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SMARCE1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol SMARCE1
Entrez Gene ID 6605
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
Synonyms BAF57
General protein information
Preferred Names
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
Names
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
BRG1-associated factor 57
chromatin remodeling complex BRG1-associated factor 57
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q21.2

Summary The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

603111


mRNA and Protein(s)

mRNA Protein Name
NM_003079 NP_003070 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1



Homo sapiens (human) SMARCE1 NP_003070.3
Pan troglodytes (chimpanzee) SMARCE1 XP_511478.2
Macaca mulatta (Rhesus monkey) SMARCE1 XP_001099623.2
Canis lupus familiaris (dog) SMARCE1 XP_851010.1
Bos taurus (cattle) SMARCE1 NP_001092586.1
Mus musculus (house mouse) Smarce1 NP_065643.1
Rattus norvegicus (Norway rat) Smarce1 NP_001020164.1
Gallus gallus (chicken) SMARCE1 NP_001006335.2
Danio rerio (zebrafish) smarce1 NP_958455.2
Caenorhabditis elegans swsn-3 NP_497613.2
Xenopus (Silurana) tropicalis (western clawed frog) smarce1 NP_001005436.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SMARCE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMARCE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu24945
NM_003079 Homo sapiens SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (SMARCE1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu24945
Clone ID Related Accession (Same CDS sequence) NM_003079
Accession Version NM_003079.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1236bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ707908.1, AK095047.1 and BC063700.1. This sequence is a reference standard in the RefSeqGene project. On Mar 30, 2004 this sequence version replaced gi:21264354. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC069196.1, AK095047.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962346 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCAAAAA GACCATCTTA TGCCCCACCT CCCACCCCAG CTCCTGCAAC ACAAATGCCC 
AGCACACCAG GGTTTGTGGG ATACAATCCA TACAGTCATC TCGCCTACAA CAACTACAGG
CTGGGAGGGA ACCCGGGCAC CAACAGCCGG GTCACGGCAT CCTCTGGTAT CACGATTCCA
AAACCCCCAA AGCCACCAGA TAAGCCGCTG ATGCCCTACA TGAGGTACAG CAGAAAGGTC
TGGGACCAAG TAAAGGCTTC CAACCCTGAC CTAAAGTTGT GGGAGATTGG CAAGATTATT
GGTGGCATGT GGCGAGATCT CACTGATGAA GAAAAACAAG AATATTTAAA CGAATACGAA
GCAGAAAAGA TAGAGTACAA TGAATCTATG AAGGCCTATC ATAATTCCCC CGCGTACCTT
GCTTACATAA ATGCAAAAAG TCGTGCAGAA GCTGCTTTAG AGGAAGAAAG TCGACAGAGA
CAATCTCGCA TGGAGAAAGG AGAACCGTAC ATGAGCATTC AGCCTGCTGA AGATCCAGAT
GATTATGATG ATGGCTTTTC AATGAAGCAT ACAGCCACCG CCCGTTTCCA GAGAAACCAC
CGCCTCATCA GTGAAATTCT TAGTGAGAGT GTGGTGCCAG ACGTTCGGTC AGTTGTCACA
ACAGCTAGAA TGCAGGTCCT CAAACGGCAG GTCCAGTCCT TAATGGTTCA TCAGCGAAAA
CTAGAAGCTG AACTTCTTCA AATAGAGGAA CGACACCAGG AGAAGAAGAG GAAATTCCTG
GAAAGCACAG ATTCATTTAA CAATGAACTT AAAAGGTTGT GCGGTCTGAA AGTAGAAGTG
GATATGGAGA AAATTGCAGC TGAGATTGCA CAGGCAGAGG AACAGGCCCG CAAAAGGCAG
GAGGAAAGGG AGAAGGAGGC CGCAGAGCAA GCTGAGCGCA GTCAGAGCAG CATCGTTCCT
GAGGAAGAAC AAGCAGCTAA CAAAGGCGAG GAGAAGAAAG ACGACGAGAA CATTCCGATG
GAGACAGAGG AGACACACCT TGAAGAAACA ACAGAGAGCC AACAGAATGG TGAAGAAGGC
ACGTCTACTC CTGAGGACAA GGAGAGTGGG CAGGAGGGGG TCGACAGTAT GGCAGAGGAA
GGAACCAGTG ATAGTAACAC TGGCTCGGAG AGCAACAGTG CAACAGTGGA GGAGCCACCA
ACAGATCCCA TACCAGAAGA TGAGAAAAAA GAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003070.3
CDS125..1360
Misc Feature(1)113..115(+)
Misc Feature(2)332..517(+)
Misc Feature(3)332..493(+)
Misc Feature(4)773..1024(+)
Misc Feature(5)917..919(+)
Exon (1)1..79
Gene:SMARCE1
Gene Synonym:
Exon (2)80..131
Gene:SMARCE1
Gene Synonym:
Exon (3)132..175
Gene:SMARCE1
Gene Synonym:
Exon (4)176..280
Gene:SMARCE1
Gene Synonym:
Exon (5)281..361
Gene:SMARCE1
Gene Synonym:
Exon (6)362..493
Gene:SMARCE1
Gene Synonym:
Exon (7)494..665
Gene:SMARCE1
Gene Synonym:
Exon (8)666..838
Gene:SMARCE1
Gene Synonym:
Exon (9)839..940
Gene:SMARCE1
Gene Synonym:
Exon (10)941..1151
Gene:SMARCE1
Gene Synonym:
Exon (11)1152..2425
Gene:SMARCE1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_003079
Organism Homo sapiens (human)
Definition Homo sapiens SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (SMARCE1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003079

