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TBX1 T-box 1 [Homo sapiens (human)]

Gene Symbol TBX1
Entrez Gene ID 6899
Full Name T-box 1
Synonyms CAFS, CATCH22, CTHM, DGCR, DGS, DORV, TBX1C, TGA, VCF, VCFS
General protein information
Preferred Names
T-box transcription factor TBX1
Names
T-box transcription factor TBX1
brachyury
Testis-specific T-box protein
T-box 1 transcription factor C
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

22

22q11.21

Summary This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product shares 98% amino acid sequence identity with the mouse ortholog. DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS), a common congenital disorder characterized by neural-crest-related developmental defects, has been associated with deletions of chromosome 22q11.2, where this gene has been mapped. Studies using mouse models of DiGeorge syndrome suggest a major role for this gene in the molecular etiology of DGS/VCFS. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

602054

Disorder Html: Conotruncal anomaly face syndrome, 217095 (3); DiGeorge syndrome,
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu19922 NM_080647 Homo sapiens T-box 1 (TBX1), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK Not in stock -1 Starting from $99.00
OHu19962 NM_080646 Homo sapiens T-box 1 (TBX1), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK Not in stock -1 Starting from $99.00
OHu20328 NM_005992 Homo sapiens T-box 1 (TBX1), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK Not in stock -1 Starting from $99.00
OHu60863 XM_011530351 PREDICTED: Homo sapiens T-box 1 (TBX1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK Not in stock -1 Starting from $99.00
OHu19922 XM_006724312 PREDICTED: Homo sapiens T-box 1 (TBX1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK Not in stock -1 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu19922D
Sequence Information ORF Nucleotide Sequence (Length: 1488bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 04-MAY-2014
Organism Homo sapiens (human)
Product T-box transcription factor TBX1 isoform C
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF373867.1. This sequence is a reference standard in the RefSeqGene project. Summary: This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product shares 98% amino acid sequence identity with the mouse ortholog. DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS), a common congenital disorder characterized by neural-crest-related developmental defects, has been associated with deletions of chromosome 22q11.2, where this gene has been mapped. Studies using mouse models of DiGeorge syndrome suggest a major role for this gene in the molecular etiology of DGS/VCFS. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (C) encodes the longest isoform (C) with the same N-terminal 336 aa, but an unique C-terminus with respect to isoforms A and B. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF373867.1 [ECO:0000332] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCACTTCA GCACCGTCAC CAGGGACATG GAAGCCTTCA CGGCCAGCAG CCTGAGCAGC 
CTGGGGGCCG CGGGGGGCTT CCCGGGCGCC GCGTCGCCCG GCGCCGACCC GTACGGCCCG
CGCGAGCCCC CGCCGCCGCC GCCGCGCTAC GACCCGTGCG CCGCCGCCGC CCCCGGCGCC
CCGGGCCCGC CGCCGCCGCC GCACGCCTAC CCGTTTGCGC CGGCCGCCGG GGCCGCCACC
AGCGCCGCCG CCGAGCCCGA GGGCCCCGGG GCCAGCTGCG CGGCCGCAGC CAAGGCGCCG
GTGAAGAAGA ACGCGAAGGT GGCCGGTGTG AGCGTGCAGC TAGAGATGAA GGCGCTGTGG
GACGAGTTCA ACCAGCTGGG CACCGAGATG ATCGTCACCA AGGCCGGCAG GCGGATGTTT
CCCACCTTCC AAGTGAAGCT CTTCGGCATG GATCCCATGG CCGACTATAT GCTGCTCATG
GACTTCGTGC CGGTGGACGA TAAGCGCTAC CGGTACGCCT TCCACAGCTC CTCCTGGCTG
GTGGCGGGGA AGGCCGACCC TGCCACGCCA GGCCGCGTGC ACTACCACCC GGACTCGCCT
GCCAAGGGCG CGCAGTGGAT GAAGCAAATC GTGTCCTTCG ACAAGCTCAA GCTGACCAAC
AACCTACTGG ACGACAACGG CCACATTATT CTGAATTCCA TGCACAGATA CCAGCCCCGC
TTCCACGTGG TCTATGTGGA CCCACGCAAA GATAGCGAGA AATATGCCGA GGAGAACTTC
AAAACCTTTG TGTTCGAGGA GACACGATTC ACCGCGGTCA CTGCCTACCA GAACCATCGG
ATCACGCAGC TCAAGATTGC CAGCAATCCC TTCGCGAAAG GCTTCCGGGA CTGTGACCCT
GAGGACTGGC CCCGGAACCA CCGGCCCGGC GCACTGCCGC TCATGAGCGC CTTCGCGCGC
TCGCGGAACC CCGTGGCTTC CCCGACGCAG CCCAGCGGCA CGGAGAAAGA CGCGGCTGAG
GCCCGGCGAG AATTCCAGCG CGACGCGGGC GGGCCAGCAG TGCTCGGGGA CCCGGCGCAT
CCTCCGCAGC TGCTGGCCCG GGTGCTAAGC CCCTCGCTGC CCGGGGCCGG CGGCGCCGGC
GGCTTAGTCC CGCTGCCCGG CGCGCCCGGA GGCCGGCCCA GTCCCCCGAA CCCCGAGCTG
CGCCTGGAGG CGCCCGGCGC ATCGGAGCCG CTGCACCACC ACCCCTACAA ATATCCGGCC
GCCGCCTACG ACCACTATCT CGGGGCCAAG AGCCGGCCGG CGCCCTACCC GCTGCCCGGC
CTGCGTGGCC ACGGCTACCA CCCGCACGCG CATCCGCACC ACCACCACCA CCCCGTGAGT
CCAGCCGCCG CGGCCGCCGC CGCCGCTGCC GCAGCTGCCG CGGCCGCCAA CATGTACTCG
TCGGCCGGAG CCGCGCCGCC CGGCTCCTAC GACTATTGCC CCAGATAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_542378.1
CDS130..1617
Misc Feature(1)454..1026(+)
Misc Feature(2)520..1020(+)
Misc Feature(3)592..867(+)
Exon (1)1..43
Gene:TBX1
Gene Synonym:
Exon (2)44..163
Gene:TBX1
Gene Synonym:
Exon (3)164..539
Gene:TBX1
Gene Synonym:
Exon (4)540..641
Gene:TBX1
Gene Synonym:
Exon (5)642..813
Gene:TBX1
Gene Synonym:
Exon (6)814..969
Gene:TBX1
Gene Synonym:
Exon (7)970..1037
Gene:TBX1
Gene Synonym:
Exon (8)1038..1138
Gene:TBX1
Gene Synonym:
Exon (9)1139..2082
Gene:TBX1
Gene Synonym:
Translation
Position Chain Variation Link
3 3 g, t dbSNP:72646938
38 38 a, g dbSNP:573744392
45 45 c, g dbSNP:737868
49 49 a, g dbSNP:529394856
53 53 a, c dbSNP:548055101
66 66 c, t dbSNP:568262861
87 87 c, t dbSNP:757372720
91 91 c, t dbSNP:72646950
94 94 g, t dbSNP:372793286
96 96 a, g dbSNP:756267776
99 99 g, t dbSNP:778719877
100 100 c, t dbSNP:747929709
104 104 a, g dbSNP:772051850
112 112 c, t dbSNP:773143767
119 119 -, c dbSNP:778833362
122 122 c, t dbSNP:554137987
123 123 a, g dbSNP:770508041
124 124 g, t dbSNP:775957254
125 125 a, c dbSNP:759287049
127 127 a, g dbSNP:41298786
129 129 a, g dbSNP:776065724
135 135 c, t dbSNP:367692432
144 144 a, c, t dbSNP:764497467
151 151 a, g dbSNP:28649236
153 153 a, c, g dbSNP:757654739
189 189 c, t dbSNP:746993005
192 192 g, t dbSNP:770754649
204 204 g, t dbSNP:72646952
221 221 c, t dbSNP:745369694
355 355 a, g dbSNP:769629385
386 386 c, t dbSNP:775295536
388 388 c, g dbSNP:762629442
399 399 a, g dbSNP:769163028
405 405 c, g dbSNP:774556411
408 408 c, t dbSNP:762421862
420 420 c, g dbSNP:767905417
422 422 a, g dbSNP:750570550
425 425 a, c dbSNP:143602782
426 426 a, g dbSNP:72646953
428 428 c, g dbSNP:754087937
429 429 a, g dbSNP:755146299
432 432 -, gaa dbSNP:369050575
450 450 a, g dbSNP:148928907
451 451 g, t dbSNP:375718057
453 453 c, t dbSNP:757290764
456 456 a, t dbSNP:780978730
459 459 c, g dbSNP:745762053
463 463 a, g dbSNP:769434775
466 466 c, g dbSNP:779673447
471 471 a, g dbSNP:541899328
472 472 c, g dbSNP:748995341
474 474 a, g dbSNP:768390778
477 477 a, g dbSNP:533247514
486 486 a, g dbSNP:762071043
492 492 c, t dbSNP:772419022
498 498 c, t dbSNP:773592877
509 509 a, g dbSNP:761127806
523 523 a, g dbSNP:766467654
531 531 a, g dbSNP:753894833
542 542 a, g dbSNP:766405525
549 549 c, t dbSNP:41298814
569 569 c, t dbSNP:754446709
572 572 a, t dbSNP:28939675
573 573 c, t dbSNP:139776757
576 576 c, t dbSNP:142182296
581 581 a, t dbSNP:758045369
582 582 a, t dbSNP:778041960
591 591 c, t dbSNP:41298816
592 592 a, g, t dbSNP:374011293
597 597 c, t dbSNP:746308244
603 603 a, g dbSNP:769796903
604 604 c, t dbSNP:775592712
606 606 c, g dbSNP:763136442
615 615 a, c, t dbSNP:764371266
616 616 a, g dbSNP:368122035
620 620 a, c, t dbSNP:766205381
621 621 a, g, t dbSNP:111754814
625 625 a, g dbSNP:371125236
627 627 c, t dbSNP:538317719
628 628 a, c, g dbSNP:777394492
635 635 a, g dbSNP:754818295
639 639 c, t dbSNP:781496582
640 640 a, c dbSNP:746384751
