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BRCA1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol BRCA1
Entrez Gene ID 672
Full Name breast cancer 1, early onset
Synonyms BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53
General protein information
Preferred Names
breast cancer type 1 susceptibility protein
Names
breast cancer type 1 susceptibility protein
RING finger protein 53
BRCA1/BRCA2-containing complex, subunit 1
protein phosphatase 1, regulatory subunit 53
breast and ovarian cancer susceptibility protein 1
breast and ovarian cancer sususceptibility protein 1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q21

Summary This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]. lac of sum
Disorder MIM:

113705

Disorder Html: {Breast-ovarian cancer, familial, 1}, 604370 (3); {Pancreatic cancer,

mRNA and Protein(s)

mRNA Protein Name
NM_007297 NP_009228 breast cancer type 1 susceptibility protein isoform 3
NM_007299 NP_009230 breast cancer type 1 susceptibility protein isoform 5
NM_007294 NP_009225 breast cancer type 1 susceptibility protein isoform 1
NM_007300 NP_009231 breast cancer type 1 susceptibility protein isoform 2
NM_007298 NP_009229 breast cancer type 1 susceptibility protein isoform 4



Homo sapiens (human) BRCA1 NP_009231.2
Pan troglodytes (chimpanzee) BRCA1 NP_001038958.1
Macaca mulatta (Rhesus monkey) BRCA1 NP_001108421.1
Canis lupus familiaris (dog) BRCA1 NP_001013434.1
Bos taurus (cattle) BRCA1 NP_848668.1
Mus musculus (house mouse) Brca1 NP_033894.3
Rattus norvegicus (Norway rat) Brca1 NP_036646.1
Gallus gallus (chicken) BRCA1 NP_989500.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRCA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRCA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu16595
NM_007297 Homo sapiens breast cancer 1, early onset (BRCA1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$659.50-$923.30
$1319.00
OHu16598 NM_007299 Homo sapiens breast cancer 1, early onset (BRCA1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $496.30
$709.00
OHu18572
NM_007294 Homo sapiens breast cancer 1, early onset (BRCA1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$659.50-$923.30
$1319.00
OHu23229 NM_007300 Homo sapiens breast cancer 1, early onset (BRCA1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $265.30
$379.00
OHu16419 NM_007298 Homo sapiens breast cancer 1, early onset (BRCA1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu16595
Clone ID Related Accession (Same CDS sequence) NM_007297
Accession Version NM_007297.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5451bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 03-AUG-2015
Organism Homo sapiens (human)
Product breast cancer type 1 susceptibility protein isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC072418.1, U14680.1, BU617173.1 and BU679389.1. On May 16, 2009 this sequence version replaced gi:63252874. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and uses a downstream translational start codon, compared to variant 1. The encoded isoform (3) is shorter at the N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
ATGCTGAAAC TTCTCAACCA GAAGAAAGGG CCTTCACAGT GTCCTTTATG TAAGAATGAT 
ATAACCAAAA GGAGCCTACA AGAAAGTACG AGATTTAGTC AACTTGTTGA AGAGCTATTG
AAAATCATTT GTGCTTTTCA GCTTGACACA GGTTTGGAGT ATGCAAACAG CTATAATTTT
GCAAAAAAGG AAAATAACTC TCCTGAACAT CTAAAAGATG AAGTTTCTAT CATCCAAAGT
ATGGGCTACA GAAACCGTGC CAAAAGACTT CTACAGAGTG AACCCGAAAA TCCTTCCTTG
CAGGAAACCA GTCTCAGTGT CCAACTCTCT AACCTTGGAA CTGTGAGAAC TCTGAGGACA
AAGCAGCGGA TACAACCTCA AAAGACGTCT GTCTACATTG AATTGGGATC TGATTCTTCT
GAAGATACCG TTAATAAGGC AACTTATTGC AGTGTGGGAG ATCAAGAATT GTTACAAATC
ACCCCTCAAG GAACCAGGGA TGAAATCAGT TTGGATTCTG CAAAAAAGGC TGCTTGTGAA
TTTTCTGAGA CGGATGTAAC AAATACTGAA CATCATCAAC CCAGTAATAA TGATTTGAAC
ACCACTGAGA AGCGTGCAGC TGAGAGGCAT CCAGAAAAGT ATCAGGGTAG TTCTGTTTCA
AACTTGCATG TGGAGCCATG TGGCACAAAT ACTCATGCCA GCTCATTACA GCATGAGAAC
AGCAGTTTAT TACTCACTAA AGACAGAATG AATGTAGAAA AGGCTGAATT CTGTAATAAA
AGCAAACAGC CTGGCTTAGC AAGGAGCCAA CATAACAGAT GGGCTGGAAG TAAGGAAACA
TGTAATGATA GGCGGACTCC CAGCACAGAA AAAAAGGTAG ATCTGAATGC TGATCCCCTG
TGTGAGAGAA AAGAATGGAA TAAGCAGAAA CTGCCATGCT CAGAGAATCC TAGAGATACT
GAAGATGTTC CTTGGATAAC ACTAAATAGC AGCATTCAGA AAGTTAATGA GTGGTTTTCC
AGAAGTGATG AACTGTTAGG TTCTGATGAC TCACATGATG GGGAGTCTGA ATCAAATGCC
AAAGTAGCTG ATGTATTGGA CGTTCTAAAT GAGGTAGATG AATATTCTGG TTCTTCAGAG
AAAATAGACT TACTGGCCAG TGATCCTCAT GAGGCTTTAA TATGTAAAAG TGAAAGAGTT
CACTCCAAAT CAGTAGAGAG TAATATTGAA GACAAAATAT TTGGGAAAAC CTATCGGAAG
AAGGCAAGCC TCCCCAACTT AAGCCATGTA ACTGAAAATC TAATTATAGG AGCATTTGTT
ACTGAGCCAC AGATAATACA AGAGCGTCCC CTCACAAATA AATTAAAGCG TAAAAGGAGA
CCTACATCAG GCCTTCATCC TGAGGATTTT ATCAAGAAAG CAGATTTGGC AGTTCAAAAG
ACTCCTGAAA TGATAAATCA GGGAACTAAC CAAACGGAGC AGAATGGTCA AGTGATGAAT
ATTACTAATA GTGGTCATGA GAATAAAACA AAAGGTGATT CTATTCAGAA TGAGAAAAAT
CCTAACCCAA TAGAATCACT CGAAAAAGAA TCTGCTTTCA AAACGAAAGC TGAACCTATA
AGCAGCAGTA TAAGCAATAT GGAACTCGAA TTAAATATCC ACAATTCAAA AGCACCTAAA
AAGAATAGGC TGAGGAGGAA GTCTTCTACC AGGCATATTC ATGCGCTTGA ACTAGTAGTC
AGTAGAAATC TAAGCCCACC TAATTGTACT GAATTGCAAA TTGATAGTTG TTCTAGCAGT
GAAGAGATAA AGAAAAAAAA GTACAACCAA ATGCCAGTCA GGCACAGCAG AAACCTACAA
CTCATGGAAG GTAAAGAACC TGCAACTGGA GCCAAGAAGA GTAACAAGCC AAATGAACAG
ACAAGTAAAA GACATGACAG CGATACTTTC CCAGAGCTGA AGTTAACAAA TGCACCTGGT
TCTTTTACTA AGTGTTCAAA TACCAGTGAA CTTAAAGAAT TTGTCAATCC TAGCCTTCCA
AGAGAAGAAA AAGAAGAGAA ACTAGAAACA GTTAAAGTGT CTAATAATGC TGAAGACCCC
AAAGATCTCA TGTTAAGTGG AGAAAGGGTT TTGCAAACTG AAAGATCTGT AGAGAGTAGC
AGTATTTCAT TGGTACCTGG TACTGATTAT GGCACTCAGG AAAGTATCTC GTTACTGGAA
GTTAGCACTC TAGGGAAGGC AAAAACAGAA CCAAATAAAT GTGTGAGTCA GTGTGCAGCA
TTTGAAAACC CCAAGGGACT AATTCATGGT TGTTCCAAAG ATAATAGAAA TGACACAGAA
GGCTTTAAGT ATCCATTGGG ACATGAAGTT AACCACAGTC GGGAAACAAG CATAGAAATG
GAAGAAAGTG AACTTGATGC TCAGTATTTG CAGAATACAT TCAAGGTTTC AAAGCGCCAG
TCATTTGCTC CGTTTTCAAA TCCAGGAAAT GCAGAAGAGG AATGTGCAAC ATTCTCTGCC
CACTCTGGGT CCTTAAAGAA ACAAAGTCCA AAAGTCACTT TTGAATGTGA ACAAAAGGAA
GAAAATCAAG GAAAGAATGA GTCTAATATC AAGCCTGTAC AGACAGTTAA TATCACTGCA
GGCTTTCCTG TGGTTGGTCA GAAAGATAAG CCAGTTGATA ATGCCAAATG TAGTATCAAA
GGAGGCTCTA GGTTTTGTCT ATCATCTCAG TTCAGAGGCA ACGAAACTGG ACTCATTACT
CCAAATAAAC ATGGACTTTT ACAAAACCCA TATCGTATAC CACCACTTTT TCCCATCAAG
TCATTTGTTA AAACTAAATG TAAGAAAAAT CTGCTAGAGG AAAACTTTGA GGAACATTCA
ATGTCACCTG AAAGAGAAAT GGGAAATGAG AACATTCCAA GTACAGTGAG CACAATTAGC
CGTAATAACA TTAGAGAAAA TGTTTTTAAA GAAGCCAGCT CAAGCAATAT TAATGAAGTA
GGTTCCAGTA CTAATGAAGT GGGCTCCAGT ATTAATGAAA TAGGTTCCAG TGATGAAAAC
ATTCAAGCAG AACTAGGTAG AAACAGAGGG CCAAAATTGA ATGCTATGCT TAGATTAGGG
GTTTTGCAAC CTGAGGTCTA TAAACAAAGT CTTCCTGGAA GTAATTGTAA GCATCCTGAA
ATAAAAAAGC AAGAATATGA AGAAGTAGTT CAGACTGTTA ATACAGATTT CTCTCCATAT
CTGATTTCAG ATAACTTAGA ACAGCCTATG GGAAGTAGTC ATGCATCTCA GGTTTGTTCT
GAGACACCTG ATGACCTGTT AGATGATGGT GAAATAAAGG AAGATACTAG TTTTGCTGAA
AATGACATTA AGGAAAGTTC TGCTGTTTTT AGCAAAAGCG TCCAGAAAGG AGAGCTTAGC
AGGAGTCCTA GCCCTTTCAC CCATACACAT TTGGCTCAGG GTTACCGAAG AGGGGCCAAG
AAATTAGAGT CCTCAGAAGA GAACTTATCT AGTGAGGATG AAGAGCTTCC CTGCTTCCAA
CACTTGTTAT TTGGTAAAGT AAACAATATA CCTTCTCAGT CTACTAGGCA TAGCACCGTT
GCTACCGAGT GTCTGTCTAA GAACACAGAG GAGAATTTAT TATCATTGAA GAATAGCTTA
AATGACTGCA GTAACCAGGT AATATTGGCA AAGGCATCTC AGGAACATCA CCTTAGTGAG
GAAACAAAAT GTTCTGCTAG CTTGTTTTCT TCACAGTGCA GTGAATTGGA AGACTTGACT
GCAAATACAA ACACCCAGGA TCCTTTCTTG ATTGGTTCTT CCAAACAAAT GAGGCATCAG
TCTGAAAGCC AGGGAGTTGG TCTGAGTGAC AAGGAATTGG TTTCAGATGA TGAAGAAAGA
GGAACGGGCT TGGAAGAAAA TAATCAAGAA GAGCAAAGCA TGGATTCAAA CTTAGGTGAA
GCAGCATCTG GGTGTGAGAG TGAAACAAGC GTCTCTGAAG ACTGCTCAGG GCTATCCTCT
CAGAGTGACA TTTTAACCAC TCAGCAGAGG GATACCATGC AACATAACCT GATAAAGCTC
CAGCAGGAAA TGGCTGAACT AGAAGCTGTG TTAGAACAGC ATGGGAGCCA GCCTTCTAAC
AGCTACCCTT CCATCATAAG TGACTCTTCT GCCCTTGAGG ACCTGCGAAA TCCAGAACAA
AGCACATCAG AAAAAGCAGT ATTAACTTCA CAGAAAAGTA GTGAATACCC TATAAGCCAG
AATCCAGAAG GCCTTTCTGC TGACAAGTTT GAGGTGTCTG CAGATAGTTC TACCAGTAAA
AATAAAGAAC CAGGAGTGGA AAGGTCATCC CCTTCTAAAT GCCCATCATT AGATGATAGG
TGGTACATGC ACAGTTGCTC TGGGAGTCTT CAGAATAGAA ACTACCCATC TCAAGAGGAG
CTCATTAAGG TTGTTGATGT GGAGGAGCAA CAGCTGGAAG AGTCTGGGCC ACACGATTTG
ACGGAAACAT CTTACTTGCC AAGGCAAGAT CTAGAGGGAA CCCCTTACCT GGAATCTGGA
ATCAGCCTCT TCTCTGATGA CCCTGAATCT GATCCTTCTG AAGACAGAGC CCCAGAGTCA
GCTCGTGTTG GCAACATACC ATCTTCAACC TCTGCATTGA AAGTTCCCCA ATTGAAAGTT
GCAGAATCTG CCCAGAGTCC AGCTGCTGCT CATACTACTG ATACTGCTGG GTATAATGCA
ATGGAAGAAA GTGTGAGCAG GGAGAAGCCA GAATTGACAG CTTCAACAGA AAGGGTCAAC
AAAAGAATGT CCATGGTGGT GTCTGGCCTG ACCCCAGAAG AATTTATGCT CGTGTACAAG
TTTGCCAGAA AACACCACAT CACTTTAACT AATCTAATTA CTGAAGAGAC TACTCATGTT
GTTATGAAAA CAGATGCTGA GTTTGTGTGT GAACGGACAC TGAAATATTT TCTAGGAATT
GCGGGAGGAA AATGGGTAGT TAGCTATTTC TGGGTGACCC AGTCTATTAA AGAAAGAAAA
ATGCTGAATG AGCATGATTT TGAAGTCAGA GGAGATGTGG TCAATGGAAG AAACCACCAA
GGTCCAAAGC GAGCAAGAGA ATCCCAGGAC AGAAAGATCT TCAGGGGGCT AGAAATCTGT
TGCTATGGGC CCTTCACCAA CATGCCCACA GATCAACTGG AATGGATGGT ACAGCTGTGT
GGTGCTTCTG TGGTGAAGGA GCTTTCATCA TTCACCCTTG GCACAGGTGT CCACCCAATT
GTGGTTGTGC AGCCAGATGC CTGGACAGAG GACAATGGCT TCCATGCAAT TGGGCAGATG
TGTGAGGCAC CTGTGGTGAC CCGAGAGTGG GTGTTGGACA GTGTAGCACT CTACCAGTGC
CAGGAGCTGG ACACCTACCT GATACCCCAG ATCCCCCACA GCCACTACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_009228.2
CDS282..5732
Misc Feature(1)61..61(+)
Misc Feature(2)273..275(+)
Misc Feature(3)1170..1661(+)
Misc Feature(4)2082..3074(+)
Misc Feature(5)5088..5312(+)
Misc Feature(6)5139..5165(+)
Misc Feature(7)5292..5306(+)
Misc Feature(8)5427..5666(+)
Misc Feature(9)5472..5510(+)
Misc Feature(10)5649..5663(+)
Exon (1)1..175
Gene:BRCA1
Gene Synonym:
Exon (2)176..274
Gene:BRCA1
Gene Synonym:
Exon (3)275..352
Gene:BRCA1
Gene Synonym:
Exon (4)353..441
Gene:BRCA1
Gene Synonym:
Exon (5)442..581
Gene:BRCA1
Gene Synonym:
Exon (6)582..687
Gene:BRCA1
Gene Synonym:
Exon (7)688..733
Gene:BRCA1
Gene Synonym:
Exon (8)734..810
Gene:BRCA1
Gene Synonym:
Exon (9)811..4236
Gene:BRCA1
Gene Synonym:
Exon (10)4237..4325
Gene:BRCA1
Gene Synonym:
Exon (11)4326..4497
Gene:BRCA1
Gene Synonym:
Exon (12)4498..4624
Gene:BRCA1
Gene Synonym:
Exon (13)4625..4815
Gene:BRCA1
Gene Synonym:
Exon (14)4816..5126
Gene:BRCA1
Gene Synonym:
Exon (15)5127..5214
Gene:BRCA1
Gene Synonym:
Exon (16)5215..5292
Gene:BRCA1
Gene Synonym:
Exon (17)5293..5333
Gene:BRCA1
Gene Synonym:
Exon (18)5334..5417
Gene:BRCA1
Gene Synonym:
Exon (19)5418..5472
Gene:BRCA1
Gene Synonym:
Exon (20)5473..5546
Gene:BRCA1
Gene Synonym:
Exon (21)5547..5607
Gene:BRCA1
Gene Synonym:
Exon (22)5608..7115
Gene:BRCA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_007297
Organism Homo sapiens (human)
Definition Homo sapiens breast cancer 1, early onset (BRCA1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_007297