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCAAAAA GACCATCTTA TGCCCCACCT CCCACCCCAG CTCCTGCAAC ACAAATGCCC 
AGCACACCAG GGTTTGTGGG ATACAATCCA TACAGTCATC TCGCCTACAA CAACTACAGG
CTGGGAGGGA ACCCGGGCAC CAACAGCCGG GTCACGGCAT CCTCTGGTAT CACGATTCCA
AAACCCCCAA AGCCACCAGA TAAGCCGCTG ATGCCCTACA TGAGGTACAG CAGAAAGGTC
TGGGACCAAG TAAAGGCTTC CAACCCTGAC CTAAAGTTGT GGGAGATTGG CAAGATTATT
GGTGGCATGT GGCGAGATCT CACTGATGAA GAAAAACAAG AATATTTAAA CGAATACGAA
GCAGAAAAGA TAGAGTACAA TGAATCTATG AAGGCCTATC ATAATTCCCC CGCGTACCTT
GCTTACATAA ATGCAAAAAG TCGTGCAGAA GCTGCTTTAG AGGAAGAAAG TCGACAGAGA
CAATCTCGCA TGGAGAAAGG AGAACCGTAC ATGAGCATTC AGCCTGCTGA AGATCCAGAT
GATTATGATG ATGGCTTTTC AATGAAGCAT ACAGCCACCG CCCGTTTCCA GAGAAACCAC
CGCCTCATCA GTGAAATTCT TAGTGAGAGT GTGGTGCCAG ACGTTCGGTC AGTTGTCACA
ACAGCTAGAA TGCAGGTCCT CAAACGGCAG GTCCAGTCCT TAATGGTTCA TCAGCGAAAA
CTAGAAGCTG AACTTCTTCA AATAGAGGAA CGACACCAGG AGAAGAAGAG GAAATTCCTG
GAAAGCACAG ATTCATTTAA CAATGAACTT AAAAGGTTGT GCGGTCTGAA AGTAGAAGTG
GATATGGAGA AAATTGCAGC TGAGATTGCA CAGGCAGAGG AACAGGCCCG CAAAAGGCAG
GAGGAAAGGG AGAAGGAGGC CGCAGAGCAA GCTGAGCGCA GTCAGAGCAG CATCGTTCCT
GAGGAAGAAC AAGCAGCTAA CAAAGGCGAG GAGAAGAAAG ACGACGAGAA CATTCCGATG
GAGACAGAGG AGACACACCT TGAAGAAACA ACAGAGAGCC AACAGAATGG TGAAGAAGGC
ACGTCTACTC CTGAGGACAA GGAGAGTGGG CAGGAGGGGG TCGACAGTAT GGCAGAGGAA
GGAACCAGTG ATAGTAACAC TGGCTCGGAG AGCAACAGTG CAACAGTGGA GGAGCCACCA
ACAGATCCCA TACCAGAAGA TGAGAAAAAA GAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Aberrant BAF57 signaling facilitates prometastatic phenotypes
Clin. Cancer Res. 19 (10), 2657-2667 (2013)
Balasubramaniam S, Comstock CE, Ertel A, Jeong KW, Stallcup MR, Addya S, McCue PA, Ostrander WF Jr, Augello MA and Knudsen KE.


book

Loss-of-function mutations in SMARCE1 cause an inherited disorder of multiple spinal meningiomas
Nat. Genet. 45 (3), 295-298 (2013)
Smith MJ, O'Sullivan J, Bhaskar SS, Hadfield KD, Poke G, Caird J, Sharif S, Eccles D, Fitzpatrick D, Rawluk D, du Plessis D, Newman WG and Evans DG.


book

Prognostic significance of BAF57 expression in patients with endometrial carcinoma
Histol. Histopathol. 27 (5), 593-599 (2012)
Kagami S, Kurita T, Kawagoe T, Toki N, Matsuura Y, Hachisuga T, Matsuyama A, Hashimoto H, Izumi H and Kohno K.


book

Identification and characterization of novel potentially oncogenic mutations in the human BAF57 gene in a breast cancer patient
Breast Cancer Res. Treat. 128 (3), 891-898 (2011)
Villaronga MA, Lopez-Mateo I, Markert L, Espinosa E, Fresno Vara JA and Belandia B.


book

Ubiquitin-dependent and ubiquitin-independent control of subunit stoichiometry in the SWI/SNF complex
J. Biol. Chem. 285 (46), 35665-35674 (2010)
Keppler BR and Archer TK.


book

Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits
Mol. Cell 3 (2), 247-253 (1999)
Phelan ML, Sif S, Narlikar GJ and Kingston RE.


book

Architectural DNA binding by a high-mobility-group/kinesin-like subunit in mammalian SWI/SNF-related complexes
Proc. Natl. Acad. Sci. U.S.A. 95 (2), 492-498 (1998)
Wang W, Chi T, Xue Y, Zhou S, Kuo A and Crabtree GR.


book

Purification and biochemical heterogeneity of the mammalian SWI-SNF complex
EMBO J. 15 (19), 5370-5382 (1996)
Wang W, Cote J, Xue Y, Zhou S, Khavari PA, Biggar SR, Muchardt C, Kalpana GV, Goff SP, Yaniv M, Workman JL and Crabtree GR.


book

Diversity and specialization of mammalian SWI/SNF complexes
Genes Dev. 10 (17), 2117-2130 (1996)
Wang W, Xue Y, Zhou S, Kuo A, Cairns BR and Crabtree GR.


book

Coffin-Siris Syndrome
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Schrier Vergano,S., Santen,G., Wieczorek,D., Wollnik,B., Matsumoto,N. and Deardorff,M.A.


 
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