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T T brachyury transcription factor [Homo sapiens (human)]

Gene Symbol T
Entrez Gene ID 6862
Full Name T brachyury transcription factor
Synonyms SAVA, TFT
General protein information
Preferred Names
brachyury protein
Names
brachyury protein
protein T
T brachyury homolog
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6q27

Summary The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]. lac of sum
Disorder MIM:

601397

Disorder Html: {Neural tube defects, susceptibility to}, 182940 (3)
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu60837 XM_011536080 PREDICTED: Homo sapiens T, brachyury homolog (mouse) (T), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu17747 XM_011536081 PREDICTED: Homo sapiens T, brachyury homolog (mouse) (T), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu17741 NM_003181 Homo sapiens T brachyury transcription factor (T), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OHu17747 NM_001270484 Homo sapiens T brachyury transcription factor (T), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu60837D
Sequence Information ORF Nucleotide Sequence (Length: 1311bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product brachyury protein isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_025741.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG
TACTCCCAAT CAGGGGGGTG GCTTCTTCCT GGAACCAGCA CCCTGTGTCC ACCTGCAAAT
CCTCATCCTC AGTTTGGAGG TGCCCTCTCC CTCCCCTCCA CGCACAGCTG TGACAGGTAC
CCAACCCTGA GGAGCCACCG GTCCTCACCC TACCCCAGCC CCTATGCTCA TCGGAACAAT
TCTCCAACCT ATTCTGACAA CTCACCTGCA TGTTTATCCA TGCTGCAATC CCATGACAAT
TGGTCCAGCC TTGGAATGCC TGCCCATCCC AGCATGCTCC CCGTGAGCCA CAATGCCAGC
CCACCTACCA GCTCCAGTCA GTACCCCAGC CTGTGGTCTG TGAGCAACGG CGCCGTCACC
CCGGGCTCCC AGGCAGCAGC CGTGTCCAAC GGGCTGGGGG CCCAGTTCTT CCGGGGCTCC
CCCGCGCACT ACACACCCCT CACCCATCCG GTCTCGGCGC CCTCTTCCTC GGGATCCCCA
CTGTACGAAG GGGCGGCCGC GGCCACAGAC ATCGTGGACA GCCAGTACGA CGCCGCAGCC
CAAGGCCGCC TCATAGCCTC ATGGACACCT GTGTCGCCAC CTTCCATGTTG A
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011534382.1
CDS287..1597
Misc Feature(1)410..955(+)
Misc Feature(2)473..943(+)
Misc Feature(3)545..811(+)
Translation
Position Chain Variation Link
21 21 a, g dbSNP:539496719
30 30 a, g dbSNP:569326937
33 33 c, t dbSNP:550696843
43 43 a, g dbSNP:535252784
53 53 c, g dbSNP:567888404
92 92 c, t dbSNP:757609097
152 152 g, t dbSNP:546408427
175 175 c, t dbSNP:112553716
186 186 c, g dbSNP:528339157
219 219 a, g dbSNP:3099266
246 246 c, g dbSNP:755025433
257 257 c, g dbSNP:754396517
263 263 c, t dbSNP:766904827
266 266 a, g dbSNP:756439333
268 268 a, c dbSNP:750740007
271 271 a, g dbSNP:3734509
274 274 a, g dbSNP:761519813
277 277 a, g dbSNP:187193467
280 280 a, c dbSNP:763746928
283 283 -, g dbSNP:763672068
304 304 c, t dbSNP:762539762
305 305 c, g dbSNP:775360515
308 308 a, t dbSNP:180733987
315 315 a, c, g dbSNP:745665800
319 319 c, g dbSNP:776440268
331 331 c, t dbSNP:770680343
332 332 a, c dbSNP:748415738
335 335 a, g dbSNP:779090198
352 352 a, c dbSNP:534228470
354 354 c, t dbSNP:778891218
356 356 a, c, g dbSNP:563349798
370 370 g, t dbSNP:780438912
376 376 g, t dbSNP:756594225
377 377 a, g dbSNP:750783022
380 380 a, g dbSNP:150278117
400 400 a, g dbSNP:757676401
401 401 a, g dbSNP:760479099
402 402 a, t dbSNP:751315759
404 404 c, g dbSNP:763835047
407 407 g, t dbSNP:762562087
410 410 a, c, g dbSNP:141082493
413 413 a, c dbSNP:370204149
425 425 a, g dbSNP:776406514
427 427 a, g dbSNP:770766611
431 431 a, t dbSNP:760455239
433 433 a, c dbSNP:774559741
447 447 g, t dbSNP:377517710
455 455 a, g dbSNP:768807770
460 460 a, c dbSNP:749347520
476 476 g, t dbSNP:780139385
484 484 a, g dbSNP:769733462
513 513 a, g dbSNP:754517228
515 515 g, t dbSNP:753229275
535 535 c, g dbSNP:370830801
546 546 c, t dbSNP:376160217
563 563 c, g dbSNP:373492906
571 571 g, t dbSNP:756004401
574 574 a, g dbSNP:750329241
580 580 c, t dbSNP:920961
586 586 c, g dbSNP:761502724
588 588 g, t dbSNP:775848412
598 598 a, c, t dbSNP:759679493
602 602 a, g dbSNP:776807169
605 605 c, g dbSNP:771599771
613 613 g, t dbSNP:747562377
617 617 c, t dbSNP:773839438
618 618 c, t dbSNP:772551453
619 619 a, c, g dbSNP:778637353
622 622 c, g dbSNP:200980847
624 624 -, g dbSNP:757779131
630 630 c, t dbSNP:754536703
644 644 c, t dbSNP:201323620
648 648 g, t dbSNP:374750210
649 649 c, t dbSNP:1056048
652 652 c, t dbSNP:201970663
667 667 c, t dbSNP:767353156
670 670 a, c, t dbSNP:751255909
685 685 a, g dbSNP:373205880
686 686 a, g dbSNP:759641403
688 688 c, t dbSNP:776964790
701 701 g, t dbSNP:766618894
705 705 c, t dbSNP:368969464
706 706 c, t dbSNP:376242587
707 707 a, g dbSNP:371635418
746 746 c, g dbSNP:772397551
751 751 c, g dbSNP:748566677
754 754 a, c, t dbSNP:368328281
760 760 c, g dbSNP:368405992
769 769 c, t dbSNP:763305561
772 772 c, t dbSNP:775129967
785 785 a, g dbSNP:546389815
787 787 a, g dbSNP:376732326
798 798 a, g dbSNP:587777303
809 809 a, g dbSNP:780531257
816 816 a, g dbSNP:2305089
822 822 a, g dbSNP:746971152
825 825 a, c, g dbSNP:758269436
830 830 a, c dbSNP:752478625
831 831 c, t dbSNP:780442577
833 833 a, c dbSNP:201970019
839 839 a, c dbSNP:750627725
856 856 c, t dbSNP:541803907
857 857 c, g dbSNP:761937886
858 858 a, g dbSNP:752074396
866 866 g, t dbSNP:764519598
868 868 a, g dbSNP:763321958
886 886 c, t dbSNP:374566002
915 915 a, t dbSNP:748143125
956 956 a, c dbSNP:758453282
959 959 g, t dbSNP:571236975
962 962 c, t dbSNP:774760380
966 966 a, t dbSNP:755308093
984 984 c, g dbSNP:754178278
985 985 c, t dbSNP:141018360
986 986 a, g dbSNP:537568755
989 989 a, g dbSNP:374862419
1001 1001 c, t dbSNP:370348152
1012 1012 c, g dbSNP:761403739
1016 1016 c, t dbSNP:774434198
1017 1017 a, c dbSNP:762990244
1019 1019 a, g dbSNP:775270433
1020 1020 a, g dbSNP:769691133
1021 1021 c, g dbSNP:759325329
1023 1023 c, g dbSNP:369452447
