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SLC22A4 solute carrier family 22 (organic cation/zwitterion transporter), member 4 [Homo sapiens (human)]

Gene Symbol SLC22A4
Entrez Gene ID 6583
Full Name solute carrier family 22 (organic cation/zwitterion transporter), member 4
Synonyms OCTN1
General protein information
Preferred Names
solute carrier family 22 member 4
Names
solute carrier family 22 member 4
ET transporter
ergothioneine transporter
integral membrane transport protein
organic cation/carnitine transporter 1
solute carrier family 22 (organic cation/ergothioneine transporter), member 4
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

5

5q31.1

Summary Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. The encoded protein is an organic cation transporter and plasma integral membrane protein containing eleven putative transmembrane domains as well as a nucleotide-binding site motif. Transport by this protein is at least partially ATP-dependent. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

604190

Disorder Html: {Rheumatoid arthritis, susceptibility to}, 180300 (3)





The following SLC22A4 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC22A4 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu60625 XM_011543589 PREDICTED: Homo sapiens solute carrier family 22 (organic cation/zwitterion transporter), member 4 (SLC22A4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu39678 XM_006714675 PREDICTED: Homo sapiens solute carrier family 22 (organic cation/zwitterion transporter), member 4 (SLC22A4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $319
OHu19646 NM_003059 Homo sapiens solute carrier family 22 (organic cation/zwitterion transporter), member 4 (SLC22A4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$265.3
$379