641 641 a, g dbSNP:770313856
645 645 c, t dbSNP:772390067
660 660 c, t dbSNP:200036203
674 674 c, t dbSNP:759363148
678 678 a, g dbSNP:769418078
684 684 c, t dbSNP:775502763
688 688 c, g dbSNP:762763654
696 696 a, g dbSNP:138724943
700 700 a, g dbSNP:773832715
705 705 c, t dbSNP:761495882
706 706 a, g, t dbSNP:767140968
708 708 a, g dbSNP:375727246
711 711 c, g dbSNP:74315522
719 719 c, t dbSNP:766608075
726 726 a, g dbSNP:754390970
729 729 g, t dbSNP:201107301
734 734 a, g dbSNP:755518264
738 738 c, t dbSNP:779173153
739 739 a, g dbSNP:748232668
740 740 c, t dbSNP:774388985
741 741 a, g, t dbSNP:778071107
759 759 a, c, t dbSNP:141186024
760 760 a, g dbSNP:749275495
762 762 a, g dbSNP:150320937
768 768 c, t dbSNP:546513284
771 771 c, t dbSNP:761323992
774 774 a, g dbSNP:766944540
786 786 a, c dbSNP:772944750
792 792 a, c dbSNP:760195570
793 793 c, t dbSNP:2301558
798 798 a, g dbSNP:754119596
801 801 c, t dbSNP:367657296
802 802 a, g dbSNP:765827737
807 807 c, t dbSNP:149453540
810 810 c, g dbSNP:371829515
813 813 c, t dbSNP:200021644
819 819 c, t dbSNP:750574407
842 842 a, g dbSNP:373539086
843 843 a, g dbSNP:778627866
855 855 c, t dbSNP:201412495
863 863 a, c dbSNP:533732094
876 876 c, t dbSNP:777407225
881 881 a, g dbSNP:746486511
886 886 c, g dbSNP:553807294
896 896 c, t dbSNP:776429167
897 897 c, t dbSNP:759225333
898 898 a, g dbSNP:770305746
922 922 c, t dbSNP:775979935
924 924 c, g, t dbSNP:763559631
925 925 a, c, g dbSNP:144848597
927 927 a, g dbSNP:767591426
941 941 c, t dbSNP:750661825
942 942 c, t dbSNP:61730282
943 943 g, t dbSNP:190302733
944 944 c, t dbSNP:752484290
945 945 a, c, g dbSNP:758417980
951 951 c, t dbSNP:746900412
958 958 a, c dbSNP:770380653
968 968 a, g dbSNP:780800634
972 972 a, c dbSNP:760755800
975 975 a, g dbSNP:766377918
986 986 c, t dbSNP:776751131
992 992 a, g dbSNP:764891073
1005 1005 a, g dbSNP:763843987
1015 1015 a, c dbSNP:751339103
1017 1017 c, g dbSNP:146109202
1021 1021 c, t dbSNP:767553992
1024 1024 c, g dbSNP:750330152
1026 1026 c, t dbSNP:755692915
1030 1030 a, g dbSNP:779659218
1033 1033 a, g dbSNP:748939569
1039 1039 c, t dbSNP:754644643
1043 1043 a, g dbSNP:555522870
1048 1048 c, t dbSNP:752278002
1056 1056 c, t dbSNP:201607803
1057 1057 a, g dbSNP:41298838
1058 1058 c, g dbSNP:747565599
1059 1059 a, c, t dbSNP:558072690
1062 1062 a, g dbSNP:41298840
1063 1063 c, t dbSNP:745951558
1067 1067 c, g dbSNP:769708851
1076 1076 c, g dbSNP:775788782
1078 1078 c, g dbSNP:763231000
1080 1080 c, t dbSNP:772039142
1087 1087 c, g, t dbSNP:773107514
1089 1089 c, t dbSNP:766324089
1094 1094 g, t dbSNP:753660073
1115 1115 c, g dbSNP:759090563
1122 1122 c, t dbSNP:764610884
1148 1148 a, t dbSNP:751917634
1154 1154 a, g dbSNP:549715785
1178 1178 a, g dbSNP:781731042
1187 1187 c, g dbSNP:569581176
1188 1188 a, g dbSNP:13054377
1229 1229 a, g dbSNP:548714133
1261 1261 a, g dbSNP:565927787
1269 1269 c, g dbSNP:111605258
1308 1308 a, c dbSNP:780256549
1310 1310 a, g dbSNP:749558577
1312 1312 a, c dbSNP:768991190
1313 1313 a, c dbSNP:773195263
1316 1316 a, c, t dbSNP:746812421
1317 1317 g, t dbSNP:776659796
1318 1318 a, c dbSNP:72646967
1326 1326 a, g dbSNP:764852265
1327 1327 c, g dbSNP:774951964
1329 1329 a, g dbSNP:762662497
1340 1340 c, g, t dbSNP:557935727
1342 1342 c, t dbSNP:757648761
1344 1344 c, t dbSNP:200135498
1345 1345 a, g dbSNP:750966464
1346 1346 a, g dbSNP:756543718
1347 1347 c, g, t dbSNP:780344405
1350 1350 a, g dbSNP:755047093
1352 1352 c, g, t dbSNP:779281442
1353 1353 a, g dbSNP:770781746
1354 1354 c, g dbSNP:776268518
1355 1355 a, c, g dbSNP:745858331
1357 1357 a, c dbSNP:775040109
1358 1358 c, t dbSNP:762467149
1362 1362 a, g dbSNP:763530229
1367 1367 a, g dbSNP:774028490
1371 1371 c, g dbSNP:761327170
1373 1373 c, g dbSNP:767961877
1378 1378 a, c dbSNP:571884633
1383 1383 c, t dbSNP:756699313
1384 1384 c, t dbSNP:368614435
1388 1388 c, t dbSNP:754328637
1389 1389 a, c, t dbSNP:371557427
1390 1390 a, g dbSNP:779087360
1394 1394 c, t dbSNP:753011413
1399 1399 a, g dbSNP:758517884
1401 1401 a, c dbSNP:781108085
1403 1403 a, g dbSNP:569976109
1408 1408 c, t dbSNP:769522876
1416 1416 c, t dbSNP:574947516
1417 1417 a, g dbSNP:749185545
1424 1424 a, g dbSNP:768181576
1426 1426 c, g dbSNP:773715388
1429 1429 c, g dbSNP:761590228
1430 1430 c, t dbSNP:771606854
1431 1431 a, g, t dbSNP:772860144
1438 1438 c, t dbSNP:201993443
1443 1443 c, g dbSNP:754416536
1445 1445 c, g dbSNP:760098496
1446 1446 a, c, g, t dbSNP:573980571
1447 1447 a, g dbSNP:777873225
1450 1450 c, g dbSNP:751740430
1454 1454 c, g dbSNP:755937050
1459 1459 c, t dbSNP:779779535
1468 1468 c, t dbSNP:749275474
1473 1473 a, g dbSNP:768572721
1474 1474 c, t dbSNP:778539160
1476 1476 c, g dbSNP:747623105
1479 1479 c, g dbSNP:771540171
1482 1482 c, g, t dbSNP:367711718
1485 1485 -, cac dbSNP:767638460
1486 1486 -, caccac dbSNP:753713234
1486 1486 -, cac dbSNP:761822829
1491 1491 c, t dbSNP:771471094
1494 1494 c, t dbSNP:777103863
1500 1500 c, t dbSNP:760188566
1501 1501 c, t dbSNP:765827356
1503 1503 c, g, t dbSNP:753192876
1504 1504 c, g dbSNP:764197422
1510 1510 -, ccagccgcc dbSNP:759569338
1511 1511 -, cagccgccg dbSNP:758290031
1512 1512 a, g dbSNP:553461195
1513 1513 -, gccgccgcg dbSNP:777514486
1515 1515 c, t dbSNP:573376280
1518 1518 a, c dbSNP:751755076
1521 1521 -, gcc dbSNP:756680878
1522 1522 -, gccgccgcc dbSNP:769022249
1522 1522 -, gcc dbSNP:780453322
1523 1523 c, t dbSNP:757452865
1525 1525 a, g dbSNP:766206180
1526 1526 c, t dbSNP:753613632
1527 1527 c, t dbSNP:754705790
1528 1528 -, gccgccgct dbSNP:774489872
1530 1530 c, t dbSNP:72646968
1531 1531 a, g dbSNP:778718533
1533 1533 -, gctgccgca dbSNP:748434309
1533 1533 a, c dbSNP:748075946
1534 1534 -, gctgccgca dbSNP:772030062
1535 1535 c, t dbSNP:757867291
1542 1542 -, gctgccgcg dbSNP:760701410
1542 1542 a, c dbSNP:777313046
1544 1544 a, c dbSNP:746699675
1548 1548 c, t dbSNP:770702512
1552 1552 -, gcc dbSNP:765173550
1563 1563 c, g dbSNP:371506174
1564 1564 c, t dbSNP:746250147
1571 1571 c, t dbSNP:770129913
1574 1574 c, t dbSNP:373987708
1575 1575 c, t dbSNP:775895785
1576 1576 a, g dbSNP:541198585
1577 1577 a, g dbSNP:764132783
1582 1582 a, c, g dbSNP:774386479
1586 1586 a, c, t dbSNP:762076391
1587 1587 g, t dbSNP:750574356
1590 1590 c, g, t dbSNP:754795532
1595 1595 c, t dbSNP:564141057
1596 1596 c, t dbSNP:758325399
1602 1602 c, g dbSNP:777213879
1609 1609 c, t dbSNP:746505468
1610 1610 c, g dbSNP:756769832
1612 1612 a, g dbSNP:780998431
1615 1615 a, t dbSNP:745479292
1621 1621 a, g dbSNP:770080000
1624 1624 c, t dbSNP:775903697
1626 1626 c, t dbSNP:368172043
1630 1630 c, t dbSNP:543378212
1632 1632 c, t dbSNP:774778199
1633 1633 a, g dbSNP:761882175
1636 1636 -, c dbSNP:775581777
1638 1638 c, t dbSNP:371856634
1639 1639 g, t dbSNP:773442513
1640 1640 c, t dbSNP:760927067
1643 1643 c, g dbSNP:764978950
1644 1644 a, g dbSNP:375239385
1650 1650 a, g dbSNP:563290139
1655 1655 g, t dbSNP:752531044
1658 1658 c, t dbSNP:762856074
1659 1659 c, t dbSNP:113153036
1666 1666 c, t dbSNP:751476947
1669 1669 c, t dbSNP:756861421
1709 1709 a, g dbSNP:373863680
1713 1713 c, t dbSNP:528896157
1735 1735 a, g dbSNP:72646969
1769 1769 c, g dbSNP:558979141
1781 1781 -, g dbSNP:41298842
1791 1791 c, t dbSNP:528197589
1811 1811 g, t dbSNP:551528783
1820 1820 a, g dbSNP:571783270
1879 1879 c, t dbSNP:772661629
1881 1881 a, t dbSNP:537729175
1895 1895 a, c dbSNP:551038678
1943 1943 -, g dbSNP:544636729
1947 1947 c, t dbSNP:567642855
1952 1952 g, t dbSNP:72646970
2018 2018 a, g dbSNP:72646971
2020 2020 a, t dbSNP:749619227
2034 2034 a, g dbSNP:769933350
2041 2041 c, t dbSNP:553424250
2044 2044 a, c dbSNP:72646972
2051 2051 a, g dbSNP:779040930