1049 1049 c, g dbSNP:3127336
1051 1051 a, g dbSNP:772229729
1053 1053 g, t dbSNP:748254170
1057 1057 a, t dbSNP:539561632
1058 1058 c, t dbSNP:768592312
1059 1059 c, g dbSNP:749862609
1067 1067 c, t dbSNP:200268852
1068 1068 c, t dbSNP:756506255
1072 1072 c, t dbSNP:550364175
1078 1078 a, g dbSNP:781015050
1086 1086 a, g, t dbSNP:373379157
1091 1091 c, t dbSNP:751227884
1107 1107 c, t dbSNP:35694129
1108 1108 a, g dbSNP:369239526
1123 1123 g, t dbSNP:752749538
1125 1125 a, g dbSNP:139829897
1127 1127 c, t dbSNP:759343495
1130 1130 a, g dbSNP:776366565
1139 1139 a, c dbSNP:770678347
1140 1140 a, g dbSNP:762068341
1145 1145 a, c, t dbSNP:768845167
1146 1146 a, g dbSNP:527987217
1150 1150 a, c dbSNP:776077161
1170 1170 a, g dbSNP:770375711
1178 1178 c, t dbSNP:746266432
1179 1179 a, g dbSNP:781720077
1184 1184 a, t dbSNP:757674783
1189 1189 a, t dbSNP:746736950
1197 1197 a, g, t dbSNP:748045953
1214 1214 a, g dbSNP:369534625
1217 1217 c, t dbSNP:755916571
1224 1224 a, c dbSNP:143862444
1225 1225 c, t dbSNP:541928116
1226 1226 a, g, t dbSNP:376805428
1234 1234 a, g dbSNP:199515427
1243 1243 c, t dbSNP:775760510
1245 1245 a, g dbSNP:552884807
1249 1249 -, aa dbSNP:781706833
1249 1249 g, t dbSNP:759687323
1250 1250 -, g dbSNP:771364595
1250 1250 g, t dbSNP:777285628
1251 1251 c, t dbSNP:771510108
1252 1252 c, t dbSNP:267600890
1253 1253 a, t dbSNP:761128287
1255 1255 a, c dbSNP:773484640
1264 1264 a, g dbSNP:772457578
1266 1266 c, t dbSNP:747810651
1271 1271 -, c dbSNP:746662856
1278 1278 c, g, t dbSNP:375777542
1281 1281 c, t dbSNP:748736752
1288 1288 c, g, t dbSNP:116620192
1289 1289 a, g dbSNP:768390079
1302 1302 c, t dbSNP:117097130
1303 1303 a, c dbSNP:757063703
1305 1305 a, g dbSNP:779700774
1315 1315 c, t dbSNP:200600273
1317 1317 a, g dbSNP:372221322
1332 1332 c, t dbSNP:766423451
1333 1333 c, t dbSNP:756211870
1354 1354 c, t dbSNP:200879575
1355 1355 a, g dbSNP:3127328
1357 1357 c, t dbSNP:762185412
1359 1359 c, t dbSNP:774657641
1360 1360 c, t dbSNP:764411092
1361 1361 a, g dbSNP:77703807
1366 1366 c, t dbSNP:775111459
1368 1368 c, t dbSNP:769256401
1369 1369 a, g dbSNP:745320047
1379 1379 c, g dbSNP:776449248
1388 1388 a, g dbSNP:35292451
1395 1395 a, g dbSNP:3816300
1396 1396 c, t dbSNP:369470652
1397 1397 a, g, t dbSNP:749584982
1399 1399 a, g dbSNP:780253490
1401 1401 c, t dbSNP:375108148
1405 1405 a, g dbSNP:561656601
1406 1406 g, t dbSNP:750466703
1410 1410 a, c dbSNP:147494273
1418 1418 c, t dbSNP:143094140
1419 1419 a, g dbSNP:368179445
1422 1422 g, t dbSNP:764501069
1429 1429 c, t dbSNP:763303937
1430 1430 a, g dbSNP:775012230
1431 1431 c, t dbSNP:764888773
1432 1432 a, g, t dbSNP:776170985
1433 1433 c, t dbSNP:573464240
1436 1436 c, t dbSNP:770321070
1438 1438 c, t dbSNP:746996355
1443 1443 c, t dbSNP:773275146
1447 1447 c, t dbSNP:543190873
1451 1451 c, t dbSNP:747724104
1452 1452 a, g dbSNP:139057807
1454 1454 a, c dbSNP:780160101
1455 1455 c, t dbSNP:756318370
1456 1456 a, g dbSNP:143219390
1461 1461 a, c dbSNP:149220881
1463 1463 g, t dbSNP:138300044
1464 1464 c, t dbSNP:373982301
1465 1465 a, g dbSNP:35819705
1468 1468 c, g dbSNP:778247718
1469 1469 g, t dbSNP:758818312
1476 1476 c, t dbSNP:182159312
1477 1477 a, g dbSNP:764835063
1487 1487 c, g dbSNP:759260639
1488 1488 c, t dbSNP:376697758
1492 1492 c, t dbSNP:765996548
1493 1493 a, g dbSNP:34517945
1498 1498 a, g dbSNP:773343096
1500 1500 a, c, t dbSNP:761554301
1504 1504 c, t dbSNP:201872052
1506 1506 c, t dbSNP:537883499
1509 1509 c, g dbSNP:746115753
1512 1512 -, ggccac dbSNP:777285210
1519 1519 c, t dbSNP:781204071
1520 1520 a, g dbSNP:771064715
1525 1525 c, t dbSNP:747076395
1527 1527 a, g dbSNP:567735651
1534 1534 c, t dbSNP:375588439
1535 1535 a, g dbSNP:753100833
1537 1537 c, t dbSNP:370987280
1538 1538 a, g dbSNP:755234783
1540 1540 c, t dbSNP:372419736
1541 1541 a, g dbSNP:766120230
1545 1545 c, t dbSNP:760313386
1547 1547 c, t dbSNP:749923447
1553 1553 c, t dbSNP:767098205
1554 1554 a, g dbSNP:761833646
1560 1560 c, t dbSNP:146841649
1566 1566 a, c dbSNP:768501878
1570 1570 a, g dbSNP:144704487
1574 1574 c, t dbSNP:776894476
1578 1578 g, t dbSNP:771117935
1581 1581 c, t dbSNP:372990504
1582 1582 a, g dbSNP:140876874
1585 1585 a, t dbSNP:772086874
1586 1586 c, t dbSNP:181394470
1587 1587 c, g dbSNP:748697550
1592 1592 a, g dbSNP:779287427
1603 1603 c, g dbSNP:755431891
1615 1615 a, c dbSNP:368784999
1617 1617 c, t dbSNP:377596495
1618 1618 a, g dbSNP:373627873
1627 1627 -, a dbSNP:757977861
1629 1629 c, t dbSNP:199931571
1638 1638 c, g, t dbSNP:756655657
1640 1640 c, t dbSNP:751580961
1641 1641 a, g dbSNP:200998218
1643 1643 a, g dbSNP:762853565
1644 1644 c, g dbSNP:775232029
1645 1645 c, g dbSNP:764968975
1675 1675 a, g dbSNP:550527863
1721 1721 a, t dbSNP:528914986
1726 1726 c, t dbSNP:561738872
1735 1735 a, g dbSNP:539921601
1751 1751 c, t dbSNP:79882341
1781 1781 a, t dbSNP:560625615
1790 1790 a, c dbSNP:778091431
1800 1800 c, t dbSNP:1056050
1821 1821 a, c dbSNP:1056051
1826 1826 g, t dbSNP:556932718
1831 1831 c, t dbSNP:565758718
1847 1847 c, t dbSNP:3816302
1870 1870 c, t dbSNP:35606910
1875 1875 a, g dbSNP:3099270
1927 1927 c, t dbSNP:533997416
1936 1936 a, g dbSNP:3816304
1938 1938 a, c dbSNP:756117325
1957 1957 a, g dbSNP:1056053
1972 1972 a, c dbSNP:767203205
1982 1982 a, t dbSNP:761167980
2041 2041 a, g dbSNP:539277276
2068 2068 c, t dbSNP:773897685
2114 2114 c, t dbSNP:112492682
2115 2115 c, g dbSNP:568794612
2120 2120 g, t dbSNP:3127327
2139 2139 a, c dbSNP:550567543
2142 2142 c, t dbSNP:768196150
2154 2154 c, t dbSNP:529208108
2217 2217 g, t dbSNP:746353519
2236 2236 a, c dbSNP:1134481
2243 2243 c, t dbSNP:1134482
2254 2254 a, g dbSNP:747408295

Target ORF information:

RefSeq Version XM_011536080
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens T, brachyury homolog (mouse) (T), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011536080