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
ATGCTGAAAC TTCTCAACCA GAAGAAAGGG CCTTCACAGT GTCCTTTATG TAAGAATGAT 
ATAACCAAAA GGAGCCTACA AGAAAGTACG AGATTTAGTC AACTTGTTGA AGAGCTATTG
AAAATCATTT GTGCTTTTCA GCTTGACACA GGTTTGGAGT ATGCAAACAG CTATAATTTT
GCAAAAAAGG AAAATAACTC TCCTGAACAT CTAAAAGATG AAGTTTCTAT CATCCAAAGT
ATGGGCTACA GAAACCGTGC CAAAAGACTT CTACAGAGTG AACCCGAAAA TCCTTCCTTG
CAGGAAACCA GTCTCAGTGT CCAACTCTCT AACCTTGGAA CTGTGAGAAC TCTGAGGACA
AAGCAGCGGA TACAACCTCA AAAGACGTCT GTCTACATTG AATTGGGATC TGATTCTTCT
GAAGATACCG TTAATAAGGC AACTTATTGC AGTGTGGGAG ATCAAGAATT GTTACAAATC
ACCCCTCAAG GAACCAGGGA TGAAATCAGT TTGGATTCTG CAAAAAAGGC TGCTTGTGAA
TTTTCTGAGA CGGATGTAAC AAATACTGAA CATCATCAAC CCAGTAATAA TGATTTGAAC
ACCACTGAGA AGCGTGCAGC TGAGAGGCAT CCAGAAAAGT ATCAGGGTAG TTCTGTTTCA
AACTTGCATG TGGAGCCATG TGGCACAAAT ACTCATGCCA GCTCATTACA GCATGAGAAC
AGCAGTTTAT TACTCACTAA AGACAGAATG AATGTAGAAA AGGCTGAATT CTGTAATAAA
AGCAAACAGC CTGGCTTAGC AAGGAGCCAA CATAACAGAT GGGCTGGAAG TAAGGAAACA
TGTAATGATA GGCGGACTCC CAGCACAGAA AAAAAGGTAG ATCTGAATGC TGATCCCCTG
TGTGAGAGAA AAGAATGGAA TAAGCAGAAA CTGCCATGCT CAGAGAATCC TAGAGATACT
GAAGATGTTC CTTGGATAAC ACTAAATAGC AGCATTCAGA AAGTTAATGA GTGGTTTTCC
AGAAGTGATG AACTGTTAGG TTCTGATGAC TCACATGATG GGGAGTCTGA ATCAAATGCC
AAAGTAGCTG ATGTATTGGA CGTTCTAAAT GAGGTAGATG AATATTCTGG TTCTTCAGAG
AAAATAGACT TACTGGCCAG TGATCCTCAT GAGGCTTTAA TATGTAAAAG TGAAAGAGTT
CACTCCAAAT CAGTAGAGAG TAATATTGAA GACAAAATAT TTGGGAAAAC CTATCGGAAG
AAGGCAAGCC TCCCCAACTT AAGCCATGTA ACTGAAAATC TAATTATAGG AGCATTTGTT
ACTGAGCCAC AGATAATACA AGAGCGTCCC CTCACAAATA AATTAAAGCG TAAAAGGAGA
CCTACATCAG GCCTTCATCC TGAGGATTTT ATCAAGAAAG CAGATTTGGC AGTTCAAAAG
ACTCCTGAAA TGATAAATCA GGGAACTAAC CAAACGGAGC AGAATGGTCA AGTGATGAAT
ATTACTAATA GTGGTCATGA GAATAAAACA AAAGGTGATT CTATTCAGAA TGAGAAAAAT
CCTAACCCAA TAGAATCACT CGAAAAAGAA TCTGCTTTCA AAACGAAAGC TGAACCTATA
AGCAGCAGTA TAAGCAATAT GGAACTCGAA TTAAATATCC ACAATTCAAA AGCACCTAAA
AAGAATAGGC TGAGGAGGAA GTCTTCTACC AGGCATATTC ATGCGCTTGA ACTAGTAGTC
AGTAGAAATC TAAGCCCACC TAATTGTACT GAATTGCAAA TTGATAGTTG TTCTAGCAGT
GAAGAGATAA AGAAAAAAAA GTACAACCAA ATGCCAGTCA GGCACAGCAG AAACCTACAA
CTCATGGAAG GTAAAGAACC TGCAACTGGA GCCAAGAAGA GTAACAAGCC AAATGAACAG
ACAAGTAAAA GACATGACAG CGATACTTTC CCAGAGCTGA AGTTAACAAA TGCACCTGGT
TCTTTTACTA AGTGTTCAAA TACCAGTGAA CTTAAAGAAT TTGTCAATCC TAGCCTTCCA
AGAGAAGAAA AAGAAGAGAA ACTAGAAACA GTTAAAGTGT CTAATAATGC TGAAGACCCC
AAAGATCTCA TGTTAAGTGG AGAAAGGGTT TTGCAAACTG AAAGATCTGT AGAGAGTAGC
AGTATTTCAT TGGTACCTGG TACTGATTAT GGCACTCAGG AAAGTATCTC GTTACTGGAA
GTTAGCACTC TAGGGAAGGC AAAAACAGAA CCAAATAAAT GTGTGAGTCA GTGTGCAGCA
TTTGAAAACC CCAAGGGACT AATTCATGGT TGTTCCAAAG ATAATAGAAA TGACACAGAA
GGCTTTAAGT ATCCATTGGG ACATGAAGTT AACCACAGTC GGGAAACAAG CATAGAAATG
GAAGAAAGTG AACTTGATGC TCAGTATTTG CAGAATACAT TCAAGGTTTC AAAGCGCCAG
TCATTTGCTC CGTTTTCAAA TCCAGGAAAT GCAGAAGAGG AATGTGCAAC ATTCTCTGCC
CACTCTGGGT CCTTAAAGAA ACAAAGTCCA AAAGTCACTT TTGAATGTGA ACAAAAGGAA
GAAAATCAAG GAAAGAATGA GTCTAATATC AAGCCTGTAC AGACAGTTAA TATCACTGCA
GGCTTTCCTG TGGTTGGTCA GAAAGATAAG CCAGTTGATA ATGCCAAATG TAGTATCAAA
GGAGGCTCTA GGTTTTGTCT ATCATCTCAG TTCAGAGGCA ACGAAACTGG ACTCATTACT
CCAAATAAAC ATGGACTTTT ACAAAACCCA TATCGTATAC CACCACTTTT TCCCATCAAG
TCATTTGTTA AAACTAAATG TAAGAAAAAT CTGCTAGAGG AAAACTTTGA GGAACATTCA
ATGTCACCTG AAAGAGAAAT GGGAAATGAG AACATTCCAA GTACAGTGAG CACAATTAGC
CGTAATAACA TTAGAGAAAA TGTTTTTAAA GAAGCCAGCT CAAGCAATAT TAATGAAGTA
GGTTCCAGTA CTAATGAAGT GGGCTCCAGT ATTAATGAAA TAGGTTCCAG TGATGAAAAC
ATTCAAGCAG AACTAGGTAG AAACAGAGGG CCAAAATTGA ATGCTATGCT TAGATTAGGG
GTTTTGCAAC CTGAGGTCTA TAAACAAAGT CTTCCTGGAA GTAATTGTAA GCATCCTGAA
ATAAAAAAGC AAGAATATGA AGAAGTAGTT CAGACTGTTA ATACAGATTT CTCTCCATAT
CTGATTTCAG ATAACTTAGA ACAGCCTATG GGAAGTAGTC ATGCATCTCA GGTTTGTTCT
GAGACACCTG ATGACCTGTT AGATGATGGT GAAATAAAGG AAGATACTAG TTTTGCTGAA
AATGACATTA AGGAAAGTTC TGCTGTTTTT AGCAAAAGCG TCCAGAAAGG AGAGCTTAGC
AGGAGTCCTA GCCCTTTCAC CCATACACAT TTGGCTCAGG GTTACCGAAG AGGGGCCAAG
AAATTAGAGT CCTCAGAAGA GAACTTATCT AGTGAGGATG AAGAGCTTCC CTGCTTCCAA
CACTTGTTAT TTGGTAAAGT AAACAATATA CCTTCTCAGT CTACTAGGCA TAGCACCGTT
GCTACCGAGT GTCTGTCTAA GAACACAGAG GAGAATTTAT TATCATTGAA GAATAGCTTA
AATGACTGCA GTAACCAGGT AATATTGGCA AAGGCATCTC AGGAACATCA CCTTAGTGAG
GAAACAAAAT GTTCTGCTAG CTTGTTTTCT TCACAGTGCA GTGAATTGGA AGACTTGACT
GCAAATACAA ACACCCAGGA TCCTTTCTTG ATTGGTTCTT CCAAACAAAT GAGGCATCAG
TCTGAAAGCC AGGGAGTTGG TCTGAGTGAC AAGGAATTGG TTTCAGATGA TGAAGAAAGA
GGAACGGGCT TGGAAGAAAA TAATCAAGAA GAGCAAAGCA TGGATTCAAA CTTAGGTGAA
GCAGCATCTG GGTGTGAGAG TGAAACAAGC GTCTCTGAAG ACTGCTCAGG GCTATCCTCT
CAGAGTGACA TTTTAACCAC TCAGCAGAGG GATACCATGC AACATAACCT GATAAAGCTC
CAGCAGGAAA TGGCTGAACT AGAAGCTGTG TTAGAACAGC ATGGGAGCCA GCCTTCTAAC
AGCTACCCTT CCATCATAAG TGACTCTTCT GCCCTTGAGG ACCTGCGAAA TCCAGAACAA
AGCACATCAG AAAAAGCAGT ATTAACTTCA CAGAAAAGTA GTGAATACCC TATAAGCCAG
AATCCAGAAG GCCTTTCTGC TGACAAGTTT GAGGTGTCTG CAGATAGTTC TACCAGTAAA
AATAAAGAAC CAGGAGTGGA AAGGTCATCC CCTTCTAAAT GCCCATCATT AGATGATAGG
TGGTACATGC ACAGTTGCTC TGGGAGTCTT CAGAATAGAA ACTACCCATC TCAAGAGGAG
CTCATTAAGG TTGTTGATGT GGAGGAGCAA CAGCTGGAAG AGTCTGGGCC ACACGATTTG
ACGGAAACAT CTTACTTGCC AAGGCAAGAT CTAGAGGGAA CCCCTTACCT GGAATCTGGA
ATCAGCCTCT TCTCTGATGA CCCTGAATCT GATCCTTCTG AAGACAGAGC CCCAGAGTCA
GCTCGTGTTG GCAACATACC ATCTTCAACC TCTGCATTGA AAGTTCCCCA ATTGAAAGTT
GCAGAATCTG CCCAGAGTCC AGCTGCTGCT CATACTACTG ATACTGCTGG GTATAATGCA
ATGGAAGAAA GTGTGAGCAG GGAGAAGCCA GAATTGACAG CTTCAACAGA AAGGGTCAAC
AAAAGAATGT CCATGGTGGT GTCTGGCCTG ACCCCAGAAG AATTTATGCT CGTGTACAAG
TTTGCCAGAA AACACCACAT CACTTTAACT AATCTAATTA CTGAAGAGAC TACTCATGTT
GTTATGAAAA CAGATGCTGA GTTTGTGTGT GAACGGACAC TGAAATATTT TCTAGGAATT
GCGGGAGGAA AATGGGTAGT TAGCTATTTC TGGGTGACCC AGTCTATTAA AGAAAGAAAA
ATGCTGAATG AGCATGATTT TGAAGTCAGA GGAGATGTGG TCAATGGAAG AAACCACCAA
GGTCCAAAGC GAGCAAGAGA ATCCCAGGAC AGAAAGATCT TCAGGGGGCT AGAAATCTGT
TGCTATGGGC CCTTCACCAA CATGCCCACA GATCAACTGG AATGGATGGT ACAGCTGTGT
GGTGCTTCTG TGGTGAAGGA GCTTTCATCA TTCACCCTTG GCACAGGTGT CCACCCAATT
GTGGTTGTGC AGCCAGATGC CTGGACAGAG GACAATGGCT TCCATGCAAT TGGGCAGATG
TGTGAGGCAC CTGTGGTGAC CCGAGAGTGG GTGTTGGACA GTGTAGCACT CTACCAGTGC
CAGGAGCTGG ACACCTACCT GATACCCCAG ATCCCCCACA GCCACTACTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16598
Clone ID Related Accession (Same CDS sequence) NM_007299
Accession Version NM_007299.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2100bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 03-AUG-2015
Organism Homo sapiens (human)
Product breast cancer type 1 susceptibility protein isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC072418.1, BU617173.1 and BU679389.1. On May 16, 2009 this sequence version replaced gi:63252876. Transcript Variant: This variant (5) differs in the 5' UTR, uses two alternate in-frame splice sites in the central coding region, and lacks an alternate exon in the 3' coding region that results in a frameshift, compared to variant 1. The encoded isoform (5) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC072418.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGATTTAT CTGCTCTTCG CGTTGAAGAA GTACAAAATG TCATTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCCATCTG TCTGGAGTTG ATCAAGGAAC CTGTCTCCAC AAAGTGTGAC
CACATATTTT GCAAATTTTG CATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAG
TGTCCTTTAT GTAAGAATGA TATAACCAAA AGGAGCCTAC AAGAAAGTAC GAGATTTAGT
CAACTTGTTG AAGAGCTATT GAAAATCATT TGTGCTTTTC AGCTTGACAC AGGTTTGGAG
TATGCAAACA GCTATAATTT TGCAAAAAAG GAAAATAACT CTCCTGAACA TCTAAAAGAT
GAAGTTTCTA TCATCCAAAG TATGGGCTAC AGAAACCGTG CCAAAAGACT TCTACAGAGT
GAACCCGAAA ATCCTTCCTT GCAGGAAACC AGTCTCAGTG TCCAACTCTC TAACCTTGGA
ACTGTGAGAA CTCTGAGGAC AAAGCAGCGG ATACAACCTC AAAAGACGTC TGTCTACATT
GAATTGGGAT CTGATTCTTC TGAAGATACC GTTAATAAGG CAACTTATTG CAGTGTGGGA
GATCAAGAAT TGTTACAAAT CACCCCTCAA GGAACCAGGG ATGAAATCAG TTTGGATTCT
GCAAAAAAGG CTGCTTGTGA ATTTTCTGAG ACGGATGTAA CAAATACTGA ACATCATCAA
CCCAGTAATA ATGATTTGAA CACCACTGAG AAGCGTGCAG CTGAGAGGCA TCCAGAAAAG
TATCAGGGTG AAGCAGCATC TGGGTGTGAG AGTGAAACAA GCGTCTCTGA AGACTGCTCA
GGGCTATCCT CTCAGAGTGA CATTTTAACC ACTCAGCAGA GGGATACCAT GCAACATAAC
CTGATAAAGC TCCAGCAGGA AATGGCTGAA CTAGAAGCTG TGTTAGAACA GCATGGGAGC
CAGCCTTCTA ACAGCTACCC TTCCATCATA AGTGACTCTT CTGCCCTTGA GGACCTGCGA
AATCCAGAAC AAAGCACATC AGAAAAAGTA TTAACTTCAC AGAAAAGTAG TGAATACCCT
ATAAGCCAGA ATCCAGAAGG CCTTTCTGCT GACAAGTTTG AGGTGTCTGC AGATAGTTCT
ACCAGTAAAA ATAAAGAACC AGGAGTGGAA AGGTCATCCC CTTCTAAATG CCCATCATTA
GATGATAGGT GGTACATGCA CAGTTGCTCT GGGAGTCTTC AGAATAGAAA CTACCCATCT
CAAGAGGAGC TCATTAAGGT TGTTGATGTG GAGGAGCAAC AGCTGGAAGA GTCTGGGCCA
CACGATTTGA CGGAAACATC TTACTTGCCA AGGCAAGATC TAGAGGGAAC CCCTTACCTG
GAATCTGGAA TCAGCCTCTT CTCTGATGAC CCTGAATCTG ATCCTTCTGA AGACAGAGCC
CCAGAGTCAG CTCGTGTTGG CAACATACCA TCTTCAACCT CTGCATTGAA AGTTCCCCAA
TTGAAAGTTG CAGAATCTGC CCAGAGTCCA GCTGCTGCTC ATACTACTGA TACTGCTGGG
TATAATGCAA TGGAAGAAAG TGTGAGCAGG GAGAAGCCAG AATTGACAGC TTCAACAGAA
AGGGTCAACA AAAGAATGTC CATGGTGGTG TCTGGCCTGA CCCCAGAAGA ATTTATGCTC
GTGTACAAGT TTGCCAGAAA ACACCACATC ACTTTAACTA ATCTAATTAC TGAAGAGACT
ACTCATGTTG TTATGAAAAC AGATGCTGAG TTTGTGTGTG AACGGACACT GAAATATTTT
CTAGGAATTG CGGGAGGAAA ATGGGTAGTT AGCTATTTCT GGGTGACCCA GTCTATTAAA
GAAAGAAAAA TGCTGAATGA GCATGATTTT GAAGTCAGAG GAGATGTGGT CAATGGAAGA
AACCACCAAG GTCCAAAGCG AGCAAGAGAA TCCCAGGACA GAAAGATCTT CAGGGGGCTA
GAAATCTGTT GCTATGGGCC CTTCACCAAC ATGCCCACAG GGTGTCCACC CAATTGTGGT
TGTGCAGCCA GATGCCTGGA CAGAGGACAA TGGCTTCCAT GCAATTGGGC AGATGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_009230.2
CDS195..2294
Misc Feature(1)61..61(+)
Misc Feature(2)132..134(+)
Misc Feature(3)261..398(+)
Misc Feature(4)264..386(+)
Misc Feature(5)1830..2054(+)
Misc Feature(6)1869..1907(+)
Misc Feature(7)2034..2048(+)
Exon (1)1..175
Gene:BRCA1
Gene Synonym:
Exon (2)176..274
Gene:BRCA1
Gene Synonym:
Exon (3)275..328
Gene:BRCA1
Gene Synonym:
Exon (4)329..406
Gene:BRCA1
Gene Synonym:
Exon (5)407..495
Gene:BRCA1
Gene Synonym:
Exon (6)496..635
Gene:BRCA1
Gene Synonym:
Exon (7)636..741
Gene:BRCA1
Gene Synonym:
Exon (8)742..787
Gene:BRCA1
Gene Synonym:
Exon (9)788..864
Gene:BRCA1
Gene Synonym:
Exon (10)865..981
Gene:BRCA1
Gene Synonym:
Exon (11)982..1070
Gene:BRCA1
Gene Synonym:
Exon (12)1071..1242
Gene:BRCA1
Gene Synonym:
Exon (13)1243..1366
Gene:BRCA1
Gene Synonym:
Exon (14)1367..1557
Gene:BRCA1
Gene Synonym:
Exon (15)1558..1868
Gene:BRCA1
Gene Synonym:
Exon (16)1869..1956
Gene:BRCA1
Gene Synonym:
Exon (17)1957..2034
Gene:BRCA1
Gene Synonym:
Exon (18)2035..2075
Gene:BRCA1
Gene Synonym:
Exon (19)2076..2159
Gene:BRCA1
Gene Synonym:
Exon (20)2160..2214
Gene:BRCA1
Gene Synonym:
Exon (21)2215..2275
Gene:BRCA1
Gene Synonym:
Exon (22)2276..3783
Gene:BRCA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_007299
Organism Homo sapiens (human)
Definition Homo sapiens breast cancer 1, early onset (BRCA1), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_007299

