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ADAM17 ADAM metallopeptidase domain 17 [Homo sapiens (human)]

Gene Symbol ADAM17
Entrez Gene ID 6868
Full Name ADAM metallopeptidase domain 17
Synonyms ADAM18, CD156B, CSVP, NISBD, NISBD1, TACE
General protein information
Preferred Names
disintegrin and metalloproteinase domain-containing protein 17
Names
disintegrin and metalloproteinase domain-containing protein 17
TNF-alpha convertase
snake venom-like protease
TNF-alpha converting enzyme
ADAM metallopeptidase domain 18
tumor necrosis factor, alpha, converting enzyme
NP_003174.3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2p25

Summary This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

603639

Disorder Html:
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu60843 XM_011510375 PREDICTED: Homo sapiens ADAM metallopeptidase domain 17 (ADAM17), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK Not in stock 12-14 Starting from $99.00
OHu60844 XM_011510376 PREDICTED: Homo sapiens ADAM metallopeptidase domain 17 (ADAM17), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu60845 XM_011510377 PREDICTED: Homo sapiens ADAM metallopeptidase domain 17 (ADAM17), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu60845 XM_011510378 PREDICTED: Homo sapiens ADAM metallopeptidase domain 17 (ADAM17), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu21940 NM_003183 Homo sapiens ADAM metallopeptidase domain 17 (ADAM17), mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu60843D
Sequence Information ORF Nucleotide Sequence (Length: 2382bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product disintegrin and metalloproteinase domain-containing protein 17 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005334.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGAAGAAGC TTGATTCTTT GCTCTCAGAC TACGATATTC TCTCTTTATC TAATATCCAG 
CAGCATTCGG TAAGAAAAAG AGATCTACAG ACTTCAACAC ATGTAGAAAC ACTACTAACT
TTTTCAGCTT TGAAAAGGCA TTTTAAATTA TACCTGACAT CAAGTACTGA ACGTTTTTCA
CAAAATTTCA AGGTCGTGGT GGTGGATGGT AAAAACGAAA GCGAGTACAC TGTAAAATGG
CAGGACTTCT TCACTGGACA CGTGGTTGGT GAGCCTGACT CTAGGGTTCT AGCCCACATA
AGAGATGATG ATGTTATAAT CAGAATCAAC ACAGATGGGG CCGAATATAA CATAGAGCCA
CTTTGGAGAT TTGTTAATGA TACCAAAGAC AAAAGAATGT TAGTTTATAA ATCTGAAGAT
ATCAAGAATG TTTCACGTTT GCAGTCTCCA AAAGTGTGTG GTTATTTAAA AGTGGATAAT
GAAGAGTTGC TCCCAAAAGG GTTAGTAGAC AGAGAACCAC CTGAAGAGCT TGTTCATCGA
GTGAAAAGAA GAGCTGACCC AGATCCCATG AAGAACACGT GTAAATTATT GGTGGTAGCA
GATCATCGCT TCTACAGATA CATGGGCAGA GGGGAAGAGA GTACAACTAC AAATTACTTA
ATAGAGCTAA TTGACAGAGT TGATGACATC TATCGGAACA CTTCATGGGA TAATGCAGGT
TTTAAAGGCT ATGGAATACA GATAGAGCAG ATTCGCATTC TCAAGTCTCC ACAAGAGGTA
AAACCTGGTG AAAAGCACTA CAACATGGCA AAAAGTTACC CAAATGAAGA AAAGGATGCT
TGGGATGTGA AGATGTTGCT AGAGCAATTT AGCTTTGATA TAGCTGAGGA AGCATCTAAA
GTTTGCTTGG CACACCTTTT CACATACCAA GATTTTGATA TGGGAACTCT TGGATTAGCT
TATGTTGGCT CTCCCAGAGC AAACAGCCAT GGAGGTGTTT GTCCAAAGGC TTATTATAGC
CCAGTTGGGA AGAAAAATAT CTATTTGAAT AGTGGTTTGA CGAGCACAAA GAATTATGGT
AAAACCATCC TTACAAAGGA AGCTGACCTG GTTACAACTC ATGAATTGGG ACATAATTTT
GGAGCAGAAC ATGATCCGGA TGGTCTAGCA GAATGTGCCC CGAATGAGGA CCAGGGAGGG
AAATATGTCA TGTATCCCAT AGCTGTGAGT GGCGATCACG AGAACAATAA GATGTTTTCA
AACTGCAGTA AACAATCAAT CTATAAGACC ATTGAAAGTA AGGCCCAGGA GTGTTTTCAA
GAACGCAGCA ATAAAGTTTG TGGGAACTCG AGGGTGGATG AAGGAGAAGA GTGTGATCCT
GGCATCATGT ATCTGAACAA CGACACCTGC TGCAACAGCG ACTGCACGTT GAAGGAAGGT
GTCCAGTGCA GTGACAGGAA CAGTCCTTGC TGTAAAAACT GTCAGTTTGA GACTGCCCAG
AAGAAGTGCC AGGAGGCGAT TAATGCTACT TGCAAAGGCG TGTCCTACTG CACAGGTAAT
AGCAGTGAGT GCCCGCCTCC AGGAAATGCT GAAGATGACA CTGTTTGCTT GGATCTTGGC
AAGTGTAAGG ATGGGAAATG CATCCCTTTC TGCGAGAGGG AACAGCAGCT GGAGTCCTGT
GCATGTAATG AAACTGACAA CTCCTGCAAG GTGTGCTGCA GGGACCTTTC TGGCCGCTGT
GTGCCCTATG TCGATGCTGA ACAAAAGAAC TTATTTTTGA GGAAAGGAAA GCCCTGTACA
GTAGGATTTT GTGACATGAA TGGCAAATGT GAGAAACGAG TACAGGATGT AATTGAACGA
TTTTGGGATT TCATTGACCA GCTGAGCATC AATACTTTTG GAAAGTTTTT AGCAGACAAC
ATCGTTGGGT CTGTCCTGGT TTTCTCCTTG ATATTTTGGA TTCCTTTCAG CATTCTTGTC
CATTGTGTGG ATAAGAAATT GGATAAACAG TATGAATCTC TGTCTCTGTT TCACCCCAGT
AACGTCGAAA TGCTGAGCAG CATGGATTCT GCATCGGTTC GCATTATCAA ACCCTTTCCT
GCGCCCCAGA CTCCAGGCCG CCTGCAGCCT GCCCCTGTGA TCCCTTCGGC GCCAGCAGCT
CCAAAACTGG ACCACCAGAG AATGGACACC ATCCAGGAAG ACCCCAGCAC AGACTCACAT
ATGGACGAGG ATGGGTTTGA GAAGGACCCC TTCCCAAATA GCAGCACAGC TGCCAAGTCA
TTTGAGGATC TCACGGACCA TCCGGTCACC AGAAGTGAAA AGGCTGCCTC CTTTAAACTG
CAGCGTCAGA ATCGTGTTGA CAGCAAAGAA ACAGAGTGCTT AA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011508677.1
CDS31..2412
Misc Feature(1)61..438(+)
Misc Feature(2)604..1368(+)
Misc Feature(3)1150..1182(+)
Misc Feature(4)1387..1617(+)
Misc Feature(5)1675..1863(+)
Misc Feature(6)1735..1842(+)
Misc Feature(7)1735..1746(+)
Translation
Position Chain Variation Link
5 5 a, g dbSNP:78460739
24 24 a, t dbSNP:549771908
31 31 -, at dbSNP:375238004
47 47 a, c dbSNP:771131237
64 64 a, g dbSNP:749480182
67 67 a, g dbSNP:778198013
78 78 a, g dbSNP:756496579
80 80 c, g, t dbSNP:781773909
85 85 a, g dbSNP:61754178
95 95 a, g dbSNP:751060367
98 98 c, t dbSNP:765820179
99 99 a, g dbSNP:762599369
100 100 g, t dbSNP:752745494
103 103 a, g dbSNP:750108089
112 112 a, g dbSNP:765061007
115 115 a, c dbSNP:112594248
117 117 a, g dbSNP:776442513
120 120 a, g dbSNP:768531855
129 129 a, g dbSNP:760623120
131 131 a, g dbSNP:774425802
134 134 c, t dbSNP:770985771
141 141 a, g dbSNP:567931151
142 142 c, g dbSNP:777902756
144 144 a, g dbSNP:141846970
149 149 a, c dbSNP:748480175
156 156 a, c dbSNP:113429723
159 159 c, t dbSNP:781703932
169 169 c, g dbSNP:77759270
176 176 a, g dbSNP:775350065
177 177 a, g dbSNP:766463243
186 186 c, g dbSNP:762979463
203 203 a, g dbSNP:773370348
204 204 c, t dbSNP:770087502
223 223 a, g dbSNP:748390461
226 226 g, t dbSNP:377513734
227 227 c, t dbSNP:568438975
231 231 g, t dbSNP:149899785
236 236 a, t dbSNP:747536329
238 238 a, g dbSNP:780467691
246 246 c, t dbSNP:761183996
247 247 a, g dbSNP:745314265
252 252 c, t dbSNP:552799044
253 253 a, g dbSNP:756840167
254 254 a, g dbSNP:753558493
258 258 c, t dbSNP:576734742
259 259 a, g dbSNP:755955503
260 260 a, c dbSNP:752645542
272 272 a, g dbSNP:767315925
279 279 c, g dbSNP:759536569
288 288 a, g dbSNP:773103941
291 291 c, t dbSNP:373534760
292 292 -, g dbSNP:769696107
295 295 a, g, t dbSNP:370085865
299 299 g, t dbSNP:760965778
304 304 c, t dbSNP:775650699
310 310 a, t dbSNP:772557660
328 328 a, g dbSNP:759951895
333 333 a, g dbSNP:774890561
334 334 a, g dbSNP:199524645
338 338 a, c dbSNP:770400357
345 345 c, t dbSNP:538546425
370 370 a, g dbSNP:777284700
372 372 c, g, t dbSNP:143124556
375 375 a, g dbSNP:780843854
379 379 a, c dbSNP:754860343
399 399 a, g dbSNP:549352854
411 411 c, t dbSNP:562528833
421 421 a, g dbSNP:34431503
426 426 a, c dbSNP:752833089
429 429 a, g dbSNP:374243206
434 434 c, t dbSNP:755211714
442 442 g, t dbSNP:560843913
452 452 c, t dbSNP:778942509
467 467 a, g dbSNP:763442293
473 473 a, g dbSNP:139401611
475 475 c, t dbSNP:764631120
499 499 a, g dbSNP:761424749
504 504 a, g dbSNP:776034976
505 505 g, t dbSNP:768297455
514 514 a, g dbSNP:746664897
530 530 c, g dbSNP:775422299
540 540 -, caga dbSNP:387906866
541 541 a, g, t dbSNP:2230818
568 568 c, t dbSNP:774040338
573 573 a, g dbSNP:770840827
581 581 g, t dbSNP:142946965
589 589 a, c dbSNP:373192089
591 591 a, g dbSNP:755105372
599 599 g, t dbSNP:200778881
603 603 g, t dbSNP:780293025
605 605 a, g dbSNP:758650568
606 606 c, t dbSNP:750750076
608 608 c, t dbSNP:548923032
609 609 a, g, t dbSNP:757777916
633 633 c, t dbSNP:768704961
634 634 c, t dbSNP:754362767
639 639 c, t dbSNP:763677117
653 653 c, t dbSNP:760305057
675 675 -, aac dbSNP:777536319
680 680 c, t dbSNP:752288692
682 682 a, g dbSNP:369378891
714 714 c, t dbSNP:146893995
725 725 a, g dbSNP:778386605
729 729 c, t dbSNP:199651827
744 744 c, t dbSNP:200972600
747 747 a, c, g dbSNP:759217858
768 768 a, g dbSNP:751225336
779 779 a, c dbSNP:766171023
785 785 a, g dbSNP:375162225
792 792 c, t dbSNP:536745843
805 805 c, g dbSNP:773704430
810 810 a, g dbSNP:770336510
815 815 c, t dbSNP:748641439
817 817 a, g dbSNP:781586782
824 824 a, g dbSNP:570630045
840 840 a, g dbSNP:371791651
846 846 g, t dbSNP:746453821
865 865 c, g dbSNP:200988113
876 876 c, t dbSNP:201786766
877 877 a, g dbSNP:201123474
882 882 a, g dbSNP:757922213
