×

SPTA1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol SPTA1
Entrez Gene ID 6708
Full Name spectrin, alpha, erythrocytic 1
Synonyms EL2, HPP, HS3, SPH3, SPTA
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q21

Summary Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is a tetramer made up of alpha-beta dimers linked in a head-to-head arrangement. This gene is one member of a family of alpha-spectrin genes. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms weaker tetramer interactions than non-erythrocytic alpha spectrin, which may increase the plasma membrane elasticity and deformability of red blood cells. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis type 2, pyropoikilocytosis, and spherocytic hemolytic anemia. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

182860

Disorder Html: Elliptocytosis-2, 130600 (3); Pyropoikilocytosis, 266140 (3);

mRNA and Protein(s)

mRNA Protein Name
XM_011509916 XP_011508218 spectrin alpha chain, erythrocytic 1 isoform X1
XM_011509917 XP_011508219 spectrin alpha chain, erythrocytic 1 isoform X2
XM_011509918 XP_011508220 spectrin alpha chain, erythrocytic 1 isoform X3
XM_011509919 XP_011508221 spectrin alpha chain, erythrocytic 1 isoform X4
NM_003126 NP_003117 spectrin alpha chain, erythrocytic 1



Homo sapiens (human) SPTA1 NP_003117.2
Pan troglodytes (chimpanzee) SPTA1 XP_001169940.1
Macaca mulatta (Rhesus monkey) SPTA1 XP_001117115.1
Canis lupus familiaris (dog) SPTA1 XP_545733.3
Bos taurus (cattle) SPTA1 NP_001193517.1
Mus musculus (house mouse) Spta1 NP_035595.2
Rattus norvegicus (Norway rat) Spta1 NP_001011908.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPTA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu28487 XM_011509916 PREDICTED: Homo sapiens spectrin, alpha, erythrocytic 1 (SPTA1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1301.30
$1859.00
OHu60716 XM_011509917 PREDICTED: Homo sapiens spectrin, alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1301.30
$1859.00
OHu60717 XM_011509918 PREDICTED: Homo sapiens spectrin, alpha, erythrocytic 1 (SPTA1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1091.30
$1559.00
OHu60718 XM_011509919 PREDICTED: Homo sapiens spectrin, alpha, erythrocytic 1 (SPTA1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $951.30
$1359.00
OHu28487 NM_003126 Homo sapiens spectrin, alpha, erythrocytic 1 (SPTA1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1301.30
$1859.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu28487
Clone ID Related Accession (Same CDS sequence) NM_003126 , XM_011509916
Accession Version XM_011509916.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 7260bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product spectrin alpha chain, erythrocytic 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA
TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT
CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA
AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG
GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG
CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC
CAGGCCAAGG ACATCAAAGG TGTGAGTGAA GAGACTCTAA AGGAATTTAG CACAATCTAT
AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG
AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG
AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT
GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC
TTCCAAGCCC TGGCAGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT
ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT
CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTACTT TGGCAACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508218.1
CDS69..7328
Misc Feature(1)129..542(+)
Misc Feature(2)222..239(+)
Misc Feature(3)543..1178(+)
Misc Feature(4)855..872(+)
Misc Feature(5)864..1496(+)
Misc Feature(6)1173..1190(+)
Misc Feature(7)1497..2129(+)
Misc Feature(8)1809..1826(+)
Misc Feature(9)2130..2759(+)
Misc Feature(10)2442..2459(+)
Misc Feature(11)2766..3617(+)
Misc Feature(12)3012..3167(+)
Misc Feature(13)3024..3158(+)
Misc Feature(14)3309..3326(+)
Misc Feature(15)3624..4253(+)
Misc Feature(16)3930..3947(+)
Misc Feature(17)4254..4886(+)
Misc Feature(18)4563..4580(+)
Misc Feature(19)4890..5525(+)
Misc Feature(20)5202..5219(+)
Misc Feature(21)5211..5846(+)
Misc Feature(22)5520..5537(+)
Misc Feature(23)5856..6512(+)
Misc Feature(24)6171..6188(+)
Misc Feature(25)6207..6848(+)
Misc Feature(26)6510..6527(+)
Misc Feature(27)6891..7097(+)
Misc Feature(28)6918..7082(+)
Misc Feature(29)7116..7322(+)
Translation