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG
TACTCCCAAT CAGGGGGGTG GCTTCTTCCT GGAACCAGCA CCCTGTGTCC ACCTGCAAAT
CCTCATCCTC AGTTTGGAGG TGCCCTCTCC CTCCCCTCCA CGCACAGCTG TGACAGGTAC
CCAACCCTGA GGAGCCACCG GTCCTCACCC TACCCCAGCC CCTATGCTCA TCGGAACAAT
TCTCCAACCT ATTCTGACAA CTCACCTGCA TGTTTATCCA TGCTGCAATC CCATGACAAT
TGGTCCAGCC TTGGAATGCC TGCCCATCCC AGCATGCTCC CCGTGAGCCA CAATGCCAGC
CCACCTACCA GCTCCAGTCA GTACCCCAGC CTGTGGTCTG TGAGCAACGG CGCCGTCACC
CCGGGCTCCC AGGCAGCAGC CGTGTCCAAC GGGCTGGGGG CCCAGTTCTT CCGGGGCTCC
CCCGCGCACT ACACACCCCT CACCCATCCG GTCTCGGCGC CCTCTTCCTC GGGATCCCCA
CTGTACGAAG GGGCGGCCGC GGCCACAGAC ATCGTGGACA GCCAGTACGA CGCCGCAGCC
CAAGGCCGCC TCATAGCCTC ATGGACACCT GTGTCGCCAC CTTCCATGTTG A

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu17747D
Sequence Information ORF Nucleotide Sequence (Length: 1134bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product brachyury protein isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_025741.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG
TACTCCCAAT CCTATTCTGA CAACTCACCT GCATGTTTAT CCATGCTGCA ATCCCATGAC
AATTGGTCCA GCCTTGGAAT GCCTGCCCAT CCCAGCATGC TCCCCGTGAG CCACAATGCC
AGCCCACCTA CCAGCTCCAG TCAGTACCCC AGCCTGTGGT CTGTGAGCAA CGGCGCCGTC
ACCCCGGGCT CCCAGGCAGC AGCCGTGTCC AACGGGCTGG GGGCCCAGTT CTTCCGGGGC
TCCCCCGCGC ACTACACACC CCTCACCCAT CCGGTCTCGG CGCCCTCTTC CTCGGGATCC
CCACTGTACG AAGGGGCGGC CGCGGCCACA GACATCGTGG ACAGCCAGTA CGACGCCGCA
GCCCAAGGCC GCCTCATAGC CTCATGGACA CCTGTGTCGC CACCTTCCAT GTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011534383.1
CDS153..1286
Misc Feature(1)276..821(+)
Misc Feature(2)339..809(+)
Misc Feature(3)411..677(+)
Misc Feature(4)663..989(+)
Translation
Position Chain Variation Link
18 18 g, t dbSNP:546408427
41 41 c, t dbSNP:112553716
52 52 c, g dbSNP:528339157
85 85 a, g dbSNP:3099266
112 112 c, g dbSNP:755025433
123 123 c, g dbSNP:754396517
129 129 c, t dbSNP:766904827
132 132 a, g dbSNP:756439333
134 134 a, c dbSNP:750740007
137 137 a, g dbSNP:3734509
140 140 a, g dbSNP:761519813
143 143 a, g dbSNP:187193467
146 146 a, c dbSNP:763746928
149 149 -, g dbSNP:763672068
170 170 c, t dbSNP:762539762
171 171 c, g dbSNP:775360515
174 174 a, t dbSNP:180733987
181 181 a, c, g dbSNP:745665800
185 185 c, g dbSNP:776440268
197 197 c, t dbSNP:770680343
198 198 a, c dbSNP:748415738
201 201 a, g dbSNP:779090198
218 218 a, c dbSNP:534228470
220 220 c, t dbSNP:778891218
222 222 a, c, g dbSNP:563349798
236 236 g, t dbSNP:780438912
242 242 g, t dbSNP:756594225
243 243 a, g dbSNP:750783022
246 246 a, g dbSNP:150278117
266 266 a, g dbSNP:757676401
267 267 a, g dbSNP:760479099
268 268 a, t dbSNP:751315759
270 270 c, g dbSNP:763835047
273 273 g, t dbSNP:762562087
276 276 a, c, g dbSNP:141082493
279 279 a, c dbSNP:370204149
291 291 a, g dbSNP:776406514
293 293 a, g dbSNP:770766611
297 297 a, t dbSNP:760455239
299 299 a, c dbSNP:774559741
313 313 g, t dbSNP:377517710
321 321 a, g dbSNP:768807770
326 326 a, c dbSNP:749347520
342 342 g, t dbSNP:780139385
350 350 a, g dbSNP:769733462
379 379 a, g dbSNP:754517228
381 381 g, t dbSNP:753229275
401 401 c, g dbSNP:370830801
412 412 c, t dbSNP:376160217
429 429 c, g dbSNP:373492906
437 437 g, t dbSNP:756004401
440 440 a, g dbSNP:750329241
446 446 c, t dbSNP:920961
452 452 c, g dbSNP:761502724
454 454 g, t dbSNP:775848412
464 464 a, c, t dbSNP:759679493
468 468 a, g dbSNP:776807169
471 471 c, g dbSNP:771599771
479 479 g, t dbSNP:747562377
483 483 c, t dbSNP:773839438
484 484 c, t dbSNP:772551453
485 485 a, c, g dbSNP:778637353
488 488 c, g dbSNP:200980847
490 490 -, g dbSNP:757779131
496 496 c, t dbSNP:754536703
510 510 c, t dbSNP:201323620
514 514 g, t dbSNP:374750210
515 515 c, t dbSNP:1056048
518 518 c, t dbSNP:201970663
533 533 c, t dbSNP:767353156
536 536 a, c, t dbSNP:751255909
551 551 a, g dbSNP:373205880
552 552 a, g dbSNP:759641403
554 554 c, t dbSNP:776964790
567 567 g, t dbSNP:766618894
571 571 c, t dbSNP:368969464
572 572 c, t dbSNP:376242587
573 573 a, g dbSNP:371635418
612 612 c, g dbSNP:772397551
617 617 c, g dbSNP:748566677
620 620 a, c, t dbSNP:368328281
626 626 c, g dbSNP:368405992
635 635 c, t dbSNP:763305561
638 638 c, t dbSNP:775129967
651 651 a, g dbSNP:546389815
653 653 a, g dbSNP:376732326
664 664 a, g dbSNP:587777303
675 675 a, g dbSNP:780531257
682 682 a, g dbSNP:2305089
688 688 a, g dbSNP:746971152
691 691 a, c, g dbSNP:758269436
696 696 a, c dbSNP:752478625
697 697 c, t dbSNP:780442577
699 699 a, c dbSNP:201970019
705 705 a, c dbSNP:750627725
722 722 c, t dbSNP:541803907
723 723 c, g dbSNP:761937886
724 724 a, g dbSNP:752074396
732 732 g, t dbSNP:764519598
734 734 a, g dbSNP:763321958
752 752 c, t dbSNP:374566002
781 781 a, t dbSNP:748143125
822 822 a, c dbSNP:758453282
825 825 g, t dbSNP:571236975
828 828 c, t dbSNP:774760380
832 832 a, t dbSNP:755308093
850 850 c, g dbSNP:754178278
851 851 c, t dbSNP:141018360