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGATTTAT CTGCTCTTCG CGTTGAAGAA GTACAAAATG TCATTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCCATCTG TCTGGAGTTG ATCAAGGAAC CTGTCTCCAC AAAGTGTGAC
CACATATTTT GCAAATTTTG CATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAG
TGTCCTTTAT GTAAGAATGA TATAACCAAA AGGAGCCTAC AAGAAAGTAC GAGATTTAGT
CAACTTGTTG AAGAGCTATT GAAAATCATT TGTGCTTTTC AGCTTGACAC AGGTTTGGAG
TATGCAAACA GCTATAATTT TGCAAAAAAG GAAAATAACT CTCCTGAACA TCTAAAAGAT
GAAGTTTCTA TCATCCAAAG TATGGGCTAC AGAAACCGTG CCAAAAGACT TCTACAGAGT
GAACCCGAAA ATCCTTCCTT GCAGGAAACC AGTCTCAGTG TCCAACTCTC TAACCTTGGA
ACTGTGAGAA CTCTGAGGAC AAAGCAGCGG ATACAACCTC AAAAGACGTC TGTCTACATT
GAATTGGGAT CTGATTCTTC TGAAGATACC GTTAATAAGG CAACTTATTG CAGTGTGGGA
GATCAAGAAT TGTTACAAAT CACCCCTCAA GGAACCAGGG ATGAAATCAG TTTGGATTCT
GCAAAAAAGG CTGCTTGTGA ATTTTCTGAG ACGGATGTAA CAAATACTGA ACATCATCAA
CCCAGTAATA ATGATTTGAA CACCACTGAG AAGCGTGCAG CTGAGAGGCA TCCAGAAAAG
TATCAGGGTG AAGCAGCATC TGGGTGTGAG AGTGAAACAA GCGTCTCTGA AGACTGCTCA
GGGCTATCCT CTCAGAGTGA CATTTTAACC ACTCAGCAGA GGGATACCAT GCAACATAAC
CTGATAAAGC TCCAGCAGGA AATGGCTGAA CTAGAAGCTG TGTTAGAACA GCATGGGAGC
CAGCCTTCTA ACAGCTACCC TTCCATCATA AGTGACTCTT CTGCCCTTGA GGACCTGCGA
AATCCAGAAC AAAGCACATC AGAAAAAGTA TTAACTTCAC AGAAAAGTAG TGAATACCCT
ATAAGCCAGA ATCCAGAAGG CCTTTCTGCT GACAAGTTTG AGGTGTCTGC AGATAGTTCT
ACCAGTAAAA ATAAAGAACC AGGAGTGGAA AGGTCATCCC CTTCTAAATG CCCATCATTA
GATGATAGGT GGTACATGCA CAGTTGCTCT GGGAGTCTTC AGAATAGAAA CTACCCATCT
CAAGAGGAGC TCATTAAGGT TGTTGATGTG GAGGAGCAAC AGCTGGAAGA GTCTGGGCCA
CACGATTTGA CGGAAACATC TTACTTGCCA AGGCAAGATC TAGAGGGAAC CCCTTACCTG
GAATCTGGAA TCAGCCTCTT CTCTGATGAC CCTGAATCTG ATCCTTCTGA AGACAGAGCC
CCAGAGTCAG CTCGTGTTGG CAACATACCA TCTTCAACCT CTGCATTGAA AGTTCCCCAA
TTGAAAGTTG CAGAATCTGC CCAGAGTCCA GCTGCTGCTC ATACTACTGA TACTGCTGGG
TATAATGCAA TGGAAGAAAG TGTGAGCAGG GAGAAGCCAG AATTGACAGC TTCAACAGAA
AGGGTCAACA AAAGAATGTC CATGGTGGTG TCTGGCCTGA CCCCAGAAGA ATTTATGCTC
GTGTACAAGT TTGCCAGAAA ACACCACATC ACTTTAACTA ATCTAATTAC TGAAGAGACT
ACTCATGTTG TTATGAAAAC AGATGCTGAG TTTGTGTGTG AACGGACACT GAAATATTTT
CTAGGAATTG CGGGAGGAAA ATGGGTAGTT AGCTATTTCT GGGTGACCCA GTCTATTAAA
GAAAGAAAAA TGCTGAATGA GCATGATTTT GAAGTCAGAG GAGATGTGGT CAATGGAAGA
AACCACCAAG GTCCAAAGCG AGCAAGAGAA TCCCAGGACA GAAAGATCTT CAGGGGGCTA
GAAATCTGTT GCTATGGGCC CTTCACCAAC ATGCCCACAG GGTGTCCACC CAATTGTGGT
TGTGCAGCCA GATGCCTGGA CAGAGGACAA TGGCTTCCAT GCAATTGGGC AGATGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18572
Clone ID Related Accession (Same CDS sequence) NM_007294
Accession Version NM_007294.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5592bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 03-AUG-2015
Organism Homo sapiens (human)
Product breast cancer type 1 susceptibility protein isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL701927.1, U14680.1, BC072418.1, BU617173.1 and BU679389.1. This sequence is a reference standard in the RefSeqGene project. On May 19, 2009 this sequence version replaced gi:63252871. Transcript Variant: This variant (1, also known as BRCA1a) represents the more frequently occurring transcript. It encodes the full-length BRCA1 protein (isoform 1), which is also known as p220. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U14680.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
ATGGATTTAT CTGCTCTTCG CGTTGAAGAA GTACAAAATG TCATTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCCATCTG TCTGGAGTTG ATCAAGGAAC CTGTCTCCAC AAAGTGTGAC
CACATATTTT GCAAATTTTG CATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAG
TGTCCTTTAT GTAAGAATGA TATAACCAAA AGGAGCCTAC AAGAAAGTAC GAGATTTAGT
CAACTTGTTG AAGAGCTATT GAAAATCATT TGTGCTTTTC AGCTTGACAC AGGTTTGGAG
TATGCAAACA GCTATAATTT TGCAAAAAAG GAAAATAACT CTCCTGAACA TCTAAAAGAT
GAAGTTTCTA TCATCCAAAG TATGGGCTAC AGAAACCGTG CCAAAAGACT TCTACAGAGT
GAACCCGAAA ATCCTTCCTT GCAGGAAACC AGTCTCAGTG TCCAACTCTC TAACCTTGGA
ACTGTGAGAA CTCTGAGGAC AAAGCAGCGG ATACAACCTC AAAAGACGTC TGTCTACATT
GAATTGGGAT CTGATTCTTC TGAAGATACC GTTAATAAGG CAACTTATTG CAGTGTGGGA
GATCAAGAAT TGTTACAAAT CACCCCTCAA GGAACCAGGG ATGAAATCAG TTTGGATTCT
GCAAAAAAGG CTGCTTGTGA ATTTTCTGAG ACGGATGTAA CAAATACTGA ACATCATCAA
CCCAGTAATA ATGATTTGAA CACCACTGAG AAGCGTGCAG CTGAGAGGCA TCCAGAAAAG
TATCAGGGTA GTTCTGTTTC AAACTTGCAT GTGGAGCCAT GTGGCACAAA TACTCATGCC
AGCTCATTAC AGCATGAGAA CAGCAGTTTA TTACTCACTA AAGACAGAAT GAATGTAGAA
AAGGCTGAAT TCTGTAATAA AAGCAAACAG CCTGGCTTAG CAAGGAGCCA ACATAACAGA
TGGGCTGGAA GTAAGGAAAC ATGTAATGAT AGGCGGACTC CCAGCACAGA AAAAAAGGTA
GATCTGAATG CTGATCCCCT GTGTGAGAGA AAAGAATGGA ATAAGCAGAA ACTGCCATGC
TCAGAGAATC CTAGAGATAC TGAAGATGTT CCTTGGATAA CACTAAATAG CAGCATTCAG
AAAGTTAATG AGTGGTTTTC CAGAAGTGAT GAACTGTTAG GTTCTGATGA CTCACATGAT
GGGGAGTCTG AATCAAATGC CAAAGTAGCT GATGTATTGG ACGTTCTAAA TGAGGTAGAT
GAATATTCTG GTTCTTCAGA GAAAATAGAC TTACTGGCCA GTGATCCTCA TGAGGCTTTA
ATATGTAAAA GTGAAAGAGT TCACTCCAAA TCAGTAGAGA GTAATATTGA AGACAAAATA
TTTGGGAAAA CCTATCGGAA GAAGGCAAGC CTCCCCAACT TAAGCCATGT AACTGAAAAT
CTAATTATAG GAGCATTTGT TACTGAGCCA CAGATAATAC AAGAGCGTCC CCTCACAAAT
AAATTAAAGC GTAAAAGGAG ACCTACATCA GGCCTTCATC CTGAGGATTT TATCAAGAAA
GCAGATTTGG CAGTTCAAAA GACTCCTGAA ATGATAAATC AGGGAACTAA CCAAACGGAG
CAGAATGGTC AAGTGATGAA TATTACTAAT AGTGGTCATG AGAATAAAAC AAAAGGTGAT
TCTATTCAGA ATGAGAAAAA TCCTAACCCA ATAGAATCAC TCGAAAAAGA ATCTGCTTTC
AAAACGAAAG CTGAACCTAT AAGCAGCAGT ATAAGCAATA TGGAACTCGA ATTAAATATC
CACAATTCAA AAGCACCTAA AAAGAATAGG CTGAGGAGGA AGTCTTCTAC CAGGCATATT
CATGCGCTTG AACTAGTAGT CAGTAGAAAT CTAAGCCCAC CTAATTGTAC TGAATTGCAA
ATTGATAGTT GTTCTAGCAG TGAAGAGATA AAGAAAAAAA AGTACAACCA AATGCCAGTC
AGGCACAGCA GAAACCTACA ACTCATGGAA GGTAAAGAAC CTGCAACTGG AGCCAAGAAG
AGTAACAAGC CAAATGAACA GACAAGTAAA AGACATGACA GCGATACTTT CCCAGAGCTG
AAGTTAACAA ATGCACCTGG TTCTTTTACT AAGTGTTCAA ATACCAGTGA ACTTAAAGAA
TTTGTCAATC CTAGCCTTCC AAGAGAAGAA AAAGAAGAGA AACTAGAAAC AGTTAAAGTG
TCTAATAATG CTGAAGACCC CAAAGATCTC ATGTTAAGTG GAGAAAGGGT TTTGCAAACT
GAAAGATCTG TAGAGAGTAG CAGTATTTCA TTGGTACCTG GTACTGATTA TGGCACTCAG
GAAAGTATCT CGTTACTGGA AGTTAGCACT CTAGGGAAGG CAAAAACAGA ACCAAATAAA
TGTGTGAGTC AGTGTGCAGC ATTTGAAAAC CCCAAGGGAC TAATTCATGG TTGTTCCAAA
GATAATAGAA ATGACACAGA AGGCTTTAAG TATCCATTGG GACATGAAGT TAACCACAGT
CGGGAAACAA GCATAGAAAT GGAAGAAAGT GAACTTGATG CTCAGTATTT GCAGAATACA
TTCAAGGTTT CAAAGCGCCA GTCATTTGCT CCGTTTTCAA ATCCAGGAAA TGCAGAAGAG
GAATGTGCAA CATTCTCTGC CCACTCTGGG TCCTTAAAGA AACAAAGTCC AAAAGTCACT
TTTGAATGTG AACAAAAGGA AGAAAATCAA GGAAAGAATG AGTCTAATAT CAAGCCTGTA
CAGACAGTTA ATATCACTGC AGGCTTTCCT GTGGTTGGTC AGAAAGATAA GCCAGTTGAT
AATGCCAAAT GTAGTATCAA AGGAGGCTCT AGGTTTTGTC TATCATCTCA GTTCAGAGGC
AACGAAACTG GACTCATTAC TCCAAATAAA CATGGACTTT TACAAAACCC ATATCGTATA
CCACCACTTT TTCCCATCAA GTCATTTGTT AAAACTAAAT GTAAGAAAAA TCTGCTAGAG
GAAAACTTTG AGGAACATTC AATGTCACCT GAAAGAGAAA TGGGAAATGA GAACATTCCA
AGTACAGTGA GCACAATTAG CCGTAATAAC ATTAGAGAAA ATGTTTTTAA AGAAGCCAGC
TCAAGCAATA TTAATGAAGT AGGTTCCAGT ACTAATGAAG TGGGCTCCAG TATTAATGAA
ATAGGTTCCA GTGATGAAAA CATTCAAGCA GAACTAGGTA GAAACAGAGG GCCAAAATTG
AATGCTATGC TTAGATTAGG GGTTTTGCAA CCTGAGGTCT ATAAACAAAG TCTTCCTGGA
AGTAATTGTA AGCATCCTGA AATAAAAAAG CAAGAATATG AAGAAGTAGT TCAGACTGTT
AATACAGATT TCTCTCCATA TCTGATTTCA GATAACTTAG AACAGCCTAT GGGAAGTAGT
CATGCATCTC AGGTTTGTTC TGAGACACCT GATGACCTGT TAGATGATGG TGAAATAAAG
GAAGATACTA GTTTTGCTGA AAATGACATT AAGGAAAGTT CTGCTGTTTT TAGCAAAAGC
GTCCAGAAAG GAGAGCTTAG CAGGAGTCCT AGCCCTTTCA CCCATACACA TTTGGCTCAG
GGTTACCGAA GAGGGGCCAA GAAATTAGAG TCCTCAGAAG AGAACTTATC TAGTGAGGAT
GAAGAGCTTC CCTGCTTCCA ACACTTGTTA TTTGGTAAAG TAAACAATAT ACCTTCTCAG
TCTACTAGGC ATAGCACCGT TGCTACCGAG TGTCTGTCTA AGAACACAGA GGAGAATTTA
TTATCATTGA AGAATAGCTT AAATGACTGC AGTAACCAGG TAATATTGGC AAAGGCATCT
CAGGAACATC ACCTTAGTGA GGAAACAAAA TGTTCTGCTA GCTTGTTTTC TTCACAGTGC
AGTGAATTGG AAGACTTGAC TGCAAATACA AACACCCAGG ATCCTTTCTT GATTGGTTCT
TCCAAACAAA TGAGGCATCA GTCTGAAAGC CAGGGAGTTG GTCTGAGTGA CAAGGAATTG
GTTTCAGATG ATGAAGAAAG AGGAACGGGC TTGGAAGAAA ATAATCAAGA AGAGCAAAGC
ATGGATTCAA ACTTAGGTGA AGCAGCATCT GGGTGTGAGA GTGAAACAAG CGTCTCTGAA
GACTGCTCAG GGCTATCCTC TCAGAGTGAC ATTTTAACCA CTCAGCAGAG GGATACCATG
CAACATAACC TGATAAAGCT CCAGCAGGAA ATGGCTGAAC TAGAAGCTGT GTTAGAACAG
CATGGGAGCC AGCCTTCTAA CAGCTACCCT TCCATCATAA GTGACTCTTC TGCCCTTGAG
GACCTGCGAA ATCCAGAACA AAGCACATCA GAAAAAGCAG TATTAACTTC ACAGAAAAGT
AGTGAATACC CTATAAGCCA GAATCCAGAA GGCCTTTCTG CTGACAAGTT TGAGGTGTCT
GCAGATAGTT CTACCAGTAA AAATAAAGAA CCAGGAGTGG AAAGGTCATC CCCTTCTAAA
TGCCCATCAT TAGATGATAG GTGGTACATG CACAGTTGCT CTGGGAGTCT TCAGAATAGA
AACTACCCAT CTCAAGAGGA GCTCATTAAG GTTGTTGATG TGGAGGAGCA ACAGCTGGAA
GAGTCTGGGC CACACGATTT GACGGAAACA TCTTACTTGC CAAGGCAAGA TCTAGAGGGA
ACCCCTTACC TGGAATCTGG AATCAGCCTC TTCTCTGATG ACCCTGAATC TGATCCTTCT
GAAGACAGAG CCCCAGAGTC AGCTCGTGTT GGCAACATAC CATCTTCAAC CTCTGCATTG
AAAGTTCCCC AATTGAAAGT TGCAGAATCT GCCCAGAGTC CAGCTGCTGC TCATACTACT
GATACTGCTG GGTATAATGC AATGGAAGAA AGTGTGAGCA GGGAGAAGCC AGAATTGACA
GCTTCAACAG AAAGGGTCAA CAAAAGAATG TCCATGGTGG TGTCTGGCCT GACCCCAGAA
GAATTTATGC TCGTGTACAA GTTTGCCAGA AAACACCACA TCACTTTAAC TAATCTAATT
ACTGAAGAGA CTACTCATGT TGTTATGAAA ACAGATGCTG AGTTTGTGTG TGAACGGACA
CTGAAATATT TTCTAGGAAT TGCGGGAGGA AAATGGGTAG TTAGCTATTT CTGGGTGACC
CAGTCTATTA AAGAAAGAAA AATGCTGAAT GAGCATGATT TTGAAGTCAG AGGAGATGTG
GTCAATGGAA GAAACCACCA AGGTCCAAAG CGAGCAAGAG AATCCCAGGA CAGAAAGATC
TTCAGGGGGC TAGAAATCTG TTGCTATGGG CCCTTCACCA ACATGCCCAC AGATCAACTG
GAATGGATGG TACAGCTGTG TGGTGCTTCT GTGGTGAAGG AGCTTTCATC ATTCACCCTT
GGCACAGGTG TCCACCCAAT TGTGGTTGTG CAGCCAGATG CCTGGACAGA GGACAATGGC
TTCCATGCAA TTGGGCAGAT GTGTGAGGCA CCTGTGGTGA CCCGAGAGTG GGTGTTGGAC
AGTGTAGCAC TCTACCAGTG CCAGGAGCTG GACACCTACC TGATACCCCA GATCCCCCAC
AGCCACTACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_009225.1
CDS233..5824
Misc Feature(1)93..93(+)
Misc Feature(2)209..211(+)
Misc Feature(3)233..235(+)
Misc Feature(4)299..436(+)
Misc Feature(5)302..424(+)
Misc Feature(6)572..574(+)
Misc Feature(7)1154..1156(+)
Misc Feature(8)1154..1156(+)
Misc Feature(9)1262..1753(+)
Misc Feature(10)1415..1417(+)
Misc Feature(11)1424..1426(+)
Misc Feature(12)1499..1501(+)
Misc Feature(13)1757..1759(+)
Misc Feature(14)2174..3166(+)
Misc Feature(15)2312..2314(+)
Misc Feature(16)2489..2491(+)
Misc Feature(17)2750..2752(+)
Misc Feature(18)3194..3196(+)
Misc Feature(19)3194..3196(+)
Misc Feature(20)3659..3661(+)
Misc Feature(21)3659..3661(+)
Misc Feature(22)3695..3697(+)
Misc Feature(23)3797..3799(+)
Misc Feature(24)3863..3865(+)
Misc Feature(25)3863..3865(+)
Misc Feature(26)3866..3868(+)
Misc Feature(27)3881..3883(+)
Misc Feature(28)3881..3883(+)
Misc Feature(29)3884..3886(+)
Misc Feature(30)3884..3886(+)
Misc Feature(31)3947..3949(+)
Misc Feature(32)3965..3967(+)
Misc Feature(33)4070..4072(+)
Misc Feature(34)4070..4072(+)
Misc Feature(35)4214..4216(+)
Misc Feature(36)4220..4222(+)
Misc Feature(37)4238..4240(+)
Misc Feature(38)4238..4240(+)
Misc Feature(39)4256..4258(+)
Misc Feature(40)4256..4258(+)
Misc Feature(41)4391..4393(+)
Misc Feature(42)4391..4393(+)
Misc Feature(43)4391..4393(+)
Misc Feature(44)4412..4414(+)
Misc Feature(45)4412..4414(+)
Misc Feature(46)4421..4504(+)
Misc Feature(47)4499..4501(+)
Misc Feature(48)4499..4501(+)
Misc Feature(49)4499..4501(+)
Misc Feature(50)4601..4603(+)
Misc Feature(51)4601..4603(+)
Misc Feature(52)4601..4603(+)
Misc Feature(53)4628..4630(+)
Misc Feature(54)4721..4723(+)
Misc Feature(55)4727..4729(+)
Misc Feature(56)4802..4804(+)
Misc Feature(57)4802..4804(+)
Misc Feature(58)4802..4804(+)
Misc Feature(59)4856..4858(+)
Misc Feature(60)4946..4948(+)
Misc Feature(61)5180..5404(+)
Misc Feature(62)5219..5257(+)
Misc Feature(63)5330..5332(+)
Misc Feature(64)5384..5398(+)
Misc Feature(65)5390..5392(+)
Misc Feature(66)5519..5758(+)
Misc Feature(67)5564..5602(+)
Misc Feature(68)5741..5755(+)
Exon (1)1..213
Gene:BRCA1
Gene Synonym:
Exon (2)214..312
Gene:BRCA1
Gene Synonym:
Exon (3)313..366
Gene:BRCA1
Gene Synonym:
Exon (4)367..444
Gene:BRCA1
Gene Synonym:
Exon (5)445..533
Gene:BRCA1
Gene Synonym:
Exon (6)534..673
Gene:BRCA1
Gene Synonym:
Exon (7)674..779
Gene:BRCA1
Gene Synonym:
Exon (8)780..825
Gene:BRCA1
Gene Synonym:
Exon (9)826..902
Gene:BRCA1
Gene Synonym:
Exon (10)903..4328
Gene:BRCA1
Gene Synonym:
Exon (11)4329..4417
Gene:BRCA1
Gene Synonym:
Exon (12)4418..4589
Gene:BRCA1
Gene Synonym:
Exon (13)4590..4716
Gene:BRCA1
Gene Synonym:
Exon (14)4717..4907
Gene:BRCA1
Gene Synonym:
Exon (15)4908..5218
Gene:BRCA1
Gene Synonym:
Exon (16)5219..5306
Gene:BRCA1
Gene Synonym:
Exon (17)5307..5384
Gene:BRCA1
Gene Synonym:
Exon (18)5385..5425
Gene:BRCA1
Gene Synonym:
Exon (19)5426..5509
Gene:BRCA1
Gene Synonym:
Exon (20)5510..5564
Gene:BRCA1
Gene Synonym:
Exon (21)5565..5638
Gene:BRCA1
Gene Synonym:
Exon (22)5639..5699
Gene:BRCA1
Gene Synonym:
Exon (23)5700..7207
Gene:BRCA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_007294
Organism Homo sapiens (human)
Definition Homo sapiens breast cancer 1, early onset (BRCA1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_007294