Target ORF information:

RefSeq Version XM_011509916
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens spectrin, alpha, erythrocytic 1 (SPTA1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509916

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA
TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT
CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA
AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG
GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG
CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC
CAGGCCAAGG ACATCAAAGG TGTGAGTGAA GAGACTCTAA AGGAATTTAG CACAATCTAT
AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG
AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG
AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT
GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC
TTCCAAGCCC TGGCAGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT
ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT
CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTACTT TGGCAACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60716
Clone ID Related Accession (Same CDS sequence) XM_011509917
Accession Version XM_011509917.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 7242bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product spectrin alpha chain, erythrocytic 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA
TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG ATCATTGCTC
AAAGAAACAG GAACTCTGGA ATCTCAGCTG GAAGCAAATA AAAGAAAACA GAAGGAGATC
CAGGCGATGA AGCGTCAACT AACCAAGATT GTGGACCTGG GGGACAACTT GGAAGACGCT
CTGATCCTTG ATATCAAATA CAGCACCATT GGATTGGCTC AGCAGTGGGA CCAGCTCTAC
CAGCTTGGGT TGCGGATGCA ACACAACCTG GAGCAACAGA TCCAGGCCAA GGACATCAAA
GGTGTGAGTG AAGAGACTCT AAAGGAATTT AGCACAATCT ATAAACACTT TGATGAGAAT
TTGACAGGGC GCCTGACTCA CAAAGAGTTC CGGTCCTGCC TGAGAGGACT CAATTACTAC
TTGCCCATGG TGGAGGAGGA TGAACATGAG CCCAAGTTTG AGAAGTTCCT GGATGCTGTG
GATCCAGGGA GGAAGGGCTA TGTCTCACTG GAGGACTATA CTGCTTTCCT GATTGACAAG
GAGTCAGAAA ACATCAAGTC CAGTGATGAA ATAGAGAATG CCTTCCAAGC CCTGGCAGAG
GGCAAGTCAT ATATTACCAA AGAAGACATG AAGCAGGCCC TTACCCCAGA GCAAGTGTCA
TTCTGTGCCA CACATATGCA GCAATATATG GACCCACGGG GTCGAAGCCA TCTCTCTGGC
TATGACTACG TTGGCTTCAC CAATTCCTAC TTTGGCAACT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508219.1
CDS110..7351
Misc Feature(1)170..583(+)
Misc Feature(2)263..280(+)
Misc Feature(3)584..1219(+)
Misc Feature(4)896..913(+)
Misc Feature(5)905..1537(+)
Misc Feature(6)1214..1231(+)
Misc Feature(7)1538..2170(+)
Misc Feature(8)1850..1867(+)
Misc Feature(9)2171..2800(+)
Misc Feature(10)2483..2500(+)
Misc Feature(11)2807..3658(+)
Misc Feature(12)3053..3208(+)
Misc Feature(13)3065..3199(+)
Misc Feature(14)3350..3367(+)
Misc Feature(15)3665..4294(+)
Misc Feature(16)3971..3988(+)
Misc Feature(17)4295..4927(+)
Misc Feature(18)4604..4621(+)
Misc Feature(19)4931..5566(+)
Misc Feature(20)5243..5260(+)
Misc Feature(21)5252..5887(+)
Misc Feature(22)5561..5578(+)
Misc Feature(23)5897..6553(+)
Misc Feature(24)6212..6229(+)
Misc Feature(25)6248..6871(+)
Misc Feature(26)6551..6568(+)
Misc Feature(27)6914..7120(+)
Misc Feature(28)6941..7105(+)
Misc Feature(29)7139..7345(+)
Translation

Target ORF information:

RefSeq Version XM_011509917
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens spectrin, alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509917