884 884 -, t dbSNP:769140844
885 885 a, g dbSNP:367951580
914 914 c, t dbSNP:747554740
917 917 a, g dbSNP:779424893
923 923 c, t dbSNP:771618970
924 924 a, t dbSNP:745489345
929 929 a, c dbSNP:151089669
930 930 a, g dbSNP:756928057
931 931 a, g dbSNP:199788242
960 960 a, g dbSNP:573469509
969 969 c, t dbSNP:753521828
971 971 c, t dbSNP:777632615
978 978 a, t dbSNP:756005596
990 990 g, t dbSNP:752733411
1003 1003 a, c, t dbSNP:148983888
1005 1005 c, t dbSNP:750559090
1011 1011 a, g dbSNP:765505576
1018 1018 c, t dbSNP:201481056
1039 1039 g, t dbSNP:776793975
1043 1043 a, c dbSNP:752542509
1055 1055 a, t dbSNP:781232281
1057 1057 a, g dbSNP:199755899
1063 1063 a, g dbSNP:750470899
1065 1065 a, g dbSNP:765410644
1073 1073 a, g dbSNP:762120954
1080 1080 c, t dbSNP:754147588
1091 1091 c, t dbSNP:764334934
1092 1092 a, g dbSNP:761061102
1098 1098 a, g dbSNP:775742267
1134 1134 c, t dbSNP:201738148
1140 1140 c, g dbSNP:757397345
1155 1155 a, g dbSNP:753895855
1175 1175 c, t dbSNP:374447820
1176 1176 a, t dbSNP:756306371
1187 1187 c, t dbSNP:201789177
1188 1188 a, g dbSNP:767940322
1190 1190 a, g dbSNP:760026389
1199 1199 a, c dbSNP:774892528
1201 1201 c, g dbSNP:765839537
1209 1209 a, c dbSNP:762481146
1212 1212 a, g, t dbSNP:769427627
1236 1236 c, t dbSNP:747757837
1249 1249 a, g dbSNP:776562188
1251 1251 a, t dbSNP:768629467
1254 1254 c, t dbSNP:200399376
1255 1255 a, g dbSNP:780099488
1257 1257 a, g dbSNP:566565696
1263 1263 c, t dbSNP:749333487
1264 1264 a, g dbSNP:777722013
1269 1269 c, t dbSNP:756357298
1270 1270 a, g dbSNP:764909573
1292 1292 a, c dbSNP:748334117
1309 1309 a, g dbSNP:143293318
1313 1313 a, g dbSNP:201873770
1328 1328 a, g dbSNP:146433601
1338 1338 a, g dbSNP:746502094
1350 1350 a, g dbSNP:758941402
1354 1354 c, t dbSNP:750951308
1355 1355 a, g dbSNP:368697823
1358 1358 a, g dbSNP:761448887
1365 1365 a, c dbSNP:753420524
1370 1370 g, t dbSNP:376540653
1379 1379 c, t dbSNP:760407268
1380 1380 a, g dbSNP:144137945
1385 1385 c, t dbSNP:777478676
1408 1408 c, t dbSNP:55937573
1418 1418 c, t dbSNP:771817640
1431 1431 c, t dbSNP:759491821
1432 1432 a, g dbSNP:774191569
1437 1437 c, t dbSNP:770937007
1447 1447 a, g dbSNP:201743292
1448 1448 a, g dbSNP:200446953
1450 1450 a, g dbSNP:372967979
1451 1451 a, g dbSNP:747301065
1457 1457 c, t dbSNP:780454096
1458 1458 a, g dbSNP:758739864
1468 1468 a, g dbSNP:554069834
1516 1516 c, t dbSNP:779339431
1523 1523 c, g dbSNP:372323794
1534 1534 -, aag dbSNP:760760055
1539 1539 c, t dbSNP:757833957
1547 1547 c, t dbSNP:749866093
1548 1548 a, g dbSNP:368127694
1550 1550 c, t dbSNP:777392691
1553 1553 a, g dbSNP:755721769
1558 1558 a, g dbSNP:144014465
1568 1568 c, g dbSNP:375981644
1569 1569 c, t dbSNP:754676488
1571 1571 c, t dbSNP:751249609
1578 1578 c, t dbSNP:147475420
1581 1581 c, t dbSNP:762867130
1586 1586 a, g dbSNP:542316178
1590 1590 g, t dbSNP:754585612
1591 1591 a, g dbSNP:751286148
1611 1611 a, g dbSNP:779804030
1621 1621 a, g dbSNP:549183501
1622 1622 a, g dbSNP:750249923
1632 1632 a, c, t dbSNP:56237316
1633 1633 g, t dbSNP:752714332
1660 1660 c, g dbSNP:767693528
1666 1666 a, g dbSNP:759653644
1667 1667 a, g dbSNP:774751396
1671 1671 c, t dbSNP:771307389
1677 1677 c, t dbSNP:763358232
1680 1680 c, t dbSNP:773721139
1681 1681 c, t dbSNP:770135526
1683 1683 c, t dbSNP:748758847
1684 1684 a, g dbSNP:780557617
1692 1692 a, g dbSNP:768262672
1726 1726 g, t dbSNP:769076594
1761 1761 c, t dbSNP:1048610
1762 1762 a, g dbSNP:779554063
1765 1765 c, t dbSNP:771706819
1766 1766 a, g, t dbSNP:140536552
1770 1770 c, t dbSNP:200055210
1771 1771 a, g dbSNP:749066091
1776 1776 c, t dbSNP:777714532
1783 1783 a, g dbSNP:756129840
1786 1786 g, t dbSNP:751618802
1797 1797 g, t dbSNP:530963981
1804 1804 c, t dbSNP:766635709
1823 1823 c, t dbSNP:758683558
1830 1830 a, g dbSNP:750726481
1831 1831 a, g dbSNP:371598292
1839 1839 c, t dbSNP:762200753
1845 1845 c, t dbSNP:776882539
1867 1867 c, t dbSNP:749050766
1868 1868 a, g dbSNP:777431052
1869 1869 a, g dbSNP:756119780
1872 1872 a, g dbSNP:761422866
1879 1879 a, g dbSNP:748159167
1887 1887 a, g dbSNP:112463036
1891 1891 c, t dbSNP:780262610
1897 1897 a, g dbSNP:758594009
1907 1907 a, g dbSNP:144657795
1911 1911 g, t dbSNP:765452935
1918 1918 a, t dbSNP:757433621
1922 1922 a, g dbSNP:140933944
1931 1931 c, g dbSNP:745903113
1932 1932 a, c, g, t dbSNP:369229801
1934 1934 a, g dbSNP:778078194
1940 1940 c, t dbSNP:756429621
1953 1953 c, t dbSNP:753079319
1954 1954 a, g dbSNP:61754177
1968 1968 a, g dbSNP:759960474
1976 1976 -, cct dbSNP:746768799
2008 2008 a, g dbSNP:751073259
2010 2010 c, t dbSNP:34355677
2015 2015 a, g dbSNP:375942302
2029 2029 c, t dbSNP:576343468
2034 2034 a, t dbSNP:765883520
2041 2041 a, t dbSNP:762334638
2044 2044 c, g dbSNP:749976457
2048 2048 c, t dbSNP:747163792
2050 2050 -, ct dbSNP:767985722
2052 2052 a, g dbSNP:764926423
2055 2055 c, t dbSNP:370157347
2056 2056 -, ct dbSNP:760143401
2056 2056 c, t dbSNP:776564382
2062 2062 c, t dbSNP:778028713
2064 2064 c, t dbSNP:768383645
2073 2073 a, c, t dbSNP:775368008
2076 2076 c, t dbSNP:767287511
2077 2077 a, g dbSNP:369159078
2085 2085 a, g dbSNP:773264235
2091 2091 c, t dbSNP:202041986
2105 2105 c, g dbSNP:144721235
2110 2110 c, t dbSNP:748342054
2111 2111 a, g dbSNP:147275585
2123 2123 a, c dbSNP:768949233
2125 2125 c, t dbSNP:372189309
2132 2132 c, t dbSNP:181463078
2133 2133 a, g, t dbSNP:746436310
2136 2136 c, g dbSNP:148093044
2142 2142 c, g, t dbSNP:374390369
2143 2143 c, g dbSNP:763595824
2148 2148 c, t dbSNP:755792086
2149 2149 c, t dbSNP:370064783
2150 2150 a, g dbSNP:538865826
2151 2151 c, t dbSNP:759443841
2155 2155 a, c dbSNP:765817788
2156 2156 a, c dbSNP:774314959
2157 2157 a, g dbSNP:766337117
2160 2160 c, t dbSNP:200268337
2161 2161 a, g dbSNP:761836949
2177 2177 c, t dbSNP:55796712
2178 2178 a, g, t dbSNP:376456430
2179 2179 a, g dbSNP:761025971
2180 2180 c, t dbSNP:79932015
2185 2185 a, g dbSNP:374624230
2188 2188 g, t dbSNP:772616091
2198 2198 a, t dbSNP:746264614
2199 2199 c, g dbSNP:370037406
2207 2207 a, g dbSNP:779511668
2213 2213 c, t dbSNP:771579137
2216 2216 a, c dbSNP:748739807
2217 2217 c, t dbSNP:371018540
2219 2219 c, g dbSNP:755704723
2230 2230 a, c, g dbSNP:780815372
2233 2233 c, g dbSNP:754730403
2237 2237 a, g dbSNP:751381392
2240 2240 -, ca dbSNP:768475497
2245 2245 -, tc dbSNP:746900439
2253 2253 c, g dbSNP:766251160
2256 2256 c, t dbSNP:376241260
2257 2257 a, g dbSNP:753859098
2263 2263 a, g dbSNP:764293730
2264 2264 a, g dbSNP:760787134
2273 2273 a, g dbSNP:775739935
2277 2277 a, c dbSNP:772443121
2279 2279 a, c dbSNP:759921163
2280 2280 -, c dbSNP:779871088
2283 2283 c, t dbSNP:201360504
2285 2285 a, c dbSNP:78855175
2293 2293 a, g dbSNP:771710782
2316 2316 g, t dbSNP:771556813
2317 2317 a, g dbSNP:749860291
2324 2324 c, t dbSNP:572250428
2325 2325 a, g dbSNP:149361049
2333 2333 c, t dbSNP:747645870
2335 2335 g, t dbSNP:780850654
2352 2352 -, ggctgcctcctt dbSNP:771854768
2353 2353 a, c, g, t dbSNP:761391373
2360 2360 c, t dbSNP:758318148
2372 2372 a, g dbSNP:750426388
2374 2374 c, t dbSNP:764050476
2375 2375 a, g dbSNP:760757602
2383 2383 c, t dbSNP:752730764
2384 2384 a, g, t dbSNP:754284338
2389 2389 c, g dbSNP:142315365
2391 2391 -, cagcaaaga dbSNP:777944947
2403 2403 -, a dbSNP:756372734
2405 2405 -, ag dbSNP:781314197
2420 2420 c, t dbSNP:766895883
2422 2422 c, t dbSNP:763335447
2437 2437 -, ctta dbSNP:752044573
2438 2438 a, c dbSNP:773726116
2443 2443 c, g dbSNP:371014633
2445 2445 a, c dbSNP:747638598
2469 2469 -, acaaa dbSNP:756905441
2471 2471 -, a dbSNP:397871862
2473 2473 -, atcaa dbSNP:374487688
2473 2473 -, a dbSNP:3833563
2477 2477 a, g dbSNP:547401766
2487 2487 a, g dbSNP:6705408
2543 2543 c, t dbSNP:779832226
2608 2608 a, g dbSNP:377317204
2639 2639 c, g dbSNP:551774656
2695 2695 g, t dbSNP:769827227
2702 2702 a, g dbSNP:1130094
2765 2765 -, t dbSNP:5829218
2765 2765 g, t dbSNP:551456834
2774 2774 -, t dbSNP:111759422
2774 2774 a, t dbSNP:531849283
2792 2792 a, g dbSNP:73149881
2801 2801 a, g dbSNP:549261463
2816 2816 c, t dbSNP:529615832
2837 2837 -, tagt dbSNP:77130641
2843 2843 -, g dbSNP:550667141
2844 2844 g, t dbSNP:189060518
2858 2858 -, tttttt dbSNP:775488489
2858 2858 -, ttttt dbSNP:749119807
2858 2858 -, tttt dbSNP:774018291
2860 2860 -, tttt dbSNP:548548539