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
ATGGATTTAT CTGCTCTTCG CGTTGAAGAA GTACAAAATG TCATTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCCATCTG TCTGGAGTTG ATCAAGGAAC CTGTCTCCAC AAAGTGTGAC
CACATATTTT GCAAATTTTG CATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAG
TGTCCTTTAT GTAAGAATGA TATAACCAAA AGGAGCCTAC AAGAAAGTAC GAGATTTAGT
CAACTTGTTG AAGAGCTATT GAAAATCATT TGTGCTTTTC AGCTTGACAC AGGTTTGGAG
TATGCAAACA GCTATAATTT TGCAAAAAAG GAAAATAACT CTCCTGAACA TCTAAAAGAT
GAAGTTTCTA TCATCCAAAG TATGGGCTAC AGAAACCGTG CCAAAAGACT TCTACAGAGT
GAACCCGAAA ATCCTTCCTT GCAGGAAACC AGTCTCAGTG TCCAACTCTC TAACCTTGGA
ACTGTGAGAA CTCTGAGGAC AAAGCAGCGG ATACAACCTC AAAAGACGTC TGTCTACATT
GAATTGGGAT CTGATTCTTC TGAAGATACC GTTAATAAGG CAACTTATTG CAGTGTGGGA
GATCAAGAAT TGTTACAAAT CACCCCTCAA GGAACCAGGG ATGAAATCAG TTTGGATTCT
GCAAAAAAGG CTGCTTGTGA ATTTTCTGAG ACGGATGTAA CAAATACTGA ACATCATCAA
CCCAGTAATA ATGATTTGAA CACCACTGAG AAGCGTGCAG CTGAGAGGCA TCCAGAAAAG
TATCAGGGTA GTTCTGTTTC AAACTTGCAT GTGGAGCCAT GTGGCACAAA TACTCATGCC
AGCTCATTAC AGCATGAGAA CAGCAGTTTA TTACTCACTA AAGACAGAAT GAATGTAGAA
AAGGCTGAAT TCTGTAATAA AAGCAAACAG CCTGGCTTAG CAAGGAGCCA ACATAACAGA
TGGGCTGGAA GTAAGGAAAC ATGTAATGAT AGGCGGACTC CCAGCACAGA AAAAAAGGTA
GATCTGAATG CTGATCCCCT GTGTGAGAGA AAAGAATGGA ATAAGCAGAA ACTGCCATGC
TCAGAGAATC CTAGAGATAC TGAAGATGTT CCTTGGATAA CACTAAATAG CAGCATTCAG
AAAGTTAATG AGTGGTTTTC CAGAAGTGAT GAACTGTTAG GTTCTGATGA CTCACATGAT
GGGGAGTCTG AATCAAATGC CAAAGTAGCT GATGTATTGG ACGTTCTAAA TGAGGTAGAT
GAATATTCTG GTTCTTCAGA GAAAATAGAC TTACTGGCCA GTGATCCTCA TGAGGCTTTA
ATATGTAAAA GTGAAAGAGT TCACTCCAAA TCAGTAGAGA GTAATATTGA AGACAAAATA
TTTGGGAAAA CCTATCGGAA GAAGGCAAGC CTCCCCAACT TAAGCCATGT AACTGAAAAT
CTAATTATAG GAGCATTTGT TACTGAGCCA CAGATAATAC AAGAGCGTCC CCTCACAAAT
AAATTAAAGC GTAAAAGGAG ACCTACATCA GGCCTTCATC CTGAGGATTT TATCAAGAAA
GCAGATTTGG CAGTTCAAAA GACTCCTGAA ATGATAAATC AGGGAACTAA CCAAACGGAG
CAGAATGGTC AAGTGATGAA TATTACTAAT AGTGGTCATG AGAATAAAAC AAAAGGTGAT
TCTATTCAGA ATGAGAAAAA TCCTAACCCA ATAGAATCAC TCGAAAAAGA ATCTGCTTTC
AAAACGAAAG CTGAACCTAT AAGCAGCAGT ATAAGCAATA TGGAACTCGA ATTAAATATC
CACAATTCAA AAGCACCTAA AAAGAATAGG CTGAGGAGGA AGTCTTCTAC CAGGCATATT
CATGCGCTTG AACTAGTAGT CAGTAGAAAT CTAAGCCCAC CTAATTGTAC TGAATTGCAA
ATTGATAGTT GTTCTAGCAG TGAAGAGATA AAGAAAAAAA AGTACAACCA AATGCCAGTC
AGGCACAGCA GAAACCTACA ACTCATGGAA GGTAAAGAAC CTGCAACTGG AGCCAAGAAG
AGTAACAAGC CAAATGAACA GACAAGTAAA AGACATGACA GCGATACTTT CCCAGAGCTG
AAGTTAACAA ATGCACCTGG TTCTTTTACT AAGTGTTCAA ATACCAGTGA ACTTAAAGAA
TTTGTCAATC CTAGCCTTCC AAGAGAAGAA AAAGAAGAGA AACTAGAAAC AGTTAAAGTG
TCTAATAATG CTGAAGACCC CAAAGATCTC ATGTTAAGTG GAGAAAGGGT TTTGCAAACT
GAAAGATCTG TAGAGAGTAG CAGTATTTCA TTGGTACCTG GTACTGATTA TGGCACTCAG
GAAAGTATCT CGTTACTGGA AGTTAGCACT CTAGGGAAGG CAAAAACAGA ACCAAATAAA
TGTGTGAGTC AGTGTGCAGC ATTTGAAAAC CCCAAGGGAC TAATTCATGG TTGTTCCAAA
GATAATAGAA ATGACACAGA AGGCTTTAAG TATCCATTGG GACATGAAGT TAACCACAGT
CGGGAAACAA GCATAGAAAT GGAAGAAAGT GAACTTGATG CTCAGTATTT GCAGAATACA
TTCAAGGTTT CAAAGCGCCA GTCATTTGCT CCGTTTTCAA ATCCAGGAAA TGCAGAAGAG
GAATGTGCAA CATTCTCTGC CCACTCTGGG TCCTTAAAGA AACAAAGTCC AAAAGTCACT
TTTGAATGTG AACAAAAGGA AGAAAATCAA GGAAAGAATG AGTCTAATAT CAAGCCTGTA
CAGACAGTTA ATATCACTGC AGGCTTTCCT GTGGTTGGTC AGAAAGATAA GCCAGTTGAT
AATGCCAAAT GTAGTATCAA AGGAGGCTCT AGGTTTTGTC TATCATCTCA GTTCAGAGGC
AACGAAACTG GACTCATTAC TCCAAATAAA CATGGACTTT TACAAAACCC ATATCGTATA
CCACCACTTT TTCCCATCAA GTCATTTGTT AAAACTAAAT GTAAGAAAAA TCTGCTAGAG
GAAAACTTTG AGGAACATTC AATGTCACCT GAAAGAGAAA TGGGAAATGA GAACATTCCA
AGTACAGTGA GCACAATTAG CCGTAATAAC ATTAGAGAAA ATGTTTTTAA AGAAGCCAGC
TCAAGCAATA TTAATGAAGT AGGTTCCAGT ACTAATGAAG TGGGCTCCAG TATTAATGAA
ATAGGTTCCA GTGATGAAAA CATTCAAGCA GAACTAGGTA GAAACAGAGG GCCAAAATTG
AATGCTATGC TTAGATTAGG GGTTTTGCAA CCTGAGGTCT ATAAACAAAG TCTTCCTGGA
AGTAATTGTA AGCATCCTGA AATAAAAAAG CAAGAATATG AAGAAGTAGT TCAGACTGTT
AATACAGATT TCTCTCCATA TCTGATTTCA GATAACTTAG AACAGCCTAT GGGAAGTAGT
CATGCATCTC AGGTTTGTTC TGAGACACCT GATGACCTGT TAGATGATGG TGAAATAAAG
GAAGATACTA GTTTTGCTGA AAATGACATT AAGGAAAGTT CTGCTGTTTT TAGCAAAAGC
GTCCAGAAAG GAGAGCTTAG CAGGAGTCCT AGCCCTTTCA CCCATACACA TTTGGCTCAG
GGTTACCGAA GAGGGGCCAA GAAATTAGAG TCCTCAGAAG AGAACTTATC TAGTGAGGAT
GAAGAGCTTC CCTGCTTCCA ACACTTGTTA TTTGGTAAAG TAAACAATAT ACCTTCTCAG
TCTACTAGGC ATAGCACCGT TGCTACCGAG TGTCTGTCTA AGAACACAGA GGAGAATTTA
TTATCATTGA AGAATAGCTT AAATGACTGC AGTAACCAGG TAATATTGGC AAAGGCATCT
CAGGAACATC ACCTTAGTGA GGAAACAAAA TGTTCTGCTA GCTTGTTTTC TTCACAGTGC
AGTGAATTGG AAGACTTGAC TGCAAATACA AACACCCAGG ATCCTTTCTT GATTGGTTCT
TCCAAACAAA TGAGGCATCA GTCTGAAAGC CAGGGAGTTG GTCTGAGTGA CAAGGAATTG
GTTTCAGATG ATGAAGAAAG AGGAACGGGC TTGGAAGAAA ATAATCAAGA AGAGCAAAGC
ATGGATTCAA ACTTAGGTGA AGCAGCATCT GGGTGTGAGA GTGAAACAAG CGTCTCTGAA
GACTGCTCAG GGCTATCCTC TCAGAGTGAC ATTTTAACCA CTCAGCAGAG GGATACCATG
CAACATAACC TGATAAAGCT CCAGCAGGAA ATGGCTGAAC TAGAAGCTGT GTTAGAACAG
CATGGGAGCC AGCCTTCTAA CAGCTACCCT TCCATCATAA GTGACTCTTC TGCCCTTGAG
GACCTGCGAA ATCCAGAACA AAGCACATCA GAAAAAGCAG TATTAACTTC ACAGAAAAGT
AGTGAATACC CTATAAGCCA GAATCCAGAA GGCCTTTCTG CTGACAAGTT TGAGGTGTCT
GCAGATAGTT CTACCAGTAA AAATAAAGAA CCAGGAGTGG AAAGGTCATC CCCTTCTAAA
TGCCCATCAT TAGATGATAG GTGGTACATG CACAGTTGCT CTGGGAGTCT TCAGAATAGA
AACTACCCAT CTCAAGAGGA GCTCATTAAG GTTGTTGATG TGGAGGAGCA ACAGCTGGAA
GAGTCTGGGC CACACGATTT GACGGAAACA TCTTACTTGC CAAGGCAAGA TCTAGAGGGA
ACCCCTTACC TGGAATCTGG AATCAGCCTC TTCTCTGATG ACCCTGAATC TGATCCTTCT
GAAGACAGAG CCCCAGAGTC AGCTCGTGTT GGCAACATAC CATCTTCAAC CTCTGCATTG
AAAGTTCCCC AATTGAAAGT TGCAGAATCT GCCCAGAGTC CAGCTGCTGC TCATACTACT
GATACTGCTG GGTATAATGC AATGGAAGAA AGTGTGAGCA GGGAGAAGCC AGAATTGACA
GCTTCAACAG AAAGGGTCAA CAAAAGAATG TCCATGGTGG TGTCTGGCCT GACCCCAGAA
GAATTTATGC TCGTGTACAA GTTTGCCAGA AAACACCACA TCACTTTAAC TAATCTAATT
ACTGAAGAGA CTACTCATGT TGTTATGAAA ACAGATGCTG AGTTTGTGTG TGAACGGACA
CTGAAATATT TTCTAGGAAT TGCGGGAGGA AAATGGGTAG TTAGCTATTT CTGGGTGACC
CAGTCTATTA AAGAAAGAAA AATGCTGAAT GAGCATGATT TTGAAGTCAG AGGAGATGTG
GTCAATGGAA GAAACCACCA AGGTCCAAAG CGAGCAAGAG AATCCCAGGA CAGAAAGATC
TTCAGGGGGC TAGAAATCTG TTGCTATGGG CCCTTCACCA ACATGCCCAC AGATCAACTG
GAATGGATGG TACAGCTGTG TGGTGCTTCT GTGGTGAAGG AGCTTTCATC ATTCACCCTT
GGCACAGGTG TCCACCCAAT TGTGGTTGTG CAGCCAGATG CCTGGACAGA GGACAATGGC
TTCCATGCAA TTGGGCAGAT GTGTGAGGCA CCTGTGGTGA CCCGAGAGTG GGTGTTGGAC
AGTGTAGCAC TCTACCAGTG CCAGGAGCTG GACACCTACC TGATACCCCA GATCCCCCAC
AGCCACTACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23229
Clone ID Related Accession (Same CDS sequence) NM_007300
Accession Version NM_007300.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5655bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 03-AUG-2015
Organism Homo sapiens (human)
Product breast cancer type 1 susceptibility protein isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL701927.1, BC115037.1, U14680.1, AC135721.4, BC072418.1, BU617173.1 and BU679389.1. On May 16, 2009 this sequence version replaced gi:63252877. Transcript Variant: This variant (2) includes an alternate in-frame exon and an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC115037.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
ATGGATTTAT CTGCTCTTCG CGTTGAAGAA GTACAAAATG TCATTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCCATCTG TCTGGAGTTG ATCAAGGAAC CTGTCTCCAC AAAGTGTGAC
CACATATTTT GCAAATTTTG CATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAG
TGTCCTTTAT GTAAGAATGA TATAACCAAA AGGAGCCTAC AAGAAAGTAC GAGATTTAGT
CAACTTGTTG AAGAGCTATT GAAAATCATT TGTGCTTTTC AGCTTGACAC AGGTTTGGAG
TATGCAAACA GCTATAATTT TGCAAAAAAG GAAAATAACT CTCCTGAACA TCTAAAAGAT
GAAGTTTCTA TCATCCAAAG TATGGGCTAC AGAAACCGTG CCAAAAGACT TCTACAGAGT
GAACCCGAAA ATCCTTCCTT GCAGGAAACC AGTCTCAGTG TCCAACTCTC TAACCTTGGA
ACTGTGAGAA CTCTGAGGAC AAAGCAGCGG ATACAACCTC AAAAGACGTC TGTCTACATT
GAATTGGGAT CTGATTCTTC TGAAGATACC GTTAATAAGG CAACTTATTG CAGTGTGGGA
GATCAAGAAT TGTTACAAAT CACCCCTCAA GGAACCAGGG ATGAAATCAG TTTGGATTCT
GCAAAAAAGG CTGCTTGTGA ATTTTCTGAG ACGGATGTAA CAAATACTGA ACATCATCAA
