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T cDNA ORF clone, Homo sapiens (human)

Gene Symbol T
Entrez Gene ID 6862
Full Name T brachyury transcription factor
Synonyms SAVA, TFT
General protein information
Preferred Names
brachyury protein
Names
brachyury protein
protein T
T brachyury homolog
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6q27

Summary The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]. lac of sum
Disorder MIM:

601397

Disorder Html: {Neural tube defects, susceptibility to}, 182940 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011536080 XP_011534382 brachyury protein isoform X2
XM_011536081 XP_011534383 brachyury protein isoform X1
NM_003181 NP_003172 brachyury protein isoform 1
NM_001270484 NP_001257413 brachyury protein isoform 2



Homo sapiens (human) T NP_003172.1
Pan troglodytes (chimpanzee) T XP_527563.3
Macaca mulatta (Rhesus monkey) T XP_001101514.1
Canis lupus familiaris (dog) T NP_001003092.1
Bos taurus (cattle) T NP_001179914.1
Mus musculus (house mouse) T NP_033335.1
Rattus norvegicus (Norway rat) T NP_001099679.1
Gallus gallus (chicken) T NP_990271.1
Danio rerio (zebrafish) LOC100004296 XP_001343633.3
Xenopus (Silurana) tropicalis (western clawed frog) t NP_001008139.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following T gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the T cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu60837 XM_011536080 PREDICTED: Homo sapiens T, brachyury homolog (mouse) (T), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu17747 XM_011536081 PREDICTED: Homo sapiens T, brachyury homolog (mouse) (T), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu17741
NM_003181 Homo sapiens T brachyury transcription factor (T), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$179.50-$251.30
$359.00
OHu17747 NM_001270484 Homo sapiens T brachyury transcription factor (T), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu60837
Clone ID Related Accession (Same CDS sequence) XM_011536080
Accession Version XM_011536080.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1311bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product brachyury protein isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_025741.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG
TACTCCCAAT CAGGGGGGTG GCTTCTTCCT GGAACCAGCA CCCTGTGTCC ACCTGCAAAT
CCTCATCCTC AGTTTGGAGG TGCCCTCTCC CTCCCCTCCA CGCACAGCTG TGACAGGTAC
CCAACCCTGA GGAGCCACCG GTCCTCACCC TACCCCAGCC CCTATGCTCA TCGGAACAAT
TCTCCAACCT ATTCTGACAA CTCACCTGCA TGTTTATCCA TGCTGCAATC CCATGACAAT
TGGTCCAGCC TTGGAATGCC TGCCCATCCC AGCATGCTCC CCGTGAGCCA CAATGCCAGC
CCACCTACCA GCTCCAGTCA GTACCCCAGC CTGTGGTCTG TGAGCAACGG CGCCGTCACC
CCGGGCTCCC AGGCAGCAGC CGTGTCCAAC GGGCTGGGGG CCCAGTTCTT CCGGGGCTCC
CCCGCGCACT ACACACCCCT CACCCATCCG GTCTCGGCGC CCTCTTCCTC GGGATCCCCA
CTGTACGAAG GGGCGGCCGC GGCCACAGAC ATCGTGGACA GCCAGTACGA CGCCGCAGCC
CAAGGCCGCC TCATAGCCTC ATGGACACCT GTGTCGCCAC CTTCCATGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011534382.1
CDS287..1597
Misc Feature(1)410..955(+)
Misc Feature(2)473..943(+)
Misc Feature(3)545..811(+)
Translation

Target ORF information:

RefSeq Version XM_011536080
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens T, brachyury homolog (mouse) (T), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011536080

