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TBX5 T-box 5 [Homo sapiens (human)]

Gene Symbol TBX5
Entrez Gene ID 6910
Full Name T-box 5
Synonyms HOS
General protein information
Preferred Names
T-box transcription factor TBX5
Names
T-box transcription factor TBX5
T-box protein 5
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12q24.1

Summary This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

601620

Disorder Html: Holt-Oram syndrome, 142900 (3)





The following TBX5 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TBX5 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu20722 NM_000192 Homo sapiens T-box 5 (TBX5), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$439
OHu21706 NM_080717 Homo sapiens T-box 5 (TBX5), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$389
OHu20722 NM_181486 Homo sapiens T-box 5 (TBX5), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$379

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu20722
Accession Version NM_000192.3
Sequence Information ORF Nucleotide Sequence (Length: 1557bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product T-box transcription factor TBX5 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF221714.1, DA863182.1, AC009260.13 and BC027942.1. This sequence is a reference standard in the RefSeqGene project. On Jun 12, 2003 this sequence version replaced gi:18201891. Summary: This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 4 both encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF221714.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGACGAGGG CTTTGGCCTG GCGCACACGC CTCTGGAGCC TGACGCAAAA 
GACCTGCCCT GCGATTCGAA ACCCGAGAGC GCGCTCGGGG CCCCCAGCAA GTCCCCGTCG
TCCCCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAGGGAA TCAAAGTGTT TCTCCATGAA
AGAGAACTGT GGCTAAAATT CCACGAAGTG GGCACGGAAA TGATCATAAC CAAGGCTGGA
AGGCGGATGT TTCCCAGTTA CAAAGTGAAG GTGACGGGCC TTAATCCCAA AACGAAGTAC
ATTCTTCTCA TGGACATTGT ACCTGCCGAC GATCACAGAT ACAAATTCGC AGATAATAAA
TGGTCTGTGA CGGGCAAAGC TGAGCCCGCC ATGCCTGGCC GCCTGTACGT GCACCCAGAC
TCCCCCGCCA CCGGGGCGCA TTGGATGAGG CAGCTCGTCT CCTTCCAGAA ACTCAAGCTC
ACCAACAACC ACCTGGACCC ATTTGGGCAT ATTATTCTAA ATTCCATGCA CAAATACCAG
CCTAGATTAC ACATCGTGAA AGCGGATGAA AATAATGGAT TTGGCTCAAA AAATACAGCG
TTCTGCACTC ACGTCTTTCC TGAGACTGCG TTTATAGCAG TGACTTCCTA CCAGAACCAC
AAGATCACGC AATTAAAGAT TGAGAATAAT CCCTTTGCCA AAGGATTTCG GGGCAGTGAT
GACATGGAGC TGCACAGAAT GTCAAGAATG CAAAGTAAAG AATATCCCGT GGTCCCCAGG
AGCACCGTGA GGCAAAAAGT GGCCTCCAAC CACAGTCCTT TCAGCAGCGA GTCTCGAGCT
CTCTCCACCT CATCCAATTT GGGGTCCCAA TACCAGTGTG AGAATGGTGT TTCCGGCCCC
TCCCAGGACC TCCTGCCTCC ACCCAACCCA TACCCACTGC CCCAGGAGCA TAGCCAAATT
TACCATTGTA CCAAGAGGAA AGAGGAAGAA TGTTCCACCA CAGACCATCC CTATAAGAAG
CCCTACATGG AGACATCACC CAGTGAAGAA GATTCCTTCT ACCGCTCTAG CTATCCACAG
CAGCAGGGCC TGGGTGCCTC CTACAGGACA GAGTCGGCAC AGCGGCAAGC TTGCATGTAT
GCCAGCTCTG CGCCCCCCAG CGAGCCTGTG CCCAGCCTAG AGGACATCAG CTGCAACACG
TGGCCAAGCA TGCCTTCCTA CAGCAGCTGC ACCGTCACCA CCGTGCAGCC CATGGACAGG
CTACCCTACC AGCACTTCTC CGCTCACTTC ACCTCGGGGC CCCTGGTCCC TCGGCTGGCT
GGCATGGCCA ACCATGGCTC CCCACAGCTG GGAGAGGGAA TGTTCCAGCA CCAGACCTCC
GTGGCCCACC AGCCTGTGGT CAGGCAGTGT GGGCCTCAGA CTGGCCTGCA GTCCCCTGGC
ACCCTTCAGC CCCCTGAGTT CCTCTACTCT CATGGCGTGC CAAGGACTCT ATCCCCTCAT
CAGTACCACT CTGTGCACGG AGTTGGCATG GTGCCAGAGT GGAGCGACAA TAGCTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000183.2
CDS668..2224
Misc Feature(1)440..442(+)
Misc Feature(2)824..1390(+)
Misc Feature(3)890..1381(+)
Misc Feature(4)962..1231(+)
Exon (1)1..629
Gene:TBX5
Gene Synonym:
Exon (2)630..814
Gene:TBX5
Gene Synonym:
Exon (3)815..909
Gene:TBX5
Gene Synonym:
Exon (4)910..1029
Gene:TBX5
Gene Synonym:
Exon (5)1030..1177
Gene:TBX5
Gene Synonym:
Exon (6)1178..1330
Gene:TBX5
Gene Synonym:
Exon (7)1331..1422
Gene:TBX5
Gene Synonym:
Exon (8)1423..1649
Gene:TBX5
Gene Synonym:
Exon (9)1650..3826
Gene:TBX5
Gene Synonym:
Translation
Position Chain Variation Link
4 4 a, g dbSNP:186960328
20 20 c, g dbSNP:79465718
40 40 c, t dbSNP:12372585
46 46 c, t dbSNP:562444249
82 82 c, g dbSNP:76464487
100 100 c, t dbSNP:544619358
123 123 c, t dbSNP:115599823
147 147 a, c dbSNP:376347706
148 148 c, t dbSNP:559798206
158 158 c, t dbSNP:769092616
178 178 c, t dbSNP:182422703
186 186 a, g dbSNP:368294179
187 187 a, c, t dbSNP:575879858
212 212 c, t dbSNP:772427559
219 219 c, g dbSNP:148864662
221 221 a, c dbSNP:769059943
224 224 a, g dbSNP:555940581
291 291 c, t dbSNP:372524573
313 313 g, t dbSNP:368923776
328 328 g, t dbSNP:748787266
339 339 a, c dbSNP:779465476
353 353 a, g dbSNP:558915347
363 363 a, c dbSNP:78462880
379 379 c, t dbSNP:756754574
380 380 c, g dbSNP:191773087
385 385 c, t dbSNP:533921054
413 413 g, t dbSNP:186472610
448 448 c, t dbSNP:111233014
501 501 a, g dbSNP:146645348
597 597 c, t dbSNP:750120924
600 600 a, c dbSNP:777744983
602 602 c, t dbSNP:756068967
633 633 a, g dbSNP:763178387
642 642 a, c, g dbSNP:769895888
643 643 g, t dbSNP:748222016
644 644 a, c, t dbSNP:769107196
645 645 c, g, t dbSNP:571328934
647 647 -, g dbSNP:750305087
649 649 -, acaggg dbSNP:762161797
653 653 a, g dbSNP:756902625
655 655 c, t dbSNP:550201404
656 656 c, t dbSNP:777370158
663 663 g, t dbSNP:755664220
673 673 c, t dbSNP:752191065
676 676 c, g dbSNP:774750288
682 682 c, t dbSNP:767487291
683 683 a, g dbSNP:145365553
687 687 a, g dbSNP:751312844
690 690 c, t dbSNP:766170094
705 705 a, c dbSNP:763125466
707 707 a, c dbSNP:773397553
718 718 c, t dbSNP:765479608
722 722 g, t dbSNP:200461617
724 724 a, c, g dbSNP:567785340
735 735 a, c, t dbSNP:141609745
738 738 g, t dbSNP:772587337
739 739 c, t dbSNP:746284842
740 740 g, t dbSNP:148195311
750 750 c, t dbSNP:755790672
754 754 a, g dbSNP:527914495
760 760 g, t dbSNP:780584178
764 764 a, g, t dbSNP:751532982
766 766 a, g dbSNP:372557517
767 767 a, g, t dbSNP:368311885
768 768 a, c, t dbSNP:761952835
771 771 a, c dbSNP:776705102
781 781 c, g, t dbSNP:34014008
782 782 c, t dbSNP:145703644
784 784 a, g dbSNP:149030937
792 792 c, t dbSNP:759976245
808 808 a, c, g, t dbSNP:564730479
811 811 a, g dbSNP:780717533
812 812 a, c dbSNP:104894383
817 817 c, t dbSNP:768418027
820 820 a, g dbSNP:746504712
823 823 g, t dbSNP:771883815
824 824 a, g dbSNP:779550194
825 825 a, g dbSNP:369919453
827 827 a, g dbSNP:145890934
828 828 c, t dbSNP:104894384
835 835 a, g dbSNP:745834660
836 836 a, t dbSNP:562706670
844 844 c, t dbSNP:544138286
850 850 a, c, g dbSNP:777853147
851 851 a, g dbSNP:756464860
868 868 c, t dbSNP:752768742
871 871 c, t dbSNP:375854421
872 872 g, t dbSNP:104894377
874 874 a, c dbSNP:760082444
883 883 a, g dbSNP:752033521
884 884 -, a dbSNP:35857654
901 901 a, g dbSNP:766962105
903 903 c, t dbSNP:763219727
905 905 a, g dbSNP:104894381
928 928 c, t dbSNP:767197919
931 931 a, g dbSNP:758964536
932 932 a, g dbSNP:778885169
939 939 c, t dbSNP:775593443
943 943 c, g dbSNP:773971213
944 944 a, g dbSNP:770717773
956 956 a, g dbSNP:749255660
960 960 a, c dbSNP:772844823
961 961 a, g dbSNP:769736865
968 968 a, t dbSNP:515726234
974 974 c, g dbSNP:748009824
976 976 c, t dbSNP:28730763
982 982 c, t dbSNP:745985270
983 983 a, g dbSNP:147710408
985 985 c, t dbSNP:141457646
989 989 a, c, t dbSNP:376621016
994 994 c, t dbSNP:749667596
998 998 g, t dbSNP:77357563
1005 1005 a, g dbSNP:483353129
1007 1007 c, t dbSNP:757586102
1017 1017 c, t dbSNP:754267671
1021 1021 c, t dbSNP:151249768
1023 1023 a, g dbSNP:556671717
1026 1026 a, g dbSNP:750969565
1034 1034 a, g dbSNP:754145043
1039 1039 a, g dbSNP:778117002
1042 1042 c, t dbSNP:142293206
1057 1057 c, t dbSNP:751249347
1075 1075 a, c dbSNP:104894379
1076 1076 a, g dbSNP:765999767
1087 1087 c, g dbSNP:148715804
1094 1094 a, g dbSNP:374906778
1099 1099 a, c, t dbSNP:146038442
1102 1102 a, g dbSNP:761842850
1105 1105 c, g dbSNP:776523515
1107 1107 a, g dbSNP:768515930
1117 1117 g, t dbSNP:760434419
1123 1123 c, t dbSNP:775748269
1124 1124 a, g dbSNP:551461950
1125 1125 a, t dbSNP:772248871
1129 1129 a, c dbSNP:745934847
1132 1132 c, t dbSNP:778977800
1134 1134 a, t dbSNP:771361403
1138 1138 a, g dbSNP:749827599
1147 1147 c, t dbSNP:778064192
1150 1150 c, g dbSNP:756481812
1170 1170 c, t dbSNP:753047224
1207 1207 a, g dbSNP:770274405
1222 1222 c, t dbSNP:142631741
1223 1223 a, g dbSNP:557183241
1230 1230 c, t dbSNP:758079210
1231 1231 a, g, t dbSNP:745378130
1243 1243 c, t dbSNP:201212120
1251 1251 c, g dbSNP:564042302
1252 1252 c, t dbSNP:753457483
1266 1266 a, c dbSNP:370771953
1267 1267 a, g, t dbSNP:139329918
1270 1270 c, t dbSNP:767238220
1271 1271 c, t dbSNP:759713411
1273 1273 c, t dbSNP:774522949
1276 1276 c, t dbSNP:146230255
1277 1277 c, g dbSNP:763004247
1279 1279 c, t dbSNP:200326827
1280 1280 a, g, t dbSNP:190881877
1282 1282 c, g dbSNP:200921127
1285 1285 c, g, t dbSNP:533581420
1291 1291 c, g dbSNP:769113870
1294 1294 g, t dbSNP:747429505