CCCAGTAATA ATGATTTGAA CACCACTGAG AAGCGTGCAG CTGAGAGGCA TCCAGAAAAG
TATCAGGGTA GTTCTGTTTC AAACTTGCAT GTGGAGCCAT GTGGCACAAA TACTCATGCC
AGCTCATTAC AGCATGAGAA CAGCAGTTTA TTACTCACTA AAGACAGAAT GAATGTAGAA
AAGGCTGAAT TCTGTAATAA AAGCAAACAG CCTGGCTTAG CAAGGAGCCA ACATAACAGA
TGGGCTGGAA GTAAGGAAAC ATGTAATGAT AGGCGGACTC CCAGCACAGA AAAAAAGGTA
GATCTGAATG CTGATCCCCT GTGTGAGAGA AAAGAATGGA ATAAGCAGAA ACTGCCATGC
TCAGAGAATC CTAGAGATAC TGAAGATGTT CCTTGGATAA CACTAAATAG CAGCATTCAG
AAAGTTAATG AGTGGTTTTC CAGAAGTGAT GAACTGTTAG GTTCTGATGA CTCACATGAT
GGGGAGTCTG AATCAAATGC CAAAGTAGCT GATGTATTGG ACGTTCTAAA TGAGGTAGAT
GAATATTCTG GTTCTTCAGA GAAAATAGAC TTACTGGCCA GTGATCCTCA TGAGGCTTTA
ATATGTAAAA GTGAAAGAGT TCACTCCAAA TCAGTAGAGA GTAATATTGA AGACAAAATA
TTTGGGAAAA CCTATCGGAA GAAGGCAAGC CTCCCCAACT TAAGCCATGT AACTGAAAAT
CTAATTATAG GAGCATTTGT TACTGAGCCA CAGATAATAC AAGAGCGTCC CCTCACAAAT
AAATTAAAGC GTAAAAGGAG ACCTACATCA GGCCTTCATC CTGAGGATTT TATCAAGAAA
GCAGATTTGG CAGTTCAAAA GACTCCTGAA ATGATAAATC AGGGAACTAA CCAAACGGAG
CAGAATGGTC AAGTGATGAA TATTACTAAT AGTGGTCATG AGAATAAAAC AAAAGGTGAT
TCTATTCAGA ATGAGAAAAA TCCTAACCCA ATAGAATCAC TCGAAAAAGA ATCTGCTTTC
AAAACGAAAG CTGAACCTAT AAGCAGCAGT ATAAGCAATA TGGAACTCGA ATTAAATATC
CACAATTCAA AAGCACCTAA AAAGAATAGG CTGAGGAGGA AGTCTTCTAC CAGGCATATT
CATGCGCTTG AACTAGTAGT CAGTAGAAAT CTAAGCCCAC CTAATTGTAC TGAATTGCAA
ATTGATAGTT GTTCTAGCAG TGAAGAGATA AAGAAAAAAA AGTACAACCA AATGCCAGTC
AGGCACAGCA GAAACCTACA ACTCATGGAA GGTAAAGAAC CTGCAACTGG AGCCAAGAAG
AGTAACAAGC CAAATGAACA GACAAGTAAA AGACATGACA GCGATACTTT CCCAGAGCTG
AAGTTAACAA ATGCACCTGG TTCTTTTACT AAGTGTTCAA ATACCAGTGA ACTTAAAGAA
TTTGTCAATC CTAGCCTTCC AAGAGAAGAA AAAGAAGAGA AACTAGAAAC AGTTAAAGTG
TCTAATAATG CTGAAGACCC CAAAGATCTC ATGTTAAGTG GAGAAAGGGT TTTGCAAACT
GAAAGATCTG TAGAGAGTAG CAGTATTTCA TTGGTACCTG GTACTGATTA TGGCACTCAG
GAAAGTATCT CGTTACTGGA AGTTAGCACT CTAGGGAAGG CAAAAACAGA ACCAAATAAA
TGTGTGAGTC AGTGTGCAGC ATTTGAAAAC CCCAAGGGAC TAATTCATGG TTGTTCCAAA
GATAATAGAA ATGACACAGA AGGCTTTAAG TATCCATTGG GACATGAAGT TAACCACAGT
CGGGAAACAA GCATAGAAAT GGAAGAAAGT GAACTTGATG CTCAGTATTT GCAGAATACA
TTCAAGGTTT CAAAGCGCCA GTCATTTGCT CCGTTTTCAA ATCCAGGAAA TGCAGAAGAG
GAATGTGCAA CATTCTCTGC CCACTCTGGG TCCTTAAAGA AACAAAGTCC AAAAGTCACT
TTTGAATGTG AACAAAAGGA AGAAAATCAA GGAAAGAATG AGTCTAATAT CAAGCCTGTA
CAGACAGTTA ATATCACTGC AGGCTTTCCT GTGGTTGGTC AGAAAGATAA GCCAGTTGAT
AATGCCAAAT GTAGTATCAA AGGAGGCTCT AGGTTTTGTC TATCATCTCA GTTCAGAGGC
AACGAAACTG GACTCATTAC TCCAAATAAA CATGGACTTT TACAAAACCC ATATCGTATA
CCACCACTTT TTCCCATCAA GTCATTTGTT AAAACTAAAT GTAAGAAAAA TCTGCTAGAG
GAAAACTTTG AGGAACATTC AATGTCACCT GAAAGAGAAA TGGGAAATGA GAACATTCCA
AGTACAGTGA GCACAATTAG CCGTAATAAC ATTAGAGAAA ATGTTTTTAA AGAAGCCAGC
TCAAGCAATA TTAATGAAGT AGGTTCCAGT ACTAATGAAG TGGGCTCCAG TATTAATGAA
ATAGGTTCCA GTGATGAAAA CATTCAAGCA GAACTAGGTA GAAACAGAGG GCCAAAATTG
AATGCTATGC TTAGATTAGG GGTTTTGCAA CCTGAGGTCT ATAAACAAAG TCTTCCTGGA
AGTAATTGTA AGCATCCTGA AATAAAAAAG CAAGAATATG AAGAAGTAGT TCAGACTGTT
AATACAGATT TCTCTCCATA TCTGATTTCA GATAACTTAG AACAGCCTAT GGGAAGTAGT
CATGCATCTC AGGTTTGTTC TGAGACACCT GATGACCTGT TAGATGATGG TGAAATAAAG
GAAGATACTA GTTTTGCTGA AAATGACATT AAGGAAAGTT CTGCTGTTTT TAGCAAAAGC
GTCCAGAAAG GAGAGCTTAG CAGGAGTCCT AGCCCTTTCA CCCATACACA TTTGGCTCAG
GGTTACCGAA GAGGGGCCAA GAAATTAGAG TCCTCAGAAG AGAACTTATC TAGTGAGGAT
GAAGAGCTTC CCTGCTTCCA ACACTTGTTA TTTGGTAAAG TAAACAATAT ACCTTCTCAG
TCTACTAGGC ATAGCACCGT TGCTACCGAG TGTCTGTCTA AGAACACAGA GGAGAATTTA
TTATCATTGA AGAATAGCTT AAATGACTGC AGTAACCAGG TAATATTGGC AAAGGCATCT
CAGGAACATC ACCTTAGTGA GGAAACAAAA TGTTCTGCTA GCTTGTTTTC TTCACAGTGC
AGTGAATTGG AAGACTTGAC TGCAAATACA AACACCCAGG ATCCTTTCTT GATTGGTTCT
TCCAAACAAA TGAGGCATCA GTCTGAAAGC CAGGGAGTTG GTCTGAGTGA CAAGGAATTG
GTTTCAGATG ATGAAGAAAG AGGAACGGGC TTGGAAGAAA ATAATCAAGA AGAGCAAAGC
ATGGATTCAA ACTTAGGTGA AGCAGCATCT GGGTGTGAGA GTGAAACAAG CGTCTCTGAA
GACTGCTCAG GGCTATCCTC TCAGAGTGAC ATTTTAACCA CTCAGCAGAG GGATACCATG
CAACATAACC TGATAAAGCT CCAGCAGGAA ATGGCTGAAC TAGAAGCTGT GTTAGAACAG
CATGGGAGCC AGCCTTCTAA CAGCTACCCT TCCATCATAA GTGACTCTTC TGCCCTTGAG
GACCTGCGAA ATCCAGAACA AAGCACATCA GAAAAAGATT CGCATATACA TGGCCAAAGG
AACAACTCCA TGTTTTCTAA AAGGCCTAGA GAACATATAT CAGTATTAAC TTCACAGAAA
AGTAGTGAAT ACCCTATAAG CCAGAATCCA GAAGGCCTTT CTGCTGACAA GTTTGAGGTG
TCTGCAGATA GTTCTACCAG TAAAAATAAA GAACCAGGAG TGGAAAGGTC ATCCCCTTCT
AAATGCCCAT CATTAGATGA TAGGTGGTAC ATGCACAGTT GCTCTGGGAG TCTTCAGAAT
AGAAACTACC CATCTCAAGA GGAGCTCATT AAGGTTGTTG ATGTGGAGGA GCAACAGCTG
GAAGAGTCTG GGCCACACGA TTTGACGGAA ACATCTTACT TGCCAAGGCA AGATCTAGAG
GGAACCCCTT ACCTGGAATC TGGAATCAGC CTCTTCTCTG ATGACCCTGA ATCTGATCCT
TCTGAAGACA GAGCCCCAGA GTCAGCTCGT GTTGGCAACA TACCATCTTC AACCTCTGCA
TTGAAAGTTC CCCAATTGAA AGTTGCAGAA TCTGCCCAGA GTCCAGCTGC TGCTCATACT
ACTGATACTG CTGGGTATAA TGCAATGGAA GAAAGTGTGA GCAGGGAGAA GCCAGAATTG
ACAGCTTCAA CAGAAAGGGT CAACAAAAGA ATGTCCATGG TGGTGTCTGG CCTGACCCCA
GAAGAATTTA TGCTCGTGTA CAAGTTTGCC AGAAAACACC ACATCACTTT AACTAATCTA
ATTACTGAAG AGACTACTCA TGTTGTTATG AAAACAGATG CTGAGTTTGT GTGTGAACGG
ACACTGAAAT ATTTTCTAGG AATTGCGGGA GGAAAATGGG TAGTTAGCTA TTTCTGGGTG
ACCCAGTCTA TTAAAGAAAG AAAAATGCTG AATGAGCATG ATTTTGAAGT CAGAGGAGAT
GTGGTCAATG GAAGAAACCA CCAAGGTCCA AAGCGAGCAA GAGAATCCCA GGACAGAAAG
ATCTTCAGGG GGCTAGAAAT CTGTTGCTAT GGGCCCTTCA CCAACATGCC CACAGATCAA
CTGGAATGGA TGGTACAGCT GTGTGGTGCT TCTGTGGTGA AGGAGCTTTC ATCATTCACC
CTTGGCACAG GTGTCCACCC AATTGTGGTT GTGCAGCCAG ATGCCTGGAC AGAGGACAAT
GGCTTCCATG CAATTGGGCA GATGTGTGAG GCACCTGTGG TGACCCGAGA GTGGGTGTTG
GACAGTGTAG CACTCTACCA GTGCCAGGAG CTGGACACCT ACCTGATACC CCAGATCCCC
CACAGCCACT ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_009231.2
CDS233..5887
Misc Feature(1)93..93(+)
Misc Feature(2)209..211(+)
Misc Feature(3)233..235(+)
Misc Feature(4)299..436(+)
Misc Feature(5)302..424(+)
Misc Feature(6)572..574(+)
Misc Feature(7)1154..1156(+)
Misc Feature(8)1262..1753(+)
Misc Feature(9)1415..1417(+)
Misc Feature(10)1424..1426(+)
Misc Feature(11)1499..1501(+)
Misc Feature(12)2174..3166(+)
Misc Feature(13)2312..2314(+)
Misc Feature(14)2489..2491(+)
Misc Feature(15)2750..2752(+)
Misc Feature(16)3194..3196(+)
Misc Feature(17)3659..3661(+)
Misc Feature(18)3863..3865(+)
Misc Feature(19)3881..3883(+)
Misc Feature(20)3884..3886(+)
Misc Feature(21)4070..4072(+)
Misc Feature(22)4214..4216(+)
Misc Feature(23)4238..4240(+)
Misc Feature(24)4256..4258(+)
Misc Feature(25)4391..4393(+)
Misc Feature(26)4412..4414(+)
Misc Feature(27)4421..4504(+)
Misc Feature(28)4499..4501(+)
Misc Feature(29)4664..4666(+)
Misc Feature(30)4865..4867(+)
Misc Feature(31)5243..5467(+)
Misc Feature(32)5282..5320(+)
Misc Feature(33)5447..5461(+)
Misc Feature(34)5582..5821(+)
Misc Feature(35)5627..5665(+)
Misc Feature(36)5804..5818(+)
Exon (1)1..213
Gene:BRCA1
Gene Synonym:
Exon (2)214..312
Gene:BRCA1
Gene Synonym:
Exon (3)313..366
Gene:BRCA1
Gene Synonym:
Exon (4)367..444
Gene:BRCA1
Gene Synonym:
Exon (5)445..533
Gene:BRCA1
Gene Synonym:
Exon (6)534..673
Gene:BRCA1
Gene Synonym:
Exon (7)674..779
Gene:BRCA1
Gene Synonym:
Exon (8)780..825
Gene:BRCA1
Gene Synonym:
Exon (9)826..902
Gene:BRCA1
Gene Synonym:
Exon (10)903..4328
Gene:BRCA1
Gene Synonym:
Exon (11)4329..4417
Gene:BRCA1
Gene Synonym:
Exon (12)4418..4589
Gene:BRCA1
Gene Synonym:
Exon (13)4590..4655
Gene:BRCA1
Gene Synonym:
Exon (14)4656..4779
Gene:BRCA1
Gene Synonym:
Exon (15)4780..4970
Gene:BRCA1
Gene Synonym:
Exon (16)4971..5281
Gene:BRCA1
Gene Synonym:
Exon (17)5282..5369
Gene:BRCA1
Gene Synonym:
Exon (18)5370..5447
Gene:BRCA1
Gene Synonym:
Exon (19)5448..5488
Gene:BRCA1
Gene Synonym:
Exon (20)5489..5572
Gene:BRCA1
Gene Synonym:
Exon (21)5573..5627
Gene:BRCA1
Gene Synonym:
Exon (22)5628..5701
Gene:BRCA1
Gene Synonym:
Exon (23)5702..5762
Gene:BRCA1
Gene Synonym:
Exon (24)5763..7270
Gene:BRCA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_007300
Organism Homo sapiens (human)
Definition Homo sapiens breast cancer 1, early onset (BRCA1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_007300