Target ORF information:

RefSeq Version NM_080647
Organism Homo sapiens (human)
Definition Homo sapiens T-box 1 (TBX1), transcript variant C, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_080647

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCACTTCA GCACCGTCAC CAGGGACATG GAAGCCTTCA CGGCCAGCAG CCTGAGCAGC 
CTGGGGGCCG CGGGGGGCTT CCCGGGCGCC GCGTCGCCCG GCGCCGACCC GTACGGCCCG
CGCGAGCCCC CGCCGCCGCC GCCGCGCTAC GACCCGTGCG CCGCCGCCGC CCCCGGCGCC
CCGGGCCCGC CGCCGCCGCC GCACGCCTAC CCGTTTGCGC CGGCCGCCGG GGCCGCCACC
AGCGCCGCCG CCGAGCCCGA GGGCCCCGGG GCCAGCTGCG CGGCCGCAGC CAAGGCGCCG
GTGAAGAAGA ACGCGAAGGT GGCCGGTGTG AGCGTGCAGC TAGAGATGAA GGCGCTGTGG
GACGAGTTCA ACCAGCTGGG CACCGAGATG ATCGTCACCA AGGCCGGCAG GCGGATGTTT
CCCACCTTCC AAGTGAAGCT CTTCGGCATG GATCCCATGG CCGACTATAT GCTGCTCATG
GACTTCGTGC CGGTGGACGA TAAGCGCTAC CGGTACGCCT TCCACAGCTC CTCCTGGCTG
GTGGCGGGGA AGGCCGACCC TGCCACGCCA GGCCGCGTGC ACTACCACCC GGACTCGCCT
GCCAAGGGCG CGCAGTGGAT GAAGCAAATC GTGTCCTTCG ACAAGCTCAA GCTGACCAAC
AACCTACTGG ACGACAACGG CCACATTATT CTGAATTCCA TGCACAGATA CCAGCCCCGC
TTCCACGTGG TCTATGTGGA CCCACGCAAA GATAGCGAGA AATATGCCGA GGAGAACTTC
AAAACCTTTG TGTTCGAGGA GACACGATTC ACCGCGGTCA CTGCCTACCA GAACCATCGG
ATCACGCAGC TCAAGATTGC CAGCAATCCC TTCGCGAAAG GCTTCCGGGA CTGTGACCCT
GAGGACTGGC CCCGGAACCA CCGGCCCGGC GCACTGCCGC TCATGAGCGC CTTCGCGCGC
TCGCGGAACC CCGTGGCTTC CCCGACGCAG CCCAGCGGCA CGGAGAAAGA CGCGGCTGAG
GCCCGGCGAG AATTCCAGCG CGACGCGGGC GGGCCAGCAG TGCTCGGGGA CCCGGCGCAT
CCTCCGCAGC TGCTGGCCCG GGTGCTAAGC CCCTCGCTGC CCGGGGCCGG CGGCGCCGGC
GGCTTAGTCC CGCTGCCCGG CGCGCCCGGA GGCCGGCCCA GTCCCCCGAA CCCCGAGCTG
CGCCTGGAGG CGCCCGGCGC ATCGGAGCCG CTGCACCACC ACCCCTACAA ATATCCGGCC
GCCGCCTACG ACCACTATCT CGGGGCCAAG AGCCGGCCGG CGCCCTACCC GCTGCCCGGC
CTGCGTGGCC ACGGCTACCA CCCGCACGCG CATCCGCACC ACCACCACCA CCCCGTGAGT
CCAGCCGCCG CGGCCGCCGC CGCCGCTGCC GCAGCTGCCG CGGCCGCCAA CATGTACTCG
TCGGCCGGAG CCGCGCCGCC CGGCTCCTAC GACTATTGCC CCAGATAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu19962D
Sequence Information ORF Nucleotide Sequence (Length: 1197bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 04-MAY-2014
Organism Homo sapiens (human)
Product T-box transcription factor TBX1 isoform A
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF012130.1. Summary: This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product shares 98% amino acid sequence identity with the mouse ortholog. DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS), a common congenital disorder characterized by neural-crest-related developmental defects, has been associated with deletions of chromosome 22q11.2, where this gene has been mapped. Studies using mouse models of DiGeorge syndrome suggest a major role for this gene in the molecular etiology of DGS/VCFS. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (A) contains an alternate exon 9 compared to variant C, resulting in an isoform (A) with the same N-terminal 336 aa, but an unique C-terminus with respect to isoforms B and C. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF012130.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACTTCA GCACCGTCAC CAGGGACATG GAAGCCTTCA CGGCCAGCAG CCTGAGCAGC 
CTGGGGGCCG CGGGGGGCTT CCCGGGCGCC GCGTCGCCCG GCGCCGACCC GTACGGCCCG
CGCGAGCCCC CGCCGCCGCC GCCGCGCTAC GACCCGTGCG CCGCCGCCGC CCCCGGCGCC
CCGGGCCCGC CGCCGCCGCC GCACGCCTAC CCGTTTGCGC CGGCCGCCGG GGCCGCCACC
AGCGCCGCCG CCGAGCCCGA GGGCCCCGGG GCCAGCTGCG CGGCCGCAGC CAAGGCGCCG
GTGAAGAAGA ACGCGAAGGT GGCCGGTGTG AGCGTGCAGC TAGAGATGAA GGCGCTGTGG
GACGAGTTCA ACCAGCTGGG CACCGAGATG ATCGTCACCA AGGCCGGCAG GCGGATGTTT
CCCACCTTCC AAGTGAAGCT CTTCGGCATG GATCCCATGG CCGACTATAT GCTGCTCATG
GACTTCGTGC CGGTGGACGA TAAGCGCTAC CGGTACGCCT TCCACAGCTC CTCCTGGCTG
GTGGCGGGGA AGGCCGACCC TGCCACGCCA GGCCGCGTGC ACTACCACCC GGACTCGCCT
GCCAAGGGCG CGCAGTGGAT GAAGCAAATC GTGTCCTTCG ACAAGCTCAA GCTGACCAAC
AACCTACTGG ACGACAACGG CCACATTATT CTGAATTCCA TGCACAGATA CCAGCCCCGC
TTCCACGTGG TCTATGTGGA CCCACGCAAA GATAGCGAGA AATATGCCGA GGAGAACTTC
AAAACCTTTG TGTTCGAGGA GACACGATTC ACCGCGGTCA CTGCCTACCA GAACCATCGG
ATCACGCAGC TCAAGATTGC CAGCAATCCC TTCGCGAAAG GCTTCCGGGA CTGTGACCCT
GAGGACTGGC CCCGGAACCA CCGGCCCGGC GCACTGCCGC TCATGAGCGC CTTCGCGCGC
TCGCGGAACC CCGTGGCTTC CCCGACGCAG CCCAGCGGCA CGGAGAAAGG TGGACATGTC
CTGAAGGACA AGGAAGTGAA AGCTGAGACG TCTAGGAACA CACCAGAGAG AGAAGTGGAG
CTTCTGAGGG ATGCAGGTGG CTGTGTGAAC CTGGGGCTCC CCTGCCCCGC AGAGTGCCAA
CCCTTCAATA CCCAGGGCCT GGTGGCTGGG AGGACCGCAG GTGACCGTCT TTGTTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_542377.1
CDS130..1326
Misc Feature(1)454..1026(+)
Misc Feature(2)520..1020(+)
Misc Feature(3)592..867(+)
Exon (1)1..43
Gene:TBX1
Gene Synonym:
Exon (2)44..163
Gene:TBX1
Gene Synonym:
Exon (3)164..539
Gene:TBX1
Gene Synonym:
Exon (4)540..641
Gene:TBX1
Gene Synonym:
Exon (5)642..813
Gene:TBX1
Gene Synonym:
Exon (6)814..969
Gene:TBX1
Gene Synonym:
Exon (7)970..1037
Gene:TBX1
Gene Synonym:
Exon (8)1038..1138
Gene:TBX1
Gene Synonym:
Exon (9)1139..1465
Gene:TBX1
Gene Synonym:
Translation
Position Chain Variation Link
3 3 g, t dbSNP:72646938
38 38 a, g dbSNP:573744392
45 45 c, g dbSNP:737868
49 49 a, g dbSNP:529394856
53 53 a, c dbSNP:548055101
66 66 c, t dbSNP:568262861
87 87 c, t dbSNP:757372720
91 91 c, t dbSNP:72646950
94 94 g, t dbSNP:372793286
96 96 a, g dbSNP:756267776
99 99 g, t dbSNP:778719877
100 100 c, t dbSNP:747929709
104 104 a, g dbSNP:772051850
112 112 c, t dbSNP:773143767
119 119 -, c dbSNP:778833362
122 122 c, t dbSNP:554137987
123 123 a, g dbSNP:770508041
124 124 g, t dbSNP:775957254
125 125 a, c dbSNP:759287049
127 127 a, g dbSNP:41298786
129 129 a, g dbSNP:776065724
135 135 c, t dbSNP:367692432
144 144 a, c, t dbSNP:764497467
151 151 a, g dbSNP:28649236
153 153 a, c, g dbSNP:757654739
189 189 c, t dbSNP:746993005
192 192 g, t dbSNP:770754649
204 204 g, t dbSNP:72646952
221 221 c, t dbSNP:745369694
355 355 a, g dbSNP:769629385
386 386 c, t dbSNP:775295536
388 388 c, g dbSNP:762629442
399 399 a, g dbSNP:769163028
405 405 c, g dbSNP:774556411
408 408 c, t dbSNP:762421862
420 420 c, g dbSNP:767905417
422 422 a, g dbSNP:750570550
425 425 a, c dbSNP:143602782
426 426 a, g dbSNP:72646953
428 428 c, g dbSNP:754087937
429 429 a, g dbSNP:755146299
432 432 -, gaa dbSNP:369050575
450 450 a, g dbSNP:148928907
451 451 g, t dbSNP:375718057
453 453 c, t dbSNP:757290764
456 456 a, t dbSNP:780978730
459 459 c, g dbSNP:745762053
463 463 a, g dbSNP:769434775
466 466 c, g dbSNP:779673447
471 471 a, g dbSNP:541899328
472 472 c, g dbSNP:748995341
474 474 a, g dbSNP:768390778
477 477 a, g dbSNP:533247514
486 486 a, g dbSNP:762071043
492 492 c, t dbSNP:772419022
498 498 c, t dbSNP:773592877
509 509 a, g dbSNP:761127806
523 523 a, g dbSNP:766467654
531 531 a, g dbSNP:753894833
542 542 a, g dbSNP:766405525
549 549 c, t dbSNP:41298814
569 569 c, t dbSNP:754446709
572 572 a, t dbSNP:28939675
573 573 c, t dbSNP:139776757
576 576 c, t dbSNP:142182296
581 581 a, t dbSNP:758045369
582 582 a, t dbSNP:778041960
591 591 c, t dbSNP:41298816
592 592 a, g, t dbSNP:374011293
597 597 c, t dbSNP:746308244
603 603 a, g dbSNP:769796903
604 604 c, t dbSNP:775592712
606 606 c, g dbSNP:763136442
615 615 a, c, t dbSNP:764371266
616 616 a, g dbSNP:368122035
620 620 a, c, t dbSNP:766205381
621 621 a, g, t dbSNP:111754814
625 625 a, g dbSNP:371125236
627 627 c, t dbSNP:538317719
628 628 a, c, g dbSNP:777394492
635 635 a, g dbSNP:754818295
639 639 c, t dbSNP:781496582
640 640 a, c dbSNP:746384751
641 641 a, g dbSNP:770313856
645 645 c, t dbSNP:772390067
660 660 c, t dbSNP:200036203
674 674 c, t dbSNP:759363148
678 678 a, g dbSNP:769418078
684 684 c, t dbSNP:775502763
688 688 c, g dbSNP:762763654
696 696 a, g dbSNP:138724943
700 700 a, g dbSNP:773832715
705 705 c, t dbSNP:761495882
706 706 a, g, t dbSNP:767140968
708 708 a, g dbSNP:375727246
711 711 c, g dbSNP:74315522
719 719 c, t dbSNP:766608075
726 726 a, g dbSNP:754390970
729 729 g, t dbSNP:201107301
734 734 a, g dbSNP:755518264
738 738 c, t dbSNP:779173153
739 739 a, g dbSNP:748232668
740 740 c, t dbSNP:774388985
741 741 a, g, t dbSNP:778071107
759 759 a, c, t dbSNP:141186024
760 760 a, g dbSNP:749275495
762 762 a, g dbSNP:150320937
768 768 c, t dbSNP:546513284
771 771 c, t dbSNP:761323992
774 774 a, g dbSNP:766944540
786 786 a, c dbSNP:772944750
792 792 a, c dbSNP:760195570
793 793 c, t dbSNP:2301558
798 798 a, g dbSNP:754119596
801 801 c, t dbSNP:367657296
802 802 a, g dbSNP:765827737
807 807 c, t dbSNP:149453540
810 810 c, g dbSNP:371829515
813 813 c, t dbSNP:200021644
819 819 c, t dbSNP:750574407
842 842 a, g dbSNP:373539086
843 843 a, g dbSNP:778627866
855 855 c, t dbSNP:201412495
863 863 a, c dbSNP:533732094
876 876 c, t dbSNP:777407225
881 881 a, g dbSNP:746486511
886 886 c, g dbSNP:553807294
896 896 c, t dbSNP:776429167
897 897 c, t dbSNP:759225333
898 898 a, g dbSNP:770305746
922 922 c, t dbSNP:775979935
924 924 c, g, t dbSNP:763559631
925 925 a, c, g dbSNP:144848597
927 927 a, g dbSNP:767591426
941 941 c, t dbSNP:750661825
942 942 c, t dbSNP:61730282
943 943 g, t dbSNP:190302733
944 944 c, t dbSNP:752484290
945 945 a, c, g dbSNP:758417980
951 951 c, t dbSNP:746900412
958 958 a, c dbSNP:770380653
968 968 a, g dbSNP:780800634
972 972 a, c dbSNP:760755800
975 975 a, g dbSNP:766377918
986 986 c, t dbSNP:776751131
992 992 a, g dbSNP:764891073
1005 1005 a, g dbSNP:763843987
1015 1015 a, c dbSNP:751339103
1017 1017 c, g dbSNP:146109202
1021 1021 c, t dbSNP:767553992
1024 1024 c, g dbSNP:750330152
1026 1026 c, t dbSNP:755692915
1030 1030 a, g dbSNP:779659218
1033 1033 a, g dbSNP:748939569
1039 1039 c, t dbSNP:754644643
1043 1043 a, g dbSNP:555522870
1048 1048 c, t dbSNP:752278002
1056 1056 c, t dbSNP:201607803
1057 1057 a, g dbSNP:41298838
1058 1058 c, g dbSNP:747565599
1059 1059 a, c, t dbSNP:558072690
1062 1062 a, g dbSNP:41298840
1063 1063 c, t dbSNP:745951558
1067 1067 c, g dbSNP:769708851
1076 1076 c, g dbSNP:775788782
1078 1078 c, g dbSNP:763231000
1080 1080 c, t dbSNP:772039142
1087 1087 c, g, t dbSNP:773107514
1089 1089 c, t dbSNP:766324089
1094 1094 g, t dbSNP:753660073
1115 1115 c, g dbSNP:759090563
1122 1122 c, t dbSNP:764610884
1144 1144 c, t dbSNP:758486756
1148 1148 a, t dbSNP:140123922
1152 1152 g, t dbSNP:780153829
1165 1165 g, t dbSNP:773243771
1171 1171 c, g dbSNP:760872844
1178 1178 c, t dbSNP:4819522
1186 1186 a, g dbSNP:776659451
1191 1191 a, g dbSNP:200318249
1193 1193 c, g dbSNP:150417730
1195 1195 a, g dbSNP:751571620
1199 1199 a, g dbSNP:139273521
1205 1205 c, t dbSNP:200361367
1214 1214 c, t dbSNP:749948240
1223 1223 c, t dbSNP:149975157
1224 1224 a, g dbSNP:147647675
1227 1227 c, t dbSNP:753388756
1230 1230 c, t dbSNP:755388851
1231 1231 a, t dbSNP:779223677
1256 1256 c, t dbSNP:748691797
1257 1257 c, t dbSNP:772562018
1270 1270 -, c dbSNP:764051702
1271 1271 c, g dbSNP:537578919
1273 1273 -, ggg dbSNP:751246362
1275 1275 -, ca dbSNP:756985780
1276 1276 a, g dbSNP:747125874
1278 1278 -, tg dbSNP:766903380
1279 1279 a, g dbSNP:770850481
1281 1281 c, t dbSNP:776944741
1284 1284 a, g dbSNP:140564310
1286 1286 a, g dbSNP:768623378
1287 1287 a, c dbSNP:774260467
1291 1291 a, g dbSNP:375380772
1292 1292 c, t dbSNP:144421657
1301 1301 a, g dbSNP:369898376
1304 1304 c, t dbSNP:147834990
1305 1305 c, t dbSNP:372448872
1306 1306 a, c, g dbSNP:5993826
1314 1314 c, t dbSNP:765655371
1315 1315 c, t dbSNP:753046301
1316 1316 a, g dbSNP:207477905
1317 1317 a, t dbSNP:375709966
1322 1322 g, t dbSNP:778348935
1325 1325 a, g dbSNP:747435029
1331 1331 c, t dbSNP:757380916
1334 1334 a, g dbSNP:781111688
1337 1337 c, t dbSNP:72646973
1341 1341 c, t dbSNP:372837457
1347 1347 a, g dbSNP:774350353
1350 1350 a, c, g dbSNP:41298008
1351 1351 a, t dbSNP:370729983
1352 1352 c, t dbSNP:760504147
1359 1359 -, gtc dbSNP:749905463
1361 1361 c, g, t dbSNP:765951294
1362 1362 a, g, t dbSNP:200575065
1363 1363 g, t dbSNP:752279963
1376 1376 c, t dbSNP:758793794
1383 1383 c, g dbSNP:41298010
1389 1389 c, t dbSNP:577134146
1397 1397 c, t dbSNP:545596342
1413 1413 a, g dbSNP:758489369
1421 1421 a, g dbSNP:539417850
1447 1447 g, t dbSNP:5746826
1449 1449 a, g dbSNP:746929383