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu60625
Accession Version XM_011543589.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1380bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product solute carrier family 22 member 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_034772.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCGGGACT ACGACGAGGT GATCGCCTTC CTGGGCGAGT GGGGGCCCTT CCAGCGCCTC 
ATCTTCTTCC TGCTCAGCGC CAGCATCATC CCCAATGGCT TCAATGGTAT GTCAGTCGTG
TTCCTGGCGG GGACCCCGGA GCACCGCTGT CGAGTGCCGG ACGCCGCGAA CCTGAGCAGC
GCCTGGCGCA ACAACAGTGT CCCGCTGCGG CTGCGGGACG GCCGCGAGGT GCCCCACAGC
TGCAGCCGCT ACCGGCTCGC CACCATCGCC AACTTCTCGG CGCTCGGGCT GGAGCCGGGG
CGCGACGTGG ACCTGGGGCA GCTGGAGCAG GAGAGCTGCC TGGATGGCTG GGAGTTCAGC
CAGGACGTCT ACCTGTCCAC CGTCGTGACC GAGGTTTGGC AGGAAGAACG TTCTCTTCGC
AACCATGGCT GTACAGACTG GCTTCAGCTT CCTGCAGATT TTCTCCATCA GCTGGGAGAT
GTTCACTGTG TTATTTGTCA TCGTGGGCAT GGGCCAGATC TCCAACTATG TGGTAGCCTT
CATACTAGGT TCATTCCTGA ATCTCCCCGA TGGCTGATAT CCCAGAGAAG ATTTAGAGAG
GCTGAAGATA TCATCCAAAA AGCTGCAAAA ATGAACAACA TAGCTGTACC AGCAGTGATA
TTTGATTCTG TGGAGGAGCT AAATCCCCTG AAGCAGCAGA AAGCTTTCAT TCTGGACCTG
TTCAGGACTC GGAATATTGC CATAATGACC ATTATGTCTT TGCTGCTATG GATGCTGACC
TCAGTGGGTT ACTTTGCTCT GTCTCTGGAT GCTCCTAATT TACATGGAGA TGCCTACCTG
AACTGTTTCC TCTCTGCCTT GATTGAAATT CCAGCTTACA TTACAGCCTG GCTGCTATTG
CGAACCCTGC CCAGGCGTTA TATCATAGCT GCAGTACTGT TCTGGGGAGG AGGTGTGCTT
CTCTTCATTC AACTGGTACC TGTGGATTAT TACTTCTTAT CCATTGGTCT GGTCATGCTG
GGAAAATTTG GGATCACCTC TGCTTTCTCC ATGCTGTATG TCTTCACTGC TGAGCTCTAC
CCAACCCTGG TCAGGAACAT GGCGGTGGGG GTCACATCCA CGGCCTCCAG AGTGGGCAGC
ATCATTGCCC CCTACTTTGT TTACCTCGGT GCTTACAACA GAATGCTGCC CTACATCGTC
ATGGGTAGTC TGACTGTCCT GATTGGAATC CTCACCCTTT TTTTCCCTGA AAGTTTGGGA
ATGACTCTTC CAGAAACCTT AGAGCAGATG CAGAAAGTGA AATGGTTCAG ATCTGGGAAA
AAAACAAGAG ACTCAATGGA GACAGAAGAA AATCCCAAGG TTCTAATAAC TGCATTCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011541891.1
CDS234..1613
Misc Feature(1)267..1511(+)
Misc Feature(2)981..1481(+)
Translation
Position Chain Variation Link
6 6 c, g dbSNP:551282344
19 19 a, g dbSNP:72557991
21 21 a, t dbSNP:536687222
45 45 c, t dbSNP:553184821
46 46 c, g, t dbSNP:751179159
50 50 a, g dbSNP:72557992
86 86 c, g dbSNP:367688187
92 92 a, g dbSNP:746117624
123 123 a, t dbSNP:567019637
124 124 g, t dbSNP:539139764
148 148 c, g dbSNP:558744755
161 161 c, t dbSNP:186843819
167 167 a, g dbSNP:544150842
184 184 a, g dbSNP:749661215
185 185 a, g, t dbSNP:768845505
188 188 a, g dbSNP:554877227
189 189 a, c dbSNP:574334825
194 194 a, g dbSNP:748600160
209 209 a, c dbSNP:191877372
215 215 a, c dbSNP:199926342
223 223 c, t dbSNP:760787423
224 224 a, t dbSNP:11568501
236 236 g, t dbSNP:776926210
238 238 a, g dbSNP:759385265
242 242 c, t dbSNP:764924955
250 250 a, g dbSNP:752743549
254 254 a, g dbSNP:762765767
254 254 -, g dbSNP:774010784
256 256 a, t dbSNP:763973178
258 258 c, g dbSNP:751103752
261 261 c, t dbSNP:756693505
270 270 a, g dbSNP:780840704
284 284 c, t dbSNP:750031077
288 288 c, t dbSNP:755438066
289 289 a, g dbSNP:779117302
295 295 -, tct dbSNP:771880004
305 305 a, g dbSNP:545500558
307 307 a, t dbSNP:758995324
309 309 a, g dbSNP:778384141
311 311 c, t dbSNP:747086200
314 314 c, t dbSNP:565099252
323 323 c, t dbSNP:777050701
328 328 a, g dbSNP:372949734
332 332 c, t dbSNP:769668837
337 337 a, g dbSNP:775173540
339 339 c, g dbSNP:762771154
353 353 g, t dbSNP:764024260
366 366 -, a dbSNP:772836216
367 367 c, t dbSNP:774216692
370 370 c, t dbSNP:761375217
371 371 a, g dbSNP:767014071
374 374 a, g, t dbSNP:750134122
391 391 c, t dbSNP:531041134
392 392 a, g, t dbSNP:375954722
398 398 a, c dbSNP:778433494
399 399 a, g dbSNP:747505093
414 414 a, g dbSNP:189072785
420 420 -, cgcaa dbSNP:766127231
420 420 a, c dbSNP:781099471
421 421 a, g dbSNP:567991307
436 436 c, t dbSNP:769942623
440 440 c, g dbSNP:775715637
442 442 g, t dbSNP:749095440
451 451 a, g dbSNP:768427012
452 452 c, t dbSNP:774343391
454 454 a, g dbSNP:761710621
468 468 c, t dbSNP:530039511
471 471 a, g dbSNP:767067005
477 477 a, g dbSNP:546926504
492 492 c, g dbSNP:760190076
495 495 a, c dbSNP:765948801
498 498 -, atcg dbSNP:776627155
498 498 a, c dbSNP:753468949
499 499 c, t dbSNP:758890712
501 501 c, g dbSNP:764548525
518 518 c, t dbSNP:752151457
519 519 c, g dbSNP:757815454
520 520 a, g dbSNP:781696756
534 534 c, t dbSNP:750475535
543 543 g, t dbSNP:756161236
546 546 c, g dbSNP:780199805
559 559 a, g dbSNP:749511874
571 571 a, g dbSNP:768484124
572 572 c, t dbSNP:11568502
575 575 c, g dbSNP:747891999
588 588 g, t dbSNP:771932077
605 605 a, c dbSNP:773132721
608 608 g, t dbSNP:760113387
617 617 c, g, t dbSNP:770458543
618 618 a, g dbSNP:755377506
623 623 c, g dbSNP:764601594
626 626 a, g dbSNP:751992452
634 634 a, g dbSNP:371708747
642 642 c, t dbSNP:771320444
643 643 a, c, g dbSNP:781510670
651 651 c, g, t dbSNP:374839569
652 652 a, g dbSNP:144031153
663 663 c, t dbSNP:768616452
664 664 a, g dbSNP:774674579
669 669 c, g dbSNP:456392
674 674 a, g dbSNP:369071242
684 684 c, t dbSNP:460415
694 694 c, t dbSNP:767803439
696 696 c, t dbSNP:750278877
698 698 c, g dbSNP:760482890
702 702 c, t dbSNP:766356847
713 713 c, t dbSNP:753702919
720 720 a, t dbSNP:754580524
725 725 a, t dbSNP:147297796
728 728 c, t dbSNP:752392368
735 735 c, t dbSNP:11568498
736 736 a, g dbSNP:777364340
743 743 c, t dbSNP:746234716
744 744 a, g dbSNP:11568500
758 758 a, g dbSNP:373520579
759 759 c, g, t dbSNP:143879161
762 762 c, t dbSNP:774318267
763 763 a, g dbSNP:148604311
768 768 a, g dbSNP:376697042
770 770 c, t dbSNP:773512581
771 771 a, c dbSNP:199611329
772 772 g, t dbSNP:369732593
801 801 c, t dbSNP:11568503
802 802 a, g dbSNP:748090484
809 809 g, t dbSNP:771527346
811 811 c, t dbSNP:769645496
814 814 c, g dbSNP:772796676
831 831 a, g dbSNP:746475008
845 845 c, t dbSNP:770708660
849 849 a, c dbSNP:776363804
850 850 a, c dbSNP:763467165
859 859 a, c dbSNP:201521490
869 869 c, t dbSNP:774759030
874 874 c, t dbSNP:272893
882 882 c, t dbSNP:767872488
894 894 c, t dbSNP:750523509
919 919 c, t dbSNP:140888297
920 920 a, c, t dbSNP:200185233
922 922 c, ct, t dbSNP:72552719
922 922 g, t dbSNP:770551085
932 932 a, g dbSNP:541361427
959 959 a, g dbSNP:367863918
961 961 c, t dbSNP:768949924
963 963 c, t dbSNP:76343123
964 964 a, g dbSNP:762018264
972 972 a, c, g dbSNP:533127429
988 988 a, t dbSNP:567645504
989 989 a, g dbSNP:760813238
1001 1001 a, g dbSNP:766418958
1042 1042 a, t dbSNP:752847081
1050 1050 a, g dbSNP:150157940
1056 1056 c, t dbSNP:762870150
1059 1059 a, g dbSNP:575659970
1060 1060 a, g dbSNP:751596030
1071 1071 c, g dbSNP:757282135
1076 1076 c, t dbSNP:781209544
1083 1083 c, t dbSNP:191121049
1131 1131 c, t dbSNP:372962746
1133 1133 a, g dbSNP:779825520
1134 1134 c, t dbSNP:748864045
1135 1135 a, g dbSNP:772540855
1137 1137 a, t dbSNP:778137526
1138 1138 c, t dbSNP:201507559
1139 1139 c, g dbSNP:272879
1140 1140 c, g dbSNP:1050150
1142 1142 a, g dbSNP:148936987
1149 1149 c, t dbSNP:201371114
1150 1150 a, g dbSNP:769756304
1153 1153 a, g dbSNP:775819832
1190 1190 c, g dbSNP:17852518
1196 1196 c, g, t dbSNP:779525039
1204 1204 a, c dbSNP:751405490
1214 1214 c, t dbSNP:761644362
1218 1218 a, g dbSNP:767621862
1219 1219 -, att dbSNP:561053135
1235 1235 a, c dbSNP:377673823
1242 1242 c, t dbSNP:760555279
1248 1248 a, g dbSNP:147032697
1258 1258 a, c dbSNP:753288548
1261 1261 g, t dbSNP:754423266
1265 1265 a, g dbSNP:764924147
1268 1268 a, c dbSNP:529910545
1271 1271 c, t dbSNP:757655893
1273 1273 c, g dbSNP:781386691
1283 1283 a, c dbSNP:746193557
1292 1292 c, t dbSNP:756609362
1300 1300 a, c dbSNP:780552167
1304 1304 c, t dbSNP:370947767
1307 1307 a, g dbSNP:193043140
1309 1309 c, t dbSNP:774755477
1311 1311 c, t dbSNP:748361558
1312 1312 a, t dbSNP:772374561
1314 1314 c, g dbSNP:772915647
1318 1318 c, t dbSNP:760456007
1320 1320 c, g dbSNP:138433908
1322 1322 g, t dbSNP:776614713
1327 1327 a, g dbSNP:759110538
1328 1328 g, t dbSNP:764692894
1329 1329 a, c dbSNP:752394406
1336 1336 c, t dbSNP:200543879
1337 1337 a, c, g dbSNP:199597043
1339 1339 -, ggg dbSNP:373747100
1339 1339 g, t dbSNP:202244640
1342 1342 a, g dbSNP:4646201
1345 1345 g, t dbSNP:200741625
1347 1347 a, g dbSNP:143888295
1348 1348 c, t dbSNP:754367405
1350 1350 c, t dbSNP:530272146
1352 1352 a, c dbSNP:755159592
1354 1354 c, t dbSNP:779159133
1355 1355 a, g dbSNP:201228074
1364 1364 a, t dbSNP:772432542
1369 1369 a, g dbSNP:777989435
1370 1370 c, g dbSNP:12777
1396 1396 a, g dbSNP:770752876
1400 1400 c, t dbSNP:183075585
1401 1401 a, g dbSNP:148341373
1412 1412 a, c dbSNP:775348982
1417 1417 c, t dbSNP:557926964
1428 1428 a, g dbSNP:375225322
1430 1430 c, t dbSNP:141587824
1431 1431 a, g dbSNP:188858026
1432 1432 a, t dbSNP:767034234
1433 1433 c, t dbSNP:566960067
1435 1435 c, t dbSNP:375398349
1449 1449 a, g dbSNP:765906886
1450 1450 c, t dbSNP:753255483
1457 1457 c, t dbSNP:61731074
1462 1462 c, t dbSNP:764214038
1463 1463 a, c dbSNP:57512142
1464 1464 c, t dbSNP:1050152
1466 1466 a, c dbSNP:201450794
1470 1470 -, t dbSNP:776307267
1471 1471 -, t dbSNP:770747109
1477 1477 c, t dbSNP:745677209
1477 1477 -, t dbSNP:72552721
1483 1483 a, t dbSNP:755898461
1499 1499 c, t dbSNP:535181187
1500 1500 a, c, t dbSNP:558087632
1514 1514 a, c dbSNP:749225329
1515 1515 c, g dbSNP:201132879
1524 1524 a, c dbSNP:773940777
1526 1526 a, g dbSNP:146182196
1537 1537 a, c, g dbSNP:771861252
1550 1550 -, a dbSNP:759406751
1565 1565 c, t dbSNP:748994718
1580 1580 a, g dbSNP:754945336
1581 1581 a, g dbSNP:778655134
1582 1582 a, g dbSNP:747748208
1604 1604 c, t dbSNP:116837840
1616 1616 a, c, g dbSNP:11568508
1644 1644 a, g dbSNP:770661459
1645 1645 a, g dbSNP:775956581
1661 1661 a, c dbSNP:763161391
1700 1700 c, t dbSNP:545290223
1706 1706 c, t dbSNP:139332157
1709 1709 a, c, t dbSNP:148289043
1747 1747 c, t dbSNP:560593165
1764 1764 a, g dbSNP:777865006
1780 1780 a, g dbSNP:761506580
1782 1782 c, t dbSNP:532865558
1832 1832 a, c dbSNP:143426016
1882 1882 c, t dbSNP:371985838
1891 1891 c, t dbSNP:771241244
1915 1915 c, t dbSNP:112601131