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
ATGGATTTAT CTGCTCTTCG CGTTGAAGAA GTACAAAATG TCATTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCCATCTG TCTGGAGTTG ATCAAGGAAC CTGTCTCCAC AAAGTGTGAC
CACATATTTT GCAAATTTTG CATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAG
TGTCCTTTAT GTAAGAATGA TATAACCAAA AGGAGCCTAC AAGAAAGTAC GAGATTTAGT
CAACTTGTTG AAGAGCTATT GAAAATCATT TGTGCTTTTC AGCTTGACAC AGGTTTGGAG
TATGCAAACA GCTATAATTT TGCAAAAAAG GAAAATAACT CTCCTGAACA TCTAAAAGAT
GAAGTTTCTA TCATCCAAAG TATGGGCTAC AGAAACCGTG CCAAAAGACT TCTACAGAGT
GAACCCGAAA ATCCTTCCTT GCAGGAAACC AGTCTCAGTG TCCAACTCTC TAACCTTGGA
ACTGTGAGAA CTCTGAGGAC AAAGCAGCGG ATACAACCTC AAAAGACGTC TGTCTACATT
GAATTGGGAT CTGATTCTTC TGAAGATACC GTTAATAAGG CAACTTATTG CAGTGTGGGA
GATCAAGAAT TGTTACAAAT CACCCCTCAA GGAACCAGGG ATGAAATCAG TTTGGATTCT
GCAAAAAAGG CTGCTTGTGA ATTTTCTGAG ACGGATGTAA CAAATACTGA ACATCATCAA
CCCAGTAATA ATGATTTGAA CACCACTGAG AAGCGTGCAG CTGAGAGGCA TCCAGAAAAG
TATCAGGGTA GTTCTGTTTC AAACTTGCAT GTGGAGCCAT GTGGCACAAA TACTCATGCC
AGCTCATTAC AGCATGAGAA CAGCAGTTTA TTACTCACTA AAGACAGAAT GAATGTAGAA
AAGGCTGAAT TCTGTAATAA AAGCAAACAG CCTGGCTTAG CAAGGAGCCA ACATAACAGA
TGGGCTGGAA GTAAGGAAAC ATGTAATGAT AGGCGGACTC CCAGCACAGA AAAAAAGGTA
GATCTGAATG CTGATCCCCT GTGTGAGAGA AAAGAATGGA ATAAGCAGAA ACTGCCATGC
TCAGAGAATC CTAGAGATAC TGAAGATGTT CCTTGGATAA CACTAAATAG CAGCATTCAG
AAAGTTAATG AGTGGTTTTC CAGAAGTGAT GAACTGTTAG GTTCTGATGA CTCACATGAT
GGGGAGTCTG AATCAAATGC CAAAGTAGCT GATGTATTGG ACGTTCTAAA TGAGGTAGAT
GAATATTCTG GTTCTTCAGA GAAAATAGAC TTACTGGCCA GTGATCCTCA TGAGGCTTTA
ATATGTAAAA GTGAAAGAGT TCACTCCAAA TCAGTAGAGA GTAATATTGA AGACAAAATA
TTTGGGAAAA CCTATCGGAA GAAGGCAAGC CTCCCCAACT TAAGCCATGT AACTGAAAAT
CTAATTATAG GAGCATTTGT TACTGAGCCA CAGATAATAC AAGAGCGTCC CCTCACAAAT
AAATTAAAGC GTAAAAGGAG ACCTACATCA GGCCTTCATC CTGAGGATTT TATCAAGAAA
GCAGATTTGG CAGTTCAAAA GACTCCTGAA ATGATAAATC AGGGAACTAA CCAAACGGAG
CAGAATGGTC AAGTGATGAA TATTACTAAT AGTGGTCATG AGAATAAAAC AAAAGGTGAT
TCTATTCAGA ATGAGAAAAA TCCTAACCCA ATAGAATCAC TCGAAAAAGA ATCTGCTTTC
AAAACGAAAG CTGAACCTAT AAGCAGCAGT ATAAGCAATA TGGAACTCGA ATTAAATATC
CACAATTCAA AAGCACCTAA AAAGAATAGG CTGAGGAGGA AGTCTTCTAC CAGGCATATT
CATGCGCTTG AACTAGTAGT CAGTAGAAAT CTAAGCCCAC CTAATTGTAC TGAATTGCAA
ATTGATAGTT GTTCTAGCAG TGAAGAGATA AAGAAAAAAA AGTACAACCA AATGCCAGTC
AGGCACAGCA GAAACCTACA ACTCATGGAA GGTAAAGAAC CTGCAACTGG AGCCAAGAAG
AGTAACAAGC CAAATGAACA GACAAGTAAA AGACATGACA GCGATACTTT CCCAGAGCTG
AAGTTAACAA ATGCACCTGG TTCTTTTACT AAGTGTTCAA ATACCAGTGA ACTTAAAGAA
TTTGTCAATC CTAGCCTTCC AAGAGAAGAA AAAGAAGAGA AACTAGAAAC AGTTAAAGTG
TCTAATAATG CTGAAGACCC CAAAGATCTC ATGTTAAGTG GAGAAAGGGT TTTGCAAACT
GAAAGATCTG TAGAGAGTAG CAGTATTTCA TTGGTACCTG GTACTGATTA TGGCACTCAG
GAAAGTATCT CGTTACTGGA AGTTAGCACT CTAGGGAAGG CAAAAACAGA ACCAAATAAA
TGTGTGAGTC AGTGTGCAGC ATTTGAAAAC CCCAAGGGAC TAATTCATGG TTGTTCCAAA
GATAATAGAA ATGACACAGA AGGCTTTAAG TATCCATTGG GACATGAAGT TAACCACAGT
CGGGAAACAA GCATAGAAAT GGAAGAAAGT GAACTTGATG CTCAGTATTT GCAGAATACA
TTCAAGGTTT CAAAGCGCCA GTCATTTGCT CCGTTTTCAA ATCCAGGAAA TGCAGAAGAG
GAATGTGCAA CATTCTCTGC CCACTCTGGG TCCTTAAAGA AACAAAGTCC AAAAGTCACT
TTTGAATGTG AACAAAAGGA AGAAAATCAA GGAAAGAATG AGTCTAATAT CAAGCCTGTA
CAGACAGTTA ATATCACTGC AGGCTTTCCT GTGGTTGGTC AGAAAGATAA GCCAGTTGAT
AATGCCAAAT GTAGTATCAA AGGAGGCTCT AGGTTTTGTC TATCATCTCA GTTCAGAGGC
AACGAAACTG GACTCATTAC TCCAAATAAA CATGGACTTT TACAAAACCC ATATCGTATA
CCACCACTTT TTCCCATCAA GTCATTTGTT AAAACTAAAT GTAAGAAAAA TCTGCTAGAG
GAAAACTTTG AGGAACATTC AATGTCACCT GAAAGAGAAA TGGGAAATGA GAACATTCCA
AGTACAGTGA GCACAATTAG CCGTAATAAC ATTAGAGAAA ATGTTTTTAA AGAAGCCAGC
TCAAGCAATA TTAATGAAGT AGGTTCCAGT ACTAATGAAG TGGGCTCCAG TATTAATGAA
ATAGGTTCCA GTGATGAAAA CATTCAAGCA GAACTAGGTA GAAACAGAGG GCCAAAATTG
AATGCTATGC TTAGATTAGG GGTTTTGCAA CCTGAGGTCT ATAAACAAAG TCTTCCTGGA
AGTAATTGTA AGCATCCTGA AATAAAAAAG CAAGAATATG AAGAAGTAGT TCAGACTGTT
AATACAGATT TCTCTCCATA TCTGATTTCA GATAACTTAG AACAGCCTAT GGGAAGTAGT
CATGCATCTC AGGTTTGTTC TGAGACACCT GATGACCTGT TAGATGATGG TGAAATAAAG
GAAGATACTA GTTTTGCTGA AAATGACATT AAGGAAAGTT CTGCTGTTTT TAGCAAAAGC
GTCCAGAAAG GAGAGCTTAG CAGGAGTCCT AGCCCTTTCA CCCATACACA TTTGGCTCAG
GGTTACCGAA GAGGGGCCAA GAAATTAGAG TCCTCAGAAG AGAACTTATC TAGTGAGGAT
GAAGAGCTTC CCTGCTTCCA ACACTTGTTA TTTGGTAAAG TAAACAATAT ACCTTCTCAG
TCTACTAGGC ATAGCACCGT TGCTACCGAG TGTCTGTCTA AGAACACAGA GGAGAATTTA
TTATCATTGA AGAATAGCTT AAATGACTGC AGTAACCAGG TAATATTGGC AAAGGCATCT
CAGGAACATC ACCTTAGTGA GGAAACAAAA TGTTCTGCTA GCTTGTTTTC TTCACAGTGC
AGTGAATTGG AAGACTTGAC TGCAAATACA AACACCCAGG ATCCTTTCTT GATTGGTTCT
TCCAAACAAA TGAGGCATCA GTCTGAAAGC CAGGGAGTTG GTCTGAGTGA CAAGGAATTG
GTTTCAGATG ATGAAGAAAG AGGAACGGGC TTGGAAGAAA ATAATCAAGA AGAGCAAAGC
ATGGATTCAA ACTTAGGTGA AGCAGCATCT GGGTGTGAGA GTGAAACAAG CGTCTCTGAA
GACTGCTCAG GGCTATCCTC TCAGAGTGAC ATTTTAACCA CTCAGCAGAG GGATACCATG
CAACATAACC TGATAAAGCT CCAGCAGGAA ATGGCTGAAC TAGAAGCTGT GTTAGAACAG
CATGGGAGCC AGCCTTCTAA CAGCTACCCT TCCATCATAA GTGACTCTTC TGCCCTTGAG
GACCTGCGAA ATCCAGAACA AAGCACATCA GAAAAAGATT CGCATATACA TGGCCAAAGG
AACAACTCCA TGTTTTCTAA AAGGCCTAGA GAACATATAT CAGTATTAAC TTCACAGAAA
AGTAGTGAAT ACCCTATAAG CCAGAATCCA GAAGGCCTTT CTGCTGACAA GTTTGAGGTG
TCTGCAGATA GTTCTACCAG TAAAAATAAA GAACCAGGAG TGGAAAGGTC ATCCCCTTCT
AAATGCCCAT CATTAGATGA TAGGTGGTAC ATGCACAGTT GCTCTGGGAG TCTTCAGAAT
AGAAACTACC CATCTCAAGA GGAGCTCATT AAGGTTGTTG ATGTGGAGGA GCAACAGCTG
GAAGAGTCTG GGCCACACGA TTTGACGGAA ACATCTTACT TGCCAAGGCA AGATCTAGAG
GGAACCCCTT ACCTGGAATC TGGAATCAGC CTCTTCTCTG ATGACCCTGA ATCTGATCCT
TCTGAAGACA GAGCCCCAGA GTCAGCTCGT GTTGGCAACA TACCATCTTC AACCTCTGCA
TTGAAAGTTC CCCAATTGAA AGTTGCAGAA TCTGCCCAGA GTCCAGCTGC TGCTCATACT
ACTGATACTG CTGGGTATAA TGCAATGGAA GAAAGTGTGA GCAGGGAGAA GCCAGAATTG
ACAGCTTCAA CAGAAAGGGT CAACAAAAGA ATGTCCATGG TGGTGTCTGG CCTGACCCCA
GAAGAATTTA TGCTCGTGTA CAAGTTTGCC AGAAAACACC ACATCACTTT AACTAATCTA
ATTACTGAAG AGACTACTCA TGTTGTTATG AAAACAGATG CTGAGTTTGT GTGTGAACGG
ACACTGAAAT ATTTTCTAGG AATTGCGGGA GGAAAATGGG TAGTTAGCTA TTTCTGGGTG
ACCCAGTCTA TTAAAGAAAG AAAAATGCTG AATGAGCATG ATTTTGAAGT CAGAGGAGAT
GTGGTCAATG GAAGAAACCA CCAAGGTCCA AAGCGAGCAA GAGAATCCCA GGACAGAAAG
ATCTTCAGGG GGCTAGAAAT CTGTTGCTAT GGGCCCTTCA CCAACATGCC CACAGATCAA
CTGGAATGGA TGGTACAGCT GTGTGGTGCT TCTGTGGTGA AGGAGCTTTC ATCATTCACC
CTTGGCACAG GTGTCCACCC AATTGTGGTT GTGCAGCCAG ATGCCTGGAC AGAGGACAAT
GGCTTCCATG CAATTGGGCA GATGTGTGAG GCACCTGTGG TGACCCGAGA GTGGGTGTTG
GACAGTGTAG CACTCTACCA GTGCCAGGAG CTGGACACCT ACCTGATACC CCAGATCCCC
CACAGCCACT ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16419
Clone ID Related Accession (Same CDS sequence) NM_007298 , XM_006722040
Accession Version NM_007298.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2280bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 03-AUG-2015
Organism Homo sapiens (human)
Product breast cancer type 1 susceptibility protein isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL701927.1, U64805.1, BC072418.1, BU617173.1 and BU679389.1. On or before May 16, 2009 this sequence version replaced gi:63252881, gi:63252875. Transcript Variant: This variant (4, also known as BRCA1-delta11b) differs in the 5' UTR and uses two alternate in-frame splice sites in the central coding region, compared to variant 1. The encoded isoform (4) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U64805.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGATTTAT CTGCTCTTCG CGTTGAAGAA GTACAAAATG TCATTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCCATCTG TCTGGAGTTG ATCAAGGAAC CTGTCTCCAC AAAGTGTGAC
CACATATTTT GCAAATTTTG CATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAG
TGTCCTTTAT GTAAGAATGA TATAACCAAA AGGAGCCTAC AAGAAAGTAC GAGATTTAGT
CAACTTGTTG AAGAGCTATT GAAAATCATT TGTGCTTTTC AGCTTGACAC AGGTTTGGAG
TATGCAAACA GCTATAATTT TGCAAAAAAG GAAAATAACT CTCCTGAACA TCTAAAAGAT
GAAGTTTCTA TCATCCAAAG TATGGGCTAC AGAAACCGTG CCAAAAGACT TCTACAGAGT
GAACCCGAAA ATCCTTCCTT GCAGGAAACC AGTCTCAGTG TCCAACTCTC TAACCTTGGA
ACTGTGAGAA CTCTGAGGAC AAAGCAGCGG ATACAACCTC AAAAGACGTC TGTCTACATT
GAATTGGGAT CTGATTCTTC TGAAGATACC GTTAATAAGG CAACTTATTG CAGTGTGGGA
GATCAAGAAT TGTTACAAAT CACCCCTCAA GGAACCAGGG ATGAAATCAG TTTGGATTCT
GCAAAAAAGG CTGCTTGTGA ATTTTCTGAG ACGGATGTAA CAAATACTGA ACATCATCAA
CCCAGTAATA ATGATTTGAA CACCACTGAG AAGCGTGCAG CTGAGAGGCA TCCAGAAAAG
TATCAGGGTG AAGCAGCATC TGGGTGTGAG AGTGAAACAA GCGTCTCTGA AGACTGCTCA
GGGCTATCCT CTCAGAGTGA CATTTTAACC ACTCAGCAGA GGGATACCAT GCAACATAAC
CTGATAAAGC TCCAGCAGGA AATGGCTGAA CTAGAAGCTG TGTTAGAACA GCATGGGAGC
CAGCCTTCTA ACAGCTACCC TTCCATCATA AGTGACTCTT CTGCCCTTGA GGACCTGCGA
AATCCAGAAC AAAGCACATC AGAAAAAGTA TTAACTTCAC AGAAAAGTAG TGAATACCCT
ATAAGCCAGA ATCCAGAAGG CCTTTCTGCT GACAAGTTTG AGGTGTCTGC AGATAGTTCT
ACCAGTAAAA ATAAAGAACC AGGAGTGGAA AGGTCATCCC CTTCTAAATG CCCATCATTA
GATGATAGGT GGTACATGCA CAGTTGCTCT GGGAGTCTTC AGAATAGAAA CTACCCATCT
CAAGAGGAGC TCATTAAGGT TGTTGATGTG GAGGAGCAAC AGCTGGAAGA GTCTGGGCCA
CACGATTTGA CGGAAACATC TTACTTGCCA AGGCAAGATC TAGAGGGAAC CCCTTACCTG
GAATCTGGAA TCAGCCTCTT CTCTGATGAC CCTGAATCTG ATCCTTCTGA AGACAGAGCC
CCAGAGTCAG CTCGTGTTGG CAACATACCA TCTTCAACCT CTGCATTGAA AGTTCCCCAA
TTGAAAGTTG CAGAATCTGC CCAGAGTCCA GCTGCTGCTC ATACTACTGA TACTGCTGGG
TATAATGCAA TGGAAGAAAG TGTGAGCAGG GAGAAGCCAG AATTGACAGC TTCAACAGAA
AGGGTCAACA AAAGAATGTC CATGGTGGTG TCTGGCCTGA CCCCAGAAGA ATTTATGCTC
GTGTACAAGT TTGCCAGAAA ACACCACATC ACTTTAACTA ATCTAATTAC TGAAGAGACT
ACTCATGTTG TTATGAAAAC AGATGCTGAG TTTGTGTGTG AACGGACACT GAAATATTTT
CTAGGAATTG CGGGAGGAAA ATGGGTAGTT AGCTATTTCT GGGTGACCCA GTCTATTAAA
GAAAGAAAAA TGCTGAATGA GCATGATTTT GAAGTCAGAG GAGATGTGGT CAATGGAAGA
AACCACCAAG GTCCAAAGCG AGCAAGAGAA TCCCAGGACA GAAAGATCTT CAGGGGGCTA
GAAATCTGTT GCTATGGGCC CTTCACCAAC ATGCCCACAG ATCAACTGGA ATGGATGGTA
CAGCTGTGTG GTGCTTCTGT GGTGAAGGAG CTTTCATCAT TCACCCTTGG CACAGGTGTC
CACCCAATTG TGGTTGTGCA GCCAGATGCC TGGACAGAGG ACAATGGCTT CCATGCAATT
GGGCAGATGT GTGAGGCACC TGTGGTGACC CGAGAGTGGG TGTTGGACAG TGTAGCACTC
TACCAGTGCC AGGAGCTGGA CACCTACCTG ATACCCCAGA TCCCCCACAG CCACTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_009229.2
CDS20..2299
Misc Feature(1)86..223(+)
Misc Feature(2)89..211(+)
Misc Feature(3)1655..1879(+)
Misc Feature(4)1706..1732(+)
Misc Feature(5)1859..1873(+)
Misc Feature(6)1994..2233(+)
Misc Feature(7)2039..2077(+)
Misc Feature(8)2216..2230(+)
Exon (1)1..99
Gene:BRCA1
Gene Synonym:
Exon (2)100..153
Gene:BRCA1
Gene Synonym:
Exon (3)154..231
Gene:BRCA1
Gene Synonym:
Exon (4)232..320
Gene:BRCA1
Gene Synonym:
Exon (5)321..460
Gene:BRCA1
Gene Synonym:
Exon (6)461..566
Gene:BRCA1
Gene Synonym:
Exon (7)567..612
Gene:BRCA1
Gene Synonym:
Exon (8)613..689
Gene:BRCA1
Gene Synonym:
Exon (9)690..806
Gene:BRCA1
Gene Synonym:
Exon (10)807..895
Gene:BRCA1
Gene Synonym:
Exon (11)896..1067
Gene:BRCA1
Gene Synonym:
Exon (12)1068..1191
Gene:BRCA1
Gene Synonym:
Exon (13)1192..1382
Gene:BRCA1
Gene Synonym:
Exon (14)1383..1693
Gene:BRCA1
Gene Synonym:
Exon (15)1694..1781
Gene:BRCA1
Gene Synonym:
Exon (16)1782..1859
Gene:BRCA1
Gene Synonym:
Exon (17)1860..1900
Gene:BRCA1
Gene Synonym:
Exon (18)1901..1984
Gene:BRCA1
Gene Synonym:
Exon (19)1985..2039
Gene:BRCA1
Gene Synonym:
Exon (20)2040..2113
Gene:BRCA1
Gene Synonym:
Exon (21)2114..2174
Gene:BRCA1
Gene Synonym:
Exon (22)2175..3682
Gene:BRCA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_007298
Organism Homo sapiens (human)
Definition Homo sapiens breast cancer 1, early onset (BRCA1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_007298