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA
TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG ATCATTGCTC
AAAGAAACAG GAACTCTGGA ATCTCAGCTG GAAGCAAATA AAAGAAAACA GAAGGAGATC
CAGGCGATGA AGCGTCAACT AACCAAGATT GTGGACCTGG GGGACAACTT GGAAGACGCT
CTGATCCTTG ATATCAAATA CAGCACCATT GGATTGGCTC AGCAGTGGGA CCAGCTCTAC
CAGCTTGGGT TGCGGATGCA ACACAACCTG GAGCAACAGA TCCAGGCCAA GGACATCAAA
GGTGTGAGTG AAGAGACTCT AAAGGAATTT AGCACAATCT ATAAACACTT TGATGAGAAT
TTGACAGGGC GCCTGACTCA CAAAGAGTTC CGGTCCTGCC TGAGAGGACT CAATTACTAC
TTGCCCATGG TGGAGGAGGA TGAACATGAG CCCAAGTTTG AGAAGTTCCT GGATGCTGTG
GATCCAGGGA GGAAGGGCTA TGTCTCACTG GAGGACTATA CTGCTTTCCT GATTGACAAG
GAGTCAGAAA ACATCAAGTC CAGTGATGAA ATAGAGAATG CCTTCCAAGC CCTGGCAGAG
GGCAAGTCAT ATATTACCAA AGAAGACATG AAGCAGGCCC TTACCCCAGA GCAAGTGTCA
TTCTGTGCCA CACATATGCA GCAATATATG GACCCACGGG GTCGAAGCCA TCTCTCTGGC
TATGACTACG TTGGCTTCAC CAATTCCTAC TTTGGCAACT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60717
Clone ID Related Accession (Same CDS sequence) XM_011509918
Accession Version XM_011509918.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6126bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product spectrin alpha chain, erythrocytic 1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
GTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508220.1
CDS112..6237
Misc Feature(1)172..585(+)
Misc Feature(2)265..282(+)
Misc Feature(3)586..1221(+)
Misc Feature(4)898..915(+)
Misc Feature(5)907..1539(+)
Misc Feature(6)1216..1233(+)
Misc Feature(7)1540..2172(+)
Misc Feature(8)1852..1869(+)
Misc Feature(9)2173..2802(+)
Misc Feature(10)2485..2502(+)
Misc Feature(11)2815..>2985(+)
Misc Feature(12)3055..3210(+)
Misc Feature(13)3067..3201(+)
Misc Feature(14)3352..3369(+)
Misc Feature(15)3355..3648(+)
Misc Feature(16)3667..4296(+)
Misc Feature(17)3973..3990(+)
Misc Feature(18)4297..4929(+)
Misc Feature(19)4606..4623(+)
Misc Feature(20)4933..5568(+)
Misc Feature(21)5245..5262(+)
Misc Feature(22)5254..5889(+)
Misc Feature(23)5563..5580(+)
Misc Feature(24)5899..>6231(+)
Misc Feature(25)6214..6231(+)
Translation

Target ORF information:

RefSeq Version XM_011509918
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens spectrin, alpha, erythrocytic 1 (SPTA1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509918

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
GTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60718
Clone ID Related Accession (Same CDS sequence) XM_011509919
Accession Version XM_011509919.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5088bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product spectrin alpha chain, erythrocytic 1 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
GGAGAAGTTG ATACGAGTGA GCTCCCAGGA CTATGGGAGA GATCTTCAGG GGGTTCAGAA
CTTGCTGAAG AAGCACAAAC GCCTAGAGGG GGAGCTGGTG GCCCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508221.1
CDS115..5202
Misc Feature(1)175..588(+)
Misc Feature(2)268..285(+)
Misc Feature(3)589..1224(+)
Misc Feature(4)901..918(+)
Misc Feature(5)910..1542(+)
Misc Feature(6)1219..1236(+)
Misc Feature(7)1543..2175(+)
Misc Feature(8)1855..1872(+)
Misc Feature(9)2176..2805(+)
Misc Feature(10)2488..2505(+)
Misc Feature(11)2812..3663(+)
Misc Feature(12)3058..3213(+)
Misc Feature(13)3070..3204(+)
Misc Feature(14)3355..3372(+)
Misc Feature(15)3670..4299(+)
Misc Feature(16)3976..3993(+)
Misc Feature(17)4300..4932(+)
Misc Feature(18)4609..4626(+)
Misc Feature(19)4936..>5109(+)
Translation