Target ORF information:

RefSeq Version XM_011543589
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens solute carrier family 22 (organic cation/zwitterion transporter), member 4 (SLC22A4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543589

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCGGGACT ACGACGAGGT GATCGCCTTC CTGGGCGAGT GGGGGCCCTT CCAGCGCCTC 
ATCTTCTTCC TGCTCAGCGC CAGCATCATC CCCAATGGCT TCAATGGTAT GTCAGTCGTG
TTCCTGGCGG GGACCCCGGA GCACCGCTGT CGAGTGCCGG ACGCCGCGAA CCTGAGCAGC
GCCTGGCGCA ACAACAGTGT CCCGCTGCGG CTGCGGGACG GCCGCGAGGT GCCCCACAGC
TGCAGCCGCT ACCGGCTCGC CACCATCGCC AACTTCTCGG CGCTCGGGCT GGAGCCGGGG
CGCGACGTGG ACCTGGGGCA GCTGGAGCAG GAGAGCTGCC TGGATGGCTG GGAGTTCAGC
CAGGACGTCT ACCTGTCCAC CGTCGTGACC GAGGTTTGGC AGGAAGAACG TTCTCTTCGC
AACCATGGCT GTACAGACTG GCTTCAGCTT CCTGCAGATT TTCTCCATCA GCTGGGAGAT
GTTCACTGTG TTATTTGTCA TCGTGGGCAT GGGCCAGATC TCCAACTATG TGGTAGCCTT
CATACTAGGT TCATTCCTGA ATCTCCCCGA TGGCTGATAT CCCAGAGAAG ATTTAGAGAG
GCTGAAGATA TCATCCAAAA AGCTGCAAAA ATGAACAACA TAGCTGTACC AGCAGTGATA
TTTGATTCTG TGGAGGAGCT AAATCCCCTG AAGCAGCAGA AAGCTTTCAT TCTGGACCTG
TTCAGGACTC GGAATATTGC CATAATGACC ATTATGTCTT TGCTGCTATG GATGCTGACC
TCAGTGGGTT ACTTTGCTCT GTCTCTGGAT GCTCCTAATT TACATGGAGA TGCCTACCTG
AACTGTTTCC TCTCTGCCTT GATTGAAATT CCAGCTTACA TTACAGCCTG GCTGCTATTG
CGAACCCTGC CCAGGCGTTA TATCATAGCT GCAGTACTGT TCTGGGGAGG AGGTGTGCTT
CTCTTCATTC AACTGGTACC TGTGGATTAT TACTTCTTAT CCATTGGTCT GGTCATGCTG
GGAAAATTTG GGATCACCTC TGCTTTCTCC ATGCTGTATG TCTTCACTGC TGAGCTCTAC
CCAACCCTGG TCAGGAACAT GGCGGTGGGG GTCACATCCA CGGCCTCCAG AGTGGGCAGC
ATCATTGCCC CCTACTTTGT TTACCTCGGT GCTTACAACA GAATGCTGCC CTACATCGTC
ATGGGTAGTC TGACTGTCCT GATTGGAATC CTCACCCTTT TTTTCCCTGA AAGTTTGGGA
ATGACTCTTC CAGAAACCTT AGAGCAGATG CAGAAAGTGA AATGGTTCAG ATCTGGGAAA
AAAACAAGAG ACTCAATGGA GACAGAAGAA AATCCCAAGG TTCTAATAAC TGCATTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu39678
Accession Version XM_006714675.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1128bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product solute carrier family 22 member 4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_034772.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578810506. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGTAC AGACTGGCTT CAGCTTCCTG CAGATTTTCT CCATCAGCTG GGAGATGTTC 
ACTGTGTTAT TTGTCATCGT GGGCATGGGC CAGATCTCCA ACTATGTGGT AGCCTTCATA
CTAGGAACAG AAATTCTTGG CAAGTCAGTT CGTATTATAT TCTCTACATT AGGAGTGTGC
ACATTTTTTG CAGTTGGCTA TATGCTGCTG CCACTGTTTG CTTACTTCAT CAGAGACTGG
CGGATGCTGC TGCTGGCGCT GACGGTGCCG GGAGTGCTGT GTGTCCCGCT GTGGTGGTTC
ATTCCTGAAT CTCCCCGATG GCTGATATCC CAGAGAAGAT TTAGAGAGGC TGAAGATATC
ATCCAAAAAG CTGCAAAAAT GAACAACATA GCTGTACCAG CAGTGATATT TGATTCTGTG
GAGGAGCTAA ATCCCCTGAA GCAGCAGAAA GCTTTCATTC TGGACCTGTT CAGGACTCGG
AATATTGCCA TAATGACCAT TATGTCTTTG CTGCTATGGA TGCTGACCTC AGTGGGTTAC
TTTGCTCTGT CTCTGGATGC TCCTAATTTA CATGGAGATG CCTACCTGAA CTGTTTCCTC
TCTGCCTTGA TTGAAATTCC AGCTTACATT ACAGCCTGGC TGCTATTGCG AACCCTGCCC
AGGCGTTATA TCATAGCTGC AGTACTGTTC TGGGGAGGAG GTGTGCTTCT CTTCATTCAA
CTGGTACCTG TGGATTATTA CTTCTTATCC ATTGGTCTGG TCATGCTGGG AAAATTTGGG
ATCACCTCTG CTTTCTCCAT GCTGTATGTC TTCACTGCTG AGCTCTACCC AACCCTGGTC
AGGAACATGG CGGTGGGGGT CACATCCACG GCCTCCAGAG TGGGCAGCAT CATTGCCCCC
TACTTTGTTT ACCTCGGTGC TTACAACAGA ATGCTGCCCT ACATCGTCAT GGGTAGTCTG
ACTGTCCTGA TTGGAATCCT CACCCTTTTT TTCCCTGAAA GTTTGGGAAT GACTCTTCCA
GAAACCTTAG AGCAGATGCA GAAAGTGAAA TGGTTCAGAT CTGGGAAAAA AACAAGAGAC
TCAATGGAGA CAGAAGAAAA TCCCAAGGTT CTAATAACTG CATTCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006714738.1
CDS115..1242
Misc Feature(1)115..1110(+)
Misc Feature(2)205..1002(+)
Translation
Position Chain Variation Link
3 3 c, g dbSNP:780199805
16 16 a, g dbSNP:749511874
28 28 a, g dbSNP:768484124
29 29 c, t dbSNP:11568502
32 32 c, g dbSNP:747891999
45 45 g, t dbSNP:771932077
62 62 a, c dbSNP:773132721
65 65 g, t dbSNP:760113387
74 74 c, g, t dbSNP:770458543
75 75 a, g dbSNP:755377506
80 80 c, g dbSNP:764601594
83 83 a, g dbSNP:751992452
91 91 a, g dbSNP:371708747
99 99 c, t dbSNP:771320444
100 100 a, c, g dbSNP:781510670
108 108 c, g, t dbSNP:374839569
109 109 a, g dbSNP:144031153
120 120 c, t dbSNP:768616452
121 121 a, g dbSNP:774674579
126 126 c, g dbSNP:456392
131 131 a, g dbSNP:369071242
141 141 c, t dbSNP:460415
151 151 c, t dbSNP:767803439
153 153 c, t dbSNP:750278877
155 155 c, g dbSNP:760482890
159 159 c, t dbSNP:766356847
170 170 c, t dbSNP:753702919
177 177 a, t dbSNP:754580524
182 182 a, t dbSNP:147297796
185 185 c, t dbSNP:752392368
192 192 c, t dbSNP:11568498
193 193 a, g dbSNP:777364340
200 200 c, t dbSNP:746234716
201 201 a, g dbSNP:11568500
215 215 a, g dbSNP:373520579
216 216 c, g, t dbSNP:143879161
219 219 c, t dbSNP:774318267
220 220 a, g dbSNP:148604311
225 225 a, g dbSNP:376697042
227 227 c, t dbSNP:773512581
228 228 a, c dbSNP:199611329
229 229 g, t dbSNP:369732593
257 257 a, g dbSNP:765091021
261 261 a, g dbSNP:775346954
263 263 c, t dbSNP:201467740
265 265 c, t dbSNP:375988789
266 266 a, c, g dbSNP:143140136
272 272 c, t dbSNP:756882520
282 282 a, g dbSNP:767158433
285 285 a, t dbSNP:146705097