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGATTTAT CTGCTCTTCG CGTTGAAGAA GTACAAAATG TCATTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCCATCTG TCTGGAGTTG ATCAAGGAAC CTGTCTCCAC AAAGTGTGAC
CACATATTTT GCAAATTTTG CATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAG
TGTCCTTTAT GTAAGAATGA TATAACCAAA AGGAGCCTAC AAGAAAGTAC GAGATTTAGT
CAACTTGTTG AAGAGCTATT GAAAATCATT TGTGCTTTTC AGCTTGACAC AGGTTTGGAG
TATGCAAACA GCTATAATTT TGCAAAAAAG GAAAATAACT CTCCTGAACA TCTAAAAGAT
GAAGTTTCTA TCATCCAAAG TATGGGCTAC AGAAACCGTG CCAAAAGACT TCTACAGAGT
GAACCCGAAA ATCCTTCCTT GCAGGAAACC AGTCTCAGTG TCCAACTCTC TAACCTTGGA
ACTGTGAGAA CTCTGAGGAC AAAGCAGCGG ATACAACCTC AAAAGACGTC TGTCTACATT
GAATTGGGAT CTGATTCTTC TGAAGATACC GTTAATAAGG CAACTTATTG CAGTGTGGGA
GATCAAGAAT TGTTACAAAT CACCCCTCAA GGAACCAGGG ATGAAATCAG TTTGGATTCT
GCAAAAAAGG CTGCTTGTGA ATTTTCTGAG ACGGATGTAA CAAATACTGA ACATCATCAA
CCCAGTAATA ATGATTTGAA CACCACTGAG AAGCGTGCAG CTGAGAGGCA TCCAGAAAAG
TATCAGGGTG AAGCAGCATC TGGGTGTGAG AGTGAAACAA GCGTCTCTGA AGACTGCTCA
GGGCTATCCT CTCAGAGTGA CATTTTAACC ACTCAGCAGA GGGATACCAT GCAACATAAC
CTGATAAAGC TCCAGCAGGA AATGGCTGAA CTAGAAGCTG TGTTAGAACA GCATGGGAGC
CAGCCTTCTA ACAGCTACCC TTCCATCATA AGTGACTCTT CTGCCCTTGA GGACCTGCGA
AATCCAGAAC AAAGCACATC AGAAAAAGTA TTAACTTCAC AGAAAAGTAG TGAATACCCT
ATAAGCCAGA ATCCAGAAGG CCTTTCTGCT GACAAGTTTG AGGTGTCTGC AGATAGTTCT
ACCAGTAAAA ATAAAGAACC AGGAGTGGAA AGGTCATCCC CTTCTAAATG CCCATCATTA
GATGATAGGT GGTACATGCA CAGTTGCTCT GGGAGTCTTC AGAATAGAAA CTACCCATCT
CAAGAGGAGC TCATTAAGGT TGTTGATGTG GAGGAGCAAC AGCTGGAAGA GTCTGGGCCA
CACGATTTGA CGGAAACATC TTACTTGCCA AGGCAAGATC TAGAGGGAAC CCCTTACCTG
GAATCTGGAA TCAGCCTCTT CTCTGATGAC CCTGAATCTG ATCCTTCTGA AGACAGAGCC
CCAGAGTCAG CTCGTGTTGG CAACATACCA TCTTCAACCT CTGCATTGAA AGTTCCCCAA
TTGAAAGTTG CAGAATCTGC CCAGAGTCCA GCTGCTGCTC ATACTACTGA TACTGCTGGG
TATAATGCAA TGGAAGAAAG TGTGAGCAGG GAGAAGCCAG AATTGACAGC TTCAACAGAA
AGGGTCAACA AAAGAATGTC CATGGTGGTG TCTGGCCTGA CCCCAGAAGA ATTTATGCTC
GTGTACAAGT TTGCCAGAAA ACACCACATC ACTTTAACTA ATCTAATTAC TGAAGAGACT
ACTCATGTTG TTATGAAAAC AGATGCTGAG TTTGTGTGTG AACGGACACT GAAATATTTT
CTAGGAATTG CGGGAGGAAA ATGGGTAGTT AGCTATTTCT GGGTGACCCA GTCTATTAAA
GAAAGAAAAA TGCTGAATGA GCATGATTTT GAAGTCAGAG GAGATGTGGT CAATGGAAGA
AACCACCAAG GTCCAAAGCG AGCAAGAGAA TCCCAGGACA GAAAGATCTT CAGGGGGCTA
GAAATCTGTT GCTATGGGCC CTTCACCAAC ATGCCCACAG ATCAACTGGA ATGGATGGTA
CAGCTGTGTG GTGCTTCTGT GGTGAAGGAG CTTTCATCAT TCACCCTTGG CACAGGTGTC
CACCCAATTG TGGTTGTGCA GCCAGATGCC TGGACAGAGG ACAATGGCTT CCATGCAATT
GGGCAGATGT GTGAGGCACC TGTGGTGACC CGAGAGTGGG TGTTGGACAG TGTAGCACTC
TACCAGTGCC AGGAGCTGGA CACCTACCTG ATACCCCAGA TCCCCCACAG CCACTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Emerging roles of BRCA1 alternative splicing
MP, Mol. Pathol. 56 (4), 191-197 (2003)
Orban TI and Olah E.


