGG CACCGTCATC CTGGTATCAA GATTCTGGAG AGGCCAGATT
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005257640.1
CDS21..1043
Misc Feature(1)78..>995(+)
Misc Feature(2)87..962(+)
Translation

Target ORF information:

RefSeq Version XM_005257583
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257583

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGT GACTAGGCTA
CTTTTGCCCA CTGTAAAAGG CACCGTCATC CTGGTATCAA GATTCTGGAG AGGCCAGATT
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60463
Clone ID Related Accession (Same CDS sequence) XM_011525126
Accession Version XM_011525126.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1011bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product N-sulphoglucosamine sulphohydrolase isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA GGAAAAGAAT
GAGGCTCAGA GAGGTGAAGT AAGCTGCTCA GGATTGCACA GCTGGCACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523428.1
CDS21..1031
Misc Feature(1)78..>968(+)
Misc Feature(2)87..962(+)
Translation

Target ORF information:

RefSeq Version XM_011525126
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011525126

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA GGAAAAGAAT
GAGGCTCAGA GAGGTGAAGT AAGCTGCTCA GGATTGCACA GCTGGCACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60464
Clone ID Related Accession (Same CDS sequence) XM_011525127
Accession Version XM_011525127.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1005bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product N-sulphoglucosamine sulphohydrolase isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA GGAAAAGAAT
GAGGCTCAGA GAGGTGAAGT AAGCTGCTCA GGATTGCACA GCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523429.1
CDS21..1025
Misc Feature(1)78..>968(+)
Misc Feature(2)87..962(+)
Translation

Target ORF information:

RefSeq Version XM_011525127
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011525127

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA GGAAAAGAAT
GAGGCTCAGA GAGGTGAAGT AAGCTGCTCA GGATTGCACA GCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18039
Clone ID Related Accession (Same CDS sequence) NM_000199
Accession Version NM_000199.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1509bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product N-sulphoglucosamine sulphohydrolase precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC319407.1, U30894.1, AC087741.18, AW274014.1 and BF445930.1. This sequence is a reference standard in the RefSeqGene project. On Apr 12, 2008 this sequence version replaced gi:31543619. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC047318.1, U30894.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA CCTCACGCCC
ACCATCTTGG ATTGGTTCTC GATCCCGTAC CCCAGCTACG CCATCTTTGG CTCGAAGACC
ATCCACCTCA CTGGCCGGTC CCTCCTGCCG GCGCTGGAGG CCGAGCCCCT CTGGGCCACC
GTCTTTGGCA GCCAGAGCCA CCACGAGGTC ACCATGTCCT ACCCCATGCG CTCCGTGCAG
CACCGGCACT TCCGCCTCGT GCACAACCTC AACTTCAAGA TGCCCTTTCC CATCGACCAG
GACTTCTACG TCTCACCCAC CTTCCAGGAC CTCCTGAACC GCACCACAGC TGGTCAGCCC
ACGGGCTGGT ACAAGGACCT CCGTCATTAC TACTACCGGG CGCGCTGGGA GCTCTACGAC
CGGAGCCGGG ACCCCCACGA GACCCAGAAC CTGGCCACCG ACCCGCGCTT TGCTCAGCTT
CTGGAGATGC TTCGGGACCA GCTGGCCAAG TGGCAGTGGG AGACCCACGA CCCCTGGGTG
TGCGCCCCCG ACGGCGTCCT GGAGGAGAAG CTCTCTCCCC AGTGCCAGCC CCTCCACAAT
GAGCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000190.1
CDS88..1596
Misc Feature(1)145..1419(+)
Misc Feature(2)154..1071(+)
Misc Feature(3)877..879(+)
Exon (1)1..175
Gene:SGSH
Gene Synonym:
Exon (2)176..336
Gene:SGSH
Gene Synonym:
Exon (3)337..442
Gene:SGSH
Gene Synonym:
Exon (4)443..593
Gene:SGSH
Gene Synonym:
Exon (5)594..750
Gene:SGSH
Gene Synonym:
Exon (6)751..832
Gene:SGSH
Gene Synonym:
Exon (7)833..1036
Gene:SGSH
Gene Synonym:
Exon (8)1037..2768
Gene:SGSH
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000199
Organism Homo sapiens (human)
Definition Homo sapiens N-sulfoglucosamine sulfohydrolase (SGSH), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000199