289 289 a, g dbSNP:755317078
305 305 c, t dbSNP:575829481
308 308 a, t dbSNP:541967934
318 318 a, g dbSNP:758587614
321 321 a, g dbSNP:146340469
327 327 a, g dbSNP:747058771
328 328 a, c dbSNP:771085988
330 330 c, g dbSNP:751368946
333 333 c, t dbSNP:776844663
343 343 a, g dbSNP:372778380
346 346 a, c dbSNP:376295543
351 351 c, t dbSNP:370379416
352 352 c, t dbSNP:762856391
354 354 c, g dbSNP:368695578
355 355 a, c, t dbSNP:768533971
366 366 a, g dbSNP:761322837
371 371 c, t dbSNP:767093107
375 375 c, g dbSNP:200918264
377 377 c, t dbSNP:139188733
378 378 a, g dbSNP:144122563
383 383 c, t dbSNP:199535716
384 384 a, g dbSNP:371002294
388 388 c, g dbSNP:758993571
397 397 c, g dbSNP:375525577
398 398 c, t dbSNP:751593840
401 401 c, t dbSNP:757302889
406 406 c, t dbSNP:368056012
430 430 c, t dbSNP:11568503
431 431 a, g dbSNP:748090484
438 438 g, t dbSNP:771527346
440 440 c, t dbSNP:769645496
443 443 c, g dbSNP:772796676
460 460 a, g dbSNP:746475008
474 474 c, t dbSNP:770708660
478 478 a, c dbSNP:776363804
479 479 a, c dbSNP:763467165
488 488 a, c dbSNP:201521490
498 498 c, t dbSNP:774759030
503 503 c, t dbSNP:272893
511 511 c, t dbSNP:767872488
523 523 c, t dbSNP:750523509
548 548 c, t dbSNP:140888297
549 549 a, c, t dbSNP:200185233
551 551 c, ct, t dbSNP:72552719
551 551 g, t dbSNP:770551085
561 561 a, g dbSNP:541361427
588 588 a, g dbSNP:367863918
590 590 c, t dbSNP:768949924
592 592 c, t dbSNP:76343123
593 593 a, g dbSNP:762018264
601 601 a, c, g dbSNP:533127429
617 617 a, t dbSNP:567645504
618 618 a, g dbSNP:760813238
630 630 a, g dbSNP:766418958
671 671 a, t dbSNP:752847081
679 679 a, g dbSNP:150157940
685 685 c, t dbSNP:762870150
688 688 a, g dbSNP:575659970
689 689 a, g dbSNP:751596030
700 700 c, g dbSNP:757282135
705 705 c, t dbSNP:781209544
712 712 c, t dbSNP:191121049
760 760 c, t dbSNP:372962746
762 762 a, g dbSNP:779825520
763 763 c, t dbSNP:748864045
764 764 a, g dbSNP:772540855
766 766 a, t dbSNP:778137526
767 767 c, t dbSNP:201507559
768 768 c, g dbSNP:272879
769 769 c, g dbSNP:1050150
771 771 a, g dbSNP:148936987
778 778 c, t dbSNP:201371114
779 779 a, g dbSNP:769756304
782 782 a, g dbSNP:775819832
819 819 c, g dbSNP:17852518
825 825 c, g, t dbSNP:779525039
833 833 a, c dbSNP:751405490
843 843 c, t dbSNP:761644362
847 847 a, g dbSNP:767621862
848 848 -, att dbSNP:561053135
864 864 a, c dbSNP:377673823
871 871 c, t dbSNP:760555279
877 877 a, g dbSNP:147032697
887 887 a, c dbSNP:753288548
890 890 g, t dbSNP:754423266
894 894 a, g dbSNP:764924147
897 897 a, c dbSNP:529910545
900 900 c, t dbSNP:757655893
902 902 c, g dbSNP:781386691
912 912 a, c dbSNP:746193557
921 921 c, t dbSNP:756609362
929 929 a, c dbSNP:780552167
933 933 c, t dbSNP:370947767
936 936 a, g dbSNP:193043140
938 938 c, t dbSNP:774755477
940 940 c, t dbSNP:748361558
941 941 a, t dbSNP:772374561
943 943 c, g dbSNP:772915647
947 947 c, t dbSNP:760456007
949 949 c, g dbSNP:138433908
951 951 g, t dbSNP:776614713
956 956 a, g dbSNP:759110538
957 957 g, t dbSNP:764692894
958 958 a, c dbSNP:752394406
965 965 c, t dbSNP:200543879
966 966 a, c, g dbSNP:199597043
968 968 -, ggg dbSNP:373747100
968 968 g, t dbSNP:202244640
971 971 a, g dbSNP:4646201
974 974 g, t dbSNP:200741625
976 976 a, g dbSNP:143888295
977 977 c, t dbSNP:754367405
979 979 c, t dbSNP:530272146
981 981 a, c dbSNP:755159592
983 983 c, t dbSNP:779159133
984 984 a, g dbSNP:201228074
993 993 a, t dbSNP:772432542
998 998 a, g dbSNP:777989435
999 999 c, g dbSNP:12777
1025 1025 a, g dbSNP:770752876
1029 1029 c, t dbSNP:183075585
1030 1030 a, g dbSNP:148341373
1041 1041 a, c dbSNP:775348982
1046 1046 c, t dbSNP:557926964
1057 1057 a, g dbSNP:375225322
1059 1059 c, t dbSNP:141587824
1060 1060 a, g dbSNP:188858026
1061 1061 a, t dbSNP:767034234
1062 1062 c, t dbSNP:566960067
1064 1064 c, t dbSNP:375398349
1078 1078 a, g dbSNP:765906886
1079 1079 c, t dbSNP:753255483
1086 1086 c, t dbSNP:61731074
1091 1091 c, t dbSNP:764214038
1092 1092 a, c dbSNP:57512142
1093 1093 c, t dbSNP:1050152
1095 1095 a, c dbSNP:201450794
1099 1099 -, t dbSNP:776307267
1100 1100 -, t dbSNP:770747109
1106 1106 c, t dbSNP:745677209
1106 1106 -, t dbSNP:72552721
1112 1112 a, t dbSNP:755898461
1128 1128 c, t dbSNP:535181187
1129 1129 a, c, t dbSNP:558087632
1143 1143 a, c dbSNP:749225329
1144 1144 c, g dbSNP:201132879
1153 1153 a, c dbSNP:773940777
1155 1155 a, g dbSNP:146182196
1166 1166 a, c, g dbSNP:771861252
1179 1179 -, a dbSNP:759406751
1194 1194 c, t dbSNP:748994718
1209 1209 a, g dbSNP:754945336
1210 1210 a, g dbSNP:778655134
1211 1211 a, g dbSNP:747748208
1233 1233 c, t dbSNP:116837840
1245 1245 a, c, g dbSNP:11568508
1273 1273 a, g dbSNP:770661459
1274 1274 a, g dbSNP:775956581
1290 1290 a, c dbSNP:763161391
1329 1329 c, t dbSNP:545290223
1335 1335 c, t dbSNP:139332157
1338 1338 a, c, t dbSNP:148289043
1376 1376 c, t dbSNP:560593165
1393 1393 a, g dbSNP:777865006
1409 1409 a, g dbSNP:761506580
1411 1411 c, t dbSNP:532865558
1461 1461 a, c dbSNP:143426016
1511 1511 c, t dbSNP:371985838
1520 1520 c, t dbSNP:771241244
1544 1544 c, t dbSNP:112601131