852 852 a, g dbSNP:537568755
855 855 a, g dbSNP:374862419
867 867 c, t dbSNP:370348152
878 878 c, g dbSNP:761403739
882 882 c, t dbSNP:774434198
886 886 a, g, t dbSNP:748045953
903 903 a, g dbSNP:369534625
906 906 c, t dbSNP:755916571
913 913 a, c dbSNP:143862444
914 914 c, t dbSNP:541928116
915 915 a, g, t dbSNP:376805428
923 923 a, g dbSNP:199515427
932 932 c, t dbSNP:775760510
934 934 a, g dbSNP:552884807
938 938 -, aa dbSNP:781706833
938 938 g, t dbSNP:759687323
939 939 -, g dbSNP:771364595
939 939 g, t dbSNP:777285628
940 940 c, t dbSNP:771510108
941 941 c, t dbSNP:267600890
942 942 a, t dbSNP:761128287
944 944 a, c dbSNP:773484640
953 953 a, g dbSNP:772457578
955 955 c, t dbSNP:747810651
960 960 -, c dbSNP:746662856
967 967 c, g, t dbSNP:375777542
970 970 c, t dbSNP:748736752
977 977 c, g, t dbSNP:116620192
978 978 a, g dbSNP:768390079
991 991 c, t dbSNP:117097130
992 992 a, c dbSNP:757063703
994 994 a, g dbSNP:779700774
1004 1004 c, t dbSNP:200600273
1006 1006 a, g dbSNP:372221322
1021 1021 c, t dbSNP:766423451
1022 1022 c, t dbSNP:756211870
1043 1043 c, t dbSNP:200879575
1044 1044 a, g dbSNP:3127328
1046 1046 c, t dbSNP:762185412
1048 1048 c, t dbSNP:774657641
1049 1049 c, t dbSNP:764411092
1050 1050 a, g dbSNP:77703807
1055 1055 c, t dbSNP:775111459
1057 1057 c, t dbSNP:769256401
1058 1058 a, g dbSNP:745320047
1068 1068 c, g dbSNP:776449248
1077 1077 a, g dbSNP:35292451
1084 1084 a, g dbSNP:3816300
1085 1085 c, t dbSNP:369470652
1086 1086 a, g, t dbSNP:749584982
1088 1088 a, g dbSNP:780253490
1090 1090 c, t dbSNP:375108148
1094 1094 a, g dbSNP:561656601
1095 1095 g, t dbSNP:750466703
1099 1099 a, c dbSNP:147494273
1107 1107 c, t dbSNP:143094140
1108 1108 a, g dbSNP:368179445
1111 1111 g, t dbSNP:764501069
1118 1118 c, t dbSNP:763303937
1119 1119 a, g dbSNP:775012230
1120 1120 c, t dbSNP:764888773
1121 1121 a, g, t dbSNP:776170985
1122 1122 c, t dbSNP:573464240
1125 1125 c, t dbSNP:770321070
1127 1127 c, t dbSNP:746996355
1132 1132 c, t dbSNP:773275146
1136 1136 c, t dbSNP:543190873
1140 1140 c, t dbSNP:747724104
1141 1141 a, g dbSNP:139057807
1143 1143 a, c dbSNP:780160101
1144 1144 c, t dbSNP:756318370
1145 1145 a, g dbSNP:143219390
1150 1150 a, c dbSNP:149220881
1152 1152 g, t dbSNP:138300044
1153 1153 c, t dbSNP:373982301
1154 1154 a, g dbSNP:35819705
1157 1157 c, g dbSNP:778247718
1158 1158 g, t dbSNP:758818312
1165 1165 c, t dbSNP:182159312
1166 1166 a, g dbSNP:764835063
1176 1176 c, g dbSNP:759260639
1177 1177 c, t dbSNP:376697758
1181 1181 c, t dbSNP:765996548
1182 1182 a, g dbSNP:34517945
1187 1187 a, g dbSNP:773343096
1189 1189 a, c, t dbSNP:761554301
1193 1193 c, t dbSNP:201872052
1195 1195 c, t dbSNP:537883499
1198 1198 c, g dbSNP:746115753
1201 1201 -, ggccac dbSNP:777285210
1208 1208 c, t dbSNP:781204071
1209 1209 a, g dbSNP:771064715
1214 1214 c, t dbSNP:747076395
1216 1216 a, g dbSNP:567735651
1223 1223 c, t dbSNP:375588439
1224 1224 a, g dbSNP:753100833
1226 1226 c, t dbSNP:370987280
1227 1227 a, g dbSNP:755234783
1229 1229 c, t dbSNP:372419736
1230 1230 a, g dbSNP:766120230
1234 1234 c, t dbSNP:760313386
1236 1236 c, t dbSNP:749923447
1242 1242 c, t dbSNP:767098205
1243 1243 a, g dbSNP:761833646
1249 1249 c, t dbSNP:146841649
1255 1255 a, c dbSNP:768501878
1259 1259 a, g dbSNP:144704487
1263 1263 c, t dbSNP:776894476
1267 1267 g, t dbSNP:771117935
1270 1270 c, t dbSNP:372990504
1271 1271 a, g dbSNP:140876874
1274 1274 a, t dbSNP:772086874
1275 1275 c, t dbSNP:181394470
1276 1276 c, g dbSNP:748697550
1281 1281 a, g dbSNP:779287427
1292 1292 c, g dbSNP:755431891
1304 1304 a, c dbSNP:368784999
1306 1306 c, t dbSNP:377596495
1307 1307 a, g dbSNP:373627873
1316 1316 -, a dbSNP:757977861
1318 1318 c, t dbSNP:199931571
1327 1327 c, g, t dbSNP:756655657
1329 1329 c, t dbSNP:751580961
1330 1330 a, g dbSNP:200998218
1332 1332 a, g dbSNP:762853565
1333 1333 c, g dbSNP:775232029
1334 1334 c, g dbSNP:764968975
1364 1364 a, g dbSNP:550527863
1410 1410 a, t dbSNP:528914986
1415 1415 c, t dbSNP:561738872
1424 1424 a, g dbSNP:539921601
1440 1440 c, t dbSNP:79882341
1470 1470 a, t dbSNP:560625615
1479 1479 a, c dbSNP:778091431
1489 1489 c, t dbSNP:1056050
1510 1510 a, c dbSNP:1056051
1515 1515 g, t dbSNP:556932718
1520 1520 c, t dbSNP:565758718
1536 1536 c, t dbSNP:3816302
1559 1559 c, t dbSNP:35606910
1564 1564 a, g dbSNP:3099270
1616 1616 c, t dbSNP:533997416
1625 1625 a, g dbSNP:3816304
1627 1627 a, c dbSNP:756117325
1646 1646 a, g dbSNP:1056053
1661 1661 a, c dbSNP:767203205
1671 1671 a, t dbSNP:761167980
1730 1730 a, g dbSNP:539277276
1757 1757 c, t dbSNP:773897685
1803 1803 c, t dbSNP:112492682
1804 1804 c, g dbSNP:568794612
1809 1809 g, t dbSNP:3127327
1828 1828 a, c dbSNP:550567543
1831 1831 c, t dbSNP:768196150
1843 1843 c, t dbSNP:529208108
1906 1906 g, t dbSNP:746353519
1925 1925 a, c dbSNP:1134481
1932 1932 c, t dbSNP:1134482
1943 1943 a, g dbSNP:747408295