Target ORF information:

RefSeq Version NM_080646
Organism Homo sapiens (human)
Definition Homo sapiens T-box 1 (TBX1), transcript variant A, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_080646

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACTTCA GCACCGTCAC CAGGGACATG GAAGCCTTCA CGGCCAGCAG CCTGAGCAGC 
CTGGGGGCCG CGGGGGGCTT CCCGGGCGCC GCGTCGCCCG GCGCCGACCC GTACGGCCCG
CGCGAGCCCC CGCCGCCGCC GCCGCGCTAC GACCCGTGCG CCGCCGCCGC CCCCGGCGCC
CCGGGCCCGC CGCCGCCGCC GCACGCCTAC CCGTTTGCGC CGGCCGCCGG GGCCGCCACC
AGCGCCGCCG CCGAGCCCGA GGGCCCCGGG GCCAGCTGCG CGGCCGCAGC CAAGGCGCCG
GTGAAGAAGA ACGCGAAGGT GGCCGGTGTG AGCGTGCAGC TAGAGATGAA GGCGCTGTGG
GACGAGTTCA ACCAGCTGGG CACCGAGATG ATCGTCACCA AGGCCGGCAG GCGGATGTTT
CCCACCTTCC AAGTGAAGCT CTTCGGCATG GATCCCATGG CCGACTATAT GCTGCTCATG
GACTTCGTGC CGGTGGACGA TAAGCGCTAC CGGTACGCCT TCCACAGCTC CTCCTGGCTG
GTGGCGGGGA AGGCCGACCC TGCCACGCCA GGCCGCGTGC ACTACCACCC GGACTCGCCT
GCCAAGGGCG CGCAGTGGAT GAAGCAAATC GTGTCCTTCG ACAAGCTCAA GCTGACCAAC
AACCTACTGG ACGACAACGG CCACATTATT CTGAATTCCA TGCACAGATA CCAGCCCCGC
TTCCACGTGG TCTATGTGGA CCCACGCAAA GATAGCGAGA AATATGCCGA GGAGAACTTC
AAAACCTTTG TGTTCGAGGA GACACGATTC ACCGCGGTCA CTGCCTACCA GAACCATCGG
ATCACGCAGC TCAAGATTGC CAGCAATCCC TTCGCGAAAG GCTTCCGGGA CTGTGACCCT
GAGGACTGGC CCCGGAACCA CCGGCCCGGC GCACTGCCGC TCATGAGCGC CTTCGCGCGC
TCGCGGAACC CCGTGGCTTC CCCGACGCAG CCCAGCGGCA CGGAGAAAGG TGGACATGTC
CTGAAGGACA AGGAAGTGAA AGCTGAGACG TCTAGGAACA CACCAGAGAG AGAAGTGGAG
CTTCTGAGGG ATGCAGGTGG CTGTGTGAAC CTGGGGCTCC CCTGCCCCGC AGAGTGCCAA
CCCTTCAATA CCCAGGGCCT GGTGGCTGGG AGGACCGCAG GTGACCGTCT TTGTTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu20328D
Sequence Information ORF Nucleotide Sequence (Length: 1119bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 04-MAY-2014
Organism Homo sapiens (human)
Product T-box transcription factor TBX1 isoform B
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF012131.1. Summary: This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product shares 98% amino acid sequence identity with the mouse ortholog. DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS), a common congenital disorder characterized by neural-crest-related developmental defects, has been associated with deletions of chromosome 22q11.2, where this gene has been mapped. Studies using mouse models of DiGeorge syndrome suggest a major role for this gene in the molecular etiology of DGS/VCFS. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (B) contains an alternate exon 9 and an additional exon 10 compared to variant C. It encodes an isoform (B) with the same N-terminal 336 aa, but an unique C-terminus with respect to isoforms A and C. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF012131.1 [ECO:0000332] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCACTTCA GCACCGTCAC CAGGGACATG GAAGCCTTCA CGGCCAGCAG CCTGAGCAGC 
CTGGGGGCCG CGGGGGGCTT CCCGGGCGCC GCGTCGCCCG GCGCCGACCC GTACGGCCCG
CGCGAGCCCC CGCCGCCGCC GCCGCGCTAC GACCCGTGCG CCGCCGCCGC CCCCGGCGCC
CCGGGCCCGC CGCCGCCGCC GCACGCCTAC CCGTTTGCGC CGGCCGCCGG GGCCGCCACC
AGCGCCGCCG CCGAGCCCGA GGGCCCCGGG GCCAGCTGCG CGGCCGCAGC CAAGGCGCCG
GTGAAGAAGA ACGCGAAGGT GGCCGGTGTG AGCGTGCAGC TAGAGATGAA GGCGCTGTGG
GACGAGTTCA ACCAGCTGGG CACCGAGATG ATCGTCACCA AGGCCGGCAG GCGGATGTTT
CCCACCTTCC AAGTGAAGCT CTTCGGCATG GATCCCATGG CCGACTATAT GCTGCTCATG
GACTTCGTGC CGGTGGACGA TAAGCGCTAC CGGTACGCCT TCCACAGCTC CTCCTGGCTG
GTGGCGGGGA AGGCCGACCC TGCCACGCCA GGCCGCGTGC ACTACCACCC GGACTCGCCT
GCCAAGGGCG CGCAGTGGAT GAAGCAAATC GTGTCCTTCG ACAAGCTCAA GCTGACCAAC
AACCTACTGG ACGACAACGG CCACATTATT CTGAATTCCA TGCACAGATA CCAGCCCCGC
TTCCACGTGG TCTATGTGGA CCCACGCAAA GATAGCGAGA AATATGCCGA GGAGAACTTC
AAAACCTTTG TGTTCGAGGA GACACGATTC ACCGCGGTCA CTGCCTACCA GAACCATCGG
ATCACGCAGC TCAAGATTGC CAGCAATCCC TTCGCGAAAG GCTTCCGGGA CTGTGACCCT
GAGGACTGGC CCCGGAACCA CCGGCCCGGC GCACTGCCGC TCATGAGCGC CTTCGCGCGC
TCGCGGAACC CCGTGGCTTC CCCGACGCAG CCCAGCGGCA CGGAGAAAGG GCTGGTCACA
GAAGGCTCTG GGCTCCAACC TGGCTTGCTG GACGTGCTCT TGAAGCCCCC AAGTAAGAAG
TCTGAGTCCC TGAGACCACC ACACTGCAAG GACACTTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_005983.1
CDS130..1248
Misc Feature(1)454..1026(+)
Misc Feature(2)520..1020(+)
Misc Feature(3)592..867(+)
Exon (1)1..43
Gene:TBX1
Gene Synonym:
Exon (2)44..163
Gene:TBX1
Gene Synonym:
Exon (3)164..539
Gene:TBX1
Gene Synonym:
Exon (4)540..641
Gene:TBX1
Gene Synonym:
Exon (5)642..813
Gene:TBX1
Gene Synonym:
Exon (6)814..969
Gene:TBX1
Gene Synonym:
Exon (7)970..1037
Gene:TBX1
Gene Synonym:
Exon (8)1038..1138
Gene:TBX1
Gene Synonym:
Exon (9)1139..1258
Gene:TBX1
Gene Synonym:
Exon (10)1259..1538
Gene:TBX1
Gene Synonym:
Translation
Position Chain Variation Link
3 3 g, t dbSNP:72646938
38 38 a, g dbSNP:573744392
45 45 c, g dbSNP:737868
49 49 a, g dbSNP:529394856
53 53 a, c dbSNP:548055101
66 66 c, t dbSNP:568262861
87 87 c, t dbSNP:757372720
91 91 c, t dbSNP:72646950
94 94 g, t dbSNP:372793286
96 96 a, g dbSNP:756267776
99 99 g, t dbSNP:778719877
100 100 c, t dbSNP:747929709
104 104 a, g dbSNP:772051850
112 112 c, t dbSNP:773143767
119 119 -, c dbSNP:778833362
122 122 c, t dbSNP:554137987
123 123 a, g dbSNP:770508041
124 124 g, t dbSNP:775957254
125 125 a, c dbSNP:759287049
127 127 a, g dbSNP:41298786
129 129 a, g dbSNP:776065724
135 135 c, t dbSNP:367692432
144 144 a, c, t dbSNP:764497467
151 151 a, g dbSNP:28649236
153 153 a, c, g dbSNP:757654739
189 189 c, t dbSNP:746993005
192 192 g, t dbSNP:770754649
204 204 g, t dbSNP:72646952
221 221 c, t dbSNP:745369694
355 355 a, g dbSNP:769629385
386 386 c, t dbSNP:775295536
388 388 c, g dbSNP:762629442
399 399 a, g dbSNP:769163028
405 405 c, g dbSNP:774556411
408 408 c, t dbSNP:762421862
420 420 c, g dbSNP:767905417
422 422 a, g dbSNP:750570550
425 425 a, c dbSNP:143602782
426 426 a, g dbSNP:72646953
428 428 c, g dbSNP:754087937
429 429 a, g dbSNP:755146299
432 432 -, gaa dbSNP:369050575
450 450 a, g dbSNP:148928907
451 451 g, t dbSNP:375718057
453 453 c, t dbSNP:757290764
456 456 a, t dbSNP:780978730
459 459 c, g dbSNP:745762053
463 463 a, g dbSNP:769434775
466 466 c, g dbSNP:779673447
471 471 a, g dbSNP:541899328
472 472 c, g dbSNP:748995341
474 474 a, g dbSNP:768390778
477 477 a, g dbSNP:533247514
486 486 a, g dbSNP:762071043
492 492 c, t dbSNP:772419022
498 498 c, t dbSNP:773592877
509 509 a, g dbSNP:761127806
523 523 a, g dbSNP:766467654
531 531 a, g dbSNP:753894833
542 542 a, g dbSNP:766405525
549 549 c, t dbSNP:41298814
569 569 c, t dbSNP:754446709
572 572 a, t dbSNP:28939675
573 573 c, t dbSNP:139776757
576 576 c, t dbSNP:142182296
581 581 a, t dbSNP:758045369
582 582 a, t dbSNP:778041960
591 591 c, t dbSNP:41298816
592 592 a, g, t dbSNP:374011293
597 597 c, t dbSNP:746308244
603 603 a, g dbSNP:769796903
604 604 c, t dbSNP:775592712
606 606 c, g dbSNP:763136442
615 615 a, c, t dbSNP:764371266
616 616 a, g dbSNP:368122035
620 620 a, c, t dbSNP:766205381
621 621 a, g, t dbSNP:111754814
625 625 a, g dbSNP:371125236
627 627 c, t dbSNP:538317719
628 628 a, c, g dbSNP:777394492
635 635 a, g dbSNP:754818295
639 639 c, t dbSNP:781496582
640 640 a, c dbSNP:746384751
641 641 a, g dbSNP:770313856
645 645 c, t dbSNP:772390067
660 660 c, t dbSNP:200036203
674 674 c, t dbSNP:759363148
678 678 a, g dbSNP:769418078
684 684 c, t dbSNP:775502763
688 688 c, g dbSNP:762763654
696 696 a, g dbSNP:138724943
700 700 a, g dbSNP:773832715
705 705 c, t dbSNP:761495882
706 706 a, g, t dbSNP:767140968
708 708 a, g dbSNP:375727246
711 711 c, g dbSNP:74315522
719 719 c, t dbSNP:766608075
726 726 a, g dbSNP:754390970
729 729 g, t dbSNP:201107301
734 734 a, g dbSNP:755518264
738 738 c, t dbSNP:779173153
739 739 a, g dbSNP:748232668
740 740 c, t dbSNP:774388985
741 741 a, g, t dbSNP:778071107
759 759 a, c, t dbSNP:141186024
760 760 a, g dbSNP:749275495
762 762 a, g dbSNP:150320937
768 768 c, t dbSNP:546513284
771 771 c, t dbSNP:761323992
774 774 a, g dbSNP:766944540
786 786 a, c dbSNP:772944750
792 792 a, c dbSNP:760195570
793 793 c, t dbSNP:2301558
798 798 a, g dbSNP:754119596
801 801 c, t dbSNP:367657296
802 802 a, g dbSNP:765827737
807 807 c, t dbSNP:149453540
810 810 c, g dbSNP:371829515
813 813 c, t dbSNP:200021644
819 819 c, t dbSNP:750574407
842 842 a, g dbSNP:373539086
843 843 a, g dbSNP:778627866
855 855 c, t dbSNP:201412495
863 863 a, c dbSNP:533732094
876 876 c, t dbSNP:777407225
881 881 a, g dbSNP:746486511
886 886 c, g dbSNP:553807294
896 896 c, t dbSNP:776429167
897 897 c, t dbSNP:759225333
898 898 a, g dbSNP:770305746
922 922 c, t dbSNP:775979935
924 924 c, g, t dbSNP:763559631
925 925 a, c, g dbSNP:144848597
927 927 a, g dbSNP:767591426
941 941 c, t dbSNP:750661825
942 942 c, t dbSNP:61730282
943 943 g, t dbSNP:190302733
944 944 c, t dbSNP:752484290
945 945 a, c, g dbSNP:758417980
951 951 c, t dbSNP:746900412
958 958 a, c dbSNP:770380653
968 968 a, g dbSNP:780800634
972 972 a, c dbSNP:760755800
975 975 a, g dbSNP:766377918
986 986 c, t dbSNP:776751131
992 992 a, g dbSNP:764891073
1005 1005 a, g dbSNP:763843987
1015 1015 a, c dbSNP:751339103
1017 1017 c, g dbSNP:146109202
1021 1021 c, t dbSNP:767553992
1024 1024 c, g dbSNP:750330152
1026 1026 c, t dbSNP:755692915
1030 1030 a, g dbSNP:779659218
1033 1033 a, g dbSNP:748939569
1039 1039 c, t dbSNP:754644643
1043 1043 a, g dbSNP:555522870
1048 1048 c, t dbSNP:752278002
1056 1056 c, t dbSNP:201607803
1057 1057 a, g dbSNP:41298838
1058 1058 c, g dbSNP:747565599
1059 1059 a, c, t dbSNP:558072690
1062 1062 a, g dbSNP:41298840
1063 1063 c, t dbSNP:745951558
1067 1067 c, g dbSNP:769708851
1076 1076 c, g dbSNP:775788782
1078 1078 c, g dbSNP:763231000
1080 1080 c, t dbSNP:772039142
1087 1087 c, g, t dbSNP:773107514
1089 1089 c, t dbSNP:766324089
1094 1094 g, t dbSNP:753660073
1115 1115 c, g dbSNP:759090563
1122 1122 c, t dbSNP:764610884
1139 1139 a, g dbSNP:180794829
1148 1148 c, t dbSNP:772848857
1150 1150 a, c, g dbSNP:537289976
1155 1155 c, t dbSNP:776573713
1157 1157 c, t dbSNP:759061263
1160 1160 a, g dbSNP:769245031
1162 1162 c, t dbSNP:781230731
1182 1182 c, t dbSNP:41298848
1183 1183 a, g dbSNP:375794471
1200 1200 a, t dbSNP:764567301
1202 1202 a, g dbSNP:766688206
1221 1221 c, g dbSNP:751853455
1222 1222 a, c dbSNP:762446325
1223 1223 g, t dbSNP:767911272
1228 1228 a, c dbSNP:750898232
1230 1230 a, c dbSNP:201777905
1231 1231 c, g, t dbSNP:756297500
1250 1250 c, g dbSNP:754165105
1251 1251 a, g dbSNP:755144560
1252 1252 c, t dbSNP:777692145
1253 1253 a, c dbSNP:746785329
1308 1308 a, g dbSNP:9680615
1321 1321 a, t dbSNP:756134092
1361 1361 a, c dbSNP:577173209
1388 1388 a, g dbSNP:191002666
1395 1395 c, t dbSNP:41298852
1405 1405 a, g dbSNP:556984000
1411 1411 c, t dbSNP:573675357
1418 1418 c, t dbSNP:543184679
1424 1424 a, g dbSNP:756991054
1485 1485 -, c dbSNP:572240649
1486 1486 a, c dbSNP:41258424
1497 1497 c, t dbSNP:41298854
1516 1516 -, aaac dbSNP:60613692