Target ORF information:

RefSeq Version XM_011509919
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens spectrin, alpha, erythrocytic 1 (SPTA1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509919

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
GGAGAAGTTG ATACGAGTGA GCTCCCAGGA CTATGGGAGA GATCTTCAGG GGGTTCAGAA
CTTGCTGAAG AAGCACAAAC GCCTAGAGGG GGAGCTGGTG GCCCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28487
Clone ID Related Accession (Same CDS sequence) NM_003126 , XM_011509916
Accession Version NM_003126.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 7260bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product spectrin alpha chain, erythrocytic 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL353894.14, M61877.1, CD616351.1 and BU665064.1. This sequence is a reference standard in the RefSeqGene project. On Sep 22, 2006 this sequence version replaced gi:4507188. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M61877.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA
TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT
CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA
AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG
GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG
CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC
CAGGCCAAGG ACATCAAAGG TGTGAGTGAA GAGACTCTAA AGGAATTTAG CACAATCTAT
AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG
AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG
AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT
GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC
TTCCAAGCCC TGGCAGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT
ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT
CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTACTT TGGCAACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003117.2
CDS200..7459
Misc Feature(1)1..1(+)
Misc Feature(2)47..49(+)
Misc Feature(3)254..352(+)
Misc Feature(4)260..673(+)
Misc Feature(5)353..370(+)
Misc Feature(6)356..667(+)
Misc Feature(7)671..985(+)
Misc Feature(8)674..1309(+)
Misc Feature(9)986..1003(+)
Misc Feature(10)989..1303(+)
Misc Feature(11)995..1627(+)
Misc Feature(12)1304..1321(+)
Misc Feature(13)1307..1621(+)
Misc Feature(14)1625..1939(+)
Misc Feature(15)1628..2260(+)
Misc Feature(16)1940..1957(+)
Misc Feature(17)1943..2254(+)
Misc Feature(18)2258..2572(+)
Misc Feature(19)2261..2890(+)
Misc Feature(20)2573..2590(+)
Misc Feature(21)2576..2890(+)
Misc Feature(22)2894..3103(+)
Misc Feature(23)2897..3748(+)
Misc Feature(24)3143..3298(+)
Misc Feature(25)3155..3289(+)
Misc Feature(26)3440..3457(+)
Misc Feature(27)3443..3742(+)
Misc Feature(28)3746..4060(+)
Misc Feature(29)3755..4384(+)
Misc Feature(30)4061..4078(+)
Misc Feature(31)4064..4378(+)
Misc Feature(32)4382..4693(+)
Misc Feature(33)4385..5017(+)
Misc Feature(34)4694..4711(+)
Misc Feature(35)4697..5014(+)
Misc Feature(36)5018..5332(+)
Misc Feature(37)5021..5656(+)
Misc Feature(38)5327..5329(+)
Misc Feature(39)5333..5350(+)
Misc Feature(40)5336..5650(+)
Misc Feature(41)5342..5977(+)
Misc Feature(42)5651..5668(+)
Misc Feature(43)5654..5977(+)
Misc Feature(44)5981..6298(+)
Misc Feature(45)5987..6643(+)
Misc Feature(46)6302..6319(+)
Misc Feature(47)6326..6640(+)
Misc Feature(48)6338..6979(+)
Misc Feature(49)6641..6658(+)
Misc Feature(50)6668..6973(+)
Misc Feature(51)7022..7228(+)
Misc Feature(52)7049..7213(+)
Misc Feature(53)7247..7453(+)
Exon (1)1..223
Gene:SPTA1
