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SNRPA cDNA ORF clone, Homo sapiens (human)

Gene Symbol SNRPA
Entrez Gene ID 6626
Full Name small nuclear ribonucleoprotein polypeptide A
Synonyms Mud1, U1-A, U1A
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19q13.1

Summary The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation. [provided by RefSeq, Oct 2010]. lac of sum
Disorder MIM:

182285


mRNA and Protein(s)

mRNA Protein Name
NM_004596 NP_004587 U1 small nuclear ribonucleoprotein A



Homo sapiens (human) SNRPA NP_004587.1
Pan troglodytes (chimpanzee) SNRPA XP_512674.2
Macaca mulatta (Rhesus monkey) SNRPA XP_001098504.1
Canis lupus familiaris (dog) SNRPA XP_533663.2
Bos taurus (cattle) SNRPA NP_001039504.1
Rattus norvegicus (Norway rat) Snrpa NP_001008304.1
Danio rerio (zebrafish) snrpa NP_955965.1
Drosophila melanogaster (fruit fly) snf NP_511045.1
Caenorhabditis elegans rnp-2 NP_500504.1
Arabidopsis thaliana (thale cress) U1A NP_182280.1
Xenopus (Silurana) tropicalis (western clawed frog) snrpa NP_001016784.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SNRPA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SNRPA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu15951
NM_004596 Homo sapiens small nuclear ribonucleoprotein polypeptide A (SNRPA), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
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30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu15951
Clone ID Related Accession (Same CDS sequence) NM_004596
Accession Version NM_004596.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 849bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product U1 small nuclear ribonucleoprotein A
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN286572.1, BC000405.2, AC020945.6 and BQ002918.1. On Apr 28, 2010 this sequence version replaced gi:39725715. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000405.2, X06347.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCAGTTC CCGAGACCCG CCCTAACCAC ACTATTTATA TCAACAACCT CAATGAGAAG 
ATCAAGAAGG ATGAGCTAAA AAAGTCCCTG TACGCCATCT TCTCCCAGTT TGGCCAGATC
CTGGATATCC TGGTATCACG GAGCCTGAAG ATGAGGGGCC AGGCCTTTGT CATCTTCAAG
GAGGTCAGCA GCGCCACCAA CGCCCTGCGC TCCATGCAGG GTTTCCCTTT CTATGACAAA
CCTATGCGTA TCCAGTATGC CAAGACCGAC TCAGATATCA TTGCCAAGAT GAAAGGCACC
TTCGTGGAGC GGGACCGCAA GCGGGAGAAG AGGAAGCCCA AGAGCCAGGA GACCCCGGCC
ACCAAGAAGG CTGTGCAAGG CGGGGGAGCC ACCCCCGTGG TGGGGGCTGT CCAGGGGCCT
GTCCCGGGCA TGCCGCCGAT GACTCAGGCG CCCCGCATTA TGCACCACAT GCCGGGCCAG
CCGCCCTACA TGCCGCCCCC TGGTATGATC CCCCCGCCAG GCCTTGCACC TGGCCAGATC
CCACCAGGGG CCATGCCCCC GCAGCAGCTT ATGCCAGGAC AGATGCCCCC TGCCCAGCCT
CTTTCTGAGA ATCCACCGAA TCACATCTTG TTCCTCACCA ACCTGCCAGA GGAGACCAAC
GAGCTCATGC TGTCCATGCT TTTCAATCAG TTCCCTGGCT TCAAGGAGGT CCGTCTGGTA
CCCGGGCGGC ATGACATCGC CTTCGTGGAG TTTGACAATG AGGTACAGGC AGGGGCAGCT
CGCGATGCCC TGCAGGGCTT TAAGATCACG CAGAACAACG CCATGAAGAT CTCCTTTGCC
AAGAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004587.1
CDS556..1404
Misc Feature(1)508..510(+)
Misc Feature(2)559..561(+)
Misc Feature(3)574..840(+)
Misc Feature(4)586..807(+)
Misc Feature(5)592..831(+)
Misc Feature(6)733..735(+)
Misc Feature(7)946..948(+)
Misc Feature(8)1162..1401(+)
Misc Feature(9)1183..1362(+)
Exon (1)1..628
Gene:SNRPA
Gene Synonym:
Exon (2)629..801
Gene:SNRPA
Gene Synonym:
Exon (3)802..981
Gene:SNRPA
Gene Synonym:
Exon (4)982..1155
Gene:SNRPA
Gene Synonym:
Exon (5)1156..1244
Gene:SNRPA
Gene Synonym:
Exon (6)1245..1629
Gene:SNRPA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_004596
Organism Homo sapiens (human)
Definition Homo sapiens small nuclear ribonucleoprotein polypeptide A (SNRPA), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004596