Target ORF information:

RefSeq Version NM_005992
Organism Homo sapiens (human)
Definition Homo sapiens T-box 1 (TBX1), transcript variant B, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_005992

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCACTTCA GCACCGTCAC CAGGGACATG GAAGCCTTCA CGGCCAGCAG CCTGAGCAGC 
CTGGGGGCCG CGGGGGGCTT CCCGGGCGCC GCGTCGCCCG GCGCCGACCC GTACGGCCCG
CGCGAGCCCC CGCCGCCGCC GCCGCGCTAC GACCCGTGCG CCGCCGCCGC CCCCGGCGCC
CCGGGCCCGC CGCCGCCGCC GCACGCCTAC CCGTTTGCGC CGGCCGCCGG GGCCGCCACC
AGCGCCGCCG CCGAGCCCGA GGGCCCCGGG GCCAGCTGCG CGGCCGCAGC CAAGGCGCCG
GTGAAGAAGA ACGCGAAGGT GGCCGGTGTG AGCGTGCAGC TAGAGATGAA GGCGCTGTGG
GACGAGTTCA ACCAGCTGGG CACCGAGATG ATCGTCACCA AGGCCGGCAG GCGGATGTTT
CCCACCTTCC AAGTGAAGCT CTTCGGCATG GATCCCATGG CCGACTATAT GCTGCTCATG
GACTTCGTGC CGGTGGACGA TAAGCGCTAC CGGTACGCCT TCCACAGCTC CTCCTGGCTG
GTGGCGGGGA AGGCCGACCC TGCCACGCCA GGCCGCGTGC ACTACCACCC GGACTCGCCT
GCCAAGGGCG CGCAGTGGAT GAAGCAAATC GTGTCCTTCG ACAAGCTCAA GCTGACCAAC
AACCTACTGG ACGACAACGG CCACATTATT CTGAATTCCA TGCACAGATA CCAGCCCCGC
TTCCACGTGG TCTATGTGGA CCCACGCAAA GATAGCGAGA AATATGCCGA GGAGAACTTC
AAAACCTTTG TGTTCGAGGA GACACGATTC ACCGCGGTCA CTGCCTACCA GAACCATCGG
ATCACGCAGC TCAAGATTGC CAGCAATCCC TTCGCGAAAG GCTTCCGGGA CTGTGACCCT
GAGGACTGGC CCCGGAACCA CCGGCCCGGC GCACTGCCGC TCATGAGCGC CTTCGCGCGC
TCGCGGAACC CCGTGGCTTC CCCGACGCAG CCCAGCGGCA CGGAGAAAGG GCTGGTCACA
GAAGGCTCTG GGCTCCAACC TGGCTTGCTG GACGTGCTCT TGAAGCCCCC AAGTAAGAAG
TCTGAGTCCC TGAGACCACC ACACTGCAAG GACACTTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu60863D
Sequence Information ORF Nucleotide Sequence (Length: 1515bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product T-box transcription factor TBX1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGATCTCCG CCGTGTCCAG CCCGTGGCTC ACGCAGCTCT CGCATTTCTG CGACGTTGCA 
GCCTTCACGG CCAGCAGCCT GAGCAGCCTG GGGGCCGCGG GGGGCTTCCC GGGCGCCGCG
TCGCCCGGCG CCGACCCGTA CGGCCCGCGC GAGCCCCCGC CGCCGCCGCC GCGCTACGAC
CCGTGCGCCG CCGCCGCCCC CGGCGCCCCG GGCCCGCCGC CGCCGCCGCA CGCCTACCCG
TTTGCGCCGG CCGCCGGGGC CGCCACCAGC GCCGCCGCCG AGCCCGAGGG CCCCGGGGCC
AGCTGCGCGG CCGCAGCCAA GGCGCCGGTG AAGAAGAACG CGAAGGTGGC CGGTGTGAGC
GTGCAGCTAG AGATGAAGGC GCTGTGGGAC GAGTTCAACC AGCTGGGCAC CGAGATGATC
GTCACCAAGG CCGGCAGGCG GATGTTTCCC ACCTTCCAAG TGAAGCTCTT CGGCATGGAT
CCCATGGCCG ACTATATGCT GCTCATGGAC TTCGTGCCGG TGGACGATAA GCGCTACCGG
TACGCCTTCC ACAGCTCCTC CTGGCTGGTG GCGGGGAAGG CCGACCCTGC CACGCCAGGC
CGCGTGCACT ACCACCCGGA CTCGCCTGCC AAGGGCGCGC AGTGGATGAA GCAAATCGTG
TCCTTCGACA AGCTCAAGCT GACCAACAAC CTACTGGACG ACAACGGCCA CATTATTCTG
AATTCCATGC ACAGATACCA GCCCCGCTTC CACGTGGTCT ATGTGGACCC ACGCAAAGAT
AGCGAGAAAT ATGCCGAGGA GAACTTCAAA ACCTTTGTGT TCGAGGAGAC ACGATTCACC
GCGGTCACTG CCTACCAGAA CCATCGGATC ACGCAGCTCA AGATTGCCAG CAATCCCTTC
GCGAAAGGCT TCCGGGACTG TGACCCTGAG GACTGGCCCC GGAACCACCG GCCCGGCGCA
CTGCCGCTCA TGAGCGCCTT CGCGCGCTCG CGGAACCCCG TGGCTTCCCC GACGCAGCCC
AGCGGCACGG AGAAAGACGC GGCTGAGGCC CGGCGAGAAT TCCAGCGCGA CGCGGGCGGG
CCAGCAGTGC TCGGGGACCC GGCGCATCCT CCGCAGCTGC TGGCCCGGGT GCTAAGCCCC
TCGCTGCCCG GGGCCGGCGG CGCCGGCGGC TTAGTCCCGC TGCCCGGCGC GCCCGGAGGC
CGGCCCAGTC CCCCGAACCC CGAGCTGCGC CTGGAGGCGC CCGGCGCATC GGAGCCGCTG
CACCACCACC CCTACAAATA TCCGGCCGCC GCCTACGACC ACTATCTCGG GGCCAAGAGC
CGGCCGGCGC CCTACCCGCT GCCCGGCCTG CGTGGCCACG GCTACCACCC GCACGCGCAT
CCGCACCACC ACCACCACCC CGTGAGTCCA GCCGCCGCGG CCGCCGCCGC CGCTGCCGCA
GCTGCCGCGG CCGCCAACAT GTACTCGTCG GCCGGAGCCG CGCCGCCCGG CTCCTACGAC
TATTGCCCCA GATAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011528653.1
CDS48..1562
Misc Feature(1)399..971(+)
Misc Feature(2)465..965(+)
Misc Feature(3)537..812(+)
Translation
Position Chain Variation Link
134 134 c, t dbSNP:746993005
137 137 g, t dbSNP:770754649
149 149 g, t dbSNP:72646952
166 166 c, t dbSNP:745369694
300 300 a, g dbSNP:769629385
331 331 c, t dbSNP:775295536
333 333 c, g dbSNP:762629442
344 344 a, g dbSNP:769163028
350 350 c, g dbSNP:774556411
353 353 c, t dbSNP:762421862
365 365 c, g dbSNP:767905417
367 367 a, g dbSNP:750570550
370 370 a, c dbSNP:143602782
371 371 a, g dbSNP:72646953
373 373 c, g dbSNP:754087937
374 374 a, g dbSNP:755146299
377 377 -, gaa dbSNP:369050575
395 395 a, g dbSNP:148928907
396 396 g, t dbSNP:375718057
398 398 c, t dbSNP:757290764
401 401 a, t dbSNP:780978730
404 404 c, g dbSNP:745762053
408 408 a, g dbSNP:769434775
411 411 c, g dbSNP:779673447
416 416 a, g dbSNP:541899328
417 417 c, g dbSNP:748995341
419 419 a, g dbSNP:768390778
422 422 a, g dbSNP:533247514
431 431 a, g dbSNP:762071043
437 437 c, t dbSNP:772419022
443 443 c, t dbSNP:773592877
454 454 a, g dbSNP:761127806
468 468 a, g dbSNP:766467654
476 476 a, g dbSNP:753894833
487 487 a, g dbSNP:766405525
494 494 c, t dbSNP:41298814
514 514 c, t dbSNP:754446709
517 517 a, t dbSNP:28939675
518 518 c, t dbSNP:139776757
521 521 c, t dbSNP:142182296
526 526 a, t dbSNP:758045369
527 527 a, t dbSNP:778041960
536 536 c, t dbSNP:41298816
537 537 a, g, t dbSNP:374011293
542 542 c, t dbSNP:746308244
548 548 a, g dbSNP:769796903
549 549 c, t dbSNP:775592712
551 551 c, g dbSNP:763136442
560 560 a, c, t dbSNP:764371266
561 561 a, g dbSNP:368122035
565 565 a, c, t dbSNP:766205381
566 566 a, g, t dbSNP:111754814
570 570 a, g dbSNP:371125236
572 572 c, t dbSNP:538317719
573 573 a, c, g dbSNP:777394492
580 580 a, g dbSNP:754818295
584 584 c, t dbSNP:781496582
585 585 a, c dbSNP:746384751
586 586 a, g dbSNP:770313856
590 590 c, t dbSNP:772390067
605 605 c, t dbSNP:200036203
619 619 c, t dbSNP:759363148
623 623 a, g dbSNP:769418078
629 629 c, t dbSNP:775502763
633 633 c, g dbSNP:762763654
641 641 a, g dbSNP:138724943
645 645 a, g dbSNP:773832715
650 650 c, t dbSNP:761495882
651 651 a, g, t dbSNP:767140968
653 653 a, g dbSNP:375727246
656 656 c, g dbSNP:74315522
664 664 c, t dbSNP:766608075
671 671 a, g dbSNP:754390970
674 674 g, t dbSNP:201107301
679 679 a, g dbSNP:755518264
683 683 c, t dbSNP:779173153
684 684 a, g dbSNP:748232668
685 685 c, t dbSNP:774388985
686 686 a, g, t dbSNP:778071107
704 704 a, c, t dbSNP:141186024
705 705 a, g dbSNP:749275495
707 707 a, g dbSNP:150320937
713 713 c, t dbSNP:546513284
716 716 c, t dbSNP:761323992
719 719 a, g dbSNP:766944540
731 731 a, c dbSNP:772944750
737 737 a, c dbSNP:760195570
738 738 c, t dbSNP:2301558
743 743 a, g dbSNP:754119596
746 746 c, t dbSNP:367657296
747 747 a, g dbSNP:765827737
752 752 c, t dbSNP:149453540
755 755 c, g dbSNP:371829515
758 758 c, t dbSNP:200021644
764 764 c, t dbSNP:750574407
787 787 a, g dbSNP:373539086
788 788 a, g dbSNP:778627866
800 800 c, t dbSNP:201412495
808 808 a, c dbSNP:533732094
821 821 c, t dbSNP:777407225
826 826 a, g dbSNP:746486511
831 831 c, g dbSNP:553807294
841 841 c, t dbSNP:776429167
842 842 c, t dbSNP:759225333
843 843 a, g dbSNP:770305746
867 867 c, t dbSNP:775979935
869 869 c, g, t dbSNP:763559631
870 870 a, c, g dbSNP:144848597
872 872 a, g dbSNP:767591426
886 886 c, t dbSNP:750661825
887 887 c, t dbSNP:61730282
888 888 g, t dbSNP:190302733
889 889 c, t dbSNP:752484290
890 890 a, c, g dbSNP:758417980
896 896 c, t dbSNP:746900412
903 903 a, c dbSNP:770380653
913 913 a, g dbSNP:780800634
917 917 a, c dbSNP:760755800
920 920 a, g dbSNP:766377918
931 931 c, t dbSNP:776751131
937 937 a, g dbSNP:764891073
950 950 a, g dbSNP:763843987
960 960 a, c dbSNP:751339103
962 962 c, g dbSNP:146109202
966 966 c, t dbSNP:767553992
969 969 c, g dbSNP:750330152
971 971 c, t dbSNP:755692915
975 975 a, g dbSNP:779659218
978 978 a, g dbSNP:748939569
984 984 c, t dbSNP:754644643
988 988 a, g dbSNP:555522870
993 993 c, t dbSNP:752278002
1001 1001 c, t dbSNP:201607803
1002 1002 a, g dbSNP:41298838
1003 1003 c, g dbSNP:747565599
1004 1004 a, c, t dbSNP:558072690
1007 1007 a, g dbSNP:41298840
1008 1008 c, t dbSNP:745951558
1012 1012 c, g dbSNP:769708851
1021 1021 c, g dbSNP:775788782
1023 1023 c, g dbSNP:763231000
1025 1025 c, t dbSNP:772039142
1032 1032 c, g, t dbSNP:773107514
1034 1034 c, t dbSNP:766324089
1039 1039 g, t dbSNP:753660073
1060 1060 c, g dbSNP:759090563
1067 1067 c, t dbSNP:764610884
1093 1093 a, t dbSNP:751917634
1099 1099 a, g dbSNP:549715785
1123 1123 a, g dbSNP:781731042
1132 1132 c, g dbSNP:569581176
1133 1133 a, g dbSNP:13054377
1174 1174 a, g dbSNP:548714133
1206 1206 a, g dbSNP:565927787
1214 1214 c, g dbSNP:111605258
1253 1253 a, c dbSNP:780256549
1255 1255 a, g dbSNP:749558577
1257 1257 a, c dbSNP:768991190
1258 1258 a, c dbSNP:773195263
1261 1261 a, c, t dbSNP:746812421
1262 1262 g, t dbSNP:776659796
1263 1263 a, c dbSNP:72646967
1271 1271 a, g dbSNP:764852265
1272 1272 c, g dbSNP:774951964
1274 1274 a, g dbSNP:762662497
1285 1285 c, g, t dbSNP:557935727
1287 1287 c, t dbSNP:757648761
1289 1289 c, t dbSNP:200135498
1290 1290 a, g dbSNP:750966464
1291 1291 a, g dbSNP:756543718
1292 1292 c, g, t dbSNP:780344405
1295 1295 a, g dbSNP:755047093
1297 1297 c, g, t dbSNP:779281442
1298 1298 a, g dbSNP:770781746
1299 1299 c, g dbSNP:776268518
1300 1300 a, c, g dbSNP:745858331
1302 1302 a, c dbSNP:775040109
1303 1303 c, t dbSNP:762467149
1307 1307 a, g dbSNP:763530229
1312 1312 a, g dbSNP:774028490
1316 1316 c, g dbSNP:761327170
1318 1318 c, g dbSNP:767961877
1323 1323 a, c dbSNP:571884633
1328 1328 c, t dbSNP:756699313
1329 1329 c, t dbSNP:368614435
1333 1333 c, t dbSNP:754328637
1334 1334 a, c, t dbSNP:371557427
1335 1335 a, g dbSNP:779087360
1339 1339 c, t dbSNP:753011413
1344 1344 a, g dbSNP:758517884
1346 1346 a, c dbSNP:781108085
1348 1348 a, g dbSNP:569976109
1353 1353 c, t dbSNP:769522876
1361 1361 c, t dbSNP:574947516
1362 1362 a, g dbSNP:749185545
1369 1369 a, g dbSNP:768181576
1371 1371 c, g dbSNP:773715388
1374 1374 c, g dbSNP:761590228
1375 1375 c, t dbSNP:771606854
1376 1376 a, g, t dbSNP:772860144
1383 1383 c, t dbSNP:201993443
1388 1388 c, g dbSNP:754416536
1390 1390 c, g dbSNP:760098496
1391 1391 a, c, g, t dbSNP:573980571
1392 1392 a, g dbSNP:777873225
1395 1395 c, g dbSNP:751740430
1399 1399 c, g dbSNP:755937050
1404 1404 c, t dbSNP:779779535
1413 1413 c, t dbSNP:749275474
1418 1418 a, g dbSNP:768572721
1419 1419 c, t dbSNP:778539160
1421 1421 c, g dbSNP:747623105
1424 1424 c, g dbSNP:771540171
1427 1427 c, g, t dbSNP:367711718
1430 1430 -, cac dbSNP:767638460
1431 1431 -, caccac dbSNP:753713234
1431 1431 -, cac dbSNP:761822829
1436 1436 c, t dbSNP:771471094
1439 1439 c, t dbSNP:777103863
1445 1445 c, t dbSNP:760188566
1446 1446 c, t dbSNP:765827356
1448 1448 c, g, t dbSNP:753192876
1449 1449 c, g dbSNP:764197422
1455 1455 -, ccagccgcc dbSNP:759569338
1456 1456 -, cagccgccg dbSNP:758290031
1457 1457 a, g dbSNP:553461195
1458 1458 -, gccgccgcg dbSNP:777514486
1460 1460 c, t dbSNP:573376280
1463 1463 a, c dbSNP:751755076
1466 1466 -, gcc dbSNP:756680878
1467 1467 -, gccgccgcc dbSNP:769022249
1467 1467 -, gcc dbSNP:780453322
1468 1468 c, t dbSNP:757452865
1470 1470 a, g dbSNP:766206180
1471 1471 c, t dbSNP:753613632
1472 1472 c, t dbSNP:754705790
1473 1473 -, gccgccgct dbSNP:774489872
1475 1475 c, t dbSNP:72646968
1476 1476 a, g dbSNP:778718533
1478 1478 -, gctgccgca dbSNP:748434309
1478 1478 a, c dbSNP:748075946
1479 1479 -, gctgccgca dbSNP:772030062
1480 1480 c, t dbSNP:757867291
1487 1487 -, gctgccgcg dbSNP:760701410
1487 1487 a, c dbSNP:777313046
1489 1489 a, c dbSNP:746699675
1493 1493 c, t dbSNP:770702512
1497 1497 -, gcc dbSNP:765173550
1508 1508 c, g dbSNP:371506174
1509 1509 c, t dbSNP:746250147
1516 1516 c, t dbSNP:770129913
1519 1519 c, t dbSNP:373987708
1520 1520 c, t dbSNP:775895785
1521 1521 a, g dbSNP:541198585
1522 1522 a, g dbSNP:764132783
1527 1527 a, c, g dbSNP:774386479
1531 1531 a, c, t dbSNP:762076391
1532 1532 g, t dbSNP:750574356
1535 1535 c, g, t dbSNP:754795532
1540 1540 c, t dbSNP:564141057
1541 1541 c, t dbSNP:758325399
1547 1547 c, g dbSNP:777213879
1554 1554 c, t dbSNP:746505468
1555 1555 c, g dbSNP:756769832
1557 1557 a, g dbSNP:780998431
1560 1560 a, t dbSNP:745479292
1566 1566 a, g dbSNP:770080000
1569 1569 c, t dbSNP:775903697
1571 1571 c, t dbSNP:368172043
1575 1575 c, t dbSNP:543378212
1577 1577 c, t dbSNP:774778199
1578 1578 a, g dbSNP:761882175
1581 1581 -, c dbSNP:775581777
1583 1583 c, t dbSNP:371856634
1584 1584 g, t dbSNP:773442513
1585 1585 c, t dbSNP:760927067
1588 1588 c, g dbSNP:764978950
1589 1589 a, g dbSNP:375239385
1595 1595 a, g dbSNP:563290139
1600 1600 g, t dbSNP:752531044
1603 1603 c, t dbSNP:762856074
1604 1604 c, t dbSNP:113153036
1611 1611 c, t dbSNP:751476947
1614 1614 c, t dbSNP:756861421
1654 1654 a, g dbSNP:373863680
1658 1658 c, t dbSNP:528896157
1680 1680 a, g dbSNP:72646969
1714 1714 c, g dbSNP:558979141
1726 1726 -, g dbSNP:41298842
1736 1736 c, t dbSNP:528197589
1756 1756 g, t dbSNP:551528783
1765 1765 a, g dbSNP:571783270
1824 1824 c, t dbSNP:772661629
1826 1826 a, t dbSNP:537729175
1840 1840 a, c dbSNP:551038678
1888 1888 -, g dbSNP:544636729
1892 1892 c, t dbSNP:567642855
1897 1897 g, t dbSNP:72646970
1963 1963 a, g dbSNP:72646971
1965 1965 a, t dbSNP:749619227
1979 1979 a, g dbSNP:769933350
1986 1986 c, t dbSNP:553424250
1989 1989 a, c dbSNP:72646972
1996 1996 a, g dbSNP:779040930

Target ORF information:

RefSeq Version XM_011530351
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens T-box 1 (TBX1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011530351