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCTGCC CCGTGCCCGC CTGCTGCGCG CTGCTGCTAG TCCTGGGGCT CTGCCGGGCG 
CGTCCCCGGA ACGCACTGCT GCTCCTCGCG GATGACGGAG GCTTTGAGAG TGGCGCGTAC
AACAACAGCG CCATCGCCAC CCCGCACCTG GACGCCTTGG CCCGCCGCAG CCTCCTCTTT
CGCAATGCCT TCACCTCGGT CAGCAGCTGC TCTCCCAGCC GCGCCAGCCT CCTCACTGGC
CTGCCCCAGC ATCAGAATGG GATGTACGGG CTGCACCAGG ACGTGCACCA CTTCAACTCC
TTCGACAAGG TGCGGAGCCT GCCGCTGCTG CTCAGCCAAG CTGGTGTGCG CACAGGCATC
ATCGGGAAGA AGCACGTGGG GCCGGAGACC GTGTACCCGT TTGACTTTGC GTACACGGAG
GAGAATGGCT CCGTCCTCCA GGTGGGGCGG AACATCACTA GAATTAAGCT GCTCGTCCGG
AAATTCCTGC AGACTCAGGA TGACCGGCCT TTCTTCCTCT ACGTCGCCTT CCACGACCCC
CACCGCTGTG GGCACTCCCA GCCCCAGTAC GGAACCTTCT GTGAGAAGTT TGGCAACGGA
GAGAGCGGCA TGGGTCGTAT CCCAGACTGG ACCCCCCAGG CCTACGACCC ACTGGACGTG
CTGGTGCCTT ACTTCGTCCC CAACACCCCG GCAGCCCGAG CCGACCTGGC CGCTCAGTAC
ACCACCGTCG GCCGCATGGA CCAAGGAGTT GGACTGGTGC TCCAGGAGCT GCGTGACGCC
GGTGTCCTGA ACGACACACT GGTGATCTTC ACGTCCGACA ACGGGATCCC CTTCCCCAGC
GGCAGGACCA ACCTGTACTG GCCGGGCACT GCTGAACCCT TACTGGTGTC ATCCCCGGAG
CACCCAAAAC GCTGGGGCCA AGTCAGCGAG GCCTACGTGA GCCTCCTAGA CCTCACGCCC
ACCATCTTGG ATTGGTTCTC GATCCCGTAC CCCAGCTACG CCATCTTTGG CTCGAAGACC
ATCCACCTCA CTGGCCGGTC CCTCCTGCCG GCGCTGGAGG CCGAGCCCCT CTGGGCCACC
GTCTTTGGCA GCCAGAGCCA CCACGAGGTC ACCATGTCCT ACCCCATGCG CTCCGTGCAG
CACCGGCACT TCCGCCTCGT GCACAACCTC AACTTCAAGA TGCCCTTTCC CATCGACCAG
GACTTCTACG TCTCACCCAC CTTCCAGGAC CTCCTGAACC GCACCACAGC TGGTCAGCCC
ACGGGCTGGT ACAAGGACCT CCGTCATTAC TACTACCGGG CGCGCTGGGA GCTCTACGAC
CGGAGCCGGG ACCCCCACGA GACCCAGAAC CTGGCCACCG ACCCGCGCTT TGCTCAGCTT
CTGGAGATGC TTCGGGACCA GCTGGCCAAG TGGCAGTGGG AGACCCACGA CCCCTGGGTG
TGCGCCCCCG ACGGCGTCCT GGAGGAGAAG CTCTCTCCCC AGTGCCAGCC CCTCCACAAT
GAGCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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