Target ORF information:

RefSeq Version XM_011510375
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ADAM metallopeptidase domain 17 (ADAM17), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011510375

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGAAGAAGC TTGATTCTTT GCTCTCAGAC TACGATATTC TCTCTTTATC TAATATCCAG 
CAGCATTCGG TAAGAAAAAG AGATCTACAG ACTTCAACAC ATGTAGAAAC ACTACTAACT
TTTTCAGCTT TGAAAAGGCA TTTTAAATTA TACCTGACAT CAAGTACTGA ACGTTTTTCA
CAAAATTTCA AGGTCGTGGT GGTGGATGGT AAAAACGAAA GCGAGTACAC TGTAAAATGG
CAGGACTTCT TCACTGGACA CGTGGTTGGT GAGCCTGACT CTAGGGTTCT AGCCCACATA
AGAGATGATG ATGTTATAAT CAGAATCAAC ACAGATGGGG CCGAATATAA CATAGAGCCA
CTTTGGAGAT TTGTTAATGA TACCAAAGAC AAAAGAATGT TAGTTTATAA ATCTGAAGAT
ATCAAGAATG TTTCACGTTT GCAGTCTCCA AAAGTGTGTG GTTATTTAAA AGTGGATAAT
GAAGAGTTGC TCCCAAAAGG GTTAGTAGAC AGAGAACCAC CTGAAGAGCT TGTTCATCGA
GTGAAAAGAA GAGCTGACCC AGATCCCATG AAGAACACGT GTAAATTATT GGTGGTAGCA
GATCATCGCT TCTACAGATA CATGGGCAGA GGGGAAGAGA GTACAACTAC AAATTACTTA
ATAGAGCTAA TTGACAGAGT TGATGACATC TATCGGAACA CTTCATGGGA TAATGCAGGT
TTTAAAGGCT ATGGAATACA GATAGAGCAG ATTCGCATTC TCAAGTCTCC ACAAGAGGTA
AAACCTGGTG AAAAGCACTA CAACATGGCA AAAAGTTACC CAAATGAAGA AAAGGATGCT
TGGGATGTGA AGATGTTGCT AGAGCAATTT AGCTTTGATA TAGCTGAGGA AGCATCTAAA
GTTTGCTTGG CACACCTTTT CACATACCAA GATTTTGATA TGGGAACTCT TGGATTAGCT
TATGTTGGCT CTCCCAGAGC AAACAGCCAT GGAGGTGTTT GTCCAAAGGC TTATTATAGC
CCAGTTGGGA AGAAAAATAT CTATTTGAAT AGTGGTTTGA CGAGCACAAA GAATTATGGT
AAAACCATCC TTACAAAGGA AGCTGACCTG GTTACAACTC ATGAATTGGG ACATAATTTT
GGAGCAGAAC ATGATCCGGA TGGTCTAGCA GAATGTGCCC CGAATGAGGA CCAGGGAGGG
AAATATGTCA TGTATCCCAT AGCTGTGAGT GGCGATCACG AGAACAATAA GATGTTTTCA
AACTGCAGTA AACAATCAAT CTATAAGACC ATTGAAAGTA AGGCCCAGGA GTGTTTTCAA
GAACGCAGCA ATAAAGTTTG TGGGAACTCG AGGGTGGATG AAGGAGAAGA GTGTGATCCT
GGCATCATGT ATCTGAACAA CGACACCTGC TGCAACAGCG ACTGCACGTT GAAGGAAGGT
GTCCAGTGCA GTGACAGGAA CAGTCCTTGC TGTAAAAACT GTCAGTTTGA GACTGCCCAG
AAGAAGTGCC AGGAGGCGAT TAATGCTACT TGCAAAGGCG TGTCCTACTG CACAGGTAAT
AGCAGTGAGT GCCCGCCTCC AGGAAATGCT GAAGATGACA CTGTTTGCTT GGATCTTGGC
AAGTGTAAGG ATGGGAAATG CATCCCTTTC TGCGAGAGGG AACAGCAGCT GGAGTCCTGT
GCATGTAATG AAACTGACAA CTCCTGCAAG GTGTGCTGCA GGGACCTTTC TGGCCGCTGT
GTGCCCTATG TCGATGCTGA ACAAAAGAAC TTATTTTTGA GGAAAGGAAA GCCCTGTACA
GTAGGATTTT GTGACATGAA TGGCAAATGT GAGAAACGAG TACAGGATGT AATTGAACGA
TTTTGGGATT TCATTGACCA GCTGAGCATC AATACTTTTG GAAAGTTTTT AGCAGACAAC
ATCGTTGGGT CTGTCCTGGT TTTCTCCTTG ATATTTTGGA TTCCTTTCAG CATTCTTGTC
CATTGTGTGG ATAAGAAATT GGATAAACAG TATGAATCTC TGTCTCTGTT TCACCCCAGT
AACGTCGAAA TGCTGAGCAG CATGGATTCT GCATCGGTTC GCATTATCAA ACCCTTTCCT
GCGCCCCAGA CTCCAGGCCG CCTGCAGCCT GCCCCTGTGA TCCCTTCGGC GCCAGCAGCT
CCAAAACTGG ACCACCAGAG AATGGACACC ATCCAGGAAG ACCCCAGCAC AGACTCACAT
ATGGACGAGG ATGGGTTTGA GAAGGACCCC TTCCCAAATA GCAGCACAGC TGCCAAGTCA
TTTGAGGATC TCACGGACCA TCCGGTCACC AGAAGTGAAA AGGCTGCCTC CTTTAAACTG
CAGCGTCAGA ATCGTGTTGA CAGCAAAGAA ACAGAGTGCTT AA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu60844D
Sequence Information ORF Nucleotide Sequence (Length: 1815bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product disintegrin and metalloproteinase domain-containing protein 17 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005334.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGAAGAACA CGTGTAAATT ATTGGTGGTA GCAGATCATC GCTTCTACAG ATACATGGGC 
AGAGGGGAAG AGAGTACAAC TACAAATTAC TTAATAGAGC TAATTGACAG AGTTGATGAC
ATCTATCGGA ACACTTCATG GGATAATGCA GGTTTTAAAG GCTATGGAAT ACAGATAGAG
CAGATTCGCA TTCTCAAGTC TCCACAAGAG GTAAAACCTG GTGAAAAGCA CTACAACATG
GCAAAAAGTT ACCCAAATGA AGAAAAGGAT GCTTGGGATG TGAAGATGTT GCTAGAGCAA
TTTAGCTTTG ATATAGCTGA GGAAGCATCT AAAGTTTGCT TGGCACACCT TTTCACATAC
CAAGATTTTG ATATGGGAAC TCTTGGATTA GCTTATGTTG GCTCTCCCAG AGCAAACAGC
CATGGAGGTG TTTGTCCAAA GGCTTATTAT AGCCCAGTTG GGAAGAAAAA TATCTATTTG
AATAGTGGTT TGACGAGCAC AAAGAATTAT GGTAAAACCA TCCTTACAAA GGAAGCTGAC
CTGGTTACAA CTCATGAATT GGGACATAAT TTTGGAGCAG AACATGATCC GGATGGTCTA
GCAGAATGTG CCCCGAATGA GGACCAGGGA GGGAAATATG TCATGTATCC CATAGCTGTG
AGTGGCGATC ACGAGAACAA TAAGATGTTT TCAAACTGCA GTAAACAATC AATCTATAAG
ACCATTGAAA GTAAGGCCCA GGAGTGTTTT CAAGAACGCA GCAATAAAGT TTGTGGGAAC
TCGAGGGTGG ATGAAGGAGA AGAGTGTGAT CCTGGCATCA TGTATCTGAA CAACGACACC
TGCTGCAACA GCGACTGCAC GTTGAAGGAA GGTGTCCAGT GCAGTGACAG GAACAGTCCT
TGCTGTAAAA ACTGTCAGTT TGAGACTGCC CAGAAGAAGT GCCAGGAGGC GATTAATGCT
ACTTGCAAAG GCGTGTCCTA CTGCACAGGT AATAGCAGTG AGTGCCCGCC TCCAGGAAAT
GCTGAAGATG ACACTGTTTG CTTGGATCTT GGCAAGTGTA AGGATGGGAA ATGCATCCCT
TTCTGCGAGA GGGAACAGCA GCTGGAGTCC TGTGCATGTA ATGAAACTGA CAACTCCTGC
AAGGTGTGCT GCAGGGACCT TTCTGGCCGC TGTGTGCCCT ATGTCGATGC TGAACAAAAG
AACTTATTTT TGAGGAAAGG AAAGCCCTGT ACAGTAGGAT TTTGTGACAT GAATGGCAAA
TGTGAGAAAC GAGTACAGGA TGTAATTGAA CGATTTTGGG ATTTCATTGA CCAGCTGAGC
ATCAATACTT TTGGAAAGTT TTTAGCAGAC AACATCGTTG GGTCTGTCCT GGTTTTCTCC
TTGATATTTT GGATTCCTTT CAGCATTCTT GTCCATTGTG TGGATAAGAA ATTGGATAAA
CAGTATGAAT CTCTGTCTCT GTTTCACCCC AGTAACGTCG AAATGCTGAG CAGCATGGAT
TCTGCATCGG TTCGCATTAT CAAACCCTTT CCTGCGCCCC AGACTCCAGG CCGCCTGCAG
CCTGCCCCTG TGATCCCTTC GGCGCCAGCA GCTCCAAAAC TGGACCACCA GAGAATGGAC
ACCATCCAGG AAGACCCCAG CACAGACTCA CATATGGACG AGGATGGGTT TGAGAAGGAC
CCCTTCCCAA ATAGCAGCAC AGCTGCCAAG TCATTTGAGG ATCTCACGGA CCATCCGGTC
ACCAGAAGTG AAAAGGCTGC CTCCTTTAAA CTGCAGCGTC AGAATCGTGT TGACAGCAAA
GAAACAGAGT GCTAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011508678.1
CDS188..2002
Misc Feature(1)194..958(+)
Misc Feature(2)740..772(+)
Misc Feature(3)977..1207(+)
Misc Feature(4)1265..1453(+)
Misc Feature(5)1325..1432(+)
Misc Feature(6)1325..1336(+)
Translation
Position Chain Variation Link
4 4 c, t dbSNP:755211714
12 12 g, t dbSNP:560843913
22 22 c, t dbSNP:778942509
37 37 a, g dbSNP:763442293
43 43 a, g dbSNP:139401611
45 45 c, t dbSNP:764631120
69 69 a, g dbSNP:761424749
74 74 a, g dbSNP:776034976
75 75 g, t dbSNP:768297455
84 84 a, g dbSNP:746664897
100 100 c, g dbSNP:775422299
110 110 -, caga dbSNP:387906866
111 111 a, g, t dbSNP:2230818
130 130 a, g dbSNP:745623517
138 138 -, t dbSNP:753415769
158 158 c, t dbSNP:774040338
163 163 a, g dbSNP:770840827
171 171 g, t dbSNP:142946965
179 179 a, c dbSNP:373192089
181 181 a, g dbSNP:755105372
189 189 g, t dbSNP:200778881
193 193 g, t dbSNP:780293025
195 195 a, g dbSNP:758650568
196 196 c, t dbSNP:750750076
198 198 c, t dbSNP:548923032
199 199 a, g, t dbSNP:757777916
223 223 c, t dbSNP:768704961
224 224 c, t dbSNP:754362767
229 229 c, t dbSNP:763677117
243 243 c, t dbSNP:760305057
265 265 -, aac dbSNP:777536319
270 270 c, t dbSNP:752288692
272 272 a, g dbSNP:369378891
304 304 c, t dbSNP:146893995
315 315 a, g dbSNP:778386605
319 319 c, t dbSNP:199651827
334 334 c, t dbSNP:200972600
337 337 a, c, g dbSNP:759217858
358 358 a, g dbSNP:751225336
369 369 a, c dbSNP:766171023
375 375 a, g dbSNP:375162225
382 382 c, t dbSNP:536745843
395 395 c, g dbSNP:773704430
400 400 a, g dbSNP:770336510
405 405 c, t dbSNP:748641439
407 407 a, g dbSNP:781586782
414 414 a, g dbSNP:570630045
430 430 a, g dbSNP:371791651
436 436 g, t dbSNP:746453821
455 455 c, g dbSNP:200988113
466 466 c, t dbSNP:201786766
467 467 a, g dbSNP:201123474
472 472 a, g dbSNP:757922213
474 474 -, t dbSNP:769140844
475 475 a, g dbSNP:367951580
504 504 c, t dbSNP:747554740
507 507 a, g dbSNP:779424893
513 513 c, t dbSNP:771618970
514 514 a, t dbSNP:745489345
519 519 a, c dbSNP:151089669
520 520 a, g dbSNP:756928057
521 521 a, g dbSNP:199788242
550 550 a, g dbSNP:573469509
559 559 c, t dbSNP:753521828
561 561 c, t dbSNP:777632615
568 568 a, t dbSNP:756005596
580 580 g, t dbSNP:752733411
593 593 a, c, t dbSNP:148983888
595 595 c, t dbSNP:750559090
601 601 a, g dbSNP:765505576
608 608 c, t dbSNP:201481056
629 629 g, t dbSNP:776793975
633 633 a, c dbSNP:752542509
645 645 a, t dbSNP:781232281
647 647 a, g dbSNP:199755899
653 653 a, g dbSNP:750470899
655 655 a, g dbSNP:765410644
663 663 a, g dbSNP:762120954
670 670 c, t dbSNP:754147588
681 681 c, t dbSNP:764334934
682 682 a, g dbSNP:761061102
688 688 a, g dbSNP:775742267
724 724 c, t dbSNP:201738148
730 730 c, g dbSNP:757397345
745 745 a, g dbSNP:753895855
765 765 c, t dbSNP:374447820
766 766 a, t dbSNP:756306371
777 777 c, t dbSNP:201789177
778 778 a, g dbSNP:767940322
780 780 a, g dbSNP:760026389
789 789 a, c dbSNP:774892528
791 791 c, g dbSNP:765839537
799 799 a, c dbSNP:762481146
802 802 a, g, t dbSNP:769427627
826 826 c, t dbSNP:747757837
839 839 a, g dbSNP:776562188
841 841 a, t dbSNP:768629467
844 844 c, t dbSNP:200399376
845 845 a, g dbSNP:780099488
847 847 a, g dbSNP:566565696
853 853 c, t dbSNP:749333487
854 854 a, g dbSNP:777722013
859 859 c, t dbSNP:756357298
860 860 a, g dbSNP:764909573
882 882 a, c dbSNP:748334117
899 899 a, g dbSNP:143293318
903 903 a, g dbSNP:201873770
918 918 a, g dbSNP:146433601
928 928 a, g dbSNP:746502094
940 940 a, g dbSNP:758941402
944 944 c, t dbSNP:750951308
945 945 a, g dbSNP:368697823
948 948 a, g dbSNP:761448887
955 955 a, c dbSNP:753420524
960 960 g, t dbSNP:376540653
969 969 c, t dbSNP:760407268
970 970 a, g dbSNP:144137945
975 975 c, t dbSNP:777478676
998 998 c, t dbSNP:55937573
1008 1008 c, t dbSNP:771817640
1021 1021 c, t dbSNP:759491821
1022 1022 a, g dbSNP:774191569
1027 1027 c, t dbSNP:770937007
1037 1037 a, g dbSNP:201743292
1038 1038 a, g dbSNP:200446953
1040 1040 a, g dbSNP:372967979
1041 1041 a, g dbSNP:747301065
1047 1047 c, t dbSNP:780454096
1048 1048 a, g dbSNP:758739864
1058 1058 a, g dbSNP:554069834
1106 1106 c, t dbSNP:779339431
1113 1113 c, g dbSNP:372323794
1124 1124 -, aag dbSNP:760760055
1129 1129 c, t dbSNP:757833957
1137 1137 c, t dbSNP:749866093
1138 1138 a, g dbSNP:368127694
1140 1140 c, t dbSNP:777392691
1143 1143 a, g dbSNP:755721769
1148 1148 a, g dbSNP:144014465
1158 1158 c, g dbSNP:375981644
1159 1159 c, t dbSNP:754676488
1161 1161 c, t dbSNP:751249609
1168 1168 c, t dbSNP:147475420
1171 1171 c, t dbSNP:762867130
1176 1176 a, g dbSNP:542316178
1180 1180 g, t dbSNP:754585612
1181 1181 a, g dbSNP:751286148
1201 1201 a, g dbSNP:779804030
1211 1211 a, g dbSNP:549183501
1212 1212 a, g dbSNP:750249923
1222 1222 a, c, t dbSNP:56237316
1223 1223 g, t dbSNP:752714332
1250 1250 c, g dbSNP:767693528
1256 1256 a, g dbSNP:759653644
1257 1257 a, g dbSNP:774751396
1261 1261 c, t dbSNP:771307389
1267 1267 c, t dbSNP:763358232
1270 1270 c, t dbSNP:773721139
1271 1271 c, t dbSNP:770135526
1273 1273 c, t dbSNP:748758847
1274 1274 a, g dbSNP:780557617
1282 1282 a, g dbSNP:768262672
1316 1316 g, t dbSNP:769076594
1351 1351 c, t dbSNP:1048610
1352 1352 a, g dbSNP:779554063
1355 1355 c, t dbSNP:771706819
1356 1356 a, g, t dbSNP:140536552
1360 1360 c, t dbSNP:200055210
1361 1361 a, g dbSNP:749066091
1366 1366 c, t dbSNP:777714532
1373 1373 a, g dbSNP:756129840
1376 1376 g, t dbSNP:751618802
1387 1387 g, t dbSNP:530963981
1394 1394 c, t dbSNP:766635709
1413 1413 c, t dbSNP:758683558
1420 1420 a, g dbSNP:750726481
1421 1421 a, g dbSNP:371598292
1429 1429 c, t dbSNP:762200753
1435 1435 c, t dbSNP:776882539
1457 1457 c, t dbSNP:749050766
1458 1458 a, g dbSNP:777431052
1459 1459 a, g dbSNP:756119780
1462 1462 a, g dbSNP:761422866
1469 1469 a, g dbSNP:748159167
1477 1477 a, g dbSNP:112463036
1481 1481 c, t dbSNP:780262610
1487 1487 a, g dbSNP:758594009
1497 1497 a, g dbSNP:144657795
1501 1501 g, t dbSNP:765452935
1508 1508 a, t dbSNP:757433621
1512 1512 a, g dbSNP:140933944
1521 1521 c, g dbSNP:745903113
1522 1522 a, c, g, t dbSNP:369229801
1524 1524 a, g dbSNP:778078194
1530 1530 c, t dbSNP:756429621
1543 1543 c, t dbSNP:753079319
1544 1544 a, g dbSNP:61754177
1558 1558 a, g dbSNP:759960474
1566 1566 -, cct dbSNP:746768799
1598 1598 a, g dbSNP:751073259
1600 1600 c, t dbSNP:34355677
1605 1605 a, g dbSNP:375942302
1619 1619 c, t dbSNP:576343468
1624 1624 a, t dbSNP:765883520
1631 1631 a, t dbSNP:762334638
1634 1634 c, g dbSNP:749976457
1638 1638 c, t dbSNP:747163792
1640 1640 -, ct dbSNP:767985722
1642 1642 a, g dbSNP:764926423
1645 1645 c, t dbSNP:370157347
1646 1646 -, ct dbSNP:760143401
1646 1646 c, t dbSNP:776564382
1652 1652 c, t dbSNP:778028713
1654 1654 c, t dbSNP:768383645
1663 1663 a, c, t dbSNP:775368008
1666 1666 c, t dbSNP:767287511
1667 1667 a, g dbSNP:369159078
1675 1675 a, g dbSNP:773264235
1681 1681 c, t dbSNP:202041986
1695 1695 c, g dbSNP:144721235
1700 1700 c, t dbSNP:748342054
1701 1701 a, g dbSNP:147275585
1713 1713 a, c dbSNP:768949233
1715 1715 c, t dbSNP:372189309
1722 1722 c, t dbSNP:181463078
1723 1723 a, g, t dbSNP:746436310
1726 1726 c, g dbSNP:148093044
1732 1732 c, g, t dbSNP:374390369
1733 1733 c, g dbSNP:763595824
1738 1738 c, t dbSNP:755792086
1739 1739 c, t dbSNP:370064783
1740 1740 a, g dbSNP:538865826
1741 1741 c, t dbSNP:759443841
1745 1745 a, c dbSNP:765817788
1746 1746 a, c dbSNP:774314959
1747 1747 a, g dbSNP:766337117
1750 1750 c, t dbSNP:200268337
1751 1751 a, g dbSNP:761836949
1767 1767 c, t dbSNP:55796712
1768 1768 a, g, t dbSNP:376456430
1769 1769 a, g dbSNP:761025971
1770 1770 c, t dbSNP:79932015
1775 1775 a, g dbSNP:374624230
1778 1778 g, t dbSNP:772616091
1788 1788 a, t dbSNP:746264614
1789 1789 c, g dbSNP:370037406
1797 1797 a, g dbSNP:779511668
1803 1803 c, t dbSNP:771579137
1806 1806 a, c dbSNP:748739807
1807 1807 c, t dbSNP:371018540
1809 1809 c, g dbSNP:755704723
1820 1820 a, c, g dbSNP:780815372
1823 1823 c, g dbSNP:754730403
1827 1827 a, g dbSNP:751381392
1830 1830 -, ca dbSNP:768475497
1835 1835 -, tc dbSNP:746900439
1843 1843 c, g dbSNP:766251160
1846 1846 c, t dbSNP:376241260
1847 1847 a, g dbSNP:753859098
1853 1853 a, g dbSNP:764293730
1854 1854 a, g dbSNP:760787134
1863 1863 a, g dbSNP:775739935
1867 1867 a, c dbSNP:772443121
1869 1869 a, c dbSNP:759921163
1870 1870 -, c dbSNP:779871088
1873 1873 c, t dbSNP:201360504
1875 1875 a, c dbSNP:78855175
1883 1883 a, g dbSNP:771710782
1906 1906 g, t dbSNP:771556813
1907 1907 a, g dbSNP:749860291
1914 1914 c, t dbSNP:572250428
1915 1915 a, g dbSNP:149361049
1923 1923 c, t dbSNP:747645870
1925 1925 g, t dbSNP:780850654
1942 1942 -, ggctgcctcctt dbSNP:771854768
1943 1943 a, c, g, t dbSNP:761391373
1950 1950 c, t dbSNP:758318148
1962 1962 a, g dbSNP:750426388
1964 1964 c, t dbSNP:764050476
1965 1965 a, g dbSNP:760757602
1973 1973 c, t dbSNP:752730764
1974 1974 a, g, t dbSNP:754284338
1979 1979 c, g dbSNP:142315365
1981 1981 -, cagcaaaga dbSNP:777944947
1993 1993 -, a dbSNP:756372734
1995 1995 -, ag dbSNP:781314197
2010 2010 c, t dbSNP:766895883
2012 2012 c, t dbSNP:763335447
2027 2027 -, ctta dbSNP:752044573
2028 2028 a, c dbSNP:773726116
2033 2033 c, g dbSNP:371014633
2035 2035 a, c dbSNP:747638598
2059 2059 -, acaaa dbSNP:756905441
2061 2061 -, a dbSNP:397871862
2063 2063 -, atcaa dbSNP:374487688
2063 2063 -, a dbSNP:3833563
2067 2067 a, g dbSNP:547401766
2077 2077 a, g dbSNP:6705408
2133 2133 c, t dbSNP:779832226
2198 2198 a, g dbSNP:377317204
2229 2229 c, g dbSNP:551774656
2285 2285 g, t dbSNP:769827227
2292 2292 a, g dbSNP:1130094
2355 2355 -, t dbSNP:5829218
2355 2355 g, t dbSNP:551456834
2364 2364 -, t dbSNP:111759422
2364 2364 a, t dbSNP:531849283
2382 2382 a, g dbSNP:73149881
2391 2391 a, g dbSNP:549261463
2406 2406 c, t dbSNP:529615832
2427 2427 -, tagt dbSNP:77130641
2433 2433 -, g dbSNP:550667141
2434 2434 g, t dbSNP:189060518
2448 2448 -, tttttt dbSNP:775488489
2448 2448 -, ttttt dbSNP:749119807
2448 2448 -, tttt dbSNP:774018291
2450 2450 -, tttt dbSNP:548548539