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Expression profiles of BRCA1 splice variants in asynchronous and in G1/S synchronized tumor cell lines
Biochem. Biophys. Res. Commun. 280 (1), 32-38 (2001)
Orban TI and Olah E.


book

BRCA1: a review of structure and putative functions
Dis. Markers 13 (4), 261-274 (1998)
Paterson JW.


book

Mutations and alternative splicing of the BRCA1 gene in UK breast/ovarian cancer families
Genes Chromosomes Cancer 18 (2), 102-110 (1997)
Xu CF, Chambers JA, Nicolai H, Brown MA, Hujeirat Y, Mohammed S, Hodgson S, Kelsell DP, Spurr NK, Bishop DT and Solomon E.


book

Localization of BRCA1 and a splice variant identifies the nuclear localization signal
Mol. Cell. Biol. 17 (1), 444-452 (1997)
Thakur S, Zhang HB, Peng Y, Le H, Carroll B, Ward T, Yao J, Farid LM, Couch FJ, Wilson RB and Weber BL.


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Characterization of functional messenger RNA splice variants of BRCA1 expressed in nonmalignant and tumor-derived breast cells
Cancer Res. 56 (20), 4578-4581 (1996)
Lu M, Conzen SD, Cole CN and Arrick BA.


book

Growth retardation and tumour inhibition by BRCA1
Nat. Genet. 12 (3), 298-302 (1996)
Holt JT, Thompson ME, Szabo C, Robinson-Benion C, Arteaga CL, King MC and Jensen RA.


book

Distinct transcription start sites generate two forms of BRCA1 mRNA
Hum. Mol. Genet. 4 (12), 2259-2264 (1995)
Xu CF, Brown MA, Chambers JA, Griffiths B, Nicolai H and Solomon E.


book

A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1
Science 266 (5182), 66-71 (1994)
Miki Y, Swensen J, Shattuck-Eidens D, Futreal PA, Harshman K, Tavtigian S, Liu Q, Cochran C, Bennett LM, Ding W et al.


book

BRCA1 and BRCA2 Hereditary Breast and Ovarian Cancer
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Petrucelli,N., Daly,M.B. and Feldman,G.L.


 
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