1306 1306 a, g dbSNP:149274578
1318 1318 c, t dbSNP:201394336
1324 1324 c, t dbSNP:778217968
1328 1328 a, g dbSNP:757039207
1330 1330 c, g dbSNP:748766700
1336 1336 a, g dbSNP:376519728
1340 1340 c, t dbSNP:765547666
1363 1363 c, t dbSNP:747652988
1376 1376 c, t dbSNP:104894382
1377 1377 a, g dbSNP:104894378
1378 1378 a, g dbSNP:754018766
1381 1381 c, t dbSNP:764630371
1382 1382 a, g dbSNP:761278614
1390 1390 c, t dbSNP:368563052
1391 1391 a, g dbSNP:772595679
1394 1394 a, g dbSNP:759983816
1396 1396 g, t dbSNP:186183947
1399 1399 g, t dbSNP:769541948
1403 1403 a, c, g dbSNP:374600913
1414 1414 a, g dbSNP:138552878
1435 1435 c, t dbSNP:370805715
1436 1436 a, g dbSNP:200382742
1448 1448 a, t dbSNP:377625550
1450 1450 -, cccaggagc dbSNP:766716477
1453 1453 c, t dbSNP:375955080
1454 1454 a, g dbSNP:147405081
1458 1458 a, g dbSNP:201071418
1462 1462 a, g dbSNP:778784399
1465 1465 a, t dbSNP:777287804
1466 1466 a, g dbSNP:757373962
1471 1471 c, g dbSNP:35110399
1480 1480 c, t dbSNP:778180226
1484 1484 a, c dbSNP:756238293
1485 1485 c, g dbSNP:752879569
1494 1494 a, g dbSNP:147977741
1495 1495 c, t dbSNP:146908258
1496 1496 a, g dbSNP:369697961
1501 1501 c, t dbSNP:142609815
1503 1503 a, g dbSNP:115178276
1504 1504 a, c dbSNP:564499369
1507 1507 c, t dbSNP:763797226
1508 1508 c, t dbSNP:760177359
1509 1509 g, t dbSNP:774986848
1515 1515 a, c dbSNP:147077037
1521 1521 c, t dbSNP:537547818
1522 1522 c, t dbSNP:745840026
1526 1526 c, t dbSNP:773978838
1531 1531 g, t dbSNP:770728362
1532 1532 g, t dbSNP:543015692
1534 1534 c, t dbSNP:778126616
1561 1561 c, t dbSNP:770206057
1562 1562 a, g dbSNP:201637366
1563 1563 g, t dbSNP:781342709
1567 1567 c, t dbSNP:755387654
1577 1577 c, t dbSNP:752134526
1581 1581 c, g, t dbSNP:758752836
1585 1585 a, t dbSNP:750855891
1586 1586 c, t dbSNP:763672657
1589 1589 c, t dbSNP:760334883
1594 1594 a, c dbSNP:201164281
1611 1611 a, g dbSNP:752101162
1615 1615 a, g dbSNP:767086503
1632 1632 a, g dbSNP:759009256
1641 1641 a, g dbSNP:774321607
1651 1651 a, g dbSNP:750350579
1652 1652 a, g dbSNP:142807139
1658 1658 a, t dbSNP:765327234
1662 1662 c, t dbSNP:374446377
1671 1671 a, g dbSNP:776297033
1680 1680 a, g dbSNP:764322332
1682 1682 a, g dbSNP:760830193
1687 1687 a, g dbSNP:148977955
1689 1689 c, g dbSNP:772109169
1695 1695 c, t dbSNP:745997257
1696 1696 a, g dbSNP:774825317
1699 1699 a, g dbSNP:771408470
1701 1701 a, c dbSNP:749598173
1706 1706 a, c dbSNP:778011764
1730 1730 c, t dbSNP:372827955
1731 1731 a, g, t dbSNP:145784562
1743 1743 c, t dbSNP:149792175
1749 1749 a, g dbSNP:140945366
1751 1751 c, g dbSNP:765204502
1768 1768 c, g dbSNP:763029336
1780 1780 a, g dbSNP:757158327
1781 1781 a, t dbSNP:79040021
1782 1782 c, t dbSNP:143068551
1783 1783 a, g dbSNP:763827720
1790 1790 c, t dbSNP:377532269
1792 1792 g, t dbSNP:775667911
1802 1802 a, c, t dbSNP:759607221
1804 1804 c, g dbSNP:774419025
1807 1807 c, t dbSNP:771409350
1809 1809 a, c dbSNP:763390789
1810 1810 a, c dbSNP:773582799
1818 1818 c, t dbSNP:530882236
1819 1819 a, g dbSNP:200073406
1824 1824 a, c, t dbSNP:771857554
1825 1825 c, t dbSNP:745319218
1828 1828 a, c, g, t dbSNP:753688559
1829 1829 a, g dbSNP:139371720
1838 1838 c, t dbSNP:755849744
1846 1846 a, g dbSNP:752553883
1851 1851 a, g dbSNP:146269931
1866 1866 c, t dbSNP:759766836
1867 1867 a, g dbSNP:751588696
1870 1870 g, t dbSNP:377649723
1873 1873 a, c dbSNP:763129045
1875 1875 c, g dbSNP:773568096
1882 1882 a, t dbSNP:770387292
1891 1891 a, c dbSNP:762204624
1899 1899 c, t dbSNP:267603320
1900 1900 c, g, t dbSNP:188839350
1901 1901 a, c, g dbSNP:114124210
1909 1909 c, t dbSNP:370133092
1910 1910 a, g dbSNP:748889317
1927 1927 c, g dbSNP:780330016
1930 1930 a, g dbSNP:777568811
1936 1936 c, t dbSNP:756049331
1937 1937 a, c, g dbSNP:780987688
1940 1940 a, c dbSNP:755152246
1945 1945 c, t dbSNP:751745926
1948 1948 c, t dbSNP:6489957
1949 1949 a, g, t dbSNP:577615373
1960 1960 c, g dbSNP:143971376
1963 1963 a, g dbSNP:149474574
1966 1966 g, t dbSNP:777099745
1972 1972 g, t dbSNP:372185479
1979 1979 c, t dbSNP:761170474
1980 1980 a, g dbSNP:557758851
1986 1986 c, t dbSNP:770685602
2008 2008 c, g dbSNP:748678010
2010 2010 c, t dbSNP:772790458
2011 2011 a, g dbSNP:769669494
2018 2018 a, g dbSNP:200704209
2025 2025 a, g dbSNP:781143449
2042 2042 a, g dbSNP:754740593
2047 2047 c, t dbSNP:575162861
2048 2048 a, g dbSNP:201152128
2051 2051 a, g dbSNP:758663811
2055 2055 a, c dbSNP:750423209
2063 2063 a, g dbSNP:765443283
2067 2067 c, t dbSNP:757457183
2074 2074 c, g dbSNP:373007088
2080 2080 a, g dbSNP:764756835
2082 2082 c, t dbSNP:761119136
2086 2086 a, g dbSNP:1048078
2092 2092 c, g, t dbSNP:199729532
2095 2095 a, g dbSNP:762687473
2109 2109 c, t dbSNP:772556634
2110 2110 c, t dbSNP:137928706
2116 2116 a, g dbSNP:117965596
2118 2118 a, c, t dbSNP:768644203
2120 2120 c, g dbSNP:746726123
2130 2130 c, t dbSNP:779923573
2143 2143 a, c, t dbSNP:369034176
2144 2144 a, g dbSNP:556197042
2153 2153 a, g dbSNP:757399952
2157 2157 c, t dbSNP:374676021
2158 2158 a, g dbSNP:778359709
2162 2162 c, g dbSNP:756656267
2173 2173 c, t dbSNP:752953396
2185 2185 c, t dbSNP:767955877
2186 2186 a, g dbSNP:374924325
2190 2190 c, t dbSNP:147339942
2196 2196 a, t dbSNP:764923882
2212 2212 a, c, t dbSNP:186780790
2213 2213 a, g dbSNP:200354838
2221 2221 c, t dbSNP:530920048
2223 2223 a, g dbSNP:775333759
2227 2227 c, t dbSNP:771881586
2228 2228 g, t dbSNP:745708633
2230 2230 a, g dbSNP:779273607
2236 2236 c, t dbSNP:201497094
2243 2243 a, g dbSNP:749324850
2244 2244 -, ca dbSNP:763422136
2253 2253 c, t dbSNP:201997498
2257 2257 a, g dbSNP:756537403
2261 2261 -, aagaga dbSNP:112808902
2261 2261 -, aa dbSNP:773782075
2264 2264 a, g dbSNP:10850326
2267 2267 g, t dbSNP:371119204
2269 2269 a, g dbSNP:377033399
2274 2274 -, ag dbSNP:201961625
2275 2275 -, ag, agag dbSNP:762460052
2288 2288 -, ga dbSNP:768653470
2289 2289 -, aag dbSNP:747209673
2294 2294 a, g dbSNP:541667882
2301 2301 a, g dbSNP:28730761
2302 2302 g, t dbSNP:764799644
2306 2306 a, c dbSNP:761479218
2312 2312 c, g dbSNP:753308282
2314 2314 a, t dbSNP:763633224
2317 2317 c, t dbSNP:76633287
2318 2318 c, g dbSNP:76347803
2319 2319 a, c dbSNP:78828439
2320 2320 -, cg dbSNP:780156117
2320 2320 a, c dbSNP:76694710
2321 2321 a, g dbSNP:883079
2327 2327 c, g dbSNP:543786091
2329 2329 c, t dbSNP:771970874
2345 2345 a, g dbSNP:112051831
2353 2353 a, c dbSNP:28730760
2354 2354 a, t dbSNP:371880710
2359 2359 a, t dbSNP:771227659
2363 2363 a, g dbSNP:749552000
2368 2368 c, t dbSNP:535494778
2403 2403 c, g dbSNP:181130921
2410 2410 a, g dbSNP:769894729
2492 2492 c, t dbSNP:2384410
2514 2514 a, g dbSNP:370109574
2519 2519 c, t dbSNP:552724647
2530 2530 c, t dbSNP:571901755
2577 2577 a, g dbSNP:745450726
2608 2608 c, t dbSNP:12426660
2631 2631 a, g dbSNP:757238614
2650 2650 g, t dbSNP:552425924
2701 2701 -, t dbSNP:151183753
2703 2703 a, g dbSNP:536993995
2704 2704 a, g dbSNP:569991822
2720 2720 a, g dbSNP:552017501
2741 2741 c, t dbSNP:773913802
2745 2745 a, g dbSNP:758948929
2748 2748 a, t dbSNP:76799455
2751 2751 c, g dbSNP:566193674
2787 2787 a, g dbSNP:78058633
2796 2796 a, t dbSNP:189764599
2825 2825 a, g dbSNP:116382074
2887 2887 a, c dbSNP:559194135
2893 2893 c, t dbSNP:766553397
2897 2897 c, t dbSNP:140532076
2906 2906 c, g dbSNP:531923731
2907 2907 c, t dbSNP:563250446
2920 2920 a, g dbSNP:541449392
2926 2926 c, t dbSNP:759107305
2941 2941 c, t dbSNP:148346089
2960 2960 c, t dbSNP:552634822
2962 2962 a, c dbSNP:553621044
2963 2963 c, t dbSNP:117414057
3008 3008 a, g dbSNP:776283407
3036 3036 c, t dbSNP:143511878
3045 3045 c, t dbSNP:760503694
3060 3060 a, c dbSNP:537303672
3066 3066 c, t dbSNP:569872023
3111 3111 a, g dbSNP:571557978
3124 3124 c, t dbSNP:773180889
3133 3133 a, g dbSNP:184360838
3168 3168 a, t dbSNP:536633001
3184 3184 a, g dbSNP:566107581
3189 3189 g, t dbSNP:547907580
3195 3195 c, g dbSNP:777236533
3243 3243 c, t dbSNP:764387876
3287 3287 c, t dbSNP:192445542
3290 3290 c, t dbSNP:188337192
3309 3309 c, t dbSNP:549280585
3318 3318 a, c dbSNP:184793497
3325 3325 a, g dbSNP:6489956
3412 3412 c, t dbSNP:141223729
3422 3422 a, g dbSNP:557604192
3435 3435 a, g dbSNP:532005395
3477 3477 c, t dbSNP:561439634
3536 3536 c, t dbSNP:542952088
3542 3542 -, gttttttt dbSNP:776263824
3551 3551 -, t dbSNP:201799304
3552 3552 -, t dbSNP:373833293
3555 3555 g, t dbSNP:535072534
3559 3559 -, t dbSNP:201166554
3581 3581 c, t dbSNP:147774482
3597 3597 g, t dbSNP:192527148
3599 3599 -, a dbSNP:199773239
3600 3600 a, t dbSNP:75334514
3609 3609 c, t dbSNP:200407853
3609 3609 -, t dbSNP:35534655
3611 3611 c, t dbSNP:202095845
3670 3670 g, t dbSNP:576867150
3691 3691 c, g dbSNP:558688524
3701 3701 -, aagt dbSNP:564562667
3719 3719 c, g dbSNP:187379531
3744 3744 g, t dbSNP:183595902
3755 3755 -, g dbSNP:34050413
3805 3805 a, g dbSNP:747205317