Target ORF information:

RefSeq Version XM_011510376
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ADAM metallopeptidase domain 17 (ADAM17), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011510376

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGAAGAACA CGTGTAAATT ATTGGTGGTA GCAGATCATC GCTTCTACAG ATACATGGGC 
AGAGGGGAAG AGAGTACAAC TACAAATTAC TTAATAGAGC TAATTGACAG AGTTGATGAC
ATCTATCGGA ACACTTCATG GGATAATGCA GGTTTTAAAG GCTATGGAAT ACAGATAGAG
CAGATTCGCA TTCTCAAGTC TCCACAAGAG GTAAAACCTG GTGAAAAGCA CTACAACATG
GCAAAAAGTT ACCCAAATGA AGAAAAGGAT GCTTGGGATG TGAAGATGTT GCTAGAGCAA
TTTAGCTTTG ATATAGCTGA GGAAGCATCT AAAGTTTGCT TGGCACACCT TTTCACATAC
CAAGATTTTG ATATGGGAAC TCTTGGATTA GCTTATGTTG GCTCTCCCAG AGCAAACAGC
CATGGAGGTG TTTGTCCAAA GGCTTATTAT AGCCCAGTTG GGAAGAAAAA TATCTATTTG
AATAGTGGTT TGACGAGCAC AAAGAATTAT GGTAAAACCA TCCTTACAAA GGAAGCTGAC
CTGGTTACAA CTCATGAATT GGGACATAAT TTTGGAGCAG AACATGATCC GGATGGTCTA
GCAGAATGTG CCCCGAATGA GGACCAGGGA GGGAAATATG TCATGTATCC CATAGCTGTG
AGTGGCGATC ACGAGAACAA TAAGATGTTT TCAAACTGCA GTAAACAATC AATCTATAAG
ACCATTGAAA GTAAGGCCCA GGAGTGTTTT CAAGAACGCA GCAATAAAGT TTGTGGGAAC
TCGAGGGTGG ATGAAGGAGA AGAGTGTGAT CCTGGCATCA TGTATCTGAA CAACGACACC
TGCTGCAACA GCGACTGCAC GTTGAAGGAA GGTGTCCAGT GCAGTGACAG GAACAGTCCT
TGCTGTAAAA ACTGTCAGTT TGAGACTGCC CAGAAGAAGT GCCAGGAGGC GATTAATGCT
ACTTGCAAAG GCGTGTCCTA CTGCACAGGT AATAGCAGTG AGTGCCCGCC TCCAGGAAAT
GCTGAAGATG ACACTGTTTG CTTGGATCTT GGCAAGTGTA AGGATGGGAA ATGCATCCCT
TTCTGCGAGA GGGAACAGCA GCTGGAGTCC TGTGCATGTA ATGAAACTGA CAACTCCTGC
AAGGTGTGCT GCAGGGACCT TTCTGGCCGC TGTGTGCCCT ATGTCGATGC TGAACAAAAG
AACTTATTTT TGAGGAAAGG AAAGCCCTGT ACAGTAGGAT TTTGTGACAT GAATGGCAAA
TGTGAGAAAC GAGTACAGGA TGTAATTGAA CGATTTTGGG ATTTCATTGA CCAGCTGAGC
ATCAATACTT TTGGAAAGTT TTTAGCAGAC AACATCGTTG GGTCTGTCCT GGTTTTCTCC
TTGATATTTT GGATTCCTTT CAGCATTCTT GTCCATTGTG TGGATAAGAA ATTGGATAAA
CAGTATGAAT CTCTGTCTCT GTTTCACCCC AGTAACGTCG AAATGCTGAG CAGCATGGAT
TCTGCATCGG TTCGCATTAT CAAACCCTTT CCTGCGCCCC AGACTCCAGG CCGCCTGCAG
CCTGCCCCTG TGATCCCTTC GGCGCCAGCA GCTCCAAAAC TGGACCACCA GAGAATGGAC
ACCATCCAGG AAGACCCCAG CACAGACTCA CATATGGACG AGGATGGGTT TGAGAAGGAC
CCCTTCCCAA ATAGCAGCAC AGCTGCCAAG TCATTTGAGG ATCTCACGGA CCATCCGGTC
ACCAGAAGTG AAAAGGCTGC CTCCTTTAAA CTGCAGCGTC AGAATCGTGT TGACAGCAAA
GAAACAGAGT GCTAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu60845D
Sequence Information ORF Nucleotide Sequence (Length: 1578bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product disintegrin and metalloproteinase domain-containing protein 17 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005334.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGCAAAAA GTTACCCAAA TGAAGAAAAG GATGCTTGGG ATGTGAAGAT GTTGCTAGAG 
CAATTTAGCT TTGATATAGC TGAGGAAGCA TCTAAAGTTT GCTTGGCACA CCTTTTCACA
TACCAAGATT TTGATATGGG AACTCTTGGA TTAGCTTATG TTGGCTCTCC CAGAGCAAAC
AGCCATGGAG GTGTTTGTCC AAAGGCTTAT TATAGCCCAG TTGGGAAGAA AAATATCTAT
TTGAATAGTG GTTTGACGAG CACAAAGAAT TATGGTAAAA CCATCCTTAC AAAGGAAGCT
GACCTGGTTA CAACTCATGA ATTGGGACAT AATTTTGGAG CAGAACATGA TCCGGATGGT
CTAGCAGAAT GTGCCCCGAA TGAGGACCAG GGAGGGAAAT ATGTCATGTA TCCCATAGCT
GTGAGTGGCG ATCACGAGAA CAATAAGATG TTTTCAAACT GCAGTAAACA ATCAATCTAT
AAGACCATTG AAAGTAAGGC CCAGGAGTGT TTTCAAGAAC GCAGCAATAA AGTTTGTGGG
AACTCGAGGG TGGATGAAGG AGAAGAGTGT GATCCTGGCA TCATGTATCT GAACAACGAC
ACCTGCTGCA ACAGCGACTG CACGTTGAAG GAAGGTGTCC AGTGCAGTGA CAGGAACAGT
CCTTGCTGTA AAAACTGTCA GTTTGAGACT GCCCAGAAGA AGTGCCAGGA GGCGATTAAT
GCTACTTGCA AAGGCGTGTC CTACTGCACA GGTAATAGCA GTGAGTGCCC GCCTCCAGGA
AATGCTGAAG ATGACACTGT TTGCTTGGAT CTTGGCAAGT GTAAGGATGG GAAATGCATC
CCTTTCTGCG AGAGGGAACA GCAGCTGGAG TCCTGTGCAT GTAATGAAAC TGACAACTCC
TGCAAGGTGT GCTGCAGGGA CCTTTCTGGC CGCTGTGTGC CCTATGTCGA TGCTGAACAA
AAGAACTTAT TTTTGAGGAA AGGAAAGCCC TGTACAGTAG GATTTTGTGA CATGAATGGC
AAATGTGAGA AACGAGTACA GGATGTAATT GAACGATTTT GGGATTTCAT TGACCAGCTG
AGCATCAATA CTTTTGGAAA GTTTTTAGCA GACAACATCG TTGGGTCTGT CCTGGTTTTC
TCCTTGATAT TTTGGATTCC TTTCAGCATT CTTGTCCATT GTGTGGATAA GAAATTGGAT
AAACAGTATG AATCTCTGTC TCTGTTTCAC CCCAGTAACG TCGAAATGCT GAGCAGCATG
GATTCTGCAT CGGTTCGCAT TATCAAACCC TTTCCTGCGC CCCAGACTCC AGGCCGCCTG
CAGCCTGCCC CTGTGATCCC TTCGGCGCCA GCAGCTCCAA AACTGGACCA CCAGAGAATG
GACACCATCC AGGAAGACCC CAGCACAGAC TCACATATGG ACGAGGATGG GTTTGAGAAG
GACCCCTTCC CAAATAGCAG CACAGCTGCC AAGTCATTTG AGGATCTCAC GGACCATCCG
GTCACCAGAA GTGAAAAGGC TGCCTCCTTT AAACTGCAGC GTCAGAATCG TGTTGACAGC
AAAGAAACAG AGTGCTAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011508679.1
CDS264..1841
Misc Feature(1)270..797(+)
Misc Feature(2)579..611(+)
Misc Feature(3)816..1046(+)
Misc Feature(4)1104..1292(+)
Misc Feature(5)1164..1271(+)
Misc Feature(6)1164..1175(+)
Translation
Position Chain Variation Link
13 13 a, t dbSNP:760052137
29 29 g, t dbSNP:557765135
34 34 a, c dbSNP:749977077
59 59 a, g dbSNP:12692384
101 101 c, g dbSNP:568989949
119 119 a, g dbSNP:761618194
143 143 c, t dbSNP:146893995
154 154 a, g dbSNP:778386605
158 158 c, t dbSNP:199651827
173 173 c, t dbSNP:200972600
176 176 a, c, g dbSNP:759217858
197 197 a, g dbSNP:751225336
208 208 a, c dbSNP:766171023
214 214 a, g dbSNP:375162225
221 221 c, t dbSNP:536745843
234 234 c, g dbSNP:773704430
239 239 a, g dbSNP:770336510
244 244 c, t dbSNP:748641439
246 246 a, g dbSNP:781586782
253 253 a, g dbSNP:570630045
269 269 a, g dbSNP:371791651
275 275 g, t dbSNP:746453821
294 294 c, g dbSNP:200988113
305 305 c, t dbSNP:201786766
306 306 a, g dbSNP:201123474
311 311 a, g dbSNP:757922213
313 313 -, t dbSNP:769140844
314 314 a, g dbSNP:367951580
343 343 c, t dbSNP:747554740
346 346 a, g dbSNP:779424893
352 352 c, t dbSNP:771618970
353 353 a, t dbSNP:745489345
358 358 a, c dbSNP:151089669
359 359 a, g dbSNP:756928057
360 360 a, g dbSNP:199788242
389 389 a, g dbSNP:573469509
398 398 c, t dbSNP:753521828
400 400 c, t dbSNP:777632615
407 407 a, t dbSNP:756005596
419 419 g, t dbSNP:752733411
432 432 a, c, t dbSNP:148983888
434 434 c, t dbSNP:750559090
440 440 a, g dbSNP:765505576
447 447 c, t dbSNP:201481056
468 468 g, t dbSNP:776793975
472 472 a, c dbSNP:752542509
484 484 a, t dbSNP:781232281
486 486 a, g dbSNP:199755899
492 492 a, g dbSNP:750470899
494 494 a, g dbSNP:765410644
502 502 a, g dbSNP:762120954
509 509 c, t dbSNP:754147588
520 520 c, t dbSNP:764334934
521 521 a, g dbSNP:761061102
527 527 a, g dbSNP:775742267
563 563 c, t dbSNP:201738148
569 569 c, g dbSNP:757397345
584 584 a, g dbSNP:753895855
604 604 c, t dbSNP:374447820
605 605 a, t dbSNP:756306371
616 616 c, t dbSNP:201789177
617 617 a, g dbSNP:767940322
619 619 a, g dbSNP:760026389
628 628 a, c dbSNP:774892528
630 630 c, g dbSNP:765839537
638 638 a, c dbSNP:762481146
641 641 a, g, t dbSNP:769427627
665 665 c, t dbSNP:747757837
678 678 a, g dbSNP:776562188
680 680 a, t dbSNP:768629467
683 683 c, t dbSNP:200399376
684 684 a, g dbSNP:780099488
686 686 a, g dbSNP:566565696
692 692 c, t dbSNP:749333487
693 693 a, g dbSNP:777722013
698 698 c, t dbSNP:756357298
699 699 a, g dbSNP:764909573
721 721 a, c dbSNP:748334117
738 738 a, g dbSNP:143293318
742 742 a, g dbSNP:201873770
757 757 a, g dbSNP:146433601
767 767 a, g dbSNP:746502094
779 779 a, g dbSNP:758941402
783 783 c, t dbSNP:750951308
784 784 a, g dbSNP:368697823
787 787 a, g dbSNP:761448887
794 794 a, c dbSNP:753420524
799 799 g, t dbSNP:376540653
808 808 c, t dbSNP:760407268
809 809 a, g dbSNP:144137945
814 814 c, t dbSNP:777478676
837 837 c, t dbSNP:55937573
847 847 c, t dbSNP:771817640
860 860 c, t dbSNP:759491821
861 861 a, g dbSNP:774191569
866 866 c, t dbSNP:770937007
876 876 a, g dbSNP:201743292
877 877 a, g dbSNP:200446953
879 879 a, g dbSNP:372967979
880 880 a, g dbSNP:747301065
886 886 c, t dbSNP:780454096
887 887 a, g dbSNP:758739864
897 897 a, g dbSNP:554069834
945 945 c, t dbSNP:779339431
952 952 c, g dbSNP:372323794
963 963 -, aag dbSNP:760760055
968 968 c, t dbSNP:757833957
976 976 c, t dbSNP:749866093
977 977 a, g dbSNP:368127694
979 979 c, t dbSNP:777392691
982 982 a, g dbSNP:755721769
987 987 a, g dbSNP:144014465
997 997 c, g dbSNP:375981644
998 998 c, t dbSNP:754676488
1000 1000 c, t dbSNP:751249609
1007 1007 c, t dbSNP:147475420
1010 1010 c, t dbSNP:762867130
1015 1015 a, g dbSNP:542316178
1019 1019 g, t dbSNP:754585612
1020 1020 a, g dbSNP:751286148
1040 1040 a, g dbSNP:779804030
1050 1050 a, g dbSNP:549183501
1051 1051 a, g dbSNP:750249923
1061 1061 a, c, t dbSNP:56237316
1062 1062 g, t dbSNP:752714332
1089 1089 c, g dbSNP:767693528
1095 1095 a, g dbSNP:759653644
1096 1096 a, g dbSNP:774751396
1100 1100 c, t dbSNP:771307389
1106 1106 c, t dbSNP:763358232
1109 1109 c, t dbSNP:773721139
1110 1110 c, t dbSNP:770135526
1112 1112 c, t dbSNP:748758847
1113 1113 a, g dbSNP:780557617
1121 1121 a, g dbSNP:768262672
1155 1155 g, t dbSNP:769076594
1190 1190 c, t dbSNP:1048610
1191 1191 a, g dbSNP:779554063
1194 1194 c, t dbSNP:771706819
1195 1195 a, g, t dbSNP:140536552
1199 1199 c, t dbSNP:200055210
1200 1200 a, g dbSNP:749066091
1205 1205 c, t dbSNP:777714532
1212 1212 a, g dbSNP:756129840
1215 1215 g, t dbSNP:751618802
1226 1226 g, t dbSNP:530963981
1233 1233 c, t dbSNP:766635709
1252 1252 c, t dbSNP:758683558
1259 1259 a, g dbSNP:750726481
1260 1260 a, g dbSNP:371598292
1268 1268 c, t dbSNP:762200753
1274 1274 c, t dbSNP:776882539
1296 1296 c, t dbSNP:749050766
1297 1297 a, g dbSNP:777431052
1298 1298 a, g dbSNP:756119780
1301 1301 a, g dbSNP:761422866
1308 1308 a, g dbSNP:748159167
1316 1316 a, g dbSNP:112463036
1320 1320 c, t dbSNP:780262610
1326 1326 a, g dbSNP:758594009
1336 1336 a, g dbSNP:144657795
1340 1340 g, t dbSNP:765452935
1347 1347 a, t dbSNP:757433621
1351 1351 a, g dbSNP:140933944
1360 1360 c, g dbSNP:745903113
1361 1361 a, c, g, t dbSNP:369229801
1363 1363 a, g dbSNP:778078194
1369 1369 c, t dbSNP:756429621
1382 1382 c, t dbSNP:753079319
1383 1383 a, g dbSNP:61754177
1397 1397 a, g dbSNP:759960474
1405 1405 -, cct dbSNP:746768799
1437 1437 a, g dbSNP:751073259
1439 1439 c, t dbSNP:34355677
1444 1444 a, g dbSNP:375942302
1458 1458 c, t dbSNP:576343468
1463 1463 a, t dbSNP:765883520
1470 1470 a, t dbSNP:762334638
1473 1473 c, g dbSNP:749976457
1477 1477 c, t dbSNP:747163792
1479 1479 -, ct dbSNP:767985722
1481 1481 a, g dbSNP:764926423
1484 1484 c, t dbSNP:370157347
1485 1485 -, ct dbSNP:760143401
1485 1485 c, t dbSNP:776564382
1491 1491 c, t dbSNP:778028713
1493 1493 c, t dbSNP:768383645
1502 1502 a, c, t dbSNP:775368008
1505 1505 c, t dbSNP:767287511
1506 1506 a, g dbSNP:369159078
1514 1514 a, g dbSNP:773264235
1520 1520 c, t dbSNP:202041986
1534 1534 c, g dbSNP:144721235
1539 1539 c, t dbSNP:748342054
1540 1540 a, g dbSNP:147275585
1552 1552 a, c dbSNP:768949233
1554 1554 c, t dbSNP:372189309
1561 1561 c, t dbSNP:181463078
1562 1562 a, g, t dbSNP:746436310
1565 1565 c, g dbSNP:148093044
1571 1571 c, g, t dbSNP:374390369
1572 1572 c, g dbSNP:763595824
1577 1577 c, t dbSNP:755792086
1578 1578 c, t dbSNP:370064783
1579 1579 a, g dbSNP:538865826
1580 1580 c, t dbSNP:759443841
1584 1584 a, c dbSNP:765817788
1585 1585 a, c dbSNP:774314959
1586 1586 a, g dbSNP:766337117
1589 1589 c, t dbSNP:200268337
1590 1590 a, g dbSNP:761836949
1606 1606 c, t dbSNP:55796712
1607 1607 a, g, t dbSNP:376456430
1608 1608 a, g dbSNP:761025971
1609 1609 c, t dbSNP:79932015
1614 1614 a, g dbSNP:374624230
1617 1617 g, t dbSNP:772616091
1627 1627 a, t dbSNP:746264614
1628 1628 c, g dbSNP:370037406
1636 1636 a, g dbSNP:779511668
1642 1642 c, t dbSNP:771579137
1645 1645 a, c dbSNP:748739807
1646 1646 c, t dbSNP:371018540
1648 1648 c, g dbSNP:755704723
1659 1659 a, c, g dbSNP:780815372
1662 1662 c, g dbSNP:754730403
1666 1666 a, g dbSNP:751381392
1669 1669 -, ca dbSNP:768475497
1674 1674 -, tc dbSNP:746900439
1682 1682 c, g dbSNP:766251160
1685 1685 c, t dbSNP:376241260
1686 1686 a, g dbSNP:753859098
1692 1692 a, g dbSNP:764293730
1693 1693 a, g dbSNP:760787134
1702 1702 a, g dbSNP:775739935
1706 1706 a, c dbSNP:772443121
1708 1708 a, c dbSNP:759921163
1709 1709 -, c dbSNP:779871088
1712 1712 c, t dbSNP:201360504
1714 1714 a, c dbSNP:78855175
1722 1722 a, g dbSNP:771710782
1745 1745 g, t dbSNP:771556813
1746 1746 a, g dbSNP:749860291
1753 1753 c, t dbSNP:572250428
1754 1754 a, g dbSNP:149361049
1762 1762 c, t dbSNP:747645870
1764 1764 g, t dbSNP:780850654
1781 1781 -, ggctgcctcctt dbSNP:771854768
1782 1782 a, c, g, t dbSNP:761391373
1789 1789 c, t dbSNP:758318148
1801 1801 a, g dbSNP:750426388
1803 1803 c, t dbSNP:764050476
1804 1804 a, g dbSNP:760757602
1812 1812 c, t dbSNP:752730764
1813 1813 a, g, t dbSNP:754284338
1818 1818 c, g dbSNP:142315365
1820 1820 -, cagcaaaga dbSNP:777944947
1832 1832 -, a dbSNP:756372734
1834 1834 -, ag dbSNP:781314197
1849 1849 c, t dbSNP:766895883
1851 1851 c, t dbSNP:763335447
1866 1866 -, ctta dbSNP:752044573
1867 1867 a, c dbSNP:773726116
1872 1872 c, g dbSNP:371014633
1874 1874 a, c dbSNP:747638598
1898 1898 -, acaaa dbSNP:756905441
1900 1900 -, a dbSNP:397871862
1902 1902 -, atcaa dbSNP:374487688
1902 1902 -, a dbSNP:3833563
1906 1906 a, g dbSNP:547401766
1916 1916 a, g dbSNP:6705408
1972 1972 c, t dbSNP:779832226
2037 2037 a, g dbSNP:377317204
2068 2068 c, g dbSNP:551774656
2124 2124 g, t dbSNP:769827227
2131 2131 a, g dbSNP:1130094
2194 2194 -, t dbSNP:5829218
2194 2194 g, t dbSNP:551456834
2203 2203 -, t dbSNP:111759422
2203 2203 a, t dbSNP:531849283
2221 2221 a, g dbSNP:73149881
2230 2230 a, g dbSNP:549261463
2245 2245 c, t dbSNP:529615832
2266 2266 -, tagt dbSNP:77130641
2272 2272 -, g dbSNP:550667141
2273 2273 g, t dbSNP:189060518
2287 2287 -, tttttt dbSNP:775488489
2287 2287 -, ttttt dbSNP:749119807
2287 2287 -, tttt dbSNP:774018291
2289 2289 -, tttt dbSNP:548548539

