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SRY cDNA ORF clone, Homo sapiens (human)

Gene Symbol SRY
Entrez Gene ID 6736
Full Name sex determining region Y
Synonyms SRXX1, SRXY1, TDF, TDY
General protein information
Preferred Names
sex-determining region Y protein
Names
sex-determining region Y protein
sex-determining region on Y
testis-determining factor on Y
essential protein for sex determination in human males
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

Y

Yp11.3

Summary This intronless gene encodes a transcription factor that is a member of the high mobility group (HMG)-box family of DNA-binding proteins. This protein is the testis-determining factor (TDF), which initiates male sex determination. Mutations in this gene give rise to XY females with gonadal dysgenesis (Swyer syndrome); translocation of part of the Y chromosome containing this gene to the X chromosome causes XX male syndrome. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

480000

Disorder Html: 46XY complete gonadal dysgenesis, 400044 (3); 46XX true

mRNA and Protein(s)

mRNA Protein Name
NM_003140 NP_003131 sex-determining region Y protein



Homo sapiens (human) SRY NP_003131.1
Pan troglodytes (chimpanzee) SRY NP_001008988.1
Bos taurus (cattle) SRY NP_001014407.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SRY gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SRY cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu28314
NM_003140 Homo sapiens sex determining region Y (SRY), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
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30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu28314
Clone ID Related Accession (Same CDS sequence) NM_003140
Accession Version NM_003140.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 615bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 20-JUN-2014
Organism Homo sapiens (human)
Product sex-determining region Y protein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006040.3 and L10101.1. This sequence is a reference standard in the RefSeqGene project. On Jul 2, 2013 this sequence version replaced gi:4507224. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
ATGCAATCAT ATGCTTCTGC TATGTTAAGC GTATTCAACA GCGATGATTA CAGTCCAGCT 
GTGCAAGAGA ATATTCCCGC TCTCCGGAGA AGCTCTTCCT TCCTTTGCAC TGAAAGCTGT
AACTCTAAGT ATCAGTGTGA AACGGGAGAA AACAGTAAAG GCAACGTCCA GGATAGAGTG
AAGCGACCCA TGAACGCATT CATCGTGTGG TCTCGCGATC AGAGGCGCAA GATGGCTCTA
GAGAATCCCA GAATGCGAAA CTCAGAGATC AGCAAGCAGC TGGGATACCA GTGGAAAATG
CTTACTGAAG CCGAAAAATG GCCATTCTTC CAGGAGGCAC AGAAATTACA GGCCATGCAC
AGAGAGAAAT ACCCGAATTA TAAGTATCGA CCTCGTCGGA AGGCGAAGAT GCTGCCGAAG
AATTGCAGTT TGCTTCCCGC AGATCCCGCT TCGGTACTCT GCAGCGAAGT GCAACTGGAC
AACAGGTTGT ACAGGGATGA CTGTACGAAA GCCACACACT CAAGAATGGA GCACCAGCTA
GGCCACTTAC CGCCCATCAA CGCAGCCAGC TCACCGCAGC AACGGGACCG CTACAGCCAC
TGGACAAAGC TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003131.1
CDS139..753
Misc Feature(1)1..1(+)
Misc Feature(2)79..81(+)
Misc Feature(3)313..546(+)
Misc Feature(4)313..528(+)
Misc Feature(5)319..369(+)
Misc Feature(6)322..489(+)
Misc Feature(7)457..555(+)
Misc Feature(8)526..546(+)
Misc Feature(9)544..546(+)
Misc Feature(10)550..603(+)
Misc Feature(11)730..750(+)
Exon (1)1..887
Gene:SRY
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_003140
Organism Homo sapiens (human)
Definition Homo sapiens sex determining region Y (SRY), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003140

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGCAATCAT ATGCTTCTGC TATGTTAAGC GTATTCAACA GCGATGATTA CAGTCCAGCT 
GTGCAAGAGA ATATTCCCGC TCTCCGGAGA AGCTCTTCCT TCCTTTGCAC TGAAAGCTGT
AACTCTAAGT ATCAGTGTGA AACGGGAGAA AACAGTAAAG GCAACGTCCA GGATAGAGTG
AAGCGACCCA TGAACGCATT CATCGTGTGG TCTCGCGATC AGAGGCGCAA GATGGCTCTA
GAGAATCCCA GAATGCGAAA CTCAGAGATC AGCAAGCAGC TGGGATACCA GTGGAAAATG
CTTACTGAAG CCGAAAAATG GCCATTCTTC CAGGAGGCAC AGAAATTACA GGCCATGCAC
AGAGAGAAAT ACCCGAATTA TAAGTATCGA CCTCGTCGGA AGGCGAAGAT GCTGCCGAAG
AATTGCAGTT TGCTTCCCGC AGATCCCGCT TCGGTACTCT GCAGCGAAGT GCAACTGGAC
AACAGGTTGT ACAGGGATGA CTGTACGAAA GCCACACACT CAAGAATGGA GCACCAGCTA
GGCCACTTAC CGCCCATCAA CGCAGCCAGC TCACCGCAGC AACGGGACCG CTACAGCCAC
TGGACAAAGC TGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

MAS promoter regulation: a role for Sry and tyrosine nitration of the KRAB domain of ZNF274 as a feedback mechanism
Clin. Sci. 126 (10), 727-738 (2014)
Prokop JW, Rauscher FJ 3rd, Peng H, Liu Y, Araujo FC, Watanabe I, Reis FM and Milsted A.


book

Upstream open reading frames cause widespread reduction of protein expression and are polymorphic among humans
Proc. Natl. Acad. Sci. U.S.A. 106 (18), 7507-7512 (2009)
Calvo SE, Pagliarini DJ and Mootha VK.


book

Sex determination and SRY: down to a wink and a nudge?
Trends Genet. 25 (1), 19-29 (2009)
Sekido R and Lovell-Badge R.


book

The human SRY transcript
Hum. Mol. Genet. 2 (12), 2007-2012 (1993)
Clepet C, Schafer AJ, Sinclair AH, Palmer MS, Lovell-Badge R and Goodfellow PN.


book

Evidence that the SRY protein is encoded by a single exon on the human Y chromosome
Genomics 17 (3), 736-739 (1993)
Behlke MA, Bogan JS, Beer-Romero P and Page DC.


book

Identification of the transcriptional unit, structural organization, and promoter sequence of the human sex-determining region Y (SRY) gene, using a reverse genetic approach
Am. J. Hum. Genet. 52 (1), 24-38 (1993)
Su H and Lau YF.


book

46,XY Disorder of Sex Development and 46,XY Complete Gonadal Dysgenesis
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Ostrer,H.


book

46,XX Testicular Disorder of Sex Development
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Vilain,E.J.


book

Mutational analysis of SRY: nonsense and missense mutations in XY sex reversal
Hum. Genet. 88 (4), 471-474 (1992)
Hawkins JR, Taylor A, Berta P, Levilliers J, Van der Auwera B and Goodfellow PN.


book

A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif
Nature 346 (6281), 240-244 (1990)
Sinclair AH, Berta P, Palmer MS, Hawkins JR, Griffiths BL, Smith MJ, Foster JW, Frischauf AM, Lovell-Badge R and Goodfellow PN.


 
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