Target ORF information:

RefSeq Version NM_000192
Organism Homo sapiens (human)
Definition Homo sapiens T-box 5 (TBX5), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000192

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGACGAGGG CTTTGGCCTG GCGCACACGC CTCTGGAGCC TGACGCAAAA 
GACCTGCCCT GCGATTCGAA ACCCGAGAGC GCGCTCGGGG CCCCCAGCAA GTCCCCGTCG
TCCCCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAGGGAA TCAAAGTGTT TCTCCATGAA
AGAGAACTGT GGCTAAAATT CCACGAAGTG GGCACGGAAA TGATCATAAC CAAGGCTGGA
AGGCGGATGT TTCCCAGTTA CAAAGTGAAG GTGACGGGCC TTAATCCCAA AACGAAGTAC
ATTCTTCTCA TGGACATTGT ACCTGCCGAC GATCACAGAT ACAAATTCGC AGATAATAAA
TGGTCTGTGA CGGGCAAAGC TGAGCCCGCC ATGCCTGGCC GCCTGTACGT GCACCCAGAC
TCCCCCGCCA CCGGGGCGCA TTGGATGAGG CAGCTCGTCT CCTTCCAGAA ACTCAAGCTC
ACCAACAACC ACCTGGACCC ATTTGGGCAT ATTATTCTAA ATTCCATGCA CAAATACCAG
CCTAGATTAC ACATCGTGAA AGCGGATGAA AATAATGGAT TTGGCTCAAA AAATACAGCG
TTCTGCACTC ACGTCTTTCC TGAGACTGCG TTTATAGCAG TGACTTCCTA CCAGAACCAC
AAGATCACGC AATTAAAGAT TGAGAATAAT CCCTTTGCCA AAGGATTTCG GGGCAGTGAT
GACATGGAGC TGCACAGAAT GTCAAGAATG CAAAGTAAAG AATATCCCGT GGTCCCCAGG
AGCACCGTGA GGCAAAAAGT GGCCTCCAAC CACAGTCCTT TCAGCAGCGA GTCTCGAGCT
CTCTCCACCT CATCCAATTT GGGGTCCCAA TACCAGTGTG AGAATGGTGT TTCCGGCCCC
TCCCAGGACC TCCTGCCTCC ACCCAACCCA TACCCACTGC CCCAGGAGCA TAGCCAAATT
TACCATTGTA CCAAGAGGAA AGAGGAAGAA TGTTCCACCA CAGACCATCC CTATAAGAAG
CCCTACATGG AGACATCACC CAGTGAAGAA GATTCCTTCT ACCGCTCTAG CTATCCACAG
CAGCAGGGCC TGGGTGCCTC CTACAGGACA GAGTCGGCAC AGCGGCAAGC TTGCATGTAT
GCCAGCTCTG CGCCCCCCAG CGAGCCTGTG CCCAGCCTAG AGGACATCAG CTGCAACACG
TGGCCAAGCA TGCCTTCCTA CAGCAGCTGC ACCGTCACCA CCGTGCAGCC CATGGACAGG
CTACCCTACC AGCACTTCTC CGCTCACTTC ACCTCGGGGC CCCTGGTCCC TCGGCTGGCT
GGCATGGCCA ACCATGGCTC CCCACAGCTG GGAGAGGGAA TGTTCCAGCA CCAGACCTCC
GTGGCCCACC AGCCTGTGGT CAGGCAGTGT GGGCCTCAGA CTGGCCTGCA GTCCCCTGGC
ACCCTTCAGC CCCCTGAGTT CCTCTACTCT CATGGCGTGC CAAGGACTCT ATCCCCTCAT
CAGTACCACT CTGTGCACGG AGTTGGCATG GTGCCAGAGT GGAGCGACAA TAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21706
Accession Version NM_080717.2
Sequence Information ORF Nucleotide Sequence (Length: 1407bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product T-box transcription factor TBX5 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF221714.1, AB051068.1, AC009260.13 and BC027942.1. On Jun 12, 2003 this sequence version replaced gi:18201893. Summary: This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (3) lacks the exon containing the translation start site compared to transcript variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB051068.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGAGGGAA TCAAAGTGTT TCTCCATGAA AGAGAACTGT GGCTAAAATT CCACGAAGTG 
GGCACGGAAA TGATCATAAC CAAGGCTGGA AGGCGGATGT TTCCCAGTTA CAAAGTGAAG
GTGACGGGCC TTAATCCCAA AACGAAGTAC ATTCTTCTCA TGGACATTGT ACCTGCCGAC
GATCACAGAT ACAAATTCGC AGATAATAAA TGGTCTGTGA CGGGCAAAGC TGAGCCCGCC
ATGCCTGGCC GCCTGTACGT GCACCCAGAC TCCCCCGCCA CCGGGGCGCA TTGGATGAGG
CAGCTCGTCT CCTTCCAGAA ACTCAAGCTC ACCAACAACC ACCTGGACCC ATTTGGGCAT
ATTATTCTAA ATTCCATGCA CAAATACCAG CCTAGATTAC ACATCGTGAA AGCGGATGAA
AATAATGGAT TTGGCTCAAA AAATACAGCG TTCTGCACTC ACGTCTTTCC TGAGACTGCG
TTTATAGCAG TGACTTCCTA CCAGAACCAC AAGATCACGC AATTAAAGAT TGAGAATAAT
CCCTTTGCCA AAGGATTTCG GGGCAGTGAT GACATGGAGC TGCACAGAAT GTCAAGAATG
CAAAGTAAAG AATATCCCGT GGTCCCCAGG AGCACCGTGA GGCAAAAAGT GGCCTCCAAC
CACAGTCCTT TCAGCAGCGA GTCTCGAGCT CTCTCCACCT CATCCAATTT GGGGTCCCAA
TACCAGTGTG AGAATGGTGT TTCCGGCCCC TCCCAGGACC TCCTGCCTCC ACCCAACCCA
TACCCACTGC CCCAGGAGCA TAGCCAAATT TACCATTGTA CCAAGAGGAA AGAGGAAGAA
TGTTCCACCA CAGACCATCC CTATAAGAAG CCCTACATGG AGACATCACC CAGTGAAGAA
GATTCCTTCT ACCGCTCTAG CTATCCACAG CAGCAGGGCC TGGGTGCCTC CTACAGGACA
GAGTCGGCAC AGCGGCAAGC TTGCATGTAT GCCAGCTCTG CGCCCCCCAG CGAGCCTGTG
CCCAGCCTAG AGGACATCAG CTGCAACACG TGGCCAAGCA TGCCTTCCTA CAGCAGCTGC
ACCGTCACCA CCGTGCAGCC CATGGACAGG CTACCCTACC AGCACTTCTC CGCTCACTTC
ACCTCGGGGC CCCTGGTCCC TCGGCTGGCT GGCATGGCCA ACCATGGCTC CCCACAGCTG
GGAGAGGGAA TGTTCCAGCA CCAGACCTCC GTGGCCCACC AGCCTGTGGT CAGGCAGTGT
GGGCCTCAGA CTGGCCTGCA GTCCCCTGGC ACCCTTCAGC CCCCTGAGTT CCTCTACTCT
CATGGCGTGC CAAGGACTCT ATCCCCTCAT CAGTACCACT CTGTGCACGG AGTTGGCATG
GTGCCAGAGT GGAGCGACAA TAGCTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_542448.1
CDS633..2039
Misc Feature(1)621..623(+)
Misc Feature(2)639..1205(+)
Misc Feature(3)705..1196(+)
Misc Feature(4)777..1046(+)
Exon (1)1..629
Gene:TBX5
Gene Synonym:
Exon (2)630..724
Gene:TBX5
Gene Synonym:
Exon (3)725..844
Gene:TBX5
Gene Synonym:
Exon (4)845..992
Gene:TBX5
Gene Synonym:
Exon (5)993..1145
Gene:TBX5
Gene Synonym:
Exon (6)1146..1237
Gene:TBX5
Gene Synonym:
Exon (7)1238..1464
Gene:TBX5
Gene Synonym:
Exon (8)1465..3641
Gene:TBX5
Gene Synonym:
Translation
Position Chain Variation Link
4 4 a, g dbSNP:186960328
20 20 c, g dbSNP:79465718
40 40 c, t dbSNP:12372585
46 46 c, t dbSNP:562444249
82 82 c, g dbSNP:76464487
100 100 c, t dbSNP:544619358
123 123 c, t dbSNP:115599823
147 147 a, c dbSNP:376347706
148 148 c, t dbSNP:559798206
158 158 c, t dbSNP:769092616
178 178 c, t dbSNP:182422703
186 186 a, g dbSNP:368294179
187 187 a, c, t dbSNP:575879858
212 212 c, t dbSNP:772427559
219 219 c, g dbSNP:148864662
221 221 a, c dbSNP:769059943
224 224 a, g dbSNP:555940581
291 291 c, t dbSNP:372524573
313 313 g, t dbSNP:368923776
328 328 g, t dbSNP:748787266
339 339 a, c dbSNP:779465476
353 353 a, g dbSNP:558915347
363 363 a, c dbSNP:78462880
379 379 c, t dbSNP:756754574
380 380 c, g dbSNP:191773087
385 385 c, t dbSNP:533921054
413 413 g, t dbSNP:186472610
448 448 c, t dbSNP:111233014
501 501 a, g dbSNP:146645348
597 597 c, t dbSNP:750120924
600 600 a, c dbSNP:777744983
602 602 c, t dbSNP:756068967
632 632 c, t dbSNP:768418027
635 635 a, g dbSNP:746504712
638 638 g, t dbSNP:771883815
639 639 a, g dbSNP:779550194
640 640 a, g dbSNP:369919453
642 642 a, g dbSNP:145890934
643 643 c, t dbSNP:104894384
650 650 a, g dbSNP:745834660
651 651 a, t dbSNP:562706670
659 659 c, t dbSNP:544138286
665 665 a, c, g dbSNP:777853147
666 666 a, g dbSNP:756464860
683 683 c, t dbSNP:752768742
686 686 c, t dbSNP:375854421
687 687 g, t dbSNP:104894377
689 689 a, c dbSNP:760082444
698 698 a, g dbSNP:752033521
699 699 -, a dbSNP:35857654
716 716 a, g dbSNP:766962105
718 718 c, t dbSNP:763219727
720 720 a, g dbSNP:104894381
743 743 c, t dbSNP:767197919
746 746 a, g dbSNP:758964536
747 747 a, g dbSNP:778885169
754 754 c, t dbSNP:775593443
758 758 c, g dbSNP:773971213
759 759 a, g dbSNP:770717773
771 771 a, g dbSNP:749255660
775 775 a, c dbSNP:772844823
776 776 a, g dbSNP:769736865
783 783 a, t dbSNP:515726234
789 789 c, g dbSNP:748009824
791 791 c, t dbSNP:28730763
797 797 c, t dbSNP:745985270
798 798 a, g dbSNP:147710408
800 800 c, t dbSNP:141457646
804 804 a, c, t dbSNP:376621016
809 809 c, t dbSNP:749667596
813 813 g, t dbSNP:77357563
820 820 a, g dbSNP:483353129
822 822 c, t dbSNP:757586102
832 832 c, t dbSNP:754267671
836 836 c, t dbSNP:151249768
838 838 a, g dbSNP:556671717
841 841 a, g dbSNP:750969565
849 849 a, g dbSNP:754145043
854 854 a, g dbSNP:778117002
857 857 c, t dbSNP:142293206
872 872 c, t dbSNP:751249347
890 890 a, c dbSNP:104894379
891 891 a, g dbSNP:765999767
902 902 c, g dbSNP:148715804
909 909 a, g dbSNP:374906778
914 914 a, c, t dbSNP:146038442
917 917 a, g dbSNP:761842850
920 920 c, g dbSNP:776523515
922 922 a, g dbSNP:768515930
932 932 g, t dbSNP:760434419
938 938 c, t dbSNP:775748269
939 939 a, g dbSNP:551461950
940 940 a, t dbSNP:772248871
944 944 a, c dbSNP:745934847
947 947 c, t dbSNP:778977800
949 949 a, t dbSNP:771361403
953 953 a, g dbSNP:749827599
962 962 c, t dbSNP:778064192
965 965 c, g dbSNP:756481812
985 985 c, t dbSNP:753047224
1022 1022 a, g dbSNP:770274405
1037 1037 c, t dbSNP:142631741
1038 1038 a, g dbSNP:557183241
1045 1045 c, t dbSNP:758079210
1046 1046 a, g, t dbSNP:745378130
1058 1058 c, t dbSNP:201212120
1066 1066 c, g dbSNP:564042302
1067 1067 c, t dbSNP:753457483
1081 1081 a, c dbSNP:370771953
1082 1082 a, g, t dbSNP:139329918
1085 1085 c, t dbSNP:767238220
1086 1086 c, t dbSNP:759713411
1088 1088 c, t dbSNP:774522949
1091 1091 c, t dbSNP:146230255
1092 1092 c, g dbSNP:763004247
1094 1094 c, t dbSNP:200326827
1095 1095 a, g, t dbSNP:190881877
1097 1097 c, g dbSNP:200921127
1100 1100 c, g, t dbSNP:533581420
1106 1106 c, g dbSNP:769113870
1109 1109 g, t dbSNP:747429505
1121 1121 a, g dbSNP:149274578
1133 1133 c, t dbSNP:201394336
1139 1139 c, t dbSNP:778217968
1143 1143 a, g dbSNP:757039207
1145 1145 c, g dbSNP:748766700
1151 1151 a, g dbSNP:376519728
1155 1155 c, t dbSNP:765547666
1178 1178 c, t dbSNP:747652988