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGATCTCCG CCGTGTCCAG CCCGTGGCTC ACGCAGCTCT CGCATTTCTG CGACGTTGCA 
GCCTTCACGG CCAGCAGCCT GAGCAGCCTG GGGGCCGCGG GGGGCTTCCC GGGCGCCGCG
TCGCCCGGCG CCGACCCGTA CGGCCCGCGC GAGCCCCCGC CGCCGCCGCC GCGCTACGAC
CCGTGCGCCG CCGCCGCCCC CGGCGCCCCG GGCCCGCCGC CGCCGCCGCA CGCCTACCCG
TTTGCGCCGG CCGCCGGGGC CGCCACCAGC GCCGCCGCCG AGCCCGAGGG CCCCGGGGCC
AGCTGCGCGG CCGCAGCCAA GGCGCCGGTG AAGAAGAACG CGAAGGTGGC CGGTGTGAGC
GTGCAGCTAG AGATGAAGGC GCTGTGGGAC GAGTTCAACC AGCTGGGCAC CGAGATGATC
GTCACCAAGG CCGGCAGGCG GATGTTTCCC ACCTTCCAAG TGAAGCTCTT CGGCATGGAT
CCCATGGCCG ACTATATGCT GCTCATGGAC TTCGTGCCGG TGGACGATAA GCGCTACCGG
TACGCCTTCC ACAGCTCCTC CTGGCTGGTG GCGGGGAAGG CCGACCCTGC CACGCCAGGC
CGCGTGCACT ACCACCCGGA CTCGCCTGCC AAGGGCGCGC AGTGGATGAA GCAAATCGTG
TCCTTCGACA AGCTCAAGCT GACCAACAAC CTACTGGACG ACAACGGCCA CATTATTCTG
AATTCCATGC ACAGATACCA GCCCCGCTTC CACGTGGTCT ATGTGGACCC ACGCAAAGAT
AGCGAGAAAT ATGCCGAGGA GAACTTCAAA ACCTTTGTGT TCGAGGAGAC ACGATTCACC
GCGGTCACTG CCTACCAGAA CCATCGGATC ACGCAGCTCA AGATTGCCAG CAATCCCTTC
GCGAAAGGCT TCCGGGACTG TGACCCTGAG GACTGGCCCC GGAACCACCG GCCCGGCGCA
CTGCCGCTCA TGAGCGCCTT CGCGCGCTCG CGGAACCCCG TGGCTTCCCC GACGCAGCCC
AGCGGCACGG AGAAAGACGC GGCTGAGGCC CGGCGAGAAT TCCAGCGCGA CGCGGGCGGG
CCAGCAGTGC TCGGGGACCC GGCGCATCCT CCGCAGCTGC TGGCCCGGGT GCTAAGCCCC
TCGCTGCCCG GGGCCGGCGG CGCCGGCGGC TTAGTCCCGC TGCCCGGCGC GCCCGGAGGC
CGGCCCAGTC CCCCGAACCC CGAGCTGCGC CTGGAGGCGC CCGGCGCATC GGAGCCGCTG
CACCACCACC CCTACAAATA TCCGGCCGCC GCCTACGACC ACTATCTCGG GGCCAAGAGC
CGGCCGGCGC CCTACCCGCT GCCCGGCCTG CGTGGCCACG GCTACCACCC GCACGCGCAT
CCGCACCACC ACCACCACCC CGTGAGTCCA GCCGCCGCGG CCGCCGCCGC CGCTGCCGCA
GCTGCCGCGG CCGCCAACAT GTACTCGTCG GCCGGAGCCG CGCCGCCCGG CTCCTACGAC
TATTGCCCCA GATAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu19922D
Sequence Information ORF Nucleotide Sequence (Length: 1488bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product T-box transcription factor TBX1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011520.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCACTTCA GCACCGTCAC CAGGGACATG GAAGCCTTCA CGGCCAGCAG CCTGAGCAGC 
CTGGGGGCCG CGGGGGGCTT CCCGGGCGCC GCGTCGCCCG GCGCCGACCC GTACGGCCCG
CGCGAGCCCC CGCCGCCGCC GCCGCGCTAC GACCCGTGCG CCGCCGCCGC CCCCGGCGCC
CCGGGCCCGC CGCCGCCGCC GCACGCCTAC CCGTTTGCGC CGGCCGCCGG GGCCGCCACC
AGCGCCGCCG CCGAGCCCGA GGGCCCCGGG GCCAGCTGCG CGGCCGCAGC CAAGGCGCCG
GTGAAGAAGA ACGCGAAGGT GGCCGGTGTG AGCGTGCAGC TAGAGATGAA GGCGCTGTGG
GACGAGTTCA ACCAGCTGGG CACCGAGATG ATCGTCACCA AGGCCGGCAG GCGGATGTTT
CCCACCTTCC AAGTGAAGCT CTTCGGCATG GATCCCATGG CCGACTATAT GCTGCTCATG
GACTTCGTGC CGGTGGACGA TAAGCGCTAC CGGTACGCCT TCCACAGCTC CTCCTGGCTG
GTGGCGGGGA AGGCCGACCC TGCCACGCCA GGCCGCGTGC ACTACCACCC GGACTCGCCT
GCCAAGGGCG CGCAGTGGAT GAAGCAAATC GTGTCCTTCG ACAAGCTCAA GCTGACCAAC
AACCTACTGG ACGACAACGG CCACATTATT CTGAATTCCA TGCACAGATA CCAGCCCCGC
TTCCACGTGG TCTATGTGGA CCCACGCAAA GATAGCGAGA AATATGCCGA GGAGAACTTC
AAAACCTTTG TGTTCGAGGA GACACGATTC ACCGCGGTCA CTGCCTACCA GAACCATCGG
ATCACGCAGC TCAAGATTGC CAGCAATCCC TTCGCGAAAG GCTTCCGGGA CTGTGACCCT
GAGGACTGGC CCCGGAACCA CCGGCCCGGC GCACTGCCGC TCATGAGCGC CTTCGCGCGC
TCGCGGAACC CCGTGGCTTC CCCGACGCAG CCCAGCGGCA CGGAGAAAGA CGCGGCTGAG
GCCCGGCGAG AATTCCAGCG CGACGCGGGC GGGCCAGCAG TGCTCGGGGA CCCGGCGCAT
CCTCCGCAGC TGCTGGCCCG GGTGCTAAGC CCCTCGCTGC CCGGGGCCGG CGGCGCCGGC
GGCTTAGTCC CGCTGCCCGG CGCGCCCGGA GGCCGGCCCA GTCCCCCGAA CCCCGAGCTG
CGCCTGGAGG CGCCCGGCGC ATCGGAGCCG CTGCACCACC ACCCCTACAA ATATCCGGCC
GCCGCCTACG ACCACTATCT CGGGGCCAAG AGCCGGCCGG CGCCCTACCC GCTGCCCGGC
CTGCGTGGCC ACGGCTACCA CCCGCACGCG CATCCGCACC ACCACCACCA CCCCGTGAGT
CCAGCCGCCG CGGCCGCCGC CGCCGCTGCC GCAGCTGCCG CGGCCGCCAA CATGTACTCG
TCGGCCGGAG CCGCGCCGCC CGGCTCCTAC GACTATTGCC CCAGATAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_006724375.1
CDS304..1791
Misc Feature(1)628..1200(+)
Misc Feature(2)694..1194(+)
Misc Feature(3)766..1041(+)
Translation
Position Chain Variation Link
8 8 c, t dbSNP:778644536
9 9 a, g dbSNP:571964738
20 20 a, g dbSNP:545931815
25 25 c, t dbSNP:563057690
30 30 a, g dbSNP:72646946
34 34 c, t dbSNP:377295286
35 35 a, g dbSNP:72646947
44 44 a, g dbSNP:41299453
60 60 a, g dbSNP:41299455
61 61 a, g dbSNP:772023563
87 87 a, c dbSNP:527989704
97 97 a, g dbSNP:768089174
117 117 -, c dbSNP:758716733
120 120 a, g dbSNP:547445185
136 136 g, t dbSNP:570526602
157 157 a, c, t dbSNP:181490571
158 158 a, g dbSNP:549888507
219 219 c, g dbSNP:737868
223 223 a, g dbSNP:529394856
227 227 a, c dbSNP:548055101
240 240 c, t dbSNP:568262861
261 261 c, t dbSNP:757372720
265 265 c, t dbSNP:72646950
268 268 g, t dbSNP:372793286
270 270 a, g dbSNP:756267776
273 273 g, t dbSNP:778719877
274 274 c, t dbSNP:747929709
278 278 a, g dbSNP:772051850
286 286 c, t dbSNP:773143767
293 293 -, c dbSNP:778833362
296 296 c, t dbSNP:554137987
297 297 a, g dbSNP:770508041
298 298 g, t dbSNP:775957254
299 299 a, c dbSNP:759287049
301 301 a, g dbSNP:41298786
303 303 a, g dbSNP:776065724
309 309 c, t dbSNP:367692432
318 318 a, c, t dbSNP:764497467
325 325 a, g dbSNP:28649236
327 327 a, c, g dbSNP:757654739
363 363 c, t dbSNP:746993005
366 366 g, t dbSNP:770754649
378 378 g, t dbSNP:72646952
395 395 c, t dbSNP:745369694
529 529 a, g dbSNP:769629385
560 560 c, t dbSNP:775295536
562 562 c, g dbSNP:762629442
573 573 a, g dbSNP:769163028
579 579 c, g dbSNP:774556411
582 582 c, t dbSNP:762421862
594 594 c, g dbSNP:767905417
596 596 a, g dbSNP:750570550
599 599 a, c dbSNP:143602782
600 600 a, g dbSNP:72646953
602 602 c, g dbSNP:754087937
603 603 a, g dbSNP:755146299
606 606 -, gaa dbSNP:369050575
624 624 a, g dbSNP:148928907
625 625 g, t dbSNP:375718057
627 627 c, t dbSNP:757290764
630 630 a, t dbSNP:780978730
633 633 c, g dbSNP:745762053
637 637 a, g dbSNP:769434775
640 640 c, g dbSNP:779673447
645 645 a, g dbSNP:541899328
646 646 c, g dbSNP:748995341
648 648 a, g dbSNP:768390778
651 651 a, g dbSNP:533247514
660 660 a, g dbSNP:762071043
666 666 c, t dbSNP:772419022
672 672 c, t dbSNP:773592877
683 683 a, g dbSNP:761127806
697 697 a, g dbSNP:766467654
705 705 a, g dbSNP:753894833
716 716 a, g dbSNP:766405525
723 723 c, t dbSNP:41298814
743 743 c, t dbSNP:754446709
746 746 a, t dbSNP:28939675
747 747 c, t dbSNP:139776757
750 750 c, t dbSNP:142182296
755 755 a, t dbSNP:758045369
756 756 a, t dbSNP:778041960
765 765 c, t dbSNP:41298816
766 766 a, g, t dbSNP:374011293
771 771 c, t dbSNP:746308244
777 777 a, g dbSNP:769796903
778 778 c, t dbSNP:775592712
780 780 c, g dbSNP:763136442
789 789 a, c, t dbSNP:764371266
790 790 a, g dbSNP:368122035
794 794 a, c, t dbSNP:766205381
795 795 a, g, t dbSNP:111754814
799 799 a, g dbSNP:371125236
801 801 c, t dbSNP:538317719
802 802 a, c, g dbSNP:777394492
809 809 a, g dbSNP:754818295
813 813 c, t dbSNP:781496582
814 814 a, c dbSNP:746384751
815 815 a, g dbSNP:770313856
819 819 c, t dbSNP:772390067
834 834 c, t dbSNP:200036203
848 848 c, t dbSNP:759363148
852 852 a, g dbSNP:769418078
858 858 c, t dbSNP:775502763
862 862 c, g dbSNP:762763654
870 870 a, g dbSNP:138724943
874 874 a, g dbSNP:773832715
879 879 c, t dbSNP:761495882
880 880 a, g, t dbSNP:767140968
882 882 a, g dbSNP:375727246
885 885 c, g dbSNP:74315522
893 893 c, t dbSNP:766608075
900 900 a, g dbSNP:754390970
903 903 g, t dbSNP:201107301
908 908 a, g dbSNP:755518264