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCAGTTC CCGAGACCCG CCCTAACCAC ACTATTTATA TCAACAACCT CAATGAGAAG 
ATCAAGAAGG ATGAGCTAAA AAAGTCCCTG TACGCCATCT TCTCCCAGTT TGGCCAGATC
CTGGATATCC TGGTATCACG GAGCCTGAAG ATGAGGGGCC AGGCCTTTGT CATCTTCAAG
GAGGTCAGCA GCGCCACCAA CGCCCTGCGC TCCATGCAGG GTTTCCCTTT CTATGACAAA
CCTATGCGTA TCCAGTATGC CAAGACCGAC TCAGATATCA TTGCCAAGAT GAAAGGCACC
TTCGTGGAGC GGGACCGCAA GCGGGAGAAG AGGAAGCCCA AGAGCCAGGA GACCCCGGCC
ACCAAGAAGG CTGTGCAAGG CGGGGGAGCC ACCCCCGTGG TGGGGGCTGT CCAGGGGCCT
GTCCCGGGCA TGCCGCCGAT GACTCAGGCG CCCCGCATTA TGCACCACAT GCCGGGCCAG
CCGCCCTACA TGCCGCCCCC TGGTATGATC CCCCCGCCAG GCCTTGCACC TGGCCAGATC
CCACCAGGGG CCATGCCCCC GCAGCAGCTT ATGCCAGGAC AGATGCCCCC TGCCCAGCCT
CTTTCTGAGA ATCCACCGAA TCACATCTTG TTCCTCACCA ACCTGCCAGA GGAGACCAAC
GAGCTCATGC TGTCCATGCT TTTCAATCAG TTCCCTGGCT TCAAGGAGGT CCGTCTGGTA
CCCGGGCGGC ATGACATCGC CTTCGTGGAG TTTGACAATG AGGTACAGGC AGGGGCAGCT
CGCGATGCCC TGCAGGGCTT TAAGATCACG CAGAACAACG CCATGAAGAT CTCCTTTGCC
AAGAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

U1A regulates 3' processing of the survival motor neuron mRNA
J. Biol. Chem. 289 (6), 3703-3712 (2014)
Workman E, Veith A and Battle DJ.


book

U1A protein-stem loop 2 RNA recognition: prediction of structural differences from protein mutations
Biopolymers 95 (9), 591-606 (2011)
Kormos BL, Pieniazek SN, Beveridge DL and Baranger AM.


book

The small nuclear ribonucleoprotein U1A interacts with NS5 from yellow fever virus
Arch. Virol. 156 (6), 931-938 (2011)
Bronzoni RV, Madrid MC, Duarte DV, Pellegrini VO, Pacca CC, Carmo AC, Zanelli CF, Valentini SR, Santacruz-Perez C, Barbosa JA, Lutz CS, Rahal P and Nogueira ML.


book

Multistep kinetics of the U1A-SL2 RNA complex dissociation
J. Mol. Biol. 408 (5), 896-908 (2011)
Anunciado D, Dhar A, Gruebele M and Baranger AM.


book

Identification of molecular contacts between the U1 A small nuclear ribonucleoprotein and U1 RNA
EMBO J. 10 (11), 3447-3456 (1991)
Jessen TH, Oubridge C, Teo CH, Pritchard C and Nagai K.


book

Structure, chromosomal localization and evolutionary conservation of the gene encoding human U1 snRNP-specific A protein
Gene 102 (2), 189-196 (1991)
Nelissen RL, Sillekens PT, Beijer RP, Geurts van Kessel AH and van Venrooij WJ.


book

RNA-binding domain of the A protein component of the U1 small nuclear ribonucleoprotein analyzed by NMR spectroscopy is structurally similar to ribosomal proteins
Proc. Natl. Acad. Sci. U.S.A. 88 (6), 2495-2499 (1991)
Hoffman DW, Query CC, Golden BL, White SW and Keene JD.


book

Quantitative determination that one of two potential RNA-binding domains of the A protein component of the U1 small nuclear ribonucleoprotein complex binds with high affinity to stem-loop II of U1 RNA
Proc. Natl. Acad. Sci. U.S.A. 87 (16), 6393-6397 (1990)
Lutz-Freyermuth C, Query CC and Keene JD.


book

Assignment of seven genes to distinct intervals on the midportion of human chromosome 19q surrounding the myotonic dystrophy gene region
Cytogenet. Cell Genet. 54 (1-2), 15-19 (1990)
Schonk D, van Dijk P, Riegmann P, Trapman J, Holm C, Willcocks TC, Sillekens P, van Venrooij W, Wimmer E and Geurts van Kessel A.


book

cDNA cloning of the human U1 snRNA-associated A protein: extensive homology between U1 and U2 snRNP-specific proteins
EMBO J. 6 (12), 3841-3848 (1987)
Sillekens PT, Habets WJ, Beijer RP and van Venrooij WJ.


 
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