Target ORF information:

RefSeq Version XM_011510377
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ADAM metallopeptidase domain 17 (ADAM17), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011510377

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGCAAAAA GTTACCCAAA TGAAGAAAAG GATGCTTGGG ATGTGAAGAT GTTGCTAGAG 
CAATTTAGCT TTGATATAGC TGAGGAAGCA TCTAAAGTTT GCTTGGCACA CCTTTTCACA
TACCAAGATT TTGATATGGG AACTCTTGGA TTAGCTTATG TTGGCTCTCC CAGAGCAAAC
AGCCATGGAG GTGTTTGTCC AAAGGCTTAT TATAGCCCAG TTGGGAAGAA AAATATCTAT
TTGAATAGTG GTTTGACGAG CACAAAGAAT TATGGTAAAA CCATCCTTAC AAAGGAAGCT
GACCTGGTTA CAACTCATGA ATTGGGACAT AATTTTGGAG CAGAACATGA TCCGGATGGT
CTAGCAGAAT GTGCCCCGAA TGAGGACCAG GGAGGGAAAT ATGTCATGTA TCCCATAGCT
GTGAGTGGCG ATCACGAGAA CAATAAGATG TTTTCAAACT GCAGTAAACA ATCAATCTAT
AAGACCATTG AAAGTAAGGC CCAGGAGTGT TTTCAAGAAC GCAGCAATAA AGTTTGTGGG
AACTCGAGGG TGGATGAAGG AGAAGAGTGT GATCCTGGCA TCATGTATCT GAACAACGAC
ACCTGCTGCA ACAGCGACTG CACGTTGAAG GAAGGTGTCC AGTGCAGTGA CAGGAACAGT
CCTTGCTGTA AAAACTGTCA GTTTGAGACT GCCCAGAAGA AGTGCCAGGA GGCGATTAAT
GCTACTTGCA AAGGCGTGTC CTACTGCACA GGTAATAGCA GTGAGTGCCC GCCTCCAGGA
AATGCTGAAG ATGACACTGT TTGCTTGGAT CTTGGCAAGT GTAAGGATGG GAAATGCATC
CCTTTCTGCG AGAGGGAACA GCAGCTGGAG TCCTGTGCAT GTAATGAAAC TGACAACTCC
TGCAAGGTGT GCTGCAGGGA CCTTTCTGGC CGCTGTGTGC CCTATGTCGA TGCTGAACAA
AAGAACTTAT TTTTGAGGAA AGGAAAGCCC TGTACAGTAG GATTTTGTGA CATGAATGGC
AAATGTGAGA AACGAGTACA GGATGTAATT GAACGATTTT GGGATTTCAT TGACCAGCTG
AGCATCAATA CTTTTGGAAA GTTTTTAGCA GACAACATCG TTGGGTCTGT CCTGGTTTTC
TCCTTGATAT TTTGGATTCC TTTCAGCATT CTTGTCCATT GTGTGGATAA GAAATTGGAT
AAACAGTATG AATCTCTGTC TCTGTTTCAC CCCAGTAACG TCGAAATGCT GAGCAGCATG
GATTCTGCAT CGGTTCGCAT TATCAAACCC TTTCCTGCGC CCCAGACTCC AGGCCGCCTG
CAGCCTGCCC CTGTGATCCC TTCGGCGCCA GCAGCTCCAA AACTGGACCA CCAGAGAATG
GACACCATCC AGGAAGACCC CAGCACAGAC TCACATATGG ACGAGGATGG GTTTGAGAAG
GACCCCTTCC CAAATAGCAG CACAGCTGCC AAGTCATTTG AGGATCTCAC GGACCATCCG
GTCACCAGAA GTGAAAAGGC TGCCTCCTTT AAACTGCAGC GTCAGAATCG TGTTGACAGC
AAAGAAACAG AGTGCTAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu60845D
Sequence Information ORF Nucleotide Sequence (Length: 1578bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product disintegrin and metalloproteinase domain-containing protein 17 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005334.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGCAAAAA GTTACCCAAA TGAAGAAAAG GATGCTTGGG ATGTGAAGAT GTTGCTAGAG 
CAATTTAGCT TTGATATAGC TGAGGAAGCA TCTAAAGTTT GCTTGGCACA CCTTTTCACA
TACCAAGATT TTGATATGGG AACTCTTGGA TTAGCTTATG TTGGCTCTCC CAGAGCAAAC
AGCCATGGAG GTGTTTGTCC AAAGGCTTAT TATAGCCCAG TTGGGAAGAA AAATATCTAT
TTGAATAGTG GTTTGACGAG CACAAAGAAT TATGGTAAAA CCATCCTTAC AAAGGAAGCT
GACCTGGTTA CAACTCATGA ATTGGGACAT AATTTTGGAG CAGAACATGA TCCGGATGGT
CTAGCAGAAT GTGCCCCGAA TGAGGACCAG GGAGGGAAAT ATGTCATGTA TCCCATAGCT
GTGAGTGGCG ATCACGAGAA CAATAAGATG TTTTCAAACT GCAGTAAACA ATCAATCTAT
AAGACCATTG AAAGTAAGGC CCAGGAGTGT TTTCAAGAAC GCAGCAATAA AGTTTGTGGG
AACTCGAGGG TGGATGAAGG AGAAGAGTGT GATCCTGGCA TCATGTATCT GAACAACGAC
ACCTGCTGCA ACAGCGACTG CACGTTGAAG GAAGGTGTCC AGTGCAGTGA CAGGAACAGT
CCTTGCTGTA AAAACTGTCA GTTTGAGACT GCCCAGAAGA AGTGCCAGGA GGCGATTAAT
GCTACTTGCA AAGGCGTGTC CTACTGCACA GGTAATAGCA GTGAGTGCCC GCCTCCAGGA
AATGCTGAAG ATGACACTGT TTGCTTGGAT CTTGGCAAGT GTAAGGATGG GAAATGCATC
CCTTTCTGCG AGAGGGAACA GCAGCTGGAG TCCTGTGCAT GTAATGAAAC TGACAACTCC
TGCAAGGTGT GCTGCAGGGA CCTTTCTGGC CGCTGTGTGC CCTATGTCGA TGCTGAACAA
AAGAACTTAT TTTTGAGGAA AGGAAAGCCC TGTACAGTAG GATTTTGTGA CATGAATGGC
AAATGTGAGA AACGAGTACA GGATGTAATT GAACGATTTT GGGATTTCAT TGACCAGCTG
AGCATCAATA CTTTTGGAAA GTTTTTAGCA GACAACATCG TTGGGTCTGT CCTGGTTTTC
TCCTTGATAT TTTGGATTCC TTTCAGCATT CTTGTCCATT GTGTGGATAA GAAATTGGAT
AAACAGTATG AATCTCTGTC TCTGTTTCAC CCCAGTAACG TCGAAATGCT GAGCAGCATG
GATTCTGCAT CGGTTCGCAT TATCAAACCC TTTCCTGCGC CCCAGACTCC AGGCCGCCTG
CAGCCTGCCC CTGTGATCCC TTCGGCGCCA GCAGCTCCAA AACTGGACCA CCAGAGAATG
GACACCATCC AGGAAGACCC CAGCACAGAC TCACATATGG ACGAGGATGG GTTTGAGAAG
GACCCCTTCC CAAATAGCAG CACAGCTGCC AAGTCATTTG AGGATCTCAC GGACCATCCG
GTCACCAGAA GTGAAAAGGC TGCCTCCTTT AAACTGCAGC GTCAGAATCG TGTTGACAGC
AAAGAAACAG AGTGCTAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011508680.1
CDS270..1847
Misc Feature(1)276..803(+)
Misc Feature(2)585..617(+)
Misc Feature(3)822..1052(+)
Misc Feature(4)1110..1298(+)
Misc Feature(5)1170..1277(+)
Misc Feature(6)1170..1181(+)
Translation
Position Chain Variation Link
14 14 a, t dbSNP:760052137
30 30 g, t dbSNP:557765135
35 35 a, c dbSNP:749977077
60 60 a, g dbSNP:12692384
102 102 c, g dbSNP:568989949
120 120 a, g dbSNP:761618194
144 144 c, t dbSNP:146893995
155 155 a, g dbSNP:778386605
159 159 c, t dbSNP:199651827
174 174 c, t dbSNP:200972600
177 177 a, c, g dbSNP:759217858
198 198 a, g dbSNP:751225336
209 209 a, c dbSNP:766171023
211 211 c, t dbSNP:201461814
212 212 a, g dbSNP:759498105
213 213 c, t dbSNP:774514863
220 220 a, g dbSNP:375162225
227 227 c, t dbSNP:536745843
240 240 c, g dbSNP:773704430
245 245 a, g dbSNP:770336510
250 250 c, t dbSNP:748641439
252 252 a, g dbSNP:781586782
259 259 a, g dbSNP:570630045
275 275 a, g dbSNP:371791651
281 281 g, t dbSNP:746453821
300 300 c, g dbSNP:200988113
311 311 c, t dbSNP:201786766
312 312 a, g dbSNP:201123474
317 317 a, g dbSNP:757922213
319 319 -, t dbSNP:769140844
320 320 a, g dbSNP:367951580
349 349 c, t dbSNP:747554740
352 352 a, g dbSNP:779424893
358 358 c, t dbSNP:771618970
359 359 a, t dbSNP:745489345
364 364 a, c dbSNP:151089669
365 365 a, g dbSNP:756928057
366 366 a, g dbSNP:199788242
395 395 a, g dbSNP:573469509
404 404 c, t dbSNP:753521828
406 406 c, t dbSNP:777632615
413 413 a, t dbSNP:756005596
425 425 g, t dbSNP:752733411
438 438 a, c, t dbSNP:148983888
440 440 c, t dbSNP:750559090
446 446 a, g dbSNP:765505576
453 453 c, t dbSNP:201481056
474 474 g, t dbSNP:776793975
478 478 a, c dbSNP:752542509
490 490 a, t dbSNP:781232281
492 492 a, g dbSNP:199755899
498 498 a, g dbSNP:750470899
500 500 a, g dbSNP:765410644
508 508 a, g dbSNP:762120954
515 515 c, t dbSNP:754147588
526 526 c, t dbSNP:764334934
527 527 a, g dbSNP:761061102
533 533 a, g dbSNP:775742267
569 569 c, t dbSNP:201738148
575 575 c, g dbSNP:757397345
590 590 a, g dbSNP:753895855
610 610 c, t dbSNP:374447820
611 611 a, t dbSNP:756306371
622 622 c, t dbSNP:201789177
623 623 a, g dbSNP:767940322
625 625 a, g dbSNP:760026389
634 634 a, c dbSNP:774892528
636 636 c, g dbSNP:765839537
644 644 a, c dbSNP:762481146
647 647 a, g, t dbSNP:769427627
671 671 c, t dbSNP:747757837
684 684 a, g dbSNP:776562188
686 686 a, t dbSNP:768629467
689 689 c, t dbSNP:200399376
690 690 a, g dbSNP:780099488
692 692 a, g dbSNP:566565696
698 698 c, t dbSNP:749333487
699 699 a, g dbSNP:777722013
704 704 c, t dbSNP:756357298
705 705 a, g dbSNP:764909573
727 727 a, c dbSNP:748334117
744 744 a, g dbSNP:143293318
748 748 a, g dbSNP:201873770
763 763 a, g dbSNP:146433601
773 773 a, g dbSNP:746502094
785 785 a, g dbSNP:758941402
789 789 c, t dbSNP:750951308
790 790 a, g dbSNP:368697823
793 793 a, g dbSNP:761448887
800 800 a, c dbSNP:753420524
805 805 g, t dbSNP:376540653
814 814 c, t dbSNP:760407268
815 815 a, g dbSNP:144137945
820 820 c, t dbSNP:777478676
843 843 c, t dbSNP:55937573
853 853 c, t dbSNP:771817640
866 866 c, t dbSNP:759491821
867 867 a, g dbSNP:774191569
872 872 c, t dbSNP:770937007
882 882 a, g dbSNP:201743292
883 883 a, g dbSNP:200446953
885 885 a, g dbSNP:372967979
886 886 a, g dbSNP:747301065
892 892 c, t dbSNP:780454096
893 893 a, g dbSNP:758739864
903 903 a, g dbSNP:554069834
951 951 c, t dbSNP:779339431
958 958 c, g dbSNP:372323794
969 969 -, aag dbSNP:760760055
974 974 c, t dbSNP:757833957
982 982 c, t dbSNP:749866093
983 983 a, g dbSNP:368127694
985 985 c, t dbSNP:777392691
988 988 a, g dbSNP:755721769
993 993 a, g dbSNP:144014465
1003 1003 c, g dbSNP:375981644
1004 1004 c, t dbSNP:754676488
1006 1006 c, t dbSNP:751249609
1013 1013 c, t dbSNP:147475420
1016 1016 c, t dbSNP:762867130
1021 1021 a, g dbSNP:542316178
1025 1025 g, t dbSNP:754585612
1026 1026 a, g dbSNP:751286148
1046 1046 a, g dbSNP:779804030
1056 1056 a, g dbSNP:549183501
1057 1057 a, g dbSNP:750249923
1067 1067 a, c, t dbSNP:56237316
1068 1068 g, t dbSNP:752714332
1095 1095 c, g dbSNP:767693528
1101 1101 a, g dbSNP:759653644
1102 1102 a, g dbSNP:774751396
1106 1106 c, t dbSNP:771307389
1112 1112 c, t dbSNP:763358232
1115 1115 c, t dbSNP:773721139
1116 1116 c, t dbSNP:770135526
1118 1118 c, t dbSNP:748758847
1119 1119 a, g dbSNP:780557617
1127 1127 a, g dbSNP:768262672
1161 1161 g, t dbSNP:769076594
1196 1196 c, t dbSNP:1048610
1197 1197 a, g dbSNP:779554063
1200 1200 c, t dbSNP:771706819
1201 1201 a, g, t dbSNP:140536552
1205 1205 c, t dbSNP:200055210
1206 1206 a, g dbSNP:749066091
1211 1211 c, t dbSNP:777714532
1218 1218 a, g dbSNP:756129840
1221 1221 g, t dbSNP:751618802
1232 1232 g, t dbSNP:530963981
1239 1239 c, t dbSNP:766635709
1258 1258 c, t dbSNP:758683558
1265 1265 a, g dbSNP:750726481
1266 1266 a, g dbSNP:371598292
1274 1274 c, t dbSNP:762200753
1280 1280 c, t dbSNP:776882539
1302 1302 c, t dbSNP:749050766
1303 1303 a, g dbSNP:777431052
1304 1304 a, g dbSNP:756119780
1307 1307 a, g dbSNP:761422866
1314 1314 a, g dbSNP:748159167
1322 1322 a, g dbSNP:112463036
1326 1326 c, t dbSNP:780262610
1332 1332 a, g dbSNP:758594009
1342 1342 a, g dbSNP:144657795
1346 1346 g, t dbSNP:765452935
1353 1353 a, t dbSNP:757433621
1357 1357 a, g dbSNP:140933944
1366 1366 c, g dbSNP:745903113
1367 1367 a, c, g, t dbSNP:369229801
1369 1369 a, g dbSNP:778078194
1375 1375 c, t dbSNP:756429621
1388 1388 c, t dbSNP:753079319
1389 1389 a, g dbSNP:61754177
1403 1403 a, g dbSNP:759960474
1411 1411 -, cct dbSNP:746768799
1443 1443 a, g dbSNP:751073259
1445 1445 c, t dbSNP:34355677
1450 1450 a, g dbSNP:375942302
1464 1464 c, t dbSNP:576343468
1469 1469 a, t dbSNP:765883520
1476 1476 a, t dbSNP:762334638
1479 1479 c, g dbSNP:749976457
1483 1483 c, t dbSNP:747163792
1485 1485 -, ct dbSNP:767985722
1487 1487 a, g dbSNP:764926423
1490 1490 c, t dbSNP:370157347
1491 1491 -, ct dbSNP:760143401
1491 1491 c, t dbSNP:776564382
1497 1497 c, t dbSNP:778028713
1499 1499 c, t dbSNP:768383645
1508 1508 a, c, t dbSNP:775368008
1511 1511 c, t dbSNP:767287511
1512 1512 a, g dbSNP:369159078
1520 1520 a, g dbSNP:773264235
1526 1526 c, t dbSNP:202041986
1540 1540 c, g dbSNP:144721235
1545 1545 c, t dbSNP:748342054
1546 1546 a, g dbSNP:147275585
1558 1558 a, c dbSNP:768949233
1560 1560 c, t dbSNP:372189309
1567 1567 c, t dbSNP:181463078
1568 1568 a, g, t dbSNP:746436310
1571 1571 c, g dbSNP:148093044
1577 1577 c, g, t dbSNP:374390369
1578 1578 c, g dbSNP:763595824
1583 1583 c, t dbSNP:755792086
1584 1584 c, t dbSNP:370064783
1585 1585 a, g dbSNP:538865826
1586 1586 c, t dbSNP:759443841
1590 1590 a, c dbSNP:765817788
1591 1591 a, c dbSNP:774314959
1592 1592 a, g dbSNP:766337117
1595 1595 c, t dbSNP:200268337
1596 1596 a, g dbSNP:761836949
1612 1612 c, t dbSNP:55796712
1613 1613 a, g, t dbSNP:376456430
1614 1614 a, g dbSNP:761025971
1615 1615 c, t dbSNP:79932015
1620 1620 a, g dbSNP:374624230
1623 1623 g, t dbSNP:772616091
1633 1633 a, t dbSNP:746264614
1634 1634 c, g dbSNP:370037406
1642 1642 a, g dbSNP:779511668
1648 1648 c, t dbSNP:771579137
1651 1651 a, c dbSNP:748739807
1652 1652 c, t dbSNP:371018540
1654 1654 c, g dbSNP:755704723
1665 1665 a, c, g dbSNP:780815372
1668 1668 c, g dbSNP:754730403
1672 1672 a, g dbSNP:751381392
1675 1675 -, ca dbSNP:768475497
1680 1680 -, tc dbSNP:746900439
1688 1688 c, g dbSNP:766251160
1691 1691 c, t dbSNP:376241260
1692 1692 a, g dbSNP:753859098
1698 1698 a, g dbSNP:764293730
1699 1699 a, g dbSNP:760787134
1708 1708 a, g dbSNP:775739935
1712 1712 a, c dbSNP:772443121
1714 1714 a, c dbSNP:759921163
1715 1715 -, c dbSNP:779871088
1718 1718 c, t dbSNP:201360504
1720 1720 a, c dbSNP:78855175
1728 1728 a, g dbSNP:771710782
1751 1751 g, t dbSNP:771556813
1752 1752 a, g dbSNP:749860291
1759 1759 c, t dbSNP:572250428
1760 1760 a, g dbSNP:149361049
1768 1768 c, t dbSNP:747645870
1770 1770 g, t dbSNP:780850654
1787 1787 -, ggctgcctcctt dbSNP:771854768
1788 1788 a, c, g, t dbSNP:761391373
1795 1795 c, t dbSNP:758318148
1807 1807 a, g dbSNP:750426388
1809 1809 c, t dbSNP:764050476
1810 1810 a, g dbSNP:760757602
1818 1818 c, t dbSNP:752730764
1819 1819 a, g, t dbSNP:754284338
1824 1824 c, g dbSNP:142315365
1826 1826 -, cagcaaaga dbSNP:777944947
1838 1838 -, a dbSNP:756372734
1840 1840 -, ag dbSNP:781314197
1855 1855 c, t dbSNP:766895883
1857 1857 c, t dbSNP:763335447
1872 1872 -, ctta dbSNP:752044573
1873 1873 a, c dbSNP:773726116
1878 1878 c, g dbSNP:371014633
1880 1880 a, c dbSNP:747638598
1904 1904 -, acaaa dbSNP:756905441
1906 1906 -, a dbSNP:397871862
1908 1908 -, atcaa dbSNP:374487688
1908 1908 -, a dbSNP:3833563
1912 1912 a, g dbSNP:547401766
1922 1922 a, g dbSNP:6705408
1978 1978 c, t dbSNP:779832226
2043 2043 a, g dbSNP:377317204
2074 2074 c, g dbSNP:551774656
2130 2130 g, t dbSNP:769827227
2137 2137 a, g dbSNP:1130094
2200 2200 -, t dbSNP:5829218
2200 2200 g, t dbSNP:551456834
2209 2209 -, t dbSNP:111759422
2209 2209 a, t dbSNP:531849283
2227 2227 a, g dbSNP:73149881
2236 2236 a, g dbSNP:549261463
2251 2251 c, t dbSNP:529615832
2272 2272 -, tagt dbSNP:77130641
2278 2278 -, g dbSNP:550667141
2279 2279 g, t dbSNP:189060518
2293 2293 -, tttttt dbSNP:775488489
2293 2293 -, ttttt dbSNP:749119807
2293 2293 -, tttt dbSNP:774018291
2295 2295 -, tttt dbSNP:548548539

Target ORF information:

RefSeq Version XM_011510378
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens ADAM metallopeptidase domain 17 (ADAM17), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011510378