912 912 c, t dbSNP:779173153
913 913 a, g dbSNP:748232668
914 914 c, t dbSNP:774388985
915 915 a, g, t dbSNP:778071107
933 933 a, c, t dbSNP:141186024
934 934 a, g dbSNP:749275495
936 936 a, g dbSNP:150320937
942 942 c, t dbSNP:546513284
945 945 c, t dbSNP:761323992
948 948 a, g dbSNP:766944540
960 960 a, c dbSNP:772944750
966 966 a, c dbSNP:760195570
967 967 c, t dbSNP:2301558
972 972 a, g dbSNP:754119596
975 975 c, t dbSNP:367657296
976 976 a, g dbSNP:765827737
981 981 c, t dbSNP:149453540
984 984 c, g dbSNP:371829515
987 987 c, t dbSNP:200021644
993 993 c, t dbSNP:750574407
1016 1016 a, g dbSNP:373539086
1017 1017 a, g dbSNP:778627866
1029 1029 c, t dbSNP:201412495
1037 1037 a, c dbSNP:533732094
1050 1050 c, t dbSNP:777407225
1055 1055 a, g dbSNP:746486511
1060 1060 c, g dbSNP:553807294
1070 1070 c, t dbSNP:776429167
1071 1071 c, t dbSNP:759225333
1072 1072 a, g dbSNP:770305746
1096 1096 c, t dbSNP:775979935
1098 1098 c, g, t dbSNP:763559631
1099 1099 a, c, g dbSNP:144848597
1101 1101 a, g dbSNP:767591426
1115 1115 c, t dbSNP:750661825
1116 1116 c, t dbSNP:61730282
1117 1117 g, t dbSNP:190302733
1118 1118 c, t dbSNP:752484290
1119 1119 a, c, g dbSNP:758417980
1125 1125 c, t dbSNP:746900412
1132 1132 a, c dbSNP:770380653
1142 1142 a, g dbSNP:780800634
1146 1146 a, c dbSNP:760755800
1149 1149 a, g dbSNP:766377918
1160 1160 c, t dbSNP:776751131
1166 1166 a, g dbSNP:764891073
1179 1179 a, g dbSNP:763843987
1189 1189 a, c dbSNP:751339103
1191 1191 c, g dbSNP:146109202
1195 1195 c, t dbSNP:767553992
1198 1198 c, g dbSNP:750330152
1200 1200 c, t dbSNP:755692915
1204 1204 a, g dbSNP:779659218
1207 1207 a, g dbSNP:748939569
1213 1213 c, t dbSNP:754644643
1217 1217 a, g dbSNP:555522870
1222 1222 c, t dbSNP:752278002
1230 1230 c, t dbSNP:201607803
1231 1231 a, g dbSNP:41298838
1232 1232 c, g dbSNP:747565599
1233 1233 a, c, t dbSNP:558072690
1236 1236 a, g dbSNP:41298840
1237 1237 c, t dbSNP:745951558
1241 1241 c, g dbSNP:769708851
1250 1250 c, g dbSNP:775788782
1252 1252 c, g dbSNP:763231000
1254 1254 c, t dbSNP:772039142
1261 1261 c, g, t dbSNP:773107514
1263 1263 c, t dbSNP:766324089
1268 1268 g, t dbSNP:753660073
1289 1289 c, g dbSNP:759090563
1296 1296 c, t dbSNP:764610884
1322 1322 a, t dbSNP:751917634
1328 1328 a, g dbSNP:549715785
1352 1352 a, g dbSNP:781731042
1361 1361 c, g dbSNP:569581176
1362 1362 a, g dbSNP:13054377
1403 1403 a, g dbSNP:548714133
1435 1435 a, g dbSNP:565927787
1443 1443 c, g dbSNP:111605258
1482 1482 a, c dbSNP:780256549
1484 1484 a, g dbSNP:749558577
1486 1486 a, c dbSNP:768991190
1487 1487 a, c dbSNP:773195263
1490 1490 a, c, t dbSNP:746812421
1491 1491 g, t dbSNP:776659796
1492 1492 a, c dbSNP:72646967
1500 1500 a, g dbSNP:764852265
1501 1501 c, g dbSNP:774951964
1503 1503 a, g dbSNP:762662497
1514 1514 c, g, t dbSNP:557935727
1516 1516 c, t dbSNP:757648761
1518 1518 c, t dbSNP:200135498
1519 1519 a, g dbSNP:750966464
1520 1520 a, g dbSNP:756543718
1521 1521 c, g, t dbSNP:780344405
1524 1524 a, g dbSNP:755047093
1526 1526 c, g, t dbSNP:779281442
1527 1527 a, g dbSNP:770781746
1528 1528 c, g dbSNP:776268518
1529 1529 a, c, g dbSNP:745858331
1531 1531 a, c dbSNP:775040109
1532 1532 c, t dbSNP:762467149
1536 1536 a, g dbSNP:763530229
1541 1541 a, g dbSNP:774028490
1545 1545 c, g dbSNP:761327170
1547 1547 c, g dbSNP:767961877
1552 1552 a, c dbSNP:571884633
1557 1557 c, t dbSNP:756699313
1558 1558 c, t dbSNP:368614435
1562 1562 c, t dbSNP:754328637
1563 1563 a, c, t dbSNP:371557427
1564 1564 a, g dbSNP:779087360
1568 1568 c, t dbSNP:753011413
1573 1573 a, g dbSNP:758517884
1575 1575 a, c dbSNP:781108085
1577 1577 a, g dbSNP:569976109
1582 1582 c, t dbSNP:769522876
1590 1590 c, t dbSNP:574947516
1591 1591 a, g dbSNP:749185545
1598 1598 a, g dbSNP:768181576
1600 1600 c, g dbSNP:773715388
1603 1603 c, g dbSNP:761590228
1604 1604 c, t dbSNP:771606854
1605 1605 a, g, t dbSNP:772860144
1612 1612 c, t dbSNP:201993443
1617 1617 c, g dbSNP:754416536
1619 1619 c, g dbSNP:760098496
1620 1620 a, c, g, t dbSNP:573980571
1621 1621 a, g dbSNP:777873225
1624 1624 c, g dbSNP:751740430
1628 1628 c, g dbSNP:755937050
1633 1633 c, t dbSNP:779779535
1642 1642 c, t dbSNP:749275474
1647 1647 a, g dbSNP:768572721
1648 1648 c, t dbSNP:778539160
1650 1650 c, g dbSNP:747623105
1653 1653 c, g dbSNP:771540171
1656 1656 c, g, t dbSNP:367711718
1659 1659 -, cac dbSNP:767638460
1660 1660 -, caccac dbSNP:753713234
1660 1660 -, cac dbSNP:761822829
1665 1665 c, t dbSNP:771471094
1668 1668 c, t dbSNP:777103863
1674 1674 c, t dbSNP:760188566
1675 1675 c, t dbSNP:765827356
1677 1677 c, g, t dbSNP:753192876
1678 1678 c, g dbSNP:764197422
1684 1684 -, ccagccgcc dbSNP:759569338
1685 1685 -, cagccgccg dbSNP:758290031
1686 1686 a, g dbSNP:553461195
1687 1687 -, gccgccgcg dbSNP:777514486
1689 1689 c, t dbSNP:573376280
1692 1692 a, c dbSNP:751755076
1695 1695 -, gcc dbSNP:756680878
1696 1696 -, gccgccgcc dbSNP:769022249
1696 1696 -, gcc dbSNP:780453322
1697 1697 c, t dbSNP:757452865
1699 1699 a, g dbSNP:766206180
1700 1700 c, t dbSNP:753613632
1701 1701 c, t dbSNP:754705790
1702 1702 -, gccgccgct dbSNP:774489872
1704 1704 c, t dbSNP:72646968
1705 1705 a, g dbSNP:778718533
1707 1707 -, gctgccgca dbSNP:748434309
1707 1707 a, c dbSNP:748075946
1708 1708 -, gctgccgca dbSNP:772030062
1709 1709 c, t dbSNP:757867291
1716 1716 -, gctgccgcg dbSNP:760701410
1716 1716 a, c dbSNP:777313046
1718 1718 a, c dbSNP:746699675
1722 1722 c, t dbSNP:770702512
1726 1726 -, gcc dbSNP:765173550
1737 1737 c, g dbSNP:371506174
1738 1738 c, t dbSNP:746250147
1745 1745 c, t dbSNP:770129913
1748 1748 c, t dbSNP:373987708
1749 1749 c, t dbSNP:775895785
1750 1750 a, g dbSNP:541198585
1751 1751 a, g dbSNP:764132783
1756 1756 a, c, g dbSNP:774386479
1760 1760 a, c, t dbSNP:762076391
1761 1761 g, t dbSNP:750574356
1764 1764 c, g, t dbSNP:754795532
1769 1769 c, t dbSNP:564141057
1770 1770 c, t dbSNP:758325399
1776 1776 c, g dbSNP:777213879
1783 1783 c, t dbSNP:746505468
1784 1784 c, g dbSNP:756769832
1786 1786 a, g dbSNP:780998431
1789 1789 a, t dbSNP:745479292
1795 1795 a, g dbSNP:770080000
1798 1798 c, t dbSNP:775903697
1800 1800 c, t dbSNP:368172043
1804 1804 c, t dbSNP:543378212
1806 1806 c, t dbSNP:774778199
1807 1807 a, g dbSNP:761882175
1810 1810 -, c dbSNP:775581777
1812 1812 c, t dbSNP:371856634
1813 1813 g, t dbSNP:773442513
1814 1814 c, t dbSNP:760927067
1817 1817 c, g dbSNP:764978950
1818 1818 a, g dbSNP:375239385
1824 1824 a, g dbSNP:563290139
1829 1829 g, t dbSNP:752531044
1832 1832 c, t dbSNP:762856074
1833 1833 c, t dbSNP:113153036
1840 1840 c, t dbSNP:751476947
1843 1843 c, t dbSNP:756861421
1883 1883 a, g dbSNP:373863680
1887 1887 c, t dbSNP:528896157
1909 1909 a, g dbSNP:72646969
1943 1943 c, g dbSNP:558979141
1955 1955 -, g dbSNP:41298842
1965 1965 c, t dbSNP:528197589
1985 1985 g, t dbSNP:551528783
1994 1994 a, g dbSNP:571783270
2053 2053 c, t dbSNP:772661629
2055 2055 a, t dbSNP:537729175
2069 2069 a, c dbSNP:551038678
2117 2117 -, g dbSNP:544636729
2121 2121 c, t dbSNP:567642855
2126 2126 g, t dbSNP:72646970
2192 2192 a, g dbSNP:72646971
2194 2194 a, t dbSNP:749619227
2208 2208 a, g dbSNP:769933350
2215 2215 c, t dbSNP:553424250
2218 2218 a, c dbSNP:72646972
2225 2225 a, g dbSNP:779040930