Target ORF information:

RefSeq Version XM_011536081
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens T, brachyury homolog (mouse) (T), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011536081

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG
TACTCCCAAT CCTATTCTGA CAACTCACCT GCATGTTTAT CCATGCTGCA ATCCCATGAC
AATTGGTCCA GCCTTGGAAT GCCTGCCCAT CCCAGCATGC TCCCCGTGAG CCACAATGCC
AGCCCACCTA CCAGCTCCAG TCAGTACCCC AGCCTGTGGT CTGTGAGCAA CGGCGCCGTC
ACCCCGGGCT CCCAGGCAGC AGCCGTGTCC AACGGGCTGG GGGCCCAGTT CTTCCGGGGC
TCCCCCGCGC ACTACACACC CCTCACCCAT CCGGTCTCGG CGCCCTCTTC CTCGGGATCC
CCACTGTACG AAGGGGCGGC CGCGGCCACA GACATCGTGG ACAGCCAGTA CGACGCCGCA
GCCCAAGGCC GCCTCATAGC CTCATGGACA CCTGTGTCGC CACCTTCCAT GTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu17741D
Sequence Information ORF Nucleotide Sequence (Length: 1308bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Homo sapiens (human)
Product brachyury protein isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE785653.1, BU190437.1, AJ001699.1, AL627443.6 and BC098425.1. This sequence is a reference standard in the RefSeqGene project. On Jul 11, 2012 this sequence version replaced gi:19743811. Summary: The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AJ001699.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG
TACTCCCAAT GGGGGTGGCT TCTTCCTGGA ACCAGCACCC TGTGTCCACC TGCAAATCCT
CATCCTCAGT TTGGAGGTGC CCTCTCCCTC CCCTCCACGC ACAGCTGTGA CAGGTACCCA
ACCCTGAGGA GCCACCGGTC CTCACCCTAC CCCAGCCCCT ATGCTCATCG GAACAATTCT
CCAACCTATT CTGACAACTC ACCTGCATGT TTATCCATGC TGCAATCCCA TGACAATTGG
TCCAGCCTTG GAATGCCTGC CCATCCCAGC ATGCTCCCCG TGAGCCACAA TGCCAGCCCA
CCTACCAGCT CCAGTCAGTA CCCCAGCCTG TGGTCTGTGA GCAACGGCGC CGTCACCCCG
GGCTCCCAGG CAGCAGCCGT GTCCAACGGG CTGGGGGCCC AGTTCTTCCG GGGCTCCCCC
GCGCACTACA CACCCCTCAC CCATCCGGTC TCGGCGCCCT CTTCCTCGGG ATCCCCACTG
TACGAAGGGG CGGCCGCGGC CACAGACATC GTGGACAGCC AGTACGACGC CGCAGCCCAA
GGCCGCCTCA TAGCCTCATG GACACCTGTG TCGCCACCTT CCATGTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_003172.1
CDS520..1827
Misc Feature(1)220..222(+)
Misc Feature(2)643..1188(+)
Misc Feature(3)706..1176(+)
Misc Feature(4)778..1044(+)
Exon (1)1..342
Gene:T
Gene Synonym:
Exon (2)343..725
Gene:T
Gene Synonym:
Exon (3)726..990
Gene:T
Gene Synonym:
Exon (4)991..1125
Gene:T
Gene Synonym:
Exon (5)1126..1187
Gene:T
Gene Synonym:
Exon (6)1188..1249
Gene:T
Gene Synonym:
Exon (7)1250..1423
Gene:T
Gene Synonym:
Exon (8)1424..1553
Gene:T
Gene Synonym:
Exon (9)1554..2486
Gene:T
Gene Synonym:
Translation
Position Chain Variation Link
7 7 c, t dbSNP:781682069
10 10 g, t dbSNP:576064123
28 28 g, t dbSNP:3099263
34 34 g, t dbSNP:3099264
57 57 a, t dbSNP:757581882
58 58 c, t dbSNP:751929893
60 60 c, g dbSNP:3127339
79 79 a, t dbSNP:368898782
107 107 c, g dbSNP:567948887
108 108 c, t dbSNP:556129052
115 115 a, g dbSNP:35237971
151 151 a, g dbSNP:3757104
186 186 g, t dbSNP:551499002
219 219 g, t dbSNP:371348886
287 287 c, t dbSNP:533425272
314 314 g, t dbSNP:192125239
342 342 a, g dbSNP:550360124
385 385 g, t dbSNP:546408427
408 408 c, t dbSNP:112553716
419 419 c, g dbSNP:528339157
452 452 a, g dbSNP:3099266
479 479 c, g dbSNP:755025433
490 490 c, g dbSNP:754396517
496 496 c, t dbSNP:766904827
499 499 a, g dbSNP:756439333
501 501 a, c dbSNP:750740007
504 504 a, g dbSNP:3734509
507 507 a, g dbSNP:761519813
510 510 a, g dbSNP:187193467
513 513 a, c dbSNP:763746928
516 516 -, g dbSNP:763672068
537 537 c, t dbSNP:762539762
538 538 c, g dbSNP:775360515
541 541 a, t dbSNP:180733987
548 548 a, c, g dbSNP:745665800
552 552 c, g dbSNP:776440268
564 564 c, t dbSNP:770680343
565 565 a, c dbSNP:748415738
568 568 a, g dbSNP:779090198
585 585 a, c dbSNP:534228470
587 587 c, t dbSNP:778891218
589 589 a, c, g dbSNP:563349798
603 603 g, t dbSNP:780438912
609 609 g, t dbSNP:756594225
610 610 a, g dbSNP:750783022
613 613 a, g dbSNP:150278117
633 633 a, g dbSNP:757676401
634 634 a, g dbSNP:760479099
635 635 a, t dbSNP:751315759
637 637 c, g dbSNP:763835047
640 640 g, t dbSNP:762562087
643 643 a, c, g dbSNP:141082493
646 646 a, c dbSNP:370204149
658 658 a, g dbSNP:776406514
660 660 a, g dbSNP:770766611
664 664 a, t dbSNP:760455239
666 666 a, c dbSNP:774559741
680 680 g, t dbSNP:377517710
688 688 a, g dbSNP:768807770
693 693 a, c dbSNP:749347520
709 709 g, t dbSNP:780139385
717 717 a, g dbSNP:769733462
746 746 a, g dbSNP:754517228
748 748 g, t dbSNP:753229275
768 768 c, g dbSNP:370830801
779 779 c, t dbSNP:376160217
796 796 c, g dbSNP:373492906
804 804 g, t dbSNP:756004401
807 807 a, g dbSNP:750329241
813 813 c, t dbSNP:920961
819 819 c, g dbSNP:761502724
821 821 g, t dbSNP:775848412
831 831 a, c, t dbSNP:759679493
835 835 a, g dbSNP:776807169
838 838 c, g dbSNP:771599771
846 846 g, t dbSNP:747562377
850 850 c, t dbSNP:773839438
851 851 c, t dbSNP:772551453
852 852 a, c, g dbSNP:778637353
855 855 c, g dbSNP:200980847
857 857 -, g dbSNP:757779131
863 863 c, t dbSNP:754536703
877 877 c, t dbSNP:201323620
881 881 g, t dbSNP:374750210
882 882 c, t dbSNP:1056048
885 885 c, t dbSNP:201970663
900 900 c, t dbSNP:767353156
903 903 a, c, t dbSNP:751255909
918 918 a, g dbSNP:373205880
919 919 a, g dbSNP:759641403
921 921 c, t dbSNP:776964790
934 934 g, t dbSNP:766618894
938 938 c, t dbSNP:368969464
939 939 c, t dbSNP:376242587
940 940 a, g dbSNP:371635418
979 979 c, g dbSNP:772397551
984 984 c, g dbSNP:748566677
987 987 a, c, t dbSNP:368328281
993 993 c, g dbSNP:368405992
1002 1002 c, t dbSNP:763305561
1005 1005 c, t dbSNP:775129967
1018 1018 a, g dbSNP:546389815
1020 1020 a, g dbSNP:376732326
1031 1031 a, g dbSNP:587777303
1042 1042 a, g dbSNP:780531257
1049 1049 a, g dbSNP:2305089
1055 1055 a, g dbSNP:746971152
1058 1058 a, c, g dbSNP:758269436
1063 1063 a, c dbSNP:752478625
1064 1064 c, t dbSNP:780442577
1066 1066 a, c dbSNP:201970019
1072 1072 a, c dbSNP:750627725
1089 1089 c, t dbSNP:541803907
1090 1090 c, g dbSNP:761937886
1091 1091 a, g dbSNP:752074396
1099 1099 g, t dbSNP:764519598
1101 1101 a, g dbSNP:763321958
1119 1119 c, t dbSNP:374566002
1148 1148 a, t dbSNP:748143125
1189 1189 a, c dbSNP:758453282