1191 1191 c, t dbSNP:104894382
1192 1192 a, g dbSNP:104894378
1193 1193 a, g dbSNP:754018766
1196 1196 c, t dbSNP:764630371
1197 1197 a, g dbSNP:761278614
1205 1205 c, t dbSNP:368563052
1206 1206 a, g dbSNP:772595679
1209 1209 a, g dbSNP:759983816
1211 1211 g, t dbSNP:186183947
1214 1214 g, t dbSNP:769541948
1218 1218 a, c, g dbSNP:374600913
1229 1229 a, g dbSNP:138552878
1250 1250 c, t dbSNP:370805715
1251 1251 a, g dbSNP:200382742
1263 1263 a, t dbSNP:377625550
1265 1265 -, cccaggagc dbSNP:766716477
1268 1268 c, t dbSNP:375955080
1269 1269 a, g dbSNP:147405081
1273 1273 a, g dbSNP:201071418
1277 1277 a, g dbSNP:778784399
1280 1280 a, t dbSNP:777287804
1281 1281 a, g dbSNP:757373962
1286 1286 c, g dbSNP:35110399
1295 1295 c, t dbSNP:778180226
1299 1299 a, c dbSNP:756238293
1300 1300 c, g dbSNP:752879569
1309 1309 a, g dbSNP:147977741
1310 1310 c, t dbSNP:146908258
1311 1311 a, g dbSNP:369697961
1316 1316 c, t dbSNP:142609815
1318 1318 a, g dbSNP:115178276
1319 1319 a, c dbSNP:564499369
1322 1322 c, t dbSNP:763797226
1323 1323 c, t dbSNP:760177359
1324 1324 g, t dbSNP:774986848
1330 1330 a, c dbSNP:147077037
1336 1336 c, t dbSNP:537547818
1337 1337 c, t dbSNP:745840026
1341 1341 c, t dbSNP:773978838
1346 1346 g, t dbSNP:770728362
1347 1347 g, t dbSNP:543015692
1349 1349 c, t dbSNP:778126616
1376 1376 c, t dbSNP:770206057
1377 1377 a, g dbSNP:201637366
1378 1378 g, t dbSNP:781342709
1382 1382 c, t dbSNP:755387654
1392 1392 c, t dbSNP:752134526
1396 1396 c, g, t dbSNP:758752836
1400 1400 a, t dbSNP:750855891
1401 1401 c, t dbSNP:763672657
1404 1404 c, t dbSNP:760334883
1409 1409 a, c dbSNP:201164281
1426 1426 a, g dbSNP:752101162
1430 1430 a, g dbSNP:767086503
1447 1447 a, g dbSNP:759009256
1456 1456 a, g dbSNP:774321607
1466 1466 a, g dbSNP:750350579
1467 1467 a, g dbSNP:142807139
1473 1473 a, t dbSNP:765327234
1477 1477 c, t dbSNP:374446377
1486 1486 a, g dbSNP:776297033
1495 1495 a, g dbSNP:764322332
1497 1497 a, g dbSNP:760830193
1502 1502 a, g dbSNP:148977955
1504 1504 c, g dbSNP:772109169
1510 1510 c, t dbSNP:745997257
1511 1511 a, g dbSNP:774825317
1514 1514 a, g dbSNP:771408470
1516 1516 a, c dbSNP:749598173
1521 1521 a, c dbSNP:778011764
1545 1545 c, t dbSNP:372827955
1546 1546 a, g, t dbSNP:145784562
1558 1558 c, t dbSNP:149792175
1564 1564 a, g dbSNP:140945366
1566 1566 c, g dbSNP:765204502
1583 1583 c, g dbSNP:763029336
1595 1595 a, g dbSNP:757158327
1596 1596 a, t dbSNP:79040021
1597 1597 c, t dbSNP:143068551
1598 1598 a, g dbSNP:763827720
1605 1605 c, t dbSNP:377532269
1607 1607 g, t dbSNP:775667911
1617 1617 a, c, t dbSNP:759607221
1619 1619 c, g dbSNP:774419025
1622 1622 c, t dbSNP:771409350
1624 1624 a, c dbSNP:763390789
1625 1625 a, c dbSNP:773582799
1633 1633 c, t dbSNP:530882236
1634 1634 a, g dbSNP:200073406
1639 1639 a, c, t dbSNP:771857554
1640 1640 c, t dbSNP:745319218
1643 1643 a, c, g, t dbSNP:753688559
1644 1644 a, g dbSNP:139371720
1653 1653 c, t dbSNP:755849744
1661 1661 a, g dbSNP:752553883
1666 1666 a, g dbSNP:146269931
1681 1681 c, t dbSNP:759766836
1682 1682 a, g dbSNP:751588696
1685 1685 g, t dbSNP:377649723
1688 1688 a, c dbSNP:763129045
1690 1690 c, g dbSNP:773568096
1697 1697 a, t dbSNP:770387292
1706 1706 a, c dbSNP:762204624
1714 1714 c, t dbSNP:267603320
1715 1715 c, g, t dbSNP:188839350
1716 1716 a, c, g dbSNP:114124210
1724 1724 c, t dbSNP:370133092
1725 1725 a, g dbSNP:748889317
1742 1742 c, g dbSNP:780330016
1745 1745 a, g dbSNP:777568811
1751 1751 c, t dbSNP:756049331
1752 1752 a, c, g dbSNP:780987688
1755 1755 a, c dbSNP:755152246
1760 1760 c, t dbSNP:751745926
1763 1763 c, t dbSNP:6489957
1764 1764 a, g, t dbSNP:577615373
1775 1775 c, g dbSNP:143971376
1778 1778 a, g dbSNP:149474574
1781 1781 g, t dbSNP:777099745
1787 1787 g, t dbSNP:372185479
1794 1794 c, t dbSNP:761170474
1795 1795 a, g dbSNP:557758851
1801 1801 c, t dbSNP:770685602
1823 1823 c, g dbSNP:748678010
1825 1825 c, t dbSNP:772790458
1826 1826 a, g dbSNP:769669494
1833 1833 a, g dbSNP:200704209
1840 1840 a, g dbSNP:781143449
1857 1857 a, g dbSNP:754740593
1862 1862 c, t dbSNP:575162861
1863 1863 a, g dbSNP:201152128
1866 1866 a, g dbSNP:758663811
1870 1870 a, c dbSNP:750423209
1878 1878 a, g dbSNP:765443283
1882 1882 c, t dbSNP:757457183
1889 1889 c, g dbSNP:373007088
1895 1895 a, g dbSNP:764756835
1897 1897 c, t dbSNP:761119136
1901 1901 a, g dbSNP:1048078
1907 1907 c, g, t dbSNP:199729532
1910 1910 a, g dbSNP:762687473
1924 1924 c, t dbSNP:772556634
1925 1925 c, t dbSNP:137928706
1931 1931 a, g dbSNP:117965596
1933 1933 a, c, t dbSNP:768644203
1935 1935 c, g dbSNP:746726123
1945 1945 c, t dbSNP:779923573
1958 1958 a, c, t dbSNP:369034176
1959 1959 a, g dbSNP:556197042
1968 1968 a, g dbSNP:757399952
1972 1972 c, t dbSNP:374676021
1973 1973 a, g dbSNP:778359709
1977 1977 c, g dbSNP:756656267
1988 1988 c, t dbSNP:752953396
2000 2000 c, t dbSNP:767955877
2001 2001 a, g dbSNP:374924325
2005 2005 c, t dbSNP:147339942
2011 2011 a, t dbSNP:764923882
2027 2027 a, c, t dbSNP:186780790
2028 2028 a, g dbSNP:200354838
2036 2036 c, t dbSNP:530920048
2038 2038 a, g dbSNP:775333759
2042 2042 c, t dbSNP:771881586
2043 2043 g, t dbSNP:745708633
2045 2045 a, g dbSNP:779273607
2051 2051 c, t dbSNP:201497094
2058 2058 a, g dbSNP:749324850
2059 2059 -, ca dbSNP:763422136
2068 2068 c, t dbSNP:201997498
2072 2072 a, g dbSNP:756537403
2076 2076 -, aagaga dbSNP:112808902
2076 2076 -, aa dbSNP:773782075
2079 2079 a, g dbSNP:10850326
2082 2082 g, t dbSNP:371119204
2084 2084 a, g dbSNP:377033399
2089 2089 -, ag dbSNP:201961625
2090 2090 -, ag, agag dbSNP:762460052
2103 2103 -, ga dbSNP:768653470
2104 2104 -, aag dbSNP:747209673
2109 2109 a, g dbSNP:541667882
2116 2116 a, g dbSNP:28730761
2117 2117 g, t dbSNP:764799644
2121 2121 a, c dbSNP:761479218
2127 2127 c, g dbSNP:753308282
2129 2129 a, t dbSNP:763633224
2132 2132 c, t dbSNP:76633287
2133 2133 c, g dbSNP:76347803
2134 2134 a, c dbSNP:78828439
2135 2135 -, cg dbSNP:780156117
2135 2135 a, c dbSNP:76694710
2136 2136 a, g dbSNP:883079
2142 2142 c, g dbSNP:543786091
2144 2144 c, t dbSNP:771970874
2160 2160 a, g dbSNP:112051831
2168 2168 a, c dbSNP:28730760
2169 2169 a, t dbSNP:371880710
2174 2174 a, t dbSNP:771227659
2178 2178 a, g dbSNP:749552000
2183 2183 c, t dbSNP:535494778
2218 2218 c, g dbSNP:181130921
2225 2225 a, g dbSNP:769894729
2307 2307 c, t dbSNP:2384410
2329 2329 a, g dbSNP:370109574
2334 2334 c, t dbSNP:552724647
2345 2345 c, t dbSNP:571901755
2392 2392 a, g dbSNP:745450726
2423 2423 c, t dbSNP:12426660
2446 2446 a, g dbSNP:757238614
2465 2465 g, t dbSNP:552425924
2516 2516 -, t dbSNP:151183753
2518 2518 a, g dbSNP:536993995
2519 2519 a, g dbSNP:569991822
2535 2535 a, g dbSNP:552017501
2556 2556 c, t dbSNP:773913802
2560 2560 a, g dbSNP:758948929
2563 2563 a, t dbSNP:76799455
2566 2566 c, g dbSNP:566193674
2602 2602 a, g dbSNP:78058633
2611 2611 a, t dbSNP:189764599
2640 2640 a, g dbSNP:116382074
2702 2702 a, c dbSNP:559194135
2708 2708 c, t dbSNP:766553397
2712 2712 c, t dbSNP:140532076
2721 2721 c, g dbSNP:531923731
2722 2722 c, t dbSNP:563250446
2735 2735 a, g dbSNP:541449392
2741 2741 c, t dbSNP:759107305
2756 2756 c, t dbSNP:148346089
2775 2775 c, t dbSNP:552634822
2777 2777 a, c dbSNP:553621044
2778 2778 c, t dbSNP:117414057
2823 2823 a, g dbSNP:776283407
2851 2851 c, t dbSNP:143511878
2860 2860 c, t dbSNP:760503694
2875 2875 a, c dbSNP:537303672
2881 2881 c, t dbSNP:569872023
2926 2926 a, g dbSNP:571557978
2939 2939 c, t dbSNP:773180889
2948 2948 a, g dbSNP:184360838
2983 2983 a, t dbSNP:536633001
2999 2999 a, g dbSNP:566107581
3004 3004 g, t dbSNP:547907580
3010 3010 c, g dbSNP:777236533
3058 3058 c, t dbSNP:764387876
3102 3102 c, t dbSNP:192445542
3105 3105 c, t dbSNP:188337192
3124 3124 c, t dbSNP:549280585
3133 3133 a, c dbSNP:184793497
3140 3140 a, g dbSNP:6489956
3227 3227 c, t dbSNP:141223729
3237 3237 a, g dbSNP:557604192
3250 3250 a, g dbSNP:532005395
3292 3292 c, t dbSNP:561439634
3351 3351 c, t dbSNP:542952088
3357 3357 -, gttttttt dbSNP:776263824
3366 3366 -, t dbSNP:201799304
3367 3367 -, t dbSNP:373833293
3370 3370 g, t dbSNP:535072534
3374 3374 -, t dbSNP:201166554
3396 3396 c, t dbSNP:147774482
3412 3412 g, t dbSNP:192527148
3414 3414 -, a dbSNP:199773239
3415 3415 a, t dbSNP:75334514
3424 3424 c, t dbSNP:200407853
3424 3424 -, t dbSNP:35534655
3426 3426 c, t dbSNP:202095845
3485 3485 g, t dbSNP:576867150
3506 3506 c, g dbSNP:558688524
3516 3516 -, aagt dbSNP:564562667
3534 3534 c, g dbSNP:187379531
3559 3559 g, t dbSNP:183595902
3570 3570 -, g dbSNP:34050413
3620 3620 a, g dbSNP:747205317