Target ORF information:

RefSeq Version XM_006714675
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens solute carrier family 22 (organic cation/zwitterion transporter), member 4 (SLC22A4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006714675

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGTAC AGACTGGCTT CAGCTTCCTG CAGATTTTCT CCATCAGCTG GGAGATGTTC 
ACTGTGTTAT TTGTCATCGT GGGCATGGGC CAGATCTCCA ACTATGTGGT AGCCTTCATA
CTAGGAACAG AAATTCTTGG CAAGTCAGTT CGTATTATAT TCTCTACATT AGGAGTGTGC
ACATTTTTTG CAGTTGGCTA TATGCTGCTG CCACTGTTTG CTTACTTCAT CAGAGACTGG
CGGATGCTGC TGCTGGCGCT GACGGTGCCG GGAGTGCTGT GTGTCCCGCT GTGGTGGTTC
ATTCCTGAAT CTCCCCGATG GCTGATATCC CAGAGAAGAT TTAGAGAGGC TGAAGATATC
ATCCAAAAAG CTGCAAAAAT GAACAACATA GCTGTACCAG CAGTGATATT TGATTCTGTG
GAGGAGCTAA ATCCCCTGAA GCAGCAGAAA GCTTTCATTC TGGACCTGTT CAGGACTCGG
AATATTGCCA TAATGACCAT TATGTCTTTG CTGCTATGGA TGCTGACCTC AGTGGGTTAC
TTTGCTCTGT CTCTGGATGC TCCTAATTTA CATGGAGATG CCTACCTGAA CTGTTTCCTC
TCTGCCTTGA TTGAAATTCC AGCTTACATT ACAGCCTGGC TGCTATTGCG AACCCTGCCC
AGGCGTTATA TCATAGCTGC AGTACTGTTC TGGGGAGGAG GTGTGCTTCT CTTCATTCAA
CTGGTACCTG TGGATTATTA CTTCTTATCC ATTGGTCTGG TCATGCTGGG AAAATTTGGG
ATCACCTCTG CTTTCTCCAT GCTGTATGTC TTCACTGCTG AGCTCTACCC AACCCTGGTC
AGGAACATGG CGGTGGGGGT CACATCCACG GCCTCCAGAG TGGGCAGCAT CATTGCCCCC
TACTTTGTTT ACCTCGGTGC TTACAACAGA ATGCTGCCCT ACATCGTCAT GGGTAGTCTG
ACTGTCCTGA TTGGAATCCT CACCCTTTTT TTCCCTGAAA GTTTGGGAAT GACTCTTCCA
GAAACCTTAG AGCAGATGCA GAAAGTGAAA TGGTTCAGAT CTGGGAAAAA AACAAGAGAC
TCAATGGAGA CAGAAGAAAA TCCCAAGGTT CTAATAACTG CATTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19646
Accession Version NM_003059.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1656bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product solute carrier family 22 member 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC028313.1. This sequence is a reference standard in the RefSeqGene project. On Nov 3, 2002 this sequence version replaced gi:4507002. Summary: Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. The encoded protein is an organic cation transporter and plasma integral membrane protein containing eleven putative transmembrane domains as well as a nucleotide-binding site motif. Transport by this protein is at least partially ATP-dependent. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028313.1, Y09881.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCGGGACT ACGACGAGGT GATCGCCTTC CTGGGCGAGT GGGGGCCCTT CCAGCGCCTC 
ATCTTCTTCC TGCTCAGCGC CAGCATCATC CCCAATGGCT TCAATGGTAT GTCAGTCGTG
TTCCTGGCGG GGACCCCGGA GCACCGCTGT CGAGTGCCGG ACGCCGCGAA CCTGAGCAGC
GCCTGGCGCA ACAACAGTGT CCCGCTGCGG CTGCGGGACG GCCGCGAGGT GCCCCACAGC
TGCAGCCGCT ACCGGCTCGC CACCATCGCC AACTTCTCGG CGCTCGGGCT GGAGCCGGGG
CGCGACGTGG ACCTGGGGCA GCTGGAGCAG GAGAGCTGCC TGGATGGCTG GGAGTTCAGC
CAGGACGTCT ACCTGTCCAC CGTCGTGACC GAGTGGAATC TGGTGTGTGA GGACAACTGG
AAGGTGCCCC TCACCACCTC CCTGTTCTTC GTAGGCGTGC TCCTCGGCTC CTTCGTGTCC
GGGCAGCTGT CAGACAGGTT TGGCAGGAAG AACGTTCTCT TCGCAACCAT GGCTGTACAG
ACTGGCTTCA GCTTCCTGCA GATTTTCTCC ATCAGCTGGG AGATGTTCAC TGTGTTATTT
GTCATCGTGG GCATGGGCCA GATCTCCAAC TATGTGGTAG CCTTCATACT AGGAACAGAA
ATTCTTGGCA AGTCAGTTCG TATTATATTC TCTACATTAG GAGTGTGCAC ATTTTTTGCA
GTTGGCTATA TGCTGCTGCC ACTGTTTGCT TACTTCATCA GAGACTGGCG GATGCTGCTG
CTGGCGCTGA CGGTGCCGGG AGTGCTGTGT GTCCCGCTGT GGTGGTTCAT TCCTGAATCT
CCCCGATGGC TGATATCCCA GAGAAGATTT AGAGAGGCTG AAGATATCAT CCAAAAAGCT
GCAAAAATGA ACAACATAGC TGTACCAGCA GTGATATTTG ATTCTGTGGA GGAGCTAAAT
CCCCTGAAGC AGCAGAAAGC TTTCATTCTG GACCTGTTCA GGACTCGGAA TATTGCCATA
ATGACCATTA TGTCTTTGCT GCTATGGATG CTGACCTCAG TGGGTTACTT TGCTCTGTCT
CTGGATGCTC CTAATTTACA TGGAGATGCC TACCTGAACT GTTTCCTCTC TGCCTTGATT
GAAATTCCAG CTTACATTAC AGCCTGGCTG CTATTGCGAA CCCTGCCCAG GCGTTATATC
ATAGCTGCAG TACTGTTCTG GGGAGGAGGT GTGCTTCTCT TCATTCAACT GGTACCTGTG
GATTATTACT TCTTATCCAT TGGTCTGGTC ATGCTGGGAA AATTTGGGAT CACCTCTGCT
TTCTCCATGC TGTATGTCTT CACTGCTGAG CTCTACCCAA CCCTGGTCAG GAACATGGCG
GTGGGGGTCA CATCCACGGC CTCCAGAGTG GGCAGCATCA TTGCCCCCTA CTTTGTTTAC
CTCGGTGCTT ACAACAGAAT GCTGCCCTAC ATCGTCATGG GTAGTCTGAC TGTCCTGATT
GGAATCCTCA CCCTTTTTTT CCCTGAAAGT TTGGGAATGA CTCTTCCAGA AACCTTAGAG
CAGATGCAGA AAGTGAAATG GTTCAGATCT GGGAAAAAAA CAAGAGACTC AATGGAGACA
GAAGAAAATC CCAAGGTTCT AATAACTGCA TTCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_003050.2
CDS166..1821
Misc Feature(1)73..75(+)
Misc Feature(2)199..1719(+)
Misc Feature(3)226..288(+)
Misc Feature(4)532..1689(+)
Misc Feature(5)532..1581(+)
Misc Feature(6)589..651(+)
Misc Feature(7)679..741(+)
Misc Feature(8)757..819(+)
Misc Feature(9)862..924(+)
Misc Feature(10)937..999(+)
Misc Feature(11)1177..1239(+)
Misc Feature(12)1279..1341(+)
Misc Feature(13)1363..1425(+)
Misc Feature(14)1444..1506(+)
Misc Feature(15)1546..1608(+)
Misc Feature(16)1624..1686(+)
Exon (1)1..558
Gene:SLC22A4
Gene Synonym:
Exon (2)559..662
Gene:SLC22A4
Gene Synonym:
Exon (3)663..817
Gene:SLC22A4
Gene Synonym:
Exon (4)818..989
Gene:SLC22A4
Gene Synonym:
Exon (5)990..1116
Gene:SLC22A4
Gene Synonym:
Exon (6)1117..1211
Gene:SLC22A4
Gene Synonym:
Exon (7)1212..1426
Gene:SLC22A4
Gene Synonym:
Exon (8)1427..1609
Gene:SLC22A4
Gene Synonym:
Exon (9)1610..1745
Gene:SLC22A4
Gene Synonym:
Exon (10)1746..2192
Gene:SLC22A4
Gene Synonym:
Translation
Position Chain Variation Link
18 18 c, g dbSNP:367688187
24 24 a, g dbSNP:746117624
55 55 a, t dbSNP:567019637
56 56 g, t dbSNP:539139764
80 80 c, g dbSNP:558744755
93 93 c, t dbSNP:186843819
99 99 a, g dbSNP:544150842
116 116 a, g dbSNP:749661215
117 117 a, g, t dbSNP:768845505
120 120 a, g dbSNP:554877227
121 121 a, c dbSNP:574334825
126 126 a, g dbSNP:748600160
141 141 a, c dbSNP:191877372
147 147 a, c dbSNP:199926342
155 155 c, t dbSNP:760787423
156 156 a, t dbSNP:11568501
168 168 g, t dbSNP:776926210
170 170 a, g dbSNP:759385265
174 174 c, t dbSNP:764924955
182 182 a, g dbSNP:752743549
186 186 a, g dbSNP:762765767
186 186 -, g dbSNP:774010784
188 188 a, t dbSNP:763973178
190 190 c, g dbSNP:751103752
193 193 c, t dbSNP:756693505
202 202 a, g dbSNP:780840704
216 216 c, t dbSNP:750031077
220 220 c, t dbSNP:755438066
221 221 a, g dbSNP:779117302
227 227 -, tct dbSNP:771880004
237 237 a, g dbSNP:545500558
239 239 a, t dbSNP:758995324
241 241 a, g dbSNP:778384141
243 243 c, t dbSNP:747086200
246 246 c, t dbSNP:565099252
255 255 c, t dbSNP:777050701
260 260 a, g dbSNP:372949734
264 264 c, t dbSNP:769668837
269 269 a, g dbSNP:775173540
271 271 c, g dbSNP:762771154
285 285 g, t dbSNP:764024260
298 298 -, a dbSNP:772836216
299 299 c, t dbSNP:774216692
302 302 c, t dbSNP:761375217
303 303 a, g dbSNP:767014071
306 306 a, g, t dbSNP:750134122
323 323 c, t dbSNP:531041134
324 324 a, g, t dbSNP:375954722
330 330 a, c dbSNP:778433494
331 331 a, g dbSNP:747505093
346 346 a, g dbSNP:189072785
352 352 -, cgcaa dbSNP:766127231
352 352 a, c dbSNP:781099471
353 353 a, g dbSNP:567991307
368 368 c, t dbSNP:769942623
372 372 c, g dbSNP:775715637
374 374 g, t dbSNP:749095440
383 383 a, g dbSNP:768427012
384 384 c, t dbSNP:774343391
386 386 a, g dbSNP:761710621
400 400 c, t dbSNP:530039511
403 403 a, g dbSNP:767067005
409 409 a, g dbSNP:546926504
424 424 c, g dbSNP:760190076
427 427 a, c dbSNP:765948801
430 430 -, atcg dbSNP:776627155
430 430 a, c dbSNP:753468949
431 431 c, t dbSNP:758890712
433 433 c, g dbSNP:764548525
450 450 c, t dbSNP:752151457
451 451 c, g dbSNP:757815454
452 452 a, g dbSNP:781696756
466 466 c, t dbSNP:750475535
475 475 g, t dbSNP:756161236
478 478 c, g dbSNP:780199805
491 491 a, g dbSNP:749511874
503 503 a, g dbSNP:768484124
504 504 c, t dbSNP:11568502
507 507 c, g dbSNP:747891999
520 520 g, t dbSNP:771932077
537 537 a, c dbSNP:773132721
540 540 g, t dbSNP:760113387
549 549 c, g, t dbSNP:770458543
550 550 a, g dbSNP:755377506
555 555 c, g dbSNP:764601594
558 558 a, g dbSNP:751992452
561 561 g, t dbSNP:149111296
570 570 a, g dbSNP:745386993
572 572 a, g dbSNP:368904414
579 579 a, c dbSNP:769416460
588 588 a, g dbSNP:775350544
592 592 c, t dbSNP:748940410
593 593 c, t dbSNP:772375048
598 598 a, g dbSNP:773652433
602 602 c, g dbSNP:760911652
607 607 -, ct dbSNP:765247850
607 607 c, g dbSNP:398064
608 608 c, t dbSNP:766879151
615 615 c, t dbSNP:371185955
616 616 a, g dbSNP:375482214
617 617 c, t dbSNP:765292870
621 621 c, g, t dbSNP:201822770
630 630 c, t dbSNP:11568511
631 631 a, c, g dbSNP:141478353
635 635 c, t dbSNP:781338157
639 639 c, t dbSNP:745797189
640 640 a, g dbSNP:11568509
645 645 c, g, t dbSNP:113034123
646 646 a, c, g dbSNP:138375296
651 651 a, g dbSNP:372380514
656 656 -, a dbSNP:752780765
659 659 a, g dbSNP:11568510
661 661 a, g dbSNP:768389161
662 662 c, g dbSNP:773811534
670 670 a, g dbSNP:371708747
678 678 c, t dbSNP:771320444
679 679 a, c, g dbSNP:781510670
687 687 c, g, t dbSNP:374839569
688 688 a, g dbSNP:144031153
699 699 c, t dbSNP:768616452
700 700 a, g dbSNP:774674579
705 705 c, g dbSNP:456392
710 710 a, g dbSNP:369071242
720 720 c, t dbSNP:460415
730 730 c, t dbSNP:767803439
732 732 c, t dbSNP:750278877
734 734 c, g dbSNP:760482890
738 738 c, t dbSNP:766356847
749 749 c, t dbSNP:753702919
756 756 a, t dbSNP:754580524
761 761 a, t dbSNP:147297796
764 764 c, t dbSNP:752392368
771 771 c, t dbSNP:11568498
772 772 a, g dbSNP:777364340
779 779 c, t dbSNP:746234716
780 780 a, g dbSNP:11568500
794 794 a, g dbSNP:373520579
795 795 c, g, t dbSNP:143879161
798 798 c, t dbSNP:774318267
799 799 a, g dbSNP:148604311
804 804 a, g dbSNP:376697042
806 806 c, t dbSNP:773512581
807 807 a, c dbSNP:199611329
808 808 g, t dbSNP:369732593
836 836 a, g dbSNP:765091021
840 840 a, g dbSNP:775346954
842 842 c, t dbSNP:201467740
844 844 c, t dbSNP:375988789
845 845 a, c, g dbSNP:143140136
851 851 c, t dbSNP:756882520
861 861 a, g dbSNP:767158433
864 864 a, t dbSNP:146705097
868 868 a, g dbSNP:755317078
884 884 c, t dbSNP:575829481
887 887 a, t dbSNP:541967934
897 897 a, g dbSNP:758587614
900 900 a, g dbSNP:146340469
906 906 a, g dbSNP:747058771
907 907 a, c dbSNP:771085988
909 909 c, g dbSNP:751368946
912 912 c, t dbSNP:776844663