1192 1192 g, t dbSNP:571236975
1195 1195 c, t dbSNP:774760380
1199 1199 a, t dbSNP:755308093
1217 1217 c, g dbSNP:754178278
1218 1218 c, t dbSNP:141018360
1219 1219 a, g dbSNP:537568755
1222 1222 a, g dbSNP:374862419
1234 1234 c, t dbSNP:370348152
1245 1245 c, g dbSNP:761403739
1249 1249 c, t dbSNP:774434198
1250 1250 a, g dbSNP:769691133
1251 1251 c, g dbSNP:759325329
1253 1253 c, g dbSNP:369452447
1279 1279 c, g dbSNP:3127336
1281 1281 a, g dbSNP:772229729
1283 1283 g, t dbSNP:748254170
1287 1287 a, t dbSNP:539561632
1288 1288 c, t dbSNP:768592312
1289 1289 c, g dbSNP:749862609
1297 1297 c, t dbSNP:200268852
1298 1298 c, t dbSNP:756506255
1302 1302 c, t dbSNP:550364175
1308 1308 a, g dbSNP:781015050
1316 1316 a, g, t dbSNP:373379157
1321 1321 c, t dbSNP:751227884
1337 1337 c, t dbSNP:35694129
1338 1338 a, g dbSNP:369239526
1353 1353 g, t dbSNP:752749538
1355 1355 a, g dbSNP:139829897
1357 1357 c, t dbSNP:759343495
1360 1360 a, g dbSNP:776366565
1369 1369 a, c dbSNP:770678347
1370 1370 a, g dbSNP:762068341
1375 1375 a, c, t dbSNP:768845167
1376 1376 a, g dbSNP:527987217
1380 1380 a, c dbSNP:776077161
1400 1400 a, g dbSNP:770375711
1408 1408 c, t dbSNP:746266432
1409 1409 a, g dbSNP:781720077
1414 1414 a, t dbSNP:757674783
1419 1419 a, t dbSNP:746736950
1427 1427 a, g, t dbSNP:748045953
1444 1444 a, g dbSNP:369534625
1447 1447 c, t dbSNP:755916571
1454 1454 a, c dbSNP:143862444
1455 1455 c, t dbSNP:541928116
1456 1456 a, g, t dbSNP:376805428
1464 1464 a, g dbSNP:199515427
1473 1473 c, t dbSNP:775760510
1475 1475 a, g dbSNP:552884807
1479 1479 -, aa dbSNP:781706833
1479 1479 g, t dbSNP:759687323
1480 1480 -, g dbSNP:771364595
1480 1480 g, t dbSNP:777285628
1481 1481 c, t dbSNP:771510108
1482 1482 c, t dbSNP:267600890
1483 1483 a, t dbSNP:761128287
1485 1485 a, c dbSNP:773484640
1494 1494 a, g dbSNP:772457578
1496 1496 c, t dbSNP:747810651
1501 1501 -, c dbSNP:746662856
1508 1508 c, g, t dbSNP:375777542
1511 1511 c, t dbSNP:748736752
1518 1518 c, g, t dbSNP:116620192
1519 1519 a, g dbSNP:768390079
1532 1532 c, t dbSNP:117097130
1533 1533 a, c dbSNP:757063703
1535 1535 a, g dbSNP:779700774
1545 1545 c, t dbSNP:200600273
1547 1547 a, g dbSNP:372221322
1562 1562 c, t dbSNP:766423451
1563 1563 c, t dbSNP:756211870
1584 1584 c, t dbSNP:200879575
1585 1585 a, g dbSNP:3127328
1587 1587 c, t dbSNP:762185412
1589 1589 c, t dbSNP:774657641
1590 1590 c, t dbSNP:764411092
1591 1591 a, g dbSNP:77703807
1596 1596 c, t dbSNP:775111459
1598 1598 c, t dbSNP:769256401
1599 1599 a, g dbSNP:745320047
1609 1609 c, g dbSNP:776449248
1618 1618 a, g dbSNP:35292451
1625 1625 a, g dbSNP:3816300
1626 1626 c, t dbSNP:369470652
1627 1627 a, g, t dbSNP:749584982
1629 1629 a, g dbSNP:780253490
1631 1631 c, t dbSNP:375108148
1635 1635 a, g dbSNP:561656601
1636 1636 g, t dbSNP:750466703
1640 1640 a, c dbSNP:147494273
1648 1648 c, t dbSNP:143094140
1649 1649 a, g dbSNP:368179445
1652 1652 g, t dbSNP:764501069
1659 1659 c, t dbSNP:763303937
1660 1660 a, g dbSNP:775012230
1661 1661 c, t dbSNP:764888773
1662 1662 a, g, t dbSNP:776170985
1663 1663 c, t dbSNP:573464240
1666 1666 c, t dbSNP:770321070
1668 1668 c, t dbSNP:746996355
1673 1673 c, t dbSNP:773275146
1677 1677 c, t dbSNP:543190873
1681 1681 c, t dbSNP:747724104
1682 1682 a, g dbSNP:139057807
1684 1684 a, c dbSNP:780160101
1685 1685 c, t dbSNP:756318370
1686 1686 a, g dbSNP:143219390
1691 1691 a, c dbSNP:149220881
1693 1693 g, t dbSNP:138300044
1694 1694 c, t dbSNP:373982301
1695 1695 a, g dbSNP:35819705
1698 1698 c, g dbSNP:778247718
1699 1699 g, t dbSNP:758818312
1706 1706 c, t dbSNP:182159312
1707 1707 a, g dbSNP:764835063
1717 1717 c, g dbSNP:759260639
1718 1718 c, t dbSNP:376697758
1722 1722 c, t dbSNP:765996548
1723 1723 a, g dbSNP:34517945
1728 1728 a, g dbSNP:773343096
1730 1730 a, c, t dbSNP:761554301
1734 1734 c, t dbSNP:201872052
1736 1736 c, t dbSNP:537883499
1739 1739 c, g dbSNP:746115753
1742 1742 -, ggccac dbSNP:777285210
1749 1749 c, t dbSNP:781204071
1750 1750 a, g dbSNP:771064715
1755 1755 c, t dbSNP:747076395
1757 1757 a, g dbSNP:567735651
1764 1764 c, t dbSNP:375588439
1765 1765 a, g dbSNP:753100833
1767 1767 c, t dbSNP:370987280
1768 1768 a, g dbSNP:755234783
1770 1770 c, t dbSNP:372419736
1771 1771 a, g dbSNP:766120230
1775 1775 c, t dbSNP:760313386
1777 1777 c, t dbSNP:749923447
1783 1783 c, t dbSNP:767098205
1784 1784 a, g dbSNP:761833646
1790 1790 c, t dbSNP:146841649
1796 1796 a, c dbSNP:768501878
1800 1800 a, g dbSNP:144704487
1804 1804 c, t dbSNP:776894476
1808 1808 g, t dbSNP:771117935
1811 1811 c, t dbSNP:372990504
1812 1812 a, g dbSNP:140876874
1815 1815 a, t dbSNP:772086874
1816 1816 c, t dbSNP:181394470
1817 1817 c, g dbSNP:748697550
1822 1822 a, g dbSNP:779287427
1833 1833 c, g dbSNP:755431891
1845 1845 a, c dbSNP:368784999
1847 1847 c, t dbSNP:377596495
1848 1848 a, g dbSNP:373627873
1857 1857 -, a dbSNP:757977861
1859 1859 c, t dbSNP:199931571
1868 1868 c, g, t dbSNP:756655657
1870 1870 c, t dbSNP:751580961
1871 1871 a, g dbSNP:200998218
1873 1873 a, g dbSNP:762853565
1874 1874 c, g dbSNP:775232029
1875 1875 c, g dbSNP:764968975
1905 1905 a, g dbSNP:550527863
1951 1951 a, t dbSNP:528914986
1956 1956 c, t dbSNP:561738872
1965 1965 a, g dbSNP:539921601
1981 1981 c, t dbSNP:79882341
2011 2011 a, t dbSNP:560625615
2020 2020 a, c dbSNP:778091431
2030 2030 c, t dbSNP:1056050
2051 2051 a, c dbSNP:1056051
2056 2056 g, t dbSNP:556932718
2061 2061 c, t dbSNP:565758718
2077 2077 c, t dbSNP:3816302
2100 2100 c, t dbSNP:35606910
2105 2105 a, g dbSNP:3099270
2157 2157 c, t dbSNP:533997416
2166 2166 a, g dbSNP:3816304
2168 2168 a, c dbSNP:756117325
2187 2187 a, g dbSNP:1056053
2202 2202 a, c dbSNP:767203205
2212 2212 a, t dbSNP:761167980
2271 2271 a, g dbSNP:539277276
2298 2298 c, t dbSNP:773897685
2344 2344 c, t dbSNP:112492682
2345 2345 c, g dbSNP:568794612
2350 2350 g, t dbSNP:3127327
2369 2369 a, c dbSNP:550567543
2372 2372 c, t dbSNP:768196150
2384 2384 c, t dbSNP:529208108
2447 2447 g, t dbSNP:746353519
2466 2466 a, c dbSNP:1134481
2473 2473 c, t dbSNP:1134482
2484 2484 a, g dbSNP:747408295