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGCAAAAA GTTACCCAAA TGAAGAAAAG GATGCTTGGG ATGTGAAGAT GTTGCTAGAG 
CAATTTAGCT TTGATATAGC TGAGGAAGCA TCTAAAGTTT GCTTGGCACA CCTTTTCACA
TACCAAGATT TTGATATGGG AACTCTTGGA TTAGCTTATG TTGGCTCTCC CAGAGCAAAC
AGCCATGGAG GTGTTTGTCC AAAGGCTTAT TATAGCCCAG TTGGGAAGAA AAATATCTAT
TTGAATAGTG GTTTGACGAG CACAAAGAAT TATGGTAAAA CCATCCTTAC AAAGGAAGCT
GACCTGGTTA CAACTCATGA ATTGGGACAT AATTTTGGAG CAGAACATGA TCCGGATGGT
CTAGCAGAAT GTGCCCCGAA TGAGGACCAG GGAGGGAAAT ATGTCATGTA TCCCATAGCT
GTGAGTGGCG ATCACGAGAA CAATAAGATG TTTTCAAACT GCAGTAAACA ATCAATCTAT
AAGACCATTG AAAGTAAGGC CCAGGAGTGT TTTCAAGAAC GCAGCAATAA AGTTTGTGGG
AACTCGAGGG TGGATGAAGG AGAAGAGTGT GATCCTGGCA TCATGTATCT GAACAACGAC
ACCTGCTGCA ACAGCGACTG CACGTTGAAG GAAGGTGTCC AGTGCAGTGA CAGGAACAGT
CCTTGCTGTA AAAACTGTCA GTTTGAGACT GCCCAGAAGA AGTGCCAGGA GGCGATTAAT
GCTACTTGCA AAGGCGTGTC CTACTGCACA GGTAATAGCA GTGAGTGCCC GCCTCCAGGA
AATGCTGAAG ATGACACTGT TTGCTTGGAT CTTGGCAAGT GTAAGGATGG GAAATGCATC
CCTTTCTGCG AGAGGGAACA GCAGCTGGAG TCCTGTGCAT GTAATGAAAC TGACAACTCC
TGCAAGGTGT GCTGCAGGGA CCTTTCTGGC CGCTGTGTGC CCTATGTCGA TGCTGAACAA
AAGAACTTAT TTTTGAGGAA AGGAAAGCCC TGTACAGTAG GATTTTGTGA CATGAATGGC
AAATGTGAGA AACGAGTACA GGATGTAATT GAACGATTTT GGGATTTCAT TGACCAGCTG
AGCATCAATA CTTTTGGAAA GTTTTTAGCA GACAACATCG TTGGGTCTGT CCTGGTTTTC
TCCTTGATAT TTTGGATTCC TTTCAGCATT CTTGTCCATT GTGTGGATAA GAAATTGGAT
AAACAGTATG AATCTCTGTC TCTGTTTCAC CCCAGTAACG TCGAAATGCT GAGCAGCATG
GATTCTGCAT CGGTTCGCAT TATCAAACCC TTTCCTGCGC CCCAGACTCC AGGCCGCCTG
CAGCCTGCCC CTGTGATCCC TTCGGCGCCA GCAGCTCCAA AACTGGACCA CCAGAGAATG
GACACCATCC AGGAAGACCC CAGCACAGAC TCACATATGG ACGAGGATGG GTTTGAGAAG
GACCCCTTCC CAAATAGCAG CACAGCTGCC AAGTCATTTG AGGATCTCAC GGACCATCCG
GTCACCAGAA GTGAAAAGGC TGCCTCCTTT AAACTGCAGC GTCAGAATCG TGTTGACAGC
AAAGAAACAG AGTGCTAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu21940D
Sequence Information ORF Nucleotide Sequence (Length: 2475bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product disintegrin and metalloproteinase domain-containing protein 17 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN258241.1, U86755.1, BX339672.2, U92649.1, U69611.1, BM725368.1 and BQ186514.1. This sequence is a reference standard in the RefSeqGene project. On Aug 24, 2005 this sequence version replaced gi:11497003. Summary: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GQ402545.1, U92649.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGAGGCAGT CTCTCCTATT CCTGACCAGC GTGGTTCCTT TCGTGCTGGC GCCGCGACCT 
CCGGATGACC CGGGCTTCGG CCCCCACCAG AGACTCGAGA AGCTTGATTC TTTGCTCTCA
GACTACGATA TTCTCTCTTT ATCTAATATC CAGCAGCATT CGGTAAGAAA AAGAGATCTA
CAGACTTCAA CACATGTAGA AACACTACTA ACTTTTTCAG CTTTGAAAAG GCATTTTAAA
TTATACCTGA CATCAAGTAC TGAACGTTTT TCACAAAATT TCAAGGTCGT GGTGGTGGAT
GGTAAAAACG AAAGCGAGTA CACTGTAAAA TGGCAGGACT TCTTCACTGG ACACGTGGTT
GGTGAGCCTG ACTCTAGGGT TCTAGCCCAC ATAAGAGATG ATGATGTTAT AATCAGAATC
AACACAGATG GGGCCGAATA TAACATAGAG CCACTTTGGA GATTTGTTAA TGATACCAAA
GACAAAAGAA TGTTAGTTTA TAAATCTGAA GATATCAAGA ATGTTTCACG TTTGCAGTCT
CCAAAAGTGT GTGGTTATTT AAAAGTGGAT AATGAAGAGT TGCTCCCAAA AGGGTTAGTA
GACAGAGAAC CACCTGAAGA GCTTGTTCAT CGAGTGAAAA GAAGAGCTGA CCCAGATCCC
ATGAAGAACA CGTGTAAATT ATTGGTGGTA GCAGATCATC GCTTCTACAG ATACATGGGC
AGAGGGGAAG AGAGTACAAC TACAAATTAC TTAATAGAGC TAATTGACAG AGTTGATGAC
ATCTATCGGA ACACTTCATG GGATAATGCA GGTTTTAAAG GCTATGGAAT ACAGATAGAG
CAGATTCGCA TTCTCAAGTC TCCACAAGAG GTAAAACCTG GTGAAAAGCA CTACAACATG
GCAAAAAGTT ACCCAAATGA AGAAAAGGAT GCTTGGGATG TGAAGATGTT GCTAGAGCAA
TTTAGCTTTG ATATAGCTGA GGAAGCATCT AAAGTTTGCT TGGCACACCT TTTCACATAC
CAAGATTTTG ATATGGGAAC TCTTGGATTA GCTTATGTTG GCTCTCCCAG AGCAAACAGC
CATGGAGGTG TTTGTCCAAA GGCTTATTAT AGCCCAGTTG GGAAGAAAAA TATCTATTTG
AATAGTGGTT TGACGAGCAC AAAGAATTAT GGTAAAACCA TCCTTACAAA GGAAGCTGAC
CTGGTTACAA CTCATGAATT GGGACATAAT TTTGGAGCAG AACATGATCC GGATGGTCTA
GCAGAATGTG CCCCGAATGA GGACCAGGGA GGGAAATATG TCATGTATCC CATAGCTGTG
AGTGGCGATC ACGAGAACAA TAAGATGTTT TCAAACTGCA GTAAACAATC AATCTATAAG
ACCATTGAAA GTAAGGCCCA GGAGTGTTTT CAAGAACGCA GCAATAAAGT TTGTGGGAAC
TCGAGGGTGG ATGAAGGAGA AGAGTGTGAT CCTGGCATCA TGTATCTGAA CAACGACACC
TGCTGCAACA GCGACTGCAC GTTGAAGGAA GGTGTCCAGT GCAGTGACAG GAACAGTCCT
TGCTGTAAAA ACTGTCAGTT TGAGACTGCC CAGAAGAAGT GCCAGGAGGC GATTAATGCT
ACTTGCAAAG GCGTGTCCTA CTGCACAGGT AATAGCAGTG AGTGCCCGCC TCCAGGAAAT
GCTGAAGATG ACACTGTTTG CTTGGATCTT GGCAAGTGTA AGGATGGGAA ATGCATCCCT
TTCTGCGAGA GGGAACAGCA GCTGGAGTCC TGTGCATGTA ATGAAACTGA CAACTCCTGC
AAGGTGTGCT GCAGGGACCT TTCTGGCCGC TGTGTGCCCT ATGTCGATGC TGAACAAAAG
AACTTATTTT TGAGGAAAGG AAAGCCCTGT ACAGTAGGAT TTTGTGACAT GAATGGCAAA
TGTGAGAAAC GAGTACAGGA TGTAATTGAA CGATTTTGGG ATTTCATTGA CCAGCTGAGC
ATCAATACTT TTGGAAAGTT TTTAGCAGAC AACATCGTTG GGTCTGTCCT GGTTTTCTCC
TTGATATTTT GGATTCCTTT CAGCATTCTT GTCCATTGTG TGGATAAGAA ATTGGATAAA
CAGTATGAAT CTCTGTCTCT GTTTCACCCC AGTAACGTCG AAATGCTGAG CAGCATGGAT
TCTGCATCGG TTCGCATTAT CAAACCCTTT CCTGCGCCCC AGACTCCAGG CCGCCTGCAG
CCTGCCCCTG TGATCCCTTC GGCGCCAGCA GCTCCAAAAC TGGACCACCA GAGAATGGAC
ACCATCCAGG AAGACCCCAG CACAGACTCA CATATGGACG AGGATGGGTT TGAGAAGGAC
CCCTTCCCAA ATAGCAGCAC AGCTGCCAAG TCATTTGAGG ATCTCACGGA CCATCCGGTC
ACCAGAAGTG AAAAGGCTGC CTCCTTTAAA CTGCAGCGTC AGAATCGTGT TGACAGCAAA
GAAACAGAGT GCTAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_003174.3
CDS184..2658
Misc Feature(1)82..84(+)
Misc Feature(2)307..684(+)
Misc Feature(3)727..750(+)
Misc Feature(4)850..1614(+)
Misc Feature(5)1318..1320(+)
Misc Feature(6)1327..1329(+)
Misc Feature(7)1396..1428(+)
Misc Feature(8)1633..1863(+)
Misc Feature(9)1894..1989(+)
Misc Feature(10)1921..2109(+)
Misc Feature(11)1981..2088(+)
Misc Feature(12)1981..1992(+)
Misc Feature(13)1990..2196(+)
Misc Feature(14)2197..2265(+)
Misc Feature(15)2197..2259(+)
Misc Feature(16)2266..2655(+)
Misc Feature(17)2386..2388(+)
Misc Feature(18)2554..2556(+)
Misc Feature(19)2554..2556(+)
Misc Feature(20)2554..2556(+)
Misc Feature(21)2638..2640(+)
Misc Feature(22)2638..2640(+)
Exon (1)1..280
Gene:ADAM17
Gene Synonym:
Exon (2)281..413
Gene:ADAM17
Gene Synonym:
Exon (3)414..544
Gene:ADAM17
Gene Synonym:
Exon (4)545..633
Gene:ADAM17
Gene Synonym:
Exon (5)634..802
Gene:ADAM17
Gene Synonym:
Exon (6)803..936
Gene:ADAM17
Gene Synonym:
Exon (7)937..1026
Gene:ADAM17
Gene Synonym:
Exon (8)1027..1140
Gene:ADAM17
Gene Synonym:
Exon (9)1141..1285
Gene:ADAM17
Gene Synonym:
Exon (10)1286..1374
Gene:ADAM17
Gene Synonym:
Exon (11)1375..1527
Gene:ADAM17
Gene Synonym:
Exon (12)1528..1727
Gene:ADAM17
Gene Synonym:
Exon (13)1728..1831
Gene:ADAM17
Gene Synonym:
Exon (14)1832..1966
Gene:ADAM17
Gene Synonym:
Exon (15)1967..2097
Gene:ADAM17
Gene Synonym:
Exon (16)2098..2176
Gene:ADAM17
Gene Synonym:
Exon (17)2177..2265
Gene:ADAM17
Gene Synonym:
Exon (18)2266..2316
Gene:ADAM17
Gene Synonym:
Exon (19)2317..3572
Gene:ADAM17
Gene Synonym:
Translation
Position Chain Variation Link
12 12 c, t dbSNP:12692386
22 22 a, g dbSNP:547394870
30 30 a, c dbSNP:55790676
32 32 g, t dbSNP:565141488
41 41 c, t dbSNP:189110504
72 72 a, c dbSNP:749950472
138 138 a, g dbSNP:576655902
143 143 a, g dbSNP:760272179
146 146 c, g dbSNP:200520450
148 148 g, t dbSNP:139691809
153 153 a, g dbSNP:368396944
165 165 c, t dbSNP:745531826
166 166 c, g, t dbSNP:201127286
167 167 a, g dbSNP:770914877
168 168 a, t dbSNP:757161796
170 170 a, g dbSNP:749165284
175 175 c, g, t dbSNP:554296810
177 177 c, t dbSNP:747070833
178 178 a, g dbSNP:780128597
188 188 c, g dbSNP:758557343
193 193 c, t dbSNP:201578288
201 201 a, g dbSNP:774461179
202 202 g, t dbSNP:765575403
210 210 c, g dbSNP:757690124
214 214 g, t dbSNP:754268931
222 222 c, t dbSNP:764622550
225 225 a, c dbSNP:578189296
226 226 c, g dbSNP:775086314
229 229 c, g dbSNP:768569416
230 230 -, t dbSNP:763323613
232 232 a, g dbSNP:759260210
235 235 c, t dbSNP:558316493
236 236 c, t dbSNP:144458353
242 242 c, g dbSNP:749144062
246 246 a, g dbSNP:772911575
247 247 g, t dbSNP:146033784
253 253 c, g dbSNP:748048199
254 254 c, t dbSNP:780040821
264 264 c, t dbSNP:138046330
269 269 a, g dbSNP:182108217
272 272 a, g dbSNP:775793715
277 277 c, g dbSNP:779147649
278 278 c, t dbSNP:757451849
293 293 a, c dbSNP:771131237
310 310 a, g dbSNP:749480182
313 313 a, g dbSNP:778198013
324 324 a, g dbSNP:756496579
326 326 c, g, t dbSNP:781773909
331 331 a, g dbSNP:61754178
341 341 a, g dbSNP:751060367
344 344 c, t dbSNP:765820179
345 345 a, g dbSNP:762599369
346 346 g, t dbSNP:752745494
349 349 a, g dbSNP:750108089
358 358 a, g dbSNP:765061007
361 361 a, c dbSNP:112594248
363 363 a, g dbSNP:776442513
366 366 a, g dbSNP:768531855
375 375 a, g dbSNP:760623120
377 377 a, g dbSNP:774425802
380 380 c, t dbSNP:770985771
387 387 a, g dbSNP:567931151
388 388 c, g dbSNP:777902756
390 390 a, g dbSNP:141846970
395 395 a, c dbSNP:748480175
402 402 a, c dbSNP:113429723
405 405 c, t dbSNP:781703932
415 415 c, g dbSNP:77759270
422 422 a, g dbSNP:775350065
423 423 a, g dbSNP:766463243
432 432 c, g dbSNP:762979463
449 449 a, g dbSNP:773370348
450 450 c, t dbSNP:770087502
469 469 a, g dbSNP:748390461
472 472 g, t dbSNP:377513734
473 473 c, t dbSNP:568438975
477 477 g, t dbSNP:149899785
482 482 a, t dbSNP:747536329
484 484 a, g dbSNP:780467691
492 492 c, t dbSNP:761183996
493 493 a, g dbSNP:745314265
498 498 c, t dbSNP:552799044
499 499 a, g dbSNP:756840167
500 500 a, g dbSNP:753558493
504 504 c, t dbSNP:576734742
505 505 a, g dbSNP:755955503
506 506 a, c dbSNP:752645542
518 518 a, g dbSNP:767315925
525 525 c, g dbSNP:759536569
534 534 a, g dbSNP:773103941
537 537 c, t dbSNP:373534760
538 538 -, g dbSNP:769696107
541 541 a, g, t dbSNP:370085865
545 545 g, t dbSNP:760965778
550 550 c, t dbSNP:775650699
556 556 a, t dbSNP:772557660
574 574 a, g dbSNP:759951895
579 579 a, g dbSNP:774890561
580 580 a, g dbSNP:199524645
584 584 a, c dbSNP:770400357
591 591 c, t dbSNP:538546425
616 616 a, g dbSNP:777284700
618 618 c, g, t dbSNP:143124556
621 621 a, g dbSNP:780843854
625 625 a, c dbSNP:754860343
645 645 a, g dbSNP:549352854
657 657 c, t dbSNP:562528833
667 667 a, g dbSNP:34431503
672 672 a, c dbSNP:752833089
675 675 a, g dbSNP:374243206
680 680 c, t dbSNP:755211714
688 688 g, t dbSNP:560843913
698 698 c, t dbSNP:778942509
713 713 a, g dbSNP:763442293
719 719 a, g dbSNP:139401611
721 721 c, t dbSNP:764631120
745 745 a, g dbSNP:761424749
750 750 a, g dbSNP:776034976