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG
TACTCCCAAT CAGGGGGGTG GCTTCTTCCT GGAACCAGCA CCCTGTGTCC ACCTGCAAAT
CCTCATCCTC AGTTTGGAGG TGCCCTCTCC CTCCCCTCCA CGCACAGCTG TGACAGGTAC
CCAACCCTGA GGAGCCACCG GTCCTCACCC TACCCCAGCC CCTATGCTCA TCGGAACAAT
TCTCCAACCT ATTCTGACAA CTCACCTGCA TGTTTATCCA TGCTGCAATC CCATGACAAT
TGGTCCAGCC TTGGAATGCC TGCCCATCCC AGCATGCTCC CCGTGAGCCA CAATGCCAGC
CCACCTACCA GCTCCAGTCA GTACCCCAGC CTGTGGTCTG TGAGCAACGG CGCCGTCACC
CCGGGCTCCC AGGCAGCAGC CGTGTCCAAC GGGCTGGGGG CCCAGTTCTT CCGGGGCTCC
CCCGCGCACT ACACACCCCT CACCCATCCG GTCTCGGCGC CCTCTTCCTC GGGATCCCCA
CTGTACGAAG GGGCGGCCGC GGCCACAGAC ATCGTGGACA GCCAGTACGA CGCCGCAGCC
CAAGGCCGCC TCATAGCCTC ATGGACACCT GTGTCGCCAC CTTCCATGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17747
Clone ID Related Accession (Same CDS sequence) NM_001270484 , XM_011536081
Accession Version XM_011536081.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1134bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product brachyury protein isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_025741.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG
TACTCCCAAT CCTATTCTGA CAACTCACCT GCATGTTTAT CCATGCTGCA ATCCCATGAC
AATTGGTCCA GCCTTGGAAT GCCTGCCCAT CCCAGCATGC TCCCCGTGAG CCACAATGCC
AGCCCACCTA CCAGCTCCAG TCAGTACCCC AGCCTGTGGT CTGTGAGCAA CGGCGCCGTC
ACCCCGGGCT CCCAGGCAGC AGCCGTGTCC AACGGGCTGG GGGCCCAGTT CTTCCGGGGC
TCCCCCGCGC ACTACACACC CCTCACCCAT CCGGTCTCGG CGCCCTCTTC CTCGGGATCC
CCACTGTACG AAGGGGCGGC CGCGGCCACA GACATCGTGG ACAGCCAGTA CGACGCCGCA
GCCCAAGGCC GCCTCATAGC CTCATGGACA CCTGTGTCGC CACCTTCCAT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011534383.1
CDS153..1286
Misc Feature(1)276..821(+)
Misc Feature(2)339..809(+)
Misc Feature(3)411..677(+)
Misc Feature(4)663..989(+)
Translation

Target ORF information:

RefSeq Version XM_011536081
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens T, brachyury homolog (mouse) (T), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011536081

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG
TACTCCCAAT CCTATTCTGA CAACTCACCT GCATGTTTAT CCATGCTGCA ATCCCATGAC
AATTGGTCCA GCCTTGGAAT GCCTGCCCAT CCCAGCATGC TCCCCGTGAG CCACAATGCC
AGCCCACCTA CCAGCTCCAG TCAGTACCCC AGCCTGTGGT CTGTGAGCAA CGGCGCCGTC
ACCCCGGGCT CCCAGGCAGC AGCCGTGTCC AACGGGCTGG GGGCCCAGTT CTTCCGGGGC
TCCCCCGCGC ACTACACACC CCTCACCCAT CCGGTCTCGG CGCCCTCTTC CTCGGGATCC
CCACTGTACG AAGGGGCGGC CGCGGCCACA GACATCGTGG ACAGCCAGTA CGACGCCGCA
GCCCAAGGCC GCCTCATAGC CTCATGGACA CCTGTGTCGC CACCTTCCAT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17741
Clone ID Related Accession (Same CDS sequence) NM_003181
Accession Version NM_003181.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1308bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Homo sapiens (human)
Product brachyury protein isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE785653.1, BU190437.1, AJ001699.1, AL627443.6 and BC098425.1. This sequence is a reference standard in the RefSeqGene project. On Jul 11, 2012 this sequence version replaced gi:19743811. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AJ001699.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG
TACTCCCAAT GGGGGTGGCT TCTTCCTGGA ACCAGCACCC TGTGTCCACC TGCAAATCCT
CATCCTCAGT TTGGAGGTGC CCTCTCCCTC CCCTCCACGC ACAGCTGTGA CAGGTACCCA
ACCCTGAGGA GCCACCGGTC CTCACCCTAC CCCAGCCCCT ATGCTCATCG GAACAATTCT
CCAACCTATT CTGACAACTC ACCTGCATGT TTATCCATGC TGCAATCCCA TGACAATTGG
TCCAGCCTTG GAATGCCTGC CCATCCCAGC ATGCTCCCCG TGAGCCACAA TGCCAGCCCA
CCTACCAGCT CCAGTCAGTA CCCCAGCCTG TGGTCTGTGA GCAACGGCGC CGTCACCCCG
GGCTCCCAGG CAGCAGCCGT GTCCAACGGG CTGGGGGCCC AGTTCTTCCG GGGCTCCCCC
GCGCACTACA CACCCCTCAC CCATCCGGTC TCGGCGCCCT CTTCCTCGGG ATCCCCACTG
TACGAAGGGG CGGCCGCGGC CACAGACATC GTGGACAGCC AGTACGACGC CGCAGCCCAA
GGCCGCCTCA TAGCCTCATG GACACCTGTG TCGCCACCTT CCATGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003172.1
CDS520..1827
Misc Feature(1)220..222(+)
Misc Feature(2)643..1188(+)
Misc Feature(3)706..1176(+)
Misc Feature(4)778..1044(+)
Exon (1)1..342
Gene:T
Gene Synonym:
Exon (2)343..725
Gene:T
Gene Synonym:
Exon (3)726..990
Gene:T
Gene Synonym:
Exon (4)991..1125
Gene:T
Gene Synonym:
Exon (5)1126..1187
Gene:T
Gene Synonym:
Exon (6)1188..1249
Gene:T
Gene Synonym:
Exon (7)1250..1423
Gene:T
Gene Synonym:
Exon (8)1424..1553
Gene:T
Gene Synonym:
Exon (9)1554..2486
Gene:T
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_003181
Organism Homo sapiens (human)
Definition Homo sapiens T brachyury transcription factor (T), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003181