Gene Synonym:
Exon (2)224..463
Gene:SPTA1
Gene Synonym:
Exon (3)464..589
Gene:SPTA1
Gene Synonym:
Exon (4)590..730
Gene:SPTA1
Gene Synonym:
Exon (5)731..877
Gene:SPTA1
Gene Synonym:
Exon (6)878..1011
Gene:SPTA1
Gene Synonym:
Exon (7)1012..1156
Gene:SPTA1
Gene Synonym:
Exon (8)1157..1311
Gene:SPTA1
Gene Synonym:
Exon (9)1312..1447
Gene:SPTA1
Gene Synonym:
Exon (10)1448..1549
Gene:SPTA1
Gene Synonym:
Exon (11)1550..1687
Gene:SPTA1
Gene Synonym:
Exon (12)1688..1798
Gene:SPTA1
Gene Synonym:
Exon (13)1799..1876
Gene:SPTA1
Gene Synonym:
Exon (14)1877..2032
Gene:SPTA1
Gene Synonym:
Exon (15)2033..2237
Gene:SPTA1
Gene Synonym:
Exon (16)2238..2419
Gene:SPTA1
Gene Synonym:
Exon (17)2420..2663
Gene:SPTA1
Gene Synonym:
Exon (18)2664..2786
Gene:SPTA1
Gene Synonym:
Exon (19)2787..3004
Gene:SPTA1
Gene Synonym:
Exon (20)3005..3097
Gene:SPTA1
Gene Synonym:
Exon (21)3098..3235
Gene:SPTA1
Gene Synonym:
Exon (22)3236..3387
Gene:SPTA1
Gene Synonym:
Exon (23)3388..3574
Gene:SPTA1
Gene Synonym:
Exon (24)3575..3676
Gene:SPTA1
Gene Synonym:
Exon (25)3677..3768
Gene:SPTA1
Gene Synonym:
Exon (26)3769..3913
Gene:SPTA1
Gene Synonym:
Exon (27)3914..4095
Gene:SPTA1
Gene Synonym:
Exon (28)4096..4195
Gene:SPTA1
Gene Synonym:
Exon (29)4196..4393
Gene:SPTA1
Gene Synonym:
Exon (30)4394..4537
Gene:SPTA1
Gene Synonym:
Exon (31)4538..4641
Gene:SPTA1
Gene Synonym:
Exon (32)4642..4804
Gene:SPTA1
Gene Synonym:
Exon (33)4805..4936
Gene:SPTA1
Gene Synonym:
Exon (34)4937..5074
Gene:SPTA1
Gene Synonym:
Exon (35)5075..5179
Gene:SPTA1
Gene Synonym:
Exon (36)5180..5388
Gene:SPTA1
Gene Synonym:
Exon (37)5389..5509
Gene:SPTA1
Gene Synonym:
Exon (38)5510..5631
Gene:SPTA1
Gene Synonym:
Exon (39)5632..5764
Gene:SPTA1
Gene Synonym:
Exon (40)5765..5863
Gene:SPTA1
Gene Synonym:
Exon (41)5864..6032
Gene:SPTA1
Gene Synonym:
Exon (42)6033..6109
Gene:SPTA1
Gene Synonym:
Exon (43)6110..6319
Gene:SPTA1
Gene Synonym:
Exon (44)6320..6616
Gene:SPTA1
Gene Synonym:
Exon (45)6617..6729
Gene:SPTA1
Gene Synonym:
Exon (46)6730..6747
Gene:SPTA1
Gene Synonym:
Exon (47)6748..6799
Gene:SPTA1
Gene Synonym:
Exon (48)6800..6987
Gene:SPTA1
Gene Synonym:
Exon (49)6988..7041
Gene:SPTA1
Gene Synonym:
Exon (50)7042..7188
Gene:SPTA1
Gene Synonym:
Exon (51)7189..7333
Gene:SPTA1
Gene Synonym:
Exon (52)7334..8017
Gene:SPTA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_003126
Organism Homo sapiens (human)
Definition Homo sapiens spectrin, alpha, erythrocytic 1 (SPTA1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003126

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
7021
7081
7141
7201
ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA
TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT
CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA
AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG
GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG
CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC
CAGGCCAAGG ACATCAAAGG TGTGAGTGAA GAGACTCTAA AGGAATTTAG CACAATCTAT
AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG
AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG
AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT
GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC
TTCCAAGCCC TGGCAGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT
ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT
CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTACTT TGGCAACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
*
*
*
*