Target ORF information:

RefSeq Version NM_003181
Organism Homo sapiens (human)
Definition Homo sapiens T brachyury transcription factor (T), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_003181

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG
TACTCCCAAT GGGGGTGGCT TCTTCCTGGA ACCAGCACCC TGTGTCCACC TGCAAATCCT
CATCCTCAGT TTGGAGGTGC CCTCTCCCTC CCCTCCACGC ACAGCTGTGA CAGGTACCCA
ACCCTGAGGA GCCACCGGTC CTCACCCTAC CCCAGCCCCT ATGCTCATCG GAACAATTCT
CCAACCTATT CTGACAACTC ACCTGCATGT TTATCCATGC TGCAATCCCA TGACAATTGG
TCCAGCCTTG GAATGCCTGC CCATCCCAGC ATGCTCCCCG TGAGCCACAA TGCCAGCCCA
CCTACCAGCT CCAGTCAGTA CCCCAGCCTG TGGTCTGTGA GCAACGGCGC CGTCACCCCG
GGCTCCCAGG CAGCAGCCGT GTCCAACGGG CTGGGGGCCC AGTTCTTCCG GGGCTCCCCC
GCGCACTACA CACCCCTCAC CCATCCGGTC TCGGCGCCCT CTTCCTCGGG ATCCCCACTG
TACGAAGGGG CGGCCGCGGC CACAGACATC GTGGACAGCC AGTACGACGC CGCAGCCCAA
GGCCGCCTCA TAGCCTCATG GACACCTGTG TCGCCACCTT CCATGTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu17747D
Sequence Information ORF Nucleotide Sequence (Length: 1134bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Homo sapiens (human)
Product brachyury protein isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE785653.1, BC098425.1 and AL627443.6. Summary: The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC098425.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG
TACTCCCAAT CCTATTCTGA CAACTCACCT GCATGTTTAT CCATGCTGCA ATCCCATGAC
AATTGGTCCA GCCTTGGAAT GCCTGCCCAT CCCAGCATGC TCCCCGTGAG CCACAATGCC
AGCCCACCTA CCAGCTCCAG TCAGTACCCC AGCCTGTGGT CTGTGAGCAA CGGCGCCGTC
ACCCCGGGCT CCCAGGCAGC AGCCGTGTCC AACGGGCTGG GGGCCCAGTT CTTCCGGGGC
TCCCCCGCGC ACTACACACC CCTCACCCAT CCGGTCTCGG CGCCCTCTTC CTCGGGATCC
CCACTGTACG AAGGGGCGGC CGCGGCCACA GACATCGTGG ACAGCCAGTA CGACGCCGCA
GCCCAAGGCC GCCTCATAGC CTCATGGACA CCTGTGTCGC CACCTTCCAT GTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001257413.1
CDS427..1560
Misc Feature(1)220..222(+)
Misc Feature(2)550..1095(+)
Misc Feature(3)613..1083(+)
Misc Feature(4)685..951(+)
Misc Feature(5)937..1263(+)
Exon (1)1..342
Gene:T
Gene Synonym:
Exon (2)343..632
Gene:T
Gene Synonym:
Exon (3)633..897
Gene:T
Gene Synonym:
Exon (4)898..1032
Gene:T
Gene Synonym:
Exon (5)1033..1094
Gene:T
Gene Synonym:
Exon (6)1095..1156
Gene:T
Gene Synonym:
Exon (7)1157..1286
Gene:T
Gene Synonym:
Exon (8)1287..2219
Gene:T
Gene Synonym:
Translation
Position Chain Variation Link
7 7 c, t dbSNP:781682069
10 10 g, t dbSNP:576064123
28 28 g, t dbSNP:3099263
34 34 g, t dbSNP:3099264
57 57 a, t dbSNP:757581882
58 58 c, t dbSNP:751929893
60 60 c, g dbSNP:3127339
79 79 a, t dbSNP:368898782
107 107 c, g dbSNP:567948887
108 108 c, t dbSNP:556129052
115 115 a, g dbSNP:35237971
151 151 a, g dbSNP:3757104
186 186 g, t dbSNP:551499002
219 219 g, t dbSNP:371348886
287 287 c, t dbSNP:533425272
314 314 g, t dbSNP:192125239
342 342 a, g dbSNP:550360124
359 359 a, g dbSNP:3099266
386 386 c, g dbSNP:755025433
397 397 c, g dbSNP:754396517
403 403 c, t dbSNP:766904827
406 406 a, g dbSNP:756439333
408 408 a, c dbSNP:750740007
411 411 a, g dbSNP:3734509
414 414 a, g dbSNP:761519813
417 417 a, g dbSNP:187193467
420 420 a, c dbSNP:763746928
423 423 -, g dbSNP:763672068
444 444 c, t dbSNP:762539762
445 445 c, g dbSNP:775360515
448 448 a, t dbSNP:180733987
455 455 a, c, g dbSNP:745665800
459 459 c, g dbSNP:776440268
471 471 c, t dbSNP:770680343
472 472 a, c dbSNP:748415738
475 475 a, g dbSNP:779090198
492 492 a, c dbSNP:534228470
494 494 c, t dbSNP:778891218
496 496 a, c, g dbSNP:563349798
510 510 g, t dbSNP:780438912
516 516 g, t dbSNP:756594225
517 517 a, g dbSNP:750783022
520 520 a, g dbSNP:150278117
540 540 a, g dbSNP:757676401
541 541 a, g dbSNP:760479099
542 542 a, t dbSNP:751315759
544 544 c, g dbSNP:763835047
547 547 g, t dbSNP:762562087
550 550 a, c, g dbSNP:141082493
553 553 a, c dbSNP:370204149
565 565 a, g dbSNP:776406514
567 567 a, g dbSNP:770766611
571 571 a, t dbSNP:760455239
573 573 a, c dbSNP:774559741
587 587 g, t dbSNP:377517710
595 595 a, g dbSNP:768807770
600 600 a, c dbSNP:749347520
616 616 g, t dbSNP:780139385
624 624 a, g dbSNP:769733462
653 653 a, g dbSNP:754517228
655 655 g, t dbSNP:753229275
675 675 c, g dbSNP:370830801
686 686 c, t dbSNP:376160217
703 703 c, g dbSNP:373492906
711 711 g, t dbSNP:756004401
714 714 a, g dbSNP:750329241
720 720 c, t dbSNP:920961
726 726 c, g dbSNP:761502724
728 728 g, t dbSNP:775848412
738 738 a, c, t dbSNP:759679493
742 742 a, g dbSNP:776807169
745 745 c, g dbSNP:771599771
753 753 g, t dbSNP:747562377
757 757 c, t dbSNP:773839438
758 758 c, t dbSNP:772551453
759 759 a, c, g dbSNP:778637353
762 762 c, g dbSNP:200980847
764 764 -, g dbSNP:757779131
770 770 c, t dbSNP:754536703
784 784 c, t dbSNP:201323620
788 788 g, t dbSNP:374750210
789 789 c, t dbSNP:1056048
792 792 c, t dbSNP:201970663
807 807 c, t dbSNP:767353156
810 810 a, c, t dbSNP:751255909
825 825 a, g dbSNP:373205880
826 826 a, g dbSNP:759641403
828 828 c, t dbSNP:776964790
841 841 g, t dbSNP:766618894
845 845 c, t dbSNP:368969464
846 846 c, t dbSNP:376242587
847 847 a, g dbSNP:371635418
886 886 c, g dbSNP:772397551
891 891 c, g dbSNP:748566677
894 894 a, c, t dbSNP:368328281
900 900 c, g dbSNP:368405992
909 909 c, t dbSNP:763305561
912 912 c, t dbSNP:775129967
925 925 a, g dbSNP:546389815
927 927 a, g dbSNP:376732326
938 938 a, g dbSNP:587777303
949 949 a, g dbSNP:780531257
956 956 a, g dbSNP:2305089
962 962 a, g dbSNP:746971152
965 965 a, c, g dbSNP:758269436
970 970 a, c dbSNP:752478625
971 971 c, t dbSNP:780442577
973 973 a, c dbSNP:201970019
979 979 a, c dbSNP:750627725
996 996 c, t dbSNP:541803907
997 997 c, g dbSNP:761937886
998 998 a, g dbSNP:752074396
1006 1006 g, t dbSNP:764519598
1008 1008 a, g dbSNP:763321958
1026 1026 c, t dbSNP:374566002
1055 1055 a, t dbSNP:748143125
1096 1096 a, c dbSNP:758453282
1099 1099 g, t dbSNP:571236975
1102 1102 c, t dbSNP:774760380