922 922 a, g dbSNP:372778380
925 925 a, c dbSNP:376295543
930 930 c, t dbSNP:370379416
931 931 c, t dbSNP:762856391
933 933 c, g dbSNP:368695578
934 934 a, c, t dbSNP:768533971
945 945 a, g dbSNP:761322837
950 950 c, t dbSNP:767093107
954 954 c, g dbSNP:200918264
956 956 c, t dbSNP:139188733
957 957 a, g dbSNP:144122563
962 962 c, t dbSNP:199535716
963 963 a, g dbSNP:371002294
967 967 c, g dbSNP:758993571
976 976 c, g dbSNP:375525577
977 977 c, t dbSNP:751593840
980 980 c, t dbSNP:757302889
985 985 c, t dbSNP:368056012
1009 1009 c, t dbSNP:11568503
1010 1010 a, g dbSNP:748090484
1017 1017 g, t dbSNP:771527346
1019 1019 c, t dbSNP:769645496
1022 1022 c, g dbSNP:772796676
1039 1039 a, g dbSNP:746475008
1053 1053 c, t dbSNP:770708660
1057 1057 a, c dbSNP:776363804
1058 1058 a, c dbSNP:763467165
1067 1067 a, c dbSNP:201521490
1077 1077 c, t dbSNP:774759030
1082 1082 c, t dbSNP:272893
1090 1090 c, t dbSNP:767872488
1102 1102 c, t dbSNP:750523509
1127 1127 c, t dbSNP:140888297
1128 1128 a, c, t dbSNP:200185233
1130 1130 c, ct, t dbSNP:72552719
1130 1130 g, t dbSNP:770551085
1140 1140 a, g dbSNP:541361427
1167 1167 a, g dbSNP:367863918
1169 1169 c, t dbSNP:768949924
1171 1171 c, t dbSNP:76343123
1172 1172 a, g dbSNP:762018264
1180 1180 a, c, g dbSNP:533127429
1196 1196 a, t dbSNP:567645504
1197 1197 a, g dbSNP:760813238
1209 1209 a, g dbSNP:766418958
1250 1250 a, t dbSNP:752847081
1258 1258 a, g dbSNP:150157940
1264 1264 c, t dbSNP:762870150
1267 1267 a, g dbSNP:575659970
1268 1268 a, g dbSNP:751596030
1279 1279 c, g dbSNP:757282135
1284 1284 c, t dbSNP:781209544
1291 1291 c, t dbSNP:191121049
1339 1339 c, t dbSNP:372962746
1341 1341 a, g dbSNP:779825520
1342 1342 c, t dbSNP:748864045
1343 1343 a, g dbSNP:772540855
1345 1345 a, t dbSNP:778137526
1346 1346 c, t dbSNP:201507559
1347 1347 c, g dbSNP:272879
1348 1348 c, g dbSNP:1050150
1350 1350 a, g dbSNP:148936987
1357 1357 c, t dbSNP:201371114
1358 1358 a, g dbSNP:769756304
1361 1361 a, g dbSNP:775819832
1398 1398 c, g dbSNP:17852518
1404 1404 c, g, t dbSNP:779525039
1412 1412 a, c dbSNP:751405490
1422 1422 c, t dbSNP:761644362
1426 1426 a, g dbSNP:767621862
1427 1427 -, att dbSNP:561053135
1443 1443 a, c dbSNP:377673823
1450 1450 c, t dbSNP:760555279
1456 1456 a, g dbSNP:147032697
1466 1466 a, c dbSNP:753288548
1469 1469 g, t dbSNP:754423266
1473 1473 a, g dbSNP:764924147
1476 1476 a, c dbSNP:529910545
1479 1479 c, t dbSNP:757655893
1481 1481 c, g dbSNP:781386691
1491 1491 a, c dbSNP:746193557
1500 1500 c, t dbSNP:756609362
1508 1508 a, c dbSNP:780552167
1512 1512 c, t dbSNP:370947767
1515 1515 a, g dbSNP:193043140
1517 1517 c, t dbSNP:774755477
1519 1519 c, t dbSNP:748361558
1520 1520 a, t dbSNP:772374561
1522 1522 c, g dbSNP:772915647
1526 1526 c, t dbSNP:760456007
1528 1528 c, g dbSNP:138433908
1530 1530 g, t dbSNP:776614713
1535 1535 a, g dbSNP:759110538
1536 1536 g, t dbSNP:764692894
1537 1537 a, c dbSNP:752394406
1544 1544 c, t dbSNP:200543879
1545 1545 a, c, g dbSNP:199597043
1547 1547 -, ggg dbSNP:373747100
1547 1547 g, t dbSNP:202244640
1550 1550 a, g dbSNP:4646201
1553 1553 g, t dbSNP:200741625
1555 1555 a, g dbSNP:143888295
1556 1556 c, t dbSNP:754367405
1558 1558 c, t dbSNP:530272146
1560 1560 a, c dbSNP:755159592
1562 1562 c, t dbSNP:779159133
1563 1563 a, g dbSNP:201228074
1572 1572 a, t dbSNP:772432542
1577 1577 a, g dbSNP:777989435
1578 1578 c, g dbSNP:12777
1604 1604 a, g dbSNP:770752876
1608 1608 c, t dbSNP:183075585
1609 1609 a, g dbSNP:148341373
1620 1620 a, c dbSNP:775348982
1625 1625 c, t dbSNP:557926964
1636 1636 a, g dbSNP:375225322
1638 1638 c, t dbSNP:141587824
1639 1639 a, g dbSNP:188858026
1640 1640 a, t dbSNP:767034234
1641 1641 c, t dbSNP:566960067
1643 1643 c, t dbSNP:375398349
1657 1657 a, g dbSNP:765906886
1658 1658 c, t dbSNP:753255483
1665 1665 c, t dbSNP:61731074
1670 1670 c, t dbSNP:764214038
1671 1671 a, c dbSNP:57512142
1672 1672 c, t dbSNP:1050152
1674 1674 a, c dbSNP:201450794
1678 1678 -, t dbSNP:776307267
1679 1679 -, t dbSNP:770747109
1685 1685 c, t dbSNP:745677209
1685 1685 -, t dbSNP:72552721
1691 1691 a, t dbSNP:755898461
1707 1707 c, t dbSNP:535181187
1708 1708 a, c, t dbSNP:558087632
1722 1722 a, c dbSNP:749225329
1723 1723 c, g dbSNP:201132879
1732 1732 a, c dbSNP:773940777
1734 1734 a, g dbSNP:146182196
1745 1745 a, c, g dbSNP:771861252
1758 1758 -, a dbSNP:759406751
1773 1773 c, t dbSNP:748994718
1788 1788 a, g dbSNP:754945336
1789 1789 a, g dbSNP:778655134
1790 1790 a, g dbSNP:747748208
1812 1812 c, t dbSNP:116837840
1824 1824 a, c, g dbSNP:11568508
1852 1852 a, g dbSNP:770661459
1853 1853 a, g dbSNP:775956581
1869 1869 a, c dbSNP:763161391
1908 1908 c, t dbSNP:545290223
1914 1914 c, t dbSNP:139332157
1917 1917 a, c, t dbSNP:148289043
1955 1955 c, t dbSNP:560593165
1972 1972 a, g dbSNP:777865006
1988 1988 a, g dbSNP:761506580
1990 1990 c, t dbSNP:532865558
2040 2040 a, c dbSNP:143426016
2090 2090 c, t dbSNP:371985838
2099 2099 c, t dbSNP:771241244
2123 2123 c, t dbSNP:112601131