Target ORF information:

RefSeq Version NM_080717
Organism Homo sapiens (human)
Definition Homo sapiens T-box 5 (TBX5), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_080717

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGAGGGAA TCAAAGTGTT TCTCCATGAA AGAGAACTGT GGCTAAAATT CCACGAAGTG 
GGCACGGAAA TGATCATAAC CAAGGCTGGA AGGCGGATGT TTCCCAGTTA CAAAGTGAAG
GTGACGGGCC TTAATCCCAA AACGAAGTAC ATTCTTCTCA TGGACATTGT ACCTGCCGAC
GATCACAGAT ACAAATTCGC AGATAATAAA TGGTCTGTGA CGGGCAAAGC TGAGCCCGCC
ATGCCTGGCC GCCTGTACGT GCACCCAGAC TCCCCCGCCA CCGGGGCGCA TTGGATGAGG
CAGCTCGTCT CCTTCCAGAA ACTCAAGCTC ACCAACAACC ACCTGGACCC ATTTGGGCAT
ATTATTCTAA ATTCCATGCA CAAATACCAG CCTAGATTAC ACATCGTGAA AGCGGATGAA
AATAATGGAT TTGGCTCAAA AAATACAGCG TTCTGCACTC ACGTCTTTCC TGAGACTGCG
TTTATAGCAG TGACTTCCTA CCAGAACCAC AAGATCACGC AATTAAAGAT TGAGAATAAT
CCCTTTGCCA AAGGATTTCG GGGCAGTGAT GACATGGAGC TGCACAGAAT GTCAAGAATG
CAAAGTAAAG AATATCCCGT GGTCCCCAGG AGCACCGTGA GGCAAAAAGT GGCCTCCAAC
CACAGTCCTT TCAGCAGCGA GTCTCGAGCT CTCTCCACCT CATCCAATTT GGGGTCCCAA
TACCAGTGTG AGAATGGTGT TTCCGGCCCC TCCCAGGACC TCCTGCCTCC ACCCAACCCA
TACCCACTGC CCCAGGAGCA TAGCCAAATT TACCATTGTA CCAAGAGGAA AGAGGAAGAA
TGTTCCACCA CAGACCATCC CTATAAGAAG CCCTACATGG AGACATCACC CAGTGAAGAA
GATTCCTTCT ACCGCTCTAG CTATCCACAG CAGCAGGGCC TGGGTGCCTC CTACAGGACA
GAGTCGGCAC AGCGGCAAGC TTGCATGTAT GCCAGCTCTG CGCCCCCCAG CGAGCCTGTG
CCCAGCCTAG AGGACATCAG CTGCAACACG TGGCCAAGCA TGCCTTCCTA CAGCAGCTGC
ACCGTCACCA CCGTGCAGCC CATGGACAGG CTACCCTACC AGCACTTCTC CGCTCACTTC
ACCTCGGGGC CCCTGGTCCC TCGGCTGGCT GGCATGGCCA ACCATGGCTC CCCACAGCTG
GGAGAGGGAA TGTTCCAGCA CCAGACCTCC GTGGCCCACC AGCCTGTGGT CAGGCAGTGT
GGGCCTCAGA CTGGCCTGCA GTCCCCTGGC ACCCTTCAGC CCCCTGAGTT CCTCTACTCT
CATGGCGTGC CAAGGACTCT ATCCCCTCAT CAGTACCACT CTGTGCACGG AGTTGGCATG
GTGCCAGAGT GGAGCGACAA TAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20722
Accession Version NM_181486.2
Sequence Information ORF Nucleotide Sequence (Length: 1557bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product T-box transcription factor TBX5 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB960757.1, BC027942.1 and AC009260.13. On May 12, 2012 this sequence version replaced gi:31652231. Summary: This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1 and 4 both encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC027942.1, AK290611.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGACGAGGG CTTTGGCCTG GCGCACACGC CTCTGGAGCC TGACGCAAAA 
GACCTGCCCT GCGATTCGAA ACCCGAGAGC GCGCTCGGGG CCCCCAGCAA GTCCCCGTCG
TCCCCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAGGGAA TCAAAGTGTT TCTCCATGAA
AGAGAACTGT GGCTAAAATT CCACGAAGTG GGCACGGAAA TGATCATAAC CAAGGCTGGA
AGGCGGATGT TTCCCAGTTA CAAAGTGAAG GTGACGGGCC TTAATCCCAA AACGAAGTAC
ATTCTTCTCA TGGACATTGT ACCTGCCGAC GATCACAGAT ACAAATTCGC AGATAATAAA
TGGTCTGTGA CGGGCAAAGC TGAGCCCGCC ATGCCTGGCC GCCTGTACGT GCACCCAGAC
TCCCCCGCCA CCGGGGCGCA TTGGATGAGG CAGCTCGTCT CCTTCCAGAA ACTCAAGCTC
ACCAACAACC ACCTGGACCC ATTTGGGCAT ATTATTCTAA ATTCCATGCA CAAATACCAG
CCTAGATTAC ACATCGTGAA AGCGGATGAA AATAATGGAT TTGGCTCAAA AAATACAGCG
TTCTGCACTC ACGTCTTTCC TGAGACTGCG TTTATAGCAG TGACTTCCTA CCAGAACCAC
AAGATCACGC AATTAAAGAT TGAGAATAAT CCCTTTGCCA AAGGATTTCG GGGCAGTGAT
GACATGGAGC TGCACAGAAT GTCAAGAATG CAAAGTAAAG AATATCCCGT GGTCCCCAGG
AGCACCGTGA GGCAAAAAGT GGCCTCCAAC CACAGTCCTT TCAGCAGCGA GTCTCGAGCT
CTCTCCACCT CATCCAATTT GGGGTCCCAA TACCAGTGTG AGAATGGTGT TTCCGGCCCC
TCCCAGGACC TCCTGCCTCC ACCCAACCCA TACCCACTGC CCCAGGAGCA TAGCCAAATT
TACCATTGTA CCAAGAGGAA AGAGGAAGAA TGTTCCACCA CAGACCATCC CTATAAGAAG
CCCTACATGG AGACATCACC CAGTGAAGAA GATTCCTTCT ACCGCTCTAG CTATCCACAG
CAGCAGGGCC TGGGTGCCTC CTACAGGACA GAGTCGGCAC AGCGGCAAGC TTGCATGTAT
GCCAGCTCTG CGCCCCCCAG CGAGCCTGTG CCCAGCCTAG AGGACATCAG CTGCAACACG
TGGCCAAGCA TGCCTTCCTA CAGCAGCTGC ACCGTCACCA CCGTGCAGCC CATGGACAGG
CTACCCTACC AGCACTTCTC CGCTCACTTC ACCTCGGGGC CCCTGGTCCC TCGGCTGGCT
GGCATGGCCA ACCATGGCTC CCCACAGCTG GGAGAGGGAA TGTTCCAGCA CCAGACCTCC
GTGGCCCACC AGCCTGTGGT CAGGCAGTGT GGGCCTCAGA CTGGCCTGCA GTCCCCTGGC
ACCCTTCAGC CCCCTGAGTT CCTCTACTCT CATGGCGTGC CAAGGACTCT ATCCCCTCAT
CAGTACCACT CTGTGCACGG AGTTGGCATG GTGCCAGAGT GGAGCGACAA TAGCTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_852259.1
CDS575..2131
Misc Feature(1)437..439(+)
Misc Feature(2)731..1297(+)
Misc Feature(3)797..1288(+)
Misc Feature(4)869..1138(+)
Exon (1)1..536
Gene:TBX5
Gene Synonym:
Exon (2)537..721
Gene:TBX5
Gene Synonym:
Exon (3)722..816
Gene:TBX5
Gene Synonym:
Exon (4)817..936
Gene:TBX5
Gene Synonym:
Exon (5)937..1084
Gene:TBX5
Gene Synonym:
Exon (6)1085..1237
Gene:TBX5
Gene Synonym:
Exon (7)1238..1329
Gene:TBX5
Gene Synonym:
Exon (8)1330..1556
Gene:TBX5
Gene Synonym:
Exon (9)1557..3733
Gene:TBX5
Gene Synonym:
Translation
Position Chain Variation Link
13 13 a, c dbSNP:553240209
15 15 a, g, t dbSNP:185270151
29 29 c, t dbSNP:751446359
61 61 c, t dbSNP:541207525
72 72 c, g dbSNP:577060348
91 91 a, g dbSNP:546736241
95 95 a, g dbSNP:558793514
97 97 -, ag dbSNP:372595886
102 102 -, ggag dbSNP:561423312
115 115 a, c dbSNP:11067100
212 212 a, g dbSNP:757482684
223 223 a, g dbSNP:139857351
230 230 a, g dbSNP:754094584
244 244 a, t dbSNP:545364029
250 250 c, t dbSNP:764021679
267 267 a, g dbSNP:181069109
276 276 c, g dbSNP:760764305
288 288 g, t dbSNP:753164916
304 304 g, t dbSNP:535146392
307 307 c, t dbSNP:573249367
326 326 c, t dbSNP:546752009
363 363 a, g dbSNP:12423887
368 368 g, t dbSNP:570678278
376 376 c, t dbSNP:145944567
385 385 a, c dbSNP:759872890
407 407 c, t dbSNP:774605490
450 450 c, t dbSNP:774636402
462 462 a, t dbSNP:530841550
540 540 a, g dbSNP:763178387
549 549 a, c, g dbSNP:769895888
550 550 g, t dbSNP:748222016
551 551 a, c, t dbSNP:769107196
552 552 c, g, t dbSNP:571328934
554 554 -, g dbSNP:750305087
556 556 -, acaggg dbSNP:762161797
560 560 a, g dbSNP:756902625
562 562 c, t dbSNP:550201404
563 563 c, t dbSNP:777370158
570 570 g, t dbSNP:755664220
580 580 c, t dbSNP:752191065
583 583 c, g dbSNP:774750288
589 589 c, t dbSNP:767487291
590 590 a, g dbSNP:145365553
594 594 a, g dbSNP:751312844
597 597 c, t dbSNP:766170094
612 612 a, c dbSNP:763125466
614 614 a, c dbSNP:773397553