Target ORF information:

RefSeq Version XM_006724312
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens T-box 1 (TBX1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_006724312

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCACTTCA GCACCGTCAC CAGGGACATG GAAGCCTTCA CGGCCAGCAG CCTGAGCAGC 
CTGGGGGCCG CGGGGGGCTT CCCGGGCGCC GCGTCGCCCG GCGCCGACCC GTACGGCCCG
CGCGAGCCCC CGCCGCCGCC GCCGCGCTAC GACCCGTGCG CCGCCGCCGC CCCCGGCGCC
CCGGGCCCGC CGCCGCCGCC GCACGCCTAC CCGTTTGCGC CGGCCGCCGG GGCCGCCACC
AGCGCCGCCG CCGAGCCCGA GGGCCCCGGG GCCAGCTGCG CGGCCGCAGC CAAGGCGCCG
GTGAAGAAGA ACGCGAAGGT GGCCGGTGTG AGCGTGCAGC TAGAGATGAA GGCGCTGTGG
GACGAGTTCA ACCAGCTGGG CACCGAGATG ATCGTCACCA AGGCCGGCAG GCGGATGTTT
CCCACCTTCC AAGTGAAGCT CTTCGGCATG GATCCCATGG CCGACTATAT GCTGCTCATG
GACTTCGTGC CGGTGGACGA TAAGCGCTAC CGGTACGCCT TCCACAGCTC CTCCTGGCTG
GTGGCGGGGA AGGCCGACCC TGCCACGCCA GGCCGCGTGC ACTACCACCC GGACTCGCCT
GCCAAGGGCG CGCAGTGGAT GAAGCAAATC GTGTCCTTCG ACAAGCTCAA GCTGACCAAC
AACCTACTGG ACGACAACGG CCACATTATT CTGAATTCCA TGCACAGATA CCAGCCCCGC
TTCCACGTGG TCTATGTGGA CCCACGCAAA GATAGCGAGA AATATGCCGA GGAGAACTTC
AAAACCTTTG TGTTCGAGGA GACACGATTC ACCGCGGTCA CTGCCTACCA GAACCATCGG
ATCACGCAGC TCAAGATTGC CAGCAATCCC TTCGCGAAAG GCTTCCGGGA CTGTGACCCT
GAGGACTGGC CCCGGAACCA CCGGCCCGGC GCACTGCCGC TCATGAGCGC CTTCGCGCGC
TCGCGGAACC CCGTGGCTTC CCCGACGCAG CCCAGCGGCA CGGAGAAAGA CGCGGCTGAG
GCCCGGCGAG AATTCCAGCG CGACGCGGGC GGGCCAGCAG TGCTCGGGGA CCCGGCGCAT
CCTCCGCAGC TGCTGGCCCG GGTGCTAAGC CCCTCGCTGC CCGGGGCCGG CGGCGCCGGC
GGCTTAGTCC CGCTGCCCGG CGCGCCCGGA GGCCGGCCCA GTCCCCCGAA CCCCGAGCTG
CGCCTGGAGG CGCCCGGCGC ATCGGAGCCG CTGCACCACC ACCCCTACAA ATATCCGGCC
GCCGCCTACG ACCACTATCT CGGGGCCAAG AGCCGGCCGG CGCCCTACCC GCTGCCCGGC
CTGCGTGGCC ACGGCTACCA CCCGCACGCG CATCCGCACC ACCACCACCA CCCCGTGAGT
CCAGCCGCCG CGGCCGCCGC CGCCGCTGCC GCAGCTGCCG CGGCCGCCAA CATGTACTCG
TCGGCCGGAG CCGCGCCGCC CGGCTCCTAC GACTATTGCC CCAGATAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.