Target ORF information:

RefSeq Version NM_003059
Organism Homo sapiens (human)
Definition Homo sapiens solute carrier family 22 (organic cation/zwitterion transporter), member 4 (SLC22A4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003059

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCGGGACT ACGACGAGGT GATCGCCTTC CTGGGCGAGT GGGGGCCCTT CCAGCGCCTC 
ATCTTCTTCC TGCTCAGCGC CAGCATCATC CCCAATGGCT TCAATGGTAT GTCAGTCGTG
TTCCTGGCGG GGACCCCGGA GCACCGCTGT CGAGTGCCGG ACGCCGCGAA CCTGAGCAGC
GCCTGGCGCA ACAACAGTGT CCCGCTGCGG CTGCGGGACG GCCGCGAGGT GCCCCACAGC
TGCAGCCGCT ACCGGCTCGC CACCATCGCC AACTTCTCGG CGCTCGGGCT GGAGCCGGGG
CGCGACGTGG ACCTGGGGCA GCTGGAGCAG GAGAGCTGCC TGGATGGCTG GGAGTTCAGC
CAGGACGTCT ACCTGTCCAC CGTCGTGACC GAGTGGAATC TGGTGTGTGA GGACAACTGG
AAGGTGCCCC TCACCACCTC CCTGTTCTTC GTAGGCGTGC TCCTCGGCTC CTTCGTGTCC
GGGCAGCTGT CAGACAGGTT TGGCAGGAAG AACGTTCTCT TCGCAACCAT GGCTGTACAG
ACTGGCTTCA GCTTCCTGCA GATTTTCTCC ATCAGCTGGG AGATGTTCAC TGTGTTATTT
GTCATCGTGG GCATGGGCCA GATCTCCAAC TATGTGGTAG CCTTCATACT AGGAACAGAA
ATTCTTGGCA AGTCAGTTCG TATTATATTC TCTACATTAG GAGTGTGCAC ATTTTTTGCA
GTTGGCTATA TGCTGCTGCC ACTGTTTGCT TACTTCATCA GAGACTGGCG GATGCTGCTG
CTGGCGCTGA CGGTGCCGGG AGTGCTGTGT GTCCCGCTGT GGTGGTTCAT TCCTGAATCT
CCCCGATGGC TGATATCCCA GAGAAGATTT AGAGAGGCTG AAGATATCAT CCAAAAAGCT
GCAAAAATGA ACAACATAGC TGTACCAGCA GTGATATTTG ATTCTGTGGA GGAGCTAAAT
CCCCTGAAGC AGCAGAAAGC TTTCATTCTG GACCTGTTCA GGACTCGGAA TATTGCCATA
ATGACCATTA TGTCTTTGCT GCTATGGATG CTGACCTCAG TGGGTTACTT TGCTCTGTCT
CTGGATGCTC CTAATTTACA TGGAGATGCC TACCTGAACT GTTTCCTCTC TGCCTTGATT
GAAATTCCAG CTTACATTAC AGCCTGGCTG CTATTGCGAA CCCTGCCCAG GCGTTATATC
ATAGCTGCAG TACTGTTCTG GGGAGGAGGT GTGCTTCTCT TCATTCAACT GGTACCTGTG
GATTATTACT TCTTATCCAT TGGTCTGGTC ATGCTGGGAA AATTTGGGAT CACCTCTGCT
TTCTCCATGC TGTATGTCTT CACTGCTGAG CTCTACCCAA CCCTGGTCAG GAACATGGCG
GTGGGGGTCA CATCCACGGC CTCCAGAGTG GGCAGCATCA TTGCCCCCTA CTTTGTTTAC
CTCGGTGCTT ACAACAGAAT GCTGCCCTAC ATCGTCATGG GTAGTCTGAC TGTCCTGATT
GGAATCCTCA CCCTTTTTTT CCCTGAAAGT TTGGGAATGA CTCTTCCAGA AACCTTAGAG
CAGATGCAGA AAGTGAAATG GTTCAGATCT GGGAAAAAAA CAAGAGACTC AATGGAGACA
GAAGAAAATC CCAAGGTTCT AATAACTGCA TTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.