1106 1106 a, t dbSNP:755308093
1124 1124 c, g dbSNP:754178278
1125 1125 c, t dbSNP:141018360
1126 1126 a, g dbSNP:537568755
1129 1129 a, g dbSNP:374862419
1141 1141 c, t dbSNP:370348152
1152 1152 c, g dbSNP:761403739
1156 1156 c, t dbSNP:774434198
1160 1160 a, g, t dbSNP:748045953
1177 1177 a, g dbSNP:369534625
1180 1180 c, t dbSNP:755916571
1187 1187 a, c dbSNP:143862444
1188 1188 c, t dbSNP:541928116
1189 1189 a, g, t dbSNP:376805428
1197 1197 a, g dbSNP:199515427
1206 1206 c, t dbSNP:775760510
1208 1208 a, g dbSNP:552884807
1212 1212 -, aa dbSNP:781706833
1212 1212 g, t dbSNP:759687323
1213 1213 -, g dbSNP:771364595
1213 1213 g, t dbSNP:777285628
1214 1214 c, t dbSNP:771510108
1215 1215 c, t dbSNP:267600890
1216 1216 a, t dbSNP:761128287
1218 1218 a, c dbSNP:773484640
1227 1227 a, g dbSNP:772457578
1229 1229 c, t dbSNP:747810651
1234 1234 -, c dbSNP:746662856
1241 1241 c, g, t dbSNP:375777542
1244 1244 c, t dbSNP:748736752
1251 1251 c, g, t dbSNP:116620192
1252 1252 a, g dbSNP:768390079
1265 1265 c, t dbSNP:117097130
1266 1266 a, c dbSNP:757063703
1268 1268 a, g dbSNP:779700774
1278 1278 c, t dbSNP:200600273
1280 1280 a, g dbSNP:372221322
1295 1295 c, t dbSNP:766423451
1296 1296 c, t dbSNP:756211870
1317 1317 c, t dbSNP:200879575
1318 1318 a, g dbSNP:3127328
1320 1320 c, t dbSNP:762185412
1322 1322 c, t dbSNP:774657641
1323 1323 c, t dbSNP:764411092
1324 1324 a, g dbSNP:77703807
1329 1329 c, t dbSNP:775111459
1331 1331 c, t dbSNP:769256401
1332 1332 a, g dbSNP:745320047
1342 1342 c, g dbSNP:776449248
1351 1351 a, g dbSNP:35292451
1358 1358 a, g dbSNP:3816300
1359 1359 c, t dbSNP:369470652
1360 1360 a, g, t dbSNP:749584982
1362 1362 a, g dbSNP:780253490
1364 1364 c, t dbSNP:375108148
1368 1368 a, g dbSNP:561656601
1369 1369 g, t dbSNP:750466703
1373 1373 a, c dbSNP:147494273
1381 1381 c, t dbSNP:143094140
1382 1382 a, g dbSNP:368179445
1385 1385 g, t dbSNP:764501069
1392 1392 c, t dbSNP:763303937
1393 1393 a, g dbSNP:775012230
1394 1394 c, t dbSNP:764888773
1395 1395 a, g, t dbSNP:776170985
1396 1396 c, t dbSNP:573464240
1399 1399 c, t dbSNP:770321070
1401 1401 c, t dbSNP:746996355
1406 1406 c, t dbSNP:773275146
1410 1410 c, t dbSNP:543190873
1414 1414 c, t dbSNP:747724104
1415 1415 a, g dbSNP:139057807
1417 1417 a, c dbSNP:780160101
1418 1418 c, t dbSNP:756318370
1419 1419 a, g dbSNP:143219390
1424 1424 a, c dbSNP:149220881
1426 1426 g, t dbSNP:138300044
1427 1427 c, t dbSNP:373982301
1428 1428 a, g dbSNP:35819705
1431 1431 c, g dbSNP:778247718
1432 1432 g, t dbSNP:758818312
1439 1439 c, t dbSNP:182159312
1440 1440 a, g dbSNP:764835063
1450 1450 c, g dbSNP:759260639
1451 1451 c, t dbSNP:376697758
1455 1455 c, t dbSNP:765996548
1456 1456 a, g dbSNP:34517945
1461 1461 a, g dbSNP:773343096
1463 1463 a, c, t dbSNP:761554301
1467 1467 c, t dbSNP:201872052
1469 1469 c, t dbSNP:537883499
1472 1472 c, g dbSNP:746115753
1475 1475 -, ggccac dbSNP:777285210
1482 1482 c, t dbSNP:781204071
1483 1483 a, g dbSNP:771064715
1488 1488 c, t dbSNP:747076395
1490 1490 a, g dbSNP:567735651
1497 1497 c, t dbSNP:375588439
1498 1498 a, g dbSNP:753100833
1500 1500 c, t dbSNP:370987280
1501 1501 a, g dbSNP:755234783
1503 1503 c, t dbSNP:372419736
1504 1504 a, g dbSNP:766120230
1508 1508 c, t dbSNP:760313386
1510 1510 c, t dbSNP:749923447
1516 1516 c, t dbSNP:767098205
1517 1517 a, g dbSNP:761833646
1523 1523 c, t dbSNP:146841649
1529 1529 a, c dbSNP:768501878
1533 1533 a, g dbSNP:144704487
1537 1537 c, t dbSNP:776894476
1541 1541 g, t dbSNP:771117935
1544 1544 c, t dbSNP:372990504
1545 1545 a, g dbSNP:140876874
1548 1548 a, t dbSNP:772086874
1549 1549 c, t dbSNP:181394470
1550 1550 c, g dbSNP:748697550
1555 1555 a, g dbSNP:779287427
1566 1566 c, g dbSNP:755431891
1578 1578 a, c dbSNP:368784999
1580 1580 c, t dbSNP:377596495
1581 1581 a, g dbSNP:373627873
1590 1590 -, a dbSNP:757977861
1592 1592 c, t dbSNP:199931571
1601 1601 c, g, t dbSNP:756655657
1603 1603 c, t dbSNP:751580961
1604 1604 a, g dbSNP:200998218
1606 1606 a, g dbSNP:762853565
1607 1607 c, g dbSNP:775232029
1608 1608 c, g dbSNP:764968975
1638 1638 a, g dbSNP:550527863
1684 1684 a, t dbSNP:528914986
1689 1689 c, t dbSNP:561738872
1698 1698 a, g dbSNP:539921601
1714 1714 c, t dbSNP:79882341
1744 1744 a, t dbSNP:560625615
1753 1753 a, c dbSNP:778091431
1763 1763 c, t dbSNP:1056050
1784 1784 a, c dbSNP:1056051
1789 1789 g, t dbSNP:556932718
1794 1794 c, t dbSNP:565758718
1810 1810 c, t dbSNP:3816302
1833 1833 c, t dbSNP:35606910
1838 1838 a, g dbSNP:3099270
1890 1890 c, t dbSNP:533997416
1899 1899 a, g dbSNP:3816304
1901 1901 a, c dbSNP:756117325
1920 1920 a, g dbSNP:1056053
1935 1935 a, c dbSNP:767203205
1945 1945 a, t dbSNP:761167980
2004 2004 a, g dbSNP:539277276
2031 2031 c, t dbSNP:773897685
2077 2077 c, t dbSNP:112492682
2078 2078 c, g dbSNP:568794612
2083 2083 g, t dbSNP:3127327
2102 2102 a, c dbSNP:550567543
2105 2105 c, t dbSNP:768196150
2117 2117 c, t dbSNP:529208108
2180 2180 g, t dbSNP:746353519
2199 2199 a, c dbSNP:1134481
2206 2206 c, t dbSNP:1134482
2217 2217 a, g dbSNP:747408295

Target ORF information:

RefSeq Version NM_001270484
Organism Homo sapiens (human)
Definition Homo sapiens T brachyury transcription factor (T), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001270484

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG
TACTCCCAAT CCTATTCTGA CAACTCACCT GCATGTTTAT CCATGCTGCA ATCCCATGAC
AATTGGTCCA GCCTTGGAAT GCCTGCCCAT CCCAGCATGC TCCCCGTGAG CCACAATGCC
AGCCCACCTA CCAGCTCCAG TCAGTACCCC AGCCTGTGGT CTGTGAGCAA CGGCGCCGTC
ACCCCGGGCT CCCAGGCAGC AGCCGTGTCC AACGGGCTGG GGGCCCAGTT CTTCCGGGGC
TCCCCCGCGC ACTACACACC CCTCACCCAT CCGGTCTCGG CGCCCTCTTC CTCGGGATCC
CCACTGTACG AAGGGGCGGC CGCGGCCACA GACATCGTGG ACAGCCAGTA CGACGCCGCA
GCCCAAGGCC GCCTCATAGC CTCATGGACA CCTGTGTCGC CACCTTCCAT GTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.