625 625 c, t dbSNP:765479608
629 629 g, t dbSNP:200461617
631 631 a, c, g dbSNP:567785340
642 642 a, c, t dbSNP:141609745
645 645 g, t dbSNP:772587337
646 646 c, t dbSNP:746284842
647 647 g, t dbSNP:148195311
657 657 c, t dbSNP:755790672
661 661 a, g dbSNP:527914495
667 667 g, t dbSNP:780584178
671 671 a, g, t dbSNP:751532982
673 673 a, g dbSNP:372557517
674 674 a, g, t dbSNP:368311885
675 675 a, c, t dbSNP:761952835
678 678 a, c dbSNP:776705102
688 688 c, g, t dbSNP:34014008
689 689 c, t dbSNP:145703644
691 691 a, g dbSNP:149030937
699 699 c, t dbSNP:759976245
715 715 a, c, g, t dbSNP:564730479
718 718 a, g dbSNP:780717533
719 719 a, c dbSNP:104894383
724 724 c, t dbSNP:768418027
727 727 a, g dbSNP:746504712
730 730 g, t dbSNP:771883815
731 731 a, g dbSNP:779550194
732 732 a, g dbSNP:369919453
734 734 a, g dbSNP:145890934
735 735 c, t dbSNP:104894384
742 742 a, g dbSNP:745834660
743 743 a, t dbSNP:562706670
751 751 c, t dbSNP:544138286
757 757 a, c, g dbSNP:777853147
758 758 a, g dbSNP:756464860
775 775 c, t dbSNP:752768742
778 778 c, t dbSNP:375854421
779 779 g, t dbSNP:104894377
781 781 a, c dbSNP:760082444
790 790 a, g dbSNP:752033521
791 791 -, a dbSNP:35857654
808 808 a, g dbSNP:766962105
810 810 c, t dbSNP:763219727
812 812 a, g dbSNP:104894381
835 835 c, t dbSNP:767197919
838 838 a, g dbSNP:758964536
839 839 a, g dbSNP:778885169
846 846 c, t dbSNP:775593443
850 850 c, g dbSNP:773971213
851 851 a, g dbSNP:770717773
863 863 a, g dbSNP:749255660
867 867 a, c dbSNP:772844823
868 868 a, g dbSNP:769736865
875 875 a, t dbSNP:515726234
881 881 c, g dbSNP:748009824
883 883 c, t dbSNP:28730763
889 889 c, t dbSNP:745985270
890 890 a, g dbSNP:147710408
892 892 c, t dbSNP:141457646
896 896 a, c, t dbSNP:376621016
901 901 c, t dbSNP:749667596
905 905 g, t dbSNP:77357563
912 912 a, g dbSNP:483353129
914 914 c, t dbSNP:757586102
924 924 c, t dbSNP:754267671
928 928 c, t dbSNP:151249768
930 930 a, g dbSNP:556671717
933 933 a, g dbSNP:750969565
941 941 a, g dbSNP:754145043
946 946 a, g dbSNP:778117002
949 949 c, t dbSNP:142293206
964 964 c, t dbSNP:751249347
982 982 a, c dbSNP:104894379
983 983 a, g dbSNP:765999767
994 994 c, g dbSNP:148715804
1001 1001 a, g dbSNP:374906778
1006 1006 a, c, t dbSNP:146038442
1009 1009 a, g dbSNP:761842850
1012 1012 c, g dbSNP:776523515
1014 1014 a, g dbSNP:768515930
1024 1024 g, t dbSNP:760434419
1030 1030 c, t dbSNP:775748269
1031 1031 a, g dbSNP:551461950
1032 1032 a, t dbSNP:772248871
1036 1036 a, c dbSNP:745934847
1039 1039 c, t dbSNP:778977800
1041 1041 a, t dbSNP:771361403
1045 1045 a, g dbSNP:749827599
1054 1054 c, t dbSNP:778064192
1057 1057 c, g dbSNP:756481812
1077 1077 c, t dbSNP:753047224
1114 1114 a, g dbSNP:770274405
1129 1129 c, t dbSNP:142631741
1130 1130 a, g dbSNP:557183241
1137 1137 c, t dbSNP:758079210
1138 1138 a, g, t dbSNP:745378130
1150 1150 c, t dbSNP:201212120
1158 1158 c, g dbSNP:564042302
1159 1159 c, t dbSNP:753457483
1173 1173 a, c dbSNP:370771953
1174 1174 a, g, t dbSNP:139329918
1177 1177 c, t dbSNP:767238220
1178 1178 c, t dbSNP:759713411
1180 1180 c, t dbSNP:774522949
1183 1183 c, t dbSNP:146230255
1184 1184 c, g dbSNP:763004247
1186 1186 c, t dbSNP:200326827
1187 1187 a, g, t dbSNP:190881877
1189 1189 c, g dbSNP:200921127
1192 1192 c, g, t dbSNP:533581420
1198 1198 c, g dbSNP:769113870
1201 1201 g, t dbSNP:747429505
1213 1213 a, g dbSNP:149274578
1225 1225 c, t dbSNP:201394336
1231 1231 c, t dbSNP:778217968
1235 1235 a, g dbSNP:757039207
1237 1237 c, g dbSNP:748766700
1243 1243 a, g dbSNP:376519728
1247 1247 c, t dbSNP:765547666
1270 1270 c, t dbSNP:747652988
1283 1283 c, t dbSNP:104894382
1284 1284 a, g dbSNP:104894378
1285 1285 a, g dbSNP:754018766
1288 1288 c, t dbSNP:764630371
1289 1289 a, g dbSNP:761278614
1297 1297 c, t dbSNP:368563052
1298 1298 a, g dbSNP:772595679
1301 1301 a, g dbSNP:759983816
1303 1303 g, t dbSNP:186183947
1306 1306 g, t dbSNP:769541948
1310 1310 a, c, g dbSNP:374600913
1321 1321 a, g dbSNP:138552878
1342 1342 c, t dbSNP:370805715
1343 1343 a, g dbSNP:200382742
1355 1355 a, t dbSNP:377625550
1357 1357 -, cccaggagc dbSNP:766716477
1360 1360 c, t dbSNP:375955080
1361 1361 a, g dbSNP:147405081
1365 1365 a, g dbSNP:201071418
1369 1369 a, g dbSNP:778784399
1372 1372 a, t dbSNP:777287804
1373 1373 a, g dbSNP:757373962
1378 1378 c, g dbSNP:35110399
1387 1387 c, t dbSNP:778180226
1391 1391 a, c dbSNP:756238293
1392 1392 c, g dbSNP:752879569
1401 1401 a, g dbSNP:147977741
1402 1402 c, t dbSNP:146908258
1403 1403 a, g dbSNP:369697961
1408 1408 c, t dbSNP:142609815
1410 1410 a, g dbSNP:115178276
1411 1411 a, c dbSNP:564499369
1414 1414 c, t dbSNP:763797226
1415 1415 c, t dbSNP:760177359
1416 1416 g, t dbSNP:774986848
1422 1422 a, c dbSNP:147077037
1428 1428 c, t dbSNP:537547818
1429 1429 c, t dbSNP:745840026
1433 1433 c, t dbSNP:773978838
1438 1438 g, t dbSNP:770728362
1439 1439 g, t dbSNP:543015692
1441 1441 c, t dbSNP:778126616
1468 1468 c, t dbSNP:770206057
1469 1469 a, g dbSNP:201637366
1470 1470 g, t dbSNP:781342709
1474 1474 c, t dbSNP:755387654
1484 1484 c, t dbSNP:752134526
1488 1488 c, g, t dbSNP:758752836
1492 1492 a, t dbSNP:750855891
1493 1493 c, t dbSNP:763672657
1496 1496 c, t dbSNP:760334883
1501 1501 a, c dbSNP:201164281
1518 1518 a, g dbSNP:752101162
1522 1522 a, g dbSNP:767086503
1539 1539 a, g dbSNP:759009256
1548 1548 a, g dbSNP:774321607
1558 1558 a, g dbSNP:750350579
1559 1559 a, g dbSNP:142807139
1565 1565 a, t dbSNP:765327234
1569 1569 c, t dbSNP:374446377
1578 1578 a, g dbSNP:776297033
1587 1587 a, g dbSNP:764322332
1589 1589 a, g dbSNP:760830193
1594 1594 a, g dbSNP:148977955
1596 1596 c, g dbSNP:772109169
1602 1602 c, t dbSNP:745997257
1603 1603 a, g dbSNP:774825317
1606 1606 a, g dbSNP:771408470
1608 1608 a, c dbSNP:749598173
1613 1613 a, c dbSNP:778011764
1637 1637 c, t dbSNP:372827955
1638 1638 a, g, t dbSNP:145784562
1650 1650 c, t dbSNP:149792175
1656 1656 a, g dbSNP:140945366
1658 1658 c, g dbSNP:765204502
1675 1675 c, g dbSNP:763029336
1687 1687 a, g dbSNP:757158327
1688 1688 a, t dbSNP:79040021
1689 1689 c, t dbSNP:143068551