751 751 g, t dbSNP:768297455
760 760 a, g dbSNP:746664897
776 776 c, g dbSNP:775422299
786 786 -, caga dbSNP:387906866
787 787 a, g, t dbSNP:2230818
814 814 c, t dbSNP:774040338
819 819 a, g dbSNP:770840827
827 827 g, t dbSNP:142946965
835 835 a, c dbSNP:373192089
837 837 a, g dbSNP:755105372
845 845 g, t dbSNP:200778881
849 849 g, t dbSNP:780293025
851 851 a, g dbSNP:758650568
852 852 c, t dbSNP:750750076
854 854 c, t dbSNP:548923032
855 855 a, g, t dbSNP:757777916
879 879 c, t dbSNP:768704961
880 880 c, t dbSNP:754362767
885 885 c, t dbSNP:763677117
899 899 c, t dbSNP:760305057
921 921 -, aac dbSNP:777536319
926 926 c, t dbSNP:752288692
928 928 a, g dbSNP:369378891
960 960 c, t dbSNP:146893995
971 971 a, g dbSNP:778386605
975 975 c, t dbSNP:199651827
990 990 c, t dbSNP:200972600
993 993 a, c, g dbSNP:759217858
1014 1014 a, g dbSNP:751225336
1025 1025 a, c dbSNP:766171023
1031 1031 a, g dbSNP:375162225
1038 1038 c, t dbSNP:536745843
1051 1051 c, g dbSNP:773704430
1056 1056 a, g dbSNP:770336510
1061 1061 c, t dbSNP:748641439
1063 1063 a, g dbSNP:781586782
1070 1070 a, g dbSNP:570630045
1086 1086 a, g dbSNP:371791651
1092 1092 g, t dbSNP:746453821
1111 1111 c, g dbSNP:200988113
1122 1122 c, t dbSNP:201786766
1123 1123 a, g dbSNP:201123474
1128 1128 a, g dbSNP:757922213
1130 1130 -, t dbSNP:769140844
1131 1131 a, g dbSNP:367951580
1160 1160 c, t dbSNP:747554740
1163 1163 a, g dbSNP:779424893
1169 1169 c, t dbSNP:771618970
1170 1170 a, t dbSNP:745489345
1175 1175 a, c dbSNP:151089669
1176 1176 a, g dbSNP:756928057
1177 1177 a, g dbSNP:199788242
1206 1206 a, g dbSNP:573469509
1215 1215 c, t dbSNP:753521828
1217 1217 c, t dbSNP:777632615
1224 1224 a, t dbSNP:756005596
1236 1236 g, t dbSNP:752733411
1249 1249 a, c, t dbSNP:148983888
1251 1251 c, t dbSNP:750559090
1257 1257 a, g dbSNP:765505576
1264 1264 c, t dbSNP:201481056
1285 1285 g, t dbSNP:776793975
1289 1289 a, c dbSNP:752542509
1301 1301 a, t dbSNP:781232281
1303 1303 a, g dbSNP:199755899
1309 1309 a, g dbSNP:750470899
1311 1311 a, g dbSNP:765410644
1319 1319 a, g dbSNP:762120954
1326 1326 c, t dbSNP:754147588
1337 1337 c, t dbSNP:764334934
1338 1338 a, g dbSNP:761061102
1344 1344 a, g dbSNP:775742267
1380 1380 c, t dbSNP:201738148
1386 1386 c, g dbSNP:757397345
1401 1401 a, g dbSNP:753895855
1421 1421 c, t dbSNP:374447820
1422 1422 a, t dbSNP:756306371
1433 1433 c, t dbSNP:201789177
1434 1434 a, g dbSNP:767940322
1436 1436 a, g dbSNP:760026389
1445 1445 a, c dbSNP:774892528
1447 1447 c, g dbSNP:765839537
1455 1455 a, c dbSNP:762481146
1458 1458 a, g, t dbSNP:769427627
1482 1482 c, t dbSNP:747757837
1495 1495 a, g dbSNP:776562188
1497 1497 a, t dbSNP:768629467
1500 1500 c, t dbSNP:200399376
1501 1501 a, g dbSNP:780099488
1503 1503 a, g dbSNP:566565696
1509 1509 c, t dbSNP:749333487
1510 1510 a, g dbSNP:777722013
1515 1515 c, t dbSNP:756357298
1516 1516 a, g dbSNP:764909573
1538 1538 a, c dbSNP:748334117
1555 1555 a, g dbSNP:143293318
1559 1559 a, g dbSNP:201873770
1574 1574 a, g dbSNP:146433601
1584 1584 a, g dbSNP:746502094
1596 1596 a, g dbSNP:758941402
1600 1600 c, t dbSNP:750951308
1601 1601 a, g dbSNP:368697823
1604 1604 a, g dbSNP:761448887
1611 1611 a, c dbSNP:753420524
1616 1616 g, t dbSNP:376540653
1625 1625 c, t dbSNP:760407268
1626 1626 a, g dbSNP:144137945
1631 1631 c, t dbSNP:777478676
1654 1654 c, t dbSNP:55937573
1664 1664 c, t dbSNP:771817640
1677 1677 c, t dbSNP:759491821
1678 1678 a, g dbSNP:774191569
1683 1683 c, t dbSNP:770937007
1693 1693 a, g dbSNP:201743292
1694 1694 a, g dbSNP:200446953
1696 1696 a, g dbSNP:372967979
1697 1697 a, g dbSNP:747301065
1703 1703 c, t dbSNP:780454096
1704 1704 a, g dbSNP:758739864
1714 1714 a, g dbSNP:554069834
1762 1762 c, t dbSNP:779339431
1769 1769 c, g dbSNP:372323794
1780 1780 -, aag dbSNP:760760055
1785 1785 c, t dbSNP:757833957
1793 1793 c, t dbSNP:749866093
1794 1794 a, g dbSNP:368127694
1796 1796 c, t dbSNP:777392691
1799 1799 a, g dbSNP:755721769
1804 1804 a, g dbSNP:144014465
1814 1814 c, g dbSNP:375981644
1815 1815 c, t dbSNP:754676488
1817 1817 c, t dbSNP:751249609
1824 1824 c, t dbSNP:147475420
1827 1827 c, t dbSNP:762867130
1832 1832 a, g dbSNP:542316178
1836 1836 g, t dbSNP:754585612
1837 1837 a, g dbSNP:751286148
1857 1857 a, g dbSNP:779804030
1867 1867 a, g dbSNP:549183501
1868 1868 a, g dbSNP:750249923
1878 1878 a, c, t dbSNP:56237316
1879 1879 g, t dbSNP:752714332
1906 1906 c, g dbSNP:767693528
1912 1912 a, g dbSNP:759653644
1913 1913 a, g dbSNP:774751396
1917 1917 c, t dbSNP:771307389
1923 1923 c, t dbSNP:763358232
1926 1926 c, t dbSNP:773721139
1927 1927 c, t dbSNP:770135526
1929 1929 c, t dbSNP:748758847
1930 1930 a, g dbSNP:780557617
1938 1938 a, g dbSNP:768262672
1972 1972 g, t dbSNP:769076594
2007 2007 c, t dbSNP:1048610
2008 2008 a, g dbSNP:779554063
2011 2011 c, t dbSNP:771706819
2012 2012 a, g, t dbSNP:140536552
2016 2016 c, t dbSNP:200055210
2017 2017 a, g dbSNP:749066091
2022 2022 c, t dbSNP:777714532
2029 2029 a, g dbSNP:756129840
2032 2032 g, t dbSNP:751618802
2043 2043 g, t dbSNP:530963981
2050 2050 c, t dbSNP:766635709
2069 2069 c, t dbSNP:758683558
2076 2076 a, g dbSNP:750726481
2077 2077 a, g dbSNP:371598292
2085 2085 c, t dbSNP:762200753
2091 2091 c, t dbSNP:776882539
2113 2113 c, t dbSNP:749050766
2114 2114 a, g dbSNP:777431052
2115 2115 a, g dbSNP:756119780
2118 2118 a, g dbSNP:761422866
2125 2125 a, g dbSNP:748159167
2133 2133 a, g dbSNP:112463036
2137 2137 c, t dbSNP:780262610
2143 2143 a, g dbSNP:758594009
2153 2153 a, g dbSNP:144657795
2157 2157 g, t dbSNP:765452935
2164 2164 a, t dbSNP:757433621
2168 2168 a, g dbSNP:140933944
2177 2177 c, g dbSNP:745903113
2178 2178 a, c, g, t dbSNP:369229801
2180 2180 a, g dbSNP:778078194
2186 2186 c, t dbSNP:756429621
2199 2199 c, t dbSNP:753079319
2200 2200 a, g dbSNP:61754177
2214 2214 a, g dbSNP:759960474
2222 2222 -, cct dbSNP:746768799
2254 2254 a, g dbSNP:751073259
2256 2256 c, t dbSNP:34355677
2261 2261 a, g dbSNP:375942302
2275 2275 c, t dbSNP:576343468
2280 2280 a, t dbSNP:765883520
2287 2287 a, t dbSNP:762334638
2290 2290 c, g dbSNP:749976457
2294 2294 c, t dbSNP:747163792
2296 2296 -, ct dbSNP:767985722
2298 2298 a, g dbSNP:764926423
2301 2301 c, t dbSNP:370157347
2302 2302 -, ct dbSNP:760143401
2302 2302 c, t dbSNP:776564382
2308 2308 c, t dbSNP:778028713
2310 2310 c, t dbSNP:768383645
2319 2319 a, c, t dbSNP:775368008
2322 2322 c, t dbSNP:767287511
2323 2323 a, g dbSNP:369159078
2331 2331 a, g dbSNP:773264235
2337 2337 c, t dbSNP:202041986
2351 2351 c, g dbSNP:144721235
2356 2356 c, t dbSNP:748342054
2357 2357 a, g dbSNP:147275585
2369 2369 a, c dbSNP:768949233
2371 2371 c, t dbSNP:372189309
2378 2378 c, t dbSNP:181463078
2379 2379 a, g, t dbSNP:746436310
2382 2382 c, g dbSNP:148093044
2388 2388 c, g, t dbSNP:374390369
2389 2389 c, g dbSNP:763595824
2394 2394 c, t dbSNP:755792086
2395 2395 c, t dbSNP:370064783
2396 2396 a, g dbSNP:538865826
2397 2397 c, t dbSNP:759443841
2401 2401 a, c dbSNP:765817788
2402 2402 a, c dbSNP:774314959
2403 2403 a, g dbSNP:766337117
2406 2406 c, t dbSNP:200268337
2407 2407 a, g dbSNP:761836949
2423 2423 c, t dbSNP:55796712
2424 2424 a, g, t dbSNP:376456430
2425 2425 a, g dbSNP:761025971
2426 2426 c, t dbSNP:79932015
2431 2431 a, g dbSNP:374624230
2434 2434 g, t dbSNP:772616091
2444 2444 a, t dbSNP:746264614
2445 2445 c, g dbSNP:370037406
2453 2453 a, g dbSNP:779511668
2459 2459 c, t dbSNP:771579137
2462 2462 a, c dbSNP:748739807
2463 2463 c, t dbSNP:371018540
2465 2465 c, g dbSNP:755704723
2476 2476 a, c, g dbSNP:780815372
2479 2479 c, g dbSNP:754730403
2483 2483 a, g dbSNP:751381392
2486 2486 -, ca dbSNP:768475497
2491 2491 -, tc dbSNP:746900439
2499 2499 c, g dbSNP:766251160
2502 2502 c, t dbSNP:376241260
2503 2503 a, g dbSNP:753859098
2509 2509 a, g dbSNP:764293730
2510 2510 a, g dbSNP:760787134
2519 2519 a, g dbSNP:775739935
2523 2523 a, c dbSNP:772443121
2525 2525 a, c dbSNP:759921163
2526 2526 -, c dbSNP:779871088
2529 2529 c, t dbSNP:201360504
2531 2531 a, c dbSNP:78855175
2539 2539 a, g dbSNP:771710782
2562 2562 g, t dbSNP:771556813
2563 2563 a, g dbSNP:749860291
2570 2570 c, t dbSNP:572250428
2571 2571 a, g dbSNP:149361049
2579 2579 c, t dbSNP:747645870
2581 2581 g, t dbSNP:780850654
2598 2598 -, ggctgcctcctt dbSNP:771854768
2599 2599 a, c, g, t dbSNP:761391373
2606 2606 c, t dbSNP:758318148
2618 2618 a, g dbSNP:750426388
2620 2620 c, t dbSNP:764050476
2621 2621 a, g dbSNP:760757602
2629 2629 c, t dbSNP:752730764
2630 2630 a, g, t dbSNP:754284338
2635 2635 c, g dbSNP:142315365
2637 2637 -, cagcaaaga dbSNP:777944947
2649 2649 -, a dbSNP:756372734
2651 2651 -, ag dbSNP:781314197
2666 2666 c, t dbSNP:766895883
2668 2668 c, t dbSNP:763335447
2683 2683 -, ctta dbSNP:752044573
2684 2684 a, c dbSNP:773726116
2689 2689 c, g dbSNP:371014633
2691 2691 a, c dbSNP:747638598
2715 2715 -, acaaa dbSNP:756905441
2717 2717 -, a dbSNP:397871862
2719 2719 -, atcaa dbSNP:374487688
2719 2719 -, a dbSNP:3833563
2723 2723 a, g dbSNP:547401766
2733 2733 a, g dbSNP:6705408
2789 2789 c, t dbSNP:779832226
2854 2854 a, g dbSNP:377317204
2885 2885 c, g dbSNP:551774656
2941 2941 g, t dbSNP:769827227
2948 2948 a, g dbSNP:1130094
3011 3011 -, t dbSNP:5829218
3011 3011 g, t dbSNP:551456834
3020 3020 -, t dbSNP:111759422
3020 3020 a, t dbSNP:531849283
3038 3038 a, g dbSNP:73149881
3047 3047 a, g dbSNP:549261463
3062 3062 c, t dbSNP:529615832
3083 3083 -, tagt dbSNP:77130641
3089 3089 -, g dbSNP:550667141
3090 3090 g, t dbSNP:189060518
3104 3104 -, tttttt dbSNP:775488489
3104 3104 -, ttttt dbSNP:749119807
3104 3104 -, tttt dbSNP:774018291
3106 3106 -, tttt dbSNP:548548539
3155 3155 c, g dbSNP:577801051
3181 3181 c, t dbSNP:560591212
3186 3186 c, g dbSNP:541063831
3190 3190 a, g dbSNP:139684774
3198 3198 a, g dbSNP:762493910
3233 3233 a, g dbSNP:10063
3245 3245 a, g dbSNP:184660941
3296 3296 -, ac dbSNP:746629400
3313 3313 c, t dbSNP:576015374
3315 3315 c, t dbSNP:556327133
3335 3335 a, g dbSNP:542423464
3339 3339 a, t dbSNP:192681806
3413 3413 g, t dbSNP:549304062
3417 3417 -, ttac dbSNP:369296635
3418 3418 -, tact dbSNP:144681167
3430 3430 a, c dbSNP:553628412
3440 3440 a, c dbSNP:563908266
3517 3517 a, c dbSNP:371153381
3552 3552 -, aa dbSNP:34525911