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG
TACTCCCAAT GGGGGTGGCT TCTTCCTGGA ACCAGCACCC TGTGTCCACC TGCAAATCCT
CATCCTCAGT TTGGAGGTGC CCTCTCCCTC CCCTCCACGC ACAGCTGTGA CAGGTACCCA
ACCCTGAGGA GCCACCGGTC CTCACCCTAC CCCAGCCCCT ATGCTCATCG GAACAATTCT
CCAACCTATT CTGACAACTC ACCTGCATGT TTATCCATGC TGCAATCCCA TGACAATTGG
TCCAGCCTTG GAATGCCTGC CCATCCCAGC ATGCTCCCCG TGAGCCACAA TGCCAGCCCA
CCTACCAGCT CCAGTCAGTA CCCCAGCCTG TGGTCTGTGA GCAACGGCGC CGTCACCCCG
GGCTCCCAGG CAGCAGCCGT GTCCAACGGG CTGGGGGCCC AGTTCTTCCG GGGCTCCCCC
GCGCACTACA CACCCCTCAC CCATCCGGTC TCGGCGCCCT CTTCCTCGGG ATCCCCACTG
TACGAAGGGG CGGCCGCGGC CACAGACATC GTGGACAGCC AGTACGACGC CGCAGCCCAA
GGCCGCCTCA TAGCCTCATG GACACCTGTG TCGCCACCTT CCATGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17747
Clone ID Related Accession (Same CDS sequence) NM_001270484 , XM_011536081
Accession Version NM_001270484.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1134bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Homo sapiens (human)
Product brachyury protein isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE785653.1, BC098425.1 and AL627443.6. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC098425.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG
TACTCCCAAT CCTATTCTGA CAACTCACCT GCATGTTTAT CCATGCTGCA ATCCCATGAC
AATTGGTCCA GCCTTGGAAT GCCTGCCCAT CCCAGCATGC TCCCCGTGAG CCACAATGCC
AGCCCACCTA CCAGCTCCAG TCAGTACCCC AGCCTGTGGT CTGTGAGCAA CGGCGCCGTC
ACCCCGGGCT CCCAGGCAGC AGCCGTGTCC AACGGGCTGG GGGCCCAGTT CTTCCGGGGC
TCCCCCGCGC ACTACACACC CCTCACCCAT CCGGTCTCGG CGCCCTCTTC CTCGGGATCC
CCACTGTACG AAGGGGCGGC CGCGGCCACA GACATCGTGG ACAGCCAGTA CGACGCCGCA
GCCCAAGGCC GCCTCATAGC CTCATGGACA CCTGTGTCGC CACCTTCCAT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001257413.1
CDS427..1560
Misc Feature(1)220..222(+)
Misc Feature(2)550..1095(+)
Misc Feature(3)613..1083(+)
Misc Feature(4)685..951(+)
Misc Feature(5)937..1263(+)
Exon (1)1..342
Gene:T
Gene Synonym:
Exon (2)343..632
Gene:T
Gene Synonym:
Exon (3)633..897
Gene:T
Gene Synonym:
Exon (4)898..1032
Gene:T
Gene Synonym:
Exon (5)1033..1094
Gene:T
Gene Synonym:
Exon (6)1095..1156
Gene:T
Gene Synonym:
Exon (7)1157..1286
Gene:T
Gene Synonym:
Exon (8)1287..2219
Gene:T
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001270484
Organism Homo sapiens (human)
Definition Homo sapiens T brachyury transcription factor (T), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001270484

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG
TACTCCCAAT CCTATTCTGA CAACTCACCT GCATGTTTAT CCATGCTGCA ATCCCATGAC
AATTGGTCCA GCCTTGGAAT GCCTGCCCAT CCCAGCATGC TCCCCGTGAG CCACAATGCC
AGCCCACCTA CCAGCTCCAG TCAGTACCCC AGCCTGTGGT CTGTGAGCAA CGGCGCCGTC
ACCCCGGGCT CCCAGGCAGC AGCCGTGTCC AACGGGCTGG GGGCCCAGTT CTTCCGGGGC
TCCCCCGCGC ACTACACACC CCTCACCCAT CCGGTCTCGG CGCCCTCTTC CTCGGGATCC
CCACTGTACG AAGGGGCGGC CGCGGCCACA GACATCGTGG ACAGCCAGTA CGACGCCGCA
GCCCAAGGCC GCCTCATAGC CTCATGGACA CCTGTGTCGC CACCTTCCAT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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