1690 1690 a, g dbSNP:763827720
1697 1697 c, t dbSNP:377532269
1699 1699 g, t dbSNP:775667911
1709 1709 a, c, t dbSNP:759607221
1711 1711 c, g dbSNP:774419025
1714 1714 c, t dbSNP:771409350
1716 1716 a, c dbSNP:763390789
1717 1717 a, c dbSNP:773582799
1725 1725 c, t dbSNP:530882236
1726 1726 a, g dbSNP:200073406
1731 1731 a, c, t dbSNP:771857554
1732 1732 c, t dbSNP:745319218
1735 1735 a, c, g, t dbSNP:753688559
1736 1736 a, g dbSNP:139371720
1745 1745 c, t dbSNP:755849744
1753 1753 a, g dbSNP:752553883
1758 1758 a, g dbSNP:146269931
1773 1773 c, t dbSNP:759766836
1774 1774 a, g dbSNP:751588696
1777 1777 g, t dbSNP:377649723
1780 1780 a, c dbSNP:763129045
1782 1782 c, g dbSNP:773568096
1789 1789 a, t dbSNP:770387292
1798 1798 a, c dbSNP:762204624
1806 1806 c, t dbSNP:267603320
1807 1807 c, g, t dbSNP:188839350
1808 1808 a, c, g dbSNP:114124210
1816 1816 c, t dbSNP:370133092
1817 1817 a, g dbSNP:748889317
1834 1834 c, g dbSNP:780330016
1837 1837 a, g dbSNP:777568811
1843 1843 c, t dbSNP:756049331
1844 1844 a, c, g dbSNP:780987688
1847 1847 a, c dbSNP:755152246
1852 1852 c, t dbSNP:751745926
1855 1855 c, t dbSNP:6489957
1856 1856 a, g, t dbSNP:577615373
1867 1867 c, g dbSNP:143971376
1870 1870 a, g dbSNP:149474574
1873 1873 g, t dbSNP:777099745
1879 1879 g, t dbSNP:372185479
1886 1886 c, t dbSNP:761170474
1887 1887 a, g dbSNP:557758851
1893 1893 c, t dbSNP:770685602
1915 1915 c, g dbSNP:748678010
1917 1917 c, t dbSNP:772790458
1918 1918 a, g dbSNP:769669494
1925 1925 a, g dbSNP:200704209
1932 1932 a, g dbSNP:781143449
1949 1949 a, g dbSNP:754740593
1954 1954 c, t dbSNP:575162861
1955 1955 a, g dbSNP:201152128
1958 1958 a, g dbSNP:758663811
1962 1962 a, c dbSNP:750423209
1970 1970 a, g dbSNP:765443283
1974 1974 c, t dbSNP:757457183
1981 1981 c, g dbSNP:373007088
1987 1987 a, g dbSNP:764756835
1989 1989 c, t dbSNP:761119136
1993 1993 a, g dbSNP:1048078
1999 1999 c, g, t dbSNP:199729532
2002 2002 a, g dbSNP:762687473
2016 2016 c, t dbSNP:772556634
2017 2017 c, t dbSNP:137928706
2023 2023 a, g dbSNP:117965596
2025 2025 a, c, t dbSNP:768644203
2027 2027 c, g dbSNP:746726123
2037 2037 c, t dbSNP:779923573
2050 2050 a, c, t dbSNP:369034176
2051 2051 a, g dbSNP:556197042
2060 2060 a, g dbSNP:757399952
2064 2064 c, t dbSNP:374676021
2065 2065 a, g dbSNP:778359709
2069 2069 c, g dbSNP:756656267
2080 2080 c, t dbSNP:752953396
2092 2092 c, t dbSNP:767955877
2093 2093 a, g dbSNP:374924325
2097 2097 c, t dbSNP:147339942
2103 2103 a, t dbSNP:764923882
2119 2119 a, c, t dbSNP:186780790
2120 2120 a, g dbSNP:200354838
2128 2128 c, t dbSNP:530920048
2130 2130 a, g dbSNP:775333759
2134 2134 c, t dbSNP:771881586
2135 2135 g, t dbSNP:745708633
2137 2137 a, g dbSNP:779273607
2143 2143 c, t dbSNP:201497094
2150 2150 a, g dbSNP:749324850
2151 2151 -, ca dbSNP:763422136
2160 2160 c, t dbSNP:201997498
2164 2164 a, g dbSNP:756537403
2168 2168 -, aagaga dbSNP:112808902
2168 2168 -, aa dbSNP:773782075
2171 2171 a, g dbSNP:10850326
2174 2174 g, t dbSNP:371119204
2176 2176 a, g dbSNP:377033399
2181 2181 -, ag dbSNP:201961625
2182 2182 -, ag, agag dbSNP:762460052
2195 2195 -, ga dbSNP:768653470
2196 2196 -, aag dbSNP:747209673
2201 2201 a, g dbSNP:541667882
2208 2208 a, g dbSNP:28730761
2209 2209 g, t dbSNP:764799644
2213 2213 a, c dbSNP:761479218
2219 2219 c, g dbSNP:753308282
2221 2221 a, t dbSNP:763633224
2224 2224 c, t dbSNP:76633287
2225 2225 c, g dbSNP:76347803
2226 2226 a, c dbSNP:78828439
2227 2227 -, cg dbSNP:780156117
2227 2227 a, c dbSNP:76694710
2228 2228 a, g dbSNP:883079
2234 2234 c, g dbSNP:543786091
2236 2236 c, t dbSNP:771970874
2252 2252 a, g dbSNP:112051831
2260 2260 a, c dbSNP:28730760
2261 2261 a, t dbSNP:371880710
2266 2266 a, t dbSNP:771227659
2270 2270 a, g dbSNP:749552000
2275 2275 c, t dbSNP:535494778
2310 2310 c, g dbSNP:181130921
2317 2317 a, g dbSNP:769894729
2399 2399 c, t dbSNP:2384410
2421 2421 a, g dbSNP:370109574
2426 2426 c, t dbSNP:552724647
2437 2437 c, t dbSNP:571901755
2484 2484 a, g dbSNP:745450726
2515 2515 c, t dbSNP:12426660
2538 2538 a, g dbSNP:757238614
2557 2557 g, t dbSNP:552425924
2608 2608 -, t dbSNP:151183753
2610 2610 a, g dbSNP:536993995
2611 2611 a, g dbSNP:569991822
2627 2627 a, g dbSNP:552017501
2648 2648 c, t dbSNP:773913802
2652 2652 a, g dbSNP:758948929
2655 2655 a, t dbSNP:76799455
2658 2658 c, g dbSNP:566193674
2694 2694 a, g dbSNP:78058633
2703 2703 a, t dbSNP:189764599
2732 2732 a, g dbSNP:116382074
2794 2794 a, c dbSNP:559194135
2800 2800 c, t dbSNP:766553397
2804 2804 c, t dbSNP:140532076
2813 2813 c, g dbSNP:531923731
2814 2814 c, t dbSNP:563250446
2827 2827 a, g dbSNP:541449392
2833 2833 c, t dbSNP:759107305
2848 2848 c, t dbSNP:148346089
2867 2867 c, t dbSNP:552634822
2869 2869 a, c dbSNP:553621044
2870 2870 c, t dbSNP:117414057
2915 2915 a, g dbSNP:776283407
2943 2943 c, t dbSNP:143511878
2952 2952 c, t dbSNP:760503694
2967 2967 a, c dbSNP:537303672
2973 2973 c, t dbSNP:569872023
3018 3018 a, g dbSNP:571557978
3031 3031 c, t dbSNP:773180889
3040 3040 a, g dbSNP:184360838
3075 3075 a, t dbSNP:536633001
3091 3091 a, g dbSNP:566107581
3096 3096 g, t dbSNP:547907580
3102 3102 c, g dbSNP:777236533
3150 3150 c, t dbSNP:764387876
3194 3194 c, t dbSNP:192445542
3197 3197 c, t dbSNP:188337192
3216 3216 c, t dbSNP:549280585
3225 3225 a, c dbSNP:184793497
3232 3232 a, g dbSNP:6489956
3319 3319 c, t dbSNP:141223729
3329 3329 a, g dbSNP:557604192
3342 3342 a, g dbSNP:532005395
3384 3384 c, t dbSNP:561439634
3443 3443 c, t dbSNP:542952088
3449 3449 -, gttttttt dbSNP:776263824
3458 3458 -, t dbSNP:201799304
3459 3459 -, t dbSNP:373833293
3462 3462 g, t dbSNP:535072534
3466 3466 -, t dbSNP:201166554
3488 3488 c, t dbSNP:147774482
3504 3504 g, t dbSNP:192527148
3506 3506 -, a dbSNP:199773239
3507 3507 a, t dbSNP:75334514
3516 3516 c, t dbSNP:200407853
3516 3516 -, t dbSNP:35534655
3518 3518 c, t dbSNP:202095845
3577 3577 g, t dbSNP:576867150
3598 3598 c, g dbSNP:558688524
3608 3608 -, aagt dbSNP:564562667
3626 3626 c, g dbSNP:187379531
3651 3651 g, t dbSNP:183595902
3662 3662 -, g dbSNP:34050413
3712 3712 a, g dbSNP:747205317