Target ORF information:

RefSeq Version NM_003183
Organism Homo sapiens (human)
Definition Homo sapiens ADAM metallopeptidase domain 17 (ADAM17), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_003183

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGAGGCAGT CTCTCCTATT CCTGACCAGC GTGGTTCCTT TCGTGCTGGC GCCGCGACCT 
CCGGATGACC CGGGCTTCGG CCCCCACCAG AGACTCGAGA AGCTTGATTC TTTGCTCTCA
GACTACGATA TTCTCTCTTT ATCTAATATC CAGCAGCATT CGGTAAGAAA AAGAGATCTA
CAGACTTCAA CACATGTAGA AACACTACTA ACTTTTTCAG CTTTGAAAAG GCATTTTAAA
TTATACCTGA CATCAAGTAC TGAACGTTTT TCACAAAATT TCAAGGTCGT GGTGGTGGAT
GGTAAAAACG AAAGCGAGTA CACTGTAAAA TGGCAGGACT TCTTCACTGG ACACGTGGTT
GGTGAGCCTG ACTCTAGGGT TCTAGCCCAC ATAAGAGATG ATGATGTTAT AATCAGAATC
AACACAGATG GGGCCGAATA TAACATAGAG CCACTTTGGA GATTTGTTAA TGATACCAAA
GACAAAAGAA TGTTAGTTTA TAAATCTGAA GATATCAAGA ATGTTTCACG TTTGCAGTCT
CCAAAAGTGT GTGGTTATTT AAAAGTGGAT AATGAAGAGT TGCTCCCAAA AGGGTTAGTA
GACAGAGAAC CACCTGAAGA GCTTGTTCAT CGAGTGAAAA GAAGAGCTGA CCCAGATCCC
ATGAAGAACA CGTGTAAATT ATTGGTGGTA GCAGATCATC GCTTCTACAG ATACATGGGC
AGAGGGGAAG AGAGTACAAC TACAAATTAC TTAATAGAGC TAATTGACAG AGTTGATGAC
ATCTATCGGA ACACTTCATG GGATAATGCA GGTTTTAAAG GCTATGGAAT ACAGATAGAG
CAGATTCGCA TTCTCAAGTC TCCACAAGAG GTAAAACCTG GTGAAAAGCA CTACAACATG
GCAAAAAGTT ACCCAAATGA AGAAAAGGAT GCTTGGGATG TGAAGATGTT GCTAGAGCAA
TTTAGCTTTG ATATAGCTGA GGAAGCATCT AAAGTTTGCT TGGCACACCT TTTCACATAC
CAAGATTTTG ATATGGGAAC TCTTGGATTA GCTTATGTTG GCTCTCCCAG AGCAAACAGC
CATGGAGGTG TTTGTCCAAA GGCTTATTAT AGCCCAGTTG GGAAGAAAAA TATCTATTTG
AATAGTGGTT TGACGAGCAC AAAGAATTAT GGTAAAACCA TCCTTACAAA GGAAGCTGAC
CTGGTTACAA CTCATGAATT GGGACATAAT TTTGGAGCAG AACATGATCC GGATGGTCTA
GCAGAATGTG CCCCGAATGA GGACCAGGGA GGGAAATATG TCATGTATCC CATAGCTGTG
AGTGGCGATC ACGAGAACAA TAAGATGTTT TCAAACTGCA GTAAACAATC AATCTATAAG
ACCATTGAAA GTAAGGCCCA GGAGTGTTTT CAAGAACGCA GCAATAAAGT TTGTGGGAAC
TCGAGGGTGG ATGAAGGAGA AGAGTGTGAT CCTGGCATCA TGTATCTGAA CAACGACACC
TGCTGCAACA GCGACTGCAC GTTGAAGGAA GGTGTCCAGT GCAGTGACAG GAACAGTCCT
TGCTGTAAAA ACTGTCAGTT TGAGACTGCC CAGAAGAAGT GCCAGGAGGC GATTAATGCT
ACTTGCAAAG GCGTGTCCTA CTGCACAGGT AATAGCAGTG AGTGCCCGCC TCCAGGAAAT
GCTGAAGATG ACACTGTTTG CTTGGATCTT GGCAAGTGTA AGGATGGGAA ATGCATCCCT
TTCTGCGAGA GGGAACAGCA GCTGGAGTCC TGTGCATGTA ATGAAACTGA CAACTCCTGC
AAGGTGTGCT GCAGGGACCT TTCTGGCCGC TGTGTGCCCT ATGTCGATGC TGAACAAAAG
AACTTATTTT TGAGGAAAGG AAAGCCCTGT ACAGTAGGAT TTTGTGACAT GAATGGCAAA
TGTGAGAAAC GAGTACAGGA TGTAATTGAA CGATTTTGGG ATTTCATTGA CCAGCTGAGC
ATCAATACTT TTGGAAAGTT TTTAGCAGAC AACATCGTTG GGTCTGTCCT GGTTTTCTCC
TTGATATTTT GGATTCCTTT CAGCATTCTT GTCCATTGTG TGGATAAGAA ATTGGATAAA
CAGTATGAAT CTCTGTCTCT GTTTCACCCC AGTAACGTCG AAATGCTGAG CAGCATGGAT
TCTGCATCGG TTCGCATTAT CAAACCCTTT CCTGCGCCCC AGACTCCAGG CCGCCTGCAG
CCTGCCCCTG TGATCCCTTC GGCGCCAGCA GCTCCAAAAC TGGACCACCA GAGAATGGAC
ACCATCCAGG AAGACCCCAG CACAGACTCA CATATGGACG AGGATGGGTT TGAGAAGGAC
CCCTTCCCAA ATAGCAGCAC AGCTGCCAAG TCATTTGAGG ATCTCACGGA CCATCCGGTC
ACCAGAAGTG AAAAGGCTGC CTCCTTTAAA CTGCAGCGTC AGAATCGTGT TGACAGCAAA
GAAACAGAGT GCTAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.