Target ORF information:

RefSeq Version NM_181486
Organism Homo sapiens (human)
Definition Homo sapiens T-box 5 (TBX5), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_181486

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGCCGACG CAGACGAGGG CTTTGGCCTG GCGCACACGC CTCTGGAGCC TGACGCAAAA 
GACCTGCCCT GCGATTCGAA ACCCGAGAGC GCGCTCGGGG CCCCCAGCAA GTCCCCGTCG
TCCCCGCAGG CCGCCTTCAC CCAGCAGGGC ATGGAGGGAA TCAAAGTGTT TCTCCATGAA
AGAGAACTGT GGCTAAAATT CCACGAAGTG GGCACGGAAA TGATCATAAC CAAGGCTGGA
AGGCGGATGT TTCCCAGTTA CAAAGTGAAG GTGACGGGCC TTAATCCCAA AACGAAGTAC
ATTCTTCTCA TGGACATTGT ACCTGCCGAC GATCACAGAT ACAAATTCGC AGATAATAAA
TGGTCTGTGA CGGGCAAAGC TGAGCCCGCC ATGCCTGGCC GCCTGTACGT GCACCCAGAC
TCCCCCGCCA CCGGGGCGCA TTGGATGAGG CAGCTCGTCT CCTTCCAGAA ACTCAAGCTC
ACCAACAACC ACCTGGACCC ATTTGGGCAT ATTATTCTAA ATTCCATGCA CAAATACCAG
CCTAGATTAC ACATCGTGAA AGCGGATGAA AATAATGGAT TTGGCTCAAA AAATACAGCG
TTCTGCACTC ACGTCTTTCC TGAGACTGCG TTTATAGCAG TGACTTCCTA CCAGAACCAC
AAGATCACGC AATTAAAGAT TGAGAATAAT CCCTTTGCCA AAGGATTTCG GGGCAGTGAT
GACATGGAGC TGCACAGAAT GTCAAGAATG CAAAGTAAAG AATATCCCGT GGTCCCCAGG
AGCACCGTGA GGCAAAAAGT GGCCTCCAAC CACAGTCCTT TCAGCAGCGA GTCTCGAGCT
CTCTCCACCT CATCCAATTT GGGGTCCCAA TACCAGTGTG AGAATGGTGT TTCCGGCCCC
TCCCAGGACC TCCTGCCTCC ACCCAACCCA TACCCACTGC CCCAGGAGCA TAGCCAAATT
TACCATTGTA CCAAGAGGAA AGAGGAAGAA TGTTCCACCA CAGACCATCC CTATAAGAAG
CCCTACATGG AGACATCACC CAGTGAAGAA GATTCCTTCT ACCGCTCTAG CTATCCACAG
CAGCAGGGCC TGGGTGCCTC CTACAGGACA GAGTCGGCAC AGCGGCAAGC TTGCATGTAT
GCCAGCTCTG CGCCCCCCAG CGAGCCTGTG CCCAGCCTAG AGGACATCAG CTGCAACACG
TGGCCAAGCA TGCCTTCCTA CAGCAGCTGC ACCGTCACCA CCGTGCAGCC CATGGACAGG
CTACCCTACC AGCACTTCTC CGCTCACTTC ACCTCGGGGC CCCTGGTCCC TCGGCTGGCT
GGCATGGCCA ACCATGGCTC CCCACAGCTG GGAGAGGGAA TGTTCCAGCA CCAGACCTCC
GTGGCCCACC AGCCTGTGGT CAGGCAGTGT GGGCCTCAGA CTGGCCTGCA GTCCCCTGGC
ACCCTTCAGC CCCCTGAGTT CCTCTACTCT CATGGCGTGC CAAGGACTCT ATCCCCTCAT
CAGTACCACT CTGTGCACGG AGTTGGCATG GTGCCAGAGT GGAGCGACAA TAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.